Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens

This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and Microsporum gypseum) in vitro. Different concentrations (5, 10, 15 and 20 percent) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20 percent ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid Chromatography (HPLC) analysis of ethyl acetate extract showed the presence of a main component (nimonol) which was purified and chemically confirmed by Nuclear Magnetic Resonance (NMR) spectroscopic analysis. The 20 percent ethyl acetate extract lost a part of its antifungal effect after pooling out the nimonol and this loss in activity was variable on test pathogens. The purified nimonol as a separate compound did not show any antifungal activity when assayed against all the six fungal pathogens.

A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.

Divergência genética entre acessos de espinheira-santa (Maytenus ilicifolia Mart. ex Reissek e M. aquifolium Mart.) com base em caracteres morfológicos e fisiológicos / Genetic divergence among espinheira-santa (Maytenus ilicifolia Mart. ex Reiss. and M. aquifolium Mart.) accessions based on morphological and physiological traits

Maytenus ilicifolia e M. aquifolium (espinheira-santa) são espécies medicinais nativas do Brasil ameaçadas de extinção devido à forte ação antrópica nas populações naturais. O objetivo deste trabalho foi avaliar a divergência genética em germoplasma de espinheira-santa, e agrupar os acessos através de análises multivariadas, com base em caracteres morfo-fisiológicos das progênies. Foram avaliados 89 acessos do banco ativo de germoplasma de espinheira-santa da Embrapa Clima Temperado/Universidade Federal de Pelotas. Os caracteres avaliados foram dias da semeadura à emergência; altura e diâmetro à base do caule, aos 90, 180, 270 e 360 dias; crescimento em altura e diâmetro à base do caule; comprimento e largura de folha e número de espinhos por folha. O delineamento experimental foi em blocos casualizados, com quatro repetições e cinco plantas por parcela. Os dados foram submetidos à análise de variância e análise de variância multivariada, sendo estimadas as variáveis canônicas. Os caracteres avaliados foram eficientes para separar os acessos em três grupos. As variáveis altura, diâmetro à base do caule aos 360 dias e número de folhas aos 180 dias são as mais importantes para caracterização do germoplasma de espinheira-santa.

Maytenus ilicifolia and M. aquifolium (espinheira-santa) are medicinal species native to Brazil. They are endangered due to the strong anthropic action in natural populations. The aim of this work was to evaluate the genetic divergence in "espinheira-santa" germplasm and to cluster accessions by multivariate analyses based on morphophysiological traits of progenies. Eighty-nine accessions of the "espinheira-santa" active germplasm bank from the Brazilian Agricultural Research Corporation (EMBRAPA) Temperate Agriculture, Pelotas Federal University, Pelotas Municipality, Rio Grande do Sul State, Brazil, were evaluated regarding days from sowing to emergence; height and stem-base diameter at 90, 180, 270 and 360 days; height and stem-base diameter growth; leaf length and width; and number of thorns per leaf. Experimental design was in randomized blocks, with four replicates and five plants per plot. The results were subjected to analysis of variance and multivariate analysis of variance, and canonical variables were estimated. The evaluated traits were efficient for separating the accessions into three clusters. Height and stem-basal diameter at 360 days and leaf number at 180 days were the most important for "espinheira-santa" germplasm characterization.

New hosts of Myrothecium spp. in Brazil and a preliminary in vitro assay of fungicides

Myrothecium roridum and M. verrucaria are two plant pathogenic species causing foliar spots in a large number of cultivated plants. This paper aims to study the causal agents of foliar spots in vegetable crops (sweet pepper, tomato and cucumber), ornamental plants (Spathiphyllum wallisii, Solidago canadensis, Anthurium andreanum, Dieffenbachia amoena) and a solanaceous weed plant (Nicandra physaloides). Most of the isolates were identified as M. roridum; only the isolate 'Myr-02' from S. canadensis was identified as M. verrucaria. All the isolates were pathogenic to their original plant hosts and also to some other plants. Some fungicides were tested in vitro against an isolate of M. roridum and the mycelial growth recorded after seven days. Fungicides with quartenary ammonium, tebuconazole and copper were highly effective in inhibiting the mycelial growth of M. roridum. This paper confirms the first record of M. roridum causing leaf spots in sweet pepper, tomato, Spathiphyllum, Anthurium, Dieffenbachia and N. physaloides in Brazil. We also report M. roridum as causal agent of cucumber fruit rot and M. verrucaria as a pathogen of tango plants.