Asunto(s)
Infecciones por Virus de Epstein-Barr , Herpesvirus Humano 4/metabolismo , Hidroa Vacciniforme , Linfoma de Células T , Neoplasias Cutáneas , Niño , Infecciones por Virus de Epstein-Barr/tratamiento farmacológico , Infecciones por Virus de Epstein-Barr/metabolismo , Infecciones por Virus de Epstein-Barr/patología , Femenino , Humanos , Hidroa Vacciniforme/tratamiento farmacológico , Hidroa Vacciniforme/metabolismo , Hidroa Vacciniforme/patología , Hidroa Vacciniforme/virología , Linfoma de Células T/tratamiento farmacológico , Linfoma de Células T/metabolismo , Linfoma de Células T/patología , Linfoma de Células T/virología , Neoplasias Cutáneas/tratamiento farmacológico , Neoplasias Cutáneas/metabolismo , Neoplasias Cutáneas/patología , Neoplasias Cutáneas/virologíaAsunto(s)
Vesícula/diagnóstico , Varicela/diagnóstico , Herpes Simple/diagnóstico , Herpes Zóster/diagnóstico , Hidroa Vacciniforme/metabolismo , Vesícula/virología , Varicela/virología , ADN Viral , Diagnóstico Diferencial , Herpes Simple/virología , Herpes Zóster/virología , Herpesvirus Humano 3 , Humanos , Funciones de Verosimilitud , Patología Molecular , Reproducibilidad de los Resultados , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sensibilidad y Especificidad , SimplexvirusAsunto(s)
Herpes Simple/patología , Linfoma Cutáneo de Células T/patología , Neoplasias Cutáneas/patología , Piel/patología , Adulto , Biomarcadores de Tumor/análisis , Biopsia , Diagnóstico Diferencial , Femenino , Herpes Simple/virología , Herpesvirus Humano 4/genética , Humanos , Hidroa Vacciniforme/metabolismo , Hidroa Vacciniforme/patología , Inmunohistoquímica , Hibridación in Situ , Linfoma Cutáneo de Células T/química , Linfoma Cutáneo de Células T/virología , Valor Predictivo de las Pruebas , Pronóstico , ARN Viral/análisis , Piel/química , Piel/virología , Neoplasias Cutáneas/química , Neoplasias Cutáneas/virologíaRESUMEN
Hypercholesterolemia has been suggested to have direct negative effects on myocardial function due to increased reactive oxygen species (ROS) generation and increased myocyte death. Mitochondrial permeability transition (MPT) is a significant mediator of cell death, which is enhanced by ROS generation and attenuated by exercise training. The purpose of this study was to investigate the effect of hypercholesterolemia on the MPT response of cardiac mitochondria. We tested the hypothesis that familial hypercholesterolemic (FH) pigs would have an enhanced MPT response and that exercise training could reverse this phenotype. MPT was assessed by mitochondrial swelling in response to 10-100 µM Ca(2+). FH pigs did show an increased MPT response to Ca(2+) that was associated with decreases in the expression of the putative MPT pore components mitochondrial phosphate carrier (PiC) and cyclophilin-D (CypD). FH also caused increased oxidative stress, depicted by increased protein nitrotyrosylation, as well as decreased levels of reduced GSH in cardiac mitochondria. Expression of the mitochondrial antioxidant enzymes manganese superoxide dismutase (MnSOD), thioredoxin-2 (Trx2), and peroxiredoxin-3 (Prx3) was greatly reduced in the FH pigs. In contrast, cytosolic catalase expression and activity were increased. However, chronic exercise training was able to normalize the MPT response in FH pigs, reduce mitochondrial oxidative stress, and return MnSOD, Trx2, Prx3, and catalase expression/activities to normal. We conclude that FH reduces mitochondrial antioxidants, increases mitochondrial oxidative stress, and enhances the MPT response in the porcine myocardium, and that exercise training can reverse these detrimental alterations.
Asunto(s)
Terapia por Ejercicio , Hidroa Vacciniforme/terapia , Mitocondrias Cardíacas/metabolismo , Proteínas de Transporte de Membrana Mitocondrial/metabolismo , Miocardio/metabolismo , Estrés Oxidativo , Animales , Antioxidantes/metabolismo , Calcio/metabolismo , Catalasa/metabolismo , Peptidil-Prolil Isomerasa F , Ciclofilinas/metabolismo , Modelos Animales de Enfermedad , Genotipo , Hidroa Vacciniforme/genética , Hidroa Vacciniforme/metabolismo , Hidroa Vacciniforme/fisiopatología , Masculino , Poro de Transición de la Permeabilidad Mitocondrial , Peroxiredoxina III/metabolismo , Fenotipo , Proteínas de Transporte de Fosfato/metabolismo , Superóxido Dismutasa/metabolismo , Porcinos , Tiorredoxinas/metabolismo , Factores de TiempoRESUMEN
The sunburn response is markedly reduced by dietary fish oil rich in omega-3 polyunsaturated fatty acids. Because prostaglandins mediate the vasodilatation, we examined the effect of fish oil on ultraviolet (UV) B-induced prostaglandin metabolism. In addition we assessed the potential photoprotective effect of fish oil in light-sensitive patients. Thirteen patients with polymorphic light eruption received dietary supplements of fish oil rich in omega-3 polyunsaturated fatty acids for 3 months. At baseline and 3 months, the minimal erythema dose of UVB irradiation was determined, and a graded UVA challenge given to a forearm to assess the threshold dose for papule provocation. Suction blisters were raised on the other forearm, on control skin, and on skin irradiated with four times the minimal erythema dose of UVB 24 h previously, and blister fluid prostaglandin E2 was measured by radioimmunoassay. Following 3 months of fish oil, the mean minimal erythema dose of UVB irradiation increased from 19.8 +/- 2.6 to 33.8 +/- 3.7 mJ/cm2 (mean +/- SEM), p < 0.01. The UVA provocation test was positive in 10 patients at baseline, and after 3 months nine of these showed reduced sensitivity to papule provocation, p < 0.001. Before fish oil, PGE2 increased from 8.6 (SEM 2.1) ng/ml in control skin to 27.2 (11) ng/ml after UVB, p < 0.01. Following 3 months of fish oil, PGE2 decreased to 4.1 (1) and 9.6 (2.4) ng/ml in control and irradiated skin, respectively, p < 0.05. Reduction of UV-induced inflammation by fish oil may be due, at least partially, to lowered prostaglandin E2 levels. The photoprotection against UVA-provocation of a papular response suggests a clinical application for fish oil in polymorphic light eruption.
Asunto(s)
Grasas Insaturadas en la Dieta/uso terapéutico , Dinoprostona/biosíntesis , Ácidos Docosahexaenoicos , Ácido Eicosapentaenoico , Ácidos Grasos Omega-3/uso terapéutico , Aceites de Pescado/uso terapéutico , Hidroa Vacciniforme/prevención & control , Piel/efectos de los fármacos , Quemadura Solar/prevención & control , Rayos Ultravioleta , Adulto , Anciano , Anciano de 80 o más Años , Vesícula/metabolismo , Grasas Insaturadas en la Dieta/farmacología , Combinación de Medicamentos , Ácidos Grasos Omega-3/farmacología , Femenino , Aceites de Pescado/farmacología , Humanos , Hidroa Vacciniforme/metabolismo , Masculino , Persona de Mediana Edad , Piel/metabolismo , Piel/efectos de la radiación , Quemadura Solar/metabolismo , Vasodilatación/efectos de los fármacos , Vasodilatación/fisiologíaRESUMEN
Urocanic acid was measured by means of high-performance liquid chromatography (HPLC) in the stratum corneum of a 3.5-year-old child suffering from hidroa vacciniformia. Compared to ten healthy children of equal age, the urocanic acid content of the stratum corneum was reduced significantly. In addition, there was an elevated level of some amino acids in the urine, particularly that of histidine and methylhistidine, which seems to be a very important finding. Urocanic acid acts as a natural sun protective agent, and the results are discussed with this in mind. It was not possible to elicit hidroa vacciniformia lesions in the patient by UV-A, as described by others.
Asunto(s)
Epidermis/análisis , Hidroa Vacciniforme/metabolismo , Imidazoles/análisis , Ácido Urocánico/análisis , Aminoácidos/orina , Preescolar , Cromatografía Líquida de Alta Presión , Femenino , Histidina/orina , Humanos , Hidroa Vacciniforme/orinaRESUMEN
Disturbances in the kynurenine pathway are present in pellagra and pellagroid syndromes. In 88 photodermatoses, the authors found increased urinary excretion of xanthurenic acid after oral load of tryptophan in a number of light-induced eruptions, i.e. benign summer photodermatosis (40%), polymorphous light eruptions (37%), and persistent light reactions (36%) as well as in patients with acute alcoholic intoxication. These biochemical abnormalities in the kynurenine pathway allow for a pathogenetic approach in some photodermatoses.
Asunto(s)
Ácido Quinurénico/análogos & derivados , Trastornos por Fotosensibilidad/metabolismo , Triptófano/metabolismo , Xanturenatos , Administración Oral , Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Hidroa Vacciniforme/metabolismo , Ácido Quinurénico/orina , Quinurenina/metabolismo , Masculino , Persona de Mediana Edad , Piridoxina/sangre , Triptófano/administración & dosificaciónRESUMEN
The authors described three cases of Bazin's hydroa vacciniforme. Study of the history of the disease enables present nosological conceptions to be better understood. Diagnosis of hydroa vacciniforme is essentially clinical : appearance of the rash, periodicity and spontaneous improvement after puberty are characteristic. Hydroa vacciniforme has to be distinguished from porphyrias and polymorphous light sensitive eruptions. The etiology of this photodermatosis of child is unknown, but associated disturbances in tryptophan metabolism in some cases seem to be linked to a vitamin B6 deficiency.