RESUMEN
The study of microbial hydrocarbons removal is of great importance for the development of future bioremediation strategies. In this study, we evaluated the removal of a gaseous mixture containing toluene, m-xylene, ethylbenzene, cyclohexane, butane, pentane, hexane and heptane in aerated stirred bioreactors inoculated with Rhodococcus erythropolis and operated under non-sterile conditions. For the real-time measurement of hydrocarbons, a novel systematic approach was implemented using Selected-Ion Flow Tube Mass Spectrometry (SIFT-MS). The effect of the carbon source (â¼9.5 ppmv) on (i) the bioreactors' performance (BR1: dosed with only cyclohexane as a single hydrocarbon versus BR2: dosed with a mixture of the 8 hydrocarbons) and (ii) the evolution of microbial communities over time were investigated. The results showed that cyclohexane reached a maximum removal efficiency (RE) of 53% ± 4% in BR1. In BR2, almost complete removal of toluene, m-xylene and ethylbenzene, being the most water-soluble and easy-to-degrade carbon sources, was observed. REs below 32% were obtained for the remaining compounds. By exposing the microbial consortium to only the five most recalcitrant hydrocarbons, REs between 45% ± 5% and 98% ± 1% were reached. In addition, we observed that airborne microorganisms populated the bioreactors and that the type of carbon source influenced the microbial communities developed. The abundance of species belonging to the genus Rhodococcus was below 10% in all bioreactors at the end of the experiments. This work provides fundamental insights to understand the complex behavior of gaseous hydrocarbon mixtures in bioreactors, along with a systematic approach for the development of SIFT-MS methods.
Asunto(s)
Biodegradación Ambiental , Reactores Biológicos , Hidrocarburos , Rhodococcus , Rhodococcus/metabolismo , Reactores Biológicos/microbiología , Hidrocarburos/metabolismo , Carbono/metabolismo , Contaminantes Atmosféricos/metabolismo , Contaminantes Atmosféricos/análisis , Espectrometría de Masas , Tolueno/metabolismo , Xilenos/metabolismo , Butanos/metabolismo , Derivados del Benceno , PentanosRESUMEN
Both soluble phosphorus (P) deficiency and petroleum hydrocarbon contamination represent challenges in soil environments. While phosphate-solubilizing bacteria and hydrocarbon-degrading bacteria have been identified and employed in environmental bioremediation, the bacteria co-adapted to soluble P deficiency and hydrocarbon contamination has rarely been reported. This study explored the ability of Acinetobacter oleivorans S4 (A. oleivorans S4) to solubilize phosphate using n-hexadecane (H), glucose (G), and a mixed carbon source (HG) in tricalcium phosphate (TCP) medium. A. oleivorans S4 exhibited robust growth in H-TCP, releasing 31 mg L-1 of soluble P. Conversely, A. oleivorans S4 barely grew in G-TCP, releasing 654 mg L-1 of soluble P. In HG-TCP, biomass surpassed that in H-TCP, with phosphate release comparable to that in G-TCP. HPLC analysis revealed a small amount of TCA cycle acids in H-TCP and a large amount of gluconate in G-TCP and HG-TCP. Transcriptomic analysis showed elevated expression of genes associated with alkane degradation, P starvation, N utilization, and trehalose synthesis in H-TCP, revealing the molecular co-adaptation mechanism of A. oleivorans S4. Furthermore, the addition of glucose enhanced alkane degradation, P and N utilization, and reduced trehalose synthesis. It indicated that incomplete glucose metabolism may provide energy for other reactions, and the increase in soluble P mediated by gluconate may alleviate oxidative stress. Overall, A. oleivorans S4 proves promising for remediating soluble P-deficient and hydrocarbon-contaminated environments, and glucose stimulates its transformation into a super phosphate-solubilizing bacterium.
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Acinetobacter , Biodegradación Ambiental , Hidrocarburos , Fósforo , Fósforo/deficiencia , Fósforo/metabolismo , Acinetobacter/metabolismo , Hidrocarburos/metabolismo , Contaminantes del Suelo/metabolismo , Alcanos/metabolismoRESUMEN
Ocean oil pollution has a large impact on the environment and the health of living organisms. Bioremediation cleaning strategies are promising eco-friendly alternatives for tackling this problem. Previously, we designed and reported a hydrocarbon (HC) degrading microbial consortium of four marine strains belonging to the species Alloalcanivorax xenomutans, Halopseudomonas aestusnigri, Paenarthrobacter sp., and Pseudomonas aeruginosa. However, the knowledge about the metabolic potential of this bacterial consortium for HC bioremediation is not yet well understood. Here, we analyzed the complete genomes of these marine bacterial strains accompanied by a phylogenetic reconstruction along with 138 bacterial strains. Synteny between complete genomes of the same species or genus, revealed high conservation among strains of the same species, covering over 91% of their genomic sequences. Functional predictions highlighted a high abundance of genes related to HC degradation, which may result in functional redundancy within the consortium; however, unique and complete gene clusters linked to aromatic degradation were found in the four genomes, suggesting substrate specialization. Pangenome gain and loss analysis of genes involved in HC degradation provided insights into the evolutionary history of these capabilities, shedding light on the acquisition and loss of relevant genes related to alkane and aromatic degradation. Our work, including comparative genomic analyses, identification of secondary metabolites, and prediction of HC-degrading genes, enhances our understanding of the functional diversity and ecological roles of these marine bacteria in crude oil-contaminated marine environments and contributes to the applied knowledge of bioremediation.
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Biodegradación Ambiental , Genoma Bacteriano , Genómica , Hidrocarburos , Filogenia , Hidrocarburos/metabolismo , Genómica/métodos , Consorcios Microbianos/genética , Bacterias/genética , Bacterias/metabolismo , Bacterias/clasificación , Agua de Mar/microbiologíaRESUMEN
The oil industry in Khuzestan province (Southwest Iran) is one of the main reasons contributing to the pollution of the environment in this area. TPH, including both aromatic and aliphatic compounds, are important parameters in creating pollution. The present study aimed to investigate the source of soil contamination by TPH in the Ahvaz oil field in 2022. The soil samples were collected from four oil centers (an oil exploitation unit, an oil desalination unit, an oil rig, and a pump oil center). An area outside the oil field was determined as a control area. Ten samples with three replicates were taken from each area according to the standard methods. Aromatic and aliphatic compounds were measured by HPLC and GC methods. The positive matrix factorization (PMF) model and isomeric ratios were used to determine the source apportionment of aromatic compounds in soil samples. The effects range low and effects range median indices were also used to assess the level of ecological risk of petroleum compounds in the soil samples. The results showed that Benzo.b.fluoranthene had the highest concentration with an average of 5667.7 ug/kg in soil samples in the Ahvaz oil field. The highest average was found in samples from the pump oil center area at 7329.48 ug/kg, while the lowest was found in control samples at 1919.4 ug/kg-1. The highest level of aliphatic components was also found in the pump oil center, with a total of 3649 (mg. Kg-1). The results of source apportionment of petroleum compounds in soil samples showed that oil activities accounted for 51.5% of the measured PAHs in soil. 38.3% of other measured compounds had anthropogenic origins, and only 10.1% of these compounds were of biotic origin. The results of the isomeric ratios also indicated the local petroleum and pyrogenic origin of PAH compounds, which is consistent with the PMF results. The analysis of ecological risk indices resulting from the release of PAHs in the environment showed that, except for fluoranthene, other PAHs in the oil exploitation unit area were above the effects range median level (ERM) and at high risk. The results of the study showed that soil pollution by total petroleum hydrocarbons (TPH), both aromatic and aliphatic, is at a high level, and is mainly caused by human activities, particularly oil activities.
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Monitoreo del Ambiente , Contaminación por Petróleo , Petróleo , Contaminantes del Suelo , Suelo , Contaminantes del Suelo/análisis , Petróleo/análisis , Contaminación por Petróleo/análisis , Suelo/química , Irán , Yacimiento de Petróleo y Gas , Hidrocarburos/análisisRESUMEN
Petroleum hydrocarbons (PHCs) can be biodegraded into CO2, and PHC-contaminated aquifers are always deemed as carbon sources. Fortunately, some carbon fixation microorganisms have been found in PHC-contaminated sites. However, most of the studies are related to volatile short-chain PHC, and few studies focus on long-chain PHC-contaminated sites. To reveal the carbon fixation microorganisms in these sites, in the study, a long-chain PHC polluted site in North China was selected. Through hydrochemical and metagenomics analysis, the structure and capacity of carbon fixing microorganisms in the site were revealed. Results showed that there were many kinds of carbon fixed microorganisms that were identified such as Flavobacterium, Pseudomonas. HP/4HB, rTCA, and DC/4HB cycles were dominated carbon fixation pathways. The long-chain PHC were weakly correlated with carbon fixation microorganisms, but it may stimulate the growth of some carbon fixation microorganisms, such as microorganisms involved in rTCA cycle. PRACTITIONER POINTS: The microorganisms with carbon fixation gene exist in the aquifer contaminated by long-chain petroleum hydrocarbon. Microorganisms that have the ability to degrade petroleum also have the ability to carbon fixation. Long-chain petroleum hydrocarbon may promote the growth of carbon fixation microorganisms.
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Ciclo del Carbono , Agua Subterránea , Hidrocarburos , Petróleo , Contaminantes Químicos del Agua , Petróleo/metabolismo , Hidrocarburos/metabolismo , Agua Subterránea/microbiología , Agua Subterránea/química , Contaminantes Químicos del Agua/metabolismo , Contaminantes Químicos del Agua/química , Bacterias/metabolismo , Bacterias/clasificación , Bacterias/genética , Biodegradación Ambiental , ChinaRESUMEN
Guaymas Basin, located in the Gulf of California, is a hydrothermally active marginal basin. Due to steep geothermal gradients and localized heating by sill intrusions, microbial substrates like short-chain fatty acids and hydrocarbons are abiotically produced from sedimentary organic matter at comparatively shallow depths. We analyzed the effect of hydrocarbons on uptake of hydrocarbons by microorganisms via nano-scale secondary ion mass spectrometry (NanoSIMS) and microbial sulfate reduction rates (SRR), using samples from two drill sites sampled by IODP Expedition 385 (U1545C and U1546D). These sites are in close proximity of each other (ca. 1 km) and have very similar sedimentology. Site U1546D experienced the intrusion of a sill that has since then thermally equilibrated with the surrounding sediment. Both sites currently have an identical geothermal gradient, despite their different thermal history. The localized heating by the sill led to thermal cracking of sedimentary organic matter and formation of potentially bioavailable organic substrates. There were low levels of hydrocarbon and nitrogen uptake in some samples from both sites, mostly in surficial samples. Hydrocarbon and methane additions stimulated SRR in near-seafloor samples from Site U1545C, while samples from Site U1546D reacted positively only on methane. Our data indicate the potential of microorganisms to metabolize hydrocarbons even in the deep subsurface of Guaymas Basin.
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Sedimentos Geológicos , Hidrocarburos , Sedimentos Geológicos/microbiología , Hidrocarburos/metabolismo , Bacterias/metabolismo , Bacterias/genética , Sulfatos/metabolismo , Metano/metabolismo , Espectrometría de Masa de Ion Secundario , Agua de Mar/microbiología , Nitrógeno/metabolismoRESUMEN
Rapid expansion in urban areas has engendered a superfluity of municipal solid waste (MSW) stemming from contemporary civilization, encompassing commercial sectors and human undertakings. Kerbside waste, a type of MSW, has the potential for recycling and reuse at the end of its first life cycle, but is often limited to a linear cycle. This study aimed to assess the life cycle costs of different separation and recycling methods for handling kerbside waste. A new life cycle cost model, drawing from the circular economy's value retention process (VRP) model, has been created and applied to assess the continuous recycling of kerbside glass. The study investigates two key separation techniques, kerbside recycling mixed bin recycling (KRMB) kerbside glass recycling separate bin (KGRSB) and analyses their impact on the life cycle cost of the recycling process. Additionally, the research explores two approaches of recycling and downcycling: closed-loop recycling, which pertains to the recycling of glass containers, and open-looped recycling, which involves the use of recycled glass in asphalt. The results showed when use annually collected waste as the functional unit, the KRMB model incurred lower costs compared to the KGRSB model due to its lower production output. However, when evaluated over a 1-ton production of glass container and asphalt, the KGRSB method demonstrated superior cost performance with a 40-50% reduction compared to the KRMB method. The open-loop recycling method (asphalt) incurred a higher cost compared to the closed-loop recycling method due to its larger production volume over a 21-year period.
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Reciclaje , Residuos Sólidos , Administración de Residuos , Reciclaje/métodos , Reciclaje/economía , Residuos Sólidos/análisis , Administración de Residuos/métodos , Administración de Residuos/economía , Vidrio , Eliminación de Residuos/métodos , Eliminación de Residuos/economía , HidrocarburosRESUMEN
AIMS: Microbial enhanced oil recovery (MEOR) is cost-effective and eco-friendly for oil exploitation. Genetically modified biosurfactants-producing high-yield strains are promising for ex-situ MEOR. However, can they survive and produce biosurfactants in petroleum reservoirs for in-situ MEOR? What is their effect on the native bacterial community? METHODS AND RESULTS: A genetically modified indigenous biosurfactants-producing strain Pseudomonas aeruginosa PrhlAB was bioaugmented in simulated reservoir environments. Pseudomonas aeruginosa PrhlAB could stably colonize in simulated reservoirs. Biosurfactants (200 mg l-1) were produced in simulated reservoirs after bio-augmenting strain PrhlAB. The surface tension of fluid was reduced to 32.1 mN m-1. Crude oil was emulsified with an emulsification index of 60.1%. Bio-augmenting strain PrhlAB stimulated the MEOR-related microbial activities. Hydrocarbon-degrading bacteria and biosurfactants-producing bacteria were activated, while the hydrogen sulfide-producing bacteria were inhibited. Bio-augmenting P. aeruginosa PrhlAB reduced the diversity of bacterial community, and gradually simplified the species composition. Bacteria with oil displacement potential became dominant genera, such as Shewanella, Pseudomonas, and Arcobacter. CONCLUSIONS: Culture-based and sequence-based analyses reveal that genetically modified biosurfactants-producing strain P. aeruginosa PrhlAB are promising for in-situ MEOR as well.
Asunto(s)
Petróleo , Pseudomonas aeruginosa , Tensoactivos , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/metabolismo , Petróleo/metabolismo , Tensoactivos/metabolismo , Biodegradación Ambiental , Bacterias/genética , Bacterias/metabolismo , Bacterias/clasificación , Hidrocarburos/metabolismo , MicrobiotaRESUMEN
Oil spills are a frequent perturbation to the marine environment that has rapid and significant impacts on the local microbiome. Previous studies have shown that exposure to synthetic dispersant alone did not enhance heterotrophic microbial activity or oxidation rates of specific hydrocarbon components but increased the abundance of some taxa (e.g., Colwellia). In contrast, exposure to oil, but not dispersants, increased the abundance of other taxa (e.g., Marinobacter) and stimulated hydrocarbon oxidation rates. Here, we advance these findings by interpreting metatranscriptomic data from this experiment to explore how and why specific components of the microbial community responded to distinct organic carbon exposure regimes. Dispersant alone was selected for a unique community and for dominant organisms that reflected treatment- and time-dependent responses. Dispersant amendment also led to diverging functional profiles among the different treatments. Similarly, oil alone was selected for a community that was distinct from treatments amended with dispersants. The presence of oil and dispersants with added nutrients led to substantial differences in microbial responses, likely suggesting increased fitness driven by the presence of additional inorganic nutrients. The oil-only additions led to a marked increase in the expression of phages, prophages, transposable elements, and plasmids (PPTEPs), suggesting that aspects of microbial community response to oil are driven by the "mobilome," potentially through viral-associated regulation of metabolic pathways in ciliates and flagellates that would otherwise throttle the microbial community through grazing.IMPORTANCEMicrocosm experiments simulated the April 2010 Deepwater Horizon oil spill by applying oil and synthetic dispersants (Corexit EC9500A and EC9527A) to deep ocean water samples. The exposure regime revealed severe negative alterations in the treatments' heterotrophic microbial activity and hydrocarbon oxidation rates. We expanded these findings by exploring metatranscriptomic signatures of the microbial communities during the chemical amendments in the microcosm experiments. Here we report how dominant organisms were uniquely associated with treatment- and time-dependent trajectories during the exposure regimes; nutrient availability was a significant factor in driving changes in metatranscriptomic responses. Remarkable signals associated with PPTEPs showed the potential role of mobilome and viral-associated survival responses. These insights underscore the time-dependent environmental perturbations of fragile marine environments under oil and anthropogenic stress.
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Microbiota , Contaminación por Petróleo , Petróleo , Agua de Mar , Tensoactivos , Microbiota/efectos de los fármacos , Agua de Mar/microbiología , Agua de Mar/química , Tensoactivos/metabolismo , Tensoactivos/farmacología , Bacterias/genética , Bacterias/clasificación , Bacterias/metabolismo , Bacterias/efectos de los fármacos , Transcriptoma , Hidrocarburos/metabolismo , Contaminantes Químicos del Agua/metabolismoRESUMEN
Oxidative aging of bitumen is an inevitable and irreversible phenomenon. Exposure to detrimental factors such as sunlight, oxygen, and UV radiations accelerates the aging of bitumen and bituminous pavement. The aging process induces hardening and embrittlement in bitumen, leading to premature pavement failure. Therefore, for constructing sustainable long-lasting pavements anti-aging additives are used. Among the available additives, the use of antioxidants has emerged as a promising solution to mitigate the aging of bitumen. The current review aims to summarise the existing literature for a comprehensive understanding of the effectiveness of these additives as aging inhibitors. It provides an overview of the chemical pathway involved during bitumen oxidation and various quantification techniques to measure the effect of aging. This review also highlights the potential use of antioxidants in bitumen and elaborates on the working mechanism of different types of antioxidants to prevent bitumen aging. Further, the effect of modification in bitumen at micro, macro, and mixture levels are discussed. Additionally, cost analysis and future prospects on the use of antioxidants for bitumen are presented.
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Antioxidantes , Hidrocarburos , Oxidación-Reducción , Materiales de ConstrucciónRESUMEN
There is a global concern with continuous waste production, and efforts are underway to use these wastes in the construction sector. The negative effects of excessive waste production and the use of virgin materials and energy in Hot Mix Asphalt (HMA) construction are challenging and require attention. The right step towards developing resilient road infrastructure is using cold bitumen emulsion mixtures (CBEMs), a cold mix asphalt (CMA). A long-term fix to issues with waste generation and the creation of flexible pavements with HMA can be found by incorporating wastes into CMA. This work reviews the utilization of numerous wastes from different sectors (agricultural, industrial, construction and demolition, and municipal) that are well-known but underutilized and could be used as potential fillers in cold asphalt mixtures. Focus is given to the critical analysis of wastes' physiochemical characteristics and how these affected the effectiveness of mixtures against primary distresses like stability, stiffness, rutting, cracking, fatigue, and moisture susceptibility. Almost all waste materials incorporation reported enhancement in the mechanical performance of CBEM mixes concerning conventional CBEM mixes. The paper sums up the impact on various mechanical properties of CBEM mixes utilizing waste material filler, presents research gaps and a way forward to inspire researchers, and serves as a guideline for using waste materials as fillers in CBEM mixes. This will improve environmental quality and promote sustainability in the paving industry.
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Materiales de Construcción , Hidrocarburos , Residuos Sólidos , EmulsionesRESUMEN
Biostimulation (providing favorable environmental conditions for microbial growth) and bioaugmentation (introducing exogenous microorganisms) are effective approaches in the bioremediation of petroleum-contaminated soil. However, uncertainty remains in the effectiveness of these two approaches in practical application. In this study, we constructed mesocosms using petroleum hydrocarbon-contaminated soil. We compared the effects of adding nutrients, introducing exogenous bacterial degraders, and their combination on remediating petroleum contamination in the soil. Adding nutrients more effectively accelerated total petroleum hydrocarbon (TPH) degradation than other treatments in the initial 60 days' incubation. Despite both approaches stimulating bacterial richness, the community turnover caused by nutrient addition was gentler than bacterial degrader introduction. As TPH concentrations decreased, we observed a succession in microbial communities characterized by a decline in copiotrophic, fast-growing bacterial r-strategists with high rRNA operon (rrn) copy numbers. Ecological network analysis indicated that both nutrient addition and bacterial degrader introduction enhanced the complexity and stability of bacterial networks. Compared to the other treatment, the bacterial network with nutrient addition had more keystone species and a higher proportion of negative associations, factors that may enhance microbial community stability. Our study demonstrated that nutrient addition effectively regulates community succession and ecological interaction to accelerate the soil TPH degradation.
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Bacterias , Biodegradación Ambiental , Petróleo , Microbiología del Suelo , Contaminantes del Suelo , Contaminantes del Suelo/metabolismo , Petróleo/metabolismo , Bacterias/metabolismo , Bacterias/genética , Bacterias/efectos de los fármacos , Hidrocarburos/metabolismo , Nutrientes/metabolismo , Contaminación por PetróleoRESUMEN
Mangrove ecosystems, characterized by high levels of productivity, are susceptible to anthropogenic activities, notably oil pollution arising from diverse origins including spills, transportation, and industrial effluents. Owing to their role in climate regulation and economic significance, there is a growing interest in developing mangrove conservation strategies. In the Arabian Gulf, mangroves stand as the sole naturally occurring green vegetation due to the region's hot and arid climate. However, they have faced persistent oil pollution for decades. This review focuses on global mangrove distribution, with a specific emphasis on Qatar's mangroves. It highlights the ongoing challenges faced by mangroves, particularly in relation to the oil industry, and the impact of oil pollution on these vital ecosystems. It outlines major oil spill incidents worldwide and the diverse hydrocarbon-degrading bacterial communities within polluted areas, elucidating their potential for bioremediation. The use of symbiotic interactions between mangrove plants and bacteria offers a more sustainable, cost-effective and environmentally friendly alternative. However, the success of these bioremediation strategies depends on a deep understanding of the dynamics of bacterial communities, environmental factors and specific nature of the pollutants.
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Bacterias , Biodegradación Ambiental , Hidrocarburos , Contaminación por Petróleo , Rizosfera , Humedales , Hidrocarburos/metabolismo , Bacterias/metabolismo , Contaminantes Químicos del Agua/metabolismo , Contaminantes Químicos del Agua/análisisRESUMEN
Petroleum hydrocarbons (PHCs) residues in commercially important fish and shrimp species from Asia's largest brackish water lagoon, Chilika and their dietary risk factors like Bioaccumulation factor (BAF), Estimated dietary intake (EDI) and Exposure risk index (ERI) were investigated. The PHCs in water samples were found within the range of 2.21 to 9.41 µg/l; while in organisms, PHCs varied from 0.74 to 3.16 µg/g (wet weight). The lowest and highest PHCs concentration was observed in Etroplus suratensis (0.74 ± 0.12; crude fat 0.57 %) and Nematalosa nasus (3.16 ± 0.12; crude fat 6.43 %) respectively. From human health risk view point, the calculated BAF, EDI, ERI were within the prescribed safe limits. Our finding suggests that Nematalosa nasus can be used as biomonitor species for petroleum hydrocarbon contamination status for this ecosystem and also continuous pollution monitoring programs must be conducted by the concerned authorities to safeguard this important aquatic ecosystem.
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Peces , Hidrocarburos , Petróleo , Contaminantes Químicos del Agua , Animales , India , Medición de Riesgo , Contaminantes Químicos del Agua/análisis , Humanos , Hidrocarburos/análisis , Ecosistema , Monitoreo del Ambiente , Monitoreo Biológico , Contaminación por PetróleoRESUMEN
In this study, a microcosm experiment was conducted to investigate the effects of Na2S2O8 preoxidation combined with biostimulation on petroleum-contaminated soil remediation. The response of microbial community during this process was explored using BIOLOG ECO microplate carbon utilization method and 16â¯s rDNA high-throughput sequencing. The results showed that use of 10â¯mg/g Na2S2O8 removed 19.8â¯% of the petroleum hydrocarbons, reduced soil biotoxicity and did not affect soil microbial activity compared to other concentrations. Therefore, sodium persulfate of ca. 10â¯mg/g was used to oxidize petroleum in soil before the biostimulation experiment with organic and inorganic fertilizers. Our finding showed that the content of total petroleum hydrocarbons (TPHs) in soil was reduced by 43.3â¯% in inorganic fertilizer treatment after 60 days. The results of BIOLOG ECO microplate carbon utilization analysis and 16â¯S rDNA high-throughput sequencing further confirmed that biostimulation quickly restored the microbial activities in oxidant treated soil. The main marker bacteria in chemical oxidation combined with biostimulation remediation were Arthrobacter and Paenarthrobacter, and their relative abundances were both significantly negatively correlated with the content of petroleum hydrocarbons in soil.
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Biodegradación Ambiental , Oxidación-Reducción , Petróleo , Microbiología del Suelo , Contaminantes del Suelo , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/análisis , Fertilizantes/análisis , Sulfatos , Hidrocarburos , Compuestos de Sodio/toxicidad , Suelo/química , Arthrobacter , Restauración y Remediación Ambiental/métodos , Bacterias/efectos de los fármacos , Bacterias/genéticaRESUMEN
The weathering process can cause the volatilization of light components in crude oil, leading to the accumulation of total petroleum hydrocarbons (TPH) in weathered oil field soils. These TPH compounds are relatively resistant to biodegradation, posing a significant environmental hazard by contributing to soil degradation. TPH represents a complex mixture of petroleum-based hydrocarbons classified as persistent organic pollutants in soil and groundwater. The release of TPH pollutants into the environment poses serious threats to ecosystems and human health. Currently, various methods are available for TPH-contaminated soil remediation, with bioremediation technology recognized as an environmentally friendly and cost-effective approach. While converting TPH to CO2 is a common remediation method, the complex structures and diverse types of petroleum hydrocarbons (PHs) involved can result in excessive CO2 generation, potentially exacerbating the greenhouse effect. Alternatively, transforming TPH into energy forms like methane through bioremediation, followed by collection and reuse, can reduce greenhouse gas emissions and energy consumption. This process relies on the synergistic interaction between Methanogens archaea and syntrophic bacteria, forming a consortium known as the oil-degrading bacterial consortium. Methanogens produce methane through anaerobic digestion (AD), with hydrogenotrophic methanogens (HTMs) utilizing H2 as an electron donor, playing a crucial role in biomethane production. Candidatus Methanoliparia (Ca. Methanoliparia) was found in the petroleum archaeal community of weathered Oil field in northeast China. Ca. Methanoliparia has demonstrated its independent ability to decompose and produce new energy (biomethane) without symbiosis, contribute to transitioning weathered oil fields towards new energy. Therefore, this review focuses on the principles, mechanisms, and developmental pathways of HTMs during new energy production in the degradation of PHs. It also discusses strategies to enhance TPH degradation and recovery methods.
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Biodegradación Ambiental , Hidrocarburos , Metano , Petróleo , Contaminantes del Suelo , Petróleo/metabolismo , Hidrocarburos/metabolismo , Contaminantes del Suelo/metabolismo , Metano/metabolismo , Yacimiento de Petróleo y Gas/microbiología , Contaminación por Petróleo , Bacterias/metabolismoRESUMEN
Sediment, as the destination of marine pollutants, often bears much more serious petroleum pollution than water. Biochar is increasingly utilized for remediating organic pollutant-laden sediments, yet its long-term impacts on oil-contaminated sediment remain poorly understood. In this study, simulation experiments adding 2.5 wt% biochars (corn straw and wood chips biochar at different pyrolysis temperatures) were conducted. The effects on petroleum hydrocarbon attenuation, enzyme activities, and microbial community structure were systematically investigated. Results showed enhanced degradation of long-chain alkanes in certain biochar-treated groups. Biochar species and PAH characteristics together lead to the PAHs' attenuation, with low-temperature corn straw biochar facilitating the degradation of phenanthrene, fluorene, and chrysene. Initially, biochars reduced polyphenol oxidase activity but increased urease and dehydrogenase activities. However, there was a noticeable rise in polyphenol oxidase activity for a long time. Biochars influenced bacterial community succession and abundance, likely due to nutrient release stimulating microbial activity. The structural equations model (SEM) reveals that DON affected the enzyme activity by changing the microbial community and thus regulated the degradation of PAHs. These findings shed light on biochar's role in bacterial communities and petroleum hydrocarbon degradation over extended periods, potentially enhancing biochar-based remediation for petroleum-contaminated sediments.
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Biodegradación Ambiental , Carbón Orgánico , Sedimentos Geológicos , Petróleo , Hidrocarburos Policíclicos Aromáticos , Carbón Orgánico/química , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiología , Petróleo/metabolismo , Hidrocarburos Policíclicos Aromáticos/química , Hidrocarburos Policíclicos Aromáticos/metabolismo , Bacterias/metabolismo , Bacterias/efectos de los fármacos , Contaminación por Petróleo , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidad , Hidrocarburos/metabolismo , Hidrocarburos/química , Microbiota/efectos de los fármacos , Catecol Oxidasa/metabolismoRESUMEN
To optimally employ Natural Source Zone Depletion (NSZD) and Enhanced Source Zone Depletion (ESZD) at sites impacted by light non-aqueous phase liquids (LNAPL), monitoring strategies are required. Emerging use of subsurface oxidation-reduction potential (ORP) sensors shows promise for tracking redox evolution, which reflects ongoing biogeochemical processes. However, further understanding of how soil redox dynamics relate to subsurface microbial activity and LNAPL degradation pathways is needed. In this work, soil ORP sensors and DNA and RNA sequencing-based microbiome analysis were combined to elucidate NSZD and ESZD (biostimulation via periodic sulfate addition and biosparging) processes in columns containing LNAPL-impacted soils from a former petroleum refinery. Results show expected relationships between continuous soil redox and active microbial communities. Continuous data revealed spatial and temporal detail that informed interpretation of the hydrocarbon biodegradation data. Redox increases were transient for sulfate addition, and sequencing revealed how hydrocarbon concentration and composition impacted microbiome structure and naphthalene degradation. Periodic biosparging did not result in fully aerobic conditions suggesting observed biodegradation improvements could be explained by alternative anaerobic metabolisms (e.g., iron reduction due to air oxidizing reduced iron). Collectively, data suggest combining continuous redox sensing with microbiome analysis provides insights beyond those possible with either monitoring tool alone.
Asunto(s)
Biodegradación Ambiental , Microbiota , Oxidación-Reducción , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/análisis , Suelo/química , Hidrocarburos/metabolismo , Petróleo/metabolismo , Bacterias/metabolismo , Bacterias/genética , Bacterias/clasificación , Sulfatos/metabolismo , Sulfatos/química , Naftalenos/química , Naftalenos/metabolismoRESUMEN
In-situ remediation of total petroleum hydrocarbon (TPH) contaminated soils via Fenton oxidation is a promising approach. However, determining the proper injection amount of H2O2 and Fe source over the Fenton reaction in the complex geological conditions for in-situ TPH soil remediation remains a daunting challenge. Herein, we introduced a practical and novel approach using soft computational models, a multilayer perception artificial neural network (MPLNN), for predicting the TPH removal performance. In this study, we conducted 48 sets of TPH removal experiments using Fenton oxidation to determine the TPH removal performance of a wide range of different ground conditions and generated 336 data points. As a result, a negative Pearson correlation coefficient was obtained in the Fe injection mass and the natural presence of Fe mineral in the soil, indicating that the excess of Fe could significantly retarded the TPH removal performance in the Fenton reaction. In addition, the MPLNN model with 6-6-1 training using Scaled conjugate gradient backpropagation (SCG) with tangent sigmoid as the transfer function demonstrated a high accuracy for TPH removal prediction with the correlation determination of 0.974 and mean square error value of 0.0259. The optimized MPLNN model achieved less than 20% error for predicting TPH removal performance in actual TPH-contaminated soil via Fenton oxidation. Hence, the proposed MPLNN can be useful in improving the Fenton oxidation of TPH removal performance in-situ soil remediation.