RESUMEN
Olfactory oscillations may enhance cognitive processing through coupling with beta (ß, 15-30 Hz) and gamma (γ, 30-160 Hz) activity in the hippocampus (HPC). We hypothesize that coupling between olfactory bulb (OB) and HPC oscillations is increased by cholinergic activation in control rats and is reduced in kainic-acid-treated epileptic rats, a model of temporal lobe epilepsy. OB γ2 (63-100 Hz) power was higher during walking and immobility-awake (IMM) compared to sleep, while γ1 (30-57 Hz) power was higher during grooming than other behavioral states. Muscarinic cholinergic agonist pilocarpine (25 mg/kg ip) with peripheral muscarinic blockade increased OB power and OB-HPC coherence at ß and γ1 frequency bands. A similar effect was found after physostigmine (0.5 mg/kg ip) but not scopolamine (10 mg/kg ip). Pilocarpine increased bicoherence and cross-frequency coherence (CFC) between OB slow waves (SW, 1-5 Hz) and hippocampal ß, γ1 and γ2 waves, with stronger coherence at CA1 alveus and CA3c than CA1 stratum radiatum. Bicoherence further revealed a nonlinear interaction of ß waves in OB with ß waves at the CA1-alveus. Beta and γ1 waves in OB or HPC were segregated at one phase of the OB-SW, opposite to the phase of γ2 and γ3 (100-160 Hz) waves, suggesting independent temporal processing of ß/γ1 versus γ2/γ3 waves. At CA1 radiatum, kainic-acid-treated epileptic rats compared to control rats showed decreased theta power, theta-ß and theta-γ2 CFC during baseline walking, decreased CFC of HPC SW with γ2 and γ3 waves during baseline IMM, and decreased coupling of OB SW with ß and γ2 waves at CA1 alveus after pilocarpine. It is concluded that ß and γ waves in the OB and HPC are modulated by a slow respiratory rhythm, in a cholinergic and behavior-dependent manner, and OB-HPC functional connectivity at ß and γ frequencies may enhance cognitive functions.
Asunto(s)
Ritmo beta , Ritmo Gamma , Hipocampo , Bulbo Olfatorio , Pilocarpina , Animales , Ritmo Gamma/efectos de los fármacos , Ritmo Gamma/fisiología , Masculino , Bulbo Olfatorio/efectos de los fármacos , Bulbo Olfatorio/fisiopatología , Bulbo Olfatorio/fisiología , Hipocampo/efectos de los fármacos , Hipocampo/fisiopatología , Hipocampo/fisiología , Ratas , Pilocarpina/farmacología , Ritmo beta/efectos de los fármacos , Ritmo beta/fisiología , Ácido Kaínico/farmacología , Agonistas Muscarínicos/farmacología , Modelos Animales de Enfermedad , Epilepsia del Lóbulo Temporal/fisiopatología , Epilepsia del Lóbulo Temporal/inducido químicamente , Escopolamina/farmacología , Fisostigmina/farmacología , Conducta Animal/efectos de los fármacos , Conducta Animal/fisiología , Antagonistas Muscarínicos/farmacologíaRESUMEN
Despite its high prevalence, the determinants of smelling impairment in COVID-19 remain not fully understood. In this work, we aimed to examine the association between olfactory bulb volume and the clinical trajectory of COVID-19-related smelling impairment in a large-scale magnetic resonance imaging (MRI) analysis. Data of non-vaccinated COVID-19 convalescents recruited within the framework of the prospective Hamburg City Health Study COVID Program between March and December 2020 were analyzed. At baseline, 233 participants underwent MRI and neuropsychological testing as well as a structured questionnaire for olfactory function. Between March and April 2022, olfactory function was assessed at follow-up including quantitative olfactometric testing with Sniffin' Sticks. This study included 233 individuals recovered from mainly mild to moderate SARS-CoV-2 infections. Longitudinal assessment demonstrated a declining prevalence of self-reported olfactory dysfunction from 67.1% at acute infection, 21.0% at baseline examination and 17.5% at follow-up. Participants with post-acute self-reported olfactory dysfunction had a significantly lower olfactory bulb volume at baseline than normally smelling individuals. Olfactory bulb volume at baseline predicted olfactometric scores at follow-up. Performance in neuropsychological testing was not significantly associated with the olfactory bulb volume. Our work demonstrates an association of long-term self-reported smelling dysfunction and olfactory bulb integrity in a sample of individuals recovered from mainly mild to moderate COVID-19. Collectively, our results highlight olfactory bulb volume as a surrogate marker that may inform diagnosis and guide rehabilitation strategies in COVID-19.
Asunto(s)
COVID-19 , Imagen por Resonancia Magnética , Trastornos del Olfato , Bulbo Olfatorio , SARS-CoV-2 , Humanos , Bulbo Olfatorio/fisiopatología , Bulbo Olfatorio/patología , Bulbo Olfatorio/diagnóstico por imagen , COVID-19/fisiopatología , COVID-19/complicaciones , Masculino , Femenino , Persona de Mediana Edad , Trastornos del Olfato/etiología , Trastornos del Olfato/fisiopatología , Adulto , SARS-CoV-2/aislamiento & purificación , Anciano , Estudios Prospectivos , Pruebas Neuropsicológicas , Olfato/fisiologíaRESUMEN
The phenomenon of contagious itch, observed in both humans and rodents, remains a topic of ongoing debate concerning its modulators and underlying pathways. This study delves into the relationship between contagious itch and familiar olfactory cues, a non-visual factor contributing to this intriguing behavior. Our findings showed that contagious itch in observer mice occurs during physical interaction with the cagemate itch-demonstrator but not with a stranger demonstrator or in a non-physical encounter condition. Notably, itch-experienced observer mice displayed an increased contagious itch behavior, highlighting the relevance of itch-associated memory in this phenomenon. Furthermore, anosmic observer mice, whether itch-naïve or itch-experienced, displayed no contagious itch behavior. These results demonstrate that the familiar olfactory cues, specifically cagemate body odors, are required for contagious itch behaviors in mice. In line with these behavioral findings, our study reveals increased activity in brain regions associated with olfaction, emotion, and memory during contagious itch, including the olfactory bulb, the amygdala, the hypothalamus, and the hippocampus, with this activity diminished in anosmic mice. In conclusion, our study unveils the critical role of familiar olfactory cues in driving contagious itch in mice, shedding light on the interplay between social factors, sensory perception, and memory in this phenomenon.
Asunto(s)
Señales (Psicología) , Prurito , Olfato , Animales , Prurito/fisiopatología , Ratones , Olfato/fisiología , Masculino , Conducta Animal , Relaciones Interpersonales , Ratones Endogámicos C57BL , Odorantes , Bulbo Olfatorio/fisiopatología , Encéfalo/fisiopatologíaRESUMEN
Anxiety is among the most fundamental mammalian behaviors. Despite the physiological and pathological importance, its underlying neural mechanisms remain poorly understood. Here, we recorded the activity of olfactory bulb (OB) and medial prefrontal cortex (mPFC) of rats, which are critical structures to brain's emotional processing network, while exploring different anxiogenic environments. Our results show that presence in anxiogenic contexts increases the OB and mPFC regional theta activities. Also, these local activity changes are associated with enhanced OB-mPFC theta power- and phase-based functional connectivity as well as OB-to-mPFC information transfer. Interestingly, these effects are more prominent in the unsafe zones of the anxiogenic environments, compared to safer zones. This consistent trend of changes in diverse behavioral environments as well as local and long-range neural activity features suggest that the dynamics of OB-mPFC circuit theta oscillations might underlie different types of anxiety behaviors, with possible implications for anxiety disorders.
Asunto(s)
Ansiedad , Bulbo Olfatorio , Corteza Prefrontal , Ritmo Teta , Corteza Prefrontal/fisiología , Corteza Prefrontal/fisiopatología , Animales , Ansiedad/fisiopatología , Ritmo Teta/fisiología , Bulbo Olfatorio/fisiología , Bulbo Olfatorio/fisiopatología , Masculino , Ratas , Ratas Sprague-Dawley , Conducta Animal/fisiologíaRESUMEN
Parkinson's disease (PD) is a prevalent neurodegenerative disorder that affects 7-10 million individuals worldwide. A common early symptom of PD is olfactory dysfunction (OD), and more than 90% of PD patients suffer from OD. Recent studies have highlighted a high incidence of OD in patients with SARS-CoV-2 infection. This review investigates the potential convergence of OD in PD and COVID-19, particularly focusing on the mechanisms by which neuroinflammation contributes to OD and neurological events. Starting from our fundamental understanding of the olfactory bulb, we summarize the clinical features of OD and pathological features of the olfactory bulb from clinical cases and autopsy reports in PD patients. We then examine SARS-CoV-2-induced olfactory bulb neuropathology and OD and emphasize the SARS-CoV-2-induced neuroinflammatory cascades potentially leading to PD manifestations. By activating microglia and astrocytes, as well as facilitating the aggregation of α-synuclein, SARS-CoV-2 could contribute to the onset or exacerbation of PD. We also discuss the possible contributions of NF-κB, the NLRP3 inflammasome, and the JAK/STAT, p38 MAPK, TLR4, IL-6/JAK2/STAT3 and cGAS-STING signaling pathways. Although olfactory dysfunction in patients with COVID-19 may be reversible, it is challenging to restore OD in patients with PD. With the emergence of new SARS-CoV-2 variants and the recurrence of infections, we call for continued attention to the intersection between PD and SARS-CoV-2 infection, especially from the perspective of OD.
Asunto(s)
COVID-19 , Enfermedades Neuroinflamatorias , Trastornos del Olfato , Enfermedad de Parkinson , SARS-CoV-2 , Humanos , COVID-19/complicaciones , COVID-19/fisiopatología , Enfermedad de Parkinson/fisiopatología , Enfermedad de Parkinson/complicaciones , Enfermedades Neuroinflamatorias/etiología , Enfermedades Neuroinflamatorias/fisiopatología , Enfermedades Neuroinflamatorias/inmunología , Trastornos del Olfato/etiología , Trastornos del Olfato/fisiopatología , Trastornos del Olfato/virología , Bulbo Olfatorio/fisiopatología , Bulbo Olfatorio/virología , Bulbo Olfatorio/patologíaRESUMEN
Overweight induced by high-fat diet (HFD) represents one of the major health concerns in modern societies, which can cause lasting peripheral and central metabolic disorders in all age groups. Specifically, childhood obesity could lead to life-long impact on brain development and functioning. On the other hand, environmental enrichment (EE) has been demonstrated to be beneficial for learning and memory. Here, we explored the impact of high-fat diet on olfaction and organization of olfactory bulb cells in adolescent mice, and the effect of EE intervention thereon. Puberty mice (3-week-old) fed with HFD for 10 weeks exhibited poorer odor sensitivity and olfactory memory relative to controls consuming standard chows. The behavioral deficits were rescued in the HFD group with EE intervention. Neuroanatomically, parvalbumin (PV) interneurons in the olfactory bulb (OB) were reduced in the HFD-fed animals relative to control, while EE intervention also normalized this alteration. In contrast, cells expressing calbindin (CB), doublecortin (DCX) in the OB were not altered. Our findings suggest that PV interneurons may play a crucial role in mediating the HFD-induced olfactory deficit in adolescent mice, and can also serve a protective effect of EE against the functional deficit.
Asunto(s)
Dieta Alta en Grasa/efectos adversos , Ambiente , Interneuronas/metabolismo , Trastornos del Olfato/etiología , Trastornos del Olfato/terapia , Bulbo Olfatorio , Parvalbúminas/metabolismo , Factores de Edad , Animales , Conducta Animal/fisiología , Modelos Animales de Enfermedad , Ratones , Bulbo Olfatorio/citología , Bulbo Olfatorio/metabolismo , Bulbo Olfatorio/fisiopatologíaRESUMEN
COVID-19 is a public health emergency with cases increasing globally. Its clinical manifestations range from asymptomatic and acute respiratory disease to multiple organ dysfunction syndromes and effects of COVID-19 in the long term. Interestingly, regardless of variant, all COVID-19 share impairment of the sense of smell and taste. We would like to report, as far as we know, the first comprehensive neurophysiological evaluation of the long-term effects of SARS-CoV-2 on the olfactory system with potential-related neurological damage. The case report concerns a military doctor, with a monitored health history, infected in April 2020 by the first wave of the epidemic expansion while on military duty in Codogno (Milan). In this subject, we find the electrophysiological signal in the periphery, while its correlate is absent in the olfactory bulb region than in whole brain recordings. In agreement with this result is the lack of metabolic signs of brain activation under olfactory stimulation. Consequently, quantitative and qualitative diagnoses of anosmia were made by means of olfactometric tests. We strongly suggest a comprehensive series of olfactometric tests from the first sign of COVID-19 and subsequent patient assessments. In conclusion, electrophysiological and metabolic tests of olfactory function have made it possible to study the long-term effects and the establishment of neurological consequences.
Asunto(s)
Anosmia/fisiopatología , Anosmia/virología , COVID-19/complicaciones , Adulto , COVID-19/fisiopatología , Electrofisiología/métodos , Potenciales Evocados/fisiología , Humanos , Masculino , Bulbo Olfatorio/fisiopatología , Nervio Olfatorio/fisiopatología , SARS-CoV-2 , Umbral Sensorial/fisiología , Síndrome Post Agudo de COVID-19RESUMEN
Disrupted systemic copper (Cu) homeostasis underlies neurodegenerative diseases with early symptoms including olfactory dysfunction. This study investigated the impact of Cu dyshomeostasis on olfactory function, adult neurogenesis, and neurochemical balance. Models of Cu deficiency (CuD) and Cu overload (CuO) were established by feeding adult rats with Cu-restricted diets plus ip. injection of a Cu chelator (ammonium tetrathiomolybdate) and excess Cu, respectively. CuD reduced Cu levels in the olfactory bulb (OB), subventricular zone (SVZ), rostral migratory stream (RMS), and striatum, while CuO increased Cu levels in these areas. The buried pellet test revealed both CuD and CuO prolonged the latency to uncover food. CuD increased neural proliferation and stem cells in the SVZ and newly differentiated neurons in the OB, whereas CuO caused opposite alterations, suggesting a "switch"-type function of Cu in regulating adult neurogenesis. CuO increased GABA in the OB, while both CuD and CuO reduced DOPAC, HVA, 5-HT and the DA turnover rate in olfactory-associated brain regions. Altered mRNA expression of Cu transport and storage proteins in tested brain areas were observed under both conditions. Together, results support an association between systemic Cu dyshomeostasis and olfactory dysfunction. Specifically, altered adult neurogenesis along the SVZ-RMS-OB pathway and neurochemical imbalance could be the factors that may contribute to olfactory dysfunction.
Asunto(s)
Encéfalo/metabolismo , Encéfalo/fisiopatología , Cobre/metabolismo , Neurogénesis , Bulbo Olfatorio/fisiopatología , Animales , Transporte Biológico , Biomarcadores/metabolismo , Proliferación Celular , Ácido Glutámico/metabolismo , Homeostasis , Masculino , Células-Madre Neurales , Neuronas/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas Sprague-Dawley , Ácido gamma-Aminobutírico/metabolismoRESUMEN
Olfactory damage develops at the early stages of Alzheimer's disease (AD). While amyloid-ß (Aß) oligomers are shown to impair inhibitory circuits in the olfactory bulb (OB), its underlying mechanisms remain unclear. Here, we investigated the olfactory dysfunction due to impaired inhibitory transmission to mitral cells (MCs) of the OB in APP/PS1 mice. Using electrophysiological studies, we found that MCs exhibited increased spontaneous firing rates as early as 3 months, much before development of Aß deposits in the brain. Furthermore, the frequencies but not amplitudes of MC inhibitory postsynaptic currents decreased markedly, suggesting that presynaptic GABA release is impaired while postsynaptic GABAA receptor responses remain intact. Notably, muscimol, a GABAA receptor agonist, improved odor identification and discrimination behaviors in APP/PS1 mice, reduced MC basal firing activity, and rescued inhibitory circuits along with reducing the Aß burden in the OB. Our study links the presynaptic deficits of GABAergic transmission to olfactory dysfunction and subsequent AD development and implicates the therapeutic potential of maintaining local inhibitory microcircuits against early AD progression.
Asunto(s)
Agonistas de Receptores de GABA-A/farmacología , Agonistas de Receptores de GABA-A/uso terapéutico , Trastornos del Olfato/tratamiento farmacológico , Trastornos del Olfato/fisiopatología , Bulbo Olfatorio/patología , Bulbo Olfatorio/fisiopatología , Receptores de GABA-A/fisiología , Olfato/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Enfermedad de Alzheimer/complicaciones , Enfermedad de Alzheimer/tratamiento farmacológico , Péptidos beta-Amiloides/efectos adversos , Péptidos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/genética , Animales , Encéfalo/metabolismo , Ratones Transgénicos , Trastornos del Olfato/etiología , Bulbo Olfatorio/citología , Presenilina-1/genética , Factores de TiempoRESUMEN
The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of human COVID-19, not only causes flu-like symptoms and gut microbiome complications but a large number of infected individuals also experience a host of neurological symptoms including loss of smell and taste, seizures, difficulty concentrating, decreased alertness, and brain inflammation. Although SARS-CoV-2 infections are not more prevalent in Parkinson's disease patients, a higher mortality rate has been reported not only associated with older age and longer disease duration, but also through several mechanisms, such as interactions with the brain dopaminergic system and through systemic inflammatory responses. Indeed, a number of the neurological symptoms seen in COVID-19 patients, as well as the alterations in the gut microbiome, are also prevalent in patients with Parkinson's disease. Furthermore, biochemical pathways such as oxidative stress, inflammation, and protein aggregation have shared commonalities between Parkinson's disease and COVID-19 disease progression. In this review, we describe and compare the numerous similarities and intersections between neurodegeneration in Parkinson's disease and RNA viral infections, emphasizing the current SARS-CoV-2 global health crisis.
Asunto(s)
COVID-19/fisiopatología , Microbioma Gastrointestinal , Enfermedad de Parkinson/fisiopatología , SARS-CoV-2 , Anciano , COVID-19/complicaciones , COVID-19/mortalidad , Trastornos del Conocimiento/etiología , Citocinas/fisiología , Dieta , Progresión de la Enfermedad , Disbiosis/etiología , Disbiosis/fisiopatología , Humanos , Inflamación , Metales Pesados/toxicidad , Modelos Neurológicos , Degeneración Nerviosa , Bulbo Olfatorio/fisiopatología , Bulbo Olfatorio/virología , Estrés Oxidativo , Enfermedad de Parkinson/etiología , Guías de Práctica Clínica como Asunto , Agregación Patológica de Proteínas/etiología , Infecciones por Virus ARN/metabolismo , Infecciones por Virus ARN/fisiopatología , Especies Reactivas de Oxígeno/metabolismo , Trastornos de la Sensación/etiología , alfa-Sinucleína/metabolismoRESUMEN
BACKGROUND: Before the deposition of amyloid-beta plaques and the onset of learning memory deficits, patients with Alzheimer's disease (AD) experience olfactory dysfunction, typified by a reduced ability to detect, discriminate, and identify odors. Rodent models of AD, such as the Tg2576 and APP/PS1 mice, also display impaired olfaction, accompanied by aberrant in vivo or in vitro gamma rhythms in the olfactory pathway. However, the mechanistic relationships between the electrophysiological, biochemical and behavioral phenomena remain unclear. METHODS: To address the above issues in AD models, we conducted in vivo measurement of local field potential (LFP) with a combination of in vitro electro-olfactogram (EOG), whole-cell patch and field recordings to evaluate oscillatory and synaptic function and pharmacological regulation in the olfactory pathway, particularly in the olfactory bulb (OB). Levels of protein involved in excitation and inhibition of the OB were investigated by western blotting and fluorescence staining, while behavioral studies assessed olfaction and memory function. RESULTS: LFP measurements demonstrated an increase in gamma oscillations in the OB accompanied by altered olfactory behavior in both APP/PS1 and 3xTg mice at 3-5 months old, i.e. an age before the onset of plaque formation. Fewer olfactory sensory neurons (OSNs) and a reduced EOG contributed to a decrease in the excitatory responses of M/T cells, suggesting a decreased ability of M/T cells to trigger interneuron GABA release indicated by altered paired-pulse ratio (PPR), a presynaptic parameter. Postsynaptically, there was a compensatory increase in levels of GABAAR α1 and ß3 subunits and subsequent higher amplitude of inhibitory responses. Strikingly, the GABA uptake inhibitor tiagabine (TGB) ameliorated abnormal gamma oscillations and levels of GABAAR subunits, suggesting a potential therapeutic strategy for early AD symptoms. These findings reveal increased gamma oscillations in the OB as a core indicator prior to onset of AD and uncover mechanisms underlying aberrant gamma activity in the OB. CONCLUSIONS: This study suggests that the concomitant dysfunction of both olfactory behavior and gamma oscillations have important implications for early AD diagnosis: in particular, awareness of aberrant GABAergic signaling mechanisms might both aid diagnosis and suggest therapeutic strategies for olfactory damage in AD.
Asunto(s)
Enfermedad de Alzheimer/metabolismo , Neuronas GABAérgicas/metabolismo , Bulbo Olfatorio/metabolismo , Placa Amiloide/metabolismo , Péptidos beta-Amiloides/metabolismo , Animales , Modelos Animales de Enfermedad , Femenino , Ritmo Gamma/fisiología , Humanos , Masculino , Ratones , Bulbo Olfatorio/fisiopatología , Olfato/fisiologíaRESUMEN
BACKGROUND: Deficits in odor detection and discrimination are premature symptoms of Alzheimer's disease (AD) that correlate with pathological signs in the olfactory bulb (OB) and piriform cortex (PCx). Similar olfactory dysfunction has been characterized in AD transgenic mice that overproduce amyloid-ß peptide (Aß), which can be prevented by reducing Aß levels by immunological and pharmacological means, suggesting that olfactory dysfunction depends on Aß accumulation and Aß-driven alterations in the OB and/or PCx, as well as on their activation. However, this possibility needs further exploration. OBJECTIVE: To characterize the effects of Aß on OB and PCx excitability/coupling and on olfaction. METHODS: Aß oligomerized solution (containing oligomers, monomers, and protofibrils) or its vehicle were intracerebroventricularlly injected two weeks before OB and PCx excitability and synchrony were evaluated through field recordings in vivo and in brain slices. Synaptic transmission from the OB to the PCx was also evaluated in slices. Olfaction was assessed through the habituation/dishabituation test. RESULTS: Aß did not affect lateral olfactory tract transmission into the PCx but reduced odor habituation and cross-habituation. This olfactory dysfunction was related to a reduction of PCx and OB network activity power in vivo. Moreover, the coherence between PCx-OB activities was also reduced by Aß. Finally, Aß treatment exacerbated the 4-aminopyridine-induced excitation in the PCx in slices. CONCLUSION: Our results show that Aß-induced olfactory dysfunction involves a complex set of pathological changes at different levels of the olfactory pathway including alterations in PCx excitability and its coupling with the OB. These pathological changes might contribute to hyposmia in AD.
Asunto(s)
Péptidos beta-Amiloides/toxicidad , Trastornos del Olfato/inducido químicamente , Trastornos del Olfato/fisiopatología , Bulbo Olfatorio/fisiopatología , Vías Olfatorias/fisiopatología , Fragmentos de Péptidos/toxicidad , Corteza Piriforme/fisiopatología , Péptidos beta-Amiloides/administración & dosificación , Animales , Ratones , Microinyecciones/métodos , Bulbo Olfatorio/efectos de los fármacos , Vías Olfatorias/efectos de los fármacos , Técnicas de Cultivo de Órganos , Fragmentos de Péptidos/administración & dosificación , Corteza Piriforme/efectos de los fármacosRESUMEN
Can alterations in experience trigger different plastic modifications in neuronal structure and function, and if so, how do they integrate at the cellular level? To address this question, we interrogated circuitry in the mouse olfactory bulb responsible for the earliest steps in odor processing. We induced experience-dependent plasticity in mice of either sex by blocking one nostril for one day, a minimally invasive manipulation that leaves the sensory organ undamaged and is akin to the natural transient blockage suffered during common mild rhinal infections. We found that such brief sensory deprivation produced structural and functional plasticity in one highly specialized bulbar cell type: axon-bearing dopaminergic neurons in the glomerular layer. After 24 h naris occlusion, the axon initial segment (AIS) in bulbar dopaminergic neurons became significantly shorter, a structural modification that was also associated with a decrease in intrinsic excitability. These effects were specific to the AIS-positive dopaminergic subpopulation because no experience-dependent alterations in intrinsic excitability were observed in AIS-negative dopaminergic cells. Moreover, 24 h naris occlusion produced no structural changes at the AIS of bulbar excitatory neurons, mitral/tufted and external tufted cells, nor did it alter their intrinsic excitability. By targeting excitability in one specialized dopaminergic subpopulation, experience-dependent plasticity in early olfactory networks might act to fine-tune sensory processing in the face of continually fluctuating inputs.SIGNIFICANCE STATEMENT Sensory networks need to be plastic so they can adapt to changes in incoming stimuli. To see how cells in mouse olfactory circuits can change in response to sensory challenges, we blocked a nostril for just one day, a naturally relevant manipulation akin to the deprivation that occurs with a mild cold. We found that this brief deprivation induces forms of axonal and intrinsic functional plasticity in one specific olfactory bulb cell subtype: axon-bearing dopaminergic interneurons. In contrast, intrinsic properties of axon-lacking bulbar dopaminergic neurons and neighboring excitatory neurons remained unchanged. Within the same sensory circuits, specific cell types can therefore make distinct plastic changes in response to an ever-changing external landscape.
Asunto(s)
Segmento Inicial del Axón/patología , Neuronas Dopaminérgicas/patología , Plasticidad Neuronal/fisiología , Bulbo Olfatorio/fisiopatología , Privación Sensorial/fisiología , Animales , Segmento Inicial del Axón/fisiología , Neuronas Dopaminérgicas/fisiología , Femenino , Masculino , Ratones , Ratones Endogámicos C57BLRESUMEN
Dietary zinc deficiency may lead to olfactory deficits, whose mechanism remains largely elusive. Olfactory ensheathing cells (OECs), a type of glial cells that support the function and neurogenesis in the olfactory bulb (OB), may play a pivotal role in the maintenance of the olfactory system. In the present study, we established a rat model of dietary zinc deficiency and found that severe zinc deficiency, but not marginal zinc deficiency, caused significantly reduced food intake, growth retardation, and apparent olfactory deficit in growing rats. We showed that severe zinc deficiency resulted in the loss of OECs in the olfactory nerve layer (ONL) of the olfactory bulb. In addition, we revealed that the number of TUNEL-positive cells increased markedly in the region, suggesting an involvement of apoptotic cell death in zinc deficiency-induced loss of OECs. Moreover, we found that treatment with zinc chelator N,N,N'N',-tetrakis (2-pyridylmethyl)ethylenediamine (TPEN) triggered the apoptosis of in vitro-cultured primary OECs. The apoptosis of OECs was correlated with significantly elevated expression of p53. Importantly, TUNEL and CCK-8 assays both demonstrated that treatment with p53 antagonist pifithrin-α (PFT-α) markedly attenuated TPEN-induced OEC apoptosis. These findings implicated that p53-triggered apoptosis of OECs might play an integral role in zinc deficiency-induced olfactory malfunction.
Asunto(s)
Apoptosis , Neuroglía/metabolismo , Bulbo Olfatorio/metabolismo , Olfato , Zinc/deficiencia , Animales , Células Cultivadas , Quelantes/farmacología , Etilenodiaminas/farmacología , Femenino , Neuroglía/efectos de los fármacos , Bulbo Olfatorio/citología , Bulbo Olfatorio/fisiopatología , Ratas , Ratas Sprague-Dawley , Proteína p53 Supresora de Tumor/metabolismo , Zinc/metabolismoRESUMEN
Three mechanisms have been proposed to account for COVID-19 associated olfactory dysfunction; obstruction of the olfactory cleft; epithelial injury and infection of the sustentacular supporting cells, which are known to express ACE2, or injury to the olfactory bulb due to axonal transport through olfactory sensory neurones. The absence of ACE2 expression by olfactory sensory neurones has led to the neurotropic potential of COVID-19 to be discounted. While an accumulating body of evidence supports olfactory epithelial injury as an important mechanism, this does not account for all the features of olfactory dysfunction seen in COVID-19; for example the duration of loss in some patients, evidence of changes within the olfactory bulb on MRI imaging, identification of viral particles within the olfactory bulb in post-mortem specimens and the inverse association between severity of COVID-19 and the prevalence of olfactory loss. The recent identification of a second route of viral entry mediated by NRP1 addresses many of these inconsistencies. Expression by the olfactory sensory neurones and their progenitor cells may facilitate direct injury and axonal transport to the olfactory bulb as well as a mechanism for delayed or absent recovery. Expression by regulatory T cells may play a central role in the cytokine storm. Variability in expression by age, race or gender may explain differing morbidity of infection and inverse association between anosmia and severity; in the case of higher expression there may be a higher risk of olfactory function but greater activation of regulatory T cells that may suppress the cytokine storm.
Asunto(s)
Enzima Convertidora de Angiotensina 2/fisiología , COVID-19/complicaciones , COVID-19/fisiopatología , Modelos Biológicos , Neuropilina-1/fisiología , Trastornos del Olfato/etiología , Trastornos del Olfato/fisiopatología , SARS-CoV-2 , Anosmia/etiología , Anosmia/fisiopatología , COVID-19/virología , Humanos , Imagen por Resonancia Magnética , Trastornos del Olfato/virología , Bulbo Olfatorio/diagnóstico por imagen , Bulbo Olfatorio/fisiopatología , Mucosa Olfatoria/lesiones , Mucosa Olfatoria/fisiopatología , Mucosa Olfatoria/virología , Neuronas Receptoras Olfatorias/fisiología , SARS-CoV-2/patogenicidad , Índice de Severidad de la Enfermedad , Olfato/fisiología , Linfocitos T Reguladores/inmunología , Internalización del VirusRESUMEN
Melatonin MT1 and MT2 receptors are expressed in the glomerular layer of the olfactory bulb (OB); however, the role of these receptors has not been evaluated until now. Considering the association of the OB with olfactory and depressive disorders in Parkinson's disease (PD), we sought to investigate the involvement of melatonin receptors in these non-motor disturbances in an intranigral 6-hydroxydopamine (6-OHDA)-lesioned rat model of PD. We demonstrate the presence of functional melatonin receptors in dopaminergic neurons of the glomerular layer. Local administration of melatonin (MLT, 1 µg/µl), luzindole (LUZ, 5 µg/µl) or the MT2-selective receptor drug 4-P-PDOT (5 µg/µl) reversed the depressive-like behavior elicited by 6-OHDA. Sequential administration of 4-P-PDOT and MLT (5 µg/µl, 1 µg/µl) promoted additive antidepressant-like effects. In the evaluation of olfactory discrimination, LUZ induced an olfactory impairment when associated with the nigral lesion-induced impairment. Thus, our results suggest that melatonin MT2 receptors expressed in the glomerular layer are involved in depressive-like behaviors and in olfactory function associated with PD.
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Anosmia/metabolismo , Conducta Animal , Trastorno Depresivo/metabolismo , Neuronas Dopaminérgicas/metabolismo , Bulbo Olfatorio/metabolismo , Trastornos Parkinsonianos/metabolismo , Receptor de Melatonina MT2/metabolismo , Animales , Anosmia/etiología , Anosmia/fisiopatología , Anosmia/psicología , Conducta Animal/efectos de los fármacos , Trastorno Depresivo/etiología , Trastorno Depresivo/fisiopatología , Trastorno Depresivo/psicología , Modelos Animales de Enfermedad , Neuronas Dopaminérgicas/efectos de los fármacos , Locomoción/efectos de los fármacos , Masculino , Melatonina/farmacología , Bulbo Olfatorio/efectos de los fármacos , Bulbo Olfatorio/fisiopatología , Percepción Olfatoria/efectos de los fármacos , Oxidopamina , Trastornos Parkinsonianos/inducido químicamente , Trastornos Parkinsonianos/fisiopatología , Trastornos Parkinsonianos/psicología , Ratas Wistar , Receptor de Melatonina MT2/efectos de los fármacos , Transducción de Señal , Olfato/efectos de los fármacos , Natación , Tetrahidronaftalenos/farmacología , Triptaminas/farmacologíaRESUMEN
To achieve visual space constancy, our brain remaps eye-centered projections of visual objects across saccades. Here, we measured saccade trajectory curvature following the presentation of visual, auditory, and audiovisual distractors in a double-step saccade task to investigate if this stability mechanism also accounts for localized sounds. We found that saccade trajectories systematically curved away from the position at which either a light or a sound was presented, suggesting that both modalities are represented in eye-centered oculomotor centers. Importantly, the same effect was observed when the distractor preceded the execution of the first saccade. These results suggest that oculomotor centers keep track of visual, auditory and audiovisual objects by remapping their eye-centered representations across saccades. Furthermore, they argue for the existence of a supra-modal map which keeps track of multi-sensory object locations across our movements to create an impression of space constancy.
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Movimientos Sacádicos/fisiología , Adulto , Femenino , Humanos , Masculino , Bulbo Olfatorio/fisiopatología , Percepción Espacial/fisiología , Adulto JovenRESUMEN
Failure of extinction of fear-conditioned traumatic memory is the main pathology behind post-traumatic stress disorder (PTSD). Functional and structural dysfunctions in the olfactory system are implicated by studies in PTSD patients. However, little is known regarding the neurobiological networks of traumarelated odor sensitivity in PTSD. Male Wistar rats were exposed with a female cat for 10 min and long-term stress was evaluated by behavioral tests, containing open field (OF) and elevated plus maze (EPM). To prove the PTSD model, the serum level of cortisol was evaluated and compared with the control group. Local field potential (LFP) was applied to compare the electrophysiology of the OB in two groups. To assess neurogenesis, the expression of nestin, and doublecortin were evaluated. Data from EPM revealed a significant increase in spent time in the closed arms in PTSD group. We observed a significant reduction in OF parameters in terms of the total distance traveled, the time spent in the central zone, and the number of crossing the central zone in PTSD group compared to the control group. The mean serum cortisol level was significantly higher in the PTSD group than the control group. In LFP recording, the slope and the amplitude of field excitatory postsynaptic potential (fEPSP) in the PTSD group were significantly higher than that of the control group. Our results also showed that the mRNA expression level of nestin as a neural progenitor marker and doublecortin, as an immature neuron marker, significantly decreased in the PTSD group compared to the control group. This study has shown that PTSD can disrupt the OB function through decreasing neurogenesis. More information on PTSD and OB would help us to establish a greater degree of accuracy on this matter.
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Ansiedad/fisiopatología , Miedo/fisiología , Bulbo Olfatorio/fisiopatología , Trastornos por Estrés Postraumático/fisiopatología , Animales , Modelos Animales de Enfermedad , Proteína Doblecortina , Femenino , Masculino , Memoria/fisiología , Neurogénesis/fisiología , Ratas Wistar , Trastornos por Estrés Postraumático/patologíaRESUMEN
Persons with trisomy 21 (Down syndrome) present different phenotypes, including early neurodegeneration, which is prominent in the brain olfactory areas, and olfactory deficit. The use of in vivo techniques in animal models allows to characterize and follow up these slowly developing phenomena. We explored by means of magnetic resonance imaging the olfactory bulb of the Ts65Dn mouse, an established model of Down syndrome, searching for possible syndromerelated changes. In vivo imaging provided a first glimpse of the trisomic olfactory bulb as compared to euploid one. The olfactory bulb volume was smaller in trisomic mice, suggesting that changes in olfactory bulb may be apparent already in the young adult (2 to 8monthold) mice, which are amenable to followup in vivo. These findings lead the way to future work aimed at characterizing the Down syndromerelated development of morphological alterations in the olfactory bulb and relating them to changes in olfactory performance, which were detected in this mouse model.
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Síndrome de Down/patología , Síndrome de Down/fisiopatología , Bulbo Olfatorio/patología , Bulbo Olfatorio/fisiopatología , Fenotipo , Animales , Modelos Animales de Enfermedad , Síndrome de Down/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Enfermedades Neurodegenerativas/genética , Enfermedades Neurodegenerativas/patologíaRESUMEN
Amyotrophic lateral sclerosis (ALS) is a fatal disease characterized by progressive muscle paralysis due to the degeneration of upper and lower motor neurons. Recent studies point out an involvement of the non-motor axis during disease progression. Despite smell impairment being considered a potential non-motor finding in ALS, the pathobiochemistry at the olfactory level remains unknown. Here, we applied an olfactory quantitative proteotyping approach to analyze the magnitude of the olfactory bulb (OB) proteostatic imbalance in ALS subjects (n = 12) with respect to controls (n = 8). Around 3% of the quantified OB proteome was differentially expressed, pinpointing aberrant protein expression involved in vesicle-mediated transport, macroautophagy, axon development and gliogenesis in ALS subjects. The overproduction of olfactory marker protein (OMP) points out an imbalance in the olfactory signal transduction in ALS. Accompanying the specific overexpression of glial fibrillary acidic protein (GFAP) and Bcl-xL in the olfactory tract (OT), a tangled disruption of signaling routes was evidenced across the OB-OT axis in ALS. In particular, the OB survival signaling dynamics clearly differ between ALS and frontotemporal lobar degeneration (FTLD), two faces of TDP-43 proteinopathy. To the best of our knowledge, this is the first report on high-throughput molecular characterization of the olfactory proteostasis in ALS.