RESUMEN
Collagen-based skin-like scaffolds (CBSS) are promising alternatives to skin grafts to repair wounds and injuries. In this work, we propose that the common marine invertebrate sea urchin represents a promising and eco-friendly source of native collagen to develop innovative CBSS for skin injury treatment. Sea urchin food waste after gonad removal was here used to extract fibrillar glycosaminoglycan (GAG)-rich collagen to produce bilayer (2D + 3D) CBSS. Microstructure, mechanical stability, permeability to water and proteins, ability to exclude bacteria and act as scaffolding for fibroblasts were evaluated. Our data show that the thin and dense 2D collagen membrane strongly reduces water evaporation (less than 5% of water passes through the membrane after 7 days) and protein diffusion (less than 2% of BSA passes after 7 days), and acts as a barrier against bacterial infiltration (more than 99% of the different tested bacterial species is retained by the 2D collagen membrane up to 48 h), thus functionally mimicking the epidermal layer. The thick sponge-like 3D collagen scaffold, structurally and functionally resembling the dermal layer, is mechanically stable in wet conditions, biocompatible in vitro (seeded fibroblasts are viable and proliferate), and efficiently acts as a scaffold for fibroblast infiltration. Thus, thanks to their chemical and biological properties, CBSS derived from sea urchins might represent a promising, eco-friendly, and economically sustainable biomaterial for tissue regenerative medicine.
Asunto(s)
Colágenos Fibrilares/farmacología , Fibroblastos/fisiología , Medicina Regenerativa , Erizos de Mar/química , Alimentos Marinos , Piel Artificial , Andamios del Tejido , Residuos , Animales , Técnicas de Cultivo de Célula , Línea Celular , Proliferación Celular , Supervivencia Celular , Cricetinae , Colágenos Fibrilares/química , Colágenos Fibrilares/aislamiento & purificación , Fibroblastos/metabolismo , Manipulación de AlimentosRESUMEN
The importance of fibrillar collagen topology and organization in disease progression and prognostication in different types of cancer has been characterized extensively in many research studies. These explorations have either used specialized imaging approaches, such as specific stains (e.g., picrosirius red), or advanced and costly imaging modalities (e.g., second harmonic generation imaging (SHG)) that are not currently in the clinical workflow. To facilitate the analysis of stromal biomarkers in clinical workflows, it would be ideal to have technical approaches that can characterize fibrillar collagen on standard H&E stained slides produced during routine diagnostic work. Here, we present a machine learning-based stromal collagen image synthesis algorithm that can be incorporated into existing H&E-based histopathology workflow. Specifically, this solution applies a convolutional neural network (CNN) directly onto clinically standard H&E bright field images to extract information about collagen fiber arrangement and alignment, without requiring additional specialized imaging stains, systems or equipment.
Asunto(s)
Biomarcadores de Tumor/aislamiento & purificación , Colágenos Fibrilares/ultraestructura , Imagen Molecular/métodos , Neoplasias/diagnóstico por imagen , Compuestos Azo/química , Biomarcadores de Tumor/química , Progresión de la Enfermedad , Colágenos Fibrilares/aislamiento & purificación , Humanos , Neoplasias/diagnóstico , Neoplasias/patología , Redes Neurales de la Computación , Pronóstico , Microscopía de Generación del Segundo Armónico/métodos , Células del Estroma/ultraestructuraRESUMEN
Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration. The scaffold aids cell organization in the damaged tissue. It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site. In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material. We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair. Animals' well-being was measured by their weight and daily activity post scaffold transplantation during their recovery. At the end of the experiment, the bio-composite was histologically examined, and the surrounding tissues around the implant were evaluated for inflammation reaction and scarring tissue. In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted. At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation. This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair. Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
Asunto(s)
Antozoos/química , Materiales Biocompatibles , Colágenos Fibrilares/química , Implantes Experimentales , Procedimientos Ortopédicos/instrumentación , Lesiones del Manguito de los Rotadores/cirugía , Manguito de los Rotadores/cirugía , Andamios del Tejido , Alginatos/química , Animales , Materiales Biocompatibles/toxicidad , Modelos Animales de Enfermedad , Colágenos Fibrilares/aislamiento & purificación , Colágenos Fibrilares/toxicidad , Reacción a Cuerpo Extraño/etiología , Reacción a Cuerpo Extraño/patología , Hidrogeles , Implantes Experimentales/efectos adversos , Masculino , Procedimientos Ortopédicos/efectos adversos , Diseño de Prótesis , Ratas Wistar , Manguito de los Rotadores/patología , Lesiones del Manguito de los Rotadores/patología , Factores de Tiempo , Andamios del Tejido/efectos adversos , Cicatrización de HeridasRESUMEN
This study presents an innovative multifunctional system in fabricating new functional wound dressing (FWD) products that could be used for skin regeneration, especially in cases of infected chronic wounds and ulcers. The innovation is based on the extraction, characterization, and application of collagen (CO)/chitosan-glucan complex hollow fibers (CSGC)/aloe vera (AV) as a novel FWS. For the first time, specific hollow fibers were extracted with controlled inner (500-900 nm)/outer (2-3 µm) diameters from mycelium of Schizophyllum commune. Further on, research and evaluation of morphology, hydrolytic stability, and swelling characteristics of CO/CSGC@AV were carried out. The obtained FWS showed high hydrolytic stability with enhanced swelling characteristics compared to native collagen. The hemostatic effect of FWS increased significantly in the presence of CSGC, compared to native CO and displayed excellent biocompatibility which was tested by using normal human dermal fibroblast (NHDF). The FWS showed high antibacterial activity against different types of bacteria (positive/negative grams). From in vivo measurements, the novel FWS increased the percentage of wound closure after one week of treatment. All these results imply that the new CO/CSGC@AV-FWD has the potential for clinical skin regeneration and applying for controlled drug release.
Asunto(s)
Aloe , Antibacterianos/farmacología , Vendajes , Quitosano/farmacología , Colágenos Fibrilares/farmacología , Glucanos/farmacología , Micelio , Extractos Vegetales/farmacología , Schizophyllum , Piel/efectos de los fármacos , Cicatrización de Heridas/efectos de los fármacos , Heridas y Lesiones/tratamiento farmacológico , Aloe/química , Animales , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Bacterias/efectos de los fármacos , Bacterias/crecimiento & desarrollo , Células Cultivadas , Quitosano/química , Quitosano/aislamiento & purificación , Modelos Animales de Enfermedad , Colágenos Fibrilares/química , Colágenos Fibrilares/aislamiento & purificación , Glucanos/química , Glucanos/aislamiento & purificación , Humanos , Masculino , Micelio/química , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Ratas Wistar , Schizophyllum/química , Piel/lesiones , Piel/patología , Heridas y Lesiones/patologíaRESUMEN
Here we have developed and validated an original LC-MS/MS SRM procedure flexible enough to quantitatively screen collagen types I-V in copies of the same type of stromal matrix prepared with different protocols of cell removal to retain the native 3D architecture of the ECM. In a first step, identification of tryptic sequences exclusive to specific chains (either α1 or α2) of mammalian collagen standards types I-V was pursued using a combination of LC-LIT-Orbitrap XL and LC-MS/MS SRM analyses. In a second step, the adult male rat thyroid was decellularized using three different protocols specifically set for engineering of bioartificial 3D thyroid organoids. In a third step, DNA analysis of the decellularized 3D thyroid stroma was pursued to exclude contamination by cell nuclear debris. In a final step, collagen standards and 3D thyroid matrices were digested using the same mechanical / enzymatic protocol, and quantitative profiles of collagen types I-V ensued using comparisons of ionic intensities between tryptic peptides of collagen standards and matrices, as derived from targeted LC-MS/MS SRM analysis. Collectively, the procedure allowed for detection and quantitation of collagen types I-V at a femtomolar level in thyroid gland stromal matrices initially maintaining their original 3D architecture, tryptically digested through a method common to collagen standards and thyroid ECM, with satisfactory reproducibility of results, moderate procedural cost, and limited analytical time.
Asunto(s)
Cromatografía Liquida/métodos , Matriz Extracelular/química , Colágenos Fibrilares/análisis , Péptidos/análisis , Espectrometría de Masas en Tándem/métodos , Glándula Tiroides/química , Animales , Colágenos Fibrilares/química , Colágenos Fibrilares/aislamiento & purificación , Límite de Detección , Masculino , Péptidos/aislamiento & purificación , Ratas Sprague-Dawley , Reproducibilidad de los ResultadosRESUMEN
Collagen and collagen fibers have been widely documented as a potential and competitive biomaterial for medical applications. However, the searches for safe and realistic new collagen sources are still underway. Currently, fishery by-products (scales), a promising collagen source are usually discarded. In the present study, in vitro fibril-forming ability of the extracted fish scale collagen is reported. The aim of the investigation was to evaluate the concomitant comparison of fibril-forming abilities and characteristics of acid and pepsin soluble collagens from the scales of Indian major carp catla (Catla catla) and rohu (Labeo rohita). The extracted collagens were characterized as type I, with a total yield of 2.80-4.11% (w/w). Denaturation temperature determined for all collagens were between 35.9 and 37.7°C. All collagens exhibited high solubility in acidic pH and low NaCl concentrations. SEM clarified the lyophilized collagens and their fibril-forming capacity. Amino acid content and radical scavenging efficacy were also analyzed for the extracted collagen. The results revealed that extracted scale collagen from a renewable biological source could be used as biomaterials in various sectors. It might be suitable for preparing collagen gel for biomedical devices or as a scaffold for cell culture because of its high stability and fibril formation capacity.
Asunto(s)
Fenómenos Químicos , Colágenos Fibrilares/química , Temperatura , Aminoácidos/análisis , Estructuras Animales/química , Animales , Antioxidantes/análisis , Rastreo Diferencial de Calorimetría , Carpas , Electroforesis en Gel de Poliacrilamida , Colágenos Fibrilares/aislamiento & purificación , Colágenos Fibrilares/ultraestructura , Pepsina A/metabolismo , Estabilidad Proteica , Solubilidad , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Understanding the viscoelastic behavior of collagenous tissues with complex hierarchical structures requires knowledge of the properties at each structural level. Whole tissues have been studied extensively, but less is known about the mechanical behavior at the submicron, fibrillar level. Using a microelectromechanical systems platform, in vitro coupled creep and stress relaxation tests were performed on collagen fibrils isolated from the sea cucumber dermis. Stress-strain-time data indicate that isolated fibrils exhibit viscoelastic behavior that could be fitted using the Maxwell-Weichert model. The fibrils showed an elastic modulus of 123 ± 46 MPa. The time-dependent behavior was well fit using the two-time-constant Maxwell-Weichert model with a fast time response of 7 ± 2 s and a slow time response of 102 ± 5 s. The fibrillar relaxation time was smaller than literature values for tissue-level relaxation time, suggesting that tissue relaxation is dominated by noncollagenous components (e.g., proteoglycans). Each specimen was tested three times, and the only statistically significant difference found was that the elastic modulus is larger in the first test than in the subsequent two tests, indicating that viscous properties of collagen fibrils are not sensitive to the history of previous tests.
Asunto(s)
Elasticidad , Colágenos Fibrilares/química , Colágenos Fibrilares/aislamiento & purificación , Animales , Sistemas Microelectromecánicos , Modelos Químicos , Pepinos de Mar/química , Estrés Mecánico , Factores de Tiempo , ViscosidadRESUMEN
Although type V collagen (Col V) is present in developing and mature connective tissues of glomeruli, its primary function has not been elucidated yet. The purpose of this study was to elucidate the role of Col V fibrils in glomerular cells. We isolated primary cells from porcine kidney and cultured them on Col V fibrils reconstructed from purified Col V molecules extracted from porcine cornea. Time-lapse observation showed that Col V fibrils induce dynamic movement of glomerular endothelial cells (GEC) by stimulating them to extend long filopodial protrusions and wide lamellipodia. Col V signaling mediated through beta1 integrin activated phosphorylation of paxillin at tyrosine 118 (paxillin-pY118) and of focal adhesion kinase at tyrosine 861 (FAKpY861) at the cell periphery; a second Col V signal was mediated through neuroglycan 2 and activated FAKpY397. FAKpY861 was present in loose attachment points between Col V fibrils and GEC, allowing the cells to migrate easily. Activation of FAKpY397 induced incomplete focal adhesion at the centers of cells and caused cell movement. Therefore both signaling pathways facilitated cell motility, which was inhibited by the addition of antibodies to beta1 integrin, NG2, and Col V. We suggest that Col V fibrils activate 'outside-in' signaling in GEC and induce their dynamic motility.
Asunto(s)
Movimiento Celular , Colágeno Tipo V/fisiología , Células Endoteliales/fisiología , Matriz Extracelular/fisiología , Colágenos Fibrilares/fisiología , Riñón/citología , Animales , Células Cultivadas , Colágeno Tipo V/metabolismo , Colágeno Tipo V/ultraestructura , Matriz Extracelular/metabolismo , Matriz Extracelular/ultraestructura , Colágenos Fibrilares/aislamiento & purificación , Colágenos Fibrilares/metabolismo , Colágenos Fibrilares/ultraestructura , Riñón/metabolismo , Riñón/fisiología , Modelos Biológicos , Fosforilación , Proteínas Quinasas/metabolismo , Receptores de Superficie Celular/fisiología , Transducción de Señal/fisiología , PorcinosRESUMEN
To assess the impact collagen structures may have on glycation, the effects of glucose upon bovine serum albumin, guinea pig skin collagen, rat tail tendon and monomeric collagen were compared under near physiological conditions. Proteins were incubated with or without 50 mM glucose for 64 d in pH 7.4 50 mM phosphate buffer, followed by reduction, acid/alkaline hydrolysis, and analysis. Yields of non-reducible fructose-lysine, in the form of the acid-degradation products furosine and pyridosine, were significantly higher from skin collagen when compared to albumin. Yields of reducible fructose-lysine, in the form of glucitol- and mannitol-lysine, were conversely much greater for albumin, while tail tendon reported intermediate values. Fructose-lysine and unmodified lysine within collagen fibres prior to incubation was therefore protected by the tight packing of the collagen helices, where milling of tail tendon to increase the surface area exposed much of it to reduction protocols. Together with an analysis of pentosidine formation and other products, these results have shown that the interior of the tightly packed skin collagen fibres is protected from both glycation and reduction, and that glycation products differ depending on the protein incubated. Amino acid analysis then showed that our glycated skin collagen was similar to human diabetic skin collagen. Significant quantities of glucose-independent unknowns form in control incubations; their composition again being protein-dependent. The four compound Ks as previously reported were found to be unique to glycated rat tail tendon and soluble collagen, while another glycation product detected in collagen but not albumin may be attributable to carboxymethyl-arginine.
Asunto(s)
Colágenos Fibrilares/metabolismo , Aminoácidos/análisis , Animales , Arginina/análogos & derivados , Arginina/metabolismo , Borohidruros/farmacología , Tampones (Química) , Carbohidratos , Cromatografía Líquida de Alta Presión , Colágenos Fibrilares/química , Colágenos Fibrilares/aislamiento & purificación , Glucosa/metabolismo , Glicosilación , Cobayas , Humanos , Hidrólisis , Lisina/análogos & derivados , Lisina/metabolismo , Espectroscopía de Resonancia Magnética , Oxidación-Reducción/efectos de los fármacos , Ratas , Piel/químicaRESUMEN
This research work allows a direct comparison between collagen solutions of equal concentration derived from the two widely used collagen sources: bovine Achilles tendon (BAT) and rat tail tendon (RTT), and extraction methods: acid (AS) and pepsin (PS) solubilization on the properties of extruded collagen fibers. Scanning electron microscopy revealed that the substructure of the collagen fibers was the same independent of the treatment. Transmission electron microscopy revealed that the AS collagen-derived fibers were comprised of thick quarter-staggered fibrils, while the coexistence of thin nonbanded and thick banded fibrils was apparent for the PS collagen-derived fibers. The BAT-derived fibers demonstrated higher denaturation temperature than the RTT-derived ones (p < 0.05). The extraction method had no influence on the thermal characteristics of the fibers produced (p > 0.05). ASBAT collagen was of higher viscosity than both ASRTT and PSBAT (p < 0.002), and therefore larger diameter fibers were obtained (p < 0.001). An inversely proportional relationship between dry-fiber diameter and stress at break was observed within the treatments. The PS yielded 10 times more soluble collagen from BAT and the derived fibers were of similar tensile strength, stiffness, and elongation (p > 0.05) as those derived from the AS collagen. No significant difference was observed for the stress at break for the ASBAT and the ASRTT, while significant difference was observed for the elongation and modulus values (p < 0.005). Overall, reconstituted collagen fibers were produced with properties similar to native or synthetic fibers to suit a wide range of tissue engineering applications.