RESUMEN
We prepared network polysaccharide nanoscopic hydrogels by crosslinking water-soluble chitosan (WSCS) with a carboxylate-terminated maltooligosaccharide crosslinker via condensation. In this study, the enzymatic elongation of amylose chains on chitosan-based network polysaccharides by glucan phosphorylase (GP) catalysis was performed to obtain assembly materials. Maltoheptaose (Glc7) primers for GP-catalyzed enzymatic polymerization were first introduced into WSCS by reductive amination. Crosslinking of the product with the above-mentioned crosslinker by condensation was then performed to produce Glc7-modified network polysaccharides. The GP-catalyzed enzymatic polymerization of the α-d-glucose 1-phosphate monomer from the Glc7 primers on the network polysaccharides was conducted, where the elongated amylose chains formed double helices. Enzymatic disintegration of the resulting network polysaccharide assembly successfully occurred by α-amylase-catalyzed hydrolysis of the double helical amyloses. The encapsulation and release of a fluorescent dye, Rhodamine B, using the CS-based network polysaccharides were also achieved by means of the above two enzymatic approaches.
Asunto(s)
Quitosano , Colorantes Fluorescentes , Glucanos , Polisacáridos , Quitosano/química , Colorantes Fluorescentes/química , Polisacáridos/química , Rodaminas/química , Hidrogeles/química , alfa-Amilasas/química , alfa-Amilasas/metabolismo , Hidrólisis , Amilosa/química , Polimerizacion , Oligosacáridos/química , Glucofosfatos/química , Glucofosfatos/metabolismoRESUMEN
Glycogen phosphorylase (GP) is a key enzyme in the glycogenolysis pathway and a potential therapeutic target in the management of type 2 diabetes. It catalyzes a reversible reaction: the release of the terminal glucosyl residue from glycogen as glucose 1-phosphate; or the transfer of glucose from glucose 1-phosphate to glycogen. A colorimetric method to follow in vitro the activity of GP with usefulness in structure-activity relationship studies and high-throughput screening capability is herein described. The obtained results allowed the choice of the optimal concentration of enzyme of 0.38 U/mL, 0.25 mM glucose 1-phosphate, 0.25 mg/mL glycogen, and temperature of 37 °C. Three known GP inhibitors, CP-91149, a synthetic inhibitor, caffeine, an alkaloid, and ellagic acid, a polyphenol, were used to validate the method, CP-91149 being the most active inhibitor. The effect of glucose on the IC50 value of CP-91149 was also investigated, which decreased when the concentration of glucose increased. The assay parameters for a high-throughput screening method for discovery of new potential GP inhibitors were optimized and standardized, which is desirable for the reproducibility and comparison of results in the literature. The optimized method can be applied to the study of a panel of synthetic and/or natural compounds, such as polyphenols.
Asunto(s)
Glucosa/química , Glucofosfatos/química , Glucógeno Fosforilasa/química , Glucógeno/química , Amidas/farmacología , Animales , Cafeína/farmacología , Ácido Elágico/farmacología , Pruebas de Enzimas , Glucógeno Fosforilasa/antagonistas & inhibidores , Glucógeno Fosforilasa/aislamiento & purificación , Ensayos Analíticos de Alto Rendimiento , Indoles/farmacología , Cinética , Conejos , Soluciones , Relación Estructura-ActividadRESUMEN
Here, we report a biochemical characterization of recombinant maize indole-3-acetyl-ß-d-glucose (IAGlc) synthase which glucosylates indole-3-acetic acid (IAA) and thus abolishes its auxinic activity affecting plant hormonal homeostasis. Substrate specificity analysis revealed that IAA is a preferred substrate of IAGlc synthase; however, the enzyme can also glucosylate indole-3-butyric acid and indole-3-propionic acid with the relative activity of 66% and 49.7%, respectively. KM values determined for IAA and UDP glucose are 0.8 and 0.7 mM, respectively. 2,4-Dichlorophenoxyacetic acid is a competitive inhibitor of the synthase and causes a 1.5-fold decrease in the enzyme affinity towards IAA, with the Ki value determined as 117 µM, while IAA-Asp acts as an activator of the synthase. Two sugar-phosphate compounds, ATP and glucose-1-phosphate, have a unique effect on the enzyme by acting as activators at low concentrations and showing inhibitory effect at higher concentrations (above 0.6 and 4 mM for ATP and glucose-1-phosphate, respectively). Results of molecular docking revealed that both compounds can bind to the PSPG (plant secondary product glycosyltransferase) motif of IAGlc synthase; however, there are also different potential binding sites present in the enzyme. We postulate that IAGlc synthase may contain more than one binding site for ATP and glucose-1-phosphate as reflected in its activity modulation.
Asunto(s)
Glucosiltransferasas/química , Uridina Difosfato Glucosa/química , Zea mays/enzimología , Ácido 2,4-Diclorofenoxiacético/química , Adenosina Trifosfato/química , Secuencias de Aminoácidos , Sitios de Unión , Cationes , Inhibidores Enzimáticos/farmacología , Escherichia coli/metabolismo , Glucosa/química , Glucofosfatos/química , Glucosiltransferasas/antagonistas & inhibidores , Homeostasis , Concentración de Iones de Hidrógeno , Cinética , Simulación del Acoplamiento Molecular , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas Recombinantes/química , Especificidad por Sustrato , Zea mays/efectos de los fármacosRESUMEN
Phosphodiesters of glucose-2-phosphate (G2P) are found only in few natural compounds such as agrocinopine D and agrocin 84. Agrocinopine D is a G2P phosphodiester produced by plants infected by Agrobacterium fabrum C58 and recognized by the bacterial periplasmic binding protein AccA for being transported into the bacteria before cleavage by the phosphodiesterase AccF, releasing G2P, which promotes virulence by binding the repressor protein AccR. The G2P amide agrocin 84 is a natural antibiotic produced by the non-pathogenic Agrobacterium radiobacter K84 strain used as a biocontrol agent by competing with Agrobacterium fabrum C58. G2P esters are also found in irregular glycogen structures. The rare glucopyranosyl-2-phophoryl moiety found in agrocin 84 is the key structural signature enabling its action as a natural antibiotic. Likewise, G2P and G2P esters can also dupe the Agrobacterium agrocinopine catabolism cascade. Such observations illustrate the importance of G2P esters on which we have recently focused our interest. After a brief review of the reported phosphorylation coupling methods and the choice of carbohydrate building blocks used in G2P chemistry, a flexible access to glucose-2-phosphate esters using the phosphoramidite route is proposed.
Asunto(s)
Nucleótidos de Adenina , Agrobacterium , Glucofosfatos , Glucógeno , Nucleótidos de Adenina/química , Nucleótidos de Adenina/metabolismo , Agrobacterium/química , Agrobacterium/metabolismo , Ésteres/química , Ésteres/metabolismo , Glucofosfatos/química , Glucofosfatos/metabolismo , Glucógeno/química , Glucógeno/metabolismo , Proteínas de Unión Periplasmáticas/metabolismoRESUMEN
Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1â6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae). The structures of these polymers were described for the first time in prokaryotes. Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and ß-D-glucopyranose residues in the main chain, â6)-ß-D-Glcp-(1â8)-α-Kdn-(2â, has been previously detected in a number of actinobacteria. The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.
Asunto(s)
Pared Celular/metabolismo , Glucofosfatos/metabolismo , Espectroscopía de Resonancia Magnética/métodos , Micrococcaceae/metabolismo , Polímeros/química , Polisacáridos Bacterianos/metabolismo , Ácidos Teicoicos/metabolismo , Pared Celular/química , Glucofosfatos/química , Polisacáridos Bacterianos/química , Ácidos Teicoicos/químicaRESUMEN
The first non-natural derivative of the rare d-glucose-2-phosphate (G2P), namely glucose-2-(O-lactic acid phosphate) (G2LP), has been synthesized. When used as sole carbon source, G2LP enables bacterial growth of the plant pathogenic strain Agrobacterium fabrum C58 (formerly referred to as Agrobacterium tumefaciens). X-ray crystallography and affinity measurements investigations reveal that G2LP binds the periplasmic binding protein (PBP) AccA similarly to the natural compounds and with the same affinity. Moreover, enzymatic assays show that it is able to serve as substrate of the phosphodiesterase AccF. The properties found for G2LP demonstrate that the very unusual glucose-2-phosphoryl residue, present in G2LP, can be used as structural feature for designing non-natural systems fully compatible with the Acc cascade of A. fabrum.
Asunto(s)
Agrobacterium/química , Proteínas Bacterianas/metabolismo , Ésteres/síntesis química , Glucofosfatos/síntesis química , Proteínas de Unión Periplasmáticas/metabolismo , Agrobacterium/crecimiento & desarrollo , Cristalografía por Rayos X , Ésteres/química , Ésteres/metabolismo , Glucofosfatos/química , Glucofosfatos/metabolismo , Hidrolasas Diéster Fosfóricas/metabolismo , Especificidad por SustratoRESUMEN
Phosphorylated carbohydrates are central metabolites involved in key plant metabolic pathways, such as glycolysis and central carbon metabolism. Such pathways influence plant growth, development, and stress responses to environmental changes, and ultimately, reflect the plant's energy status. The high polarity of these metabolites, the variety of isomeric structures (e.g., glucose-1-phosphate (G1P)/fructose-6-phosphate (F6P)/mannose-6-phosphate (M6P)/G6P, sucrose-6-phosphate (S6P)/T6P), and rapid metabolic turnover makes their analysis particularly challenging. In this chapter, we describe the use of a set of known phosphorylated carbohydrates to develop and validate a hydrophilic interaction chromatography (HILIC) triple quadrupole (QqQ) tandem mass spectrometry (MS/MS) method in the highly sensitive and selective multiple reaction monitoring (MRM) mode for the target analysis of G1P, F6P, M6P, G6P, S6P, T6P, and the sugar nucleotide uridine 5-diphospho-glucose (UDPG). We present detailed information regarding HILIC column chemistry and practical considerations when coupling it with a QqQ-MS system.
Asunto(s)
Sacarosa/análogos & derivados , Fosfatos de Azúcar/análisis , Espectrometría de Masas en Tándem/métodos , Trehalosa/análogos & derivados , Metabolismo de los Hidratos de Carbono , Glucofosfatos/análisis , Glucofosfatos/química , Interacciones Hidrofóbicas e Hidrofílicas , Sacarosa/análisis , Sacarosa/química , Fosfatos de Azúcar/química , Trehalosa/análisis , Trehalosa/químicaRESUMEN
Novel teichulosonic acid with the repeating unit â6)-ß-D-GlcpNAc-(1â8)-α-Kdn-(2â has been found in the cell walls of two Arthrobacter strains, VKM Ac-2549 and VKM Ac-2550. The teichulosonic acid was revealed in representatives of the genus Arthrobacter for the first time. Two other polymers identified in the above strains were poly(monoglycosyl 1-phosphate) and poly(diglycosyl 1-phosphate) of hitherto unknown structures, i.e., -6)-α-D-GalpNAc-(1-P-, and -6)-ß-D-GlcpNAc-(1â3)-α-D-Galp-(1-P-. The structures of all three polymers were established by using chemical, NMR spectroscopic and ESI-MS methods. The strains studied in this work differ in the cell wall composition from the type strain of phylogenetically closely related species A. crystallopoietes which was reported to contain a teichoic acid and supposedly had a glycosyl 1-phosphate polymer.
Asunto(s)
Arthrobacter/química , Pared Celular/química , Glucofosfatos/química , Polímeros/química , Ácidos Teicoicos/química , FilogeniaRESUMEN
Erwinia amylovora, a Gram-negative plant pathogen, is the causal agent of Fire Blight, a contagious necrotic disease affecting plants belonging to the Rosaceae family, including apple and pear. E. amylovora is highly virulent and capable of rapid dissemination in orchards; effective control methods are still lacking. One of its most important pathogenicity factors is the exopolysaccharide amylovoran. Amylovoran is a branched polymer made by the repetition of units mainly composed of galactose, with some residues of glucose, glucuronic acid and pyruvate. E. amylovora glucose-1-phosphate uridylyltransferase (UDP-glucose pyrophosphorylase, EC 2.7.7.9) has a key role in amylovoran biosynthesis. This enzyme catalyses the production of UDP-glucose from glucose-1-phosphate and UTP, which the epimerase GalE converts into UDP-galactose, the main building block of amylovoran. We determined EaGalU kinetic parameters and substrate specificity with a range of sugar 1-phosphates. At time point 120min the enzyme catalysed conversion of the sugar 1-phosphate into the corresponding UDP-sugar reached 74% for N-acetyl-α-d-glucosamine 1-phosphate, 28% for α-d-galactose 1-phosphate, 0% for α-d-galactosamine 1-phosphate, 100% for α-d-xylose 1-phosphate, 100% for α-d-glucosamine 1-phosphate, 70% for α-d-mannose 1-phosphate, and 0% for α-d-galacturonic acid 1-phosphate. To explain our results we obtained the crystal structure of EaGalU and augmented our study by docking the different sugar 1-phosphates into EaGalU active site, providing both reliable models for substrate binding and enzyme specificity, and a rationale that explains the different activity of EaGalU on the sugar 1-phosphates used. These data demonstrate EaGalU potential as a biocatalyst for biotechnological purposes, as an alternative to the enzyme from Escherichia coli, besides playing an important role in E. amylovora pathogenicity.
Asunto(s)
Proteínas Bacterianas/química , Erwinia amylovora/enzimología , Glucofosfatos/química , UTP-Glucosa-1-Fosfato Uridililtransferasa/química , Uridina Difosfato Glucosa/química , Uridina Trifosfato/química , Acetilglucosamina/análogos & derivados , Acetilglucosamina/química , Acetilglucosamina/metabolismo , Secuencia de Aminoácidos , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Cristalografía por Rayos X , Erwinia amylovora/química , Escherichia coli/genética , Escherichia coli/metabolismo , Galactosamina/análogos & derivados , Galactosamina/química , Galactosamina/metabolismo , Galactosafosfatos/química , Galactosafosfatos/metabolismo , Expresión Génica , Glucosamina/análogos & derivados , Glucosamina/química , Glucosamina/metabolismo , Glucofosfatos/metabolismo , Cinética , Manosafosfatos/química , Manosafosfatos/metabolismo , Modelos Moleculares , Simulación del Acoplamiento Molecular , Pentosafosfatos/química , Pentosafosfatos/metabolismo , Polisacáridos Bacterianos/biosíntesis , Polisacáridos Bacterianos/química , Dominios y Motivos de Interacción de Proteínas , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad por Sustrato , UTP-Glucosa-1-Fosfato Uridililtransferasa/genética , UTP-Glucosa-1-Fosfato Uridililtransferasa/metabolismo , Uridina Difosfato Glucosa/metabolismo , Uridina Trifosfato/metabolismoRESUMEN
BACKGROUND: The biosynthesis of NDP-glucoses is based on the nucleotide transfer from NTP donor substrates to glucose-1-phosphates catalyzed by glucose-1-phosphate nucleotidyltransferases. OBJECTIVES: The cloning and biochemical characterization of a glucose-1-phosphate nucleotidyltransferase (TiGPNT) from the deep sea bacterium Thermodesulfatator indicus. METHODS: The biochemical parameters of recombinant TiGPNT were determined using a plate reader-based coupled enzymatic assay, in which the reaction product UDP-glucose is oxidized in the presence of NAD+ forming UDP-Glucuronic acid and NADH. The substrate promiscuity of the enzyme was determined using thin-layer chromatography and MALDI-ToF mass spectrometry. RESULTS: TiGPNT was recombinantly expressed under the control of the T7 promoter in Escherichia coli and could be successfully enriched by heat treatment at 80°C for 30 min. The obtained enzyme worked best at pH 7.5 and the optimum reaction temperature was determined to be 50°C. Interestingly, TiGPNT could fully retain its activity even after extended incubation periods at temperatures of up to 80°C. The enzyme was strongly inhibited in the presence of Cu2+ and Fe2+ ions and EDTA. Among the tested glycosyl donor substrates, TiGPNT showed strict specificity towards glucose-1-phosphate. At the same time, TiGPNT was highly promiscuous towards all tested nucleotide donor substrates. CONCLUSION: TiGPNT shows comparable biochemical features in regards to pH optima, temperature optima and the substrate specificity to characterized glucose-1-phosphate nucleotidyltransferase from other species. The enzyme was capable of utilizing glucose-1-phosphate and all tested nucleoside triphosphate donors as substrates. The high activity of the enzyme and the simple purification protocol make TiGPNT an interesting new biocatalyst for the synthesis of glucose-diphospho nucleosides.
Asunto(s)
Bacterias/química , Proteínas Bacterianas/metabolismo , Glucofosfatos/química , NAD/química , Uridina Difosfato Glucosa/química , Organismos Acuáticos , Bacterias/enzimología , Proteínas Bacterianas/genética , Clonación Molecular , Estabilidad de Enzimas , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Glucofosfatos/metabolismo , Calor , Concentración de Iones de Hidrógeno , Cinética , NAD/metabolismo , Nucleotidiltransferasas/genética , Nucleotidiltransferasas/metabolismo , Plásmidos/química , Plásmidos/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Especificidad por Sustrato , Uridina Difosfato Glucosa/metabolismoRESUMEN
Enzymes in the α-d-phosphohexomutases superfamily catalyze the reversible conversion of phosphosugars, such as glucose 1-phosphate and glucose 6-phosphate. These reactions are fundamental to primary metabolism across the kingdoms of life and are required for a myriad of cellular processes, ranging from exopolysaccharide production to protein glycosylation. The subject of extensive mechanistic characterization during the latter half of the 20th century, these enzymes have recently benefitted from biophysical characterization, including X-ray crystallography, NMR, and hydrogen-deuterium exchange studies. This work has provided new insights into the unique catalytic mechanism of the superfamily, shed light on the molecular determinants of ligand recognition, and revealed the evolutionary conservation of conformational flexibility. Novel associations with inherited metabolic disease and the pathogenesis of bacterial infections have emerged, spurring renewed interest in the long-appreciated functional roles of these enzymes.
Asunto(s)
Glucofosfatos/metabolismo , Fosfoglucomutasa/química , Fosfoglucomutasa/metabolismo , Secuencia de Aminoácidos , Animales , Bacterias/química , Bacterias/enzimología , Bacterias/genética , Bacterias/metabolismo , Infecciones Bacterianas/enzimología , Infecciones Bacterianas/genética , Infecciones Bacterianas/metabolismo , Dominio Catalítico , Cristalografía por Rayos X , Glucofosfatos/química , Glucofosfatos/genética , Humanos , Enfermedades Metabólicas/enzimología , Enfermedades Metabólicas/genética , Enfermedades Metabólicas/metabolismo , Mutación , Resonancia Magnética Nuclear Biomolecular , Fosfoglucomutasa/genética , Conformación Proteica , Alineación de SecuenciaRESUMEN
The idea that hydrogen bond cooperativity is responsible for the structure and reactivity of carbohydrates is examined. Density functional theory and gauge-including atomic orbital calculations on the known conformers of the α and ß anomers of d-glucopyranose in the gas phase are used to compute proton NMR chemical shifts and interatomic distances, which are taken as criteria for probing intramolecular interactions. Atom-atom interaction energies are calculated by the interacting quantum atoms approach in the framework of the quantum theory of atoms in molecules. Association of OH1 in the counterclockwise conformers with a strong acceptor, pyridine, is accompanied by cooperative participation from OH2, but there is no significant change in the bonding of the two following 1,2-diol motifs. The OH6... O5 (G-g+/cc/t and G+g-/cc/t conformers) or OH6... O4 (Tg+/cc/t conformer) distance is reduced, and the OH6 proton is slightly deshielded. In the latter case, this shortening and the associated increase in the OH6-O4 interaction energy may be interpreted as a small cooperative effect, but intermolecular interaction energies are practically the same for all three conformers. In most of the pyridine complexes, one ortho proton interacts with the endocyclic oxygen O5. Analogous results are obtained when the clockwise conformer, G-g+/cl/g-, detected for the α anomer, and a hypothetical conformer, Tt/cl/g-, are complexed with pyridine through OH6. Generally, the cooperative effect does not go beyond the first two OH groups of a chain. Copyright © 2017 John Wiley & Sons, Ltd.
Asunto(s)
Glucosa/química , Espectroscopía de Resonancia Magnética , Teoría Cuántica , Glucofosfatos/química , Enlace de Hidrógeno , Conformación Molecular , Estructura MolecularRESUMEN
Non-covalent complexes (NCC) between hexose monophosphates (HexP) and arginine (R) were analyzed using ESI MS and MS/MS in negative mode under different (hard, HC and soft, SC) desolvation conditions. High resolution mass spectrometry (HRMS) revealed the presence of different ionic species, namely, homo- and heteromultimers of R and HexP. Deprotonated heterodimers and corresponding sodiated species were enhanced under HC likely due to a decrease in available charge number associated with the reduction of H+/Na+ exchange. The quantum calculations showed that the formation of covalent systems is very little exothermic, therefore, such systems are disfavored. Desolvation dependent CID spectra of deprotonated [(HexP+R)âH]- complexes demonstrated that they can exist within the hydrogen bond (HB) and salt bridge (SB) forms, yielding either NCC separation or covalent bond cleavages, respectively. Although HB forms are the main species, they cannot survive under HC; therefore, the minor SB forms became detectable. Energy-resolved mass spectrometry (ERMS) experiments revealed diagnostic fragment ions from both SB and HB forms, providing evidence that these isomeric forms are inconvertible. SB formation should result from the ionic interactions of highly acidic group of HexP with strongly basic guanidine group of arginine and thus requires an arginine zwitterion (ZW) form. This was confirmed by quantum calculations. Ion-ion interactions are significantly affected by the presence of sodium cation as demonstrated by the fragmentation patterns of sodiated complex species. Regarding CID data, only SB between protonated amino group of R and deprotonated phosphate group of HexP could be suggested, but the primary amine is not enough basic then, the SB must be fleeting. Nevertheless, the observation of the covalent bond cleavages suggests the presence of structures with a free negative charge able to induce fragmentations. Indeed, according to quantum calculations, solvated salt (SS) systems involving Na+/COO- salt solvated by neutral phosphate and negative charge on sugar ring are preferentially formed.
Asunto(s)
Arginina/química , Fructosafosfatos/química , Glucosa-6-Fosfato/química , Glucofosfatos/química , Enlace de Hidrógeno , Isomerismo , Modelos Moleculares , Espectrometría de Masa por Ionización de Electrospray , Espectrometría de Masas en Tándem , TermodinámicaRESUMEN
α-Glucose 1-phosphate (G1P) is synthesized from 5% (w/v) corn starch and 1 M phosphate mediated by α-glucan phosphorylase (αGP) from the thermophilic bacterium Thermotoga maritima at pH 7.2 and 70 °C. To increase G1P yield from corn starch containing branched amylopectin, a hyper-thermostable isoamylase from Sulfolobus tokodaii was added for simultaneous starch gelatinization and starch-debranching hydrolysis at 85 °C and pH 5.5 before αGP use. The pretreatment of isoamylase increased G1P titer from 120 mM to 170 mM. To increase maltose and maltotriose utilization, the third thermostable enzyme, 4-glucanotransferase (4GT) from Thermococcus litoralis, was added during the late stage of G1P biotransformation, further increasing G1P titer to 200 mM. This titer is the highest G1P level obtained on starch or its derived products (maltodextrin and soluble starch). This study suggests that in vitro multienzyme biotransformation has an advantage of great engineering flexibility in terms of space and time compared with microbial fermentation.
Asunto(s)
Proteínas Arqueales/química , Proteínas Bacterianas/química , Glucofosfatos/química , Glucosiltransferasas/química , Isoamilasa/química , Fosforilasas/química , Almidón/química , Biocatálisis , Calor , Concentración de Iones de Hidrógeno , Sulfolobus/química , Sulfolobus/enzimología , Thermococcus/química , Thermococcus/enzimología , Thermotoga maritima/química , Thermotoga maritima/enzimologíaRESUMEN
Thermostable α-glucan phosphorylase-catalyzed enzymatic copolymerization of α-d-glucose 1-phosphate (Glc-1-P) with its analogue monomer, α-d-glucosamine 1-phosphate (GlcN-1-P), from a maltotriose primer was performed to produce non-natural heteroaminopolysaccharides composed of Glc/GlcN units, that is, α(1â4)-linked glucosaminoglucans. The GlcN units in the products were further converted to N-acetyl-d-glucosamine (GlcNAc) units by N-acetylation. The structures of the products were evaluated by the MALDI-TOF MS, (1)H NMR, and (1)H-(1)H COSY NMR measurements, which were completely different from those of the natural glycosaminoglycans. The degrees of polymerization and Glc/GlcN compositional ratios of the products were relatively dependent on the Glc-1-P/Glc-1-P/Glc3 feed ratios. The noncrystalline natures of the present materials were supported by the X-ray diffraction measurement.
Asunto(s)
Proteínas Bacterianas/química , Glicosaminoglicanos/química , Fosforilasas/química , Polisacáridos/síntesis química , Acetilación , Acetilglucosamina/química , Proteínas Bacterianas/aislamiento & purificación , Biocatálisis , Secuencia de Carbohidratos , Estabilidad de Enzimas , Glucosamina/análogos & derivados , Glucosamina/química , Glucofosfatos/química , Calor , Datos de Secuencia Molecular , Fosforilasas/aislamiento & purificación , Polimerizacion , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
Substrate binding properties of the large (LS) and small (SS) subunits of potato tuber ADP-glucose pyrophosphorylase were investigated by using isothermal titration calorimetry. Our results clearly show that the wild type heterotetramer (S(WT)L(WT)) possesses two distinct types of ATP binding sites, whereas the homotetrameric LS and SS variant forms only exhibited properties of one of the two binding sites. The wild type enzyme also exhibited significantly increased affinity to this substrate compared to the homotetrameric enzyme forms. No stable binding was evident for the second substrate, glucose-1-phosphate, in the presence or absence of ATPγS suggesting that interaction of glucose-1-phosphate is dependent on hydrolysis of ATP and supports the Theorell-Chance bi bi reaction mechanism.
Asunto(s)
Calorimetría/métodos , Glucosa-1-Fosfato Adenililtransferasa/metabolismo , Proteínas de Plantas/metabolismo , Tubérculos de la Planta/enzimología , Solanum tuberosum/enzimología , Adenosina Trifosfato/análogos & derivados , Adenosina Trifosfato/química , Adenosina Trifosfato/metabolismo , Sitios de Unión , Unión Competitiva , Western Blotting , Glucosa-1-Fosfato Adenililtransferasa/química , Glucofosfatos/química , Glucofosfatos/metabolismo , Cinética , Modelos Moleculares , Estructura Molecular , Proteínas de Plantas/química , Unión Proteica , Multimerización de Proteína , Estructura Terciaria de Proteína , Subunidades de Proteína/química , Subunidades de Proteína/metabolismo , Especificidad por Sustrato , TermodinámicaRESUMEN
The relationship between two aminopolysaccharide stereoisomers, namely α-(1â4)- and ß-(1â4)-linked (N-acetyl)-D-glucosamine polymers, is of significant interest within the field of polysaccharide science, as they correspond to amino analogs of the representative abundant natural polysaccharides, viz. amylose and cellulose. While the latter glucosamine polymer is the basis of well-known natural polysaccharides, chitin and chitosan (linear polysaccharides composed of ß-(1â4)-linked N-acetyl-D-glucosamine and D-glucosamine), to the best of our knowledge, the former (α-(1â4)-linked) has not been observed in nature. For the purpose of these studies, the synthesis of such non-natural aminopolysaccharides was performed by the thermostable α-glucan phosphorylase (from Aquifex aeolicus VF5)-catalyzed enzymatic polymerization of α-D-glucosamine 1-phosphate (GlcN-1-P), via successive α-glucosaminylations, in ammonia buffer containing Mg(2+) ions, resulting in the production of the α-(1â4)-linked D-glucosamine polymers, corresponding to the structure of the chitosan stereoisomer. Subsequent N-acetylation of the products gave the aminopolysaccharides, corresponding to the chitin stereoisomer.
Asunto(s)
Quitina/química , Quitina/síntesis química , Quitosano/química , Quitosano/síntesis química , Glucosamina/análogos & derivados , Glucofosfatos/química , Fosforilasas/metabolismo , Polimerizacion , Aquifoliaceae/enzimología , Biocatálisis , Técnicas de Química Sintética , Estabilidad de Enzimas , Glucosamina/química , Fosforilasas/química , Estereoisomerismo , TemperaturaRESUMEN
Cps2L, a thymidylytransferase, is the first enzyme in Streptococcus pneumoniae L-rhamnose biosynthesis and an antibacterial target. We herein report the evaluation of six sugar phosphate analogues selected to further probe Cps2L substrate tolerance. A modified continuous spectrophotometric assay was employed for facile detection of pyrophosphate (PPi) released from nucleotidylyltransfase-catalysed condensation of sugar 1-phosphates and nucleoside triphosphates to produce sugar nucleotides. Additionally, experiments using waterLOGSY NMR spectroscopy were investigated as a complimentary method to evaluate binding affinity to Cps2L.
Asunto(s)
Antibacterianos/química , Proteínas Bacterianas/química , Inhibidores Enzimáticos/química , Glucofosfatos/química , Nucleotidiltransferasas/química , Antibacterianos/síntesis química , Proteínas Bacterianas/antagonistas & inhibidores , Difosfatos/análisis , Pruebas de Enzimas , Inhibidores Enzimáticos/síntesis química , Cinética , Nucleotidiltransferasas/antagonistas & inhibidores , Proteínas Recombinantes/química , Espectrofotometría , Streptococcus pneumoniae/química , Streptococcus pneumoniae/enzimologíaRESUMEN
ß-Phosphoglucomutase (ßPGM) catalyzes isomerization of ß-D-glucose 1-phosphate (ßG1P) into D-glucose 6-phosphate (G6P) via sequential phosphoryl transfer steps using a ß-D-glucose 1,6-bisphosphate (ßG16BP) intermediate. Synthetic fluoromethylenephosphonate and methylenephosphonate analogs of ßG1P deliver novel step 1 transition state analog (TSA) complexes for ßPGM, incorporating trifluoromagnesate and tetrafluoroaluminate surrogates of the phosphoryl group. Within an invariant protein conformation, the ß-D-glucopyranose ring in the ßG1P TSA complexes (step 1) is flipped over and shifted relative to the G6P TSA complexes (step 2). Its equatorial hydroxyl groups are hydrogen-bonded directly to the enzyme rather than indirectly via water molecules as in step 2. The (C)O-P bond orientation for binding the phosphate in the inert phosphate site differs by â¼ 30° between steps 1 and 2. By contrast, the orientations for the axial O-Mg-O alignment for the TSA of the phosphoryl group in the catalytic site differ by only â¼ 5°, and the atoms representing the five phosphorus-bonded oxygens in the two transition states (TSs) are virtually superimposable. The conformation of ßG16BP in step 1 does not fit into the same invariant active site for step 2 by simple positional interchange of the phosphates: the TS alignment is achieved by conformational change of the hexose rather than the protein.
Asunto(s)
Hexosas/química , Hexosas/metabolismo , Organofosfonatos/química , Organofosfonatos/metabolismo , Fosfoglucomutasa/química , Fosfoglucomutasa/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Catálisis , Cristalografía por Rayos X , Flúor/química , Glucosa-6-Fosfato/química , Glucosa-6-Fosfato/metabolismo , Glucofosfatos/química , Glucofosfatos/metabolismo , Isomerismo , Cinética , Lactococcus lactis/enzimología , Magnesio/química , Modelos Moleculares , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Conformación Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Electricidad Estática , TermodinámicaRESUMEN
This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 â 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively. The structure of the products is confirmed by the (1)H NMR analysis. The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement. These materials self-assemble in water at pH = pI to form large aggregates, but disassemble at pH shifted from pI.