RESUMEN
In this study, we investigated the beneficial effects of grapefruit IntegroPectin, derived from industrial waste grapefruit peels via hydrodynamic cavitation, on microglia cells exposed to oxidative stress conditions. Grapefruit IntegroPectin fully counteracted cell death and the apoptotic process induced by cell exposure to tert-butyl hydroperoxide (TBH), a powerful hydroperoxide. The protective effects of the grapefruit IntegroPectin were accompanied with a decrease in the amount of ROS, and were strictly dependent on the activation of the phosphoinositide 3-kinase (PI3K)/Akt cascade. Finally, IntegroPectin treatment inhibited the neuroinflammatory response and the basal microglia activation by down-regulating the PI3K- nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)- inducible nitric oxide synthase (iNOS) cascade. These data strongly support further investigations aimed at exploring IntegroPectin's therapeutic role in in vivo models of neurodegenerative disorders, characterized by a combination of chronic neurodegeneration, oxidative stress and neuroinflammation.
Asunto(s)
Citrus paradisi , Microglía , Humanos , Microglía/metabolismo , Citrus paradisi/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Antiinflamatorios/farmacología , Antiinflamatorios/metabolismo , Línea CelularRESUMEN
Light, a crucial environmental signal, is involved in the regulation of secondary metabolites. To understand the mechanism by which light influences carotenoid metabolism, grapefruits were bagged with four types of light-transmitting bags that altered the transmission of solar light. We show that light-transmitting bagging induced changes in carotenoid metabolism during fruit ripening. Compared with natural light, red light (RL)-transmittance treatment significantly increases the total carotenoid content by 62%. Based on weighted gene co-expression network analysis (WGCNA), 'blue' and 'turquoise' modules are remarkably associated with carotenoid metabolism under different light treatment (p < 0.05). Transcriptome analysis identifies transcription factors (TFs) bHLH128, NAC2-like/21/72, MYB-like, AGL11/AGL61, ERF023/062, WRKY20, SBPlike-7/13 as being involved in the regulation of carotenoid metabolism in response to RL. Under RL treatment, these TFs regulate the accumulation of carotenoids by directly modulating the expression of carotenogenic genes, including GGPPS2, PDS, Z-ISO, ZDS2/7, CRTISO3, CYP97A, CHYB, ZEP2, CCD1-2. Based on these results, a network of the regulation of carotenoid metabolism by light in citrus fruits is preliminarily proposed. These results show that RL treatments have great potential to improve coloration and nutritional quality of citrus fruits.
Asunto(s)
Citrus paradisi , Carotenoides/metabolismo , Citrus paradisi/metabolismo , Frutas/genética , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas , LuzRESUMEN
BACKGROUND: Numerous reports show that herbal medicines can be utilized in the treatment of different liver disorders. In this study, antioxidant, antibacterial, and anticancer activities of individual as well as combined 80% ethanolic extracts of Artemisia absinthium leaves and Citrus paradisi peels were investigated. METHODS AND RESULTS: Values of total phenolic contents (TPC), total flavonoid contents (TFC), DPPH-radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured to explore the antioxidant capacity. To assess antibacterial activity, four bacterial strains (Escherichia coli, Staphylococcus aureus, Salmonella enterica, and Klebsiella pneumoniae) were used. Anticancer activity was assessed on Huh-7 (liver cancer) and Vero (non-cancerous) cell lines. FRAP activity of combined plants extract was higher as compared to their individual effect; the trend did not hold in the case of DPPH-radical scavenging activity. Antibacterial activity of combined extracts by disk diffusion method was observed only against E.coli. MTT results indicated that both plants had a cytotoxic effect on Huh-7 cell line but did not show any effect on Vero cell line. Our data showed a strong negative correlation between the amount of TPC, TFC, & DPPH radicals-scavenging activity and viability of Huh-7 cell line.However, no effect was shown on the non-cancerous cell line. CONCLUSION: The ethanolic extracts of Artemisia absinthium leaves and Citrus paradisi peels can be used against liver cancer because of their antioxidant, antibacterial, and anticancer activities.
Asunto(s)
Artemisia absinthium/enzimología , Citrus paradisi/enzimología , Neoplasias Hepáticas/tratamiento farmacológico , Antibacterianos/farmacología , Antioxidantes/farmacología , Artemisia absinthium/metabolismo , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Citrus paradisi/metabolismo , Flavonoides/farmacología , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patología , Fenoles/análisis , Extractos Vegetales/farmacología , Hojas de la Planta/químicaAsunto(s)
Antipsicóticos/farmacocinética , Trastorno Bipolar/tratamiento farmacológico , Citrus paradisi/metabolismo , Inhibidores del Citocromo P-450 CYP3A/metabolismo , Interacciones Alimento-Droga , Jugos de Frutas y Vegetales , Fumarato de Quetiapina/farmacocinética , Adulto , Citrus paradisi/efectos adversos , Sobredosis de Droga/diagnóstico , Femenino , Jugos de Frutas y Vegetales/efectos adversos , HumanosRESUMEN
Plants produce â¼300 aromatic compounds enzymatically linked to prenyl side chains via C-O bonds. These O-prenylated aromatic compounds have been found in taxonomically distant plant taxa, with some of them being beneficial or detrimental to human health. Although their O-prenyl moieties often play crucial roles in the biological activities of these compounds, no plant gene encoding an aromatic O-prenyltransferase (O-PT) has been isolated to date. This study describes the isolation of an aromatic O-PT gene, CpPT1, belonging to the UbiA superfamily, from grapefruit (Citrus × paradisi, Rutaceae). This gene was shown responsible for the biosynthesis of O-prenylated coumarin derivatives that alter drug pharmacokinetics in the human body. Another coumarin O-PT gene encoding a protein of the same family was identified in Angelica keiskei, an apiaceous medicinal plant containing pharmaceutically active O-prenylated coumarins. Phylogenetic analysis of these O-PTs suggested that aromatic O-prenylation activity evolved independently from the same ancestral gene in these distant plant taxa. These findings shed light on understanding the evolution of plant secondary (specialized) metabolites via the UbiA superfamily.
Asunto(s)
Angelica/genética , Citrus paradisi/genética , Evolución Molecular , Furocumarinas/biosíntesis , Proteínas de Plantas/genética , Prenilación , Angelica/metabolismo , Citrus paradisi/metabolismo , Filogenia , Proteínas de Plantas/metabolismoRESUMEN
Intestinal cytochrome P450 3A (CYP3A) plays an important role in oral drug metabolism, but only endogenous metabolic markers for measuring hepatic CYP3A activity were identified. Our study evaluated whether hepatic CYP3A markers reflected intestinal CYP3A activity. An open-label, three-period, six-treatment, one-sequence clinical trial was performed in 16 healthy Korean males. In the control phase, all subjects received a single dose of intravenous (IV) and oral midazolam (1 mg and 5 mg, respectively). Clarithromycin (500 mg) was administered twice daily for 4 days to inhibit hepatic and intestinal CYP3A, and 500 mL of grapefruit juice was given to inhibit intestinal CYP3A. Clarithromycin significantly inhibited total CYP3A activity, and the clearance of IV and apparent clearance of oral midazolam decreased by 0.15- and 0.32-fold, respectively. Grapefruit juice only reduced the apparent clearance of oral midazolam by 0.84-fold, which indicates a slight inhibition of intestinal CYP3A activity. Urinary markers, including 6ß-OH-cortisol/cortisol and 6ß-OH-cortisone/cortisone, were significantly decreased 0.5-fold after clarithromycin administration but not after grapefruit juice. The fold changes in 6ß-OH-cortisol/cortisol and 6ß-OH-cortisone/cortisone did not correlate to changes in intestinal availability but did correlate to hepatic availability. In conclusion, endogenous metabolic markers are only useful to measure hepatic, but not intestinal, CYP3A activity.
Asunto(s)
Citrus paradisi/metabolismo , Claritromicina/orina , Citocromo P-450 CYP3A/orina , Mucosa Intestinal/metabolismo , Hígado/metabolismo , Midazolam/orina , Administración Intravenosa , Administración Oral , Adulto , Biomarcadores/sangre , Biomarcadores/orina , Claritromicina/administración & dosificación , Claritromicina/sangre , Citocromo P-450 CYP3A/sangre , Citocromo P-450 CYP3A/genética , Interacciones Alimento-Droga/fisiología , Voluntarios Sanos , Humanos , Mucosa Intestinal/efectos de los fármacos , Hígado/efectos de los fármacos , Masculino , Tasa de Depuración Metabólica/efectos de los fármacos , Tasa de Depuración Metabólica/fisiología , Midazolam/administración & dosificación , Midazolam/sangreRESUMEN
An HS-SPME GC-MS analysis of the volatile compounds adsorbed at the outer surface of lemon and grapefruit pectins obtained via the hydrodynamic cavitation of industrial waste streams of lemon and grapefruit peels in water suggests important new findings en route to understanding the powerful and broad biological activity of these new pectic materials. In agreement with the ultralow degree of esterification of these pectins, the high amount of highly bioactive α-terpineol and terpinen-4-ol points to limonene (and linalool) decomposition catalyzed by residual citric acid in the citrus waste peel residue of the juice industrial production.
Asunto(s)
Citrus paradisi/química , Citrus/química , Compuestos Orgánicos Volátiles/química , Vías Biosintéticas , Citrus/metabolismo , Citrus paradisi/metabolismo , Frutas/química , Cromatografía de Gases y Espectrometría de Masas , Estructura Molecular , Fitoquímicos/análisis , Fitoquímicos/química , Compuestos Orgánicos Volátiles/análisis , Compuestos Orgánicos Volátiles/metabolismoRESUMEN
Coumarins are plant-derived secondary metabolites. The crystal structure of three coumarins-bergamottin, osthole and fraxidin-are described and we analyze intermolecular interactions and their role in crystal formation. Bergamottin is a furanocoumarin found in citrus plants, which is a strong inhibitor of the principal human metabolizing enzyme, cytochrome P450 3A4 (CYP3A4). The crystal structure determinations of three coumarins give us the geometrical parameters and reveal the parallel-displaced π-π stacking and hydrogen bonding intermolecular interactions used for molecular assembly in the crystal structure. A quite strong (less than 3.4 Å) stacking interaction of bergamottin appears to be a determining feature that distinguishes it from other coumarins studied in this work. Our DFT computational studies on the three natural products of the same coumarin family docked into the active site of CYP3A4 (PDB 4D78) show different behavior for these coumarins at the active site. When the substrate is bergamottin, the importance of π-π stacking and hydrogen bonding, which can anchor the substrate in place, appears fundamental. In contrast, fraxidin and osthole show carbonyl coordination to iron. Our docking calculations show that the bergamottin tendency towards π-π stacking is important and likely influences its interactions with the heme group of CYP3A4.
Asunto(s)
Citrus paradisi/metabolismo , Cumarinas/química , Cumarinas/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Furocumarinas/metabolismo , Dominio Catalítico/fisiología , Citrus paradisi/química , Cristalografía por Rayos X/métodos , Furocumarinas/química , Hemo/química , Hemo/metabolismo , Humanos , Enlace de HidrógenoRESUMEN
Structure and properties of pectin can be affected by extraction methods. In this study, grapefruit peel pectins extracted by HCl (at pH 1 [P1], 2 [P2], and 3 [P3]) and NaOH (at pH 9 [P9], 10 [P10], and 11 [P11]) were prepared and characterized. Atomic force microscopy (AFM) provided direct evidence of complex nano-structural patterns of pectins and revealed cross-linked networks of P10 and P11. Small-angle X-ray scattering (SAXS) demonstrated that P1, P2, and P3 possessed a relatively extended conformation, whereas P9, P10, and P11 displayed a three-dimensional structure and folded conformation. The compact and extended conformations of P3 contributed to its high viscosity in solution and the stability of the formed emulsion (75%). Porous surface and larger three-dimensional nanostructure (Dmax: 23 nm) of P10 facilitated its ion-binding capacity. Our results provide valuable insight into relationship between extraction methods and structure-properties of pectin, facilitating design of functional pectins.
Asunto(s)
Citrus paradisi/metabolismo , Ácido Clorhídrico/química , Pectinas/química , Hidróxido de Sodio/química , Emulsiones/química , Frutas/metabolismo , Concentración de Iones de Hidrógeno , Nanoestructuras/química , Extractos Vegetales/metabolismo , Porosidad , Reología , Dispersión del Ángulo Pequeño , Viscosidad , Difracción de Rayos XRESUMEN
Deep eutectic solvents (DES) and aqueous glycerol were proposed as green alternatives to conventional solvents for the extraction of polyphenols from grapefruit peels. In order to increase the extraction kinetics and yields of polyphenols, high voltage electrical discharges (HVED) were used as a pre-treatment technology (energy varied between 7.27 and 218â¯kJ/kg). Results showed that the HVED energy input can be reduced, when the subsequent solid-liquid extraction was performed in 20% (w/v) aqueous glycerol or in DES (lactic acid: glucose) instead of water. The addition of glycerol has reduced the energy of the pre-treatment by 6 times. The same diffusivity of polyphenols (4â¯×â¯10-11â¯m2/s) was obtained in water from HVED pre-treated peels at 218â¯kJ/kg and in aqueous glycerol from pre-treated peels at 36â¯kJ/kg. The solubility of naringin, the main flavonoid compound of grapefruit peels in the solvents, was investigated through a theoretical modelling of its Hansen solubility parameters.
Asunto(s)
Citrus paradisi/química , Glicerol/química , Polifenoles/aislamiento & purificación , Solventes/química , Cromatografía Líquida de Alta Presión , Citrus paradisi/metabolismo , Electricidad , Flavanonas/química , Flavonoides/química , Glucosa/química , Ácido Láctico/química , Extracción Líquido-Líquido , Polifenoles/análisis , Solubilidad , Agua/químicaRESUMEN
Citrus pectin hydrolysates (Citrus paradisi [Mafc.]) from "Foster," "Red Shambar," "Tangelo Orlando," and "Citrumelo Swingle" cultivars were obtained by partial chemical hydrolysis and their properties as culture media (sole carbon/nutrient source) and encapsulating agents of Lactobacillus plantarum CIDCA 83114 were evaluated. The concentration of neutral sugars was maximal after 2-hour hydrolysis. All hydrolysates were rich in glucose >xylose >galactose >galacturonic acid >mannose >arabinose. "Citrumelo Swingle" cultivar was the one with the highest concentration of xylose. After 24 hr of fermentation with L. plantarum CIDCA 83114, bacterial viability increased from 6.76 ± 0.14 to almost 9 log CFU/mL, and lactic acid concentration, from 2.63 ± 0.41 to 7.82 ± 0.15 mmol/L in all hydrolysates. Afterwards, bacteria were entrapped in pectate-calcium beads by ionotropic gelation. Bacterial viability did not significantly decrease after freeze-drying and storage the beads at 4 °C for 45 days. PRACTICAL APPLICATION: Pectin hydrolysates were adequate culture media for microorganisms, as determined by the viabililty and lactic acid production. Considering that citrus peels are agro-wastes obtained in large quantities, their use as encapsulating materials provides a solution to overcome the environmental problem they entail.
Asunto(s)
Citrus paradisi/química , Medios de Cultivo/metabolismo , Lactobacillus plantarum/química , Pectinas/química , Citrus paradisi/metabolismo , Medios de Cultivo/química , Fermentación , Liofilización , Hidrólisis , Ácido Láctico/análisis , Ácido Láctico/metabolismo , Lactobacillus plantarum/clasificación , Lactobacillus plantarum/crecimiento & desarrollo , Lactobacillus plantarum/metabolismo , Pectinas/metabolismo , Azúcares/análisis , Azúcares/metabolismoRESUMEN
4ß-Hydroxycholesterol (4ßOHC) is an endogenous CYP3A4 metabolite. However, it is unclear whether circulating levels of 4ßOHC may reflect hepatic CYP3A4 activity or both hepatic and intestinal enzyme activity. The aim of this study was to investigate the effect of grapefruit juice, regarded to be a selective intestinal CYP3A4 inhibitor, on serum 4ßOHC levels in healthy volunteers. The participants (n = 22) consumed grapefruit juice twice daily for 3 weeks followed by a 2-week washout period. Blood samples for measurements of 4ßOHC and the non-CYP3A4-derived oxysterols 24-hydroxycholesterol (24OHC) and 27-hydroxycholesterol (27OHC), as well as lathosterol and total cholesterol, were drawn on days 0, 7, 21, and 35. Median individual changes (ratios) in cholesterol-corrected 4ßOHC levels from baseline to weeks 1, 3, and 5 were 0.94 (P = 0.2), 0.98 (P = 0.3), and 0.97 (P = 0.9), respectively. In comparison, median changes (ratios) in cholesterol-corrected levels of 24OHC at the same points were 1.01 (P = 0.6), 0.98 (P = 0.3), and 0.99 (P = 0.5), and of 27OHC 1.01 (P = 0.8), 0.97 (P = 0.5), and 0.99 (P = 0.2). Surprisingly, serum concentration of cholesterol was significantly reduced by approximately 5% after 1 week (P = 0.03), while median cholesterol-corrected levels of lathosterol increased significantly and persistently by approximately 15% during the whole 5-week period (P < 0.04). In conclusion, the present findings suggest that intestinal CYP3A4 is not relevant for the overall formation of 4ßOHC in healthy volunteers. The fact that grapefruit juice altered cholesterol homeostasis should be further investigated.
Asunto(s)
Citrus paradisi/química , Citocromo P-450 CYP3A/metabolismo , Jugos de Frutas y Vegetales/efectos adversos , Hidroxicolesteroles/sangre , Preparaciones Farmacéuticas/metabolismo , Biomarcadores/sangre , Índice de Masa Corporal , Colesterol/sangre , Citrus paradisi/metabolismo , Femenino , Interacciones Alimento-Droga , Semivida , Voluntarios Sanos , Humanos , Intestinos/enzimología , Hígado/enzimología , Masculino , Factores Sexuales , Adulto JovenRESUMEN
This study was to evaluate the effect of graft copolymer (CTS-g-SA) of chitosan (CTS) and salicylic acid (SA) on the storability of grapefruit fruits during postharvest storage. Results indicate that the graft copolymer treatment significantly depressed green mold caused by Penicillium digitatum. The graft copolymer application kept fruit firmness without impairing the fruit quality. Moreover, the graft copolymer treatment inhibited the activity and gene expression of cell wall-modifying enzymes such as polygalacturonase, cellulase, pectin methylesterase, α-l-arabinofuranosidase, ß-galactosidase, and suppressed the modification of cell wall components including covalently bound polysaccharide (sodium carbonate soluble pectin, 24% KOH-soluble fraction), which were associated with fruit softening. These results suggested that graft copolymer application can be recognized as a postharvest technique to suppress rotting and delay softening through inhibiting solubilization of cell wall polysaccharides.
Asunto(s)
Pared Celular/efectos de los fármacos , Quitosano/química , Citrus paradisi/metabolismo , Polímeros/química , Ácido Salicílico/química , Pared Celular/metabolismo , Celulosa/metabolismo , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Pectinas/metabolismo , Poligalacturonasa/metabolismo , Polímeros/farmacologíaRESUMEN
Nutrient deficiency has economic and ecological repercussions for citrus fruit crops worldwide. Citrus crops rely on fertilization to maintain good fruit output and quality, whereas new crop management policy aims to reduce fertilizers input. New rootstocks are needed to meet to this constraint, and the use of new tetraploid rootstocks better adapted to lower nutrient intake could offer a promising way forward. Here we compared physiological, biochemical and anatomic traits of leaves in diploid (2x) and doubled-diploid (4x) Citrumelo 4475 (Citrus paradisi L. Macf.â¯×â¯Poncirus trifoliata L. Raf.) and Volkamer lemon (Citrus limonia Osb.) seedlings over 7 months of nutrient deficiency. Photosynthetic parameters (Pnet, Gs and Fv/Fm) decreased, but to a lesser extent in 4x genotypes than 2x. Degradation of the ultrastructural organelles (chloroplasts and mitochondria) and compound cells (thylakoids and starches) was also lower in 4x genotypes, suggesting that tetraploidy may enhance tolerance to nutrient deficiency. However, leaf surface (stomata, stomatal density and epithelial cells) showed no nutrient deficiency-induced change. In 4x Citrumelo 4475, the higher tolerance to nutrient deficiency was associated with a lower MDA and H2O2 accumulation than in the 2x, suggesting a more efficient antioxidant system in the 4x genotype. However, few differences in antioxidant system and oxidative status were observed between 2x and 4x Volkamer lemons.
Asunto(s)
Citrus/genética , Diploidia , Plantones/genética , Tetraploidía , Clorofila A/metabolismo , Cloroplastos/ultraestructura , Citrus/metabolismo , Citrus/fisiología , Citrus/ultraestructura , Citrus paradisi/genética , Citrus paradisi/metabolismo , Citrus paradisi/fisiología , Citrus paradisi/ultraestructura , Microscopía Electrónica de Rastreo , Mitocondrias/ultraestructura , Nutrientes/deficiencia , Fotosíntesis , Poncirus/genética , Poncirus/metabolismo , Poncirus/fisiología , Poncirus/ultraestructura , Plantones/metabolismo , Plantones/fisiología , Plantones/ultraestructura , Estrés FisiológicoRESUMEN
BACKGROUND/AIMS: Multidrug resistance (MDR) is the most common cause of chemotherapy failure. Upregulation of P-glycoprotein (P-gp) is one of the main mechanisms underlying MDR. METHODS: In this study, we developed a targeted drug and small interfering (si)RNA co-delivery system based on specific aptamer-conjugated grapefruit-derived nanovectors (GNVs) that we tested in MDR LoVo colon cancer cells. The internalization of nanovectors in cancer cells was tested by fluorescence microscopy and flow cytometry. The anti-cancer activity in vitro was determined by colony formation and cell apoptosis assays. The biodistribution of nanovectors was analyzed by live imaging and the anti-cancer activity in vivo was observed. RESULTS: GNVs loaded with aptamer increased doxorubicin (Dox) accumulation in MDR LoVo cells, an effect that was abolished by pretreatment with DNase. The LA1 aptamer effectively promoted nanovector internalization into cells at 4°C and increased the targeted delivery of Dox to tumors. Constructs harboring Dox, LA1, and P-gp siRNA more effectively inhibited proliferation and enhanced apoptosis in cultured MDR LoVo cells while exhibiting more potent anti-tumor activity in vivo than free Dox or GNVs loaded with Dox alone or in conjunction with LA1, an effect that was associated with downregulation of P-gp expression. CONCLUSION: This GNV-based system may be an effective strategy for overcoming MDR in clinical settings.
Asunto(s)
Aptámeros de Nucleótidos/química , Doxorrubicina/química , Portadores de Fármacos/química , Nanoestructuras/química , ARN Interferente Pequeño/metabolismo , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/antagonistas & inhibidores , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/genética , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/metabolismo , Animales , Antineoplásicos/química , Antineoplásicos/farmacocinética , Antineoplásicos/uso terapéutico , Línea Celular Tumoral , Citrus paradisi/química , Citrus paradisi/metabolismo , Neoplasias del Colon/tratamiento farmacológico , Neoplasias del Colon/patología , Regulación hacia Abajo , Doxorrubicina/farmacocinética , Doxorrubicina/uso terapéutico , Resistencia a Antineoplásicos , Humanos , Ratones , Ratones Endogámicos BALB C , Ratones SCID , Distribución TisularRESUMEN
Biodegradable pH-responsive polysuccinimide nanoparticles (PSI-NPs) are synthesized for directly delivering agrochemicals to plant phloem to improve their efficacy. The PSI-NPs have an average size of 20.6 nm with negative charge on the surface. The desired responsiveness to changes in pH is demonstrated by release efficiency of the model molecule (Coumarin 6), which increases with increasing pH over 24 h. The internalization of PSI-NPs into grapefruit cells occurs in 10 min, and into nucleus in 2 h, with most of the PSI-NPs being distributed in cytoplasm and nucleus. The proportion of PSI-NPs in plant cells significantly increases with time, from 19.1% at 10 min to 55.5% at 2 h of administering. The PSI-NPs do not show significant inhibitory effects on soil microbial growth and activity. These results indicate that this smart nanodelivery system has potential of application in agriculture for mitigating phloem-limited diseases, such as citrus huanglongbing.
Asunto(s)
Ácido Aspártico/análogos & derivados , Citrus paradisi/efectos de los fármacos , Portadores de Fármacos/síntesis química , Nanopartículas/química , Péptidos/química , Células Vegetales/efectos de los fármacos , Agroquímicos/química , Agroquímicos/farmacología , Ácido Aspártico/química , Carga Bacteriana/efectos de los fármacos , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Núcleo Celular/ultraestructura , Células Cultivadas , Citrus paradisi/citología , Citrus paradisi/metabolismo , Cumarinas/química , Citoplasma/efectos de los fármacos , Citoplasma/metabolismo , Citoplasma/ultraestructura , Composición de Medicamentos/métodos , Liberación de Fármacos , Humanos , Concentración de Iones de Hidrógeno , Interacciones Hidrofóbicas e Hidrofílicas , Cinética , Tamaño de la Partícula , Floema/citología , Floema/efectos de los fármacos , Floema/metabolismo , Células Vegetales/metabolismo , Microbiología del Suelo , Electricidad Estática , Tiazoles/químicaRESUMEN
BACKGROUND: This study was performed to clarify the strategies of Penicillium digitatum during pathogenesis on citrus, assessing, on albedo plugs, the effects of treatment with sodium bicarbonate (NaHCO3 ), at two different pH values (5 and 8.3), on cell-wall-degrading enzyme activity over a period of 72 h. RESULTS: Treatment with NaHCO3 , under alkaline pH, delayed the polygalacturonase activity for 72 h, or 48 h in the case of the pectin lyase, compared with the control or the same treatment at pH 5. In contrast, pectin methyl esterase activity rapidly increased after 24 h, in plugs dipped in the same solution. In this case, the activity remained higher than untreated or pH 5-treated plugs up to 72 h. CONCLUSION: The rapid increase in pectin methyl esterase activity under alkaline conditions is presumably the strategy of the pathogen to lower the pH, soon after the initiation of infection, in order to restore an optimal environment for the subsequent polygalacturonase and pectin lyase action. In fact, at the same time, a low pH delayed the enzymatic activity of polygalacturonase and pectin lyase, the two enzymes that actually cleave the α-1,4-linkages between the galacturonic acid residues. © 2018 Society of Chemical Industry.
Asunto(s)
Pared Celular/microbiología , Citrus paradisi/microbiología , Inhibidores Enzimáticos/química , Proteínas Fúngicas/química , Penicillium/enzimología , Enfermedades de las Plantas/microbiología , Bicarbonato de Sodio/química , Pared Celular/metabolismo , Citrus paradisi/metabolismo , Inhibidores Enzimáticos/farmacología , Proteínas Fúngicas/metabolismo , Concentración de Iones de Hidrógeno , Penicillium/efectos de los fármacos , Poligalacturonasa/química , Poligalacturonasa/metabolismo , Polisacárido Liasas/química , Polisacárido Liasas/metabolismo , Bicarbonato de Sodio/farmacologíaRESUMEN
Candidatus Liberibacter asiaticus (CLas) is the presumed causal agent of Huanglongbing, one of the most destructive diseases in citrus. However, the lipid metabolism component of host response to this pathogen has not been investigated well. Here, metabolic profiling of a variety of long-chain fatty acids and their oxidation products was first performed to elucidate altered host metabolic responses of disease. Fatty acid signals were found to decrease obviously in response to disease regardless of cultivar. Several lipid oxidation products strongly correlated with those fatty acids were also consistently reduced in the diseased group. Using a series of statistical methods and metabolic pathway mapping, we found significant markers contributing to the pathological symptoms and identified their internal relationships and metabolic network. Our findings suggest that the infection of CLas may cause the altered metabolism of long-chain fatty acids, possibly leading to manipulation of the host's defense derived from fatty acids.
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Citrus/metabolismo , Citrus/microbiología , Ácidos Grasos/metabolismo , Enfermedades de las Plantas/microbiología , Rhizobiaceae , Citrus paradisi/metabolismo , Citrus paradisi/microbiología , Citrus sinensis/metabolismo , Citrus sinensis/microbiología , Florida , Peroxidación de Lípido , Metabolómica , Hojas de la Planta/metabolismo , Hojas de la Planta/microbiologíaRESUMEN
The aims of this study were (1) to investigate the effects of atorvastatin (10 mg, therapeutic dose) and grapefruit juice (GFJ), inhibitors of OATP2B1, on the pharmacokinetics of substrates for OATP2B1 and BCRP under oral small-dosing conditions (300 µg sulfasalazine, 250 µg rosuvastatin, 300 µg glibenclamide, 1200 µg celiprolol, and 600 µg sumatriptan), and (2) to evaluate the contribution of SLCO2B1*3 and ABCG2 c.421C>A polymorphisms to the pharmacokinetics of the 5 test drugs in 23 healthy volunteers. In the 3 phases, the test drugs were administered to volunteers with either water (control phase), atorvastatin, or GFJ. GFJ but not atorvastatin reduced the exposure of the test drugs significantly more than the control phase, suggesting that all 5 test drugs are substrates for OATP2B1. The SLCO2B1*3 genotype had no effect on the pharmacokinetics of the test drugs. In contrast, the exposure of sulfasalazine and rosuvastatin was significantly higher in ABCG2 421C/A than in ABCG2 421C/C individuals at all 3 phases, even under small-dosing conditions.
Asunto(s)
Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2/metabolismo , Atorvastatina/farmacocinética , Citrus paradisi/metabolismo , Transportadores de Anión Orgánico/metabolismo , Farmacogenética/métodos , Subfamilia B de Transportador de Casetes de Unión a ATP/metabolismo , Adulto , Atorvastatina/química , Atorvastatina/metabolismo , Celiprolol/química , Celiprolol/farmacocinética , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Femenino , Interacciones Alimento-Droga , Genotipo , Gliburida/química , Gliburida/farmacocinética , Humanos , Absorción Intestinal , Masculino , Proteínas de Neoplasias/metabolismo , Rosuvastatina Cálcica/química , Rosuvastatina Cálcica/farmacocinética , Sulfasalazina/química , Sulfasalazina/farmacocinética , Sumatriptán/química , Sumatriptán/farmacocinéticaRESUMEN
The complexity of the metabolic changes in obese individuals still presents a challenge for the understanding of obesity-related metabolic disruptions and for obesity management. In this study, a gas chromatography mass spectrometry (GC-MS) based metabolomics approach targeting urine metabolism has been applied to assess the potential roles of functional foods and exercise for obesity management in rats. Male albino rats diagnosed as obese via histopathology and biochemical assays were administered functional foods in common use for obesity management including pomegranate, grapefruit, and red cabbage juice extracts in parallel with swimming exercise. Urine samples were collected from these rats, and likewise from healthy control animals, for metabolite analysis using (GC-MS) coupled to multivariate data analysis. The results revealed a significant elevation in oxalate and phosphate levels in obese rat urine concurrent with lower lactate levels as compared to the control group. Furthermore, and to pinpoint the bioactive agents in the administered functional foods, ultra performance liquid chromatography (UPLC) coupled to high resolution time-of-flight mass spectrometry (TOF-MS) was employed for secondary metabolite profiling. The different phenolic classes found in the examined functional foods, viz. ellagitannins in pomegranate, flavanones in grapefruit and flavonols in red cabbage, are likely to mediate their anti-obesity effects. The results indicate that these functional foods and exercise were quite effective in reverting obesity-related metabolic disruptions back to normal status, as revealed by orthogonal partial least squares-discriminant analysis (OPLS-DA).