RESUMEN
The renal glomerulus represents the major filtration body of the vertebrate nephron and is responsible for urine production and a number of other functions such as metabolic waste elimination and the regulation of water, electrolyte and acid-base balance. Podocytes are highly specialized epithelial cells that form a crucial part of the glomerular filtration barrier (GFB) by establishing a slit diaphragm for semipermeable plasma ultrafiltration. Defects of the GFB lead to proteinuria and impaired kidney function often resulting in end-stage renal failure. Although significant knowledge has been acquired in recent years, many aspects in podocyte biology are still incompletely understood. By using zebrafish as a vertebrate in vivo model, we report a novel role of the Kinesin-like motor protein Kif21a in glomerular filtration. Our studies demonstrate specific Kif21a localization to the podocytes. Its deficiency resulted in altered podocyte morphology leading to podocyte foot process effacement and altered slit diaphragm formation. Finally, we proved considerable functional consequences of Kif21a deficiency by demonstrating a leaky GFB resulting in severe proteinuria. Conclusively, our data identified a novel role of Kif21a for proper GFB function and adds another piece to the understanding of podocyte architecture and regulation.
Asunto(s)
Barrera de Filtración Glomerular , Cinesinas , Podocitos , Animales , Barrera de Filtración Glomerular/fisiopatología , Glomérulos Renales/metabolismo , Podocitos/metabolismo , Proteinuria/metabolismo , Pez Cebra , Cinesinas/genética , Proteínas de Pez Cebra/genéticaRESUMEN
Loss of normal kidney function affects more than 10% of the population and contributes to morbidity and mortality. Kidney diseases are currently treated with immunosuppressive agents, antihypertensives and diuretics with partial but limited success. Most kidney disease is characterized by breakdown of the glomerular filtration barrier (GFB). Specialized podocyte cells maintain the GFB, and structure-function experiments and studies of intercellular communication between the podocytes and other GFB cells, combined with advances from genetics and genomics, have laid the groundwork for a new generation of therapies that directly intervene at the GFB. These include inhibitors of apolipoprotein L1 (APOL1), short transient receptor potential channels (TRPCs), soluble fms-like tyrosine kinase 1 (sFLT1; also known as soluble vascular endothelial growth factor receptor 1), roundabout homologue 2 (ROBO2), endothelin receptor A, soluble urokinase plasminogen activator surface receptor (suPAR) and substrate intermediates for coenzyme Q10 (CoQ10). These molecular targets converge on two key components of GFB biology: mitochondrial function and the actin-myosin contractile machinery. This Review discusses therapies and developments focused on maintaining GFB integrity, and the emerging questions in this evolving field.
Asunto(s)
Barrera de Filtración Glomerular/efectos de los fármacos , Barrera de Filtración Glomerular/fisiología , Enfermedades Renales/tratamiento farmacológico , Enfermedades Renales/fisiopatología , Animales , Barrera de Filtración Glomerular/fisiopatología , HumanosRESUMEN
BACKGROUND: Podocytes embrace the glomerular capillaries with foot processes, which are interconnected by a specialized adherens junction to ultimately form the filtration barrier. Altered adhesion and loss are common features of podocyte injury, which could be mediated by shedding of cell-adhesion molecules through the regulated activity of cell surface-expressed proteases. A Disintegrin and Metalloproteinase 10 (ADAM10) is such a protease known to mediate ectodomain shedding of adhesion molecules, among others. Here we evaluate the involvement of ADAM10 in the process of antibody-induced podocyte injury. METHODS: Membrane proteomics, immunoblotting, high-resolution microscopy, and immunogold electron microscopy were used to analyze human and murine podocyte ADAM10 expression in health and kidney injury. The functionality of ADAM10 ectodomain shedding for podocyte development and injury was analyzed, in vitro and in vivo, in the anti-podocyte nephritis (APN) model in podocyte-specific, ADAM10-deficient mice. RESULTS: ADAM10 is selectively localized at foot processes of murine podocytes and its expression is dispensable for podocyte development. Podocyte ADAM10 expression is induced in the setting of antibody-mediated injury in humans and mice. Podocyte ADAM10 deficiency attenuates the clinical course of APN and preserves the morphologic integrity of podocytes, despite subepithelial immune-deposit formation. Functionally, ADAM10-related ectodomain shedding results in cleavage of the cell-adhesion proteins N- and P-cadherin, thus decreasing their injury-related surface levels. This favors podocyte loss and the activation of downstream signaling events through the Wnt signaling pathway in an ADAM10-dependent manner. CONCLUSIONS: ADAM10-mediated ectodomain shedding of injury-related cadherins drives podocyte injury.
Asunto(s)
Proteína ADAM10/metabolismo , Secretasas de la Proteína Precursora del Amiloide/metabolismo , Proteínas de la Membrana/metabolismo , Nefritis/metabolismo , Síndrome Nefrótico/metabolismo , Podocitos/metabolismo , Podocitos/patología , Insuficiencia Renal Crónica/metabolismo , Proteína ADAM10/genética , Secretasas de la Proteína Precursora del Amiloide/genética , Animales , Autoanticuerpos/efectos adversos , Nitrógeno de la Urea Sanguínea , Cadherinas/metabolismo , Adhesión Celular , Comunicación Celular , Membrana Celular/metabolismo , Células Cultivadas , Creatinina/orina , Modelos Animales de Enfermedad , Femenino , Barrera de Filtración Glomerular/patología , Barrera de Filtración Glomerular/fisiopatología , Humanos , Masculino , Proteínas de la Membrana/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Nefritis/patología , Síndrome Nefrótico/patología , Podocitos/fisiología , Proteómica , Análisis de Matrices Tisulares , Transcriptoma , Vía de Señalización WntRESUMEN
The glomerular filtration barrier is a highly specialized capillary wall comprising fenestrated endothelial cells, podocytes, and an intervening basement membrane. In glomerular disease, this barrier loses functional integrity, allowing the passage of macromolecules and cells, and there are associated changes in both cell morphology and the extracellular matrix. Over the past 3 decades, there has been a transformation in our understanding about glomerular disease, fueled by genetic discovery, and this is leading to exciting advances in our knowledge about glomerular biology and pathophysiology. In current clinical practice, a genetic diagnosis already has important implications for management, ranging from estimating the risk of disease recurrence post-transplant to the life-changing advances in the treatment of atypical hemolytic uremic syndrome. Improving our understanding about the mechanistic basis of glomerular disease is required for more effective and personalized therapy options. In this review, we describe genotype and phenotype correlations for genetic disorders of the glomerular filtration barrier, with a particular emphasis on how these gene defects cluster by both their ontology and patterns of glomerular pathology.
Asunto(s)
Variación Genética , Barrera de Filtración Glomerular/patología , Glomerulonefritis/genética , Células Endoteliales/patología , Predisposición Genética a la Enfermedad , Membrana Basal Glomerular/patología , Barrera de Filtración Glomerular/fisiopatología , Glomerulonefritis/sangre , Glomerulonefritis/patología , Glomerulonefritis/fisiopatología , Humanos , Fenotipo , Podocitos/patología , Medición de Riesgo , Factores de RiesgoRESUMEN
Introducción: La enfermedad renal crónica expresada por un filtrado glomerular disminuido se asocia a un mayor riesgo de enfermedad cardiovascular y a mayor progresión del daño renal. Es muy recomendable como método disponible y económico para evaluar el riesgo cardiovascular en hipertensos. Objetivo: Evaluar el daño renal y la enfermedad cardiovascular en hipertensos del Policlínico Managua. Método: Se estudiaron 1037 pacientes hipertensos. Se calculó el filtrado glomerular según el Modification Diet Renal Disease Study. Se estudiaron variables demográficas, factores de riesgo vascular y presencia de enfermedad cardiovascular. Se definió 60 mil/min/1,73m2 para el filtrado glomerular disminuido. Resultados: De los pacientes estudiados, 28,5 por ciento presentaron filtrado glomerular disminuido, mostrando mayor prevalencia de enfermedad cardiovascular que aquellos sin daño renal (53,4 por ciento vs 33,9 por ciento; OR 2,19 IC 95 por ciento 1,51-2,82; p ; 0,001). Conclusiones: Este estudio muestra que la enfermedad renal crónica se asocia a mayor prevalencia de enfermedad cardiovascular, siendo un factor de riesgo cardiovascular mayor(AU)
Introduction: Chronic kidney disease expressed by decreased glomerular filtration rate is associated with high risk of cardiovascular disease and great progression of kidney damage. This filtration is highly recommended as an available and inexpensive method to assess cardiovascular risk in hypertensive patients. Objective: To assess kidney damage and cardiovascular disease in hypertensive patients at Managua Polyclinic. Method: This research included 1037 hypertensive patients. Glomerular filtration was calculated according to the Modification Diet Renal Disease Study. Demographic variables, vascular risk factors, and the presence of cardiovascular disease were studied. The decreased glomerular filtration was defined for ;60 thousand/min/1.73m2. Results: 28.5 percent of the studied patients showed decreased glomerular filtration, higher prevalence of cardiovascular disease than those without kidney damage (53.4 percent ;vs. 33.9 percent; OR 2.19 95 percent CI 1.51-2, 82; p ;0.001). Conclusions: This study showed that chronic kidney disease is associated with high prevalence of cardiovascular disease, which establishes higher cardiovascular risk factor(AU)
Asunto(s)
Humanos , Masculino , Femenino , Enfermedades Cardiovasculares/epidemiología , Insuficiencia Renal Crónica/complicaciones , Barrera de Filtración Glomerular/fisiopatología , Evaluación en Salud , Medición de Riesgo , Riesgo a la SaludRESUMEN
The podocyte is a key component of the glomerular filtration barrier. Podocyte dysfunction is central to the underlying pathophysiology of many common glomerular diseases, including diabetic nephropathy, glomerulonephritis and genetic forms of nephrotic syndrome. Collectively, these conditions affect millions of people worldwide, and account for the majority of kidney diseases requiring dialysis and transplantation. The 12th International Podocyte Conference was held in Montreal, Canada from May 30 to June 2, 2018. The primary aim of this conference was to bring together nephrologists, clinician scientists, basic scientists and their trainees from all over the world to present their research and to establish networks with the common goal of developing new therapies for glomerular diseases based on the latest advances in podocyte biology. This review briefly highlights recent advances made in understanding podocyte structure and metabolism, experimental systems in which to study podocytes and glomerular disease, disease mediators, genetic and immune origins of glomerulopathies, and the development of novel therapeutic agents to protect podocyte and glomerular injury.
Asunto(s)
Nefropatías Diabéticas/terapia , Barrera de Filtración Glomerular/fisiopatología , Glomerulonefritis/terapia , Síndrome Nefrótico/terapia , Podocitos/patología , Animales , Canadá , Congresos como Asunto , Nefropatías Diabéticas/patología , Nefropatías Diabéticas/fisiopatología , Glomerulonefritis/patología , Glomerulonefritis/fisiopatología , Humanos , Síndrome Nefrótico/patología , Síndrome Nefrótico/fisiopatologíaRESUMEN
Acute cardiorenal syndrome (CRS-1) is a morbid complication of acute cardiovascular disease. Heart-to-kidney signals transmitted by "cardiorenal connectors" have been postulated, but investigation into CRS-1 has been limited by technical limitations and a paucity of models. To address these limitations, we developed a translational model of CRS-1, cardiac arrest and cardiopulmonary resuscitation (CA/CPR), and now report findings from nanoscale mass spectrometry proteomic exploration of glomerular filtrate 2 hours after CA/CPR or sham procedure. Filtrate acquisition was confirmed by imaging, molecular weight and charge distribution, and exclusion of protein specific to surrounding cells. Filtration of proteins specific to the heart was detected following CA/CPR and confirmed with mass spectrometry performed using urine collections from mice with deficient tubular endocytosis. Cardiac LIM protein was a CA/CPR-specific filtrate component. Cardiac arrest induced plasma release of cardiac LIM protein in mice and critically ill human cardiac arrest survivors, and administration of recombinant cardiac LIM protein to mice altered renal function. These findings demonstrate that glomerular filtrate is accessible to nanoscale proteomics and elucidate the population of proteins filtered 2 hours after CA/CPR. The identification of cardiac-specific proteins in renal filtrate suggests a novel signaling mechanism in CRS-1. We expect these findings to advance understanding of CRS-1.
Asunto(s)
Síndrome Cardiorrenal/fisiopatología , Barrera de Filtración Glomerular/fisiopatología , Paro Cardíaco/complicaciones , Proteínas con Dominio LIM/metabolismo , Daño por Reperfusión/fisiopatología , Enfermedad Aguda , Animales , Biomarcadores/análisis , Biomarcadores/metabolismo , Síndrome Cardiorrenal/etiología , Síndrome Cardiorrenal/orina , Reanimación Cardiopulmonar , Línea Celular , Modelos Animales de Enfermedad , Barrera de Filtración Glomerular/diagnóstico por imagen , Barrera de Filtración Glomerular/metabolismo , Paro Cardíaco/terapia , Humanos , Microscopía Intravital , Proteínas con Dominio LIM/orina , Masculino , Espectrometría de Masas/métodos , Ratones , Podocitos , Proteómica/métodos , Daño por Reperfusión/etiología , Daño por Reperfusión/orinaRESUMEN
In many cells and tissues, including the glomerular filtration barrier, scaffold proteins are critical in optimizing signal transduction by enhancing structural stability and functionality of their ligands. Recently, mutations in scaffold protein membrane-associated guanylate kinase inverted 2 (MAGI-2) encoding gene were identified among the etiology of steroid-resistant nephrotic syndrome. MAGI-2 interacts with core proteins of multiple pathways, such as transforming growth factor-ß signaling, planar cell polarity pathway, and Wnt/ß-catenin signaling in podocyte and slit diaphragm. Through the interaction with its ligand, MAGI-2 modulates the regulation of apoptosis, cytoskeletal reorganization, and glomerular development. This review aims to summarize recent findings on the role of MAGI-2 and some other scaffold proteins, such as nephrin and synaptopodin, in the underlying mechanisms of glomerulopathy.
Asunto(s)
Proteínas Portadoras/metabolismo , Barrera de Filtración Glomerular/metabolismo , Tasa de Filtración Glomerular , Glomerulonefritis/metabolismo , Síndrome Nefrótico/metabolismo , Citoesqueleto de Actina/metabolismo , Citoesqueleto de Actina/patología , Proteínas Adaptadoras Transductoras de Señales , Animales , Apoptosis , Proteínas Portadoras/genética , Transición Epitelial-Mesenquimal , Predisposición Genética a la Enfermedad , Barrera de Filtración Glomerular/patología , Barrera de Filtración Glomerular/fisiopatología , Glomerulonefritis/genética , Glomerulonefritis/patología , Glomerulonefritis/fisiopatología , Guanilato-Quinasas , Humanos , Mutación , Síndrome Nefrótico/genética , Síndrome Nefrótico/patología , Síndrome Nefrótico/fisiopatología , Podocitos/metabolismo , Podocitos/patología , Transducción de SeñalRESUMEN
A systematic review was used to identify randomized controlled trials (RCTs) and observational epidemiologic studies (OBSs) that examined protein intake consistent with either the US RDA (0.8 g/kg or 10-15% of energy) or a higher protein intake (≥20% but <35% of energy or ≥10% higher than a comparison intake) and reported measures of kidney function. Studies (n = 26) of healthy, free-living adults (>18 y old) with or without metabolic disease risk factors were included. Studies of subjects with overt disease, such as chronic kidney, end-stage renal disease, cancer, or organ transplant, were excluded. The most commonly reported variable was glomerular filtration rate (GFR), with 13 RCTs comparing GFRs obtained with normal and higher protein intakes. Most (n = 8), but not all (n = 5), RCTs reported significantly higher GFRs in response to increased protein intake, and all rates were consistent with normal kidney function in healthy adults. The evidence from the current review is limited and inconsistent with regard to the role of protein intake and the risk of kidney stones. Increased protein intake had little or no effect on blood markers of kidney function. Evidence reported here suggests that protein intake above the US RDA has no adverse effect on blood pressure. All included studies were of moderate to high risk of bias and, with the exception of 2 included cohorts, were limited in duration (i.e. <6 mo). Data in the current review are insufficient to determine if increased protein intake from a particular source, i.e., plant or animal, influences kidney health outcomes. These data further indicate that, at least in the short term, higher protein intake within the range of recommended intakes for protein is consistent with normal kidney function in healthy individuals.
Asunto(s)
Proteínas en la Dieta/administración & dosificación , Enfermedades Renales/epidemiología , Estudios Observacionales como Asunto , Ensayos Clínicos Controlados Aleatorios como Asunto , Ingesta Diaria Recomendada , Adulto , Animales , Biomarcadores/sangre , Presión Sanguínea , Estudios Transversales , Ingestión de Energía , Femenino , Barrera de Filtración Glomerular/fisiopatología , Humanos , Enfermedades Renales/sangre , Enfermedades Renales/fisiopatología , Masculino , Persona de Mediana Edad , Proteínas de Plantas/administración & dosificación , ProteinuriaRESUMEN
Despite the effectiveness of renin-angiotensin blockade in retarding diabetic nephropathy progression, a considerable number of patients still develop end-stage renal disease. The present investigation aims to evaluate the protective potential of FPS-ZM1, a selective inhibitor of receptor for advanced glycation end products (RAGE), alone and in combination with valsartan, an angiotensin receptor blocker, against glomerular injury parameters in streptozotocin-induced diabetic rats. FPS-ZM1 at 1 mg/kg (i.p.), valsartan at 100 mg/kg (p.o.), and their combination were administered for 4 weeks, starting 2 months after diabetes induction in rats. Tests for kidney function, glomerular filtration barrier, and podocyte slit diaphragm integrities were performed. Combined FPS-ZM1/valsartan attenuated diabetes-induced elevations in renal levels of RAGE and phosphorylated NF-κB p65 subunit. It ameliorated glomerular injury due to diabetes by increasing glomerular nephrin and synaptopodin expressions, mitigating renal integrin-linked kinase (ILK) levels, and lowering urinary albumin, collagen type IV, and podocin excretions. FPS-ZM1 also improved renal function as demonstrated by decreasing levels of serum cystatin C. Additionally, the combination also alleviated indices of renal inflammation as revealed by decreased renal monocyte chemoattractant protein 1 (MCP-1) and chemokine (C-X-C motif) ligand 12 (CXCL12) expressions, F4/80-positive macrophages, glomerular TUNEL-positive cells, and urinary alpha-1-acid glycoprotein (AGP) levels. These findings underline the benefits of FPS-ZM1 added to valsartan in alleviating renal glomerular injury evoked by diabetes in streptozotocin rats and suggest FPS-ZM1 as a new potential adjunct to the conventional renin-angiotensin blockade.
Asunto(s)
Benzamidas/uso terapéutico , Diabetes Mellitus Experimental/complicaciones , Nefropatías Diabéticas/prevención & control , Barrera de Filtración Glomerular/efectos de los fármacos , Receptor para Productos Finales de Glicación Avanzada/antagonistas & inhibidores , Insuficiencia Renal/prevención & control , Valsartán/uso terapéutico , Administración Oral , Bloqueadores del Receptor Tipo 1 de Angiotensina II/administración & dosificación , Bloqueadores del Receptor Tipo 1 de Angiotensina II/uso terapéutico , Animales , Benzamidas/administración & dosificación , Biomarcadores/sangre , Biomarcadores/metabolismo , Biomarcadores/orina , Nefropatías Diabéticas/metabolismo , Nefropatías Diabéticas/patología , Nefropatías Diabéticas/fisiopatología , Quimioterapia Combinada , Barrera de Filtración Glomerular/metabolismo , Barrera de Filtración Glomerular/patología , Barrera de Filtración Glomerular/fisiopatología , Inyecciones Intraperitoneales , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Macrófagos/patología , Masculino , Microscopía Fluorescente , Fosforilación/efectos de los fármacos , Podocitos/efectos de los fármacos , Podocitos/inmunología , Podocitos/metabolismo , Podocitos/patología , Procesamiento Proteico-Postraduccional/efectos de los fármacos , Distribución Aleatoria , Ratas Wistar , Receptor para Productos Finales de Glicación Avanzada/metabolismo , Insuficiencia Renal/complicaciones , Insuficiencia Renal/metabolismo , Insuficiencia Renal/fisiopatología , Factor de Transcripción ReIA/metabolismo , Valsartán/administración & dosificaciónRESUMEN
PURPOSE: Euterpe oleracea Mart. (açaí) seed extract (ASE), through its anti-hypertensive, antioxidant and anti-inflammatory properties, may be useful to treat or prevent human diseases. Several evidences suggest that oxidative stress and inflammation contribute to the pathogenesis of diabetic nephropathy; therefore, we tested the hypothesis that ASE (200 mg/kg-1day-1) prevents diabetes and hypertension-related oxidative stress and inflammation, attenuating renal injury. METHODS: Male rats with streptozotocin (STZ)-induced diabetes (D), and spontaneously hypertensive rats with STZ-induced diabetes (DH) were treated daily with tap water or ASE (D + ASE and DH + ASE, respectively) for 45 days. The control (C) and hypertensive (H) animals received water. RESULTS: The elevated serum levels of urea and creatinine in D and DH, and increased albumin excretion in HD were reduced by ASE. Total glomeruli number in D and DH, were increased by ASE that also reduced renal fibrosis in both groups by decreasing collagen IV and TGF-ß1 expression. ASE improved biomarkers of renal filtration barrier (podocin and nephrin) in D and DH groups and prevented the increased expression of caspase-3, IL-6, TNF-α and MCP-1 in both groups. ASE reduced oxidative damage markers (TBARS, carbonyl levels and 8-isoprostane) in D and DH associated with a decrease in Nox 4 and p47 subunit expression and increase in antioxidant enzyme activity in both groups (SOD, catalase and GPx). CONCLUSION: ASE substantially reduced renal injury and prevented renal dysfunction by reducing inflammation, oxidative stress and improving the renal filtration barrier, providing a nutritional resource for prevention of diabetic and hypertensive-related nephropathy.
Asunto(s)
Antioxidantes/uso terapéutico , Nefropatías Diabéticas/prevención & control , Suplementos Dietéticos , Euterpe/química , Extractos Vegetales/uso terapéutico , Insuficiencia Renal/prevención & control , Semillas/química , Animales , Antiinflamatorios no Esteroideos/uso terapéutico , Antihipertensivos/uso terapéutico , Apoptosis , Biomarcadores/sangre , Biomarcadores/metabolismo , Biomarcadores/orina , Diabetes Mellitus Experimental/complicaciones , Diabetes Mellitus Experimental/dietoterapia , Diabetes Mellitus Experimental/inmunología , Nefropatías Diabéticas/complicaciones , Nefropatías Diabéticas/metabolismo , Nefropatías Diabéticas/patología , Fibrosis , Barrera de Filtración Glomerular/inmunología , Barrera de Filtración Glomerular/metabolismo , Barrera de Filtración Glomerular/patología , Barrera de Filtración Glomerular/fisiopatología , Hipertensión/complicaciones , Hipertensión/dietoterapia , Hipertensión/inmunología , Hipertensión/fisiopatología , Mediadores de Inflamación/sangre , Mediadores de Inflamación/metabolismo , Riñón/inmunología , Riñón/metabolismo , Riñón/patología , Riñón/fisiopatología , Estrés Oxidativo , Ratas Endogámicas SHR , Insuficiencia Renal/complicaciones , Insuficiencia Renal/etiología , Insuficiencia Renal/metabolismoRESUMEN
The kidney filtration barrier consists of three well-defined anatomic layers comprising a fenestrated endothelium, the glomerular basement membrane (GBM) and glomerular epithelial cells, the podocytes. Podocytes are post-mitotic and terminally differentiated cells with primary and secondary processes. The latter are connected by a unique cell-cell contact, the slit diaphragm. Podocytes maintain the GBM and seal the kidney filtration barrier to prevent the onset of proteinuria. Loss of prohibitin-1/2 (PHB1/2) in podocytes results not only in a disturbed mitochondrial structure but also in an increased insulin/IGF-1 signaling leading to mTOR activation and a detrimental metabolic switch. As a consequence, PHB-knockout podocytes develop proteinuria and glomerulosclerosis and eventually loss of renal function. In addition, experimental evidence suggests that PHB1/2 confer additional, extra-mitochondrial functions in podocytes as they localize to the slit diaphragm and thereby stabilize the unique intercellular contact between podocytes required to maintain an effective filtration barrier.
Asunto(s)
Metabolismo Energético , Barrera de Filtración Glomerular/metabolismo , Tasa de Filtración Glomerular , Mitocondrias/metabolismo , Proteínas Represoras/metabolismo , Transducción de Señal , Animales , Barrera de Filtración Glomerular/patología , Barrera de Filtración Glomerular/fisiopatología , Humanos , Enfermedades Renales/metabolismo , Enfermedades Renales/patología , Enfermedades Renales/fisiopatología , Mitocondrias/patología , Podocitos/metabolismo , Podocitos/patología , ProhibitinasRESUMEN
Glycogen storage disease type I (GSDI) is a rare metabolic disease due to glucose-6 phosphatase deficiency, characterized by fasting hypoglycemia. Patients also develop chronic kidney disease whose mechanisms are poorly understood. To decipher the process, we generated mice with a kidney-specific knockout of glucose-6 phosphatase (K.G6pc-/- mice) that exhibited the first signs of GSDI nephropathy after 6 months of G6pc deletion. We studied the natural course of renal deterioration in K.G6pc-/- mice for 18 months and observed the progressive deterioration of renal functions characterized by early tubular dysfunction and a later destruction of the glomerular filtration barrier. After 15 months, K.G6pc-/- mice developed tubular-glomerular fibrosis and podocyte injury, leading to the development of cysts and renal failure. On the basis of these findings, we were able to detect the development of cysts in 7 out of 32 GSDI patients, who developed advanced renal impairment. Of these 7 patients, 3 developed renal failure. In addition, no renal cysts were detected in six patients who showed early renal impairment. In conclusion, renal pathology in GSDI is characterized by progressive tubular dysfunction and the development of polycystic kidneys that probably leads to the development of irreversible renal failure in the late stages. Systematic observations of cyst development by kidney imaging should improve the evaluation of the disease's progression, independently of biochemical markers.
Asunto(s)
Barrera de Filtración Glomerular/patología , Glucosa-6-Fosfatasa/genética , Enfermedad del Almacenamiento de Glucógeno Tipo I/complicaciones , Enfermedades Renales Quísticas/etiología , Insuficiencia Renal/etiología , Adolescente , Adulto , Animales , Niño , Preescolar , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Femenino , Técnicas de Inactivación de Genes , Barrera de Filtración Glomerular/fisiopatología , Enfermedad del Almacenamiento de Glucógeno Tipo I/genética , Enfermedad del Almacenamiento de Glucógeno Tipo I/fisiopatología , Humanos , Lactante , Enfermedades Renales Quísticas/patología , Masculino , Ratones , Persona de Mediana Edad , Insuficiencia Renal/patología , Adulto JovenRESUMEN
OBJECTIVE: Revascularization of aortorenal bypass is a preferred technique for renal artery stenosis (RAS) in diabetic nephropathy (DN) patients. Restenosis of graft vessels also should be considered in patients lacking good control of blood glucose. In this study, we explored a combined strategy to prevent the recurrence of RAS in the DN rat model. METHODS: A model of DN was established by intraperitoneal injection of streptozotocin. Rats were divided into 4 groups: SR group, MIT group, Com group, and the untreated group. The levels of blood glucose and urine protein were measured, and changes in renal pathology were observed. The expression of monocyte chemoattractant protein-1 (MCP-1) in graft vessels was assessed by immunohistochemical staining. Histopathological staining was performed to assess the pathological changes of glomeruli and tubules. RESULTS: The levels of urine protein and the expression of MCP-1 in graft vessels were decreased after islet transplantation. The injury of glomerular basement membrane and podocytes was significantly ameliorated. CONCLUSIONS: The combined strategy of revascularization and microencapsulated islet transplantation had multiple protective effects on diabetic nephropathy, including preventing atherosclerosis in the graft vessels and alleviating injury to the glomerular filtration barrier. This combined strategy may be helpful for DN patients with RAS.
Asunto(s)
Aorta Abdominal/cirugía , Angiopatías Diabéticas/prevención & control , Nefropatías Diabéticas/cirugía , Trasplante de Islotes Pancreáticos , Obstrucción de la Arteria Renal/prevención & control , Arteria Renal/cirugía , Procedimientos Quirúrgicos Vasculares , Anastomosis Quirúrgica , Animales , Aorta Abdominal/metabolismo , Aorta Abdominal/patología , Biomarcadores/sangre , Glucemia/metabolismo , Quimiocina CCL2/metabolismo , Diabetes Mellitus Experimental/complicaciones , Angiopatías Diabéticas/sangre , Angiopatías Diabéticas/etiología , Nefropatías Diabéticas/sangre , Nefropatías Diabéticas/etiología , Barrera de Filtración Glomerular/fisiopatología , Tasa de Filtración Glomerular , Masculino , Proteinuria/etiología , Proteinuria/fisiopatología , Proteinuria/cirugía , Ratas Sprague-Dawley , Arteria Renal/metabolismo , Arteria Renal/patología , Obstrucción de la Arteria Renal/sangre , Obstrucción de la Arteria Renal/etiología , Factores de TiempoRESUMEN
AIMS: Growing evidences suggest that acute hyperglycemia is strongly related to kidney injury. Our study aimed to investigate the effects of acute hyperglycemia on kidney glomerular and tubular impairment in non-diabetic conscious rats. METHODS: Non-diabetic conscious rats were randomly subjected to 6h of saline (control group) or high glucose (acute hyperglycemia group) infusion. Blood glucose was maintained at 16.0-18.0 mmol/L in acute hyperglycemia group. Renal structure and function alterations, systemic/renal inflammation and oxidative stress markers were assessed, and apoptosis markers of renal inherent cells were evaluated. RESULTS: Acute hyperglycemia caused significant injury to structure of glomerular filtration barrier, tubular epithelial cells and peritubular vascular endothelial cells. It increased urinary microalbumin (68.01 ± 27.09 µg/24h vs 33.81 ± 13.81 µg/24h , P=0.014), ß2-microglobulin, Cystatin C, urinary and serous neutrophil gelatinase-associated lipocalin levels (P < 0.05). Acute hyperglycemia decreased megalin and cubilin expression, activated systemic and renal oxidative stress as well as inflammation and promoted renal inherent cell apoptosis. CONCLUSIONS: Acute hyperglycemia causes significant injury to kidney function and structure. Compared with damages of glomerular filtration barrier, renal tubular injury may contribute more to acute hyperglycemia induced proteinuria. Activation of inflammation especially renal inflammation, oxidative stress and enhanced apoptosis may be the underlying mechanisms.
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Apoptosis , Hiperglucemia/fisiopatología , Túbulos Renales/fisiopatología , Estrés Oxidativo , Insuficiencia Renal/etiología , Animales , Biomarcadores/sangre , Biomarcadores/metabolismo , Biomarcadores/orina , Glucemia/análisis , Barrera de Filtración Glomerular/inmunología , Barrera de Filtración Glomerular/metabolismo , Barrera de Filtración Glomerular/fisiopatología , Barrera de Filtración Glomerular/ultraestructura , Técnica de Clampeo de la Glucosa , Hiperglucemia/inmunología , Hiperglucemia/metabolismo , Hiperglucemia/patología , Glomérulos Renales/inmunología , Glomérulos Renales/metabolismo , Glomérulos Renales/fisiopatología , Glomérulos Renales/ultraestructura , Túbulos Renales/inmunología , Túbulos Renales/metabolismo , Túbulos Renales/ultraestructura , Masculino , Microscopía Electrónica de Transmisión , Nefritis/etiología , Especificidad de Órganos , Proteinuria/etiología , Distribución Aleatoria , Ratas Sprague-Dawley , Insuficiencia Renal/fisiopatología , Índice de Severidad de la EnfermedadRESUMEN
On the basis of observational studies, the most common cause of nephrotic syndrome in school-aged children is minimal change disease. On the basis of research evidence and consensus, corticosteroids are considered first-line therapy for treatment of nephrotic syndrome. On the basis of consensus, prednisone therapy should be initiated at doses of 60 mg/m2 per day (2 mg/kg per day) administered for 4 to 6 weeks, followed by 40 mg/m2 per dose (1.5 mg/kg) every other day for at least 6 to 8 weeks. On the basis of consensus and expert opinion, it is important to recognize and manage the complications that can arise in patients with nephrotic syndrome, such as dyslipidemia, infection, and thrombosis. On the basis of research evidence, consensus, and expert opinion, several alternative therapies have been observed to have variable efficacy in children with both corticosteroid-dependent and corticosteroid-resistant nephrotic syndrome, although caution must be exercised in the administration of these corticosteroid-sparing medications secondary to toxic adverse effects. On the basis of observational studies, the course of nephrotic syndrome in most patients is that of relapse and remission.
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Glucocorticoides/administración & dosificación , Síndrome Nefrótico/diagnóstico , Síndrome Nefrótico/tratamiento farmacológico , Prednisona/administración & dosificación , Corticoesteroides/uso terapéutico , Niño , Preescolar , Manejo de la Enfermedad , Femenino , Barrera de Filtración Glomerular/fisiopatología , Humanos , Lactante , Riñón/fisiopatología , Masculino , Síndrome Nefrótico/etiología , Pediatría/educaciónRESUMEN
AIMS: To evaluate the glomerular haemodynamic profile of patients with Type 1 diabetes with either renal hyperfiltration (GFR ≥ 135 ml/min/1.73 m2 ) or renal normofiltration (GFR 90-134 ml/min/1.73 m2 ) during euglycaemic and hyperglycaemic conditions, and to compare this profile with that of a similar group of healthy control subjects. METHODS: Gomez's equations were used to derive afferent and efferent arteriolar resistances, glomerular hydrostatic pressure and filtration pressure. RESULTS: At baseline, during clamped euglycaemia, patients with Type 1 diabetes and hyperfiltration had lower mean ± sd afferent arteriolar resistance than both those with Type 1 diabetes and normofiltration (914 ± 494 vs. 2065 ± 597 dyne/s/cm5 ; P < 0.001) and healthy control subjects (1676 ± 707 dyne/s/cm(5) ; p < 0.001). By contrast, efferent arteriolar resistance was similar in the three groups. Patients with Type 1 diabetes and hyperfiltration also had higher mean ± sd glomerular hydrostatic pressure than both healthy control subjects and patients with Type 1 diabetes and normofiltration (66 ± 6 vs. 60 ± 3 vs. 55 ± 3 mmHg; P < 0.05). Similar findings for afferent arteriolar resistance, efferent arteriolar resistance, glomerular hydrostatic pressure and filtration pressure were observed during clamped hyperglycaemia. CONCLUSION: Hyperfiltration in Type 1 diabetes is primarily driven by alterations in afferent arteriolar resistance rather than efferent arteriolar resistance. Renal protective therapies should focus on afferent renal arteriolar mechanisms through the use of pharmacological agents that target tubuloglomerular feedback, including sodium-glucose cotransporter 2 inhibitors and incretins.
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Diabetes Mellitus Tipo 1/complicaciones , Nefropatías Diabéticas/fisiopatología , Glomerulonefritis/complicaciones , Hiperglucemia/fisiopatología , Glomérulos Renales/fisiopatología , Circulación Renal , Adulto , Vías Aferentes/fisiopatología , Algoritmos , Arteriolas/inervación , Arteriolas/fisiopatología , Estudios de Cohortes , Diabetes Mellitus Tipo 1/sangre , Vías Eferentes/fisiopatología , Femenino , Barrera de Filtración Glomerular/irrigación sanguínea , Barrera de Filtración Glomerular/inervación , Barrera de Filtración Glomerular/fisiopatología , Tasa de Filtración Glomerular , Glomerulonefritis/fisiopatología , Técnica de Clampeo de la Glucosa , Humanos , Hiperglucemia/prevención & control , Glomérulos Renales/irrigación sanguínea , Glomérulos Renales/inervación , Masculino , Resistencia Vascular , Adulto JovenRESUMEN
Renal functional reserve (RFR) represents the capacity of the kidney to increase glomerular filtration rate (GFR) in response to certain physiological or pathological stimuli or conditions. Once baseline GFR is determined, RFR can be assessed clinically after an oral protein load or intravenous amino acid infusion. In clinical practice, baseline GFR displays variable levels due to diet or other factors. RFR is the difference between peak 'stress' GFR induced by the test (p.o. or i.v.) and the baseline GFR. In clinical scenarios where hyperfiltration is present (high baseline GFR due to pregnancy, hypertension or diabetic nephropathy, in solitary kidney or kidney donors), RFR may be fully or partially used to achieve normal or supranormal renal function. Since commonly used renal function markers, such as GFR, may remain within normal ranges until 50% of nephrons are lost or in patients with a single remnant kidney, the RFR test may represent a sensitive and early way to assess the functional decline in the kidney. RFR assessment may become an important tool to evaluate the ability of the kidney to recover completely or partially after a kidney attack. In case of healing with a defect and progressive fibrosis, recovery may appear complete clinically, but a reduced RFR may be a sign of a maladaptive repair or subclinical loss of renal mass. Thus, a reduction in RFR may represent the equivalent of renal frailty or susceptibility to insults. The main aim of this article is to review the concept of RFR, its utility in different clinical scenarios, and future perspective for its use.
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Lesión Renal Aguda/fisiopatología , Pruebas de Función Renal/métodos , Riñón/fisiopatología , Lesión Renal Aguda/complicaciones , Lesión Renal Aguda/terapia , Factores de Edad , Síndrome Cardiorrenal/fisiopatología , Sistema Cardiovascular/fisiopatología , Comorbilidad , Creatinina/sangre , Proteínas en la Dieta , Femenino , Barrera de Filtración Glomerular/fisiopatología , Tasa de Filtración Glomerular , Humanos , Donadores Vivos , Masculino , Embarazo , Complicaciones del Embarazo/fisiopatología , Recuperación de la Función , Insuficiencia Renal Crónica/etiología , Insuficiencia Renal Crónica/fisiopatología , Estrés Fisiológico , Obtención de Tejidos y Órganos/normasRESUMEN
BACKGROUND: It is well known that hypertension may cause glomerular damage, but the molecular mechanisms involved are still incompletely understood. METHODS: In the present study, we used formalin-fixed paraffin-embedded (FFPE) tissue to investigate changes in the glomerular proteome in the non-clipped kidney of two-kidney one-clip (2K1C) hypertensive rats, with special emphasis on the glomerular filtration barrier. 2K1C hypertension was induced in 6-week-old Wistar Hannover rats (n = 6) that were sacrificed 23 weeks later and compared with age-matched sham-operated controls (n = 6). Tissue was stored in FFPE tissue blocks and later prepared on tissue slides for laser microdissection. Glomeruli without severe morphological damage were isolated, and the proteomes were analysed using liquid chromatography-tandem mass spectrometry. RESULTS: 2K1C glomeruli showed reduced abundance of proteins important for slit diaphragm complex, such as nephrin, podocin and neph1. The podocyte foot process had a pattern of reduced abundance of transmembrane proteins but unchanged abundances of the podocyte cytoskeletal proteins synaptopodin and α-actinin-4. Lower abundance of important glomerular basement membrane proteins was seen. Possible glomerular markers of damage with increased abundance in 2K1C were transgelin, desmin and acyl-coenzyme A thioesterase 1. CONCLUSIONS: Microdissection and tandem mass spectrometry could be used to investigate the proteome of isolated glomeruli from FFPE tissue. Glomerular filtration barrier proteins had reduced abundance in the non-clipped kidney of 2K1C hypertensive rats.
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Barrera de Filtración Glomerular/fisiopatología , Hipertensión/metabolismo , Enfermedades Renales/metabolismo , Glomérulos Renales/metabolismo , Proteínas/metabolismo , Proteoma/metabolismo , Proteómica/métodos , Animales , Presión Sanguínea , Cromatografía Liquida , Modelos Animales de Enfermedad , Formaldehído , Hipertensión/patología , Hipertensión/fisiopatología , Enfermedades Renales/patología , Glomérulos Renales/patología , Masculino , Parafina , Ratas , Ratas Wistar , Espectrometría de Masas en TándemRESUMEN
BACKGROUND: Renal abnormalities are often seen in sickle cell disease (SCD). OBJECTIVE: To investigate the role of hydroxycarbamide as a protective agent in sickle cell nephropathy. SETTING: Patients with SCD followed at a Hematology outpatients clinic. METHODS: Prospective study with 26 SCD patients. Renal function evaluation was performed and a comparison between patients and control group was done. Patients using hydroxycarbamide were compared to those not taking this drug. MAIN OUTCOME MEASURE: Effect of hydroxycarbamide on renal function. RESULTS: Patients mean age was 32.1 ± 9.9 years, and 16 (61 %) were males. Glomerular hyperfiltration was found in nine patients with SCD (34.6 %). GFR < 60 mL/min/1.73 m² was observed in three cases (11.5 %). Microalbuminuria (30-300 mg/day) was found in seven cases (27 %) and macroalbuminuria (>300 mg/dia) in one patient (3.8 %). All patients had urinary concentrating deficit, and inability to acidify urine was found in ten cases (38.4 %). The comparison of patients according to the use of hydroxycarbamide showed lower levels of serum creatinine in those using the drug (0.6 ± 0.1 vs. 0.8 ± 0.3 mg/dL, p = 0.03), as well as lower levels of 24 h-proteinuria (226 ± 16 vs. 414 ± 76 mg/dL, p = 0.0001), but not microalbuminuria (79 ± 15 vs. 55 ± 86 mg/dL, p = 0.35). CONCLUSION: SCD is associated with important renal abnormalities. Hydroxycarbamide seems to protect kidney function in SCD by decreasing proteinuria but not microalbuminuria.