RESUMEN
A large number of experimental studies has demonstrated that angiotensin II (Ang II) is involved in key events of the inflammatory process. This study aimed to evaluate the role of Ang II type 1 (AT1) and Ang II type 2 (AT2) receptors on periodontitis. Methods: Experimental periodontitis was induced by placing a 5.0 nylon thread ligature around the second upper left molar of AT1 mice, no-ligature or ligature (AT1-NL and AT1-L), AT2 (AT2-NL or AT2-L) and wild type (WT-NL or L). Alveolar bone loss was scanned using Micro-CT. Cytokines, peptides and enzymes were analyzed from gingival tissues by Elisa and RT-PCR. Results: The blockade of AT1 receptor resulted in bone loss, even in healthy animals. Ang II receptor blockades did not prevent linear bone loss. Ang II and Ang 1-7 levels were significantly increased in the AT2-L (p < 0.01) group compared to AT2-NL and AT1-L. The genic expression of the Mas receptor was significantly increased in WT-L and AT2-L compared to (WT-NL and AT2-NL, respectively) and in AT1-L. Conclusions: Our data suggest that the receptor AT1 appears to be important for the maintenance of bone mass. AT2 receptor molecular function in periodontitis appears to be regulated by AT1.
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Pérdida de Hueso Alveolar/metabolismo , Enfermedades Mandibulares/metabolismo , Periodontitis/metabolismo , Receptor de Angiotensina Tipo 1/metabolismo , Receptor de Angiotensina Tipo 2/metabolismo , Pérdida de Hueso Alveolar/genética , Pérdida de Hueso Alveolar/patología , Angiotensina II/metabolismo , Animales , Modelos Animales de Enfermedad , Masculino , Enfermedades Mandibulares/genética , Enfermedades Mandibulares/patología , Ratones , Ratones Noqueados , Periodontitis/genética , Periodontitis/patología , Receptor de Angiotensina Tipo 1/genética , Receptor de Angiotensina Tipo 2/genéticaRESUMEN
Previously, severe upper airway obstruction in patients with retrognathia and glossoptosis has been managed with tracheostomy. However, tracheostomy is associated with significant morbidity. In recent years, mandibular distraction has become an alternative management strategy in infants, but these applications have been limited to patient populations with retrognathia and glossoptosis. The authors present 2 unique cases of patients with KAT6B-related gene disorders, who present with a paradox of tongue-based airway obstruction in the absence of retrognathia. In both cases mandibular distraction osteogenesis with an obliquely oriented vector was successfully performed and both children avoided the need for tracheostomy.
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Histona Acetiltransferasas/genética , Enfermedades Mandibulares/genética , Osteogénesis por Distracción , Obstrucción de las Vías Aéreas/cirugía , Glosoptosis , Humanos , Lactante , Recién Nacido , Masculino , Enfermedades Mandibulares/cirugía , Osteogénesis por Distracción/métodos , Retrognatismo/cirugía , TraqueostomíaRESUMEN
OBJECTIVES: The aim of this study was to investigate the role of p75 neurotrophin receptor (p75NTR) in regulating the mouse alveolar bone development and the mineralization potential of murine ectomesenchymal stem cells (EMSCs). Moreover, we tried to explore the underlying mechanisms associated with the PI3K/Akt/ß-catenin pathway. MATERIALS AND METHODS: p75NTR knockout (p75NTR-/- ) mice and wild-type (WT) littermates were used. E12.5d p75NTR-/- and WT EMSCs were isolated in the same pregnant p75NTR-/+ mice from embryonic maxillofacial processes separately. Mouse alveolar bone mass was evaluated using micro-CT. Differential osteogenic differentiation pathways between p75NTR-/- and WT EMSCs were analysed by RNA-sequencing. The PI3K inhibitor LY294002 and PI3K agonist 740Y-P were used to regulate the PI3K/Akt pathway in EMSCs. p75NTR overexpression lentiviruses, p75NTR knock-down lentiviruses and recombined mouse NGF were used to transfect cells. RESULTS: The alveolar bone mass was found reduced in the p75NTR knockout mouse comparing to the WT mouse. During mineralization induction, p75NTR-/- EMSCs displayed decreased osteogenic capacity and downregulated PI3K/Akt/ß-catenin signalling. The PI3K/Akt/ß-catenin pathway positively regulates the potential of differential mineralization in EMSCs. The promotive effect of p75NTR overexpression can be attenuated by LY294002, while the inhibitory effect of p75NTR knock-down on Runx2 and Col1 expression can be reversed by 740Y-P. CONCLUSION: Deletion of p75NTR reduced alveolar bone mass in mice. P75NTR positively regulated the osteogenic differentiation of EMSCs via enhancing the PI3K/Akt/ß-catenin pathway.
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Pérdida de Hueso Alveolar/genética , Enfermedades Mandibulares/genética , Receptores de Factor de Crecimiento Nervioso/genética , Transducción de Señal , Pérdida de Hueso Alveolar/metabolismo , Pérdida de Hueso Alveolar/patología , Animales , Células Cultivadas , Eliminación de Gen , Masculino , Enfermedades Mandibulares/metabolismo , Enfermedades Mandibulares/patología , Ratones , Ratones Noqueados , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Receptores de Factor de Crecimiento Nervioso/metabolismo , beta Catenina/metabolismoRESUMEN
BACKGROUND: Certain constitutive chromosomal abnormalities of the human X chromosome are relatively common in conspicuous neuropsychiatric findings. Although tumors or tumor-like lesions are occasionally reported in diseases of the X chromosome, they are numerically negligible, for example, in aneuploidy such as the triple X syndrome (TXS). CASE REPORT: A 16-year-old female patient with a known TXS and premutation stage of fragile X syndrome was referred by her dentist for diagnosis and treatment of unilateral cheek swelling. The examination of the psychologically conspicuous patient revealed a unilateral mandibular tumor with dysesthesia of the mental nerve. Surgical removal of soft, crumbly spongiosa over the nerve canal resulted in sufficient pressure release of the constricted nerve and restoration of epicritic sensitivity. Imaging findings and histological and molecular genetic examination revealed monostotic craniofacial fibrous dysplasia. CONCLUSION: Although the data in the literature do not give reason to suppose an accumulation of neoplasms in TXS, a numb chin syndrome should be a reason for detailed diagnostics. Careful diagnosis allows for customized therapy. This is the first report on the coincidence of TXS, fragile X syndrome, and fibrous dysplasia in a single individual.
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Displasia Fibrosa Poliostótica/complicaciones , Síndrome del Cromosoma X Frágil/complicaciones , Enfermedades Mandibulares/complicaciones , Adolescente , Femenino , Displasia Fibrosa Poliostótica/diagnóstico por imagen , Displasia Fibrosa Poliostótica/genética , Síndrome del Cromosoma X Frágil/psicología , Humanos , Enfermedades Mandibulares/diagnóstico por imagen , Enfermedades Mandibulares/genética , FenotipoRESUMEN
Mandibular disorders are among the most common birth defects in humans, yet the etiological factors are largely unknown. Most of the neonates affected by mandibular abnormalities have a sequence of secondary anomalies, including airway obstruction and feeding problems, that reduce the quality of life. In the event of lacking corrective surgeries, patients with mandibular congenital disorders suffer from additional lifelong problems such as sleep apnea and temporomandibular disorders, among others. The goal of this systematic review is to gather evidence on hormonal and genetic factors that are involved in signaling pathways and interactions that are potentially associated with the nonsyndromic mandibular disorders. We found that members of FGF and BMP pathways, including FGF8/10, FGFR2/3, BMP2/4/7, BMPR1A, ACVR1, and ACVR2A/B, have a prominent number of gene-gene interactions among all identified genes in this review. Gene ontology of the 154 genes showed that the functional gene sets are involved in all aspects of cellular processes and organogenesis. Some of the genes identified by the genome-wide association studies of common mandibular disorders are involved in skeletal formation and growth retardation based on animal models, suggesting a potential direct role as genetic risk factors in the common complex jaw disorders. Developmental Dynamics 248:162-172, 2019. © 2018 Wiley Periodicals, Inc.
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Estudio de Asociación del Genoma Completo , Mandíbula/anomalías , Enfermedades Mandibulares/etiología , Proteínas Morfogenéticas Óseas/metabolismo , Factores de Crecimiento de Fibroblastos/metabolismo , Redes Reguladoras de Genes , Hormonas/farmacología , Humanos , Enfermedades Mandibulares/congénito , Enfermedades Mandibulares/genética , Transducción de SeñalRESUMEN
Primary aneurysmal bone cyst (ABC) is a cystic bone neoplasm characterized by disease-defining gene fusions involving the USP6/Tre2 gene. The literature describing gnathic ABC is limited. This case report describes a 27-year-old man presenting with a long-standing left-sided facial asymmetry. Multi-detector computed tomography imaging demonstrated a large expansile lesion positioned within the left condylar head. The lesion was biopsied and resected. The specimen showed a giant cell-rich cystic neoplasm, with fibrous tissue lined by multinucleated giant cells. Next-generation sequencing confirmed the presence of a USP6-CDH11 fusion gene, consistent with classification as a primary ABC, the first reported to be translocation-positive in the head of the mandibular condyle.
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Quistes Óseos Aneurismáticos/genética , Cadherinas/genética , Enfermedades Mandibulares/genética , Proteínas Proto-Oncogénicas/genética , Ubiquitina Tiolesterasa/genética , Adulto , Quistes Óseos Aneurismáticos/patología , Humanos , Masculino , Cóndilo Mandibular/patología , Enfermedades Mandibulares/patología , Translocación GenéticaRESUMEN
In literature there are few reports about multiple CGCG. But this is the first report of bilateral CGCG of the mandibular angles in three females from the same family.This report describes three cases of females from the same family - a mother and two young daughters - with bilateral CGCG in their jaw angles. All the lesions were surgically removed and the histopathologic diagnosis was always identical: giant cell central granulomas, with patterns that were absolutely superimposable between them and with that of the mother.The hypothesis is that this presentation of CGCG may be defined as hereditary bilateral CGCG of the mandibular angles (or also, cherubism-like lesions).
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Querubismo/genética , Predisposición Genética a la Enfermedad , Granuloma de Células Gigantes/genética , Enfermedades Mandibulares/genética , Monitoreo Fisiológico/métodos , Biopsia con Aguja , Niño , Femenino , Granuloma de Células Gigantes/diagnóstico por imagen , Granuloma de Células Gigantes/patología , Granuloma de Células Gigantes/cirugía , Humanos , Inmunohistoquímica , Enfermedades Mandibulares/diagnóstico por imagen , Enfermedades Mandibulares/patología , Enfermedades Mandibulares/cirugía , Persona de Mediana Edad , Procedimientos Quirúrgicos Orales/métodos , Linaje , Pronóstico , Radiografía Panorámica/métodos , Enfermedades Raras , Medición de RiesgoRESUMEN
OBJECTIVES: Skeletal mandibular hypoplasia (SMH), a common type of developmental deformities, results in impaired aesthetics of facial profile, occlusal dysfunction and poor life quality. In this study, BMAL1 deficiency leads to SMH formation, and we aim to investigate the mechanism by which BMAL1 deficiency induces SMH. MATERIALS AND METHODS: Circadian rhythm-disordered mouse models were constructed by placing animals in a jet lag schedule of 6-h light advance every 7 days for 4 or 8 weeks. The OPG expression was evaluated by histomorphometry, immunohistochemistry and western blot analysis. The mechanism by which BMAL1 affects OPG expression was investigated by chromatin immunoprecipitation and luciferase reporter assays. The phenotypes caused by BMAL1 knockout can be rescued by exogenous supplementation with OPG. RESULTS: We demonstrate that the expressions of BMAL1 and OPG decreased in SMH patients. Circadian rhythm-disordered mice and Bmal1-/- mice exhibited decreased expression of OPG, reduced bone mass and bone size of mandibles. Our results revealed that BMAL1 bound directly to the Opg promoter and upregulated its expression, thus inhibiting osteoclast differentiation. BMAL1 deficiency increased osteoclast differentiation by downregulating OPG expression. In vitro, the enhancement effect of osteoclast differentiation caused by BMAL1 knockdown was significantly reversed by exogenous supplementation with OPG. Importantly, bone loss caused by BMAL1 knockout can be partially reversed by injecting OPG Intraperitoneally. CONCLUSIONS: These results indicate that the circadian clock plays a critical role in the growth and development of mandible by regulating OPG expression, and present a potential therapeutic strategy to prevent SMH.
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Factores de Transcripción ARNTL/deficiencia , Factores de Transcripción ARNTL/genética , Anomalías Craneofaciales/genética , Regulación hacia Abajo/genética , Enfermedades Mandibulares/genética , Osteoprotegerina/genética , Animales , Diferenciación Celular/genética , Niño , Relojes Circadianos/genética , Humanos , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Músculo Esquelético/metabolismo , Osteoclastos/metabolismo , Regiones Promotoras Genéticas/genética , Regulación hacia Arriba/genéticaRESUMEN
OBJECTIVE: Fibrous dysplasia (FD) is a benign bone disease characterized by fibro-osseous lesions. FD is caused by somatic mutations in the gene, guanine nucleotide-binding protein, alpha stimulating activity polypeptide 1 (GNAS), which encodes the G protein subunit, Gsα. FD manifests early in life, but the growth of lesions usually ceases in adulthood. FD lesions often exhibit somatic mutation mosaicism. In this study, the relationship between lesion growth and mutation prevalence within a lesion was investigated. DESIGN: Lesions from five FD patients were characterized by radiographical, histological and immunohistochemical methods. To accurately calculate the prevalence of mutations within lesions, GNAS codon 201 in genomic DNA isolated from fresh surgical FD specimens was sequenced. RESULTS: Uniquely, a lesion in one 46-year-old patient was still growing, enabling simultaneous analysis of both stable-old and active-new FD lesions in the same patient. Immunohistochemical analysis indicated that a newer, proximal lesion was growing while an older, distal lesion was not. The mutation prevalence differed between these lesions; it was low in the old and high in the new lesion. Thus, the frequency of mutated cells had decreased in the older lesion. CONCLUSIONS: This is the first direct evidence for the age-dependent demise of mutated cells in FD, helping to explain why FD lesion growth generally ceases in adulthood.
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Cromograninas/genética , Displasia Fibrosa Ósea/genética , Subunidades alfa de la Proteína de Unión al GTP Gs/genética , Enfermedades Mandibulares/genética , Adulto , Factores de Edad , Análisis Mutacional de ADN , Femenino , Displasia Fibrosa Ósea/diagnóstico por imagen , Displasia Fibrosa Ósea/cirugía , Humanos , Inmunohistoquímica , Masculino , Enfermedades Mandibulares/diagnóstico por imagen , Enfermedades Mandibulares/cirugía , Radiografía Panorámica , ReoperaciónRESUMEN
Skeletal mandibular hypoplasia (SMH), one of the common types of craniofacial deformities, seriously affects appearance, chewing, pronunciation, and breathing. Moreover, SMH is prone to inducing obstructive sleep apnea syndrome. We found that brain and muscle ARNT-like 1 (BMAL1), the core component of the molecular circadian oscillator, was significantly decreased in mandibles of juvenile SMH patients. Accordingly, SMH was observed in circadian-rhythm-disrupted or BMAL1-deficient mice. RNA sequencing and protein chip analyses suggested that matrix metallopeptidase 3 (MMP3) is the potential target of BMAL1. Interestingly, in juvenile SMH patients, we observed that MMP3 was obviously increased. Consistently, MMP3 was upregulated during the whole growth period of 3-10 weeks in Bmal1-/- mice. Given these findings, we set out to characterize the underlying mechanism and found BMAL1 deficiency enhanced Mmp3 transcription through activating p65 phosphorylation. Together, our results provide insight into the mechanism by which BMAL1 is implicated in the pathogenesis of SMH.
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Factores de Transcripción ARNTL/deficiencia , Regulación Enzimológica de la Expresión Génica , Enfermedades Mandibulares/metabolismo , Metaloproteinasa 3 de la Matriz/biosíntesis , Regulación hacia Arriba , Animales , Niño , Femenino , Humanos , Masculino , Enfermedades Mandibulares/genética , Enfermedades Mandibulares/patología , Metaloproteinasa 3 de la Matriz/genética , Ratones , Ratones NoqueadosRESUMEN
BACKGROUND: Inflammatory myofibroblastic tumor (IMT) is a rare low-grade malignant neoplasm with a predilection for children and young adults, and typically arises in the lung, abdominopelvic region, and retroperitoneum. IMTs in the maxillofacial region are extreme rare. Approximately 50% of IMT harbor rearrangements of the anaplastic lymphoma kinase (ALK) gene at 2p23 with various fusion partners. CASE PRESENTATION: We herein report a case of intraosseous IMT of the mandible with a novel ATIC-ALK fusion. Tooth 43 did not erupt after the loss of tooth 83 in an 11-year-old girl with no previous history of trauma. Panoramic tomography showed a unilocular radiolucent lesion in the right anterior mandible resorbing the root of tooth 42 and the medial side of the root of tooth 44. Computed tomography revealed a well- circumscribed 3-cm osteolytic lesion of the right anterior mandible eroding the buccal cortical plate. The entire lesion was curetted out. A histopathological examination revealed the proliferation of plump spindle cells with a storiform architecture and lymphocytes scattered around spindle cells. The spindle cells showed diffuse cytoplasmic staining for ALK by immunohistochemistry. A fluorescence in situ hybridization analysis revealed the translocation of a part of the ALK gene locus at chromosome 2p23. A rapid amplification of cDNA ends analysis confirmed the rearrangement of ALK and identified ATIC as a partner of this ALK fusion mutant. CONCLUSION: To the best of our knowledge, this is the first case of intraosseous IMT of the mandible with a novel ATIC-ALK fusion. We also herein reviewed similar tumors reported in the literature.
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Granuloma de Células Plasmáticas/genética , Enfermedades Mandibulares/genética , Proteínas de Fusión Oncogénica/genética , Biomarcadores de Tumor/análisis , Niño , Femenino , Granuloma de Células Plasmáticas/patología , Humanos , Inmunohistoquímica , Hibridación Fluorescente in Situ , Inflamación/genética , Inflamación/patología , Enfermedades Mandibulares/patologíaRESUMEN
BACKGROUND: Mandibular coronoid hyperplasia is a rare condition associated with gradual reduction in mouth opening. Its aetiology is unknown but increased temporalis activity, endocrine stimulus, trauma and familial causes have been proposed. CASE REPORT: In this article, we present a case of bilateral coronoid hyperplasia presenting with limited mouth opening in 16-year-old Caucasian twin sisters. DISCUSSION: This report gives a new perspective to the aetiology of coronoid hyperplasia since it describes its occurrence in monozygotic twins and hence provides a strong argument in support of a genetic aetiology.
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Enfermedades en Gemelos/genética , Enfermedades Mandibulares/genética , Trastornos de la Articulación Temporomandibular/genética , Articulación Temporomandibular/patología , Trismo/genética , Gemelos Monocigóticos/genética , Adolescente , Enfermedades en Gemelos/diagnóstico por imagen , Enfermedades en Gemelos/patología , Enfermedades en Gemelos/cirugía , Femenino , Humanos , Hiperplasia , Imagenología Tridimensional , Maloclusión Clase II de Angle/diagnóstico por imagen , Maloclusión Clase II de Angle/genética , Enfermedades Mandibulares/diagnóstico por imagen , Enfermedades Mandibulares/patología , Enfermedades Mandibulares/cirugía , Articulación Temporomandibular/diagnóstico por imagen , Articulación Temporomandibular/cirugía , Trastornos de la Articulación Temporomandibular/diagnóstico por imagen , Trastornos de la Articulación Temporomandibular/patología , Trastornos de la Articulación Temporomandibular/cirugía , Tomografía Computarizada por Rayos X , Trismo/diagnóstico por imagen , Trismo/patología , Trismo/cirugíaRESUMEN
Giant cell granuloma (GCG) of the jaw is a rare, well-known feature of neurofibromatosis type 1 (NF1), an inborn multisystem disorder. Recently, the development of GCG in NF1 was attributed to second hit mutations in the NF1 gene. The treatment of GCG is pragmatic with a preference for local curettage of lytic osseous areas. This report describes the surgical therapy of an NF1-affected female with multilocular mandibular GCG and hypodontia who additionally suffered from a brain tumour and Hashimoto's thyroiditis. Although local recurrence of GCG was noted, augmentation of the curetted cavities with a bone substitute in successive interventions successfully restored the extensive periradicular local defects and stabilised the teeth. A meticulous in vitro study of the GCG specimen revealed a second hit mutation in the NF1 gene in the GCG spindle-cells. This study contributes to the increasing knowledge of the molecular basis for GCG in the jaw of NF1 patients, indicating that it is a neoplasm.
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Genes de Neurofibromatosis 1 , Granuloma de Células Gigantes/genética , Enfermedades Mandibulares/genética , Mutación , Adolescente , Sustitutos de Huesos , Neoplasias Encefálicas/complicaciones , Tomografía Computarizada de Haz Cónico , Femenino , Granuloma de Células Gigantes/diagnóstico por imagen , Granuloma de Células Gigantes/cirugía , Humanos , Procesamiento de Imagen Asistido por Computador , Enfermedades Mandibulares/diagnóstico por imagen , Enfermedades Mandibulares/cirugía , Neurofibromatosis 1/complicaciones , Neurofibromatosis 1/genética , Radiografía Panorámica , RecurrenciaAsunto(s)
Enfermedades del Desarrollo Óseo/genética , Cardiomiopatía Dilatada/genética , Lamina Tipo A/genética , Lipodistrofia/genética , Enfermedades Mandibulares/genética , Mutación/genética , Fenotipo , Adulto , Enfermedades del Desarrollo Óseo/complicaciones , Enfermedades del Desarrollo Óseo/diagnóstico , Cardiomiopatía Dilatada/complicaciones , Cardiomiopatía Dilatada/diagnóstico , Humanos , Lipodistrofia/complicaciones , Lipodistrofia/diagnóstico , Masculino , Enfermedades Mandibulares/complicaciones , Enfermedades Mandibulares/diagnósticoAsunto(s)
Síndrome del Nevo Basocelular/genética , Síndrome del Nevo Basocelular/patología , Membrana Epirretinal/patología , Enfermedades Mandibulares/patología , Enfermedades Maxilares/patología , Quistes Odontogénicos/patología , Receptor Patched-1/genética , Niño , Membrana Epirretinal/genética , Femenino , Mutación del Sistema de Lectura , Eliminación de Gen , Humanos , Enfermedades Mandibulares/genética , Enfermedades Maxilares/genética , Quistes Odontogénicos/genéticaAsunto(s)
Enfermedades Mandibulares/diagnóstico por imagen , Osteopetrosis/diagnóstico por imagen , Adolescente , Densidad Ósea , Medios de Contraste , Diagnóstico Diferencial , Femenino , Humanos , Masculino , Enfermedades Mandibulares/genética , Osteopetrosis/genética , Radiografía Panorámica , Tomografía Computarizada por Rayos XRESUMEN
BACKGROUND: Lipodystrophies are a large heterogeneous group of genetic or acquired disorders characterized by generalized or partial fat loss, usually associated with metabolic complications such as diabetes mellitus, hypertriglyceridemia and hepatic steatosis. Many efforts have been made in the last years in identifying the genetic etiologies of several lipodystrophy forms, although some remain to be elucidated. METHODS: We report here the clinical description of a woman with a rare severe lipodystrophic and progeroid syndrome associated with hypertriglyceridemia and diabetes whose genetic bases have been clarified through whole-exome sequencing (WES) analysis. RESULTS: This article reports the 5th MDPL (Mandibular hypoplasia, deafness, progeroid features, and lipodystrophy syndrome) patient with the same de novo p.S605del mutation in POLD1. We provided further genetic evidence that this is a disease-causing mutation along with a plausible molecular mechanism responsible for this recurring event. Moreover we overviewed the current classification of the inherited forms of lipodystrophy, along with their underlying molecular basis. CONCLUSIONS: Progress in the identification of lipodystrophy genes will help in better understanding the role of the pathways involved in the complex physiology of fat. This will lead to new targets towards develop innovative therapeutic strategies for treating the disorder and its metabolic complications, as well as more common forms of adipose tissue redistribution as observed in the metabolic syndrome and type 2 diabetes.
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Sordera/genética , Lipodistrofia/fisiopatología , Enfermedades Mandibulares/genética , Adulto , Femenino , Humanos , Lipodistrofia/genética , Masculino , Linaje , SíndromeRESUMEN
Periodontal disease (Periodontitis) is a serious disease that affects a majority of adult Americans and is associated with other systemic diseases, including diabetes, rheumatoid arthritis, and other inflammatory diseases. While great efforts have been devoted toward understanding the pathogenesis of periodontitis, there remains a pressing need for developing potent therapeutic strategies for targeting this pervasive and destructive disease. In this study, we utilized novel adeno-associated virus (AAV)-mediated Atp6v1c1 knockdown gene therapy to treat bone erosion and inflammatory caused by periodontitis in mouse model. Atp6v1c1 is a subunit of the V-ATPase complex and regulator of the assembly of the V0 and V1 domains of the V-ATPase complex. We demonstrated previously that Atp6v1c1 has an essential function in osteoclast mediated bone resorption. We hypothesized that Atp6v1c1 may be an ideal target to prevent the bone erosion and inflammation caused by periodontitis. To test the hypothesis, we employed AAV RNAi knockdown of Atp6v1c1 gene expression to prevent bone erosion and gingival inflammation simultaneously. We found that lesion-specific injection of AAV-shRNA-Atp6v1c1 into the periodontal disease lesions protected against bone erosion (>85%) and gingival inflammation caused by P. gingivalis W50 infection. AAV-mediated Atp6v1c1 knockdown dramatically reduced osteoclast numbers and inhibited the infiltration of dendritic cells and macrophages in the bacteria-induced inflammatory lesions in periodontitis. Silencing of Atp6v1c1 expression also prevented the expressions of osteoclast-related genes and pro-inflammatory cytokine genes. Our data suggests that AAV-shRNA-Atp6v1c1 treatment can significantly attenuate the bone erosion and inflammation caused by periodontitis, indicating the dual function of AAV-shRNA-Atp6v1c1 as an inhibitor of bone erosion mediated by osteoclasts, and as an inhibitor of inflammation through down-regulation of pro-inflammatory cytokine expression. This study demonstrated that Atp6v1c1 RNAi knockdown gene therapy mediated by AAV-shRNA-Atp6v1c1 is a promising novel therapeutic approach for the treatment of bone erosion and inflammatory related diseases, such as periodontitis and rheumatoid arthritis.
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Pérdida de Hueso Alveolar/genética , Inflamación/genética , Enfermedades Mandibulares/genética , Periodontitis/genética , ATPasas de Translocación de Protón Vacuolares/genética , Pérdida de Hueso Alveolar/inmunología , Pérdida de Hueso Alveolar/metabolismo , Pérdida de Hueso Alveolar/terapia , Animales , Modelos Animales de Enfermedad , Femenino , Terapia Genética , Inflamación/inmunología , Inflamación/metabolismo , Inflamación/terapia , Enfermedades Mandibulares/inmunología , Enfermedades Mandibulares/metabolismo , Enfermedades Mandibulares/terapia , Ratones , Ratones Endogámicos BALB C , Ratones Noqueados , Osteoclastos/efectos de los fármacos , Osteoclastos/fisiología , Periodontitis/inmunología , Periodontitis/metabolismo , Periodontitis/terapia , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/farmacología , ATPasas de Translocación de Protón Vacuolares/antagonistas & inhibidoresRESUMEN
OBJECTIVE: The aim of this study is to investigate the multifactorial etiology of mandibular tori analyzing the influence of genetics, occlusal overload, various clinical variables and their interactions. METHODS: Overall, plaster casts of 162 twins (81 twin pairs) were analyzed for the presence or absence of mandibular tori. Atypical wear facets on canine tips or incisors were recorded to diagnose bruxism. Angle Class, any kind of anterior open bite and positive, negative or flat curve of Wilson were recorded. Zygosity determination was carried out using a DNA test. RESULTS: Mandibular tori were found in 56.8% of the cases. In 93.6% of all monozygotic twin pairs both individuals had or did not have mandibular tori (κ=0.96±0.04; p<0.001), compared to 79.4% concordance of mandibular tori in dizygotic co-twins (κ=0.7±0.12; p<0.001). Prevalence of mandibular tori was significantly higher in the group of bruxers (67.5%) compared to non-bruxers (31.3%) (p<0.001). Significant association between mandibular tori and negative or flat curve of Wilson in the maxillary second premolars and first molars was found (OR=2.55, 95% CI (1.19-5.46), p=0.016). In all monozygotic bruxers, 97.1% showed concordance of mandibular tori presence in both co-twins compared to 78.9% dizygotic bruxers, and this difference is statistically significant (p=0.007). CONCLUSION: Our results suggest that the mandibular tori are of a multifactorial origin. Mandibular tori seem to have genetic predisposition, and may be associated with teeth grinding as well as with negative or flat CW in region of maxillary second premolar and first molar.