Linking jasmonates with vitamin E accumulation in plants: a case study in the Mediterranean shrub Cistus albidus L.

MAIN CONCLUSION: Jasmonic acid positively modulates vitamin E accumulation, but the latter can also partly influence the capacity to accumulate the jasmonic acid precursor, 12-oxo-phytodienoic acid, in white-leaved rockrose (Cistus albidus L.) plants growing in their natural habitat. This study suggests a bidirectional link between chloroplastic antioxidants and lipid peroxidation-derived hormones in plants. While vitamin E is well known for its antioxidant properties being involved in plant responses to abiotic stress, jasmonates are generally related to biotic stress responses in plants. Studying them in non-model plants under natural conditions is crucial for the knowledge on their relationship, which will help us to better understand mechanisms and limits of stress tolerance to implement better conservation strategies in vulnerable ecosystems. We studied a typical Mediterranean shrub, white-leaved rockrose (Cistus albidus) under natural conditions during three winters and we analyzed both α and γ-tocopherol, and the three main jasmonates forms 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA), and jasmonoyl-isoleucine (JA-Ile). We found that JA contents positively correlated with vitamin E accumulation, most particularly with γ-tocopherol, the precursor of α-tocopherol (the most active vitamin E form). This finding was confirmed by exogenous application of methyl jasmonate (MeJA) in leaf discs under controlled conditions, which increased γ-tocopherol when applied at 0.1 mM MeJA and α-tocopherol at 1 mM MeJA. Furthermore, a complementary meta-analysis study with previously published reports revealed a positive correlation between JA and vitamin E, although this relationship turned to be strongly species specific. A strong negative correlation was observed, however, between total tocopherols and OPDA (a JA precursor located in chloroplasts). This antagonistic effect was observed between α-tocopherol and OPDA, but not between γ-tocopherol and OPDA. It is concluded that (i) variations in jasmonates and vitamin E due to yearly, inter-individual and sun orientation-driven variability are compatible with a partial regulation of vitamin E accumulation by jasmonates, (ii) vitamin E may also exert a role in the modulation of the biosynthesis of OPDA, with a much smaller effect, if any, on other jasmonates, and (iii) a trade-off in the accumulation of vitamin E and jasmonates might occur in the regulation of biotic and abiotic stress responses in plants.

Physiological response of Cistus salviifolius L. to high arsenic concentrations.

Arsenic is a trace element found in the environment which can be particularly toxic to living organisms. However, some plant species such as those of the genus Cistus are able to grow in soils with high As concentrations and could be used in the sustainable rehabilitation of mining areas through phytostabilization. In this work, the growth and the physiological response of Cistus salviifolius L. to As-induced oxidative stress at several concentrations (reaching 30 mg L-1) in an hydroponic system were evaluated for 30 days. Several growth parameters, chlorophyll content, chemical composition, one indicator of oxidative stress (H2O2) and two of the major antioxidative metabolites (ascorbate and glutathione) were analysed. The toxic effect of As was better perceived in the plants submitted to treatments with concentrations of 20 and 30 mg As L-1. Plants subjected to these treatments had higher concentration of As in roots and shoots. The concentrations of Ca, Mg, K and Fe in the plants as well as a large part of the evaluated growth parameters were also affected. Arsenic did not interfere with the ability of the plant to perform photosynthesis, as there were no significant differences in the contents of chlorophyll a, b and total between the different treatments. Plants from all treatments accumulated higher amount of As in roots than in shoots, and it was also in the roots that the concentrations of H2O2, AsA and GSH were higher. Cistus salviifolius showed high tolerance to As up to the concentration of 5 mg L-1, which makes it a species with high potential to be used in the phytostabilization of soils contaminated with As and presenting high concentrations of the element in the soil solution.

Unraveling the crucial role of the ascorbate-glutathione cycle in the resilience of Cistus monspeliensis L. to withstand high As concentrations.

Cistus monspeliensis L. is a species that grows spontaneously in contaminated mining areas of the Iberian Pyrite Belt. This species can accumulate high concentrations of As in the shoots without visible signs of phytotoxicity. In order to understand the physiological mechanisms underlying this tolerance, C. monspeliensis was grown in an Arenosol irrigated with aqueous nutrient solutions containing increasing concentrations of As (0, 1500, 5000, 10000, 15000 µM) and the effects of this metalloid on plant development and on the defence mechanisms against oxidative stress were monitored. Independently of the treatment, As was mainly retained in the roots. The plants with the highest concentrations of As in the shoots (> 5000 µM) showed toxicity symptoms such as chlorosis, low leaf size and decrease in biomass production and also nutritional deficiencies. Most of the studied physiological parameters (pigments, glutathione, ascorbate and antioxidative enzymes) showed significant correlation with As concentration in roots and shoots. Pigments, especially anthocyanins, were negatively affected even in the treatments with the lowest As concentrations. Glutathione increased significantly in roots at low As levels while in shoots this increase occurred in all As treatments. Ascorbate decreased in both tissues with As addition. The highest concentrations of As in shoots of C. monspeliensis triggered defence mechanisms against oxidative stress, namely by inducing the expression of genes coding antioxidative enzymes.

The physiological mechanisms underlying the ability of Cistus monspeliensis L. from São Domingos mine to withstand high Zn concentrations in soils.

Cistus monspeliensis L. is a species that grows spontaneously in contaminated mining areas from the Iberian Pyrite Belt. This species can have high concentrations of Zn in the shoots without visible signs of phytotoxicity. In order to understand the physiological mechanisms underlying this tolerance, C. monspeliensis was grown at several concentrations of Zn(2+) (0, 500, 1000, 1500, 2000µM) and the effects of this metal on plant development and on the defence mechanisms against oxidative stress were evaluated. Independently of the treatment, Zn was mainly retained in the roots. The plants with the highest concentrations of Zn showed toxicity symptoms such as chlorosis, low leaf size and decrease in biomass production. At 2000µM of Zn, the dry biomass of the shoots decreased significantly. High concentrations of Zn in shoots did not induce deficiencies of other nutrients, except Cu. Plants with high concentrations of Zn had low amounts of chlorophyll, anthocyanins and glutathione and high contents of H2O2. The highest concentrations of Zn in shoots of C. monspeliensis triggered defence mechanisms against oxidative stress, namely by triggering antioxidative enzyme activity and by direct reactive oxygen species (ROS) scavenging through carotenoids, that are unaffected by stress due to stabilisation by ascorbic acid.

Zeatin modulates flower bud development and tocopherol levels in Cistus albidus (L.) plants as they age.

In a previous study we showed that Cistus albidus (L.) experiences an age-dependent decay in flower vigour correlated with a decline in trans-zeatin (tZ) levels. In the present study we aimed to establish a causal relationship between these two phenomena. Exogenous tZ applied to plants grown under semi-controlled conditions did not rescue flower vigour; however, it accelerated flower development, but only in younger individuals. Older plants showed lower tocopherol levels in flower buds, which were restored by exogenous tZ, suggesting that a loss of antioxidant defences may underlie the age-dependent decay in flower vigour. We conclude that declining tZ levels may not be directly responsible for the age-associated loss of floral vigour; that tZ modulates the speed of flower development as plants age; and that flower buds alter their sensitivity to tZ as plants age.