RESUMEN
Herbicides pose a potential threat to the soil biodiversity and health. Carfentrazone-ethyl (CE), a triazolinones herbicide, is increasingly used in agricultural production. Its non-target toxic effects on soil microorganisms and soil enzymes are reported recently. However, the sublethal toxicity of CE on soil invertebrates like earthworms is not yet known. Therefore, in this work, the sublethal toxic effects of CE (0.05, 0.5, and 5.0 µg/g in soil) on the soil earthworm (Eisenia fetida) were evaluated using a battery of biomarkers including reactive oxygen species (ROS), enzyme (superoxide dismutase-SOD, catalase-CAT, peroxidase-POD, and glutathione S-transferase-GST) activities, malondialdehyde (MDA) contents, histopathological and DNA damage. Results indicated that CE increased ROS contents, enzyme activities, and MDA contents in the short-time (14 d), thus, causing a slight oxidative stress to E. fetida. However, the toxic effects of CE on earthworms gradually disappeared after 14 days. The CE did not cause histopathological and DNA damage in earthworms. Integrated Biological Response index (IBR) indicated that both concentration and exposure time of CE regulated its sublethal toxicity on earthworms. In conclusion, herbicide CE is safe to soil invertebrate earthworms when applied at the recommended doses. Our results contribute to the current understanding of CE effects on soil earthworms, and can be useful in developing soil health strategies under agrochemical use.
Asunto(s)
Herbicidas/toxicidad , Oligoquetos/efectos de los fármacos , Triazoles/toxicidad , Animales , Ecotoxicología , Enzimas/metabolismo , Herbicidas/administración & dosificación , Intestinos/efectos de los fármacos , Intestinos/patología , Malondialdehído/metabolismo , Oligoquetos/fisiología , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/toxicidad , Triazoles/administración & dosificaciónRESUMEN
Changes in the environment as a result of industrialisation and urbanisation impact negatively on plant growth and crop production. Cadmium (Cd) is one of the most dangerous metals that enters the food chain, with toxic effects on plants and human health. This study evaluated the potential of Silene sendtneri as a novel hyperaccumulator and the role of seed priming in tolerance and accumulation rate of Cd. The effect of different priming agents on germination performance, root growth, seedling development, metal uptake and accumulation, antioxidant defences including enzymatic and non-enzymatic antioxidants has been assessed. Seed priming using silicic acid, proline alone or in combination with salicylic acid- enhanced germination, seedling development, and root growth under Cd stress. The same priming treatments induced an increase of water content in shoots and roots when plants were exposed to Cd. The enzymatic antioxidant response was specific for the priming agent used. An increase in ferulic acid and rutin in shoots was related to the increase of Cd concentration in the medium. The concentration of malic and oxalic acid increased significantly in shoots of plants grown on high Cd concentrations compared to low Cd concentrations. Silene sendtneri can accumulate significant levels of Cd with enhanced accumulation rate and tolerance when seeds are primed. The best results are obtained by seed priming using 1% silicic acid, proline and salicylic acid.
Asunto(s)
Cadmio/administración & dosificación , Prolina/farmacología , Ácido Salicílico/farmacología , Silene/efectos de los fármacos , Ácido Silícico/farmacología , Contaminantes del Suelo/administración & dosificación , Bioacumulación , Tolerancia a Medicamentos , Germinación/efectos de los fármacos , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Semillas/efectos de los fármacos , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Silene/crecimiento & desarrollo , Silene/metabolismoRESUMEN
In 2014, the United States Environmental Protection Agency (EPA) proposed a Dermal Slope Factor (DSF) for benzo[a]pyrene (BaP) of 0.006 (µg/day)-1 (USEPA 2014a). It would make cancer risk estimates associated with soil contact 100 times greater than those from soil ingestion and would predict that a large fraction of skin Basal Cell Carcinomas (BCCs) and Squamous Cell Carcinomas (SCCs) worldwide are caused by low level dermal exposures to PAHs, such as BaP. This is not logical given that sunlight (ultraviolet radiation (UV)) exposure is the generally recognized cause of BCCs and SCCs. This paper critically evaluates the proposed DSF. First, a reality check is performed using EPA standard risk assessment methods and comparing the results to actual BCC and SCC rates in the U.S. population. Then, the biological plausibility of the mechanism by which PAHs might cause human skin cancer is evaluated by exploring the generally recognized etiology of human skin cancer and comparing the genetic mutation signatures of rodent skin tumors caused by PAH exposures to those of human skin cancers. It is concluded that scientific flaws resulted in a proposed DSF value that greatly overestimates the skin cancer risk for humans dermally exposed to BaP in soil.
Asunto(s)
Benzo(a)pireno/toxicidad , Monitoreo del Ambiente/normas , Absorción Cutánea/efectos de los fármacos , Neoplasias Cutáneas/inducido químicamente , Contaminantes del Suelo/toxicidad , Benzo(a)pireno/administración & dosificación , Benzo(a)pireno/metabolismo , Monitoreo del Ambiente/métodos , Humanos , Reproducibilidad de los Resultados , Medición de Riesgo/métodos , Piel/efectos de los fármacos , Piel/metabolismo , Piel/patología , Absorción Cutánea/fisiología , Neoplasias Cutáneas/metabolismo , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/metabolismoRESUMEN
The research objective was to study the presence of DNA damages in haddock exposed to petrogenic or pyrogenic polyaromatic hydrocarbons (PAHs) from different sources: 1) extracts of oil produced water (PW), dominated by 2-ring PAHs; 2) distillation fractions of crude oil (representing oil-based drilling mud), dominated by 3-ring PAHs; 3) heavy pyrogenic PAHs, mixture of 4/5/6-ring PAHs. The biological effect of the different PAH sources was studied by feeding juvenile haddock with low doses of PAHs (0.3-0.7 mg PAH/kg fish/day) for two months, followed by a two-months recovery. In addition to the oral exposure, a group of fish was exposed to 12 single compounds of PAHs (4/5/6-ring) via intraperitoneal injection. The main endpoint was the analysis of hepatic and intestinal DNA adducts. In addition, PAH burden in liver, bile metabolites, gene and protein expression of CYP1A, GST activity, lipid peroxidation, skeletal deformities and histopathology of livers were evaluated. Juvenile haddock responded quickly to both intraperitoneal injection and oral exposure of 4/5/6-ring PAHs. High levels of DNA adducts were detected in livers three days after the dose of the single compound exposure. Fish had also high levels of DNA adducts in liver after being fed with extracts dominated by 2-ring PAHs (a PW exposure scenario) and 3-ring PAHs (simulating an oil exposure scenario). Elevated levels of DNA adducts were observed in the liver of all exposed groups after the 2 months of recovery. High levels of DNA adduct were found also in the intestines of individuals exposed to oil or heavy PAHs, but not in the PW or control groups. This suggests that the intestinal barrier is very important for detoxification of orally exposures of PAHs.
Asunto(s)
Daño del ADN , Gadiformes/crecimiento & desarrollo , Hidrocarburos Policíclicos Aromáticos/toxicidad , Contaminantes del Suelo/toxicidad , Contaminantes Químicos del Agua/toxicidad , Administración Oral , Animales , Hidrocarburo de Aril Hidroxilasas/genética , Gadiformes/genética , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Infusiones Parenterales , Intestinos/química , Hígado/química , Petróleo , Contaminación por Petróleo , Hidrocarburos Policíclicos Aromáticos/administración & dosificación , Contaminantes del Suelo/administración & dosificación , Contaminantes Químicos del Agua/administración & dosificaciónRESUMEN
Response to simultaneous stressors is an important facet of plant ecology and land management. In a greenhouse trial, we studied how eight plant species responded to single and combined effects of three soil concentrations of the phytotoxic munitions constituent RDX and two levels of water-resourcing. In an outdoor trial, we studied the effects of high RDX soil concentration and two levels of water-resourcing in three plant species. Multiple endpoints related to RDX fate, plant health, and plant survival were evaluated in both trials. Starting RDX concentration was the most frequent factor influencing all endpoints. Water-resourcing also had significant impacts, but in fewer cases. For most endpoints, significant interaction effects between RDX concentration and water-resourcing were observed for some species and treatments. Main and interaction effects were typically variable (significant in one treatment, but not in another; associated with increasing endpoint values for one treatment and/or with decreasing endpoint values in another). This complexity has implications for understanding how RDX and water-availability combine to impact plants, as well as for applications like phytoremediation. As an additional product of these greenhouse and outdoor trials, three plants native or naturalized within the southeastern United States were identified as promising species for further study as in situ phytoremediation resources. Plumbago auriculata exhibited relatively strong and markedly consistent among-treatment mean proportional reductions in soil RDX concentrations (112% and 2.5% of the means of corresponding values observed within other species). Likewise, across all treatments, Salvia coccinea exhibited distinctively low variance in mean leaf chlorophyll content index levels (6.5% of the means of corresponding values observed within other species). Both species also exhibited mean wilting and chlorosis levels that were 66% and 35%, and 67% and 84%, of corresponding values observed in all other plants, respectively. Ruellia caroliniensis exhibited at least 43% higher mean survival across all treatments than any other test species in outdoor trials, despite exhibiting similar RDX uptake and bioconcentration levels.
Asunto(s)
Sustancias Explosivas/toxicidad , Plantas/efectos de los fármacos , Contaminantes del Suelo/toxicidad , Triazinas/toxicidad , Acanthaceae/efectos de los fármacos , Acanthaceae/crecimiento & desarrollo , Acanthaceae/fisiología , Biodegradación Ambiental , Sustancias Explosivas/administración & dosificación , Sustancias Explosivas/farmacocinética , Instalaciones Militares , Desarrollo de la Planta/efectos de los fármacos , Fenómenos Fisiológicos de las Plantas/efectos de los fármacos , Plumbaginaceae/efectos de los fármacos , Plumbaginaceae/crecimiento & desarrollo , Plumbaginaceae/fisiología , Salvia/efectos de los fármacos , Salvia/crecimiento & desarrollo , Salvia/fisiología , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/farmacocinética , Sudeste de Estados Unidos , Estrés Fisiológico/efectos de los fármacos , Triazinas/administración & dosificación , Contaminantes Químicos del Agua/administración & dosificación , Contaminantes Químicos del Agua/farmacocinética , Contaminantes Químicos del Agua/toxicidad , Recursos HídricosRESUMEN
Risk assessment conclusions for a site may differ when using site-specific versus default values for the relative bioavailability factor (RBAF) and dermal absorption fraction (ABS.d), because these inputs affect both surface soil screening levels and risk/hazard estimates. Indeed, our case study demonstrates that different conclusions may be reached as to regulatory need for remedial action to protect human health when evaluating soil sampling data for seven carcinogenic polycyclic aromatic hydrocarbons (PAHs) using site-specific versus default TCEQ and USEPA residential soil screening levels. Use of site-specific RBAF and ABS.d values increased carcinogenicity-based TCEQ and USEPA surface soil screening levels for PAHs by 4.4- and 6-fold on average, respectively. Soil screening levels for PAHs were more sensitive to changes in ingestion exposure route parameters than to changes in dermal exposure route parameters. Accordingly, site-specific RBAF and ABS.d information has important implications for screening chemicals at PAH-impacted sites, and in addition provides more realistic estimates of risks/hazards posed by PAHs in soil with reduced uncertainty compared to estimates based on default RBAF and ABS.d values. Although default values are generally deemed acceptable by regulatory agencies, use of risk/hazard estimates based on these default values may compel insufficiently justified remedial action in some instances.
Asunto(s)
Arcilla/química , Hidrocarburos Policíclicos Aromáticos/farmacocinética , Absorción Cutánea/efectos de los fármacos , Contaminantes del Suelo/farmacocinética , Suelo/química , Administración Oral , Disponibilidad Biológica , Humanos , Hidrocarburos Policíclicos Aromáticos/administración & dosificación , Hidrocarburos Policíclicos Aromáticos/efectos adversos , Medición de Riesgo , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/efectos adversos , Propiedades de SuperficieRESUMEN
Sediment microbial communities were exposed for 21 days to an environmental concentration of copper to assess Cu-induced composition changes and resulting effects on microbial sensitivity to acute Cu and As toxicity. Chronic Cu exposure reduced the diversity of the bacterial and archaeal communities from Day 0 to Day 21. The pollution-induced community tolerance concept (PICT) predicts that loss of the most sensitive taxa and gain of more tolerant ones should increase the capacity of Cu-exposed communities to tolerate acute Cu toxicity. Although diversity loss and functional costs of adaptation could have increased their sensitivity to subsequent toxic stress, no increased sensitivity to As was observed. PICT responses varied according to heterotrophic activity, selected as the functional endpoint for toxicity testing, with different results for Cu and As. This suggests that induced tolerance to Cu and As was supported by different species with different metabolic capacities. Ecological risk assessment of contaminants would gain accuracy from further research on the relative contribution of tolerance acquisition and co-tolerance processes on the functional response of microbial communities.
Asunto(s)
Arsénico/toxicidad , Cobre/administración & dosificación , Sedimentos Geológicos/microbiología , Microbiota/efectos de los fármacos , Contaminantes del Suelo/administración & dosificación , Archaea/efectos de los fármacos , Archaea/genética , Bacterias/efectos de los fármacos , Bacterias/genética , Cobre/toxicidad , Tolerancia a Medicamentos , Contaminantes del Suelo/toxicidadRESUMEN
The accumulation of metalloid elements during transfer from contaminated soil to higher trophic levels may potentially result in the exposure of parasitic arthropods to toxic concentrations of these elements. This study examined the transfer of arsenate (As(V)) to aphids (Myzus persicae) from pepper plants cultivated in As(V) contaminated soils of two concentrations (2 and 6â¯mg As(V)/kg dry soil), and the subsequent biological effects on the aphid parasitoid, Aphidius colemani. Results showed that considerable quantities of As(V) were transferred to the plant in a concentration-dependent manner and were partitioned in the plant parts in the order of roots >â¯stems >â¯leaves. The accumulation of As(V) in the aphids increased with the concentrations in the plants; however, the transfer coefficient of As(V) from leaf to aphid was relatively similar and constant (0.07-0.08) at both soil As(V) concentration levels. Increased levels of As(V) significantly affected fecundity and honeydew production in aphids, but survival and developmental time were unaffected. Fecundity (mummification rate) of the parasitoid was not impaired by host As(V) contamination; however, vitality (eclosion rate) was significantly affected. Results are discussed in relation to possible ecological risks posed by the transfer of soil As(V) via the plant-arthropod system to parasitoid arthropods in agroecosystems.
Asunto(s)
Áfidos/metabolismo , Arseniatos/metabolismo , Capsicum/metabolismo , Cadena Alimentaria , Contaminantes del Suelo/metabolismo , Avispas/metabolismo , Animales , Áfidos/crecimiento & desarrollo , Áfidos/parasitología , Arseniatos/administración & dosificación , Relación Dosis-Respuesta a Droga , Ninfa/crecimiento & desarrollo , Ninfa/metabolismo , Ninfa/parasitología , Contaminantes del Suelo/administración & dosificaciónRESUMEN
Ore mining and processing in semi-arid areas is responsible for the generation of metal(loid)-containing dust, which is easily transported by wind to the surrounding environment. To assess the human exposure to dust-derived metal(loid)s (As, Cd, Cu, Pb, Sb, Zn), as well as the potential risks related to incidental dust ingestion, we studied mine tailing dust (nâ¯=â¯8), slag dust (nâ¯=â¯5) and smelter dust (nâ¯=â¯4) from old mining and smelting sites in northern Namibia (Kombat, Berg Aukas, Tsumeb). In vitro bioaccessibility testing using extraction in simulated gastric fluid (SGF) was combined with determination of grain-size distributions, chemical and mineralogical characterizations and leaching tests conducted on original dust samples and separated PM10 fractions. The bulk and bioaccessible concentrations of the metal(loid)s were ranked as follows: mine tailing dustsâ¯<â¯slag dustsâ¯âªâ¯smelter dusts. Extremely high As and Pb bioaccessibilities in the smelter dusts were caused by the presence of highly soluble phases such as arsenolite (As2O3) and various metal-arsenates unstable under the acidic conditions of SGF. The exposure estimates calculated for an adult person of 70â¯kg at a dust ingestion rate of 50â¯mg/day indicated that As, Pb (and also Cd to a lesser extent) grossly exceeded tolerable daily intake limits for these contaminants in the case of slag and smelter dusts. The high risk for smelter dusts has been acknowledged, and the safety measures currently adopted by the smelter operator in Tsumeb are necessary to reduce the staff's exposure to contaminated dust. The exposure risk for the local population is only important at the unfenced disposal sites at Berg Aukas, where the PM10 exhibited high levels of bioaccessible Pb.
Asunto(s)
Polvo , Exposición a Riesgos Ambientales , Metales , Minería , Contaminantes del Suelo , Adulto , Arseniatos , Niño , Polvo/análisis , Monitoreo del Ambiente , Humanos , Metales/administración & dosificación , Metales/análisis , Namibia , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/análisisRESUMEN
The Norwegian Armed Forces' shooting ranges contain contamination by metals such as lead (Pb) and copper (Cu) and are often used as grazing pastures for livestock. To determine whether the sheep were at risk from grazing at a shooting range in Nord-Trøndelag (the Leksdalen shooting field), a study was conducted wherein the aim was to determine the amount of soil the sheep were eating, the accumulation of Cu and Pb in the livers of lambs grazing on the shooting ranges, and the accumulation of Pb and Cu in the grass. The grazing behavior of the sheep was mapped using GPS tracking and wildlife cameras. Soil, grass, feces, and liver samples were collected. All the samples were analyzed for Pb, Cu, and molybdenum (Mo), and soil and feces were also analyzed for titanium (Ti). Mean concentrations in grass, soil, feces, and liver was 41-7189, 1.3-29, 4-5, and 0.3 mg/kg Pb, respectively, and 42-580, 4.2-11.9, 19-23, and 273 mg/kg Cu, respectively. The soil ingestion rate was calculated using Ti in feces and soil. From these results, the theoretical dose of Cu and Pb ingested by grazing sheep was calculated. The soil ingestion rate was found to be 0.1-0.4%, significantly lower than the soil ingestion rate of 5-30% usually used for sheep. Little or no accumulation of Cu and Pb in the grass was found. There was no difference between the metal concentrations in the washed and unwashed grass. According to the calculated dose, the sheep were at little or no risk of acute or chronic Pb and Cu poisoning from grazing on the Leksdalen shooting range. The analysis of liver samples showed that lambs grazing on the shooting range did not have higher levels of Cu or Pb than lambs grazing elsewhere. None of the lambs had concentrations of Cu or Pb in their livers indicating poisoning.
Asunto(s)
Cobre/análisis , Conducta Alimentaria , Plomo/análisis , Poaceae/química , Ovinos , Contaminantes del Suelo/análisis , Suelo/química , Animales , Cobre/administración & dosificación , Dieta , Ingestión de Alimentos , Exposición a Riesgos Ambientales/análisis , Armas de Fuego , Plomo/administración & dosificación , Hígado/metabolismo , Personal Militar , Noruega , Contaminantes del Suelo/administración & dosificación , Titanio/análisisRESUMEN
Metsulfuron-methyl is a common active ingredient recommended for use in pre- and post-emergence control of annual grasses and broadleaf weeds in crops, usually applied with mineral oil as adjuvant to enhance its efficiency. Despite the increasing use of this herbicide, there are no information on its ecotoxicity effects to soil fauna. Avoidance and lethality tests were performed with earthworms and collembolans using tropical artificial soil contaminated with formulated products Ally® (600 g L-1 metsulfuron-methyl) and Assist® (756 g L-1 mineral oil) as adjuvant. Lethality test with earthworms showed no difference when tested with or without adjuvant. When Ally® was tested alone, it caused avoidance behavior only at high concentrations (5000 and 10,000 times field predicted dose). However, Assist® addition changed the response of soil invertebrates increasing the avoidance even at field predicted doses. The toxicity of the adjuvant was confirmed in tests exposing collembolans and earthworms to Assist® alone resulting in avoidance behavior. The results clearly show that the addition of mineral oil enhanced the ecotoxicity of metsulfuron-methyl. This study provides an important contribution to the knowledge on the toxicity of metsulfuron-methyl and indicates that adjuvants should be considered in risk assessment of pesticides, considering that under field conditions, these products are applied together.
Asunto(s)
Artrópodos/efectos de los fármacos , Arilsulfonatos/toxicidad , Ecotoxicología/métodos , Oligoquetos/efectos de los fármacos , Contaminantes del Suelo/toxicidad , Animales , Artrópodos/fisiología , Arilsulfonatos/administración & dosificación , Reacción de Prevención/efectos de los fármacos , Herbicidas/administración & dosificación , Herbicidas/toxicidad , Oligoquetos/fisiología , Contaminantes del Suelo/administración & dosificaciónRESUMEN
Earthworms are important and useful soil organisms, but in agricultural soils, they are potentially exposed to a wide variety of pesticides. Insecticides represent the highest threat to earthworms and many are neurotoxic. There is a need for a reliable, relevant, simple biomarker to assess the sub-lethal effects of neurotoxic insecticides on earthworms under laboratory or field conditions. The Aporrectodea caliginosa earthworms were exposed to 0 (control), 0.5×, 1× (normal field application rate), and 5× concentrations of a carbamate (Pirimor®) and an organophosphate (Lorsban®) insecticides. The nerve conduction velocity (NCV) of the medial giant fibers of A. caliginosa earthworm was recorded on days 0, 1, 2, 3, 4, and 7 to quantify sub-lethal neurotoxic effects. Acetylcholinesterase (AChE) enzyme activity of A. caliginosa homogenates was measured at the conclusion of the experiment. Pirimor® but not Lorsban® induced a significant decrease in NCV on days 3, 4, and 7 at 1× and 5× doses. A significant dose-dependent decrease was observed on AChE activity to Pirimor® at the doses used but not Lorsban®. A clear relationship is observed between AChE activity and NCV in the case of Pirimor®. This study showed that NCV is a sensitive biomarker that correlates well with classical biomarker measurements such as AChE enzyme activity. This technique could be used to study the impact of insecticides on earthworms and also their recovery.
Asunto(s)
Insecticidas/toxicidad , Conducción Nerviosa/efectos de los fármacos , Oligoquetos/efectos de los fármacos , Oligoquetos/fisiología , Contaminantes del Suelo/toxicidad , Acetilcolinesterasa/metabolismo , Agricultura , Animales , Carbamatos/administración & dosificación , Carbamatos/toxicidad , Cloropirifos/administración & dosificación , Cloropirifos/toxicidad , Relación Dosis-Respuesta a Droga , Ecotoxicología/métodos , Biomarcadores Ambientales , Insecticidas/administración & dosificación , Conducción Nerviosa/fisiología , Pirimidinas/administración & dosificación , Pirimidinas/toxicidad , Contaminantes del Suelo/administración & dosificaciónRESUMEN
Di-n-butyl phthalate (DBP) is one of the most ubiquitous plasticizers used worldwide. However, it has negatives effects on the soil, water, atmosphere, and other environmental media and can cause serious pollution. According to the artificial soil test and previous studies, this study was conducted to evaluate the toxicity of earthworms induced by DBP at different concentrations (0, 0.1, 1.0, 10, and 50 mg kg-1) on the 7th, 14th, 21st, and 28th days of exposure. The variations in the antioxidant activities of enzymes, such as catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and glutathione-S-transferase (GST), in the amounts of malondialdehyde (MDA) and reactive oxygen species (ROS) and in the amount of DNA damage were measured to evaluate the toxic impact of DBP in earthworms. Upon exposure to DBP, the SOD, CAT, POD, and GST activities were significantly increased, with the exception of the 0.1 mg kg-1 treatment dose. High concentrations of DBP (10 and 50 mg kg-1) induced superfluous ROS to be produced and caused the MDA content to increase significantly. Therefore, we proposed that DBP led to DNA damage in earthworm coelomocytes in a dose-dependent manner, which means that DBP is a source of oxidative damage and genetic toxicity in earthworms.
Asunto(s)
Daño del ADN/efectos de los fármacos , Dibutil Ftalato/toxicidad , Oligoquetos/efectos de los fármacos , Oligoquetos/genética , Animales , Catalasa/metabolismo , Dibutil Ftalato/administración & dosificación , Relación Dosis-Respuesta a Droga , Ecotoxicología/métodos , Glutatión Transferasa/metabolismo , Malondialdehído/metabolismo , Oligoquetos/metabolismo , Estrés Oxidativo/efectos de los fármacos , Peroxidasa/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/toxicidad , Superóxido Dismutasa/metabolismoRESUMEN
The last generation has witnessed bludgeoning of the world's population, a spike in disease burden, and unprecedented levels of pharmaceutical consumption and production. Unfortunately, pharmaceuticals have left their industrial and household confines and leaked into the environment. Pharmaceuticals are now major environmental pollutants, and are ubiquitous in waters and soils. Unlike other environmental contaminants, pharmaceutical pollutants are not yet regulated globally, simply because acute risk assessments show insignificant human health hazard. But the pitfalls of pharmaceutical pollutants extend beyond acute effects to delayed effects from bioaccumulation, amplified effects from drug-drug interactions, exacerbation of drug resistance, and reduction in aquatic and terrestrial food production. Therefore, ignoring pharmaceutical pollutants deprives society of holistic public health protection.
Asunto(s)
Contaminación Ambiental/análisis , Preparaciones Farmacéuticas/análisis , Salud Pública , Monitoreo del Ambiente/métodos , Contaminación Ambiental/efectos adversos , Humanos , Medición de Riesgo , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/efectos adversos , Contaminantes del Suelo/química , Contaminantes Químicos del Agua/efectos adversos , Contaminantes Químicos del Agua/análisisRESUMEN
Accidental ingestion of contaminated soil has been recognized as an important pathway of human exposure to lead (Pb), especially for children through hand-to-mouth activities. Intake of food following the soil ingestion may affect the bioaccessibility of Pb in the gastrointestinal tract. In this study, the effect of steamed bread on the transformation and subsequent bioaccessibility of Pb in two soils was determined by the physiologically based extraction test (PBET). Two compounds, Pb(NO3)2 and PbCO3, were included in the evaluation for comparison. In the gastric phase, Pb bioaccessibility decreased as the steamed bread increased due to the sorption of Pb on the undissolved steamed bread, especially in PbCO3, Pb bioaccessibility decreased from 95.03% to 85.40%. Whereas in the intestinal phase, Pb bioaccessibility increased from 1.85% to 5.66% and from 0.89% to 1.80% for Pb(NO3)2 and PbCO3, respectively. The increase was attributed to the transformation of formed Pb carbonates into soluble organic-Pb complexes induced by the dissolved steamed bread at neutral pH as indicated by MINTEQ modeling. For the PbCO3-contaminated soil, the change in Pb bioaccessibility in both gastric and intestinal phases behaved like that in the pure PbCO3 compound, the steamed bread increased the bioaccessibility of Pb in the intestinal phase, but the decreased bioaccessibility of Pb was observed in the gastric phase after the steamed bread was added. However, in the soil contaminated with free Pb2+ or sorbed Pb forms, the steamed bread increased the Pb bioaccessibility in both gastric and intestinal phases. This was probably due to the higher dissolved organic carbon induced transformation of sorbed Pb (Pb sorbed by Fe/Mn oxides) into soluble Pb-organic complex. Results from this study indicated that steamed bread had an influence on the Pb speciation transformation, correspondingly affecting Pb bioaccessibility in the gastrointestinal tract.
Asunto(s)
Pan/análisis , Tracto Gastrointestinal/efectos de los fármacos , Plomo/farmacocinética , Contaminantes del Suelo/toxicidad , Disponibilidad Biológica , Biotransformación , Carbonatos/farmacocinética , Fenómenos Químicos , Monitoreo del Ambiente , Tracto Gastrointestinal/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Nitratos/farmacocinética , Suelo/química , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/farmacocinéticaRESUMEN
This article reviews the state of the science regarding oral bioavailability, bioaccessibility, and dermal absorption of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) in soil by humans, and discusses how chemical interactions may control the extent of absorption. Derived from natural and anthropomorphic origins, PAHs occur in a limited number of solid and fluid matrices (i.e., PAH sources) with defined physical characteristics and PAH compositions. Existing studies provide a strong basis for establishing that oral bioavailability of cPAHs from soil is less than from diet, and an assumption of 100% relative bioavailability likely overestimates exposure to cPAHs upon ingestion of PAH-contaminated soil. For both the oral bioavailability and dermal absorption studies, the aggregate data do not provide a broad understanding of how different PAH source materials, PAH concentrations, or soil chemistries influence the absorption of cPAHs from soil. This article summarizes the existing studies, identifies data gaps, and provides recommendations for the direction of future research to support new default or site-specific bioavailability adjustments for use in human health risk assessment.
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Exposición a Riesgos Ambientales/análisis , Hidrocarburos Policíclicos Aromáticos/administración & dosificación , Hidrocarburos Policíclicos Aromáticos/farmacocinética , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/farmacocinética , Administración Oral , Disponibilidad Biológica , Dieta , Ingestión de Alimentos , Humanos , Hidrocarburos Policíclicos Aromáticos/análisis , Medición de Riesgo , Absorción Cutánea , Contaminantes del Suelo/análisisRESUMEN
The aim of the present work was to study the response of a suite of cellular and biochemical markers in the terrestrial snail Cantareus apertus exposed to mercury in view of future use as sensitive tool suitable for mercury polluted soil monitoring and assessment. Besides standardized biomarkers (metallothionein, acetylcholinesterase, and lysosomal membrane stability) novel cellular biomarkers on haemolymph cells were analyzed, including changes in the spread cells/round cells ratio and haemocyte morphometric alterations. The animals were exposed for 14 days to Lactuca sativa soaked for 1h in HgCl2 solutions (0.5 e 1 µM). The temporal dynamics of the responses were assessed by measurements at 3, 7 and 14 days. Following exposure to HgCl2 a significant alteration in the relative frequencies of round cells and spread cells was evident, with a time and dose-dependent increase of the frequencies of round cells with respect to spread cells. These changes were accompanied by cellular morphometric alterations. Concomitantly, a high correspondence between these cellular responses and metallothionein tissutal concentration, lysosomal membrane stability and inhibition of AChE was evident. The study highlights the usefulness of the terrestrial snail C. apertus as bioindicator organism for mercury pollution biomonitoring and, in particular, the use of haemocyte alterations as a suitable biomarker of pollutant effect to be included in a multibiomarker strategy.
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Caracoles Helix/efectos de los fármacos , Hemocitos/efectos de los fármacos , Cloruro de Mercurio/toxicidad , Intoxicación por Mercurio/veterinaria , Contaminantes del Suelo/toxicidad , Acetilcolinesterasa/química , Acetilcolinesterasa/metabolismo , Animales , Biomarcadores/sangre , Forma de la Célula/efectos de los fármacos , Inhibidores de la Colinesterasa/administración & dosificación , Inhibidores de la Colinesterasa/toxicidad , Relación Dosis-Respuesta a Droga , Monitoreo del Ambiente/métodos , Contaminación de Alimentos/prevención & control , Caracoles Helix/enzimología , Caracoles Helix/metabolismo , Hemocitos/patología , Humanos , Membranas Intracelulares/efectos de los fármacos , Membranas Intracelulares/metabolismo , Italia , Lisosomas/efectos de los fármacos , Lisosomas/metabolismo , Cloruro de Mercurio/administración & dosificación , Intoxicación por Mercurio/sangre , Intoxicación por Mercurio/metabolismo , Intoxicación por Mercurio/patología , Metalotioneína/metabolismo , Distribución Aleatoria , Mariscos/análisis , Intoxicación por Mariscos/prevención & control , Contaminantes del Suelo/administración & dosificación , Factores de TiempoRESUMEN
Phytotoxic effect of diesel contaminated soil was investigated on growth and dry matter partitioning in Lactuca sativa and Ipomoea batatas in greenhouse pot experiment at two concentration range (0-30 ml and 0-6 ml diesel kg(-1) soil) for 14 weeks. The results indicated thatwhole plant biomass, stem length, root length, number of leaves and leaf chlorophyll in two plants were negatively correlated with increasing diesel concentrations. The critical concentration of diesel associated with 10% decrease in plant growth was 0.33 ml for lettuce and 1.50 ml for sweet potato. Thus, growth of lettuce in diesel contaminated soil was more sensitive than sweet potato. The pattern of dry matter partitioning between root and shoot in both plants were similar. In 0-6 ml diesel contamination range, allocation of dry matter to shoot system was favoured resulting in high shoot: root ratio of 4.54 and 12.91 for lettuce and sweet potato respectively. However, in 0-30 ml diesel contamination range, allocation of dry matter to root was favoured, which may have been an adaptive mechanism in which the root system was used for storage in addition to increasing the capacity for foraging for mineral nutrients and water. Although lettuce accumulated more metals in its tissue than sweet potato, the tissue mineral nutrients in both species did not vary to great extent. The critical diesel concentration for toxicity suggested that the cause of mortality and poor growth of sweet potato and lettuce grown in diesel contaminated soil was due to presence of hydrocarbons in diesel.
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Gasolina/toxicidad , Ipomoea batatas/toxicidad , Lactuca/efectos de los fármacos , Contaminantes del Suelo/toxicidad , Relación Dosis-Respuesta a Droga , Gasolina/análisis , Ipomoea batatas/química , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/químicaRESUMEN
The rapidly growing literature on the response of edible plants to nanoceria has provided evidence of its uptake and bioaccumulation, which delineates a possible route of entry into the food chain. However, little is known about how the residing organic matter in soil may affect the bioavailability and resulting impacts of nanoceria on plants. Here, we examined the effect of nanoceria exposure (62.5-500 mg/kg) on kidney bean (Phaseolus vulgaris) productivity and seed quality as a function of soil organic matter content. Cerium accumulation in the seeds produced from plants in organic matter enriched soil showed a dose-dependent increase, unlike in low organic matter soil treatments. Seeds obtained upon nanoceria exposure in soils with higher organic matter were more susceptible to changes in nutrient quality. A quantitative proteomic analysis of the seeds produced upon nanoceria exposure provided evidence for upregulation of stress-related proteins at 62.5 and 125 mg/kg nanoceria treatments. Although the plants did not exhibit overt toxicity, the major seed proteins primarily associated with nutrient storage (phaseolin) and carbohydrate metabolism (lectins) were significantly down-regulated in a dose dependent manner upon nanoceria exposure. This study thus suggests that nanoceria exposures may negatively affect the nutritional quality of kidney beans at the cellular and molecular level. More confirmatory studies with nanoceria along different species using alternative and orthogonal "omic" tools are currently under active investigation, which will enable the identification of biomarkers of exposure and susceptibility.
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Cerio/farmacología , Phaseolus/efectos de los fármacos , Semillas/efectos de los fármacos , Metabolismo de los Hidratos de Carbono/efectos de los fármacos , Cerio/administración & dosificación , Cerio/farmacocinética , Cerio/toxicidad , Relación Dosis-Respuesta a Droga , Nanopartículas/administración & dosificación , Nanopartículas/toxicidad , Valor Nutritivo , Phaseolus/metabolismo , Proteínas de Plantas/metabolismo , Proteómica/métodos , Semillas/metabolismo , Contaminantes del Suelo/administración & dosificación , Contaminantes del Suelo/farmacocinética , Contaminantes del Suelo/farmacología , Distribución TisularRESUMEN
The metalloid selenium is an essential element which at slightly elevated concentrations is toxic and mutagenic. In Australia the burning of coal for power generation releases selenium into estuarine environments where it accumulates in sediments. The relationship between selenium exposure, dose and response was investigated in the deposit feeding, benthic, marine bivalve Tellina deltoidalis. Bivalves were exposed in microcosms for 28 days to individual selenium spiked sediments, 0, 5 and 20 µg/g dry mass. T. deltoidalis accumulated selenium from spiked sediment but not in proportion to the sediment selenium concentrations. The majority of recovered subcellular selenium was associated with the nuclei and cellular debris fraction, probably as protein bound selenium associated with plasma and selenium bound directly to cell walls. Selenium exposed organisms had increased biologically detoxified selenium burdens which were associated with both granule and metallothionein like protein fractions, indicating selenium detoxification. Half of the biologically active selenium was associated with the mitochondrial fraction with up to 4 fold increases in selenium in exposed organisms. Selenium exposed T. deltoidalis had significantly reduced GSH:GSSG ratios indicating a build-up of oxidised glutathione. Total antioxidant capacity of selenium exposed T. deltoidalis was significantly reduced which corresponded with increased lipid peroxidation, lysosomal destabilisation and micronuclei frequency. Clear exposure-dose-response relationships have been demonstrated for T. deltoidalis exposed to selenium spiked sediments, supporting its suitability for use in selenium toxicity tests using sub-lethal endpoints.