Diurnal raptors at rescue centres in the Czech Republic: Reasons for admission, outcomes, and length of stay.

Rescue centres play an important role in the protection of raptors living in the wild by caring for injured or debilitated animals and abandoned young with the aim of returning them to the wild. A total of 22,538 raptors were admitted to 34 rescue centres in the Czech Republic in the years 2010-2019, with an increasing trend during the monitored period (rSp = 0.7333, p < 0.05). The most frequent reasons for their admission were other injuries and fractures (26.52%), the admission of young (22.98%), and the admission of raptors injured by electric shock injuries (20.51%). It proved possible to release 42.45% of admitted raptors back into the wild, the majority of which (91.05%) were released using the hard-release method. Foster parents were used in 1% of cases and a replacement nest in 0.2% of cases involving the rearing of young. In spite of all the care provided at rescue centres, a total of 39.97% of raptors admitted either died or had to be euthanized. Among them, most raptors were euthanized or died due to injuries caused by collision with a vehicle, electric shock injuries, and other injuries. This generally occurred shortly after admission (a median of two days). The importance of the work of rescue centres lies not merely in returning injured raptors back into the wild (which proves possible in around half of all cases), but also in obtaining information about the factors endangering raptors in the wild and contributing toward a decline in their populations. The findings provide information about human-wildlife interactions in the Czech Republic and their implications for conservation as well as on the effectiveness of rescue centres to successfully treat and subsequently release raptors back into the wild.

Outcomes of Birds of Prey with Surgical or Traumatic Wing Amputation: A Retrospective Study from 1995 to 2017.

Surgical amputation of a limb is often required to treat raptor orthopedic injuries at rehabilitation centers. In some cases, amputation is an alternative to euthanasia if the bird's welfare is deemed appropriate under human care. The outcome for raptors maintained in a captive setting following wing amputation is poorly documented. A retrospective study was conducted in a Canadian raptor rehabilitation facility to assess the outcomes and complications observed in captive and free-living raptors with partial or complete, surgical or traumatic amputations of the wing. Data from raptors admitted to the rehabilitation center from 1995 to 2017 were reviewed. Overall, 32 records were included in this retrospective study from 11 species of raptors with surgical or traumatic wing amputations. Survival times of the 23 birds with an amputated wing (median: 1070 days, range: 68 days to 13 years and 1 month) were significantly (P = .02) longer than the survival times of the 404 non-amputated birds (median: 696 days, range 37 days to 27 years and 3 months). Complications occurred in 13 of 30 birds (43%) during the recovery period, with 9 of 30 (30%) birds developing life-threatening complications. Maladaptation to captivity was the leading cause of euthanasia during this period. After placement in captivity, 3/23 (13%) birds developed complications related to the amputation site. Based on this study, we conclude that some birds can tolerate partial or complete wing amputation, but the decision to place a bird in a captive setting should encompass the bird's ability to cope with human interaction and the availability of an adapted and safe enclosure for the animal.

Nutritional Secondary Hyperparathyroidism and Fibrous Osteodystrophy in a Captive African Penguin (Spheniscus demersus) Similar to Osteomalacia in Poultry.

A 9-yr-old female black-footed African penguin (Spheniscus demersus) was presented for necropsy after a history of reproductive abnormalities, paresis of limbs, weakness, and sudden death. Postmortem examination revealed soft keel, collapsed rib cage with beading of the ribs, and bilateral parathyroid enlargement. Classic histologic lesions of fibrous osteodystrophy with osteomalacia were observed in the ribs, vertebrae, and to a lesser extent in the femur and tibiotarsus associated with hyperplasia of parathyroid glands. This represents the first report of nutritional secondary hyperparathyroidism in birds of the order Spheniciformes, most likely caused by low levels of calcium supplementation during egg laying. The reproductive abnormalities observed in this penguin and others from the same group (asynchronous egg-laying cycles, abnormal breeding behavior) were most likely exacerbated by the lack of an adequate photoperiod mimicking the natural daylight pattern.

Reporte de caso­Hiperparatiroidismo secundario nutricional y osteodistrofia fibrosa en un pingüino africano (Spheniscus demersus) en cautiverio similar a la osteomalacia observada en de aves de corral. Una hembra de pingüino africano de patas negras (Spheniscus demersus) de nueve años fue sometida a necropsia después de un historial de anomalías reproductivas, paresia de extremidades, debilidad y muerte súbita. El examen post mortem reveló que la quilla del esternón estaba blanda, la caja torácica colapsada, se observaron "perlas raquíticas" en las costillas y agrandamiento bilateral de las paratiroides. Se observaron lesiones histológicas clásicas de osteodistrofia fibrosa con osteomalacia en las costillas, vértebras y en menor medida, en el fémur y tibiotarsus asociadas con hiperplasia de glándulas paratiroides. Esto representa el primer informe de hiperparatiroidismo secundario nutricional en un ave del orden Spheniciformes, muy probablemente causado por un bajo nivel de suplementos de calcio durante la producción de huevos. Las anomalías reproductivas observadas en este pingüino y otros del mismo grupo (ciclos de puesta de huevos asincrónicos, comportamiento de reproducción anormal) probablemente se vieron exacerbadas por la falta de un fotoperíodo adecuado que imitara el patrón de luz natural.

Bird-feeder cleaning lowers disease severity in rural but not urban birds.

Animals inhabiting urban areas often experience elevated disease threats, putatively due to factors such as increased population density and horizontal transmission or decreased immunity (e.g. due to nutrition, pollution, stress). However, for animals that take advantage of human food subsidies, like feeder-visiting birds, an additional mechanism may include exposure to contaminated feeders as fomites. There are some published associations between bird feeder presence/density and avian disease, but to date no experimental study has tested the hypothesis that feeder contamination can directly impact disease status of visiting birds, especially in relation to the population of origin (i.e. urban v. rural, where feeder use/densities naturally vary dramatically). Here we used a field, feeder-cleaning experimental design to show that rural, but not urban, house finches (Haemorhous mexicanus) showed increased infection from a common coccidian endoparasite (Isospora spp.) when feeders were left uncleaned and that daily cleaning (with diluted bleach solution) over a 5-week period successfully decreased parasite burden. Moreover, this pattern in rural finches was true for males but not females. These experimental results reveal habitat- and sex-specific harmful effects of bird feeder use (i.e. when uncleaned in rural areas). Our study is the first to directly indicate to humans who maintain feeders for granivorous birds that routine cleaning can be critical for ensuring the health and viability of visiting avian species.

Atypical behavior of a black fly species connects cavity-nesting birds with generalist blood parasites in an arid area of Spain.

BACKGROUND: The feeding behavior of bloodsucking insects determines the transmission, distribution, host spectrum and evolution of blood parasites in the wild. Conventional wisdom suggests that some vector groups (e.g. black flies, family Simuliidae) are consistently exophagous daytime biters. We aimed to understand more about the exceptions to this pattern by combining targeted trapping and molecular identification of parasites in vectors. METHODS: In this study, we collected black flies in nest boxes used by European rollers Coracias garrulus in southeastern Spain. We molecularly analyzed 434 individual insects, identifying the black fly species caught in the nest boxes, their potential vertebrate blood meals, and the haemosporidian parasite lineages that they carried. RESULTS: Only one black fly species, Simulium rubzovianum, appeared to enter the nest boxes of rollers. Among the trapped specimens, 15% contained vertebrate DNA, which always belonged to rollers, even though only half of those specimens were visibly engorged. Furthermore, 15% of all black flies contained Leucocytozoon lineages, indicating previous feeding on avian hosts but probably not on infected adult rollers. The known vertebrate hosts of the recorded Leucocytozoon lineages suggested that large and/or abundant birds are their hosts. Particularly represented were cavity-nesting species breeding in the vicinity, such as pigeons, corvids and owls. Open-nesting species such as thrushes and birds of prey were also represented. CONCLUSIONS: Our data strongly suggest that S. rubzovianum bites uninfected roller nestlings and infected individuals of other species, potentially incubating adults, inside nest boxes and natural cavities. This simuliid does not appear to have a strong preference for specific host clades. Contrary to the general pattern for the group, and possibly enhanced by the harsh environmental conditions in the study area, this black fly appeared to intensively use and may even have a preference for confined spaces such as cavities for feeding and resting. Preferences of vectors for atypical microhabitat niches where hosts are less mobile may enable social and within-family transmission and parasite speciation in the long term. At the same time, a lack of host preference in concentrated multispecies communities can lead to host switches. Both processes may be underappreciated driving forces in the evolution of avian blood parasites.

A RETROSPECTIVE STUDY OF FEMORAL FRACTURES IN WILD BIRDS OF PREY: 119 CASES.

A retrospective study was performed to describe patient and fracture characteristics, treatments, complications, and outcomes associated with femoral fractures in free-ranging birds of prey. Cases were identified from the electronic database and included raptors admitted with femoral fractures at the Bird of Prey Clinic of the Université de Montréal, from 1986 to 2018. In total, 119 individuals from 24 species, with 123 femoral fractures, were included in the study. Twenty-seven birds (23%) were declared dead upon arrival. Ninety-two birds (77%) were admitted alive; 80 with acute fractures and 12 with a fracture that had already healed. A conservative treatment (cage rest) was used to manage eight fractures. Surgical repair was attempted on 34 femoral fractures in 33 birds. An intramedullary pin alone was used in 28 cases (82%), and an external skeletal fixator-intramedullary pin tie-in fixator was used in 6 cases (18%). For surgically treated fractures, 19 healed (in 18 birds), 4 did not heal, and 11 birds died or were euthanized before healing could be achieved. Of the 23/34 fractures where the healing process could be assessed, healing rate for comminuted fractures (11/23) was 91% (10/11). Surgical complications occurred in 10 cases (including 7 cases of migration and loosening of the pin, and 1 case of osteomyelitis), leading to euthanasia in 4 cases. Out of the 21 birds with acute femoral fracture upon presentation that were released, 14 had received a surgical treatment and 7 had received a conservative treatment. Nine of the 21 released birds (43%) had at least one other fractured bone. The majority of deaths and euthanasia occurred within 2 wk after admission (51/59; 86%). Thirteen birds were euthanized primarily because of their femoral fracture. Thirteen birds died in treatment and 33 were deemed nonreleasable and euthanized because of a comorbidity not related to the femoral fracture.

CLINICAL OBSERVATIONS OF BEE ENVENOMATION IN TOUCANS.

There are numerous reports on envenomation, even fatal, secondary to bee attacks in humans and other mammals. In birds, reports on those incidents are scarce and there are none regarding honeybee (Apis mellifera) stings in toucans. In the first case presented, an adult female red-breasted toucan (Ramphastos dicolorus) received at least five bee stings in the periophthalmic area. Within 5 h the bird was lethargic and dehydrated. The urates were yellowish. Three days later the bird showed a moderate anemia, but no changes in the leukocyte count, beyond an elevated heterophil: lymphocyte ratio. Blood chemistry showed hyperglycemia, hypoalbuminemia and elevated aspartate aminotransferase and creatine kinase. Alterations in electrolyte values were also noted. Fourteen days later bile acid elevation was observed. Hematocrit levels normalized after 2 wk. A second incident involved a breeding pair of toco toucans (Ramphastos toco). While the female toco toucan received 10 stings and showed mild clinical manifestations, the male toco toucan was more severely attacked, receiving 40 stings, and died overnight. Despite the relative gravity of the attack (in terms of number of stingers in relation to body weight) both surviving birds recovered in less than 2 wk. To the authors' knowledge, fatal bee envenomation in birds has been reported only in pigeons and macaws. The findings described in this report suggest that toucans are less sensitive to bee venom when compared with pigeons and may have higher tolerance to bee venom compared with mammals. Honeybee envenomation must be considered a potential threat when considering toucan husbandry in zoos and collections.

Evaluation of high-resolution melt curve analysis for rapid differentiation of Campylobacter hepaticus from other species in birds.

Spotty liver disease (SLD) is a bacterial disease of chicken, causing mortalities and reduction in egg production, hence, contributing to economic loss in the poultry industry. The causative agent of SLD has only recently been identified as a novel Campylobacter species, Campylobacter hepaticus. Specific primers were designed from the hsp60 gene of Campylobacter hepaticus and PCR followed by high-resolution melt curve analysis was optimised to detect and differentiate three species of Campylobacter (Campylobacter coli, Campylobacter jejuni and Campylobacter hepaticus). The three Campylobacter species produced a distinct curve profile and was differentiated using HRM curve analysis. The potential of the PCR-HRM curve analysis was shown in the genotyping of 37 Campylobacter isolates from clinical specimens from poultry farms. PCR-HRM curve analysis of DNA extracts from bile samples or cultures from bile samples, were identified as Campylobacter hepaticus and confirmed by DNA sequencing. The DNA sequence analysis of selected samples from each of the three HRM distinctive curves patterns showed that each DNA sequence was associated with a unique melt profile. The potential of the PCR-HRM curve analysis in genotyping of Campylobacter species was also evaluated using faecal specimens from 100 wild birds. The results presented in this study indicate that PCR followed by HRM curve analysis provides a rapid and robust technique for genotyping of Campylobacter species using either bacterial cultures or clinical specimens.

Impact of high dietary energy on obesity and oxidative stress in domestic pigeons.

Obesity is associated with increased risk of oxidative stress in humans and laboratory animals but information regarding obesity-induced oxidative stress in birds is lacking. Therefore, this study aimed to investigate the influence of high-energy diets (HED) on obesity and oxidative stress in domestic pigeons. Forty-five adult clinically healthy-domestic male pigeons were randomly assigned to three equal dietary groups including low (2,850 kcal/kg), medium (3,150 kcal/kg) and high (3,450 kcal/kg) energy diets (named low energy diet, medium-energy diet and HED, respectively). All birds received formulated diets for 60 consecutive days. Several parameters such as feed intake, body weight (BW), average weight gain (AWG) and total weight gain were determined. Serum concentrations of triglyceride (TG), total cholesterol (TC), high-, low- and very-low-density lipoprotein cholesterols, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were evaluated at days 0, 30 and 60; and serum levels of total antioxidant capacity (T-AOC), malondialdehyde (MDA) and cortisol were also measured at day 60. On day 60, five pigeons from each group were randomly euthanized and some parameters such as weight and relative weight of liver, breast muscle, and abdominal fat were determined. Furthermore, hepatic total fat content was also evaluated. BW, AWG, total weight, and circulating TG, TC, ALT, AST, ALP, MDA and cortisol in HED were significantly higher than other groups. Serum T-AOC in HED was significantly lower than the other groups. In conclusion, this study showed that increasing dietary energy up to 3,450 kcal/kg in pigeons led to obesity and oxidative stress in them. Accordingly, it could be stated that HED and obesity induce oxidative stress in pigeon and controlling the dietary energy intake of pigeons is necessary to prevent oxidative stress in them.

PATHOLOGY OF HOUSE MOUSE (MUS MUSCULUS) PREDATION ON LAYSAN ALBATROSS (PHOEBASTRIA IMMUTABILIS) ON MIDWAY ATOLL NATIONAL WILDLIFE REFUGE.

Invasive rodents on islands have adverse effects on native birds in island ecosystems, and rats are the most common culprits. Recently, house mice (Mus musculus) in the South Atlantic were found preying on three species of albatross chicks. Here, we show that house mice can also prey on nesting adult Laysan Albatross (Phoebastria immutabilis) on Midway Atoll National Wildlife Refuge (US). In contrast to mouse attacks on albatross in the South Atlantic, where mice targeted the rump and crown of chicks, on Midway, mice targeted nesting adults mainly on the back. For both regions, the outcome was similar with reduced nesting success. In the case of Midway, reduced nesting success was due to nest abandonment or mortality of one or both parents because of secondary bacterial infections. Mouse-induced mortality of adult albatross has the potential to have a more potent demographic effect because of their much higher natural survivorship once they reach adulthood.

Current situation regarding lead exposure in birds in Japan (2015-2018); lead exposure is still occurring.

Birds of a number of species have died as a result of lead (Pb) poisoning, including many Steller's sea eagles (Haliaeetus pelagicus) and white-tailed sea eagles (Haliaeetus albicilla) in Hokkaido, the northernmost island of Japan. To address this issue, the use of any type of Pb ammunition for hunting of large animals was prohibited in Hokkaido in 2004. However, Pb poisoning is still being reported in this area, and there are few regulations regarding the use of Pb ammunition in other parts of Japan, where it has been reported that eagles and water birds have been exposed to Pb. This study was performed to accurately determine the current level of Pb exposure of birds found dead in the field or dead in the wild bird centers in Japan (June 2015-May 2018) and to identify the sources of Pb. Pb exposure was found to still be occurring in raptors and water birds in various parts of Japan. Twenty-six point five % and 5.9% of the recorded deaths of Steller's sea eagles and white-tailed sea eagles, respectively, were found to have been poisoned by Pb. In addition, Pb isotope ratio analysis showed that both Pb rifle bullets and Pb shot pellets cause Pb exposure in birds, and these endangered eagles are also exposed to Pb in Hokkaido due to the illegal use of Pb ammunition. Changing to Pb-free ammunition, such as copper (Cu) rifle bullets, steel shot pellets, or bismuth shot pellets, will be essential for the conservation of avian species in Japan.

Postmortem findings in Magellanic penguins (Spheniscus magellanicus) caught in a drift gillnet.

BACKGROUND: Penguin interaction with gillnets has been extensively reported in the Atlantic and Pacific Oceans, and is considered a major conservation threat. Among penguin species, Magellanic penguins (Spheniscus magellanicus) are currently considered of great concern, particularly in Brazil, where they are highly susceptible to gillnet bycatch. Nevertheless, information about drowning-associated microscopic findings in penguins is limited. RESULTS: We describe the anatomopathological findings of 20 Magellanic penguins that drowned after getting entangled in a drift gillnet while wintering along the Brazilian shelf and washed ashore still enmeshed in Santa Catarina, Brazil. All 20 birds (19 juveniles and 1 adult; 18 females and 2 males) were in good body condition. Major gross findings were abrasion, bruising, and local erythema and edema of the wings, multiorgan congestion, jugular vein engorgement, pulmonary edema and hemorrhage, splenomegaly and hepatomegaly, fluid in the trachea, serous bloody fluid in the lungs, gastrointestinal parasites (nematodes, cestodes and trematodes), and debris in the stomach. The most common histopathological findings were cerebral and pulmonary congestion, pulmonary edema, splenic histiocytosis, lymphoid splenic hyperplasia, acute splenitis, extramedullary hepatic hematopoiesis, and parasitic enteritis. Although unspecific, the observed multiorgan congestion and pulmonary edema are consistent with previous reports of drowning in birds and may be indicative of this process. CONCLUSIONS: Drowning may be a challenging diagnosis (e.g., carcass decomposition, predation), but must be considered as a differential in all beach-cast seabird postmortem examinations. To the authors' knowledge this is the largest anatomopathological study based on microscopic examination in drowned penguins.

Emerging and Re-emerging Diseases of Selected Avian Species.

Emerging infections and re-emerging diseases in birds can be caused by numerous factors and need to be recognized and understood. This article introduces and summarizes author-selected emerging and re-emerging diseases of avian species. These diseases hold significance as they relate to scientific research, disease recognition and identification, avian welfare aspects, and ecosystem health. Some are significant in human health and others affect production medicine. These and many others remain important pathogens of worldwide consequence.

Effects of Perching Surfaces and Foot Bandaging on Central Metatarsal Foot Pad Weight Loading of the Peregrine Falcon (Falco peregrinus).

Pododermatitis is prevalent in falcons and is characterized by inflammation and infection of the plantar aspect of the feet, particularly at the central metatarsal pad. Suboptimal perch design and increased weight loading on the plantar skin have been proposed as major risk factors for the development of pododermatitis. Prevention and treatment are challenging, but weight load reduction on the affected area of the foot is an accepted goal of initial treatment. To date, to our knowledge no studies have compared the performance of different bandage-perch surface combinations on weight redistribution away from the central metatarsal pad. An ex vivo factorial experiment was designed using the feet from a peregrine falcon cadaver to quantify weight load reduction on the central metatarsal pad with different combinations of perch surfaces (wood, neoprene, artificial turf) and bandages (none, interdigital, silicone shoe). Feet were attached to a digital force gauge mounted on a manual test stand for compression testing. Weight loading at the central metatarsal pad was measured using a small force sensor. Perch-surface combinations in randomized order were tested at 250 g, 500 g, and 1 kg with 9 replicates per foot. At 250 g, all combinations reduced measured metatarsal pad forces, but otherwise performed similarly. As compression forces increased, differences emerged with the shoe combinations performing better overall, followed by a group including the neoprene and artificial turf-interdigital bandage combinations, and a third group including the interdigital/wood and no bandage-artificial turf. All combinations performed better than control (no bandage on wood). This study may assist veterinarians in selecting appropriate perching surface/bandage combinations when treating falcons with pododermatitis.

Sterile traumatic panniculitis in a captive Brent goose.

A captive, adult female Brent goose (Branta bernicla) with a history of severe feather picking by its mate, was presented with 0.5-2.5 cm skin nodules on the head and neck. Histologic examination revealed a well-delineated dermal mass that surrounded an intact feather follicle and was composed of lakes of proteinaceous fluid and fibrin with scattered foamy macrophages and multinucleate giant cells. No bacteria or fungi were identified with histology, microbial culture, or PCR. Sterile panniculitis is an infrequent finding in animals and traumatic panniculitis is rarely sterile.

Pododermatitis in raptors admitted in a wildlife rehabilitation centre in central spain.

Pododermatitis is a chronic and progressive bacterial infectious disease of birds´ footpads, especially associated with captivity, which could represent an important problem in wildlife rehabilitation centres, delaying the release of the birds to the environment and harming their conservation. The objectives of the present study were i) to estimate the incidence rate of pododermatitis, ii) to analyse the influence of environmental, physiological, and pathological factors which may influence the onset of the infection in raptors after their admission to a rehabilitation centre, and iii) to follow up the evolution and macroscopic characteristics of the lesion. An observational retrospective study was carried out on clinical cases of raptors (n = 2004) admitted in a rehabilitation centre in central Spain from 2009 to 2015. The proportion of pododermatitis was 6.9%, with an incidence density of 6.8 cases/100 bird-years at risk. Our results showed that the disease was more likely to occur in birds staying longer (P < 0.001). According to Cox analysis, the variables 'raptor family' (P < 0.001) and 'age' (P = 0.002) may have significant effects on pododermatitis development. Raptors from the Falconidae and Strigidae families were less likely to develop pododermatitis than those from the Accipitridae family, with a hazard ratio (HR) of 0.31 and 0.13, respectively. Regarding age, fully feathered raptors had a three-fold higher risk of having pododermatitis than non-fully feathered birds. Survival analysis of time of pododermatitis development revealed significant differences associated with the variables 'raptor family' (P < 0.03); 'age' (P < 0.001) and 'cause of admission' (P = 0.001). Duration of the pathology (P < 0.05) and lesion location (P < 0.001) seemed to be phylogenetic dependent. Injuries were primarily located in the toes area or the whole footpad of birds belonging to the Accipitridae or Falconidae families, respectively (P = 0.003).

Clinical Management of Avian Renal Disease.

Treatment of avian renal disease relies on supportive care, such as fluid therapy and nutritional support. Analgesia and adaptations of the environment are indicated in cases of renal disease associated with painful joints. Other treatments vary with the underlying etiology and may include systemic antibiotics, antifungal therapy, vitamin A supplementation, or chelation therapy.

Mortality of four captive-born crested screamer chicks (Chauna torquata).

Crested screamers, a unique, mainly terrestrial avian species native to South America, are known to have a markedly high chick mortality rate in captivity, ranging from 61% to 94%; however, there is very limited information on this species' natural history within the literature, and even less about common diseases that affect them. Four captive-born crested screamer chicks (Chauna torquata) at a U.S. zoological institution died acutely from different causes over the course of 2.5 months. Although a hands-off approach was initially taken, each chick became acutely weak on exhibit and medical intervention was deemed necessary, but proved unsuccessful in all cases. Necropsy results of the chicks revealed various causes of death, including acute Escherichia coli colitis, aspiration pneumonia complicated by concurrent gastrointestinal Candidiasis, severe dehydration and emaciation, and acute amoebic gastroenteritis. No direct associations were found between these deaths and diet or husbandry; however, the limited literature on this topic suspects inadequate husbandry and immunosuppression to be the greatest cause of chick mortality in this species. The cases presented here are consistent with this hypothesis, but further exemplify the limited knowledge of this species and the need to optimize their survivability and proliferation in captivity.