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1.
J Ethnopharmacol ; 270: 113785, 2021 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-33422653

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: The Scutellariae Radix (SR) and Coptidis Rhizoma (CR) herb couple is widely used in traditional Chinese medicine prescriptions for the treatment of diabetes mellitus due to its interaction and synergistic effect compared to either herb alone, but the underlying mechanism of interaction between these herbs is unclear. This study aimed to investigate the effects of CR on the metabolism and absorption of SR. MATERIALS AND METHODS: After rats were treated with normal saline (NS group) or the CR extract (CR-treated group) for seven consecutive days, the intestinal flora was extracted from rat faeces for a co-incubation with the SR extract to investigate the metabolism of SR flavonoids, and a non-everted gut sac was prepared in vitro to evaluate the intestinal absorption of the SR extract. The components of the SR extract, the metabolites of the SR extract that was co-incubated with intestinal flora, and the dialysate acquired from non-everted gut sacs were identified and determined by an HPLC-MS/MS method. The absorption rate constant (Ka) and the apparent permeability (Papp) of each compound were calculated, and the effects of CR on the metabolism and absorption of flavonoids in SR were evaluated, by comparison the Ka and Papp between two groups using Student's t-test. RESULTS: Twenty-nine flavonoids were detected and identified in the SR extract, including 16 glycosides and 13 aglycones. In the co-incubation with the intestinal flora, differences in metabolite classes were not observed between the NS group and CR-treated group; however, the metabolic rates of 17 flavonoids in the CR-treated group were significantly higher than the NS group. The Papp of 11 compounds (4 glycosides and 7 aglycones) across the gut sac were greater than 2 × 10-5 cm/s in both groups, while the Papp values of 7 compounds including wogonoside (WG) and other aglycones were significantly decreased in the CR-treated group. CONCLUSION: Based on these results, CR decreased the metabolism and absorption of SR flavonoids, and exerted much greater inhibitory effects on aglycones than glycosides, which may be one of the potential mechanisms underlying the therapeutic effects of the combination of SR and CR on diabetes mellitus.


Asunto(s)
Medicamentos Herbarios Chinos/farmacología , Absorción Intestinal/efectos de los fármacos , Extractos Vegetales/metabolismo , Extractos Vegetales/farmacocinética , Animales , Cromatografía Líquida de Alta Presión/métodos , Coptis chinensis , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/química , Heces/química , Flavonoides/antagonistas & inhibidores , Flavonoides/metabolismo , Flavonoides/farmacocinética , Contenido Digestivo/química , Microbioma Gastrointestinal/efectos de los fármacos , Glicósidos/antagonistas & inhibidores , Glicósidos/metabolismo , Glicósidos/farmacocinética , Masculino , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Ratas Sprague-Dawley , Scutellaria baicalensis , Espectrometría de Masas en Tándem/métodos
2.
Clin Toxicol (Phila) ; 56(4): 285-293, 2018 04.
Artículo en Inglés | MEDLINE | ID: mdl-28871819

RESUMEN

CONTEXT: Antidote shortage is a global problem. In Thailand, the National Antidote Project (NAP) has operated since November 2010 to manage the national antidote stockpile, educate the healthcare providers on appropriate antidote use, and evaluate antidote usage. OBJECTIVE: To evaluate the effect of NAP implementation on mortality rate and antidote use in cyanide poisoning cases arising from ingestion of cyanide or cyanogenic glycoside. METHODS: This is a retrospective cohort of poisoning cases involving cyanide or cyanogenic glycoside ingestion reported to Ramathibodi Poison Center from 1 January 2007 to 31 December 2015. Mortality rate, antidote use, and appropriateness of antidote use (defined as correct indication, proper dosing regimen, and administration within 90 min) before and after NAP implementation were compared. Association between parameters and fatal outcomes was analyzed. RESULTS: A total of 343 cases involving cyanide or cyanogenic glycoside ingestion were reported to Ramathibodi Poison Center. There were 213 cases (62.1%) during NAP (Project group) and 130 cases (37.9%) pre-NAP implementation (Before group). Implementation of NAP led to increased antidote use (39.9% in Project group versus 24.6% in Before group) and a higher rate of appropriate antidote use (74.1% in Project group versus 50.0% in Before group). All 30 deaths were presented with initial severe symptoms. Cyanide chemical source and self-harm intent were associated with death (OR: 12.919, 95% CI: 4.863-39.761 and OR: 10.747, 95% CI: 3.884-28.514, respectively). No difference in overall mortality rate (13 [10.0%] deaths before versus 17 [8.0%] deaths after NAP) was found. In subgroup analysis of 80 cases with initial severe symptoms, NAP and appropriate antidote use reduced mortality (OR: 0.327, 95% CI: 0.106-0.997 and OR: 0.024, 95% CI: 0.004-0.122, respectively). In the multivariate analysis of the cases with initial severe symptoms, presence of the NAP and appropriate antidote use independently reduced the risk of death (OR: 0.122, 95% CI: 0.023-0.633 and OR: 0.034, 95% CI: 0.007-0.167, respectively), adjusted for intent of exposure, cyanide source, age, and sex. CONCLUSIONS: After NAP implementation, both antidote use and appropriate antidote use increased. In cases presenting with severe symptoms, presence of the NAP and appropriate antidote use independently reduced the risk of mortality.


Asunto(s)
Cianuros/envenenamiento , Centros de Control de Intoxicaciones , Adolescente , Adulto , Antídotos/uso terapéutico , Niño , Preescolar , Cianuros/antagonistas & inhibidores , Femenino , Glicósidos/antagonistas & inhibidores , Glicósidos/envenenamiento , Humanos , Masculino , Estudios Retrospectivos , Tailandia/epidemiología , Adulto Joven
3.
Planta Med ; 83(3-04): 261-267, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27574895

RESUMEN

Peltatoside is a natural compound isolated from leaves of Annona crassiflora Mart., a plant widely used in folk medicine. This substance is an analogue of quercetin, a flavonoid extensively studied because of its diverse biological activities, including analgesic effects. Besides, a previous study suggested, by computer structure analyses, a possible quercetin-CB1 cannabinoid receptor interaction. Thus, the aim of this work was to assess the antinociceptive effect of peltatoside and analyze the cannabinoid system involvement in this action. The mouse paw pressure test was used and hyperalgesia was induced by intraplantar injection of carrageenan (200 µg/paw). All used drugs were administered by intraplantar administration in Swiss male mice (n = 6). Peltatoside (100 µg/paw) elicited a local inhibition of hyperalgesia. The peripheral antinociceptive action of peltatoside was antagonized by the CB1 cannabinoid antagonist AM251 (160 µg/paw), but not by CB2 cannabinoid antagonist AM630 (100 µg/paw). In order to assess the role of endocannabinoids in this peripheral antinociceptive effect, we used (i) [5Z,8Z,11Z,14Z]-5,8,11,14-eicosatetraenyl-methyl ester phosphonofluoridic acid, an inhibitor of anandamide amidase; (ii) JZL184, an inhibitor for monoacylglycerol lipase, the primary enzyme responsible for degrading the endocannabinoid 2-arachidonoylglycerol; and (iii) VDM11, an endocannabinoid reuptake inhibitor. MAFP, JZL184, and VDM11 did not induce antinociception, respectively, at the doses 0.5, 3.8, and 2.5 µg/paw, however, these three drugs were able to potentiate the peripheral antinociceptive effect of peltatoside at an intermediary dose (50 µg/paw). Our results suggest that this natural substance is capable of inducing analgesia through the activation of peripheral CB1 receptors, involving endocannabinoids in this process.


Asunto(s)
Analgésicos/farmacología , Cannabinoides/metabolismo , Glicósidos/farmacología , Quercetina/análogos & derivados , Amidohidrolasas/metabolismo , Analgésicos/química , Analgésicos/aislamiento & purificación , Animales , Annona/química , Benzodioxoles/administración & dosificación , Benzodioxoles/farmacología , Antagonistas de Receptores de Cannabinoides/farmacología , Carragenina/antagonistas & inhibidores , Carragenina/farmacología , Relación Dosis-Respuesta a Droga , Endocannabinoides/metabolismo , Glicósidos/antagonistas & inhibidores , Glicósidos/química , Glicósidos/aislamiento & purificación , Hiperalgesia/tratamiento farmacológico , Masculino , Ratones , Monoacilglicerol Lipasas/efectos de los fármacos , Dimensión del Dolor/efectos de los fármacos , Piperidinas/administración & dosificación , Piperidinas/farmacología , Extractos Vegetales/farmacología , Pirazoles/farmacología , Quercetina/antagonistas & inhibidores , Quercetina/química , Quercetina/aislamiento & purificación , Quercetina/farmacología , Receptor Cannabinoide CB1/antagonistas & inhibidores , Receptor Cannabinoide CB1/metabolismo , Receptor Cannabinoide CB2/antagonistas & inhibidores , Receptor Cannabinoide CB2/metabolismo
4.
Eur J Pharmacol ; 767: 183-92, 2015 Nov 15.
Artículo en Inglés | MEDLINE | ID: mdl-26494631

RESUMEN

Peripheral painful neuropathy is one of the most common complications in diabetes and necessitates improved treatment. Secoisolariciresinol diglycoside (SDG), a predominant lignan in flaxseed, has been shown in our previous studies to exert antidepressant-like effect. As antidepressant drugs are clinically used to treat chronic neuropathic pain, this work aimed to investigate the potential analgesic efficacy of SDG against diabetic neuropathic pain in a mouse model of type 1 diabetes. We subjected mice to diabetes by a single intraperitoneal (i.p.) injection of streptozotocin (STZ, 200 mg/kg), and Hargreaves test or von Frey test was used to assess thermal hyperalgesia or mechanical allodynia, respectively. Chronic instead of acute SDG treatment (3, 10 or 30 mg/kg, p.o., twice per day for three weeks) ameliorated thermal hyperalgesia and mechanical allodynia in diabetic mice, and these analgesic actions persisted about three days when SDG treatment was terminated. Although chronic treatment of SDG to diabetic mice did not impact on the symptom of hyperglycemia, it greatly attenuated excessive oxidative stress in sciatic nerve and spinal cord tissues, and partially counteracted the condition of weight decrease. Furthermore, the analgesic actions of SDG were abolished by co-treatment with the reactive oxygen species donor tert-butyl hydroperoxide (t-BOOH), but potentiated by the reactive oxygen species scavenger phenyl-N-tert-butylnitrone (PBN). These findings indicate that chronic SDG treatment can correct neuropathic hyperalgesia and allodynia in mice with type 1 diabetes. Mechanistically, the analgesic actions of SDG in diabetic mice may be associated with its antioxidant activity.


Asunto(s)
Analgésicos/uso terapéutico , Antioxidantes/metabolismo , Butileno Glicoles/uso terapéutico , Diabetes Mellitus Tipo 1/complicaciones , Neuropatías Diabéticas/tratamiento farmacológico , Lino/química , Lignanos/uso terapéutico , Analgésicos/farmacología , Animales , Butileno Glicoles/antagonistas & inhibidores , Butileno Glicoles/farmacología , Óxidos N-Cíclicos/farmacología , Diabetes Mellitus Tipo 1/tratamiento farmacológico , Diabetes Mellitus Tipo 1/metabolismo , Neuropatías Diabéticas/inducido químicamente , Neuropatías Diabéticas/complicaciones , Neuropatías Diabéticas/metabolismo , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Sinergismo Farmacológico , Glicósidos/antagonistas & inhibidores , Glicósidos/farmacología , Glicósidos/uso terapéutico , Hiperalgesia/complicaciones , Hiperalgesia/tratamiento farmacológico , Hiperalgesia/metabolismo , Lignanos/antagonistas & inhibidores , Lignanos/farmacología , Masculino , Ratones , Neuralgia/complicaciones , Neuralgia/tratamiento farmacológico , Nervio Ciático/metabolismo , Médula Espinal/metabolismo , terc-Butilhidroperóxido/farmacología
5.
Planta Med ; 81(12-13): 1141-5, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-26287693

RESUMEN

The marine sponge-derived fungus Auxarthron reticulatum produces the cannabinoid receptor antagonist amauromine (1). Recultivation of the fungus to obtain further amounts for more detailed pharmacological evaluation of 1 additionally yielded the novel triterpene glycoside auxarthonoside (2), bearing, in nature, a rather rare sugar moiety, i.e., N-acetyl-6-methoxy-glucosamine. Amauromine (1), which inhibited cannabinoid CB1 receptors (Ki 0.178 µM) also showed antagonistic activity at the cannabinoid-like orphan receptor GPR18 (IC50 3.74 µM). The diketopiperazine 1 may thus serve as a lead structure for the development of more potent and selective GPR18 antagonists, which are required to study the orphan receptor's potential as a new drug target. Despite the execution of many biological assays, to date, no bioactivity could be found for auxarthonoside (2).


Asunto(s)
Alcaloides/química , Ascomicetos/química , Antagonistas de Receptores de Cannabinoides/química , Indoles/química , Poríferos/microbiología , Receptores Acoplados a Proteínas G/antagonistas & inhibidores , Triterpenos/antagonistas & inhibidores , Alcaloides/antagonistas & inhibidores , Alcaloides/aislamiento & purificación , Animales , Antagonistas de Receptores de Cannabinoides/aislamiento & purificación , Glicósidos/antagonistas & inhibidores , Humanos , Indoles/antagonistas & inhibidores , Indoles/aislamiento & purificación , Estructura Molecular , Receptor Cannabinoide CB1/antagonistas & inhibidores , Receptor Cannabinoide CB2/antagonistas & inhibidores
6.
Org Biomol Chem ; 9(12): 4487-97, 2011 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-21528140

RESUMEN

Several α-configured C-sialosides were synthesised by cross metathesis and further synthetic derivatisation to obtain ligands for Trypanosoma cruzi trans-sialidase (TcTS), a key enzyme in Chagas disease. Affinities of these compounds to immobilised TcTS were measured by surface plasmon resonance (SPR). The K(D) values thus obtained are in the lower millimolar range and will be discussed. The results show the importance of addressing Tyr(119) and Trp(312) side chains of TcTS in target oriented ligand synthesis, since these amino acids constitute the acceptor binding region in the active site of TcTS. The best ligand showed a significant decrease of TcTS activity in a preliminary NMR based inhibition assay.


Asunto(s)
Enfermedad de Chagas/tratamiento farmacológico , Química Farmacéutica/métodos , Inhibidores Enzimáticos/farmacología , Enzimas Inmovilizadas/antagonistas & inhibidores , Glicoproteínas/antagonistas & inhibidores , Glicósidos/antagonistas & inhibidores , Neuraminidasa/antagonistas & inhibidores , Ácidos Siálicos/química , Trypanosoma cruzi/efectos de los fármacos , Sitios de Unión , Dominio Catalítico , Enfermedad de Chagas/parasitología , Inhibidores Enzimáticos/síntesis química , Enzimas Inmovilizadas/química , Enzimas Inmovilizadas/metabolismo , Glicoproteínas/química , Glicoproteínas/metabolismo , Glicósidos/química , Glicósidos/metabolismo , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Cinética , Ligandos , Modelos Moleculares , Neuraminidasa/química , Neuraminidasa/metabolismo , Resonancia Magnética Nuclear Biomolecular , Unión Proteica , Ácidos Siálicos/metabolismo , Resonancia por Plasmón de Superficie , Trypanosoma cruzi/enzimología
7.
Comp Biochem Physiol C Toxicol Pharmacol ; 143(4): 436-43, 2006 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-16759914

RESUMEN

The free living ciliate Tetrahymena thermophila was chosen as a cellular model in order to investigate the mode of action of the anti-inflammatory marine natural product Pseudopterosin A (PsA). In this paper we present evidence that PsA inhibits phagosome formation (KD=10.5 microM) and triggers a discrete intracellular calcium release (depletion) from a site in T. thermophila cells (KD=6.4 microM). Pre-treatment with the Gi/o protein inhibitor, pertussis toxin (PTX), inhibits PsA activity of both responses providing pharmacological evidence that the site of action for PsA is at a PTX sensitive G protein or a G protein coupled receptor (GPCR). Addition of extracellular calcium induced a concentration dependent increase in the incidence of phagosome formation (KD=30.3 microM) and was blocked by PsA pre-treatment. This particular effect of PsA on extracellular calcium was not blocked by PTX pre-treatment.


Asunto(s)
Calcio/metabolismo , Diterpenos/farmacología , Glicósidos/farmacología , Toxina del Pertussis/farmacología , Fagocitosis/efectos de los fármacos , Animales , Cloruro de Calcio/farmacología , Diterpenos/antagonistas & inhibidores , Estrenos/farmacología , Proteínas de Unión al GTP/fisiología , Glicósidos/antagonistas & inhibidores , Fagosomas/efectos de los fármacos , Fagosomas/fisiología , Pirrolidinonas/farmacología , Tetrahymena thermophila/efectos de los fármacos
8.
Plant Physiol ; 122(2): 463-70, 2000 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10677439

RESUMEN

To investigate the mechanism by which fusicoccin (FC) induces the activation of the plasma membrane (PM) H(+)-ATPase, we used phenylarsine oxide (PAO), a known inhibitor of protein tyrosine-phosphatases. PAO was supplied in vivo in the absence or presence of FC to radish (Raphanus sativus L.) seedlings and cultured Arabidopsis cells prior to PM extraction. Treatment with PAO alone caused a slight decrease of PM H(+)-ATPase activity and, in radish, a decrease of PM-associated 14-3-3 proteins. When supplied prior to FC, PAO drastically inhibited FC-induced activation of PM H(+)-ATPase, FC binding to the PM, and the FC-induced increase of the amount of 14-3-3 associated with the PM. On the contrary, PAO was completely ineffective on all of the above-mentioned parameters when supplied after FC. The H(+)-ATPase isolated from PAO-treated Arabidopsis cells maintained the ability to respond to FC if supplied with exogenous, nonphosphorylated 14-3-3 proteins. Altogether, these results are consistent with a model in which the dephosphorylated state of tyrosine residues of a protein(s), such as 14-3-3 protein, is required to permit FC-induced association between the 14-3-3 protein and the PM H(+)-ATPase.


Asunto(s)
Arsenicales/farmacología , Glicósidos/antagonistas & inhibidores , ATPasas de Translocación de Protón/metabolismo , Brassica/enzimología , Membrana Celular/enzimología , Activación Enzimática , Glicósidos/farmacología
9.
Cancer Lett ; 55(3): 209-20, 1990 Dec 17.
Artículo en Inglés | MEDLINE | ID: mdl-2257539

RESUMEN

Solamargine [(22R,25R)-spiro-5-en-3 beta-yl-alpha-L-rhamnopyranosyl- (1----2glu)-O-alpha-L-rhamnopyranozyl (1----4glu)-beta-D-glucopyranoze], a glycoside of solasodine preferentially inhibits the uptake of tritiated thymidine by cancer cells. In contrast, solamargine at equivalent concentration, and the mono- and diglycosides of solasodine have a limited effect on the uptake of tritiated thymidine for other cell types, including unstimulated lymphocytes and lymphocytes stimulated with Con A. In contrast the solasodine glycosides do not inhibit the uptake of tritiated thymidine by lymphocytes stimulated with PHA or PWM. The inhibition of tritiated thymidine uptake by solamargine and the mono- and di-glycosides of solasodine are dependent upon their cellular uptake by endogenous endocytic lectins (EELs). The mode of action of the solasodine glycosides, in particular solamargine, appears to be the induction of cell lysis, as determined by morphological examination.


Asunto(s)
Glicósidos/farmacología , Alcaloides Solanáceos/farmacología , Secuencia de Carbohidratos , Carbohidratos/farmacología , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Femenino , Fibroblastos/citología , Fibroblastos/metabolismo , Glicósidos/antagonistas & inhibidores , Glicósidos/farmacocinética , Células HeLa/citología , Células HeLa/metabolismo , Humanos , Lectinas/metabolismo , Dosificación Letal Mediana , Activación de Linfocitos/efectos de los fármacos , Linfocitos/efectos de los fármacos , Datos de Secuencia Molecular , Neoplasias Ováricas/metabolismo , Neoplasias Ováricas/patología , Alcaloides Solanáceos/antagonistas & inhibidores , Alcaloides Solanáceos/farmacocinética , Timidina/farmacocinética , Tritio , Células Tumorales Cultivadas
10.
Res Commun Chem Pathol Pharmacol ; 53(1): 79-91, 1986 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-3749609

RESUMEN

Stevioside inhibits the action of atractyloside on energy metabolism in the isolated perfused rat liver. The effects of atractyloside on glycolysis, glycogenolysis, gluconeogenesis and oxygen uptake are decreased by stevioside. The concentration for half-maximal action is 0.5 mM. The site of the action is located on the outside of the cell. Possibly, stevioside affects the transport of atractyloside across the cell membrane.


Asunto(s)
Atractilósido/antagonistas & inhibidores , Diterpenos de Tipo Kaurano , Diterpenos , Glucósidos/farmacología , Glicósidos/antagonistas & inhibidores , Glicósidos/farmacología , Hígado/efectos de los fármacos , Terpenos/farmacología , Animales , Atractilósido/metabolismo , Atractilósido/toxicidad , Transporte Biológico Activo/efectos de los fármacos , Metabolismo Energético/efectos de los fármacos , Glucosa/metabolismo , Técnicas In Vitro , Hígado/metabolismo , Masculino , Consumo de Oxígeno/efectos de los fármacos , Perfusión , Ratas , Ratas Endogámicas , Edulcorantes/farmacología
15.
Proc Natl Acad Sci U S A ; 70(4): 1174-8, 1973 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-4197928

RESUMEN

The antiviral activity of the synthetic nucleoside, Virazole (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), against measles virus in Vero cell cultures was substantially reversed by xanthosine, guanosine, and to a slightly lesser extent by inosine. Virazole 5'-phosphate was subsequently found to be a potent competitive inhibitor of inosine 5'-phosphate dehydrogenase (IMP:NAD(+) oxidoreductase, EC 1.2.1.14) isolated from Escherichia coli (K(m) = 1.8 x 10(-5) M) with a K(i) of 2.7 x 10(-7) M. Guanosine 5'-phosphate (GMP) was a competitive inhibitor of this enzyme with a K(i) of 7.7 x 10(-5) M. Virazole 5'-phosphate was similarly active against IMP dehydrogenase isolated from Ehrlich ascites tumor cells, with a K(i) of 2.5 x 10(-7) M. The K(m) for this enzyme was 1.8 x 10(-5) M, and the K(i) for GMP was 2.2 x 10(-4) M. These results suggest that the antiviral activity of Virazole might be due to the inhibition of GMP biosynthesis in the infected cell at the step involving the conversion of IMP to xanthosine 5'-phosphate. This inhibition would consequently result in inhibition of the synthesis of vital viral nucleic acid.


Asunto(s)
Antivirales/farmacología , Glicósidos/farmacología , Virus del Sarampión/efectos de los fármacos , Fosfatasa Alcalina/metabolismo , Amidas/antagonistas & inhibidores , Amidas/metabolismo , Amidas/farmacología , Animales , Antivirales/antagonistas & inhibidores , Antivirales/metabolismo , Carcinoma de Ehrlich/enzimología , Línea Celular , Escherichia coli/enzimología , Glicósidos/antagonistas & inhibidores , Glicósidos/metabolismo , Guanosina/farmacología , Haplorrinos , Inosina/farmacología , Cetona Oxidorreductasas/antagonistas & inhibidores , Riñón , Hígado/metabolismo , Ratones , Nucleósidos/farmacología , Nucleótidos/farmacología , Ribonucleósidos/farmacología , Triazoles/antagonistas & inhibidores , Triazoles/metabolismo , Triazoles/farmacología , Tritio
20.
J Bacteriol ; 103(2): 422-5, 1970 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-4988242

RESUMEN

The arabinogalactan isolated from the cell wall of Mycobacterium bovis reacts to form a precipitate with concanavalin A, the phytohemagglutinin of the jack bean. A typical precipitin-like curve is obtained when increasing amounts of the polysaccharide are added to a constant quantity of concanavalin A. Inhibitor studies suggest that concanavalin A reacts with the arabinogalactan by interacting with the C-2, C-3, and C-5 hydroxyl groups of the alpha-d-arabinofuranosyl residues situated at the chain ends of this polysaccharide.


Asunto(s)
Pared Celular/análisis , Lectinas , Mycobacterium bovis/análisis , Polisacáridos/aislamiento & purificación , Precipitación Química , Glicósidos/antagonistas & inhibidores , Inmunodifusión
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