RESUMEN
Male reproductive health has declined as indicated by increasing rates of cryptorchidism, i.e., undescended testis, poor semen quality, low serum testosterone level, and testicular cancer. Exposure to endocrine disrupting chemicals (EDCs) has been proposed to have a role in this finding. In utero exposure to antiandrogenic EDCs, particularly at a sensitive period of fetal testicular development, the so-called 'masculinization programming window (MPW)', can disturb testicular development and function. Low androgen effect during the MPW can cause both short- and long-term reproductive disorders. A concurrent exposure to EDCs may also affect testicular function or damage testicular cells. Evidence from animal studies supports the role of endocrine disrupting chemicals in development of male reproductive disorders. However, evidence from epidemiological studies is relatively mixed. In this article, we review the current literature that evaluated relationship between prenatal EDC exposures and anogenital distance, cryptorchidism, and congenital penile abnormality called hypospadias. We review also studies on the association between early life and postnatal EDC exposure and semen quality, hypothalamic-pituitary-gonadal axis hormone levels and testicular cancer.
Asunto(s)
Criptorquidismo/patología , Disruptores Endocrinos/efectos adversos , Disgenesia Gonadal/patología , Hipospadias/patología , Reproducción , Neoplasias Testiculares/patología , Criptorquidismo/inducido químicamente , Disgenesia Gonadal/inducido químicamente , Humanos , Hipospadias/inducido químicamente , Masculino , Neoplasias Testiculares/inducido químicamenteRESUMEN
This work describes the relevance of toxicology studies of environmental chemicals, with a focus on phthalates, for a hypothesis that certain human male reproductive disorders and diseases have a common etiology of disturbance of normal development in utero. The "Testicular Dysgenesis Syndrome" hypothesis in humans has parallels in male reproductive tract abnormalities and microscopic lesions reported for phthalate toxicity in rats. Additionally, this work describes the histological findings of abnormal testicular development (testicular dysgenesis) in rats as compared to those in humans, as well as potential findings in rats at different ages, from the embryo to the adult.
Asunto(s)
Disgenesia Gonadal , Ácidos Ftálicos , Enfermedades Testiculares , Animales , Dibutil Ftalato , Disgenesia Gonadal/inducido químicamente , Disgenesia Gonadal/patología , Humanos , Masculino , Ácidos Ftálicos/toxicidad , Ratas , Enfermedades Testiculares/inducido químicamente , Testículo/patologíaRESUMEN
Reproductive health of men has declined over time including reduced semen quality specifically sperm count, increased incidence of infertility, and testicular cancers. Our recent findings suggest that these disease states possibly arise as a result of disruption of testicular stem cells biology by perinatal insults including exposure to endocrine disrupting chemicals. Testicular stem cells include relatively quiescent, very small embryonic-like stem cells (VSELs), and actively dividing spermatogonial stem cells (SSCs). Both VSELs and SSCs express estrogen receptors and are directly vulnerable to endocrine disruption. Exposing mice pups to estradiol (20 µg/pup/day on days 5-7) or diethylstilbestrol (2 µg/pup/day on days 1-5) affected spermatogenesis during adult life with reduced numbers of tubules in stage VIII, tetraploid cells and sperm. These mice were infertile and majority of diethylstilbestrol treated mice revealed testicular cancer-like changes. An increase in VSEL numbers, observed by both flow cytometry and qRT-PCR, was associated with marked reduction of c-KIT positive spermatogonial cells. VSELs undergo epigenetic changes due to endocrine disruption that results in blocked differentiation (impaired spermatogenesis) leading to reduced sperm count and infertility, and their excessive self-renewal initiates cancer-like changes in adult life. Thus, testicular dysgenesis syndrome (TDS) has a stem cell rather than a genetic basis.
Asunto(s)
Disruptores Endocrinos/efectos adversos , Exposición a Riesgos Ambientales/efectos adversos , Disgenesia Gonadal/inducido químicamente , Infertilidad Masculina/etiología , Salud del Hombre , Salud Reproductiva , Células Madre/efectos de los fármacos , Testículo/efectos de los fármacos , Factores de Edad , Animales , Diferenciación Celular/efectos de los fármacos , Disgenesia Gonadal/diagnóstico , Disgenesia Gonadal/metabolismo , Disgenesia Gonadal/fisiopatología , Humanos , Infertilidad Masculina/diagnóstico , Infertilidad Masculina/metabolismo , Infertilidad Masculina/fisiopatología , Masculino , Factores de Riesgo , Espermatogénesis/efectos de los fármacos , Células Madre/metabolismo , Células Madre/patología , Síndrome , Testículo/metabolismo , Testículo/patologíaRESUMEN
Human male reproductive disorders are common and may have a fetal origin - the testicular dysgenesis syndrome (TDS) hypothesis. In rats, experimentally induced TDS disorders result from disruption of fetal androgen production/action specifically in the masculinization programming window (MPW). MPW androgen action also programs longer anogenital distance (AGD) in male versus female rats; shorter male AGD is correlated with risk and severity of induced TDS disorders. AGD thus provides a lifelong, calibrated readout of MPW androgen exposure and predicts likelihood of reproductive dysfunction. Pregnant rat exposure to environmental chemicals, notably certain phthalates (e.g. diethyl hexl phthalate, DEHP; dibutyl phthalate, DBP), pesticides or paracetamol, can reduce fetal testis testosterone and AGD and induce TDS disorders, provided exposure includes the MPW. In humans, AGD is longer in males than females and the presumptive MPW is 8-14 weeks' gestation. Some, but not all, epidemiological studies of maternal DEHP (or pesticides) exposure reported shorter AGD in sons, but this occurred at DEHP exposure levels several thousand-fold lower than are effective in rats. In fetal human testis culture/xenografts, DEHP/DBP do not reduce testosterone production, whereas therapeutic paracetamol exposure does. In humans, androgen production in the MPW is controlled differently (human chorionic gonadotrophin-driven) than in rats (paracrine controlled), and other organs (placenta, liver, adrenals) contribute to MPW androgens, essential for normal masculinization, via the 'backdoor pathway'. Consequently, early placental dysfunction, which is affected by maternal lifestyle and diet, and maternal painkiller use, may be more important than environmental chemical exposures in the origin of TDS in humans.
Asunto(s)
Andrógenos/farmacología , Disgenesia Gonadal/inducido químicamente , Testículo/efectos de los fármacos , Animales , Femenino , Desarrollo Fetal/efectos de los fármacos , Humanos , Masculino , Exposición Materna , Placenta/efectos de los fármacos , Embarazo , RatasRESUMEN
DNA methyltransferases (dnmts) are responsible for DNA methylation and play important roles in organism development. In this study, seven dnmts genes (dnmt1, dnmt2, dnmt3aa, dnmt3ab, dnmt3ba, dnmt3bb.1, dnmt3bb.2) were identified in Nile tilapia. Comprehensive analyses of dnmts were performed using available genome databases from representative animal species. Phylogenetic analysis revealed that the dnmts family were highly conserved in teleosts. Based on transcriptome data from eight adult tilapia tissues, the dnmts were found to be dominantly expressed in the head kidney, testis and ovary. Analyses of the gonadal transcriptome data in different developmental stages revealed that all dnmts were expressed in both ovary and testis, and four de novo dnmts (dnmt3aa, dnmt3ab, dnmt3bb.1, dnmt3bb.2) showed higher expression in the testis than in the ovary. Furthermore, during sex reversal induced by Fadrozole, the expression of these four de novo dnmts increased significantly in treated group compared to female control group. By in situ hybridization, the seven dnmts were found to be expressed mainly in phase I and II oocytes of the ovary and spermatocytes of the testis. When gonads were incubated with a methyltransferase inhibitor (5-AzaCdR) in vitro, the expression of dnmts genes were down-regulated significantly, while the expression of cyp19a1a (a key gene in female pathway) and dmrt1 (a key gene in male pathway) increased significantly. Our results revealed the conservation of dnmts during evolution and indicated a potential role of dnmts in epigenetic regulation of gonadal development.
Asunto(s)
Metilación de ADN , Metilasas de Modificación del ADN/metabolismo , Proteínas de Peces/metabolismo , Regulación del Desarrollo de la Expresión Génica , Ovario/metabolismo , Testículo/metabolismo , Tilapia/fisiología , Secuencia de Aminoácidos , Animales , Secuencia Conservada , Metilación de ADN/efectos de los fármacos , Metilasas de Modificación del ADN/antagonistas & inhibidores , Metilasas de Modificación del ADN/química , Metilasas de Modificación del ADN/genética , Bases de Datos Genéticas , Inhibidores Enzimáticos/farmacología , Epigénesis Genética/efectos de los fármacos , Evolución Molecular , Femenino , Proteínas de Peces/antagonistas & inhibidores , Proteínas de Peces/química , Proteínas de Peces/genética , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Genómica/métodos , Disgenesia Gonadal/inducido químicamente , Disgenesia Gonadal/metabolismo , Disgenesia Gonadal/patología , Isoenzimas/antagonistas & inhibidores , Isoenzimas/química , Isoenzimas/genética , Isoenzimas/metabolismo , Masculino , Especificidad de Órganos , Ovario/citología , Ovario/efectos de los fármacos , Ovario/crecimiento & desarrollo , Filogenia , Testículo/citología , Testículo/efectos de los fármacos , Testículo/crecimiento & desarrollo , Tilapia/genética , Tilapia/crecimiento & desarrollo , Técnicas de Cultivo de Tejidos/veterinariaRESUMEN
Administration of dibutyl phthalate (DBP) to pregnant rats causes reproductive disorders in male offspring, resulting from suppression of intratesticular testosterone, and is used as a model for human testicular dysgenesis syndrome (TDS). DBP exposure in pregnancy induces focal dysgenetic areas in fetal testes that appear between e19.5-e21.5, manifesting as focal aggregation of Leydig cells and ectopic Sertoli cells (SC). Our aim was to identify the origins of the ectopic SC. Time-mated female rats were administered 750 mg/kg/day DBP in three different time windows: full window (FW; e13.5-e20.5), masculinisation programming window (MPW; e15.5-e18.5), late window (LW; e19.5-e20.5). We show that DBP-MPW treatment produces more extensive and severe dysgenetic areas, with more ectopic SC and germ cells (GC) than DBP-FW treatment; DBP-LW induces no dysgenesis. Our findings demonstrate that ectopic SC do not differentiate de novo, but result from rupture of normally formed seminiferous cords beyond e20.5. The more severe testis dysgenesis in DBP-MPW animals may result from the presence of basally migrating GC and a weakened basal lamina, whereas GC migration was minimal in DBP-FW animals. Our findings provide the first evidence for how testicular dysgenesis can result after normal testis differentiation/development and may be relevant to understanding TDS in human patients.
Asunto(s)
Dibutil Ftalato/toxicidad , Disgenesia Gonadal/fisiopatología , Efectos Tardíos de la Exposición Prenatal/fisiopatología , Enfermedades Testiculares/fisiopatología , Animales , Modelos Animales de Enfermedad , Femenino , Feto/efectos de los fármacos , Feto/fisiopatología , Disgenesia Gonadal/inducido químicamente , Humanos , Células Intersticiales del Testículo/efectos de los fármacos , Células Intersticiales del Testículo/patología , Masculino , Embarazo , Efectos Tardíos de la Exposición Prenatal/inducido químicamente , Ratas , Ratas Wistar , Túbulos Seminíferos/efectos de los fármacos , Túbulos Seminíferos/crecimiento & desarrollo , Túbulos Seminíferos/patología , Diferenciación Sexual/efectos de los fármacos , Enfermedades Testiculares/inducido químicamente , Testículo/efectos de los fármacos , Testículo/crecimiento & desarrollo , Testículo/patologíaRESUMEN
The testicular dysgenesis syndrome (TDS) hypothesis, which proposes that common reproductive disorders of newborn and adult human males may have a common fetal origin, is largely untested. We tested this hypothesis using a rat model involving gestational exposure to dibutyl phthalate (DBP), which suppresses testosterone production by the fetal testis. We evaluated if induction of TDS via testosterone suppression is restricted to the "masculinization programming window" (MPW), as indicated by reduction in anogenital distance (AGD). We show that DBP suppresses fetal testosterone equally during and after the MPW, but only DBP exposure in the MPW causes reduced AGD, focal testicular dysgenesis, and TDS disorders (cryptorchidism, hypospadias, reduced adult testis size, and compensated adult Leydig cell failure). Focal testicular dysgenesis, reduced size of adult male reproductive organs, and TDS disorders and their severity were all strongly associated with reduced AGD. We related our findings to human TDS cases by demonstrating similar focal dysgenetic changes in testes of men with preinvasive germ cell neoplasia (GCNIS) and in testes of DBP-MPW animals. If our results are translatable to humans, they suggest that identification of potential causes of human TDS disorders should focus on exposures during a human MPW equivalent, especially if negatively associated with offspring AGD.
Asunto(s)
Disgenesia Gonadal/inducido químicamente , Enfermedades Testiculares/inducido químicamente , Animales , Dibutil Ftalato/toxicidad , Modelos Animales de Enfermedad , Femenino , Humanos , Masculino , Exposición Materna , Plastificantes/toxicidad , RatasRESUMEN
PURPOSE: A wide variety of environmental chemicals/xenobiotics including phthalates have been shown to cause oxidative stress targeting the endocrine system and cause reproductive anomalies. The present review describes various issues by oxidative stress causing male reproductive dysfunctions. Here in this review, the importance and role of phthalate compounds in male reproductive dysfunction has been well documented. MATERIALS AND METHODS: One class of environmental endocrine disruptors is phthalates. Phthalate compounds are mostly used as plasticizers, which increase the flexibility, durability, longevity, and etc. of the plastics. Large-scale use of plastic products in our daily life as well as thousands of workers engaged in the manufacture of plastic and plastic products and recycling plastic industry are potentially exposed to these chemicals. Further, general population as well as vulnerable groups i.e. children and pregnant women are also exposed to these chemicals. Phthalates are among wide variety of environmental toxicants capable of compromising male fertility by inducing a state of oxidative stress in the testes. They may also generate reactive oxygen species (ROS) that may affect various physiological and reproductive functions. RESULTS: The available data points out that phthalate compounds may also induce oxidative stress in the male reproductive organs mainly testis and epididymis. They impair spermatogenic process by inducing oxidative stress and apoptosis in germ cells or target sertoli cells and thereby hamper spermatogenesis. They also impair the Leydig cell function by inducing ROS, thereby decreasing the levels of steroidogenic enzymes. CONCLUSION: Thus in utero and postnatal exposure to phthalate compounds might lead to decreased sperm count and various other reproductive anomalies in the young male.
Asunto(s)
Disruptores Endocrinos/toxicidad , Disgenesia Gonadal/inducido químicamente , Estrés Oxidativo/fisiología , Ácidos Ftálicos/toxicidad , Efectos Tardíos de la Exposición Prenatal/inducido químicamente , Reproducción/efectos de los fármacos , Testículo/anomalías , Daño del ADN/efectos de los fármacos , Disruptores Endocrinos/metabolismo , Exposición a Riesgos Ambientales/efectos adversos , Femenino , Humanos , Infertilidad Masculina/inducido químicamente , Células Intersticiales del Testículo/efectos de los fármacos , Masculino , Estrés Oxidativo/efectos de los fármacos , Ácidos Ftálicos/metabolismo , Embarazo , Recuento de Espermatozoides , Espermatogénesis/efectos de los fármacosRESUMEN
Diisononyl phthalate (DINP) is a synthetic material that has been widely used as a substitute for other plasticizers prohibited due to reproductive toxicity in consumer products. Some phthalates have been associated with testicular dysgenesis syndrome in male fetus when female pregnant dams were exposed to them. The present study investigated effects of DINP on fetal Leydig cell function and testis development. Female pregnant Sprague Dawley rats received control vehicle (corn oil) or DINP (10, 100, 500, and 1000 mg/kg) by oral gavage from gestational day (GD) 12 to 21. At GD 21.5, testicular testosterone production, fetal Leydig cell numbers and distribution, testicular gene and protein expression levels were examined. DINP showed dose-dependent increase of fetal Leydig cell aggregation with the low observed adverse-effect level (LOAEL) of 10 mg/kg and multinucleated gonocyte with LOAEL of 100 mg/kg. At 10 mg/kg, DINP also significantly increased fetal Leydig cell size, but inhibited insulin-like 3 and 3ß-hydroxysteroid dehydrogenase gene expression and protein levels. DINP inhibited testicular testosterone levels at 1000 mg/kg. The results indicate that in utero exposure to DINP affects the expression levels of some fetal Leydig cell steroidogenic genes, gonocyte multinucleation and Leydig cell aggregation.
Asunto(s)
Disgenesia Gonadal/inducido químicamente , Ácidos Ftálicos/toxicidad , Plastificantes/toxicidad , 3-Hidroxiesteroide Deshidrogenasas/metabolismo , Animales , Desmina/metabolismo , Femenino , Expresión Génica/efectos de los fármacos , Disgenesia Gonadal/patología , Células Intersticiales del Testículo/efectos de los fármacos , Masculino , Embarazo , Ratas , Ratas Sprague-Dawley , Testículo/efectos de los fármacos , Testosterona/metabolismoRESUMEN
Realization of program of sex formation in multicellular organisms is a complex multistage process. The role of the inductor in this process is assigned to sex hormones synthesized by cells of the emerging gonads. The action of androgens on the formation of the male is now well understood. However, little is known about the involvement of estrogen the female gonad formation and the formation of a female as a whole. Here we present the results of experimental sex inversion in female chickens produced by aromatase inhibition and by the action of tamoxifen on chicken embryos. We have shown various masculinizing effect depending on the dose of active substance and the number of injections. We have noted that inhibition of aromatase does not block meiotic prophase in oogoniums. We have suggested that there are differences in the mechanisms of action of retinoic acid and estrogens on oogenesis. We have first shown proteins and nucleoproteins that interact with the estrogen receptor 1 and provided maps of their gene localization in human and chicken genomes.
Asunto(s)
Inhibidores de la Aromatasa/farmacología , Antagonistas de Estrógenos/farmacología , Disgenesia Gonadal/genética , Nitrilos/farmacología , Diferenciación Sexual/efectos de los fármacos , Tamoxifeno/farmacología , Triazoles/farmacología , Animales , Embrión de Pollo , Pollos/genética , Mapeo Cromosómico , Receptor alfa de Estrógeno/genética , Receptor alfa de Estrógeno/metabolismo , Femenino , Expresión Génica , Sitios Genéticos , Disgenesia Gonadal/inducido químicamente , Humanos , Letrozol , Masculino , Ovario/efectos de los fármacos , Ovario/metabolismo , Ovario/ultraestructura , Profase , Mapeo de Interacción de Proteínas , Cromosomas Sexuales , Diferenciación Sexual/genética , Testículo/efectos de los fármacos , Testículo/metabolismo , Testículo/ultraestructuraRESUMEN
OBJECTIVE: To investigate the mechanism of di-2-ethylhexyl phthalate (DEHP) and cypermethrin (CYP) inducing gonadal dysgenesis in prepubertal male rats. METHODS: A total of 40 healthy 3-week-old specific pathogen-free male Sprague-Dawley rats were randomly and equally divided into four groups: control group (corn oil), DEHP group (500 mg/kg, dissolved in corn oil), CYP group (80 mg/kg, dissolved in corn oil), and combined exposure group (exposed to 500 mg/kg DEHP and 80 mg/kg CYP, dissolved in corn oil). Rats were treated by gavage administration once a day for 30 days. Twenty-four hours after the last exposure, the animals were sacrificed. The body weight and the wet weight of testis were determined, and the weight coefficient of testis was calculated. Radioimmunoassay was used to determine serum testosterone level. Ultrastructural-level histopathological changes of the testis were examined by transmission electron microscopy. The mRNA and protein expression of follicle stimulating hormone receptor (FSHR), androgen binding protein (ABP), inhibin beta-B (INHBB) and vimentin (VIM) were analyzed by real-time PCR and Western blot, respectively. Factorial design analysis of variance was used to compare differences between groups; interaction diagrams were used to determine the interaction between DEHP and CYP. RESULTS: Compared with those of the control group, the testis weights and testis coefficients of the DEHP, CYP, and combined exposure groups significantly decreased by 39.3-59.2%and 19.7-58.6%, respectively, and all exposure groups showed significant reductions in serum level of testosterone, ranging from 49.1% to 62.7% (P < 0.05 or P < 0.01). And all the exposure groups showed different levels of ultrastructural damages in the testes. Compared with that in the control group, the mRNA expression of FSHR, ABP, INHBB, and VIMin the DEHP group was down-regulated by 1.72, 2.64, 2.83 and 1.79 times, and their protein levels were significantly reduced by 65.2%, 53.7%, 70.1%, and 51.9% (P < 0.05 or P < 0.01). Significant decreases in mRNA expression of ABP (down 1.72 times) and INHBB (down 2.06 times) were observed in the CYP group, and their protein levels decreased by 38.3% and 49.7%, respectively (P < 0.05). The combined exposure to both DEHP and CYP resulted in big decreases in the mRNA levels of FSHR (down 1.62 times), ABP (down 2.00 times), INHBB (down 2.35 times), and VIM (down 1.54 times) and protein levels of FSHR (down 52.1%), INHBB (down 53.9%), and VIM (down 58.8%) (P < 0.05). Factorial design analysis of variance showed that the combination of two substances had an antagonistic effect on the expression of ABP and INHBB (P < 0.05). CONCLUSION: DEHP and CYP, alone or combined, can lead to gonadal dysgenesis in prepubertal male rats. Both of them can disrupt functional mRNA and protein expression in Sertoli cells to certain levels. The combination of DEHP and CYP shows antagonistic effects, and DEHP has a stronger reproductive toxicity than CYP.
Asunto(s)
Dietilhexil Ftalato/toxicidad , Disgenesia Gonadal/inducido químicamente , Piretrinas/toxicidad , Células de Sertoli/metabolismo , Animales , Masculino , Ratas , Ratas Sprague-Dawley , Testículo/citología , Testículo/efectos de los fármacosRESUMEN
CONTEXT: Male reproductive disorders evident at birth or in young adulthood are remarkably common. They are hypothesized to comprise a testicular dysgenesis syndrome (TDS), with a fetal origin involving mild androgen deficiency. EVIDENCE ACQUISITION: Testing this hypothesis requires "seeing back in time." Two ways have been proposed: measurement of anogenital distance (AGD), or measurement of the 2:4 digit length ratio. This review assesses the evidence that they reflect fetal androgen exposure and might be used to provide insight into the origin of TDS disorders. EVIDENCE SYNTHESIS: Supporting evidence for AGD derives from rat experimental studies that identified a fetal masculinization programming window, within which androgen action determines adult reproductive organ size, TDS disorders, and AGD. In humans, AGD is positively correlated to testis size, sperm count/fertility, penis length, and T levels, consistent with rat experimental data. The 2:4 digit ratio also shows associations with these parameters, but inconsistently between studies; evidence that the 2:4 digit ratio accurately reflects fetal androgen exposure is also equivocal. CONCLUSIONS: AGD appears to provide a reliable guide to fetal androgen exposure, although available data are limited. The next steps are to: standardize AGD measurement; obtain age-specific population data; and use AGD to evaluate the importance of fetal androgens in determining reproductive disorders and variation in testis/penis size and sperm count in the normal population. These studies should identify what, if any, clinical applications of AGD measurement are feasible--for example, its ability to predict adult-onset reproductive function and disorders.
Asunto(s)
Canal Anal/anatomía & histología , Andrógenos/efectos adversos , Feto/efectos de los fármacos , Dedos/anatomía & histología , Genitales Masculinos/anatomía & histología , Disgenesia Gonadal/inducido químicamente , Animales , Femenino , Genitales Masculinos/embriología , Humanos , Masculino , Testosterona/sangreRESUMEN
OBJECTIVE: To verify the antagonistic effect of Bushen Tianjing Recipe (BTR) on environmental endocrine disruptors (EEDs) induced gonadal dysgenesis (GD) Sprague-Dawley (SD) male rat model. METHODS: Totally 70 3-week-old male SD rats were randomly divided into seven groups, i.e., the control group (fed with corn oil), the model A group [di-2-ethylhexyl-phthalate (DEHP) 500 mg/kg], the CM A group (fed with DEHP 500 mg/kg + BTR 40 mL/kg), the exposed group B (fed with CYP 80 mg/kg), the CM B group (fed with CYP 80 mg/kg + BTR 40 mL/kg), the model C group [fed with DEHP 500 mg/kg + CYP 80 mL/kg], the CM C group (DEHP 500 mg/kg + CYP 80 mg/kg + BTR 40 mL/kg), respectively, 10 in each group. All were administered with corresponding medication by gastrogavage, once daily, for total 30 days. Rats were killed 24 h after the last administration, and their body weight and wet testis weight were weighed. The coefficient of testis was calculated. The serum testosterone (T) level was measured by chemiluminescent immunoassay. The histopathologic tissue was prepared. The ultrastructural changes of genital cells were observed by electron microscope. RESULTS: Compared with the control group, there was no statistical difference in the body weight increase among all groups (P > 0.05). The time of testicular descent and preputial separation were significantly delayed in each exposed group (P < 0.01). In the exposed group A and the exposed group C, the wet weight of the testes was reduced and serum T level decreased (P < 0.01). The coefficient of testis significantly decreased in the exposed group A (P < 0.01). Compared with corresponding model group, the time of testicular descent and preputial separation were significantly fore-laid in each corresponding CM group (P < 0.01). The weight of the testes, the coefficient of testis, and the serum T level increased in the CM A group (P < 0.01). The serum T level obviously increased in the CM B group (P < 0.05). CONCLUSIONS: The GD rat model was successfully duplicated by using DEHP. EEDs were proved to have significant anti-androgen activities. BTR was verified to have significant antagonistic to its anti-androgen effect.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Disruptores Endocrinos/toxicidad , Disgenesia Gonadal/tratamiento farmacológico , Fitoterapia , Animales , Dietilhexil Ftalato/toxicidad , Disgenesia Gonadal/inducido químicamente , Masculino , Ratas , Ratas Sprague-Dawley , Testosterona/sangreRESUMEN
Phthalate esters are commonly used plasticizers found in many household items, personal care products, and medical devices. Animal studies have shown that in utero exposure to di-(n-butyl) phthalate (DBP) within a critical window during gestation causes male reproductive tract abnormalities resembling testicular dysgenesis syndrome. Our studies utilized p53-deficient mice for their ability to display greater resistance to apoptosis during development. This model was chosen to determine whether multinucleated germ cells (MNG) induced by gestational DBP exposure could survive postnatally and evolve into testicular germ cell cancer. Pregnant dams were exposed to DBP (500 mg/kg/day) by oral gavage from gestational day 12 until birth. Perinatal effects were assessed on gestational day 19 and postnatal days 1, 4, 7, and 10 for the number of MNGs present in control and DBP-treated p53-heterozygous and null animals. As expected, DBP exposure induced MNGs, with greater numbers found in p53-null mice. Additionally, there was a time-dependent decrease in the incidence of MNGs during the early postnatal period. Histologic examination of adult mice exposed in utero to DBP revealed persistence of abnormal germ cells only in DBP-treated p53-null mice, not in p53-heterozygous or wild-type mice. Immunohistochemical staining of perinatal MNGs and adult abnormal germ cells was negative for both octamer-binding protein 3/4 and placental alkaline phosphatase. This unique model identified a role for p53 in the perinatal apoptosis of DBP-induced MNGs and provided insight into the long-term effects of gestational DBP exposure within a p53-null environment.
Asunto(s)
Dibutil Ftalato/toxicidad , Plastificantes/toxicidad , Espermatozoides/efectos de los fármacos , Testículo/efectos de los fármacos , Fosfatasa Alcalina/análisis , Animales , Apoptosis/efectos de los fármacos , Femenino , Proteínas Ligadas a GPI/análisis , Disgenesia Gonadal/inducido químicamente , Disgenesia Gonadal/patología , Isoenzimas/análisis , Masculino , Ratones , Ratones Mutantes , Factor 3 de Transcripción de Unión a Octámeros/análisis , Embarazo , Efectos Tardíos de la Exposición Prenatal , Espermatozoides/patología , Testículo/patología , Proteína p53 Supresora de Tumor/genéticaRESUMEN
The decline in semen quality has been the subject of an animated debate. A recent prospective study now irrefutably shows a decline in semen quality in men from Finland, a country that previously boasted good semen quality. Semen quality has, in some countries, reached a level where a considerable fraction of young men are at risk of fertility problems. Impaired semen quality, testicular cancer, cryptorchidism and hypospadias are risk factors for each other, and the testicular dysgenesis syndrome (TDS) has been put forward to explain the observations. This syndrome implies that the four disease entities share the same patho-physiological etiology caused by disturbed testicular development in early fetal life. It seems likely that the rapid rise in TDS-associated conditions can, at least partly, be explained by environmental factors. Animal studies provide strong evidence that manmade chemicals can disrupt the hormone dependent pathways responsible for fetal gonadal development, subsequently leading to TDS-like symptoms. In humans, fetal exposure to endocrine disrupting substances may play a role, although genetic factors are probably also involved. Recent studies indicate that exposure to endocrine disrupters also in adulthood may affect semen quality and reproductive hormones. Causal relationships are inherently difficult to establish in humans, and a clear connection between the disorders and specific toxicants has not been established. It seems likely that the cumulative effects of various low-dose exposures to endocrine disrupters in our environment are responsible for the adverse effects in the male reproductive system. Semen quality may be the most sensitive marker of adverse environmental exposures, and we suggest that standardized surveillance studies of semen quality are continued or initiated to monitor the combined effects of various preventive actions.
Asunto(s)
Disruptores Endocrinos/toxicidad , Exposición a Riesgos Ambientales , Animales , Biomarcadores , Criptorquidismo/inducido químicamente , Criptorquidismo/epidemiología , Europa (Continente)/epidemiología , Disgenesia Gonadal/inducido químicamente , Disgenesia Gonadal/epidemiología , Disgenesia Gonadal/patología , Humanos , Hipospadias/inducido químicamente , Hipospadias/epidemiología , Infertilidad Masculina/inducido químicamente , Infertilidad Masculina/epidemiología , Masculino , Morbilidad , Salud Reproductiva , Análisis de Semen , Neoplasias Testiculares/inducido químicamente , Neoplasias Testiculares/epidemiologíaRESUMEN
Evidence is accumulating that environmental chemicals (ECs) including endocrine-disrupting compounds (EDCs) can alter female reproductive development, fertility and onset of menopause. While not as clearly defined as in the male, this set of abnormalities may constitute an Ovarian Dysgenesis Syndrome with at least some origins of the syndrome arising during foetal development. ECs/EDCs have been shown to affect trophoblast and placental function, the female hypothalamo-pituitary-gonadal axis, onset of puberty and adult ovarian function. The effects of ECs/EDCs are complex, not least because it is emerging that low-level, 'real-life' mixtures of ECs/EDCs may carry significant biological potency. In addition, there is evidence that ECs/EDCs can alter the epigenome in a sexually dimorphic manner, which may lead to changes in the germ line and perhaps even to transgenerational effects. This review summarises the evidence for EC, including EDC, involvement in female reproductive dysfunction, it highlights potential mechanisms of EC action in the female and emphasises the need for further research into EC effects on female development and reproductive function.
Asunto(s)
Disruptores Endocrinos/toxicidad , Exposición a Riesgos Ambientales , Salud Reproductiva , Animales , Epigénesis Genética/efectos de los fármacos , Femenino , Disgenesia Gonadal/inducido químicamente , Humanos , Sistema Hipotálamo-Hipofisario/efectos de los fármacos , Placenta/efectos de los fármacos , Embarazo , Pubertad/efectos de los fármacos , Factores Sexuales , Trofoblastos/efectos de los fármacosRESUMEN
Cryptorchidism, hypospadias, subfertility and testicular germ-cell tumour have been suggested to comprise a testicular dysgenesis syndrome (TDS) based on the premise that each may derive from perturbations of embryonal programming and gonadal development during foetal life. Endocrine-disrupting chemicals have been hypothesized to be associated with these disorders, given the importance of sex steroid hormones in urogenital development and homeostasis. Organochlorines are one such set of compounds which are defined as containing between one and ten covalently bonded chlorine atoms. These compounds are persistent pollutants with long half-lives, accumulate in adipose tissue when ingested, bioaccumulate and biomagnify, and have complex and variable toxicological profiles. Examples of organochlorines include dichloro-diphenyl-trichloroethane and its metabolites, polychlorinated biphenyls, and chlordane. In this comprehensive review of human epidemiologic studies which have tested for associations between organochlorines and facets of TDS, we find evidence for associations between the exposures p,p'-DDE, cis-nonachlor and trans-nonachlor with testicular germ-cell tumour. The sum of the evidence from human epidemiological studies does not indicate any association between specific organochlorines studied and cryptorchidism, hypospadias or fertility. Many other endocrine-disrupting chemicals, including additional organochlorines, have yet to be assessed in relation to disorders associated with TDS, yet study of such chemicals has strong scientific merit given the relevance of such hypotheses to urogenital development.
Asunto(s)
Disgenesia Gonadal/epidemiología , Hidrocarburos Clorados/toxicidad , Testículo/efectos de los fármacos , Exposición a Riesgos Ambientales , Fertilidad , Disgenesia Gonadal/inducido químicamente , Disgenesia Gonadal/patología , Humanos , Masculino , Testículo/embriología , Testículo/patologíaRESUMEN
There is widespread exposure to anti-androgens, a group of chemicals able to disrupt androgen action in foetal life, with irreversible de-masculinizing consequences. Substances of concern include certain phthalates, pesticides and chemicals used in cosmetics and personal care products. Although people come into contact with several anti-androgens, chemicals risk assessment normally does not take account of the effects of combined exposures. However, a disregard for combination effects may lead to underestimations of risks and for this reason, we have assessed the feasibility of conducting cumulative risk assessment, where the focus is on considering the effects of exposure to multiple chemicals, via multiple routes and pathways. Following recent recommendations by the US National Research Council, we have, for the first time, included phthalates and other anti-androgenic chemicals, a total of 15 substances. On the basis of exposure estimates for the individual chemicals and reference doses for anti-androgenicity, we have used the hazard index approach. We show that the cumulative risks from anti-androgen exposures exceed acceptable levels for people on the upper end of exposure levels. The value obtained for median exposures to the 15 substances can be judged tolerable. However, significant knowledge gaps exist that prevent us from arriving at definitive conclusions. Of greatest concern is an absence of appropriate in vivo toxicity data about large numbers of in vitro androgen receptor antagonists. Knowledge about the effect profiles of these chemicals will lead to higher risk estimates. Our analysis suggests that risk reductions can be achieved by limiting exposures to the plasticizer diethyl hexyl phthalate, the cosmetic ingredients butyl- and propyl paraben, the pesticides vinclozolin, prochloraz and procymidone and bisphenol A.
Asunto(s)
Antagonistas de Andrógenos/toxicidad , Contaminantes Ambientales/toxicidad , Ácidos Ftálicos/toxicidad , Medición de Riesgo/métodos , Animales , Combinación de Medicamentos , Exposición a Riesgos Ambientales , Disgenesia Gonadal/inducido químicamente , Humanos , Masculino , Parabenos/toxicidad , Plaguicidas/toxicidad , Receptores Androgénicos/efectos de los fármacos , Diferenciación Sexual/efectos de los fármacos , Estados UnidosRESUMEN
The aim of this study was to analyse epigenetic (specifically, DNA methylation) change in testes induced by maternal exposure to di-2-(ethylhexyl) phthalate (DEHP) in mice. Testicular dysgenesis syndrome was induced in foetuses by maternal exposure to DEHP. High-performance liquid chromatography was performed to analyse DNA methylation status, and expression levels of the DNA methyltransferases were examined by quantitative real-time polymerase chain reaction and western blotting. DEHP significantly had more than 10% relative increase in the global DNA methylation and also increased DNA methyltransferases' expression. Changes in DNA methylation may play an important role in abnormal testicular function caused by environmental factors such as maternal exposure to DEHP, which may be one possible mechanism of DEHP-mediated testicular toxicity.
Asunto(s)
Metilación de ADN/efectos de los fármacos , Dietilhexil Ftalato/toxicidad , Disgenesia Gonadal/inducido químicamente , Plastificantes/toxicidad , Animales , Western Blotting , Cromatografía Líquida de Alta Presión , Metilasas de Modificación del ADN/genética , Epigénesis Genética/efectos de los fármacos , Femenino , Regulación de la Expresión Génica/efectos de los fármacos , Disgenesia Gonadal/genética , Masculino , Exposición Materna , Ratones , Reacción en Cadena de la Polimerasa , Embarazo , Testículo/efectos de los fármacos , Testículo/patologíaRESUMEN
Pregnancy exposure to di(n-butyl) phthalate (DBP) in rats induces a testicular dysgenesislike syndrome (TDS) in male offspring. Earlier studies suggested altered Sertoli cell development/maturation may result, especially in testes that become cryptorchid. This study quantitatively assessed Sertoli cell numerical and functional development in DBP-exposed rats and compared (unilaterally) cryptorchid and scrotal testes. Pregnant rats were gavaged with 500 mg/kg/day DBP or corn oil from embryonic (E) Days 13.5 to 21.5. Male offspring were sampled on E21.5 or Postnatal Day 6, 10, 15, 25, or 90. Sertoli cell number in DBP-exposed males was reduced by approximately 50% at E21.5 but recovered to normal by Days 25-90, accompanied by significant changes in plasma inhibin B and testosterone levels. Sertoli cell maturational development in DBP-exposed males, assessed using five protein markers (anti-müllerian hormone, cytokeratin, androgen receptor, CDKN1B, and Nestin), was largely normal, with some evidence of delayed maturation. However, in adulthood, Sertoli cells (SC) in areas lacking germ cells (Sertoli cell-only [SCO] tubules) often exhibited immature features, especially in cryptorchid testes. Sertoli cells in DBP-exposed animals supported fewer germ cells during puberty, but this normalized in scrotal testes by adulthood. Scrotal and especially cryptorchid testes from DBP-exposed animals exhibited abnormalities (SCO tubules, focal dysgenetic areas) at all postnatal ages. Cryptorchid testes from DBP-exposed animals exhibited more Sertoli cell abnormalities at Day 25 compared with scrotal testes, perhaps indicating more severe underlying Sertoli cell malfunction in these testes. Our findings support the concept of altered Sertoli cell development in TDS, especially in cryptorchid testes, but show that maturational defects in Sertoli cells in adulthood most commonly reflect secondary dedifferentiation in absence of germ cells.