RESUMEN
BACKGROUND: As the primary decision-maker for their horse's health and welfare, owners' knowledge of clinical conditions may impact their horse's health. Anecdotal evidence suggests that the emotional impact of equine illness on the owner can be severe but research is lacking. OBJECTIVES: To evaluate horse owners' self-declared knowledge of eight common equine health conditions and perceptions of the quality of information available; to ascertain respondents' perceptions of the severity of impact on their horse of the conditions and potential emotional impact on themselves and to establish the factors of greatest concern to owners when their horse has a health condition and influential factors on end-of-life decisions. STUDY DESIGN: Online survey of horse owners. METHODS: An online survey comprising three sections was created: respondent demographics; vignettes of clinical conditions; concerns and decision-making regarding illness and euthanasia. Vignettes focused on arthritis; Pars Pituitary Intermedia Dysfunction; Equine Metabolic Syndrome (EMS); gastric ulcers; high intestinal worm burden; dermatophilosis ('mud rash'); quidding and head tossing; and equine asthma. Respondents were self-selecting. The survey was circulated for completion by horse owners residing in Ireland and Northern Ireland obtaining 491 valid responses. RESULTS: Respondents declared that they had the highest knowledge of mud rash, with 84.1% rating their knowledge as very good compared to 42% of respondents for EMS. There was positive correlation between the perceived impact on their horse and emotional impact on the owner for all conditions ranging from weak (arthritis 0.36, P < 0.001) to strong (EMS 0.62, P < 0.001). Pain, discomfort, and quality of life were reported as the most common concerns when their horse has a medical condition or when considering euthanasia. MAIN LIMITATIONS: Potential bias among respondents towards computer literate owners, with good literacy and interest in equine health conditions. CONCLUSIONS: Knowledge of equine health conditions, perceived impact of conditions on the horse and emotional impact on their owners varied significantly between conditions.
Asunto(s)
Artritis , Exantema , Enfermedades de los Caballos , Síndrome Metabólico , Caballos , Animales , Calidad de Vida , Enfermedades de los Caballos/epidemiología , Encuestas y Cuestionarios , Exantema/veterinaria , Síndrome Metabólico/veterinaria , Artritis/veterinariaRESUMEN
Equid alphaherpesvirus 3 (EHV-3) is the etiological agent of equine coital exanthema (ECE). Because no vaccines or antiviral therapies are available, prevention consists of clinical examination of mares and stallions before mating or semen collection and resting from breeding activities when lesions are present. However, this methodology does not identify subclinically infected animals. Ganciclovir is the most potent compound known to reduce EHV-3 replication. This study aimed to evaluate the efficacy of topical ganciclovir application to reduce EHV-3 replication in experimentally infected mares. A pilot study, after a double-blind completely randomized design, was carried out. Twenty mares were randomly divided into five groups (three treated with ganciclovir with different regimen of doses, one treated with a placebo, and one nontreated). Mares were experimentally infected with EHV-3 on day 0. Rectal temperature, clinical signs, and lesions were recorded. Daily perineal and vaginal swabs were evaluated by quantitative polymerase chain reaction for virus detection. The antibody response was assessed by a virus neutralization test in serum samples collected weekly. Mares experimentally infected with EHV-3 and treated with ganciclovir twice a day for 13 days showed reduced levels and duration of viral excretion and less severe lesions. The viral excretion period was reduced from 18 to nine days compared with the untreated groups. We concluded that ganciclovir had an antiviral effect on EHV-3 replication when topically administered in mares showing clinical signs of ECE. Further trials should be performed to optimize the dose of the antiviral for a definitive formulation.
Asunto(s)
Exantema , Herpesvirus Équido 3 , Enfermedades de los Caballos , Animales , Exantema/veterinaria , Femenino , Ganciclovir , Caballos , Masculino , Proyectos PilotoRESUMEN
In 2017, two Thoroughbred stallions, A and B in Farms A and B, respectively, in Hokkaido in Japan showed clinical signs of equine coital exanthema (ECE). In 2020, stallion C in Farm B showed clinical signs of ECE. Eighteen mares were mated within five days before stallion A developed ECE. Ten mares that mated within 3 days before onset showed clinical signs of ECE on the external genitalia. Equine herpesvirus 3 (EHV-3) was isolated from vaginal swabs from three mares that mated within 2 days before onset. Swabs from 12 mares that mated within 4 days before onset were real-time PCR (rPCR)-positive and nine of those mares had an increased EHV-3 antibody titer. The three stallions were administered valaciclovir orally and topical acyclovir ointment was applied. Treatment started on the next day after onset in stallion A and on the day of onset in stallions B and C. EHV-3 was firstly isolated from penis swabs of stallions A and B before treatment and from penis swabs of stallion C 2 days after treatment. EHV-3 was not isolated after 8, 5 and 8 days from onset in stallions A, B and C, respectively. However, swabs were rPCR-positive for at least 12, 9 and 15 days after onset of stallions A, B and C, respectively. EHV-3 was excreted from the stallions at least within 4 days before the onset of ECE, and acyclovir treatment resulted in the termination of excretion within 8 days after onset.
Asunto(s)
Exantema , Infecciones por Herpesviridae , Herpesvirus Équido 1 , Herpesvirus Équido 3 , Enfermedades de los Caballos , Aciclovir/uso terapéutico , Animales , Exantema/veterinaria , Femenino , Infecciones por Herpesviridae/tratamiento farmacológico , Infecciones por Herpesviridae/veterinaria , Enfermedades de los Caballos/tratamiento farmacológico , Caballos , Japón , MasculinoRESUMEN
Equine coital exanthema (ECE) has been reported in many countries, but equine herpesvirus 3 (EHV-3) has been isolated only once in Japan. In 2015, symptoms of ECE were found, and EHV-3 was isolated in two stallions. Valacyclovir, an anti-herpesvirus agent, was administered orally. The stallions rested from mating for more than two weeks, causing enormous financial losses because of their high fees. This is the first study in which valacyclovir was administered for ECE. Though valacyclovir treatment did not shorten the duration of healing, the affected area did not expand after administration of valacyclovir. Valacyclovir therefore seems to be effective for suppression of EHV-3 infection. Further investigation about the administration protocol might be required.
Asunto(s)
Aciclovir/análogos & derivados , Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Herpesvirus Équido 3/aislamiento & purificación , Enfermedades de los Caballos/epidemiología , Enfermedades Virales de Transmisión Sexual/veterinaria , Valina/análogos & derivados , Aciclovir/uso terapéutico , Animales , Antivirales/uso terapéutico , Exantema/tratamiento farmacológico , Exantema/epidemiología , Exantema/virología , Infecciones por Herpesviridae/tratamiento farmacológico , Infecciones por Herpesviridae/epidemiología , Enfermedades de los Caballos/tratamiento farmacológico , Enfermedades de los Caballos/virología , Caballos , Japón , Masculino , Enfermedades Virales de Transmisión Sexual/tratamiento farmacológico , Enfermedades Virales de Transmisión Sexual/epidemiología , Valaciclovir , Valina/uso terapéuticoRESUMEN
In the spring of 2015, two stallions reared in Farms A and B in Hokkaido in Japan showed symptoms of equine coital exanthema. Equine herpesvirus 3 (EHV-3) was isolated from penis swab samples of both stallions, and the isolates from each stallion in Farms A and B were designated as SS-1 and YS-1 strains, respectively. BamHI restriction profiles of SS-1 and Japanese reference strain Iwate-1 were indistinguishable, but the BamHI-A fragment of YS-1 was larger than those of SS-1 and Iwate-1 by 1.9 kbp because of the lack of two BamHI sites. Nucleotide sequence analyses of glycoprotein G (gG), gB, gC and VP13/14 coding regions revealed that SS-1 and YS-1 had 99.77% to 100% identities to each other. These results suggested that the origins of SS-1 and YS-1 were different. For a sero-epidemiological survey, serum neutralizing tests using SS-1 against 319 sera of horses from eight farms in Hokkaido were conducted. Six of the eight farms were EHV-3 antibody-positive, and positive rates ranged from 2.6% to 17.6%. To determine the infection time of four EHV-3 antibody-positive horses, a retrospective study was conducted. Infection time of the four horses was in the breeding season, and re-infection or reactivation of latently infected EHV-3 might have occurred in one horse. However, these four horses had never shown any clinical symptoms. The results suggested that several EHV-3 strains are distributed in Japan and that infection is maintained widely in horses without clinical symptoms.
Asunto(s)
Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Herpesvirus Équido 3/aislamiento & purificación , Enfermedades de los Caballos/virología , Enfermedades Virales de Transmisión Sexual/veterinaria , Animales , Exantema/epidemiología , Exantema/virología , Infecciones por Herpesviridae/epidemiología , Infecciones por Herpesviridae/virología , Enfermedades de los Caballos/epidemiología , Caballos , Japón , Masculino , Análisis de Secuencia de ADN , Estudios Seroepidemiológicos , Enfermedades Virales de Transmisión Sexual/epidemiología , Enfermedades Virales de Transmisión Sexual/virologíaRESUMEN
REASONS FOR PERFORMING STUDY: In August 2014, an outbreak of oral exanthematous disease in equids was reported in Brazil, affecting 11 donkeys and 3 mules. OBJECTIVES: To investigate if Vaccinia virus (VACV) was the aetiological agent in this outbreak. STUDY DESIGN: Investigation of clinical cases using serological, molecular and phylogenetic approaches. METHODS: To analyse the presence of neutralising antibodies against VACV, samples were submitted in triplicate to a plaque-reduction neutralisation test (PRNT50% ). On the basis of previous studies which detected VACV DNA in sera, we submitted extracted DNA samples to different polymerase chain reaction (PCR) platforms targeting Orthopoxvirus (OPV) genes (C11R, A56R and A26L). The PCR products were directly sequenced in both orientations using specific primers and capillary electrophoresis. The alignment and phylogenetic analysis of the A26L and A56R nucleotide sequences (maximum likelihood) were prepared with the obtained nucleotide fragments. RESULTS: Serological and molecular data suggested VACV as the aetiological agent. The neutralising antibodies against OPV were detected in 5 (55.5%) of the equids, with titres ≥40 neutralising u/ml. Based on the results obtained from all PCR platforms, all samples were positive for OPV: 9 (100%) for A56R, 4 (44.4%) for C11R and 3 (33.3%) for A26L. The alignment of the nucleotide sequences of the A26L and A56R fragments revealed that the samples were highly similar to the homologous genes from other Brazilian VACV Group 1 isolates (98.8% identity on average). Furthermore, both the A26L and A56R sequences showed signature deletions also present in the sequences of Group 1 VACV isolates from Brazil. CONCLUSIONS: Our data raises questions about the role of equids in the chain of VACV epidemiology. The surveillance of equids in VACV-affected areas worldwide is relevant.
Asunto(s)
Brotes de Enfermedades/veterinaria , Equidae , Exantema/veterinaria , Enfermedades de la Boca/veterinaria , Virus Vaccinia/aislamiento & purificación , Vaccinia/veterinaria , Animales , Brasil/epidemiología , Exantema/patología , Exantema/virología , Enfermedades de la Boca/epidemiología , Enfermedades de la Boca/patología , Enfermedades de la Boca/virología , Vaccinia/diagnóstico , Vaccinia/epidemiología , Vaccinia/patologíaRESUMEN
Vaccinia virus (VACV) is responsible for outbreaks in Brazil and has immense potential as an emerging virus. VACV can be found naturally circulating in India, Pakistan and South America, where it causes infections characterised by exanthematic lesions in buffaloes, cattle and humans. The transmission cycle of Brazilian VACV has still not been fully characterised; one of the most important gaps in knowledge being the role of wild animals. Capybaras, which are restricted to the Americas, are the world's largest rodents and have peculiar characteristics that make them possible candidates for being part of a natural VACV reservoir. Here, we developed a method for detecting orthopoxvirus DNA in capybara stool samples, and have described for the first time the detection of orthopoxvirus DNA in capybaras samples from three different regions in Brazil. These findings strongly suggest that capybaras might be involved in the natural transmission cycle of VACV and furthermore represent a public health problem, when associated with Brazilian bovine vaccinia outbreaks. This makes infected animals an important factor to be considered when predicting and managing Brazilian VACV outbreaks.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , ADN Viral/genética , Brotes de Enfermedades/veterinaria , Exantema/veterinaria , Enfermedades de los Roedores/epidemiología , Roedores/virología , Virus Vaccinia/genética , Secuencia de Aminoácidos , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/transmisión , Enfermedades de los Bovinos/virología , ADN Viral/aislamiento & purificación , Exantema/epidemiología , Exantema/virología , Heces/virología , Femenino , Masculino , Enfermedades de los Roedores/transmisión , Enfermedades de los Roedores/virología , Virus Vaccinia/aislamiento & purificaciónRESUMEN
Equine coital exanthema (ECE) caused by equid herpesvirus 3 (EHV-3) is a contagious venereal disease characterised by the formation of painful papules, vesicles, pustules and ulcers on the external genitalia of both mares and stallions. EHV-3 is an alphaherpesvirus that is distinct from the other equine herpesviruses and endemic in most horse breeding populations worldwide. The negative impacts of ECE on equine breeding enterprises are the forced, temporary disruption of mating activities of mares and stallions, the additional care and supportive treatment that is required for affected horses, and the risk of virus spread by either fresh or frozen semen as well as by artificial insemination and embryo transfer. Because there are no effective surveillance systems to report ECE, its true prevalence and economic impact are difficult to assess and are probably underestimated. The purpose of this review is to describe the recent advances in understanding of EHV-3 infections and to consider the economic consequences of ECE within the current context of the equine industry.
Asunto(s)
Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Herpesvirus Équido 3/aislamiento & purificación , Enfermedades de Transmisión Sexual/veterinaria , Animales , Bélgica , Cruzamiento , Transmisión de Enfermedad Infecciosa/veterinaria , Transferencia de Embrión/veterinaria , Exantema/prevención & control , Femenino , Genitales/virología , Infecciones por Herpesviridae/prevención & control , Caballos , Industrias , Inseminación Artificial/veterinaria , Masculino , Enfermedades de Transmisión Sexual/prevención & controlRESUMEN
Ticks are hematophagous parasites of people and animals and are a public health hazard in several countries. They are vectors of infectious diseases; in addition, the bite of some ticks, mainly from the Ornithodoros genus, may lead to local lesions and systemic illness, referred to as tick toxicosis. In this report, we describe a dog bitten by Ornithodoros brasiliensis, popularly known as the mouro tick. The main clinical findings were disseminated skin rash, pruritus, mucosal hyperemia, lethargy, and fever. Laboratory abnormalities 48 hours after the bites occurred included mild nonregenerative anemia, eosinophilia, basophilia, increased serum creatine kinase activity, increased serum C-reactive protein concentration, and prolonged coagulation times. Tick-borne pathogens were not detected by PCR analysis or serologic testing, supporting the diagnosis of a noninfectious syndrome due to tick bite, compatible with tick toxicosis.
Asunto(s)
Mordeduras y Picaduras/veterinaria , Enfermedades de los Perros/diagnóstico , Ornithodoros/fisiología , Infestaciones por Garrapatas/veterinaria , Toxicosis por Garrapatas/veterinaria , Animales , Vectores Arácnidos/clasificación , Vectores Arácnidos/fisiología , Mordeduras y Picaduras/complicaciones , Brasil , Proteína C-Reactiva/metabolismo , Diagnóstico Diferencial , Enfermedades de los Perros/parasitología , Perros , Exantema/parasitología , Exantema/veterinaria , Fiebre/parasitología , Fiebre/veterinaria , Hiperemia/parasitología , Hiperemia/veterinaria , Letargia/parasitología , Letargia/veterinaria , Masculino , Ornithodoros/clasificación , Prurito/parasitología , Prurito/veterinaria , Infestaciones por Garrapatas/complicaciones , Infestaciones por Garrapatas/parasitología , Toxicosis por Garrapatas/diagnóstico , Toxicosis por Garrapatas/parasitologíaRESUMEN
During the last bovine vaccinia (BV) outbreaks, several Vaccinia virus (VACV) strains were isolated and characterised, revealing significant polymorphisms between strains, even within conserved genes. Although the epidemiology of VACV has been studied in BV outbreaks, there is little data about the circulation of the Brazilian VACV isolates. This study describes the genetic and biological characterisation of two VACV isolates, Pelotas 1 virus (P1V) and Pelotas 2 virus (P2V), which were obtained concomitantly from a horse affected by severe cutaneous disease. Despite being isolated from the same exanthematic clinical sample, P1V and P2V showed differences in their plaque phenotype and in one-step growth curves. Moreover, P1V and P2V presented distinct virulence profiles in a BALB/c mouse model, as observed with other Brazilian VACV isolates. Sequencing and phylogenetic analysis of four different genes demonstrated that the isolates are segregated in different VACV clusters. Our results raise interesting questions about the diversity of VACV isolates in Brazil.
Asunto(s)
Exantema/veterinaria , Enfermedades de los Caballos/virología , Virus Vaccinia/genética , Vaccinia/veterinaria , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Brasil , Bovinos , ADN Viral/genética , Exantema/virología , Genes Virales , Hemaglutininas Virales/genética , Caballos , Masculino , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Filogenia , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido Nucleico , Especificidad de la Especie , Vaccinia/virología , Virus Vaccinia/clasificación , Virus Vaccinia/aislamiento & purificación , Virus Vaccinia/patogenicidad , Virulencia/genéticaRESUMEN
During the period from 2001 to the following year, progenital diseases had been epidemic among the draft stallions and mares pastured together in Iwate Prefecture, the northeastern district of Japan. A stallion and 8 of 31 mares were affected in 2001, and 1 of 2 stallions and 10 of 36 mares in 2002. The clinical symptoms consisted of the formation of papules, pustules, ulcers and scabs on the progenital skin and mucosa in stallions and mares. In 2002, Equine herpesvirus 3 (EHV3) was isolated from 2 mares and the glycoprotein G gene of the virus detected from a stallion and 4 mares by polymerase chain reaction. Serum neutralizing tests showed that 12 of 38 horses, 10 clinically and 2 subclinically affected, changed to be positive for the EHV3 antibody. The results suggest that the horses were affected with equine coital exanthema (ECE) through coitus. Five mares with the antibody at the pre-pastured period may have been the possible origins of EHV3 infection in 2002, although the exact origin in 2001 remains unknown. The artificial insemination was performed for the prevention of ECE spreading through coitus on the pasture in 2003. There was no epidemic of the disease in 31 mares, although 3 mares with the antibody at the pre-pastured period showed the significant increase in the titers during the pastured period.
Asunto(s)
Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Herpesvirus Équido 3 , Enfermedades de los Caballos/virología , Enfermedades Virales de Transmisión Sexual/veterinaria , Animales , Secuencia de Bases , Cartilla de ADN , Electroforesis en Gel de Agar/veterinaria , Exantema/patología , Exantema/virología , Genitales Masculinos/patología , Genitales Masculinos/virología , Infecciones por Herpesviridae/epidemiología , Infecciones por Herpesviridae/patología , Enfermedades de los Caballos/epidemiología , Enfermedades de los Caballos/patología , Caballos , Japón/epidemiología , Masculino , Datos de Secuencia Molecular , Pruebas de Neutralización/veterinaria , Reacción en Cadena de la Polimerasa/veterinaria , Alineación de Secuencia , Análisis de Secuencia de ADN/veterinaria , Enfermedades Virales de Transmisión Sexual/epidemiología , Proteínas del Envoltorio Viral/metabolismoRESUMEN
Two 5-year-old grade male horses presented with epiphora, rhinorrhea, conjunctival and nasal mucosal hyperemia, and dorsal and thoracic macropapular rash. Skin biopsies were collected from the affected areas, and serial sections were evaluated following hematoxylin and eosin and immunoperoxidase histochemistry staining by using a murine monoclonal antibody of the immunoglobulin G2A isotype recognizing the 30-kDa membrane protein of equine arteritis virus (EAV). In both horses, lesions consisted of mild to moderate diffuse superficial dermal edema and vasculitis with mild perivascular lymphocytic infiltrates, occasional endothelial hypertrophy, and single-cell necrosis of tunica media myocytes. Immunohistochemically, a few endothelial cells, myocytes, and pericytes containing intracytoplasmic EAV antigen were identified. Immunoperoxidase histochemistry of skin biopsies can be used as an ancillary test for the clinical diagnosis of equine viral arteritis in horses, especially when a cutaneous macropapular rash is evident.
Asunto(s)
Infecciones por Arterivirus/veterinaria , Equartevirus , Enfermedades de los Caballos/diagnóstico , Animales , Anticuerpos Monoclonales , Infecciones por Arterivirus/complicaciones , Infecciones por Arterivirus/diagnóstico , Infecciones por Arterivirus/patología , Biopsia/veterinaria , Exantema/complicaciones , Exantema/patología , Exantema/veterinaria , Enfermedades de los Caballos/patología , Enfermedades de los Caballos/virología , Caballos , Técnicas para Inmunoenzimas/veterinaria , Enfermedades del Aparato Lagrimal/complicaciones , Enfermedades del Aparato Lagrimal/veterinaria , Masculino , Mucosa Nasal/metabolismo , Piel/irrigación sanguínea , Piel/patologíaAsunto(s)
Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Herpesvirus Cercopitecino 1 , Hígado/patología , Macaca fascicularis , Enfermedades de los Monos/diagnóstico , Úlceras Bucales/veterinaria , Animales , Exantema/virología , Infecciones por Herpesviridae/diagnóstico , Leucocitosis/veterinaria , Masculino , Enfermedades de los Monos/virología , Necrosis , Neutrófilos , Úlceras Bucales/virología , Trombocitopenia/veterinaria , Trombocitopenia/virología , ViremiaRESUMEN
A progenital disease encountered at one equine stud farm at Bangalore in Southern India during 1987 was investigated and confirmed as equine coital exanthema on the basis of characteristic lesions and clinical symptoms, isolation of equine herpes virus-3 (EHV-3) from the scabs collected from animals having active lesions and demonstration of neutralizing antibodies in the sera of recovered mares and stallion. This is the first authenticated report of the occurrence of equine coital exanthema in India due to EHV-3.
Asunto(s)
Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Enfermedades de los Caballos/diagnóstico , Animales , Embrión de Pollo , Exantema/diagnóstico , Femenino , Infecciones por Herpesviridae/diagnóstico , Caballos , India , MasculinoRESUMEN
Equine coital exanthema can be produced experimentally in stallions by inoculation with an equine herpesvirus (strain 65/61) and be transmitted during coitus with an infected mare. Serological responses to this infection include the production of complement-fixing and serum-neutralizing antibodies which reach maximum levels 14 to 21 days after infection. Complement-fixing antibodies decline rapidly and are usually not detectable by 60 days after infection, whereas serum-neutralizing antibody activity is maintained for at least 1 year. This disparity provides a useful method for the diagnosis of recent infections and estimation of the temporal incidence of infection in groups of mares. It seems likely that clinically normal carrier mares spread the virus on studs where the disease has previously not occurred.
Asunto(s)
Exantema/veterinaria , Infecciones por Herpesviridae/veterinaria , Enfermedades de los Caballos/etiología , Enfermedades de Transmisión Sexual/veterinaria , Animales , Australia , Exantema/epidemiología , Exantema/etiología , Femenino , Fertilidad , Infecciones por Herpesviridae/epidemiología , Herpesvirus Équido 3 , Enfermedades de los Caballos/epidemiología , Caballos , Masculino , Enfermedades de Transmisión Sexual/epidemiología , Enfermedades de Transmisión Sexual/etiologíaRESUMEN
The infectious bovine rhinotracheitis (IBR) virus belongs to the group of herpes viruses. It causes in cattle a severe disease predominantly in the upper respiratory tract. Morbidity rate is 100 percent, mortality - depending on hygienic and other factors - ranges from 0-15 percent. The IBR virus is serologically indistinguishable from the infect pustulat vulvovaginitis (IPV) virus which causes disorders of the genital tract in both male and female cattle. Possibly the IPV virus is the older of the two. Already in the twenties it had been isolated and described in Europe whereas IBR virus was first isolated from feedlot cattle in the USA during 1955/1956. Most likely - experiments also gave a hint - the IPV virus increased its virulence for the respiratory tract by rapid and numerous passages as this was only possible in accumulations of tremendous heads of animals. Vaccines both live attenuated and killed ones have been developed. Killed ones are always administered parenterally; the live ones are either intramuscularly injected or locally sprayed (into the nasal cavity). Immunity is developed within 3 weeks, however, if vaccinated animals are challenged, virus is again shed for a number of days. Cattle thereby do not exhibit any symptoms of disease. This is true following any kind of vaccination (live or killed). After local administration of live vaccines, however, the production of interferon by the cells of the respiratory tract is remarkable whereas parenterally administration of vaccine does not lead to that.