Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Más filtros












Base de datos
Intervalo de año de publicación
1.
Phytochem Anal ; 30(2): 156-163, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30426588

RESUMEN

INTRODUCTION: In the present study, a green and efficient extraction method using deep eutectic solvents as extraction solvent was developed for extracting the four major active compounds narirutin, naringin, hesperidin and neohesperidin from Aurantii Fructus. METHODOLOGY: A series of tunable deep eutectic solvents were prepared and investigated by mixing choline chloride or betaine to different hydrogen-bond donors, and betaine/ethanediol was found to be the most suitable extraction solvent. To achieve the best extraction yield, the primary factors affecting the extraction efficiency, such as hydrogen-bond acceptor/hydrogen-bond donor ratio, water content in deep eutectic solvents, extraction temperature, solid/liquid ratio and extraction time, were investigated. RESULTS: The optimal extraction conditions were 40% of water in betaine/ethanediol (1:4) at 60°C for heated extraction of 30 min and solid/liquid ratio 1:100 g/mL. Under the optimum extraction condition, the extraction yields of narirutin, naringin, hesperidin, and neohesperidin were 8.39 ± 0.61, 83.98 ± 1.92, 3.03 ± 0.35 and 35.94 ± 0.63 mg/g, respectively, which were much higher than those of methanol as extraction solvent (5.5 ± 0.48, 64.23 ± 1.51, 2.16 ± 0.15 and 30.14 ± 0.62 mg/g). CONCLUSION: The present results showed that deep eutectic solvents could be promising green and efficient solvents for extraction of the bioactive ingredients from traditional Chinese medicine.


Asunto(s)
Disacáridos/aislamiento & purificación , Flavanonas/aislamiento & purificación , Tecnología Química Verde , Hesperidina/análogos & derivados , Hesperidina/aislamiento & purificación , Solventes/química , Cromatografía Líquida de Alta Presión/métodos , Disacáridos/normas , Flavanonas/normas , Hesperidina/normas , Enlace de Hidrógeno , Estándares de Referencia , Espectrofotometría Ultravioleta/métodos
2.
J Sci Food Agric ; 95(9): 1885-91, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25170574

RESUMEN

BACKGROUND: Neohesperidin is an important natural flavanone glycoside distributed in several citrus species. This compound is widely used as a raw material for food additives in the food industry. The request for certified reference materials (CRMs) in dietary supplements was stipulated by the National Administrative Committee for CRMs and was underpinned by the need to improve the accuracy and comparability of measurement data and to establish metrological traceability of analytical results. RESULTS: This paper reports the sample preparation methodology, homogeneity and stability studies, value assignment and uncertainty estimation of a new certified reference material of neohesperidin (GBW09522). Differential scanning calorimetry, coulometric titration and mass balance methods proved to be sufficiently reliable and accurate for certification purposes. The certified value of neohesperidin CRM is 994 g kg(-1) with an expanded uncertainty of 4 g kg(-1) (k = 2). The reference material described above was homogeneous and stable for 12 months at a storage temperature of 25 °C. CONCLUSION: The new CRM of neohesperidin can be used to validate analytical methods and improve the accuracy of measurement data as well as quality control of neohesperidin-related dietary supplements, foods, traditional herbs and pharmaceutical formulations.


Asunto(s)
Antioxidantes/análisis , Suplementos Dietéticos/análisis , Inspección de Alimentos , Hesperidina/análogos & derivados , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Antioxidantes/normas , Calibración , Rastreo Diferencial de Calorimetría , China , Cromatografía Líquida de Alta Presión , Hesperidina/análisis , Hesperidina/química , Hesperidina/aislamiento & purificación , Hesperidina/normas , Peso Molecular , Control de Calidad , Estándares de Referencia , Reproducibilidad de los Resultados , Espectrofotometría Ultravioleta , Factores de Tiempo , Volumetría , Incertidumbre
3.
J AOAC Int ; 91(3): 506-10, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-18567294

RESUMEN

Phenolic phytochemical are known to perform several functions ranging from phytoprotectants, to protecting lipids in food products, to antioxidant activity in animals and humans. The need for a common standard mixture containing multiple phenolic phytochemicals is critical for the development of a robust validation assay for accurately quantifying antioxidant activity in various matrixes. Different research groups have used A wide array of single purified reference phenolic compounds in this regard. A 5 compound mixture (caffeic acid, morin hydrate, hesperetin, catechin hydrate, and epigallocatechin gallate) containing phenolic compounds from 4 subgroups (phenolic acid, flavone, flavanone, and flavan-3-ol) was prepared. The mixture was assayed for stability evaluation by high-performance liquid chromatography (HPLC) using a diode array detection procedure for a 3 month time interval. HPLC analysis confirmed that there was no significant interaction between different components of the mixture. The among-sample relative standard deviation (RSD) of all 5 phenolic compounds, as well as the total HPLC area, was < 1%. The RSD due to instrument variation was < 2% and the total RSD among-days was < 5%. These results unambiguously suggest that the sample was stable for a 3 month time interval in an amber vial stored in a refrigerator below 5 degrees C. This mixture is currently being used for the single-laboratory validation study for the assay of total phenolic content by the Folin-Ciocalteu method and the antioxidant capacity by oxygen radical absorbing capacity procedure.


Asunto(s)
Antioxidantes/análisis , Antioxidantes/normas , Fenoles/análisis , Fenoles/normas , Ácidos Cafeicos/análisis , Ácidos Cafeicos/normas , Catequina/análogos & derivados , Catequina/análisis , Catequina/normas , Cromatografía Líquida de Alta Presión/métodos , Cromatografía Líquida de Alta Presión/normas , Flavonoides/análisis , Flavonoides/normas , Análisis de los Alimentos/métodos , Análisis de los Alimentos/normas , Hesperidina/análisis , Hesperidina/normas , Estándares de Referencia
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...