RESUMEN
OBJECTIVES: To investigate the antihypertensive effect of crude extract of Chenopodium album (Ca.Cr), based on its medicinal use in hypertension. METHODS: Ca.Cr and its fractions were tested in-vivo in normotensive anesthetized rats for blood pressure-lowering effect. In-vitro experiments were performed on isolated rat aortae to explore the vascular mechanism(s). RESULTS: In normotensive anesthetized rats, Ca.Cr produced a dose-dependent (1-300mg/kg) fall (30%mmHg) in mean arterial pressure (MAP). Among the fractions, nHexane was the most potent (46% fall). In rat aortic rings precontracted with phenylephrine (PE), Ca.Cr and its fractions (except Ca.Aq) produced endothelium-dependent vasorelaxation, which was partially reversed with endothelium removal and by pretreating intact aortic rings with L-NAME (10µM) and atropine (1µM). This relaxation to Ca.Cr and fractions (nHexane, ethylacetate and chloroform) was also eliminated with indomethacin pretreatment, however, it unmasked a vasoconstriction effect with Ca.Cr only. Surprisingly, the aqueous fraction produced a calcium sensitive strong vasoconstriction instead of vasorelaxation. The crude extract and its fractions (except Ca.Aq) also antagonized vasoconstriction induced with high K+ (80mM), suggesting calcium antagonistic effect. The aqueous fraction produced mild vasorelaxation against high K+. This effect was further confirmed when pretreatment of the aortic rings with different concentrations of crude extract and fractions suppressed CaCl2 concentration response curves, similar to verapamil. In acute toxicity test, Ca.Cr extract was found safe up to 5g/kg body weight in mice. CONCLUSION: These findings suggest that crude extract and fractions of C. album produced vasorelaxant effect through muscarinic receptors linked-NO pathway, prostaglandin (endothelium-dependent) and calcium antagonism (endothelium-independent), which explains the blood pressure lowering effect of C. album in rats.
Asunto(s)
Chenopodium album , Vasodilatación , Ratas , Animales , Ratones , Presión Sanguínea , Chenopodium album/metabolismo , Calcio/metabolismo , Calcio/farmacología , Extractos Vegetales/farmacología , Ratas Sprague-Dawley , Vasodilatadores/farmacología , Bloqueadores de los Canales de Calcio , Endotelio/metabolismo , Endotelio Vascular/metabolismoRESUMEN
Common lambsquarters (Chenopodium album L.) is a broadleaf weed that can severely damage soybean fields. Two C. album populations (1744 and 1731) suspected resistant to imazethapyr were investigated for resistance levels to imazethapyr, thifensulfuron-methyl, and fomesafen and their resistance mechanisms were investigated. Whole-plant dose-response assays revealed that, compared to the susceptible (S) population, the 1744 population was 16.5-fold resistant to imazethapyr, slightly resistant to thifensulfuron-methyl (resistance index [R/S], <3). The 1731 population was 18.8-fold resistant to imazethapyr, 2.9-fold resistant to thifensulfuron-methyl, and 5.1-fold resistant to fomesafen. In vitro acetolactate synthase (ALS) assays showed 17.1-fold and 19.3-fold resistance levels of 1744 and 1731 populations to imazethapyr respectively. ALS gene sequence analysis identified Ala122Thr amino acid substitution in the 1744 population and Ser653Thr amino acid substitution in the 1731 population. No mutations of the protoporphyrinogen oxidase (PPO) gene were detected. However, pre-treatment with malathion reversed fomesafen resistance, suggesting nontarget-site resistance mechanisms likely play a role in the 1731 population.
Asunto(s)
Acetolactato Sintasa , Chenopodium album , Herbicidas , Acetolactato Sintasa/genética , Acetolactato Sintasa/metabolismo , Chenopodium album/genética , Chenopodium album/metabolismo , Resistencia a los Herbicidas/genética , Herbicidas/farmacología , Protoporfirinógeno-OxidasaRESUMEN
Proto-Kranz plants represent an initial phase in the evolution from C3 to C3-C4 intermediate to C4 plants. The ecological and adaptive aspects of C3-C4 plants would provide an important clue to understand the evolution of C3-C4 plants. We investigated whether growth temperature and nitrogen (N) nutrition influence the expression of C3-C4 traits in Chenopodium album (proto-Kranz) in comparison with Chenopodium quinoa (C3). Plants were grown during 5 weeks at 20 or 30 °C under standard or low N supply levels (referred to as 20SN, 20LN, 30SN, and 30LN). Net photosynthetic rate and leaf N content were higher in 20SN and 30SN plants than in 20LN and 30LN plants of C. album but did not differ among growth conditions in C. quinoa. The CO2 compensation point (Γ) of C. album was lowest in 30LN plants (36 µmol mol-1), highest in 20SN plants (51 µmol mol-1), and intermediate in 20LN and 30SN plants, whereas Γ of C. quinoa did not differ among the growth conditions (51-52 µmol mol-1). The anatomical structure of leaves was not considerably affected by growth conditions in either species. However, ultrastructural observations in C. album showed that the number of mitochondria per mesophyll or bundle sheath (BS) cell was lower in 20LN and 30LN plants than in 20SN and 30SN plants. Immunohistochemical observations revealed that lower accumulation level of P-protein of glycine decarboxylase (GDC-P) in mesophyll mitochondria than in BS mitochondria is the major factor causing the decrease in Γ values in C. album plants grown under low N supply and high temperature. These results suggest that high growth temperature and low N supply lead to the expression of C3-C4 traits (the reduction of Γ) in the proto-Kranz plants of C. album through the regulation of GDC-P expression.
Asunto(s)
Chenopodium album , Chenopodium album/metabolismo , Glicina-Deshidrogenasa (Descarboxilante)/metabolismo , Nitrógeno , Fotosíntesis , Hojas de la Planta/metabolismo , TemperaturaRESUMEN
The application of minimal doses of herbicides is very popular due to concerns about the negative impacts of herbicides on the environment and public health. Studies were conducted to estimate the possibility of using quick and non- destructive methods to investigate Chenopodium album L. and Abutilon theophrasti Medik. response to mesotrione. The studies were conducted in a controlled environment to determine the response of C. album and A. theophrasti to mesotrione using dose-response curves created based on plant dry weight, chlorophyll fluorescence parameters and chlorophyll content. The obtained effective dose values showed that the studied weeds were susceptible to reduced doses of mesotrione. ED95 values estimated for both species for dry weight and chlorophyll fluorescence parameters were lower than the recommended dose rate (120 g a.i. ha-1), with less than 85 g a.i. ha-1 needed to achieve a reduction of 95%, compared with untreated plants, while ED95 value (A. theophrasti: 182 g a.i. ha-1 and C. album: 180 g a.i. ha-1) for chlorophyll content for both species was above the recommended dose rates. Consequently, dry weight and the chlorophyll fluorescence parameters are suitable for estimating the plant response to mesotrione, while chlorophyll content is not.
Asunto(s)
Chenopodium album/efectos de los fármacos , Ciclohexanonas/farmacología , Herbicidas/farmacología , Malvaceae/efectos de los fármacos , Chenopodium album/metabolismo , Clorofila/metabolismo , Ciclohexanonas/administración & dosificación , Relación Dosis-Respuesta a Droga , Herbicidas/administración & dosificación , Malvaceae/metabolismo , Malezas/efectos de los fármacosRESUMEN
MAIN CONCLUSION: Detrimental pleiotropic effects of resistance mutation(s) were observed for multiple-resistant phenotypes (resistant to both atrazine and dicamba). The multiple-resistant phenotypes had lower growth rates and less capacity for vegetative growth compared to the phenotypes only resistant to atrazine. The fitness costs that are conferred by herbicide resistance alleles can affect the rate of herbicide resistance evolution within populations. We evaluated the direct fitness costs involved with multiple resistance to dicamba and atrazine (R1 and R2) in Chenopodium album by comparing the performance of multiple-resistant phenotypes to those phenotypes that were only resistant to atrazine (S1 and S2). The R1 and R2 phenotypes were consistently shorter and produced less dry matter than the S1 and S2 phenotypes. The R1 and R2 phenotypes were shown to have lower relative growth rates (RGR) and net assimilation rates (NAR) than the S1 and S2 phenotypes at an early stage of growth. However, there was no significant difference in RGR between the R1 and R2 and, S1 and S2 phenotypes at a later stage of growth, though the R1 and R2 phenotypes still had a lower NAR at this later stage. Further investigations using a neighbouring crop competition approach showed that the R1 and R2 phenotypes were weaker competitors, and exhibited significantly less capacity for vegetative growth compared to the S1 and S2 phenotypes during competition. Overall, the results of this study revealed multiple- resistance to atrazine and dicamba endowed a significant fitness penalty to C. album, and it is possible that the frequency of multiple-resistant individuals would gradually decline once selection pressure from herbicides was discontinued.
Asunto(s)
Atrazina/farmacología , Chenopodium album/efectos de los fármacos , Dicamba/farmacología , Resistencia a los Herbicidas , Herbicidas/farmacología , Biomasa , Chenopodium album/crecimiento & desarrollo , Chenopodium album/metabolismo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismoRESUMEN
We investigated the effects of near-infrared irradiation on the photoconversion of Chenopodium album water-soluble chlorophyll-binding protein (CaWSCP) in the presence of sodium hydrosulfite and found a further photoconversion from CP742 to CP763, a novel form of CaWSCP. Interestingly, one-third of the absorption peak at 668 nm was recovered in CP763, but re-irradiation under oxidative conditions eliminated the photo convertibility of CaWSCP.
Asunto(s)
Chenopodium album/metabolismo , Chenopodium album/efectos de la radiación , Proteínas de Unión a Clorofila/química , Proteínas de Unión a Clorofila/metabolismo , Rayos Infrarrojos , Agua/química , SolubilidadRESUMEN
Non-photosynthetic and hydrophilic chlorophyll (Chl) proteins, called water-soluble Chl-binding proteins (WSCPs), are distributed in various species of Chenopodiaceae, Amaranthaceae, Polygonaceae and Brassicaceae. Based on their photoconvertibility, WSCPs are categorised into two classes: Class I (photoconvertible) and Class II (non-photoconvertible). Chenopodium album WSCP (CaWSCP; Class I) is able to convert the chlorin skeleton of Chl a into a bacteriochlorin-like skeleton under light in the presence of molecular oxygen. Potassium iodide (KI) is a strong inhibitor of the photoconversion. Because KI attacks tyrosine residues in proteins, tyrosine residues in CaWSCP are considered to be important amino acid residues for the photoconversion. Recently, we identified the gene encoding CaWSCP and found that the mature region of CaWSCP contained four tyrosine residues: Tyr13, Tyr14, Tyr87 and Tyr134. To gain insight into the effect of the tyrosine residues on the photoconversion, we constructed 15 mutant proteins (Y13A, Y14A, Y87A, Y134A, Y13-14A, Y13-87A, Y13-134A, Y14-87A, Y14-134A, Y87-134A, Y13-14-87A, Y13-14-134A, Y13-87-134A, Y14-87-134A and Y13-14-87-134A) using site-directed mutagenesis. Amazingly, all the mutant proteins retained not only chlorophyll-binding activity, but also photoconvertibility. Furthermore, we found that KI strongly inhibited the photoconversion of Y13-14-87-134A. These findings indicated that the four tyrosine residues are not essential for the photoconversion.
Asunto(s)
Chenopodium album/metabolismo , Proteínas de Unión a Clorofila/metabolismo , Clorofila/metabolismo , Fotosíntesis , Tirosina/metabolismo , Secuencia de Aminoácidos , Clorofila A , Luz , Mutación , SolubilidadRESUMEN
BACKGROUND: The objective of the present study was to determine the contribution of the active ingredient (AI) and surfactant, and their concentrations, to the foliar uptake of agrichemicals, and to examine the physical properties that would need to be included in a model for foliar uptake. RESULTS: All spray formulation component variables significantly affected uptake, explaining 73% of the deviance. The deviance explained by each factor ranged from 43% (AI concentration nested within AI) to 5.6% (surfactant). The only significant interaction was between AI and surfactant, explaining 15.8% of the deviance. Overall, 90% of the deviance could be explained by the variables and their first-order interactions. CONCLUSIONS: Uptake increased with increasing lipophilicity of the AI at concentrations below those causing precipitation on the leaf surface. AI concentration had a far greater (negative) effect on the uptake of the lipophilic molecule epoxiconazole. The uptake of 2-deoxy-D-glucose (DOG) and 2,4-dichlorophenoxyacetic acid (2,4-D) increased with increasing hydrophile-lipophile balance (HLB) of the surfactant, the effect of HLB being far greater on the hydrophilic molecule DOG. However, the differences observed in epoxiconazole uptake owing to the surfactant were strongly positively related to the spread area of the formulation on the leaf surface. For all AIs, uptake increased in a similar manner with increasing molar surfactant concentration.
Asunto(s)
Ácido 2,4-Diclorofenoxiacético/metabolismo , Chenopodium album/metabolismo , Desoxiglucosa/metabolismo , Compuestos Epoxi/metabolismo , Hojas de la Planta/metabolismo , Triazoles/metabolismo , Fungicidas Industriales/metabolismo , Herbicidas/metabolismo , Interacciones Hidrofóbicas e Hidrofílicas , TensoactivosRESUMEN
Various plants possess hydrophilic chlorophyll (Chl) proteins known as water-soluble Chl-binding proteins (WSCPs). WSCPs exist in two forms: Class I and Class II, of which Class I alone exhibits unique photoconvertibility. Although numerous genes encoding Class II WSCPs have been identified and the molecular properties of their recombinant proteins have been well characterized, no Class I WSCP gene has been identified to date. In this study, we cloned the cDNA and a gene encoding the Class I WSCP of Chenopodium album (CaWSCP). Sequence analyses revealed that CaWSCP comprises a single exon corresponding to 585bp of an open reading frame encoding 195 amino acid residues. The CaWSCP protein sequence possesses a signature of DUF538, a protein superfamily of unknown function found almost exclusively in Embryophyta. The recombinant CaWSCP was expressed in Escherichia coli as a hexa-histidine fusion protein (CaWSCP-His) that removes Chls from the thylakoid. Under visible light illumination, the reconstituted CaWSCP-His was successfully photoconverted into a different pigment with an absorption spectrum identical to that of native CaWSCP. Interestingly, while CaWSCP-His could bind both Chl a and Chl b, photoconversion occurred only in CaWSCP-His reconstituted with Chl a.
Asunto(s)
Chenopodium album/metabolismo , Proteínas de Unión a Clorofila/genética , Secuencia de Aminoácidos , Secuencia de Bases , Proteínas de Unión a Clorofila/química , Proteínas de Unión a Clorofila/metabolismo , Clonación Molecular , Datos de Secuencia Molecular , Análisis de Secuencia de ProteínaRESUMEN
Allergen specific immunotherapy is the only remedy to prevent the progression of allergic diseases. Nowadays, using of recombinant allergens with reduced IgE-binding capacity is an ideal tool for allergen immunotherapy. Therefore, in this study we focused on a hybrid molecule (HM) production with reduced IgE reactivity from Chenopodium album pollen allergens. By means of genetic engineering, a head to tail structure of the three allergens of the C. album pollen was designed. The resulting DNA construct coding for a 46kDa HM was inserted into an expression vector and expressed as hexahistidine tagged fusion protein in Escherichia coli. IgE reactivity of the HM was evaluated by western blotting, inhibition ELISA and in vivo skin prick test and its immunogenic property was tested by proliferation assay. The recombinant HM was expressed and purified by nickel-affinity chromatography. Comparison of the recombinant HM with a mixture of three recombinant allergens, as well as natural allergens in the whole C. album pollen extract via immunological experiments revealed that it has a much lower potential of IgE reactivity. Furthermore, in vivo skin prick tests showed that it has a significantly lower potency to induce cutaneous reactions than the mixture of recombinant wild type allergens and whole extract while, it had been preserved immunogenic properties. Our results have demonstrated that assembling three allergens of C. album in a hybrid molecule can reduce its IgE reactivity.
Asunto(s)
Alérgenos/metabolismo , Chenopodium album/inmunología , Inmunoglobulina E/metabolismo , Polen/química , Proteínas Recombinantes de Fusión/metabolismo , Alérgenos/química , Alérgenos/genética , Alérgenos/inmunología , Secuencia de Aminoácidos , Animales , Antígenos de Plantas/química , Antígenos de Plantas/genética , Antígenos de Plantas/inmunología , Antígenos de Plantas/metabolismo , Western Blotting , Proteínas de Unión al Calcio/química , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/inmunología , Proteínas de Unión al Calcio/metabolismo , Chenopodium album/química , Chenopodium album/genética , Chenopodium album/metabolismo , Desensibilización Inmunológica/métodos , Ensayo de Inmunoadsorción Enzimática , Femenino , Ingeniería Genética/métodos , Humanos , Hipersensibilidad Inmediata/inmunología , Hipersensibilidad Inmediata/terapia , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Polen/genética , Polen/inmunología , Proteínas Recombinantes de Fusión/química , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/inmunología , Pruebas CutáneasRESUMEN
Lead (Pb) has great toxicity to human beings and other livings. Although there are varied ways to rehabilitate the Pb contaminated area, phytoremediation of Pb pollution in arid lands is still a difficult task, it is therefore urgent to find and identify Pb tolerant plants in arid areas. The physiological responses and tolerance mechanisms to Pb stress (expressed as the Pb concentration, e.g., 0, 50, 150, 300, 600, 800, 1000 mg/L) were investigated for the xerophils Salsola passerina Bunge and Chenopodium album L. Results indicated that S. passerina exhibited higher Pb tolerance than Ch. album in terms of the seed germination rate, bio-activities of SOD and POD, and lower MDA production. There were two ways for S. passerina to reduce Pb toxicity in organism level, e.g., cell wall precipitation and state transfer of free Pb into anchorage. These findings demonstrate that S. passerina is a Pb tolerant species and may have potential application in phytoremediation of Pb contaminated arid lands.
Asunto(s)
Chenopodium album/efectos de los fármacos , Plomo/toxicidad , Salsola/efectos de los fármacos , Contaminantes del Suelo/toxicidad , Biodegradación Ambiental , Chenopodium album/crecimiento & desarrollo , Chenopodium album/metabolismo , Germinación/efectos de los fármacos , Plomo/metabolismo , Malondialdehído/metabolismo , Peroxidasa/metabolismo , Fotosíntesis/efectos de los fármacos , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Proteínas de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Tallos de la Planta/efectos de los fármacos , Tallos de la Planta/crecimiento & desarrollo , Tallos de la Planta/metabolismo , Salsola/crecimiento & desarrollo , Salsola/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Semillas/efectos de los fármacos , Semillas/crecimiento & desarrollo , Contaminantes del Suelo/metabolismo , Superóxido Dismutasa/metabolismoRESUMEN
BACKGROUND: In recent years, common lambsquarters (Chenopodium album L.) populations from sugar beet fields in different European countries have responded as resistant to the as-triazinone metamitron. The populations have been found to have the same D1 point mutation as known for atrazine-resistant biotypes (Ser264 to Gly). However, pot experiments revealed that metamitron resistance is not as clear-cut as observed with triazine resistance in the past. The objectives of this study were to clarify the absorption, translocation and metabolic fate of metamitron in C. album. RESULTS: Root absorption and foliar absorption experiments showed minor differences in absorption, translocation and metabolism of metamitron between the susceptible and resistant C. album populations. A rapid metabolism in the C. album populations was observed when metamitron was absorbed by the roots. The primary products of metamitron metabolism were identified as deamino-metamitron and metamitron-N-glucoside. PABA, known to inhibit the deamination of metribuzin, did not alter the metabolism of metamitron, and nor did the cytochrome P450 inhibitor PBO. However, inhibition of metamitron metabolism in the presence of the cytochrome P450 inhibitor ABT was demonstrated. CONCLUSION: Metamitron metabolism in C. album may act as a basic tolerance mechanism, which can be important in circumstances favouring this degradation pathway.
Asunto(s)
Chenopodium album/metabolismo , Herbicidas/metabolismo , Triazinas/metabolismo , Adsorción , Resistencia a los Herbicidas , Ácidos Picolínicos , Butóxido de Piperonilo , Hojas de la Planta/metabolismo , Raíces de Plantas/metabolismo , TriazolesRESUMEN
Uptake rate of (133)Cs, at three different concentrations of CsCl, by Calendula alata, Amaranthus chlorostachys and Chenopodium album plants grown outdoors was studied. These plants grow abundantly in semi-arid regions and their varieties exist in many parts of the world. When exposed to lowest Cs concentration 68 percent Cs was remediated by Chenopodium album.(133)Cs accumulation in shoots of Amaranthus chlorostachys reached its highest value of 2146.2 mg kg(-1) at a (133)Cs supply level of 3.95 mg l(-1) of feed solution. The highest concentration ratio value was 4.89 for Amaranthus chlorostachys, whereas for the other tests it ranged from 0.74 to 3.33. Furthermore uptake of (133)Cs by all three species increased with increasing metal concentrations. The results also indicated that hydroponically grown Calendula alata, Amaranthus chlorostachys and Chenopodium album could be used as potential candidate plants for phytoremediation of solutions contaminated with Cs.
Asunto(s)
Amaranthus/metabolismo , Calendula/metabolismo , Cesio/metabolismo , Chenopodium album/metabolismo , Contaminantes del Suelo/metabolismo , Biodegradación Ambiental , Hidroponía , Raíces de Plantas/metabolismo , Brotes de la Planta/metabolismoRESUMEN
Plants resistant to triazine-type herbicides are known to be altered in their photosystem II reaction center. Serine at site 264 in D1 protein is replaced by glycine. The measurements of chlorophyll a fluorescence excitations with a variable number of saturating flashes in Chenopodium album plants show characteristic differences between the resistant and the wild-type plants. These differences appear in response to the first flash as well as in the rise pattern of subsequent flashes of a 12.5 Hz flash train. The differences indicate a higher concentration of Q(B)-nonreducing reaction centers in the resistant biotype, and confirm earlier results on a slower rate of electron transport between the primary and secondary electron acceptors.
Asunto(s)
Chenopodium album/metabolismo , Clorofila/metabolismo , Complejo de Proteína del Fotosistema II/metabolismo , Proteínas de Plantas/metabolismo , Triazinas/farmacología , Clorofila A , Fluorescencia , Fluorometría , Resistencia a los Herbicidas , Cinética , Hojas de la Planta/metabolismoRESUMEN
Production of heat-shock proteins (Hsps) is a key adaptation to acute heat stress and will be important in determining plant responses to climate change. Further, intraspecifc variation in Hsps, which will influence species-level response to global warming, has rarely been examined in naturally occurring plants. To understand intraspecific variation in plant Hsps and its relevance to global warming, we examined Hsp content and thermotolerance in five naturally occurring populations of Chenopodium album L. from contrasting thermal environments grown at low and high temperatures. As expected, Hsp accumulation varied between populations, but this was related more to habitat variability than to mean temperature. Unexpectedly, Hsp accumulation decreased with increasing variability of habitat temperatures. Hsp accumulation also decreased with increased experimental growth temperatures. Physiological thermotolerance was partitioned into basal and induced components. As with Hsps, induced thermotolerance decreased with increasing temperature variability. Thus, populations native to the more stressful habitats, or grown at higher temperatures, had lower Hsp levels and induced thermotolerance, suggesting a greater reliance on basal mechanisms for thermotolerance. These results suggest that future global climate change will differentially impact ecotypes within species, possibly by selecting for increased basal versus inducible thermotolerance.
Asunto(s)
Chenopodium album/metabolismo , Chenopodium album/fisiología , Efecto Invernadero , Proteínas de Choque Térmico/metabolismo , Fotosíntesis/fisiología , Temperatura , Regulación de la Expresión Génica de las PlantasRESUMEN
Antioxidant capacity, total phenolic content and flavonoid glycosides profile were compared in C.album samples grown in intensively cultivated (IC) and nondisturbed (ND) soils to evaluate differences in their nutraceutical potential. Petroleum ether, methanol, and aqueous extracts were sequentially obtained from C. album dried samples. Methanol crude extract exhibited the highest antioxidant potential and phenolic content, which were significantly enhanced by soil deterioration. This feature was enhanced in its ethyl acetate/n-buthanol subextract that also yielded higher amounts of the fraction containing flavonoid glycosides in samples grown in IC soils. Compounds were isolated by activity guided fractionation, and chemical structure-antioxidant activity relationships were established. Chemical structures were elucidated by chemical and spectroscopic methods. Six known flavonoid glycosides were isolated, and their antioxidant activity was determined by DPPH assay. 1, quercetin-3-O-(2",6"-di-O-R-L-rhamnopyranosyl)-beta-D-glucopyranoside; 2, kaempferol-3-O-(2",6"-di-O-R-L-rhamnopyranosyl)-beta-D-glucopyranoside; 3, quercetin-3-O-beta-D-glucopyranosyl-(1'''-->6")-beta-D-glucopyranoside; 4, rutin; 5, quercetin-3-O-beta-D-glucopyranoside; and 6, kaempferol-3-O-beta-D-glucopyranoside. Triosides 1 and 2 were identified for the first time in C. album. Our results suggest that this edible weed, ubiquitously present in cultivated fields, should be considered as a nutraceutical food and an alternative source for nutrients and free radical scavenging compounds, particularly when collected from cultivated fields that seem to increase some of its advantages.
Asunto(s)
Antioxidantes/química , Chenopodium album/química , Chenopodium album/metabolismo , Flavonoides/química , Suelo/análisis , Antioxidantes/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Flavonoides/aislamiento & purificación , Glicósidos/química , Glicósidos/aislamiento & purificación , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificaciónRESUMEN
The algorithm for simulation of the OJDIP fluorescence induction curve in chloroplasts under variable conditions is presented. It is derived from analyzes of chlorophyll a fluorescence kinetics upon excitation with single- (STF), twin- (TTF) and repetitive STF excitations, and from the rate equations that describe the sequence of transfer steps associated with the reduction of the primary quinone acceptor Q(A) and the release of photochemical fluorescence quenching of photosystem II (PSII) in multi-turnover excitation (MTF). The fluorescence induction algorithm (FIA) considers a photochemical O-J-D, a photo-electrochemical J-I and an I-P component (phase) which probably is associated with a photo-electric interaction between PSI and PSII. The photochemical phase incorporates the kinetics associated with the double reduction of the acceptor pair [PheQ(A)] in Q(B)-nonreducing reaction centers (RCs) and the associated doubling of the variable fluorescence, in agreement with the three-state trapping model (TSTM) of PSII. Application of and results with the algorithm are illustrated for MTF-induced OJDIP curves, measured in dark-adapted, in STF pre-excited and in DCMU inhibited thylakoids.
Asunto(s)
Algoritmos , Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo , Chenopodium album/metabolismo , Clorofila/metabolismo , Cloroplastos/metabolismo , Electroquímica , Fluorescencia , Fotoquímica , Tilacoides/metabolismoRESUMEN
Widespread contamination of California water bodies by the organophosphate insecticides diazinon and chlorpyrifos is well documented. While their usage has decreased over the last few years, a concomitant increase in pyrethroid usage (e.g., permethrin) (replacement insecticides) has occurred. Vegetated agricultural drainage ditches (VADD) have been proposed as a potential economical and environmentally efficient management practice to mitigate the effects of pesticides in irrigation and storm runoff. Three ditches were constructed in Yolo County, California for a field trial. A U-shaped vegetated ditch, a V-shaped vegetated ditch, and a V-shaped unvegetated ditch were each amended for 8 h with a mixture of diazinon, permethrin, and suspended sediment simulating an irrigation runoff event. Water, sediment, and plant samples were collected spatially and temporally and analyzed for diazinon and permethrin concentrations. Pesticide half-lives were similar between ditches and pesticides, ranging from 2.4 to 6.4 h. Differences in half-distances (distance required to reduce initial pesticide concentration by 50%) among pesticides and ditches were present, indicating importance of vegetation in mitigation. Cis-permethrin half-distances in V ditches ranged from 22 m (V-vegetated) to 50 m (V-unvegetated). Half-distances for trans-permethrin were similar, ranging from 21 m (V-vegetated) to 55 m (V-unvegetated). Diazinon half-distances demonstrated the greatest differences (55 m for V-vegetated and 158 m for V-unvegetated). Such economical and environmentally successful management practices will offer farmers, ranchers, and landowners a viable alternative to more conventional (and sometimes expensive) practices.
Asunto(s)
Diazinón/análisis , Insecticidas/análisis , Permetrina/análisis , Contaminantes Químicos del Agua/análisis , Contaminación del Agua/prevención & control , Agricultura/métodos , California , Chenopodium album/metabolismo , Diazinón/metabolismo , Drenaje de Agua/métodos , Sedimentos Geológicos/análisis , Hordeum/metabolismo , Insecticidas/metabolismo , Lolium/metabolismo , Permetrina/metabolismo , Movimientos del Agua , Contaminantes Químicos del Agua/metabolismoRESUMEN
This study has determined the uptake of three pesticides, applied as commercial or model formulations in the presence of a wide range of surfactants, into the leaves of three plant species (bentazone into Chenopodium album L. and Sinapis alba L., epoxiconazole and pyraclostrobin into Triticum aestivum L.). The results have confirmed previous findings that the initial dose (nmol mm(-2)) of xenobiotic applied to plant foliage is a strong, positive determinant of uptake. This held true for all the pesticide formulations studied, although surfactant concentration was found to have an effect. The lower surfactant concentrations studied showed an inferior relationship between the amount of xenobiotic applied and uptake. High molecular mass surfactants also produced much lower uptake than expected from the dose uptake equations in specific situations.
Asunto(s)
Plaguicidas/metabolismo , Hojas de la Planta/metabolismo , Tensoactivos/farmacología , Xenobióticos/administración & dosificación , Xenobióticos/metabolismo , Benzotiadiazinas/administración & dosificación , Benzotiadiazinas/metabolismo , Carbamatos/administración & dosificación , Carbamatos/metabolismo , Chenopodium album/efectos de los fármacos , Chenopodium album/metabolismo , Compuestos Epoxi/administración & dosificación , Compuestos Epoxi/metabolismo , Pirazoles/administración & dosificación , Pirazoles/metabolismo , Sinapis/efectos de los fármacos , Sinapis/metabolismo , Estrobilurinas , Triazoles/administración & dosificación , Triazoles/metabolismo , Triticum/efectos de los fármacos , Triticum/metabolismoRESUMEN
The objective of this study was to determine whether a logistic-kinetic penetration model could be applied to whole plant uptake. Uptake over 24 h was determined for three model compounds, applied in the presence and absence of surfactants, into the leaves of two plant species. Data for two time intervals were used in the model to predict uptake at intermediate intervals and compared with experimental results. Overall, the model fit the whole plant uptake data well. The study confirmed that an increase (or decrease) in active ingredient (ai) concentration or an increase in contact area will have no effect on the penetration rate factor, q, within the normal working concentration range. This enabled uptake to be predicted at different times for concentrations of ai not already studied, having first derived q for one concentration of the formulation of interest and having 24 h (maximum) uptake results for all formulations and concentrations of interest. The advantages of the models and equations described are that few variables are required, and they are simple to measure.