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INTRODUCTION/AIMS: Very few studies analyzing the pattern of muscle involvement in magnetic resonance imaging (MRI) of patients with McArdle disease have been reported to date. We aimed to examine the pattern of muscle fat replacement in patients with McArdle disease. METHODS: We performed a retrospective study including all patients with genetically confirmed McArdle disease followed in our center from January 2010 to March 2021. Clinical data were collected from the medical record. Whole-body MRI was performed as part of the diagnostic evaluation. The distribution of muscle fat replacement and its severity were analyzed. RESULTS: Nine patients were included. Median age at onset was 7 y (range, 5-58) and median age at the time when MRI was performed was 57.3 y (range, 37.2-72.8). At physical examination, four patients had permanent weakness: in three the weakness was limited to paraspinal muscles, whereas in one the weakness involved the paraspinal and proximal upper limb muscles. Muscle MRI showed abnormalities in six of the seven studied patients. In all of them, fat replacement of paravertebral muscles was found. Other muscles frequently affected were the tongue in three, subscapularis in three, and long head of biceps femoris and semimembranosus in two. DISCUSSION: Our findings suggest that paraspinal muscle involvement is common in McArdle disease and support the need to include this disease in the differential diagnosis of the causes of paraspinal muscle weakness. Involvement of the tongue and subscapularis are also frequent in McArdle disease.
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Enfermedad del Almacenamiento de Glucógeno Tipo V , Músculos Paraespinales , Adulto , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Enfermedad del Almacenamiento de Glucógeno Tipo V/patología , Humanos , Imagen por Resonancia Magnética , Debilidad Muscular/etiología , Músculo Esquelético/diagnóstico por imagen , Músculo Esquelético/patología , Músculos Paraespinales/diagnóstico por imagen , Prevalencia , Estudios RetrospectivosRESUMEN
This study describes muscle involvement on whole-body MRI (WB-MRI) scans at different stages of McArdle disease. WB-MRI was performed on fifteen genetically confirmed McArdle disease patients between ages 25 to 80. The degree of fatty substitution was scored for 60 muscles using Mercuri's classification. All patients reported an intolerance to exercise and episodes of rhabdomyolysis. A mild fixed muscle weakness was observed in 13/15 patients with neck flexor weakness in 7/15 cases, and proximal muscle weakness in 6/15 cases. A moderate scapular winging was observed in five patients. A careful review of the MRI scans, as well as hierarchical clustering of patients by Mercuri scores, pointed out recurrent muscle changes particularly in the subscapularis, anterior serratus, erector spinae and quadratus femoris muscles. WB-MRI imaging provides clinically relevant information and is a useful tool to orient toward the diagnosis of McArdle disease.
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Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Músculo Esquelético/diagnóstico por imagen , Imagen de Cuerpo Entero/métodos , Adulto , Anciano , Anciano de 80 o más Años , Ejercicio Físico , Femenino , Humanos , Masculino , Persona de Mediana Edad , Debilidad Muscular , Rabdomiólisis/diagnóstico por imagen , Muslo/diagnóstico por imagenRESUMEN
Background: We present a longitudinal clinical characterization of PYGM-linked pattern dystrophy in an adult male patient.Materials and Methods: A patient affected by McArdle disease (glycogen storage disease type V) and homozygous for the nonsense variant PYGM c.148C>T p.(Arg50*) underwent ophthalmic examinations over a 9-year-interval, including fundus photography, fundus autofluorescence, optical coherence tomography (OCT), OCT-angiography and electroretinography (ERG).Results: At age 52, the patient was asymptomatic but yellow flecks were first observed in the macula of both eyes. This yellow flecks at the posterior pole progressed towards a pattern-like dystrophy over a 5-year-period. By fundus autofluorescence imaging the appearance of new hyperautofluorescent flecks and the extension of existing ones was observed over time. Concomitantly, a slow progression of the size of atrophic areas was seen at the posterior pole. Scotopic ERGs were within normal limits, but photopic Flicker responses were decreased, indicating reduced cone function.Conclusions: This additional case of PYGM-linked pattern dystrophy further confirms retinopathy as a clinical phenotype associated with McArdle disease. PYGM expression pattern suggests a disease mechanism involving impaired glycogen metabolism both in the retinal pigment epithelium and in cone photoreceptors.
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Electrorretinografía/métodos , Angiografía con Fluoresceína/métodos , Enfermedad del Almacenamiento de Glucógeno Tipo V/patología , Enfermedades de la Retina/patología , Tomografía de Coherencia Óptica/métodos , Enfermedad del Almacenamiento de Glucógeno Tipo V/complicaciones , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Humanos , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Fenotipo , Células Fotorreceptoras Retinianas Conos , Enfermedades de la Retina/complicaciones , Enfermedades de la Retina/diagnóstico por imagen , Agudeza VisualRESUMEN
PURPOSE: To evaluate differences in diffusion parameters in thigh muscles in patients with glycogen storage disease type V (McArdle disease) using muscle diffusion tensor imaging (mDTI) compared to healthy controls METHODS: In this prospective study, we evaluated thigh muscles from hip to knee of 10 McArdle patients (5 female, mean age 33.7 ± 14.4 years) and 10 healthy age- and gender-matched volunteers. MRI scans were performed at 3 T and comprised mDTI, T1-weighted and T2-weighted imaging between May 2015 and May 2017. Needle biopsy of the vastus lateralis muscle was performed in three McArdle patients. The muscle tissue was analyzed by using histochemical and enzyme-histochemical techniques for glycogen content and histopathological changes. Mean values of the eigenvalues (λ1-λ3), fractional anisotropy (FA), and mean diffusivity (MD) were obtained for the vastus lateralis, vastus medialis, rectus femoris, biceps femoris, semitendinosus, and semimembranosus and compared between groups using Student's t tests, as well as ANCOVA; significance level was set at p < 0.05. RESULTS: Needle biopsy showed intracellular glycogen accumulation in skeletal muscle fibers of three McArdle patients. Extracellular histopathological changes were not found. Muscle DTI analysis did not show statistically significant differences between patients and controls for any of the muscles. CONCLUSION: Despite intracellular glycogen accumulation in the three biopsy samples, mDTI parameters were not altered in McArdle patients compared to controls. We conclude that the currently used mDTI acquisition and processing lack the sensitivity to detect intracellular changes due to accumulated glycogen in this cohort of McArdle patients. KEY POINTS: ⢠Despite intracellular glycogen accumulation in three examined biopsy samples, mDTI parameters were not altered in McArdle patients compared to controls. ⢠In its current form, diffusion MR does not provide additional information in quantifying intracellular glycogen accumulations within skeletal muscle fibers in McArdle patients.
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Imagen de Difusión Tensora , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Músculo Esquelético/diagnóstico por imagen , Muslo/diagnóstico por imagen , Adulto , Anisotropía , Femenino , Enfermedad del Almacenamiento de Glucógeno Tipo V/patología , Músculos Isquiosurales/diagnóstico por imagen , Músculos Isquiosurales/patología , Humanos , Masculino , Persona de Mediana Edad , Músculo Esquelético/patología , Estudios Prospectivos , Músculo Cuádriceps/diagnóstico por imagen , Músculo Cuádriceps/patología , Muslo/patología , Adulto JovenRESUMEN
INTRODUCTION: The aim of this study was the label-free identification of distinct myopathological features with coherent anti-Stokes Raman scattering (CARS) imaging, which leaves the sample intact for further analysis. METHODS: The protein distribution was determined without labels by CARS at 2,930 cm-1 and was compared with the results of standard histological staining. RESULTS: CARS imaging allowed the visualization of glycogen accumulation in glycogen storage disease type 5 (McArdle disease) and of internal nuclei in centronuclear myopathy. CARS identified an inhomogeneous protein distribution within muscle fibers in sporadic inclusion body myositis that was not shown with standard staining. In Duchenne muscular dystrophy, evidence for a higher protein content at the border of hypercontracted fibers was detected. DISCUSSION: CARS enables the label-free identification of distinct myopathological features, possibly paving the way for subsequent proteomic, metabolic, and genomic analyses. Muscle Nerve 58: 457-460, 2018.
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Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Enfermedad del Almacenamiento de Glucógeno Tipo V/metabolismo , Microscopía Óptica no Lineal/métodos , Preescolar , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Espectrometría Raman/métodosRESUMEN
McArdle disease is caused by deficiency of myophosphorylase, the muscle isoform of glycogen phosphorylase. This isoform is also expressed in astrocytes, where it seems to have a key role in neural energy metabolism. Whereas in other glycogen storage diseases cognitive impairment has been rarely reported, it has not been previously observed in McArdle disease. Here we report the case of an Italian 55-year-old woman with McArdle disease and cognitive impairment with bilateral dysfunction of prefrontal and frontal cortex (shown by neuropsychological assessment and fluorodeoxyglucose PET). Further studies are needed to assess the prevalence of central neurological manifestations in this disease.
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Trastornos del Conocimiento/complicaciones , Enfermedad del Almacenamiento de Glucógeno Tipo V/complicaciones , Anciano , Encéfalo/diagnóstico por imagen , Encéfalo/patología , Corteza Cerebral/diagnóstico por imagen , Trastornos del Conocimiento/diagnóstico por imagen , Femenino , Fluorodesoxiglucosa F18 , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Humanos , Pruebas Neuropsicológicas , Tomografía de Emisión de Positrones/métodosRESUMEN
In McArdle's disease (myophosphorylase deficiency) exercise intolerance is generally attributed to a lack of glycogenolysis, which decreases energy production during exercise. Magnetic resonance imaging data have recently suggested an impairment of the increase in muscle perfusion during exercise in these patients. We have tested this hypothesis by direct measurement of local muscle perfusion increase. Increase in muscle perfusion was assessed by positron emission tomography with oxygen-15 labelled water in five patients with McArdle's disease and five age- and sex-matched healthy volunteers. Radioactivity was measured in both forearms before and after exercise of the right forearm. The exercise intensity was biochemically assessed by in vivo phosphorus-31 magnetic resonance spectroscopy. The estimated increase in muscle perfusion with exercise was 5.7+/-5.5-fold in the patients (range 1.5-12.8) and 22.3+/-12.0-fold in the healthy subjects (range 10.1-37) (P=0.022). The results show a significant impairment of increase in muscle perfusion with exercise in McArdle's disease. Thus patients may suffer not only from a direct lack of glycogenolysis but also from indirectly impaired vasodilation.
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Enfermedad del Almacenamiento de Glucógeno Tipo V/fisiopatología , Músculo Esquelético/irrigación sanguínea , Esfuerzo Físico , Adulto , Femenino , Antebrazo , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Enfermedad del Almacenamiento de Glucógeno Tipo V/metabolismo , Humanos , Espectroscopía de Resonancia Magnética , Masculino , Persona de Mediana Edad , Músculo Esquelético/diagnóstico por imagen , Músculo Esquelético/metabolismo , Fosfocreatina/metabolismo , Tomografía Computarizada de EmisiónAsunto(s)
Difosfonatos/metabolismo , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Enfermedad del Almacenamiento de Glucógeno/diagnóstico por imagen , Músculos/metabolismo , Fosforilasas/deficiencia , Compuestos de Tecnecio , Tecnecio/metabolismo , Enfermedad del Almacenamiento de Glucógeno Tipo V/metabolismo , Glucólisis , Humanos , Masculino , Persona de Mediana Edad , CintigrafíaRESUMEN
A 57-year-old patient suffering from late-onset McArdle's disease developed myoglobinaemia, massive myoglobinuria and marked serum creatine kinase elevation subsequent to a routinely performed forearm ischaemic work test. Twenty hours after the test, enhancement of 99mTc methylene-diphosphonate activity was demonstrated exclusively in the tested forearm. It is concluded that the forearm ischaemic work test is potentially hazardous to McArdle patients, as it might induce myoglobinuria sufficient to result in acute myoglobinuric renal failure.
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Prueba de Esfuerzo/efectos adversos , Antebrazo/irrigación sanguínea , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico , Enfermedad del Almacenamiento de Glucógeno/diagnóstico , Isquemia/complicaciones , Enfermedad del Almacenamiento de Glucógeno Tipo V/diagnóstico por imagen , Humanos , Isquemia/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Mioglobinuria/diagnóstico por imagen , Mioglobinuria/etiología , CintigrafíaRESUMEN
This paper reports the findings on two patients with McArdle syndrome (myophosphorylase deficiency) in whom conventional bone scans with Tc-99m pyrophosphate revealed intense muscle labeling following exercise tests. The temporal pattern observed was similar to that seen with other types of muscle damage. The prolonged cramps often occurring with this entity appears to produce muscle damage that is readily demonstrable using conventional bone-scanning techniques.