RESUMEN
Accurate detection of labile analytes through activity based fluorogenic sensing is meaningful but remains a challenge because of nonrapid reaction kinetic. Herein, we present a signaling reporter engineering strategy to accelerate azoreduction reaction by positively charged fluorophore promoted unstable anion recognition for rapidly sensing sodium dithionite (Na2S2O4), a kind of widespread used but harmful inorganic reducing agent. Its quick decomposition often impedes application reliability of traditional fluorogenic probes in real samples because of their slow responses. In this work, four azo-based probes with different charged fluorophores (positive, zwitterionic, neutral, and negative) were synthesized and compared. Among of them, with sequestration effect of positively charged anthocyanin fluorophore for dithionite anion via electrostatic attraction, the cationic probe Azo-Pos displayed ultrafast fluorogenic response (â¼2 s) with the fastest response kinetic (kpos' = 0.373 s-1) that is better than other charged ones (kzwi' = 0.031 s-1, kneu' = 0.013 s-1, kneg' = 0.003 s-1). Azo-Pos was demonstrated to be capable to directly detect labile Na2S2O4 in food samples and visualize the presence of Na2S2O4 in living systems in a timely fashion. This new probe has potential as a robust tool to fluorescently monitor excessive food additives and biological invasion of harmful Na2S2O4. Moreover, our proposed accelerating strategy would be versatile to develop more activity-based sensing probes for quickly detecting other unstable analytes of interest.
Asunto(s)
Colorantes Fluorescentes , Colorantes Fluorescentes/química , Humanos , Ditionita/química , Compuestos Azo/química , CinéticaRESUMEN
Dithionite remained in the foodstuff may pose a great threat to the health of consumers. Three xanthylium-based probes were synthesized and their responses to dithionite were explored. Probe SH-1 could respond to dithionite selectively in PBS buffer (15% DMSO, 10 mM, pH = 7.4). Upon the addition of dithionite, the fluorescent emission of SH-1 at 684 nm dropped quickly (within 10 s) and the fluorescence decline was proportional to the concentration of dithionite (0-7.0 µM). The limit of detection was determined to be 0.139 µM. Then, the sensing mechanism was tentatively presented and the structure of resulted adduct (SH-1-SO3-) which was the reaction product of SH-1 and dithionite via a Micheal addition reaction followed by an oxidation reaction was verified. Moreover, white granulated sugar was subjected to the standard spike experiments and the results demonstrated a great potential of SH-1 for the quantitative monitoring of dithionite in foodstuffs.
Asunto(s)
Ditionita , Colorantes Fluorescentes , Colorantes Fluorescentes/química , Ditionita/química , Espectrometría de Fluorescencia , Contaminación de Alimentos/análisis , Límite de Detección , Azúcares/química , Azúcares/análisisRESUMEN
Whereas dithionite has been extensively used as a reducing agent in soil and sediment remediation, here, we demonstrate that it can be used as a potential source of oxidizing radical in oxic soils with potential application in organic pollutant remediation. Benzoic acid was used as a probe compound and the generation of its oxidative product para-hydroxybenzoic acid (p-HBA) was detected to quantify the production of oxidative radicals (ROS). By increasing the dithionite concentration from 2.5-10 Mm, the accumulated P-HBA concentration in 120 min increased from 15.0-27 µM. Whereas, above 10 mM, the p-HBA concentration decreased due to radical scavenging. Increasing soil dosage from 2.5-15 g/100 mL the accumulated p-HBA amount increased from 22.8-33.7 µM. Temperature 25-35 oC and pH 6.2-7.5 were favoured for p-HBA generation. Furthermore, we investigated the roles of different active intermediates in the reaction system and proposed the mechanism behind the ROS genearation. This study suggested that dithionite can be used as an active reagent for advanced oxidation remediation in oxic soil medium.
Asunto(s)
Estrés Oxidativo , Suelo , Suelo/química , Ditionita/química , Especies Reactivas de Oxígeno , Oxidación-ReducciónRESUMEN
Determining the topology of membrane-inserted proteins and peptides often relies upon indirect fluorescent measurements. One such technique uses NBD, an environmentally sensitive fluorophore that can be covalently linked to proteins. Relative to a hydrophilic environment, NBD in a hydrophobic environment shows an increase in emission intensity and a shift to shorter wavelengths. To gain further insight, NBD fluorescence can be chemically quenched using dithionite. As dithionite is an anion, it is only expected to penetrate the outer leaflet interfacial region and should be excluded from the hydrocarbon core, the inner leaflet, and the lumen of LUV. This assumption holds at neutral pH, where a large number of NBD/dithionite experiments are carried out. Here, we report control experiments in which LUV were directly labeled with NBD-PE to assess dithionite quenching in acidic conditions. Results showed that at acidic pH, dithionite moved more freely across the bilayer to quench the inner leaflet. For the buffer conditions used, dithionite exhibited a sharp change in behavior between pH 5.5 and 6.0. Therefore, in acidic conditions, dithionite could not differentiate in which leaflet the NBD resided.
Asunto(s)
Colorantes Fluorescentes , Proteínas de la Membrana , Ditionita/química , Ditionita/metabolismo , Fluorescencia , Membrana Dobles de Lípidos/química , PéptidosRESUMEN
[FeFe] hydrogenases are highly active enzymes for interconverting protons and electrons with hydrogen (H2). Their active site H-cluster is formed of a canonical [4Fe-4S] cluster ([4Fe-4S]H) covalently attached to a unique [2Fe] subcluster ([2Fe]H), where both sites are redox active. Heterolytic splitting and formation of H2 takes place at [2Fe]H, while [4Fe-4S]H stores electrons. The detailed catalytic mechanism of these enzymes is under intense investigation, with two dominant models existing in the literature. In one model, an alternative form of the active oxidized state Hox, named HoxH, which forms at low pH in the presence of the nonphysiological reductant sodium dithionite (NaDT), is believed to play a crucial role. HoxH was previously suggested to have a protonated [4Fe-4S]H. Here, we show that HoxH forms by simple addition of sodium sulfite (Na2SO3, the dominant oxidation product of NaDT) at low pH. The low pH requirement indicates that sulfur dioxide (SO2) is the species involved. Spectroscopy supports binding at or near [4Fe-4S]H, causing its redox potential to increase by â¼60 mV. This potential shift detunes the redox potentials of the subclusters of the H-cluster, lowering activity, as shown in protein film electrochemistry (PFE). Together, these results indicate that HoxH and its one-electron reduced counterpart Hred'H are artifacts of using a nonphysiological reductant, and not crucial catalytic intermediates. We propose renaming these states as the "dithionite (DT) inhibited" states Hox-DTi and Hred-DTi. The broader potential implications of using a nonphysiological reductant in spectroscopic and mechanistic studies of enzymes are highlighted.
Asunto(s)
Biocatálisis , Ditionita/química , Hidrogenasas/química , Proteínas Hierro-Azufre/química , Sustancias Reductoras/química , Proteínas Algáceas/química , Proteínas Bacterianas/química , Chlamydomonas reinhardtii/enzimología , Clostridium/enzimología , Desulfovibrio desulfuricans/enzimología , Hidrógeno/química , Oxidación-Reducción , Sulfitos/química , Dióxido de Azufre/químicaRESUMEN
A facile and convergent procedure for the synthesis of azobenzene-based probe was reported, which could selectively release interested proteins conducted with sodium dithionite. Besides, the cleavage efficiency is closely associated with the structural features, in which an ortho-hydroxyl substituent is necessary for reactivity. In addition, the azobenzene tag applied in the Ac4GlcNAz-labled proteins demonstrated high efficiency and selectivity in comparison with Biotin-PEG4-Alkyne, which provides a useful platform for enrichment of any desired bioorthogonal proteomics.
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Acetilglucosamina/metabolismo , Alquinos/metabolismo , Azidas/metabolismo , Ditionita/metabolismo , N-Acetilglucosaminiltransferasas/metabolismo , Acetilglucosamina/química , Alquinos/química , Azidas/química , Reacción de Cicloadición , Ditionita/síntesis química , Ditionita/química , Estructura Molecular , N-Acetilglucosaminiltransferasas/química , ProteómicaRESUMEN
Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a new fluorescence microscopy-based assay for detecting phospholipid scramblase activity of membrane proteins upon their reconstitution into giant unilamellar vesicles formed from proteoliposomes by electroformation. The assay is based on chemical bleaching of fluorescence of a photostable ATTO-dye labeled phospholipid with the membrane-impermeant reductant sodium dithionite. We demonstrate that this new methodology is suitable for the study of the scramblase activity of the yeast endoplasmic reticulum at single vesicle level.
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Retículo Endoplásmico/metabolismo , Lípidos/química , Liposomas/química , Proteínas de Transferencia de Fosfolípidos/metabolismo , Levaduras/metabolismo , Transporte Biológico , Biofisica , Membrana Celular/metabolismo , Detergentes/química , Ditionita/química , Fluoresceínas/farmacología , Colorantes Fluorescentes/farmacología , Membrana Dobles de Lípidos/metabolismo , Microscopía Confocal , Microscopía Fluorescente , Fosfolípidos/metabolismo , Liposomas Unilamelares/metabolismoRESUMEN
This study focuses on assessing the possible impact of changes in hemoglobin (Hb) oxygenation on the state of water in its hydration shell as it contributes to red blood cell deformability. Microwave Dielectric Spectroscopy (MDS) was used to monitor the changes in interactions between water molecules and Hb, the number of water molecules in the protein hydration shell, and the dynamics of pre-protein water in response to the transition of Hb from the tense (T) to the relaxed (R) state, and vice versa. Measurements were performed for Hb solutions of different concentrations (5 g/dl-30 g/dl) in phosphate-buffered saline buffer. Cole-Cole parameters of the main water relaxation peak in terms of interactions of water molecules (dipole-dipole/ionic dipole) during the oxygenation-deoxygenation cycle were used to analyze the obtained data. The water mobility-represented by α as a function of ln τ-differed dramatically between the R (oxygenated) state and the T (deoxygenated) state of Hb at physiologically relevant concentrations (30 g/dl-35 g/dl or 4.5 mM-5.5 mM). At these concentrations, oxygenated hemoglobin was characterized by substantially lower mobility of water in the hydration shell, measured as an increase in relaxation time, compared to deoxyhemoglobin. This change indicated an increase in red blood cell cytosolic viscosity when cells were oxygenated and a decrease in viscosity upon deoxygenation. Information provided by MDS on the intraerythrocytic water state of intact red blood cells reflects its interaction with all of the cytosolic components, making these measurements powerful predictors of the changes in the rheological properties of red blood cells, regardless of the cause.
Asunto(s)
Oxihemoglobinas/química , Agua/química , Espectroscopía Dieléctrica , Ditionita/química , Humanos , Microondas , Oxidación-Reducción , Conformación Proteica , ViscosidadRESUMEN
Recent studies have identified the 8C alkyl chain methylimidazolium ionic liquid 1-octyl-3-methylimidazolium in the environment and its potential to trigger the auto-immune liver disease primary biliary cholangitis. The toxicity of a range of methylimidazolium ionic liquids were therefore examined. Oxygen consumption was rapidly inhibited, with potency increasing with alkyl chain length. This preceded caspase 3/7 induction and DNA fragmentation. Time- and dose-dependent loss of dye reduction capacities reflected these effects, with a >700 fold difference in potency between 2C and 10C alkyl chain liquids. None of the ionic liquids directly inhibited mitochondrial complexes I-IV or complex V (F0F1-ATPase). However, dithionite reduction and ESR spectroscopy studies indicate a one electron reduction of oxygen in the presence of a methylimidazolium ionic liquid, suggesting methylimidazolium ionic liquids function as mitochondrial electron acceptors. However, only longer chain ionic liquids form a non-aqueous phase or micelle under aqueous physiological conditions and lead to increases in reactive oxygen species in intact cells. These data therefore suggest that the longer chain methylimidazolium liquids are toxic in sensitive liver progenitor cells because they both readily integrate within the inner mitochondrial membrane and accept electrons from the electron chain, leading to oxidative stress.
Asunto(s)
Transporte de Electrón/efectos de los fármacos , Imidazoles/toxicidad , Líquidos Iónicos/toxicidad , Mitocondrias/efectos de los fármacos , Animales , Bovinos , Línea Celular , Ditionita/química , Glucosa/metabolismo , Hepatocitos/efectos de los fármacos , Humanos , Imidazoles/química , Líquidos Iónicos/química , Ácido Láctico/metabolismo , Estructura Molecular , Oxidación-Reducción , Fosforilación Oxidativa/efectos de los fármacos , Ratas , SmegmamorphaRESUMEN
Polycyclic aromatic hydrocarbons (PAHs), such as naphthalene, are potential health risks due to their carcinogenic and mutagenic effects. Bacteria from the genus Rhodococcus are able to metabolise a wide variety of pollutants such as alkanes, aromatic compounds and halogenated hydrocarbons. A naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038 has been characterised for the first time, using electron paramagnetic resonance (EPR) spectroscopy and UV-Vis spectrophotometry. In the native state, the EPR spectrum of naphthalene 1,2-dioxygenase (NDO) is formed of the mononuclear high spin Fe(III) state contribution and the oxidised Rieske cluster is not visible as EPR-silent. In the presence of the reducing agent dithionite a signal derived from the reduction of the [2Fe-2S] unit is visible. The oxidation of the reduced NDO in the presence of O2-saturated naphthalene increased the intensity of the mononuclear contribution. A study of the "peroxide shunt", an alternative mechanism for the oxidation of substrate in the presence of H2O2, showed catalysis via the oxidation of mononuclear centre while the Rieske-type cluster is not involved in the process. Therefore, the ability of these enzymes to degrade recalcitrant aromatic compounds makes them suitable for bioremediative applications and synthetic purposes.
Asunto(s)
Biodegradación Ambiental , Dioxigenasas/metabolismo , Contaminantes Ambientales/metabolismo , Complejos Multienzimáticos/metabolismo , Naftalenos/metabolismo , Rhodococcus/enzimología , Rhodococcus/metabolismo , Ditionita/química , Espectroscopía de Resonancia por Spin del Electrón , Peróxido de Hidrógeno/química , Oxidación-ReducciónRESUMEN
In this short note we describe the conversion of the widely used fluorescence quenching azo-dyes DABCYL and HABA to fluorophores. The dyes were conjugated to the proteins RNase and human serum albumin (HSA) and subsequently reduced using sodium dithionite (Na2S2O4), thus forming amine-containing fluorophores. Since this chemical reaction can be applied to any azo-containing quencher compound, a great variety of substances can be readily obtained synthetically. This approach provides a promising tool in the use of fluorescence-based investigations of biomolecular interactions.
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Compuestos Azo/química , Colorantes Fluorescentes/química , p-Dimetilaminoazobenceno/análogos & derivados , Secuencia de Aminoácidos , Sitios de Unión , Ditionita/química , Transferencia Resonante de Energía de Fluorescencia , Humanos , Estructura Molecular , Oxidación-Reducción , Unión Proteica , Ribonucleasas/química , Albúmina Sérica/química , Relación Estructura-Actividad , p-Dimetilaminoazobenceno/químicaRESUMEN
Jaggery is a non-centrifugal sweetener produced by thermo-chemical treatments of sugarcane juice. The traditional practices involved in making jaggery are usually tailored using chemicals to meet consumer requirements. Sodium hydrosulphite (hydros) is a commonly employed chemical in the jaggery industry to improve its colour. This article presents a comparative study of jaggery made with and without hydros treatments. The differences in properties, such as sorption behaviour, colour, polyphenols, flavonoids, minerals, and sulphur dioxide content, were measured. Hydros-treated jaggery was found to be brighter in colour with a lower browning index by 5-10. SO2 content of hydros-treated jaggery was >70â¯ppm, while minerals, polyphenols, and flavonoids were less abundant compared to control jaggery, thereby compromising overall quality. Based on the experiments carried out, the optimum treatment of hydros can be employed to satisfy consumer demand while producing an acceptable quality of jaggery that conforms to norms.
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Ditionita/química , Calidad de los Alimentos , Extractos Vegetales/química , Edulcorantes/química , Color , TemperaturaRESUMEN
This study investigates the removal of arsenite (As(III)) from water using dithionite activated by UV light. This work evaluated the removal kinetics of As(III) under UV light irradiation as affected by dithionite dose and light intensity, and characterized the nature of the precipitated solids using XPS and SEM-EDS. Photolysis of dithionite was observed by measuring dithionite concentration using UV absorbance at 315nm. This study also investigated the effect of UV light path length on soluble As concentrations to understand resolubilization mechanisms. Total soluble As concentrations were observed to decrease with reaction time due to reduction of arsenite to form solids having a yellow-orange color. The removal mechanism was found to be reductive precipitation that formed solids of elemental arsenic or arsenic sulfide. However, these solids were observed to resolubilize at later times after dithionite had been consumed. Resolubilization of As was prevented and additional As removal was obtained by frequent dosing of dithionite throughout the experiment. As(III) removal is attributed to photolysis of dithionite by UV light and production of reactive radicals that reduce As(III) and convert it to solid forms.
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Arsenitos/química , Arsenitos/aislamiento & purificación , Precipitación Química , Ditionita/química , Rayos Ultravioleta , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/aislamiento & purificación , Oxidación-Reducción , Fotólisis , SolucionesRESUMEN
Human manganese superoxide dismutase (MnSOD) is one of the most significant enzymes in preventing mitochondrial dysfunction and related diseases by combating reactive oxygen species (ROS) in the mitochondrial matrix. Mitochondria are the source of up to 90% of cellular ROS generation, and MnSOD performs its necessary bioprotective role by converting superoxide into oxygen and hydrogen peroxide. This vital catalytic function is conducted via cyclic redox reactions between the substrate and the active-site manganese using proton-coupled electron transfers. Owing to protons being difficult to detect experimentally, the series of proton transfers that compose the catalytic mechanism of MnSOD are unknown. Here, methods are described to discern the proton-based mechanism using chemical treatments to control the redox state of large perdeuterated MnSOD crystals and subsequent neutron diffraction. These methods could be applicable to other crystal systems in which proton information on the molecule in question in specific chemical states is desired.
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Manganeso/química , Protones , Superóxido Dismutasa/química , Superóxidos/química , Ácido Ascórbico/química , Cristalización , Cristalografía , Deuterio/química , Ditionita/química , Humanos , Peróxido de Hidrógeno/química , Difracción de Neutrones , Oxidación-Reducción , Permanganato de Potasio/química , SolucionesRESUMEN
Magnetic micro-sized mesoporous silica particles were used for the preparation of a gated material able to release an entrapped cargo in the presence of an azo-reducing agent and, to some extent, at acidic pH. The magnetic mesoporous microparticles were loaded with safranin O and the external surface was functionalized with an azo derivative 1 (bearing a carbamate linkage) yielding solid S1. Aqueous suspensions of S1 at pH 7.4 showed negligible safranin O release due to the presence of the bulky azo derivative attached onto the external surface of the inorganic scaffold. However, in the presence of sodium dithionite (azoreductive agent), a remarkable safranin O delivery was observed. At acidic pH, a certain safranin O release from S1 was also found. The pH-triggered safranin O delivery was ascribed to the acid-induced hydrolysis of the carbamate moiety that linked the bulky azo derivatives onto the mesoporous inorganic magnetic support. The controlled release behavior of S1 was also tested using a model that simulated the gastro intestinal tract.
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Compuestos Azo/química , Nanopartículas/química , Dióxido de Silicio/química , Cloruros , Colon/metabolismo , Ditionita/química , Portadores de Fármacos , Liberación de Fármacos , Compuestos Férricos , Compuestos Ferrosos , Humanos , Concentración de Iones de Hidrógeno , Hidrólisis , Magnetismo , Microesferas , Oxidación-Reducción , Fenazinas/administración & dosificación , Porosidad , Propiedades de SuperficieRESUMEN
DNA-encoded chemical libraries have emerged as a cost-effective alternative to high-throughput screening (HTS) for hit identification in drug discovery. A key factor for productive DNA-encoded libraries is the chemical diversity of the small molecule moiety attached to an encoding DNA oligomer. The library structure diversity is often limited to DNA-compatible chemical reactions in aqueous media. Herein, we describe a facile process for reducing aryl nitro groups to aryl amines. The new protocol offers simple operation and circumvents the pyrophoric potential of the conventional method (Raney nickel). The reaction is performed in aqueous solution and does not compromise DNA structural integrity. The utility of this method is demonstrated by the versatile synthesis of benzimidazoles on DNA.
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Bencimidazoles/química , Bencimidazoles/síntesis química , ADN/química , Nitrocompuestos/química , Ditionita/química , Modelos Moleculares , Conformación de Ácido Nucleico , Oxidación-Reducción , Solubilidad , Agua/químicaRESUMEN
A combined process of coagulation-catalytic ozonation-anaerobic sequencing batch reactor (ASBR)-SBR was developed at lab scale for treating a real sodium dithionite wastewater with an initial chemical oxygen demand (COD) of 21,760-22,450â mg/L. Catalytic ozonation with the prepared cerium oxide (CeO2)/granular activated carbon catalyst significantly enhances wastewater biodegradability and reduces wastewater microtoxicity. The results show that, under the optimum conditions, the removal efficiencies of COD and suspended solids are averagely 99.3% and 95.6%, respectively, and the quality of final effluent can meet the national discharge standard of China. The coagulation and ASBR processes remove a considerable proportion of organic matter, while the SBR plays an important role in post-polish of final effluent. The ecotoxicity of the wastewater is greatly reduced after undergoing the hybrid treatment. This work demonstrates that the hybrid system has the potential to be applied for the advanced treatment of high-strength industrial wastewater.
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Ditionita/química , Contaminantes Químicos del Agua/química , Purificación del Agua , Reactores Biológicos , China , Residuos Industriales , Ozono , Eliminación de Residuos Líquidos , Aguas ResidualesRESUMEN
Large amounts of chromite ore processing residue (COPR) wastes have been deposited in many countries worldwide, generating significant contamination issues from the highly mobile and toxic hexavalent chromium species (Cr(VI)). In this study, sodium dithionite (Na2S2O4) was used to reduce Cr(VI) to Cr(III) in COPR containing high available Fe, and then sodium phosphate (Na3PO4) was utilized to further immobilize Cr(III), via a two-step procedure (TSP). Remediation and immobilization processes and mechanisms were systematically investigated using batch experiments, sequential extraction studies, X-ray diffraction (XRD) and X-ray Photoelectron Spectroscopy (XPS). Results showed that Na2S2O4 effectively reduced Cr(VI) to Cr(III), catalyzed by Fe(III). The subsequent addition of Na3PO4 further immobilized Cr(III) by the formation of crystalline CrPO4·6H2O. However, addition of Na3PO4 simultaneously with Na2S2O4 (via a one-step procedure, OSP) impeded Cr(VI) reduction due to the competitive reaction of Na3PO4 and Na2S2O4 with Fe(III). Thus, the remediation efficiency of the TSP was much higher than the corresponding OSP. Using an optimal dosage in the two-step procedure (Na2S2O4 at a dosage of 12× the stoichiometric requirement for 15 days, and then Na3PO4 in a molar ratio (i.e. Na3PO4: initial Cr(VI)) of 4:1 for another 15 days), the total dissolved Cr in the leachate determined via Toxicity Characteristic Leaching Procedure (TCLP Cr) testing of our samples was reduced to 3.8 mg/L (from an initial TCLP Cr of 112.2 mg/L, i.e. at >96% efficiency).
Asunto(s)
Cromo/química , Ditionita/química , Fosfatos/química , Compuestos Férricos/química , Residuos Industriales , Espectroscopía de Fotoelectrones , Difracción de Rayos XRESUMEN
Soil washing is a promising way to remediate arsenic-contaminated soils. Most research has mostly focused on seeking efficient extractants for removing arsenic, but not concerned with any changes in soil properties when using this technique. In this study, the removal of arsenic from a heavily contaminated soil employing different washing solutions including H3PO4, NaOH and dithionite in EDTA was conducted. Subsequently, the changes in soil physicochemical properties and phytotoxicity of each washing technique were evaluated. After washing with 2 M H3PO4, 2 M NaOH or 0.1 M dithionite in 0.1 M EDTA, the soil samples' arsenic content met the clean-up levels stipulated in China's environmental regulations. H3PO4 washing decreased soil pH, Ca, Mg, Al, Fe, and Mn concentrations but increased TN and TP contents. NaOH washing increased soil pH but decreased soil TOC, TN and TP contents. Dithionite in EDTA washing reduced soil TOC, Ca, Mg, Al, Fe, Mn and TP contents. A drastic color change was observed when the soil sample was washed with H3PO4 or 0.1 M dithionite in 0.1 M EDTA. After adjusting the soil pH to neutral, wheat planted in the soil sample washed by NaOH evidenced the best growth of all three treated soil samples. These results will help with selecting the best washing solution when remediating arsenic-contaminated soils in future engineering applications.
Asunto(s)
Arsénico/química , Restauración y Remediación Ambiental/métodos , Contaminantes del Suelo/química , Suelo/química , Arsénico/análisis , China , Ditionita/química , Ácido Edético/química , Ácidos Fosfóricos/química , Hidróxido de Sodio/química , Contaminantes del Suelo/análisisRESUMEN
Sorafenib and regorafenib are small-molecule kinase inhibitors approved for the treatment of locally recurrent or metastatic, progressive, differentiated thyroid carcinoma, renal cell carcinoma, and hepatocellular carcinoma (sorafenib) and of colorectal cancer (regorafenib). As of now, the mechanisms, which are responsible for their antitumor activities, are not completely understood. Given the lipophilic nature of the molecules, it can be hypothesized that the pharmacological impact is mediated by the interaction with cellular membranes as it is true for many pharmacologically active molecules. However, an interaction of sorafenib or regorafenib with lipid membranes has not yet been investigated in detail. Here, we characterized the interaction of both drugs with lipid membranes by applying a variety of biophysical approaches including nuclear magnetic resonance, electron spin resonance, and fluorescence spectroscopy. We found that sorafenib and regorafenib bind to lipid membranes by inserting into the lipid-water interface of the bilayer. This membrane embedding causes a disturbance of bilayer structure leading to an increased permeability of the membrane for polar molecules. One approach shows that the extent of the effects depends on the membrane lipid composition underlining a particular role of phosphatidylcholine and cholesterol. Our data for the first time characterize the impact of sorafenib and regorafenib on the lipid membrane structure and dynamics, which may contribute to a better understanding of their effectiveness in the treatment of malignancies as well as of their side effects.