RESUMEN
Breast-milk αS1-casein is a Toll-like receptor 4 (TLR4) agonist, whereas phosphorylated αS1-casein does not bind TLR4. The objective of this study was to analyse the structural requirements for these effects. In silico analysis of αS1-casein indicated high α-helical content with coiled-coil characteristics. This was confirmed by CD-spectroscopy, showing the α-helical conformation to be stable between pH 2 and 7.4. After in vitro phosphorylation, the α-helical content was significantly reduced, similar to what it was after incubation at 80 °C. This conformation showed no in vitro induction of IL-8 secretion via TLR4. A synthetic peptide corresponding to V77-E92 of αS1-casein induced an IL-8 secretion of 0.95 ng/mL via TLR4. Our results indicate that αS1-casein appears in two distinct conformations, an α-helical TLR4-agonistic and a less α-helical TLR4 non-agonistic conformation induced by phosphorylation. This is to indicate that the immunomodulatory role of αS1-casein, as described before, could be regulated by conformational changes induced by phosphorylation.
Asunto(s)
Caseínas , Leche Humana , Humanos , Caseínas/química , Caseínas/clasificación , Interleucina-8 , Dominios Proteicos , Receptor Toll-Like 4/análisis , Filogenia , Estructura Secundaria de Proteína , Células HEK293RESUMEN
The main challenges in the purification of αS2-casein are due to the low quantity in milk and high homology with other casein subunits, i.e., αS1-casein, ß-casein, and κ-casein. To overcome these challenges, the aim of this study was to develop a two-step purification to isolate native αS2-casein in goat milk from five different breeds; British Alpine, Jamnapari, Saanen, Shami, and Toggenburg. The first step of the purification was executed by anion-exchange chromatography under optimal elution conditions followed by size exclusion chromatography. Tryptic peptides from in-gel digestion of purified αS2-casein were sequenced and analyzed by LC-ESI-MS/MS. From 1.05 g of whole casein, the highest yield of αS2-casein (6.7 mg/mL) was obtained from Jamnapari and the lowest yield (2.2 mg/mL) was from Saanen. A single band of pure αS2-casein was observed on SDS-PAGE for all breeds. The αS2-casein showed coverage percentage of amino acid sequence from 76.68 to 92.83%. The two-step purification process developed herein was successfully applied for isolating native αS2-casein from goat milk with high purity, which will allow for future in vitro studies to be conducted on this protein.
Asunto(s)
Caseínas , Cromatografía Liquida/métodos , Cabras/clasificación , Leche/química , Animales , Caseínas/análisis , Caseínas/química , Caseínas/clasificación , Caseínas/aislamiento & purificación , Cabras/metabolismo , Leche/clasificación , Espectrometría de Masa por Ionización de Electrospray/métodos , Espectrometría de Masas en Tándem/métodosRESUMEN
Ageing is often characterised by nutritional deficiencies and functional alterations of the digestive and immune system. The aim of the present study was to analyse the impact of consumption of conventional milk with A1/A2 beta-casein, compared to milk containing only the A2 beta-casein variant, characterised by a protein profile favouring gut health. Twenty-four ageing Balb-c mice (20 months old) were fed for 4 weeks, with either a control diet (CTRL), a diet supplemented with bovine milk containing A1/A2 beta-casein (A1A2) or a diet containing A2/A2 beta-casein (A2A2). Lymphocyte subpopulations, enzymatic activities, cytokine secretion, gut morphology and histopathological alterations were measured in different gut segments, while short-chain fatty acids (SCFAs) content and microbiota composition were evaluated in faecal samples. The A2A2 group showed higher content of faecal SCFAs (in particular, isobutyrate) of intestinal CD4+ and CD19+ lymphocytes in the intraepithelial compartment and improved villi tropism. The A1A2 group showed higher percentages of intestinal TCRγδ+ lymphocytes. Faecal microbiota identified Deferribacteriaceae and Desulfovibrionaceae as the most discriminant families for the A2A2 group, while Ruminococcaceae were associated to the A1A2 group. Taken together, these results suggest a positive role of milk, in particular when containing exclusively A2 beta-casein, on gut immunology and morphology of an ageing mice model.
Asunto(s)
Caseínas/administración & dosificación , Caseínas/farmacología , Suplementos Dietéticos , Microbioma Gastrointestinal , Mucosa Intestinal/inmunología , Mucosa Intestinal/microbiología , Leche , Fenómenos Fisiológicos de la Nutrición/fisiología , Animales , Caseínas/clasificación , Citocinas/metabolismo , Ácidos Grasos Volátiles/metabolismo , Femenino , Inflamación , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Linfocitos/inmunología , Masculino , Ratones Endogámicos BALB C , Leche/química , Modelos AnimalesRESUMEN
Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, ß, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits. Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values. A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs. Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count). Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively). Components standard prediction errors decreased, and accuracies and reliabilities increased when genetic effects were considered. Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
Asunto(s)
Caseínas/genética , Epistasis Genética , Cabras/genética , Leche/química , Animales , Cruzamiento , Caseínas/química , Caseínas/clasificación , Genoma , Genómica , Lactancia/genética , Lactosa/genética , Proteínas de la Leche , Polimorfismo de Nucleótido Simple/genéticaRESUMEN
Caseins are a family of proteins constituted by α-caseins (αs-1 and αs-2 caseins), ß-caseins and κ-caseins. ß-caseins, in particular, show a temperature and concentration-dependent self-assembling behaviour. Recently, ß-casein micelles have been proposed as natural nanocarriers for the delivery of hydrophobic compounds, promoting their bioavailability. Until now, all studies regarding both chemical-physical characterization and applications of ß-caseins have employed the protein of bovine origin. However, it could be interesting to exploit the use of ß-caseins from other milk sources for their potential encapsulation ability and immunogenicity but, at present, no information on the self-assembling behaviour is available for ß-caseins from the milk of species different from bovine. In this work, for the first time, ß-caseins from human milk and from donkey, goat, and sheep milk were purified and their self-assembling behaviour was compared to that of a commercial bovine ß-casein, the only one for which the concentration and temperature aggregation behaviour is known. Furthermore, a preliminary evaluation of the immunogenicity potential of ß-casein from other milk sources has been performed by cross-reaction experiments using anti-ß-casein antibodies from bovine origin. The results indicated a similar self-assembling profile among all ß-caseins examined compared to the bovine ß-casein, suggesting the possible use of ß-casein from other milk sources as nanocarriers. Since donkey and human ß-casein do not cross-react with bovine anti-ß-casein antibodies, they could be particularly interesting for the development of self-assembling systems with lower hypoallergenic potential.
Asunto(s)
Anticuerpos/metabolismo , Caseínas/metabolismo , Leche/química , Animales , Anticuerpos/aislamiento & purificación , Especificidad de Anticuerpos , Caseínas/química , Caseínas/clasificación , Caseínas/aislamiento & purificación , Bovinos , Reacciones Cruzadas , Equidae , Femenino , Colorantes Fluorescentes/química , Cabras , Humanos , Micelas , Pirenos/química , Ovinos , Especificidad de la Especie , Espectrometría de Fluorescencia , TemperaturaRESUMEN
The present work examined the role of Tyr and Trp in oxidative modifications of caseins, the most abundant milk proteins, induced by peroxyl radicals (ROOâ¢). We hypothesized that the selectivity of ROO⢠and the high flexibility of caseins (implying a high exposure of Tyr and Trp residues) would favor radical-radical reactions, and di-tyrosine (di-Tyr) and di-tryptophan (di-Trp) formation. Solutions of α- and ß-caseins were exposed to ROO⢠from thermolysis and photolysis of AAPH (2,2'-azobis(2-methylpropionamidine)dihydrochloride). Oxidative modifications were examined using electrophoresis, western blotting, fluorescence, and chromatographic methodologies with diode array, fluorescence and mass detection. Exposure of caseins to AAPH at 37⯰C gave fragmentation, cross-linking and protein aggregation. Amino acid analysis showed consumption of Trp, Tyr, Met, His and Lys residues. Quantification of Trp and Tyr products, showed low levels of di-Tyr and di-Trp, together with an accumulation of carbonyls indicating that casein aggregation is, at least partly, associated with secondary reactions between carbonyls and Lys and His residues. AAPH photolysis, which generates a high flux of free radicals increased the extent of formation of di-Tyr in both model peptides and α- and ß- caseins; di-Trp was only detected in peptides and α-casein. Thus, in spite of the high flexibility of caseins, which would be expected to favor radical-radical reactions, the low flux of ROO⢠generated during AAPH thermolysis disfavours the formation of dimeric radical-radical cross-links such as di-Tyr and di-Trp, instead favoring other O2-dependent crosslinking pathways such as those involving secondary reactions of initial carbonyl products.
Asunto(s)
Amidinas/química , Caseínas/química , Fragmentos de Péptidos/química , Peróxidos/farmacología , Agregado de Proteínas/efectos de los fármacos , Triptófano/química , Tirosina/química , Animales , Caseínas/clasificación , Bovinos , Cinética , Oxidantes/química , Oxidación-Reducción , Peróxidos/químicaRESUMEN
The PRM/Alf inbred mice exhibit a huge intestinal lengthening. Since milk contains bioactive factors implied in numerous biological processes, one hypothesis is that PRM/Alf milk contains intestinotrophic factors contributing to this remarkable phenotype. A comparison between the milk from PRM/Alf and C57BL/6J (as a control) strains could be helpful in the identification of such factors, including proteins. However, a complete description of the mouse milk major protein fraction is still missing. Hence we adapted a reliable technique to separate and identify the major mouse milk proteins. This approach was achieved through the protein study of milk from C57BL/6J and PWK/Pas strains representative of two Mus musculus subspecies, M. m. domesticus and M. m. musculus respectively. C57BL/6J milk samples were first skimmed and fractionated by reverse phase-HPLC (RP-HPLC). The protein content of each chromatographic peak was analysed by SDS-PAGE and identified by mass spectrometry. This methodological approach allowed characterization of nine major mouse milk proteins: alpha(s1), beta, gamma, epsilon and kappa-caseins, Whey Acidic Protein, lactoferrin, Serum Albumin, Fatty Acid Binding Protein, as well as an alpha(s1)-casein isoform. Then, RP-HPLC patterns of C57BL/6J milk proteins were compared with those obtained starting from the milk of PWK/Pas females. This comparison revealed a protein polymorphism for the alpha(s1)-casein.
Asunto(s)
Caseínas/análisis , Lactancia/fisiología , Proteínas de la Leche/análisis , Leche/química , Proteómica/métodos , Animales , Caseínas/clasificación , Femenino , Ratones , Ratones Endogámicos , Isoformas de Proteínas/análisis , Valores de Referencia , Especificidad de la EspecieRESUMEN
We have recently shown that sodium caseinate (CasNa) was able to inhibit the proliferation of the myeloid cell line 32D cl3 in a non-toxic way, and that it also induced the expression of macrophage colony-stimulating factor (M-CSF). Casein is the main protein present in milk and is composed of alpha (alpha), beta (beta) and kappa (kappa) subunits. This work was undertaken to evaluate if any one casein is responsible for the proliferation and differentiation properties found for CasNa on myeloid cells. Taking into consideration that 32D cl3 cells are considered to be non-malignant and dependent on IL-3 for proliferation, we also included for this study a leukemic cell line, WEHI-3, that does not depend on any external growth factor for its proliferation in order to evaluate if the growth inhibitory effect of caseins is also present for malignant cells. Our results showed that all caseins were inhibitory for the proliferation of either 32D cl3 and WEHI-3 and that only the 32D cl3 cells were induced to differentiate into the monocyte-macrophage lineage. In order to evaluate if CasNa was able to inhibit the proliferation of other myeloid cells we used J774 and P388 and found that they were also inhibited. We also determined that the different caseins exhibit different differentiation properties, with alpha-casein being the only one able to induce the secretion of M-CSF. We consider this work to open a new field of research, where casein, or its components, can be studied for their possible role in hematopoiesis and on the inhibition of malignant cell proliferation for therapeutic use.
Asunto(s)
Caseínas/farmacología , Diferenciación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Mieloides/efectos de los fármacos , Animales , Caseínas/clasificación , Línea Celular Tumoral , Células Cultivadas , Ratones , Células Mieloides/patología , ARN Mensajero/biosíntesis , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
This report of the American Dairy Science Association Committee on the Nomenclature, Classification, and Methodology of Milk Proteins reviews changes in the nomenclature of milk proteins necessitated by recent advances of our knowledge of milk proteins. Identification of major caseins and whey proteins continues to be based upon their primary structures. Nomenclature of the immunoglobulins consistent with new international standards has been developed, and all bovine immunoglobulins have been characterized at the molecular level. Other significant findings related to nomenclature and protein methodology are elucidation of several new genetic variants of the major milk proteins, establishment by sequencing techniques and sequence alignment of the bovine caseins and whey proteins as the reference point for the nomenclature of all homologous milk proteins, completion of crystallographic studies for major whey proteins, and advances in the study of lactoferrin, allowing it to be added to the list of fully characterized milk proteins.
Asunto(s)
Proteínas de la Leche/química , Proteínas de la Leche/clasificación , Terminología como Asunto , Secuencia de Aminoácidos , Animales , Caseínas/química , Caseínas/clasificación , Bovinos , Inmunoglobulinas/química , Inmunoglobulinas/clasificación , Lactoglobulinas , Datos de Secuencia Molecular , Peso Molecular , Análisis de Secuencia de Proteína , Homología de Secuencia de Aminoácido , Proteína de Suero de LecheRESUMEN
A short stretch of 13 amino acids in the central portion of human beta-casein contains four positively charged conserved residues, three Lys and one Arg. We changed these individually to Glu, reversing their charge, and compared the resulting recombinant proteins to the wild-type recombinant, monitoring thermal aggregation with turbidity as well as using the fluorescence of the intrinsic Trp, of hydrophobically bound ANS and fluorescence resonance energy transfer from Trp to ANS to detect differences in structure. The results demonstrate the need to maintain the actual or functional identity of these conserved charged amino acid residues in order to attain the protein folding and functional properties of the wild-type human beta-casein molecule. They emphasize the probability that native human beta-casein has a unique folding pattern that is important for its function of suspending minerals and delivering the protein and minerals to the neonate in a readily ingestible form.
Asunto(s)
Aminoácidos/química , Caseínas/química , Caseínas/clasificación , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Naftalenosulfonatos de Anilina , Secuencia Conservada , Transferencia Resonante de Energía de Fluorescencia , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Mutación , Nefelometría y Turbidimetría , Fosforilación , Conformación Proteica , Desnaturalización Proteica , Proteínas Recombinantes/química , Electricidad Estática , Relación Estructura-Actividad , Temperatura , Triptófano/químicaAsunto(s)
Caseínas/administración & dosificación , Caseínas/metabolismo , Quelantes/administración & dosificación , Quelantes/metabolismo , Dieta Aterogénica , Leche/química , Leche/metabolismo , Animales , Enfermedades de las Arterias Carótidas/metabolismo , Arteria Carótida Interna/metabolismo , Arteria Carótida Interna/patología , Caseínas/clasificación , Quelantes/clasificación , Colesterol en la Dieta/administración & dosificación , HDL-Colesterol/sangre , LDL-Colesterol/sangre , HumanosRESUMEN
Recombinant wild-type non-phosphorylated human beta-casein was obtained from Escherichia coli. Turbidity vs. temperature (T) without Ca(2+) showed wild-type self-association like native except for irreversibility upon T-cycling with the original pattern re-established after concentrated urea/dialysis. With Ca(2+), wild-type was more native-like. Intrinsic Trp fluorescence spectra were similar but with lowered intensity for the wild-type protein. Changes in extrinsic ANS fluorescence from 4 to 37 degrees C showed less exposure of hydrophobic surface for wild-type than native. Trp to ANS fluorescence resonance energy transfer was higher for wild-type than native at 4 degrees C but 2- to 3-fold lower at 37 degrees C. The native protein must be directed by the environment and/or a chaperone to fold into a unique, somewhat flexible, conformation, unaltered by urea during purification. Wild-type protein, with many native properties, does not spontaneously fold to the native conformation, even after solubilization with urea. T-cycling gives a stable conformation that is different from the native.
Asunto(s)
Caseínas/química , Caseínas/clasificación , Escherichia coli/metabolismo , Transferencia Resonante de Energía de Fluorescencia/métodos , Caseínas/biosíntesis , Caseínas/genética , Clonación Molecular , Escherichia coli/química , Escherichia coli/genética , Humanos , Leche Humana/química , Leche Humana/metabolismo , Mutagénesis Sitio-Dirigida , Nefelometría y Turbidimetría , Fosforilación , Conformación Proteica , Pliegue de Proteína , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genéticaRESUMEN
Kappa-casein is a mammalian milk protein involved in a number of important physiological processes. In the gut, the ingested protein is split into an insoluble peptide (para kappa-casein) and a soluble hydrophilic glycopeptide (caseinomacropeptide). Caseinomacropeptide is responsible for increased efficiency of digestion, prevention of neonate hypersensitivity to ingested proteins, and inhibition of gastric pathogens. Variation within this peptide has significant effects associated with important traits such as milk production. The nucleotide sequences for regions of kappa-casein exon and intron four were determined for representatives of the artiodactyl family Bovidae. The pattern of nucleotide substitution in kappa-casein sequences for distantly related bovid taxa demonstrates that positive selection has accelerated their divergence at the amino acid sequence level. This selection has differentially influenced the molecular evolution of the two kappa-casein split peptides and is focused within a 34-codon region of caseinomacropeptide.
Asunto(s)
Caseínas/genética , Evolución Molecular , Rumiantes/genética , Selección Genética , Animales , Bison/genética , Caseínas/clasificación , Bovinos , Exones , IntronesRESUMEN
To assess phylogenetic relationships among the higher ruminants (infraorder Pecora, order Artiodactyla), we analyzed K-casein DNA sequences, including 434 nucleotides of the fourth exon. The higher ruminant families Bovidae, Cervidae, Giraffidae, and Antilocapridae each have monophyletic K-casein sequences. Maximum parsimony and distance analyses identify Giraffidae as a sister group to either Cervidae or a Bovidae-Cervidae clade and Antilocapridae as a sister group to a Bovidae-Cervidae-Giraffidae clade. At a higher level these four families occur as a monophyletic clade relative to Tragulidae and Suidae. Within Cervidae, the subfamily Odocoileinae is monophyletic and Cervinae and Muntiacinae occur as independent lineages within a separate clade. Within Bovidae, the subfamilies Bovinae and Caprinae are monophyletic. Genera within Cervinae (Cervus, Elaphurus) and Bovinae (Bison, Bos) are paraphyletic. There is intraspecific allelic variation in Cervus elaphus, Odocoileus hemionus, and Bison bison. The rate of K-casein fourth exon DNA sequence evolution is estimated to be about 0.004 nucleotide substitutions per million years. The K-casein phylogeny is discussed relative to other molecular and morphological data.
Asunto(s)
Artiodáctilos/clasificación , Caseínas/genética , Filogenia , Animales , Caseínas/clasificación , ADN , Exones , Datos de Secuencia Molecular , Rumiantes/clasificación , Rumiantes/genética , Alineación de SecuenciaRESUMEN
Casein in human milk is believed to serve several biological functions in newborns. However, the content and subunit composition of human casein has so far received little attention. We recently developed a method to separate human-milk whey and casein by adjustment of whole human milk to pH 4.3 and addition of calcium followed by ultracentrifugation. In this study we analyzed and evaluated human casein prepared by different methods. We used fast protein liquid chromatography (FPLC) with an anion-exchange column (Mono-Q) and polyacrylamide gradient gel electrophoresis techniques to analyze the casein subunit composition. Total casein in human milk, as determined by the Kjeldahl method, varies during lactation; the casein content is approximately 20% of the total protein content in early lactation and 45% in late lactation. We found differences in both glycosylation and phosphorylation patterns of kappa-caseins and beta-caseins from premature and term milk samples.