RESUMEN
In the mammalian cochlea, specialized ribbon-type synapses between sensory inner hair cells (IHCs) and postsynaptic spiral ganglion neurons ensure the temporal precision and indefatigability of synaptic sound encoding. These high-through-put synapses are presynaptically characterized by an electron-dense projection-the synaptic ribbon-which provides structural scaffolding and tethers a large pool of synaptic vesicles. While advances have been made in recent years in deciphering the molecular anatomy and function of these specialized active zones, the developmental assembly of this presynaptic interaction hub remains largely elusive. In this review, we discuss the dynamic nature of IHC (pre-) synaptogenesis and highlight molecular key players as well as the transport pathways underlying this process. Since developmental assembly appears to be a highly dynamic process, we further ask if this structural plasticity might be maintained into adulthood, how this may influence the functional properties of a given IHC synapse and how such plasticity could be regulated on the molecular level. To do so, we take a closer look at other ribbon-bearing systems, such as retinal photoreceptors and pinealocytes and aim to infer conserved mechanisms that may mediate these phenomena.
Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Células Ciliadas Auditivas Internas/metabolismo , Células Ciliadas Auditivas Externas/metabolismo , Sinapsis/metabolismo , Vesículas Sinápticas/metabolismo , Oxidorreductasas de Alcohol/genética , Oxidorreductasas de Alcohol/metabolismo , Animales , Proteínas Co-Represoras/genética , Proteínas Co-Represoras/metabolismo , Proteínas del Citoesqueleto/genética , Proteínas del Citoesqueleto/metabolismo , Citoesqueleto/metabolismo , Citoesqueleto/ultraestructura , Células Ciliadas Auditivas Internas/ultraestructura , Células Ciliadas Auditivas Externas/ultraestructura , Células Ciliadas Vestibulares/metabolismo , Células Ciliadas Vestibulares/ultraestructura , Mecanotransducción Celular , Ratones , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/metabolismo , Plasticidad Neuronal/genética , Neuropéptidos/genética , Neuropéptidos/metabolismo , Ratas , Sinapsis/ultraestructura , Transmisión Sináptica/genética , Vesículas Sinápticas/ultraestructuraRESUMEN
Sensory hair cells of the inner ear rely on the hair bundle, a cluster of actin-filled stereocilia, to transduce auditory and vestibular stimuli into electrical impulses. Because they are long and thin projections, stereocilia are most prone to damage at the point where they insert into the hair cell's soma. Moreover, this is the site of stereocilia pivoting, the mechanical movement that induces transduction, which additionally weakens this area mechanically. To bolster this fragile area, hair cells construct a dense core called the rootlet at the base of each stereocilium, which extends down into the actin meshwork of the cuticular plate and firmly anchors the stereocilium. Rootlets are constructed with tightly packed actin filaments that extend from stereocilia actin filaments which are wrapped with TRIOBP; in addition, many other proteins contribute to the rootlet and its associated structures. Rootlets allow stereocilia to sustain innumerable deflections over their lifetimes and exemplify the unique manner in which sensory hair cells exploit actin and its associated proteins to carry out the function of mechanotransduction.
Asunto(s)
Actinas/análisis , Células Ciliadas Auditivas/citología , Células Ciliadas Vestibulares/citología , Estereocilios/ultraestructura , Actinas/metabolismo , Animales , Células Ciliadas Auditivas/química , Células Ciliadas Auditivas/metabolismo , Células Ciliadas Auditivas/ultraestructura , Células Ciliadas Vestibulares/química , Células Ciliadas Vestibulares/metabolismo , Células Ciliadas Vestibulares/ultraestructura , Audición , Humanos , Mecanotransducción Celular , Equilibrio Postural , Estereocilios/química , Estereocilios/metabolismoRESUMEN
The auditory apparatus of the inner ear does not show turnover of sensory hair cells (HCs) in adult mammals; in contrast, there are many observations supporting low-level turnover of vestibular HCs within the balance organs of mammalian inner ears. This low-level renewal of vestibular HCs exists during normal conditions and it is further enhanced after trauma-induced loss of these HCs. The main process for renewal of HCs within mammalian vestibular epithelia is a conversion/transdifferentiation of existing supporting cells (SCs) into replacement HCs.In earlier studies using long-term organ cultures of postnatal rat macula utriculi, HC loss induced by gentamicin resulted in an initial substantial decline in HC density followed by a significant increase in the proportion of HCs to SCs indicating the production of replacement HCs. In the present study, using the same model of ototoxic damage to study renewal of vestibular HCs, we focus on the ultrastructural characteristics of SCs undergoing transdifferentiation into new HCs. Our objective was to search for morphological signs of SC plasticity during this process. In the utricular epithelia, we observed immature HCs, which appear to be SCs transdifferentiating into HCs. These bridge SCs have unique morphological features characterized by formation of foot processes, basal accumulation of mitochondria, and an increased amount of connections with nearby SCs. No gap junctions were observed on these transitional cells. The tight junction seals were morphologically intact in both control and gentamicin-exposed explants. Anat Rec, 303:506-515, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.
Asunto(s)
Transdiferenciación Celular/fisiología , Gentamicinas/toxicidad , Células Ciliadas Vestibulares/ultraestructura , Sáculo y Utrículo/ultraestructura , Células Madre/ultraestructura , Animales , Células Ciliadas Vestibulares/efectos de los fármacos , Ototoxicidad , Ratas , Ratas Wistar , Sáculo y Utrículo/efectos de los fármacos , Células Madre/efectos de los fármacosRESUMEN
Ribbon synapses of cochlear inner hair cells (IHCs) undergo molecular assembly and extensive functional and structural maturation before hearing onset. Here, we characterized the nanostructure of IHC synapses from late prenatal mouse embryo stages (embryonic days 14-18) into adulthood [postnatal day (P)48] using electron microscopy and tomography as well as optical nanoscopy of apical turn organs of Corti. We find that synaptic ribbon precursors arrive at presynaptic active zones (AZs) after afferent contacts have been established. These ribbon precursors contain the proteins RIBEYE and piccolino, tether synaptic vesicles and their delivery likely involves active, microtubule-based transport pathways. Synaptic contacts undergo a maturational transformation from multiple small to one single, large AZ. This maturation is characterized by the fusion of ribbon precursors with membrane-anchored ribbons that also appear to fuse with each other. Such fusion events are most frequently encountered around P12 and hence, coincide with hearing onset in mice. Thus, these events likely underlie the morphological and functional maturation of the AZ. Moreover, the postsynaptic densities appear to undergo a similar refinement alongside presynaptic maturation. Blockwise addition of ribbon material by fusion as found during AZ maturation might represent a general mechanism for modulating ribbon size.
Asunto(s)
Cóclea/crecimiento & desarrollo , Células Ciliadas Auditivas Internas/fisiología , Células Ciliadas Vestibulares/fisiología , Sinapsis/fisiología , Animales , Cóclea/ultraestructura , Células Ciliadas Auditivas Internas/ultraestructura , Células Ciliadas Vestibulares/ultraestructura , Audición/fisiología , Ratones/embriología , Microscopía Electrónica , Modelos Animales , Sinapsis/ultraestructura , Vesículas Sinápticas , TomografíaRESUMEN
High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.
Asunto(s)
Frío , Tomografía con Microscopio Electrónico/métodos , Células Ciliadas Vestibulares/ultraestructura , Estereocilios/ultraestructura , Animales , RatonesRESUMEN
Human vestibular sensory epithelia in explant culture were incubated in gentamicin to ablate hair cells. Subsequent transduction of supporting cells with ATOH1 using an Ad-2 viral vector resulted in generation of highly significant numbers of cells expressing the hair cell marker protein myosin VIIa. Cells expressing myosin VIIa were also generated after blocking the Notch signalling pathway with TAPI-1 but less efficiently. Transcriptomic analysis following ATOH1 transduction confirmed up-regulation of 335 putative hair cell marker genes, including several downstream targets of ATOH1. Morphological analysis revealed numerous cells bearing dense clusters of microvilli at the apical surfaces which showed some hair cell-like characteristics confirming a degree of conversion of supporting cells. However, no cells bore organised hair bundles and several expected hair cell markers genes were not expressed suggesting incomplete differentiation. Nevertheless, the results show a potential to induce conversion of supporting cells in the vestibular sensory tissues of humans.
Asunto(s)
Epitelio/fisiología , Células Ciliadas Vestibulares/fisiología , Regeneración/fisiología , Adenoviridae/metabolismo , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo , Epitelio/ultraestructura , Regulación de la Expresión Génica , Gentamicinas/efectos adversos , Proteínas Fluorescentes Verdes/metabolismo , Células Ciliadas Vestibulares/patología , Células Ciliadas Vestibulares/ultraestructura , Humanos , Miosina VIIa , Miosinas/metabolismo , Receptores Notch/metabolismo , Sáculo y Utrículo/fisiología , Sáculo y Utrículo/ultraestructura , Transducción de Señal , Transducción GenéticaRESUMEN
Sensory hair cells require control of physical properties of their apical plasma membranes for normal development and function. Members of the ADP-ribosylation factor (ARF) small GTPase family regulate membrane trafficking and cytoskeletal assembly in many cells. We identified ELMO domain-containing protein 1 (ELMOD1), a guanine nucleoside triphosphatase activating protein (GAP) for ARF6, as the most highly enriched ARF regulator in hair cells. To characterize ELMOD1 control of trafficking, we analyzed mice of both sexes from a strain lacking functional ELMOD1 [roundabout (rda)]. In rda/rda mice, cuticular plates of utricle hair cells initially formed normally, then degenerated after postnatal day 5; large numbers of vesicles invaded the compromised cuticular plate. Hair bundles initially developed normally, but the cell's apical membrane lifted away from the cuticular plate, and stereocilia elongated and fused. Membrane trafficking in type I hair cells, measured by FM1-43 dye labeling, was altered in rda/rda mice. Consistent with the proposed GAP role for ELMOD1, the ARF6 GTP/GDP ratio was significantly elevated in rda/rda utricles compared with controls, and the level of ARF6-GTP was correlated with the severity of the rda/rda phenotype. These results suggest that conversion of ARF6 to its GDP-bound form is necessary for final stabilization of the hair bundle.SIGNIFICANCE STATEMENT Assembly of the mechanically sensitive hair bundle of sensory hair cells requires growth and reorganization of apical actin and membrane structures. Hair bundles and apical membranes in mice with mutations in the Elmod1 gene degenerate after formation, suggesting that the ELMOD1 protein stabilizes these structures. We show that ELMOD1 is a GTPase-activating protein in hair cells for the small GTP-binding protein ARF6, known to participate in actin assembly and membrane trafficking. We propose that conversion of ARF6 into the GDP-bound form in the apical domain of hair cells is essential for stabilizing apical actin structures like the hair bundle and ensuring that the apical membrane forms appropriately around the stereocilia.
Asunto(s)
Factores de Ribosilacion-ADP/metabolismo , Proteínas Activadoras de GTPasa/metabolismo , Células Ciliadas Vestibulares/metabolismo , Factor 6 de Ribosilación del ADP , Animales , Femenino , Guanosina Trifosfato/metabolismo , Células Ciliadas Vestibulares/ultraestructura , Hidrólisis , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Transporte de Proteínas , Estereocilios/metabolismo , Estereocilios/ultraestructuraRESUMEN
Wound healing in the inner ear sensory epithelia is performed by the apical domains of supporting cells (SCs). Junctional F-actin belts of SCs are thin during development but become exceptionally thick during maturation. The functional significance of the thick belts is not fully understood. We have studied the role of F-actin belts during wound healing in the developing and adult cochlea of mice in vivo. We show that the thick belts serve as intracellular scaffolds that preserve the positions of surviving cells in the cochlear sensory epithelium. Junctions associated with the thick F-actin belts did not readily disassemble during wound healing. To compensate for this, basolateral membranes of SCs participated in the closure of surface breach. Because not only neighboring but also distant SCs contributed to wound healing by basolateral protrusions, this event appears to be triggered by contact-independent diffusible signals. In the search for regulators of wound healing, we inactivated RhoA in SCs, which, however, did not limit wound healing. RhoA inactivation in developing outer hair cells (OHCs) caused myosin II delocalization from the perijunctional domain and apical cell-surface enlargement. These abnormalities led to the extrusion of OHCs from the epithelium. These results demonstrate the importance of stability of the apical domain, both in wound repair by SCs and in development of OHCs, and that only this latter function is regulated by RhoA. Because the correct cytoarchitecture of the cochlear sensory epithelium is required for normal hearing, the stability of cell apices should be maintained in regenerative and protective interventions.
Asunto(s)
Cóclea , Citoesqueleto/metabolismo , Regulación del Desarrollo de la Expresión Génica/genética , Células Ciliadas Vestibulares/metabolismo , Cicatrización de Heridas/genética , Proteína de Unión al GTP rhoA/metabolismo , Actinas/metabolismo , Factores de Edad , Animales , Animales Recién Nacidos , Cóclea/citología , Cóclea/embriología , Cóclea/crecimiento & desarrollo , Embrión de Mamíferos , Epitelio/embriología , Epitelio/crecimiento & desarrollo , Epitelio/metabolismo , Epitelio/ultraestructura , Femenino , Células Ciliadas Vestibulares/ultraestructura , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Microscopía Electrónica de Rastreo , Miosina Tipo II/metabolismo , Embarazo , Receptor Tipo 3 de Factor de Crecimiento de Fibroblastos/genética , Receptor Tipo 3 de Factor de Crecimiento de Fibroblastos/metabolismo , Proteína de Unión al GTP rhoA/genéticaRESUMEN
The paratympanic organ (PTO) is a sensory organ located in the medial wall of the tympanic cavity of birds. The organ looks like a small tapering vesicle, and is equipped with a sensory epithelium formed by supporting cells (SCs) and Type II hair cells (Type II-HCs). The function of the PTO has not yet been precisely defined. The prevailing current hypothesis is that the PTO assesses the air pressure exerted on the external surface of the tympanic membrane. The PTO could may thus function as a barometer and, in flying birds, also as an altimeter. The afferent synapses of the PTO of chicken were described in detail in a previous paper. Reciprocal synapses between efferent nerve endings (ENEs) and the HCs were also observed, suggesting the existence of local microcircuits. The aim of this work was to provide a more detailed ultrastructural description of these microcircuits in the PTO of chicken. We observed for the first time: (1) reciprocal synapses between the HCs and the afferent nerve endings (ANEs); (2) presence of two distinct types of ENEs; (3) reciprocal synapses between the HCs and both types of ENEs. Overall, these results indicate that a complex processing of the incoming sensory signals may occur in the PTO. This thus suggests that the PTO may perform more complex functions than those supposed until now. We hypothesize that the PTO could have a role in the low-frequency sound perception.
Asunto(s)
Axones/ultraestructura , Oído Medio/ultraestructura , Células Ciliadas Vestibulares/ultraestructura , Ligamentos/ultraestructura , Estereocilios/ultraestructura , Sinapsis/ultraestructura , Presión del Aire , Animales , PollosRESUMEN
Stereocilia are actin-based protrusions on auditory and vestibular sensory cells that are required for hearing and balance. They convert physical force from sound, head movement or gravity into an electrical signal, a process that is called mechanoelectrical transduction. This function depends on the ability of sensory cells to grow stereocilia of defined lengths. These protrusions form a bundle with a highly precise geometry that is required to detect nanoscale movements encountered in the inner ear. Congenital or progressive stereocilia degeneration causes hearing loss. Thus, understanding stereocilia hair bundle structure, development, and maintenance is pivotal to understanding the pathogenesis of deafness. Stereocilia cores are made from a tightly packed array of parallel, crosslinked actin filaments, the length and stability of which are regulated in part by myosin motors, actin crosslinkers and capping proteins. This review aims to describe stereocilia actin regulation in the context of an emerging "tip turnover" model where actin assembles and disassembles at stereocilia tips while the remainder of the core is exceptionally stable.
Asunto(s)
Actinas/genética , Células Ciliadas Auditivas/ultraestructura , Células Ciliadas Vestibulares/ultraestructura , Audición/fisiología , Mecanotransducción Celular , Estereocilios/ultraestructura , Citoesqueleto de Actina/metabolismo , Citoesqueleto de Actina/ultraestructura , Actinas/metabolismo , Animales , Proteínas Relacionadas con las Cadherinas , Cadherinas/genética , Cadherinas/metabolismo , Sordera/patología , Sordera/fisiopatología , Expresión Génica , Células Ciliadas Auditivas/metabolismo , Células Ciliadas Vestibulares/metabolismo , Ratones , Modelos Biológicos , Morfogénesis , Miosinas/genética , Miosinas/metabolismo , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , Estabilidad Proteica , Estereocilios/metabolismoRESUMEN
The nitrile 3,3'-iminodipropionitrile (IDPN) causes a loss of hair cells in the vestibular epithelium of the inner ear in several species of both mammals and nonmammals. It is of interest as a model compound in ototoxicity and vestibular regeneration research, but its effects on the mouse, including the potential relevance of strain and sex differences for susceptibility, have not yet been thoroughly characterized. In this study, we compared the vestibular toxicity of IDPN in dose-response studies (0, 8, 12, 16, and 24 mmol/kg IDPN p.o.) in males and females of 2 different mouse strains (RjOrl:Swiss/CD-1 and 129S1/SvImJ). 3,3'-Iminodipropionitrile caused a dose-dependent loss of vestibular function in all sex and strain groups, as assessed by a specific battery of behavioral tests. However, large differences in systemic toxicity were recorded, with high systemic toxicity in 129S1 mice of both sexes compared to limited effects on the Swiss mice. Both male and female Swiss mice showed a marked increase of hindlimb stride width after exposure. The Swiss, but not the 129S1, mice treated with IDPN showed hyperactivity in the open field. The dose-response relationships in the behavioral effects were matched by the extent of hair cell loss assessed by scanning electron microscopy. Altogether, the data demonstrated prominent strain-dependent differences in the systemic toxicity of IDPN between 129S1 and Swiss mice, in contrast to no differences between the strains and small differences between the sexes in its vestibular toxicity. These results support the use of Swiss mice exposed to IDPN as a mouse lesion model for research in vestibular therapy and regeneration.
Asunto(s)
Acatisia Inducida por Medicamentos/patología , Vestibulopatía Bilateral/inducido químicamente , Resistencia a Medicamentos , Células Ciliadas Vestibulares/efectos de los fármacos , Nitrilos/toxicidad , Xenobióticos/toxicidad , Administración Oral , Acatisia Inducida por Medicamentos/fisiopatología , Animales , Animales no Consanguíneos , Conducta Animal/efectos de los fármacos , Vestibulopatía Bilateral/patología , Vestibulopatía Bilateral/fisiopatología , Relación Dosis-Respuesta a Droga , Conducta Exploratoria/efectos de los fármacos , Femenino , Marcha/efectos de los fármacos , Células Ciliadas Vestibulares/patología , Células Ciliadas Vestibulares/ultraestructura , Masculino , Ratones , Ratones de la Cepa 129 , Microscopía Electrónica de Rastreo , Nitrilos/administración & dosificación , Caracteres Sexuales , Especificidad de la Especie , Pruebas de Toxicidad Aguda , Pérdida de Peso/efectos de los fármacos , Xenobióticos/administración & dosificaciónRESUMEN
Damage to inner ear afferent terminals is believed to result in many auditory and vestibular dysfunctions. The sequence of afferent injuries and repair, as well as their correlation with vertigo symptoms, remains poorly documented. In particular, information on the changes that take place at the primary vestibular endings during the first hours following a selective insult is lacking. In the present study, we combined histological analysis with behavioral assessments of vestibular function in a rat model of unilateral vestibular excitotoxic insult. Excitotoxicity resulted in an immediate but transient alteration of the balance function that was resolved within a week. Concomitantly, vestibular primary afferents underwent a sequence of structural changes followed by spontaneous repair. Within the first two hours after the insult, a first phase of pronounced vestibular dysfunction coincided with extensive swelling of afferent terminals. In the next 24â h, a second phase of significant but incomplete reduction of the vestibular dysfunction was accompanied by a resorption of swollen terminals and fiber retraction. Eventually, within 1 week, a third phase of complete balance restoration occurred. The slow and progressive withdrawal of the balance dysfunction correlated with full reconstitution of nerve terminals. Competitive re-innervation by afferent and efferent terminals that mimicked developmental synaptogenesis resulted in full re-afferentation of the sensory epithelia. By deciphering the sequence of structural alterations that occur in the vestibule during selective excitotoxic impairment, this study offers new understanding of how a vestibular insult develops in the vestibule and how it governs the heterogeneity of vertigo symptoms.
Asunto(s)
Conducta Animal , Neuronas Aferentes/patología , Neurotoxinas/toxicidad , Vértigo/patología , Vestíbulo del Laberinto/inervación , Vestíbulo del Laberinto/patología , Animales , Recuento de Células , Modelos Animales de Enfermedad , Oído Medio/efectos de los fármacos , Oído Medio/patología , Epitelio/efectos de los fármacos , Epitelio/patología , Femenino , Células Ciliadas Vestibulares/patología , Células Ciliadas Vestibulares/ultraestructura , Inyecciones , Ácido Kaínico/administración & dosificación , Modelos Biológicos , Neuronas Aferentes/efectos de los fármacos , Ratas Wistar , Vesículas Sinápticas/efectos de los fármacos , Vesículas Sinápticas/metabolismo , Vesículas Sinápticas/ultraestructura , Sinaptofisina/metabolismo , Factores de Tiempo , Vestíbulo del Laberinto/ultraestructuraRESUMEN
Ototoxicity is known to cause permanent loss of vestibule function through degeneration of sensory hair cells (HCs). However, functional recovery has been reported during washout after chronic ototoxicity, although the mechanisms underlying this reversible dysfunction are unknown. Here, we study this question in rats chronically exposed to the ototoxic compound 3,3'-iminodipropionitrile (IDPN). Pronounced alterations in vestibular function appeared before significant loss of HCs or stereociliary coalescence became evident by ultrastructural analyses. This early dysfunction was fully reversible if the exposure was terminated promptly. In cristae and utricles, the distinct junctions formed between type I HCs (HCI) and calyx endings were completely dismantled at these early stages of reversible dysfunction, and completely rebuilt during washout. Immunohistochemical observations revealed loss and recovery of the junction proteins CASPR1 and tenascin-C and RT-PCR indicated that their loss was not due to decreased gene expression. KCNQ4 was mislocalized during intoxication and recovered control-like localization after washout. At early stages of the intoxication, the calyces could be classified as showing intact or lost junctions, indicating that calyceal junction dismantlement is triggered on a calyx-by-calyx basis. Chronic toxicity also altered the presence of ribeye, PSD-95 and GluA2 puncta in the calyces. These synaptic alterations varied between the two types of calyx endings (formed by calyx-only or dimorphic afferents) and some persisted at the end of the washout period. The present data reveal new forms of plasticity of the calyx endings in adult mammals, including a robust capacity for rebuilding the calyceal junction. These findings contribute to a better understanding of the phenomena involved in progressive vestibular dysfunction and its potential recovery during and after ototoxic exposure.
Asunto(s)
Células Ciliadas Vestibulares/patología , Sinapsis/metabolismo , Animales , Enfermedad Crónica , Epitelio/metabolismo , Epitelio/patología , Epitelio/ultraestructura , Células Ciliadas Vestibulares/ultraestructura , Canales de Potasio KCNQ/metabolismo , Masculino , Nitrilos , Ratas Long-Evans , Estereocilios/patología , Tenascina/metabolismoRESUMEN
A medicina regenerativa implica em uma mudança de paradigma, a regeneração do organismo ao nível celular ou tecidual – um assunto contemporâneo controverso e de difícil estandardização. O artigo apresenta um resumo das tendências científicas, econômicas, sociais e de regulamentação global nessa área, analisadas em relação a dilemas teóricos relevantes em antropologia médica e sociologia da ciência e da saúde. Em especial, aqueles que tratam da construção de um ‘aparato coletivo de sentido’ para as novas entidades biológicas e ontológicas, a formação da cidadania biológica e a governança pela incerteza. Apresentam-se, também, evidências empíricas sobre um fenômeno chave para a governança e a regulamentação, qual seja a instalação de uma nova demanda transnacional em pesquisa e saúde através de mercados paralelos de óvulos e de terapias celulares em experimentação. Utilizam-se dados qualitativos coletados para uma pesquisa mais abrangente, resenhas jornalísticas e entrevistas com lideranças internacionais. Conclui-se com uma reflexão sobre a importância da governança internacional em ensaios clínicos e dos caminhos a serem explorados, visando uma harmonização da diversidade de práticas normativas.
Regenerative medicine involves a paradigm change due to organism regeneration at cellular and tissue level – a controversial contemporary issue and difficult to regulate. This article presents a summary of the main scientific, economic, social and regulatory global trends, analyzed according to relevant theoretical dilemmas in medical anthropology and in the sociology of science and health. This is especially true of the construction of a ‘collective frame of reference’ on the new biological and ontological entities, the shaping of biological citizenship, and governance through uncertainty. Empirical evidence is also presented on a key aspect in regulation and governance, namely the emergence of a new transnational demand in health research through the establishment of parallel markets for ova and experimental cellular therapies. Qualitative data collected for a broader research paper is analyzed, as well as journal reviews and information gathered during interviews with international leaders. The paper concludes with a discussion on the importance on international governance of clinical trials and on further exploration, towards a multilevel harmonization of a diversity of normative practices.
Asunto(s)
Humanos , Animales , Masculino , Femenino , Adulto , Ratones , Uniones Adherentes/metabolismo , Cadherinas/metabolismo , Células Ciliadas Auditivas/metabolismo , Equilibrio Postural/fisiología , Sáculo y Utrículo/metabolismo , Uniones Adherentes/ultraestructura , Animales Recién Nacidos , Recuento de Células , Células Cultivadas , Células Ciliadas Auditivas/citología , Células Ciliadas Auditivas/ultraestructura , Células Ciliadas Vestibulares/citología , Células Ciliadas Vestibulares/metabolismo , Células Ciliadas Vestibulares/ultraestructura , Ratones Transgénicos , Sáculo y Utrículo/embriología , Sáculo y Utrículo/ultraestructuraRESUMEN
The utricle provides critical information about spatiotemporal properties of head movement. It comprises multiple subdivisions whose functional roles are poorly understood. We previously identified four subdivisions in turtle utricle, based on hair bundle structure and mechanics, otoconial membrane structure and hair bundle coupling, and immunoreactivity to calcium-binding proteins. Here we ask whether these macular subdivisions are innervated by distinctive populations of afferents to help us understand the role each subdivision plays in signaling head movements. We quantified the morphology of 173 afferents and identified six afferent classes, which differ in structure and macular locus. Calyceal and dimorphic afferents innervate one striolar band. Bouton afferents innervate a second striolar band; they have elongated terminals and the thickest processes and axons of all bouton units. Bouton afferents in lateral (LES) and medial (MES) extrastriolae have small-diameter axons but differ in collecting area, bouton number, and hair cell contacts (LES >> MES). A fourth, distinctive population of bouton afferents supplies the juxtastriola. These results, combined with our earlier findings on utricular hair cells and the otoconial membrane, suggest the hypotheses that MES and calyceal afferents encode head movement direction with high spatial resolution and that MES afferents are well suited to signal three-dimensional head orientation and striolar afferents to signal head movement onset.
Asunto(s)
Células Ciliadas Vestibulares/ultraestructura , Terminales Presinápticos/ultraestructura , Vestíbulo del Laberinto/ultraestructura , Animales , Axones/ultraestructura , Tortugas , Vestíbulo del Laberinto/inervaciónRESUMEN
Sensory receptors in the vestibular system (hair cells) encode head movements and drive central motor reflexes that control gaze, body movements, and body orientation. In mammals, type I and II vestibular hair cells are defined by their shape, contacts with vestibular afferent nerves, and membrane conductance. Here we describe unique morphological features of type II vestibular hair cells in mature rodents (mice and gerbils) and bats. These features are cytoplasmic processes that extend laterally from the hair cell base and project under type I hair cells. Closer analysis of adult mouse utricles demonstrated that the basolateral processes of type II hair cells vary in shape, size, and branching, with the longest processes extending three to four hair cell widths. The hair cell basolateral processes synapse upon vestibular afferent nerves and receive inputs from vestibular efferent nerves. Furthermore, some basolateral processes make physical contacts with the processes of other type II hair cells, forming some sort of network among type II hair cells. Basolateral processes are rare in perinatal mice and do not attain their mature form until 3-6 weeks of age. These observations demonstrate that basolateral processes are significant signaling regions of type II vestibular hair cells and suggest that type II hair cells may directly communicate with each other, which has not been described in vertebrates.
Asunto(s)
Vías Auditivas/citología , Células Ciliadas Vestibulares , Vestíbulo del Laberinto/citología , Oxidorreductasas de Alcohol , Animales , Vías Auditivas/metabolismo , Proteínas de Unión al Calcio/metabolismo , Recuento de Células , Quirópteros , Colina O-Acetiltransferasa/metabolismo , Proteínas Co-Represoras , Proteínas de Unión al ADN/metabolismo , Células Ciliadas Vestibulares/clasificación , Células Ciliadas Vestibulares/metabolismo , Células Ciliadas Vestibulares/ultraestructura , Mamíferos/anatomía & histología , Ratones , Microscopía Electrónica de Transmisión , Fibras Nerviosas/metabolismo , Fibras Nerviosas/ultraestructura , Red Nerviosa/metabolismo , Red Nerviosa/ultraestructura , Proteínas del Tejido Nervioso/metabolismo , Fosfoproteínas/metabolismo , Ratas , Factores de Transcripción SOXB1/metabolismo , Especificidad de la EspecieRESUMEN
Ototoxicity is a major cause of the loss of hearing and balance in humans. Ototoxic compounds include pharmaceuticals such as aminoglycoside antibiotics, anti-malarial drugs, loop diuretics and chemotherapeutic platinum agents, and industrial chemicals including several solvents and nitriles. Human and rodent data indicate that the main target of toxicity is hair cells (HCs), which are the mechanosensory cells responsible for sensory transduction in both the auditory and the vestibular system. Nevertheless, the compounds may also affect the auditory and vestibular ganglion neurons. Exposure to ototoxic compounds has been found to cause HC apoptosis, HC necrosis, and damage to the afferent terminals, of differing severity depending on the ototoxicity model. One major pathway frequently involved in HC apoptosis is the c-jun N-terminal kinase (JNK) signaling pathway activated by reactive oxygen species, but other apoptotic pathways can also play a role in ototoxicity. Moreover, little is known about the effects of chronic low-dose exposure. In rodent vestibular epithelia, extrusion of live HCs from the sensory epithelium may be the predominant form of cell demise during chronic ototoxicity. In addition, greater involvement of the afferent terminals may occur, particularly the calyx units contacting type I vestibular HCs. As glutamate is the neurotransmitter in this synapse, excitotoxic phenomena may participate in afferent and ganglion neuron damage. Better knowledge of the events that take place in chronic ototoxicity is of great interest, as it will increase understanding of the sensory loss associated with chronic exposure and aging.
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Antiinfecciosos/efectos adversos , Células Ciliadas Auditivas/patología , Células Ciliadas Vestibulares/patología , Enfermedades Neurodegenerativas , Enfermedades Vestibulares , Animales , Células Ciliadas Auditivas/efectos de los fármacos , Células Ciliadas Auditivas/ultraestructura , Células Ciliadas Vestibulares/efectos de los fármacos , Células Ciliadas Vestibulares/ultraestructura , Humanos , Enfermedades Neurodegenerativas/inducido químicamente , Enfermedades Neurodegenerativas/complicaciones , Enfermedades Neurodegenerativas/patología , Enfermedades Vestibulares/inducido químicamente , Enfermedades Vestibulares/complicaciones , Enfermedades Vestibulares/patologíaRESUMEN
Several nitriles, including allylnitrile and cis-crotononitrile, have been shown to be ototoxic and cause hair cell degeneration in the auditory and vestibular sensory epithelia of mice. However, these nitriles can also be lethal due in large part to the microsomal metabolic release of cyanide, which is mostly dependent on the activity of the 2E1 isoform of the cytochrome P450 (CYP2E1). In this study, we co-administered mice with a nitrile and, to reduce their lethal effects, a selective CYP2E1 inhibitor: diallylsulfide (DAS) or trans-1,2-dichloroethylene (TDCE). Both in female 129S1/SvImJ (129S1) mice co-treated with DAS and cis-crotononitrile and in male RjOrl:Swiss/CD-1 (Swiss) mice co-treated with TDCE and allylnitrile, the nitrile caused a dose-dependent loss of vestibular function, as assessed by a specific behavioral test battery, and of hair cells, as assessed by hair bundle counts using scanning electron microscopy. In the experiments, the CYP2E1 inhibitors provided significant protection against the lethal effects of the nitriles and did not diminish the vestibular toxicity as assessed by behavioral effects in comparison to animals receiving no inhibitor. Additional experiments using a single dose of allylnitrile demonstrated that TDCE does not cause hair cell loss on its own and does not modify the vestibular toxicity of the nitrile in either male or female 129S1 mice. In all the experiments, high vestibular dysfunction scores in the behavioral test battery predicted extensive to complete loss of hair cells in the utricles. This provides a means of selecting animals for subsequent studies of vestibular hair cell regeneration or replacement.
Asunto(s)
Compuestos Alílicos/toxicidad , Inhibidores del Citocromo P-450 CYP2E1 , Sordera/patología , Células Ciliadas Vestibulares/patología , Nitrilos/toxicidad , Sulfuros/toxicidad , Animales , Conducta Animal/efectos de los fármacos , Muerte Celular/efectos de los fármacos , Sordera/inducido químicamente , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Femenino , Células Ciliadas Vestibulares/efectos de los fármacos , Células Ciliadas Vestibulares/ultraestructura , Masculino , Ratones , Ratones de la Cepa 129 , Microscopía Electrónica de Rastreo , Pruebas de Función Vestibular , Vestíbulo del Laberinto/efectos de los fármacos , Vestíbulo del Laberinto/patología , Vestíbulo del Laberinto/ultraestructuraRESUMEN
Hair cells reside in specialized epithelia in the inner ear of vertebrates, mediating the detection of sound, motion, and gravity. The transduction of these stimuli into a neuronal impulse requires the deflection of stereocilia, which are stabilized by the actin-rich cuticular plate. Recent electrophysiological studies have implicated the vestibular system in pigeon magnetosensation. Here we report the discovery of a single iron-rich organelle that resides in the cuticular plate of cochlear and vestibular hair cells in the pigeon. Transmission electron microscopy, coupled with elemental analysis, has shown that this structure is composed of ferritin-like granules, is approximately 300-600 nm in diameter, is spherical, and in some instances is membrane-bound and/or organized in a paracrystalline array. This organelle is found in hair cells in a wide variety of avian species, but not in rodents or in humans. This structure may function as (1) a store of excess iron, (2) a stabilizer of stereocilia, or (3) a mediator of magnetic detection. Given the specific subcellular location, elemental composition, and evolutionary conservation, we propose that this structure is an integral component of the sensory apparatus in birds.
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Células Ciliadas Auditivas/metabolismo , Células Ciliadas Vestibulares/metabolismo , Hierro/metabolismo , Orgánulos/metabolismo , Animales , Columbidae , Células Ciliadas Auditivas/ultraestructura , Células Ciliadas Vestibulares/ultraestructura , Microscopía Electrónica de TransmisiónRESUMEN
Inner ear structures are compared among three major genera of the deep-sea fish family Melamphaidae (bigscales and ridgeheads). Substantial interspecific variation is found in the saccular otoliths, including the presence of a unique otolithic "spur" in the genera Melamphaes and Poromitra. The variation in the saccular otolith is correlated with an increase in the number of hair bundle orientation groups on the sensory epithelia from the genera Scopelogadus to Poromitra to Melamphaes. The diverse structural variations found in the saccule may reflect the evolutionary history of these species. The sensory hair cell bundles in this family have the most variable shapes yet encountered in fish ears. In the saccule, most of the hair bundles are 15-20 µm high, an exceptional height for fish otolithic end organs. These bundles have large numbers of stereovilli, including some that reach the length of the kinocilium. In the utricle, the striolar region separates into two unusually shaped areas that have not been described in any other vertebrates. The brains in all species have a relatively small olfactory bulb and optic tectum, as well as an enlarged posterior cerebellar region that is likely to be involved in inner ear and lateral line (octavolateral) functions. Data from melamphaids support the hypothesis that specialized anatomical structures are found in the ears of some (if not most) deep-sea fishes, presumably enhancing their hearing sensitivity.