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1.
Front Endocrinol (Lausanne) ; 14: 1261298, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37854186

RESUMEN

Experimental studies in animal models of aging such as nematodes, fruit flies or mice have observed that decreased levels of insulin or insulin signaling promotes longevity. In humans, hyperinsulinemia and concomitant insulin resistance are associated with an elevated risk of age-related diseases suggestive of a shortened healthspan. Age-related disorders include neurodegenerative diseases, hypertension, cardiovascular disease, and type 2 diabetes. High ambient insulin concentrations promote increased lipogenesis and fat storage, heightened protein synthesis and accumulation of non-functional polypeptides due to limited turnover capacity. Moreover, there is impaired autophagy activity, and less endothelial NO synthase activity. These changes are associated with mitochondrial dysfunction and oxidative stress. The cellular stress induced by anabolic activity of insulin initiates an adaptive response aiming at maintaining homeostasis, characterized by activation of the transcription factor Nrf2, of AMP activated kinase, and an unfolded protein response. This protective response is more potent in the long-lived human species than in short-lived models of aging research resulting in a stronger pro-aging impact of insulin in nematodes and fruit flies. In humans, resistance to insulin-induced cell stress decreases with age, because of an increase of insulin and insulin resistance levels but less Nrf2 activation. These detrimental changes might be contained by adopting a lifestyle that promotes low insulin/insulin resistance levels and enhances an adaptive response to cellular stress, as observed with dietary restriction or exercise.


Asunto(s)
Envejecimiento , Hiperinsulinismo , Resistencia a la Insulina , Insulina , Animales , Humanos , Ratones , Envejecimiento/fisiología , Diabetes Mellitus Tipo 2/fisiopatología , Hiperinsulinismo/fisiopatología , Insulina/análisis , Insulina/fisiología , Resistencia a la Insulina/fisiología , Factor 2 Relacionado con NF-E2/metabolismo
2.
Nutr Metab Cardiovasc Dis ; 31(12): 3359-3366, 2021 11 29.
Artículo en Inglés | MEDLINE | ID: mdl-34627695

RESUMEN

BACKGROUND AND AIMS: Although hyperinsulinemia and insulin resistance (IR) together cause metabolic diseases, the available evidence fails to link hyperinsulinemia with blood pressure (BP) elevation. To further understand the role of hyperinsulinemia in the pathophysiology of hypertension, we conducted this study to investigate the moderating effect of fasting insulin (FINS) on the association between IR and BP. METHODS AND RESULTS: The health screening data of 72,076 individuals were analyzed for this moderation analysis. IR was indicated by the homeostatic model assessment of insulin resistance (HOMA-IR), triglyceride-glucose index (TyG), and triglyceride to high-density lipoprotein cholesterol ratio (TG/HDLc). In the adjusted model, three IR indicators were considered independent variables; FINS was used as a moderator, and systolic BP (SBP) and diastolic BP (DBP) were used as dependent variables. The regression coefficient of the interaction term between the three IR indicators and FINS was significantly negative in all moderation models. Simple slope tests and the Johnson-Neymann technique also indicated that FINS negatively moderated the association between IR and BP. CONCLUSIONS: This moderation analysis showed that FINS negatively mediated the association between IR and BP, suggesting that hyperinsulinemia may buffer, not reinforce, the effect of IR on hypertension.


Asunto(s)
Presión Sanguínea , Hiperinsulinismo , Resistencia a la Insulina , Presión Sanguínea/fisiología , Humanos , Hiperinsulinismo/fisiopatología , Hipertensión/epidemiología , Resistencia a la Insulina/fisiología
3.
Diabetes Metab Syndr ; 15(6): 102294, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34562871

RESUMEN

Insulin autoimmune syndrome (IAS) or Hirata's disease is a rare cause of hypoglycemia. It is characterized by hyperinsulinemic hypoglycemia, elevated insulin autoantibody titers, no prior exposure to exogenous insulin and no pathological abnormalities of pancreatic islets. Hypoglycemia usually occurs in the post prandial and post absorptive state. Most cases of IAS are self-limiting, with resolution of symptoms within six months to one year. In intractable cases, treatment modalities include low-carbohydrate meals; acarbose; diazoxide; glucocorticoids; immune-suppressants like Azathioprine, cyclophosphamide, mycophenolate mofetil; plasmapheresis and partial pancreatectomy. Rituximab, an anti CD20 monoclonal antibody, was first used in 2016 in a patient with IAS who did not respond to glucocorticoids. Subsequently, there have been three more case reports of IAS where Rituximab was used along with other modalities of treatment. Here, we report the case of a 64-year old Asian Indian woman who presented with recurrent episodes of severe post prandial hypoglycemia and was diagnosed with insulin autoimmune syndrome. She was managed with continuous glucose monitoring and two doses of Rituximab 10 weeks apart, that resulted in resolution of hypoglycemia. This case report underlies the role of Rituximab as a first line agent for treatment of hypoglycemia in IAS.


Asunto(s)
Autoanticuerpos/inmunología , Enfermedades Autoinmunes/tratamiento farmacológico , Automonitorización de la Glucosa Sanguínea/métodos , Glucemia/análisis , Hiperinsulinismo/fisiopatología , Factores Inmunológicos/uso terapéutico , Anticuerpos Insulínicos/inmunología , Rituximab/uso terapéutico , Autoanticuerpos/sangre , Enfermedades Autoinmunes/sangre , Enfermedades Autoinmunes/inmunología , Femenino , Humanos , Anticuerpos Insulínicos/sangre , Persona de Mediana Edad
4.
Diabetologia ; 64(10): 2237-2246, 2021 10.
Artículo en Inglés | MEDLINE | ID: mdl-34272965

RESUMEN

AIMS/HYPOTHESIS: Our aim was to explore metabolic pathways linking overnutrition in utero to development of adiposity in normal-weight children. METHODS: We included 312 normal-weight youth exposed or unexposed to overnutrition in utero (maternal BMI ≥25 kg/m2 or gestational diabetes). Fasting insulin, glucose and body composition were measured at age ~10 years (baseline) and ~16 years (follow-up). We examined associations of overnutrition in utero with baseline fasting insulin, followed by associations of baseline fasting insulin with adiposity (BMI z score [BMIZ], subcutaneous adipose tissue [SAT], visceral adipose tissue [VAT]), insulin resistance (HOMA-IR) and fasting glucose during follow-up. RESULTS: >All participants were normal weight at baseline (BMIZ -0.32 ± 0.88), with no difference in BMIZ for exposed vs unexposed youth (p = 0.14). Of the study population, 47.8% were female sex and 47.4% were of white ethnicity. Overnutrition in utero corresponded with 14% higher baseline fasting insulin (geometric mean ratio 1.14 [95% CI 1.01, 1.29]), even after controlling for VAT/SAT ratio. Higher baseline fasting insulin corresponded with higher BMIZ (0.41 [95% CI 0.26, 0.55]), SAT (13.9 [95% CI 2.4, 25.4] mm2), VAT (2.0 [95% CI 0.1, 3.8] mm2), HOMA-IR (0.87 [95% CI 0.68, 1.07]) and fasting glucose (0.23 [95% CI 0.09, 0.38] SD). CONCLUSIONS/INTERPRETATION: Overnutrition in utero may result in hyperinsulinaemia during childhood, preceding development of adiposity. However, our study started at age 10 years, so earlier metabolic changes in response to overnutrition were not taken into account. Longitudinal studies in normal-weight youth starting earlier in life, and with repeated measurements of body weight, fat distribution, insulin sensitivity, beta cell function and blood glucose levels, are needed to clarify the sequence of metabolic changes linking early-life exposures to adiposity and dysglycaemia.


Asunto(s)
Adiposidad/fisiología , Diabetes Mellitus Tipo 2/fisiopatología , Hiperinsulinismo/fisiopatología , Hipernutrición/fisiopatología , Efectos Tardíos de la Exposición Prenatal/fisiopatología , Adolescente , Glucemia/metabolismo , Composición Corporal , Índice de Masa Corporal , Niño , Femenino , Estudios de Seguimiento , Humanos , Insulina/sangre , Resistencia a la Insulina/fisiología , Grasa Intraabdominal/metabolismo , Masculino , Embarazo
5.
Physiol Res ; 70(4): 509-522, 2021 08 31.
Artículo en Inglés | MEDLINE | ID: mdl-34062073

RESUMEN

A substantial body of literature has provided evidence that type 2 diabetes mellitus (T2DM) and colorectal neoplasia share several common factors. Both diseases are among the leading causes of death worldwide and have an increasing incidence. In addition to usual risk factors such as sedentary lifestyle, obesity, and family history, common pathophysiological mechanisms involved in the development of these diseases have been identified. These include changes in glucose metabolism associated with adipose tissue dysfunction including insulin resistance resulting to hyperinsulinemia and chronic hyperglycemia. In addition to altered glucose metabolism, abdominal obesity has been associated with accented carcinogenesis with chronic subclinical inflammation. An increasing number of studies have recently described the role of the gut microbiota in metabolic diseases including T2DM and the development of colorectal cancer (CRC). Due to the interconnectedness of different pathophysiological processes, it is not entirely clear which factor is crucial in the development of carcinogenesis in patients with T2DM. The aim of this work is to review the current knowledge on the pathophysiological mechanisms of colorectal neoplasia development in individuals with T2DM. Here, we review the potential pathophysiological processes involved in the onset and progression of colorectal neoplasia in patients with T2DM. Uncovering common pathophysiological characteristics is essential for understanding the nature of these diseases and may lead to effective treatment and prevention.


Asunto(s)
Neoplasias Colorrectales/fisiopatología , Diabetes Mellitus Tipo 2/fisiopatología , Adiposidad , Animales , Neoplasias Colorrectales/epidemiología , Neoplasias Colorrectales/microbiología , Diabetes Mellitus Tipo 2/epidemiología , Diabetes Mellitus Tipo 2/microbiología , Disbiosis , Metabolismo Energético , Microbioma Gastrointestinal , Humanos , Hiperglucemia/epidemiología , Hiperglucemia/fisiopatología , Hiperinsulinismo/epidemiología , Hiperinsulinismo/fisiopatología , Incidencia , Resistencia a la Insulina , Obesidad/epidemiología , Obesidad/fisiopatología , Medición de Riesgo , Factores de Riesgo
6.
Cell Rep ; 35(13): 109301, 2021 06 29.
Artículo en Inglés | MEDLINE | ID: mdl-34192532

RESUMEN

Hepatic lipid accumulation is a hallmark of type II diabetes (T2D) associated with hyperinsulinemia, insulin resistance, and hyperphagia. Hepatic synthesis of GABA, catalyzed by GABA-transaminase (GABA-T), is upregulated in obese mice. To assess the role of hepatic GABA production in obesity-induced metabolic and energy dysregulation, we treated mice with two pharmacologic GABA-T inhibitors and knocked down hepatic GABA-T expression using an antisense oligonucleotide. Hepatic GABA-T inhibition and knockdown decreased basal hyperinsulinemia and hyperglycemia and improved glucose intolerance. GABA-T knockdown improved insulin sensitivity assessed by hyperinsulinemic-euglycemic clamps in obese mice. Hepatic GABA-T knockdown also decreased food intake and induced weight loss without altering energy expenditure in obese mice. Data from people with obesity support the notion that hepatic GABA production and transport are associated with serum insulin, homeostatic model assessment for insulin resistance (HOMA-IR), T2D, and BMI. These results support a key role for hepatocyte GABA production in the dysfunctional glucoregulation and feeding behavior associated with obesity.


Asunto(s)
Hiperfagia/metabolismo , Hiperfagia/fisiopatología , Hígado/metabolismo , Hígado/fisiopatología , Obesidad/metabolismo , Obesidad/fisiopatología , Ácido gamma-Aminobutírico/metabolismo , 4-Aminobutirato Transaminasa/metabolismo , Animales , Biomarcadores/metabolismo , Dieta Alta en Grasa , Metabolismo Energético , Conducta Alimentaria , Glucosa/metabolismo , Técnica de Clampeo de la Glucosa , Homeostasis , Humanos , Hiperinsulinismo/complicaciones , Hiperinsulinismo/metabolismo , Hiperinsulinismo/fisiopatología , Hiperfagia/complicaciones , Resistencia a la Insulina , Hígado/inervación , Masculino , Ratones Endogámicos C57BL , Ratones Obesos , Obesidad/complicaciones , Vagotomía , Nervio Vago/fisiopatología
7.
Diab Vasc Dis Res ; 18(2): 14791641211011009, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33908285

RESUMEN

INTRODUCTION: Increasing arterial stiffness is a feature of vascular aging that is accelerated by conditions that enhance cardiovascular risk, including diabetes mellitus. Multiple studies demonstrate divergence of carotid-femoral pulse wave velocity and augmentation index in persons with diabetes mellitus, though mechanisms responsible for this are unclear. MATERIALS AND METHODS: We tested the effect of acutely and independently increasing plasma glucose, plasma insulin, or both on hemodynamic function and markers of arterial stiffness (including carotid-femoral pulse wave velocity, augmentation index, forward and backward wave reflection amplitude, and wave reflection magnitude) in a four-arm, randomized study of healthy young adults. RESULTS: Carotid-femoral pulse wave velocity increased only during hyperglycemic-hyperinsulinemia (+0.36 m/s; p = 0.032), while other markers of arterial stiffness did not change (all p > 0.05). Heart rate (+3.62 bpm; p = 0.009), mean arterial pressure (+4.14 mmHg; p = 0.033), central diastolic blood pressure (+4.16 mmHg; p = 0.038), and peripheral diastolic blood pressure (+4.09 mmHg; p = 0.044) also significantly increased during hyperglycemic-hyperinsulinemia. CONCLUSIONS: Hyperglycemic-hyperinsulinemia acutely increased cfPWV, heart rate, mean arterial pressure, and diastolic blood pressure in healthy humans, perhaps reflecting enhanced sympathetic tone. Whether repeated bouts of hyperglycemia with hyperinsulinemia contribute to chronically-enhanced arterial stiffness remains unknown.


Asunto(s)
Aorta/fisiopatología , Glucemia/metabolismo , Hiperglucemia/fisiopatología , Hiperinsulinismo/fisiopatología , Insulina/sangre , Rigidez Vascular , Adolescente , Adulto , Biomarcadores/sangre , Velocidad de la Onda del Pulso Carotídeo-Femoral , Femenino , Humanos , Hiperglucemia/sangre , Hiperglucemia/diagnóstico , Hiperinsulinismo/sangre , Hiperinsulinismo/diagnóstico , Masculino , Factores de Tiempo , Virginia , Adulto Joven
8.
J Diabetes Res ; 2021: 5123241, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33708999

RESUMEN

Insulin resistance is a state of impaired responsiveness to insulin action. This condition not only results in deficient glucose uptake but increases the risk for cardiovascular diseases (CVD), stroke, and obesity. The present work investigates the molecular mechanisms of high carbohydrate and fat diet in inducing prediabetic hyperinsulinemia and effect of exercise on InsR signaling events, muscular AChE, and lactate dehydrogenase activity. Adult male Wistar rats were divided into the control (C) diet group, high-carbohydrate diet (HCD) group, high-fat diet (HFD) group, and HCD and HFD groups with exercise (HCD Ex and HFD Ex, respectively). Acetyl choline esterase activity, lactate dehydrogenase activity, total lactate levels, IRS1 phosphorylations, and Glut4 expression patterns were studied in the muscle tissue among these groups. High carbohydrate and fat feeding led to hyperinsulinemic status with reduced acetylcholine esterase (AChE) activity and impaired phosphorylation of IRS1 along with increased lactate concentrations in the muscle. Exercise significantly upregulated phosphoinositide 3 kinase (PI3K) docking site phosphorylation and downregulated the negative IRS1 phosphorylations thereby increasing the glucose transporter (GLUT) expressions and reducing the lactate accumulation. Also, the levels of second messengers like IP3 and cAMP were increased with exercise. Increased second messenger levels induce calcium release thereby activating the downstream pathway promoting the translocation of GLUT4 to the plasma membrane. Our results showed that the metabolic and signaling pathway dysregulations seen during diet-induced hyperinsulinemia, a metabolic condition seen during the early stages in the development of prediabetes, were improved with vigorous physical exercise. Thus, exercise can be considered as an excellent management approach over drug therapy for diabetes and its complications.


Asunto(s)
Glucemia/metabolismo , Dieta Alta en Grasa , Carbohidratos de la Dieta , Terapia por Ejercicio , Hiperinsulinismo/terapia , Resistencia a la Insulina , Insulina/sangre , Contracción Muscular , Músculo Esquelético/metabolismo , Condicionamiento Físico Animal , Acetilcolinesterasa/metabolismo , Animales , Modelos Animales de Enfermedad , Proteínas Ligadas a GPI/metabolismo , Transportador de Glucosa de Tipo 4/metabolismo , Hiperinsulinismo/sangre , Hiperinsulinismo/fisiopatología , Proteínas Sustrato del Receptor de Insulina/metabolismo , Masculino , Fosfatidilinositol 3-Quinasa/metabolismo , Fosforilación , Ratas Wistar , Sistemas de Mensajero Secundario
9.
Nat Commun ; 12(1): 565, 2021 01 25.
Artículo en Inglés | MEDLINE | ID: mdl-33495474

RESUMEN

Accumulating evidence indicates that obesity with its associated metabolic dysregulation, including hyperinsulinemia and aberrant circadian rhythms, increases the risk for a variety of cancers including postmenopausal breast cancer. Caloric restriction can ameliorate the harmful metabolic effects of obesity and inhibit cancer progression but is difficult to implement and maintain outside of the clinic. In this study, we aim to test a time-restricted feeding (TRF) approach on mouse models of obesity-driven postmenopausal breast cancer. We show that TRF abrogates the obesity-enhanced mammary tumor growth in two orthotopic models in the absence of calorie restriction or weight loss. TRF also reduces breast cancer metastasis to the lung. Furthermore, TRF delays tumor initiation in a transgenic model of mammary tumorigenesis prior to the onset of obesity. Notably, TRF increases whole-body insulin sensitivity, reduces hyperinsulinemia, restores diurnal gene expression rhythms in the tumor, and attenuates tumor growth and insulin signaling. Importantly, inhibition of insulin secretion with diazoxide mimics TRF whereas artificial elevation of insulin through insulin pumps implantation reverses the effect of TRF, suggesting that TRF acts through modulating hyperinsulinemia. Our data suggest that TRF is likely to be effective in breast cancer prevention and therapy.


Asunto(s)
Neoplasias de la Mama/prevención & control , Modelos Animales de Enfermedad , Ayuno , Hiperinsulinismo/prevención & control , Obesidad/prevención & control , Posmenopausia/fisiología , Animales , Neoplasias de la Mama/sangre , Neoplasias de la Mama/fisiopatología , Restricción Calórica/métodos , Línea Celular Tumoral , Dieta Alta en Grasa , Femenino , Humanos , Hiperinsulinismo/sangre , Hiperinsulinismo/fisiopatología , Resistencia a la Insulina/fisiología , Ratones Endogámicos C57BL , Ratones Obesos , Obesidad/sangre , Obesidad/fisiopatología , Ovariectomía , Posmenopausia/sangre
10.
Am J Physiol Endocrinol Metab ; 319(4): E721-E733, 2020 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-32830555

RESUMEN

The effect of chronic of hyperinsulinemia in the fetal liver is poorly understood. Here, we produced hyperinsulinemia with euglycemia for ∼8 days in fetal sheep [hyperinsulinemic (INS)] at 0.9 gestation. INS fetuses had increased insulin and decreased oxygen and amino acid (AA) concentrations compared with saline-infused fetuses [control (CON)]. Glucose (whole body) utilization rates were increased, as expected, in INS fetuses. In the liver, however, there were few differences in genes and metabolites related to glucose and lipid metabolism and no activation of insulin signaling proteins (Akt and mTOR). There was increased p-AMPK activation and decreased mitochondrial mass (PGC1A expression, mitochondrial DNA content) in INS livers. Using an unbiased multivariate analysis with 162 metabolites, we identified effects on AA and one-carbon metabolism in the INS liver. Expression of the transaminase BCAT2 and glutaminase genes GLS1 and GLS2 was decreased, supporting decreased AA utilization. We further evaluated the roles of hyperinsulinemia and hypoxemia, both present in INS fetuses, on outcomes in the liver. Expression of PGC1A correlated only with hyperinsulinemia, p-AMPK correlated only with hypoxemia, and other genes and metabolites correlated with both hyperinsulinemia and hypoxemia. In fetal hepatocytes, acute treatment with insulin activated p-Akt and decreased PGC1A, whereas hypoxia activated p-AMPK. Overall, chronic hyperinsulinemia produced greater effects on amino acid metabolism compared with glucose and lipid metabolism and a novel effect on one-carbon metabolism in the fetal liver. These hepatic metabolic responses may result from the downregulation of insulin signaling and antagonistic effects of hypoxemia-induced AMPK activation that develop with chronic hyperinsulinemia.


Asunto(s)
Hiperinsulinismo/metabolismo , Insulina/metabolismo , Hígado/fisiopatología , Ovinos/fisiología , Aminoácidos/metabolismo , Animales , Femenino , Feto/metabolismo , Regulación de la Expresión Génica , Glucosa/metabolismo , Hepatocitos/metabolismo , Hiperinsulinismo/fisiopatología , Metabolismo de los Lípidos , Hígado/embriología , Mitocondrias Hepáticas/metabolismo , Consumo de Oxígeno/fisiología , Embarazo , Transducción de Señal
11.
Am J Physiol Endocrinol Metab ; 319(2): E410-E426, 2020 08 01.
Artículo en Inglés | MEDLINE | ID: mdl-32663101

RESUMEN

Diabetes is a chronic, progressive disease that calls for longitudinal data and analysis. We introduce a longitudinal mathematical model that is capable of representing the metabolic state of an individual at any point in time during their progression from normal glucose tolerance to type 2 diabetes (T2D) over a period of years. As an application of the model, we account for the diversity of pathways typically followed, focusing on two extreme alternatives, one that goes through impaired fasting glucose (IFG) first and one that goes through impaired glucose tolerance (IGT) first. These two pathways are widely recognized to stem from distinct metabolic abnormalities in hepatic glucose production and peripheral glucose uptake, respectively. We confirm this but go beyond to show that IFG and IGT lie on a continuum ranging from high hepatic insulin resistance and low peripheral insulin resistance to low hepatic resistance and high peripheral resistance. We show that IFG generally incurs IGT and IGT generally incurs IFG on the way to T2D, highlighting the difference between innate and acquired defects and the need to assess patients early to determine their underlying primary impairment and appropriately target therapy. We also consider other mechanisms, showing that IFG can result from impaired insulin secretion, that non-insulin-dependent glucose uptake can also mediate or interact with these pathways, and that impaired incretin signaling can accelerate T2D progression. We consider whether hyperinsulinemia can cause insulin resistance in addition to being a response to it and suggest that this is a minor effect.


Asunto(s)
Diabetes Mellitus Tipo 2/fisiopatología , Glucemia/análisis , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Diabetes Mellitus Tipo 2/etiología , Progresión de la Enfermedad , Ayuno , Glucosa/biosíntesis , Intolerancia a la Glucosa , Prueba de Tolerancia a la Glucosa , Humanos , Hiperinsulinismo/fisiopatología , Incretinas/metabolismo , Insulina/metabolismo , Resistencia a la Insulina/fisiología , Secreción de Insulina/fisiología , Células Secretoras de Insulina/fisiología , Hígado/metabolismo , Modelos Teóricos , Transducción de Señal/fisiología
12.
Diabetes Care ; 43(9): 2176-2182, 2020 09.
Artículo en Inglés | MEDLINE | ID: mdl-32661106

RESUMEN

OBJECTIVE: To distinguish the effects of hyperglycemia and hyperinsulinemia on exercise-induced increases in Rd and endogenous glucose production (EGP) in type 1 diabetes. RESEARCH DESIGN AND METHODS: We studied six participants without diabetes and six participants with type 1 diabetes on three visits in random order for the following: euglycemia, low insulin (EuLoI); euglycemia, high insulin (EuHiI); and hyperglycemia, low insulin (HyLoI). Glucose fluxes were measured using [6,6-2H2] glucose before, during, and after 60 min of exercise. RESULTS: Rd increased (P < 0.01) with exercise within groups, while peak Rd during exercise was lower (P < 0.01) in participants with type 1 diabetes than participants without diabetes during all visits. In type 1 diabetes participants, EGP increased (P < 0.001) with exercise during EuLoI and HyLoI but not during EuHiI. This demonstrates that hyperinsulinemia, but not hyperglycemia, blunts the compensatory exercise-induced increase in EGP in type 1 diabetes. CONCLUSIONS: The data from this pilot study indicate that 1) exercise-induced compensatory increase in EGP was inhibited in participants with type 1 diabetes with hyperinsulinemia but not with hyperglycemia; 2) in contrast, in participants without diabetes, exercise-induced increase in EGP was inhibited only during combined hyperinsulinemia and hyperglycemia. Taken together, these results suggest that low insulin coupled with euglycemia or modest hyperglycemia appear to be the most favorable milieu for type 1 diabetes during exercise.


Asunto(s)
Diabetes Mellitus Tipo 1/metabolismo , Ejercicio Físico/fisiología , Glucosa/metabolismo , Hiperglucemia/metabolismo , Hiperinsulinismo/metabolismo , Insulina/metabolismo , Adolescente , Adulto , Glucemia/metabolismo , Diabetes Mellitus Tipo 1/complicaciones , Diabetes Mellitus Tipo 1/fisiopatología , Metabolismo Energético/fisiología , Femenino , Técnica de Clampeo de la Glucosa , Humanos , Hiperglucemia/sangre , Hiperglucemia/fisiopatología , Hiperinsulinismo/sangre , Hiperinsulinismo/fisiopatología , Insulina/sangre , Masculino , Proyectos Piloto , Adulto Joven
13.
Am J Physiol Heart Circ Physiol ; 319(1): H162-H170, 2020 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-32502373

RESUMEN

Vasodilatory effects of insulin support the delivery of insulin and glucose to skeletal muscle. Concurrently, insulin exerts central effects that increase sympathetic nervous system activity (SNA), which is required for the acute maintenance of blood pressure (BP). Indeed, in a cohort of young healthy adults, herein we show that intravenous infusion of insulin increases muscle SNA while BP is maintained. We next tested the hypothesis that sympathoexcitation evoked by hyperinsulinemia restrains insulin-stimulated peripheral vasodilation and contributes to sustaining BP. To address this, a separate cohort of participants were subjected to 5-s pulses of neck suction (NS) to simulate carotid hypertension and elicit a reflex-mediated reduction in SNA. NS was conducted before and 60 min following intravenous infusion of insulin. Insulin infusion caused an increase in leg vascular conductance and cardiac output (CO; P < 0.050), with maintenance of BP (P = 0.540). As expected, following NS, decreases in BP were greater in the presence of hyperinsulinemia compared with control (P = 0.045). However, the effect of NS on leg vascular conductance did not differ between insulin and control conditions (P = 0.898). Instead, the greater decreases in BP following NS in the setting of insulin infusion paralleled with greater decreases in CO (P = 0.009). These findings support the idea that during hyperinsulinemia, SNA-mediated increase in CO, rather than restraint of leg vascular conductance, is the principal contributor to the maintenance of BP. Demonstration in isolated arteries that insulin suppresses α-adrenergic vasoconstriction suggests that the observed lack of restraint of leg vascular conductance may be attributed to sympatholytic actions of insulin.NEW & NOTEWORTHY We examined the role of sympathetic activation in restraining vasodilatory responses to hyperinsulinemia and sustaining blood pressure in healthy adults. Data are reported from two separate experimental protocols in humans and one experimental protocol in isolated arteries from mice. Contrary to our hypothesis, the present findings support the idea that during hyperinsulinemia, a sympathetically mediated increase in cardiac output, rather than restraint of peripheral vasodilation, is the principal contributor to the maintenance of systemic blood pressure.


Asunto(s)
Presión Sanguínea , Gasto Cardíaco , Hiperinsulinismo/fisiopatología , Sistema Nervioso Simpático/fisiopatología , Vasodilatación , Adrenérgicos/farmacología , Adulto , Animales , Arterias/efectos de los fármacos , Arterias/fisiología , Femenino , Humanos , Insulina/administración & dosificación , Insulina/farmacología , Pierna/irrigación sanguínea , Masculino , Ratones , Ratones Endogámicos C57BL , Flujo Sanguíneo Regional
14.
Gynecol Endocrinol ; 36(12): 1062-1069, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32544018

RESUMEN

PURPOSE: The aim of this longitudinal, controlled, and retrospective pilot study was to assess how metformin, associated with a contraceptive vaginal ring, may influence lipid and carbohydrate metabolism, and surrogate markers of arterial function in normal weight polycystic ovary syndrome patients. MATERIAL AND METHODS: Among 28 lean patients, 15 were treated with vaginal ring plus metformin and 13 women with only vaginal ring. The effects were assessed after six months. The patients were submitted to evaluation of lipid and carbohydrate metabolism; Doppler analysis of ophthalmic artery; brachial artery flow-mediated vasodilatation; and oral glucose tolerance test. RESULTS: After six months, the fasting insulin, glucose/insulin ratio, and homeostatic model assessment estimates for insulin resistance were significantly improved in metformin group. The ophthalmic artery pulsatility index did not significantly improve in either group. The brachial artery vasodilation was better in metformin treated patients. CONCLUSION: Metformin, associated with vaginal ring, improves the insulin and carbohydrate metabolism. This, associated with the significant improvements of surrogate markers of arterial function, may be responsible of a slight possible cardiovascular and cerebrovascular protective effect.


Asunto(s)
Agentes Anticonceptivos Hormonales/administración & dosificación , Dispositivos Anticonceptivos Femeninos , Desogestrel/administración & dosificación , Etinilestradiol/administración & dosificación , Hiperinsulinismo/tratamiento farmacológico , Hipoglucemiantes/uso terapéutico , Metformina/uso terapéutico , Síndrome del Ovario Poliquístico/tratamiento farmacológico , Adulto , Glucemia/metabolismo , Arteria Braquial/fisiopatología , Femenino , Prueba de Tolerancia a la Glucosa , Humanos , Hiperinsulinismo/metabolismo , Hiperinsulinismo/fisiopatología , Resistencia a la Insulina , Estudios Longitudinales , Arteria Oftálmica/diagnóstico por imagen , Proyectos Piloto , Síndrome del Ovario Poliquístico/metabolismo , Síndrome del Ovario Poliquístico/fisiopatología , Estudios Retrospectivos , Ultrasonografía Doppler , Vasodilatación , Adulto Joven
15.
Nutrients ; 12(6)2020 Jun 22.
Artículo en Inglés | MEDLINE | ID: mdl-32580282

RESUMEN

Diet is a factor which can influence both glycaemic variables and body mass. The aim of this study was to compare the influence of a 12-week, well-planned, low-calorie ketogenic diet (LCKD) on hyperglycaemic, hyperinsulinemic and lipid profile in adult, overweight or obese females. Ninety-one females who participated in the study were divided into two groups: a LCKD group who followed a hypocaloric ketogenic diet (8% of carbohydrate, 72% of fat and 20% of proteins) (n = 46), and a control group (CG) (n = 45) who continued their typical diet (50% of carbohydrates, 32% of fat and 18% of proteins). METHODS: Baseline and post-intervention glucose (Gl), insulin (I), glycated haemoglobin (HbA1c), Homeostatic model assessment HOMA-IR, triglycerides (TG) and high-density cholesterol (HDL-C) were evaluated. Also, body mass (BM), waist circumference (WC), hip circumference (HC) and thigh circumference (TC) were measured. RESULTS: Compared with the CG, there were significant changes observed in the LCKD group regarding all biochemical variables. Also, BM, TC, WC and AC changed significantly in the LCKD group compared with the CG. CONCLUSIONS: The 12-week LCKD intervention changed the glucose control variables, body mass, as well as waist, hip and thigh circumferences. A low-calorie ketogenic diet may be recommended for adult females with glucose control variables disturbance and excess body mass.


Asunto(s)
Restricción Calórica/estadística & datos numéricos , Dieta Cetogénica/estadística & datos numéricos , Control Glucémico/estadística & datos numéricos , Hiperinsulinismo/dietoterapia , Obesidad/dietoterapia , Sobrepeso/dietoterapia , Adulto , Antropometría , Glucemia/análisis , Índice de Masa Corporal , HDL-Colesterol/sangre , Femenino , Hemoglobina Glucada/análisis , Humanos , Hiperinsulinismo/fisiopatología , Persona de Mediana Edad , Obesidad/fisiopatología , Sobrepeso/fisiopatología , Triglicéridos/sangre , Circunferencia de la Cintura
16.
Am J Physiol Regul Integr Comp Physiol ; 319(1): R11-R18, 2020 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-32401628

RESUMEN

Consumption of a single, sugar-sweetened beverage (SSB) impairs vascular endothelial function. Regular aerobic exercise improves endothelium-dependent vasodilation; however, it is unknown whether these beneficial effects persist with frequent SSB consumption. Therefore, the purpose of this study was twofold; we studied the effects of repetitive SSB consumption (75 g d-glucose, 3 times/day) for 1 wk (Glu, n = 13, 23 ± 4 yr, 23.5 ± 3.4 kg/m2) on endothelium-dependent vasodilation (FMD). Then, in a separate cohort, we investigated whether 45 min of moderate-intensity aerobic exercise on five separate days offset the hypothesized decrease in FMD during the Glu protocol (Glu+Ex, n = 11, 21 ± 3 yr, 23.8 ± 2.4 kg/m2). Baseline, fasting [glucose] (P = 0.15), [insulin] (P = 0.25), %FMD (P = 0.48), absolute FMD (P = 0.66), and shear rate area under the curve (SRAUC; P = 0.82) were similar between groups. Following the interventions, fasting [glucose] (Glu: 94 ± 6 to 92 ± 6 mg/dL, Glu+Ex: 89 ± 8 to 87 ± 6 mg/dL, P = 0.74) and [insulin] (Glu: 11.3 ± 6.2 to 11.8 ± 8.9 µU/mL, Glu+Ex: 8.7 ± 2.9 to 9.4 ± 3.2 µU/mL, P = 0.89) were unchanged. %FMD was reduced in Glu (6.1 ± 2.2 to 5.1 ± 1.3%) and increased in Glu+Ex (6.6 ± 2.2 to 7.8 ± 2.4%, P < 0.05 for both). SRAUC increased similarly in both Glu [17,715 ± 8,275 to 22,922 ± 4,808 arbitrary units (A.U.)] and Glu+Ex (18,216 ± 4,516 to 21,666 ± 5,392 A.U., main effect of time P < 0.05). When %FMD was adjusted for SRAUC, attenuation was observed in Glu (0.41 ± 0.18 to 0.23 ± 0.08%/s × 103, P < 0.05) but not Glu+Ex (0.38 ± 0.14 to 0.38 ± 0.13%/s × 103, P = 0.88). Despite unchanged fasting [glucose] and [insulin], repeated consumption of SSBs impaired conduit artery vascular endothelial function. Additionally, subjects who engaged in regular moderate-intensity aerobic exercise did not demonstrate the same SSB-induced endothelial dysfunction. Collectively, these data suggest aerobic exercise may offset the deleterious effects of repetitive SSB consumption.


Asunto(s)
Endotelio Vascular/fisiología , Ejercicio Físico/fisiología , Bebidas Azucaradas/efectos adversos , Adolescente , Adulto , Glucemia/análisis , Estudios de Cohortes , Dieta , Humanos , Hiperglucemia/inducido químicamente , Hiperglucemia/fisiopatología , Hiperinsulinismo/inducido químicamente , Hiperinsulinismo/fisiopatología , Insulina/sangre , Masculino , Vasodilatación/efectos de los fármacos , Adulto Joven
17.
Diabetologia ; 63(8): 1564-1575, 2020 08.
Artículo en Inglés | MEDLINE | ID: mdl-32424539

RESUMEN

AIMS/HYPOTHESIS: Islet vascular fibrosis may play an important role in the progression of type 2 diabetes, but there are no mouse models allowing detailed mechanistic studies to understand how a dysfunctional islet microvasculature contributes to diabetes pathogenesis. Here we report that the transgenic AktTg mouse, unlike other mouse strains, shows an increased deposition of extracellular matrix (ECM) proteins in perivascular regions, allowing us to study the cellular mechanisms that lead to islet vascular fibrosis. METHODS: Using immunohistochemistry, we labelled the islet microvasculature and ECM in pancreas sections of AktTg mice and human donors and performed lineage tracing to follow the fate of islet pericytes. We compared islet microvascular responses in living pancreas slices from wild-type and AktTg mice. RESULTS: We found that vascular pericytes proliferate extensively, convert into profibrotic myofibroblasts and substantially contribute to vascular fibrosis in the AktTg mouse model. The increased deposition of collagen I, fibronectin and periostin within the islet is associated with diminished islet perfusion as well as impaired capillary responses to noradrenaline (norepinephrine) and to high glucose in living pancreas slices. CONCLUSIONS/INTERPRETATION: Our study thus illustrates how the AktTg mouse serves to elucidate a cellular mechanism in the development of islet vascular fibrosis, namely a change in pericyte phenotype that leads to vascular dysfunction. Because beta cells in the AktTg mouse are more numerous and larger, and secrete more insulin, in future studies we will test the role beta cell secretory products play in determining the phenotype of pericytes and other cells residing in the islet microenvironment under physiological and pathophysiological conditions. Graphical abstract.


Asunto(s)
Proliferación Celular/fisiología , Hiperinsulinismo/fisiopatología , Miofibroblastos/fisiología , Animales , Matriz Extracelular/metabolismo , Matriz Extracelular/fisiología , Fibrosis/metabolismo , Fibrosis/fisiopatología , Hiperinsulinismo/metabolismo , Inmunohistoquímica , Islotes Pancreáticos/metabolismo , Ratones , Miofibroblastos/metabolismo , Pericitos/metabolismo , Pericitos/fisiología
19.
Can J Cardiol ; 36(5): 671-682, 2020 05.
Artículo en Inglés | MEDLINE | ID: mdl-32389340

RESUMEN

Hyperinsulinemia and insulin resistance were proposed more than 30 years ago to be important contributors to elevated blood pressure (BP) associated with obesity and the metabolic syndrome, also called syndrome X. Support for this concept initially came from clinical and population studies showing correlations among hyperinsulinemia, insulin resistance, and elevated BP in individuals with metabolic syndrome. Short-term studies in experimental animals and in humans provided additional evidence that hyperinsulinemia may evoke increases in sympathetic nervous system (SNS) activity and renal sodium retention that, if sustained, could increase BP. Although insulin infusions may increase SNS activity and modestly raise BP in rodents, chronic insulin administration does not significantly increase BP in lean or obese insulin-resistant rabbits, dogs, horses, or humans. Multiple studies in humans and experimental animals have also shown that severe insulin resistance and hyperinsulinemia may occur in the absence of elevated BP. These observations question whether insulin resistance and hyperinsulinemia are major factors linking obesity/metabolic syndrome with hypertension. Other mechanisms, such as physical compression of the kidneys, activation of the renin-angiotensin-aldosterone system, hyperleptinemia, stimulation of the brain melanocortin system, and SNS activation, appear to play a more critical role in initiating hypertension in obese subjects with metabolic syndrome. However, the metabolic effects of insulin resistance, including hyperglycemia and dyslipidemia, appear to interact synergistically with increased BP to cause vascular and kidney injury that can exacerbate the hypertension and associated injury to the kidneys and cardiovascular system.


Asunto(s)
Hiperinsulinismo/fisiopatología , Hipertensión/fisiopatología , Resistencia a la Insulina/fisiología , Síndrome Metabólico/fisiopatología , Animales , Humanos , Riñón/metabolismo , Leptina/sangre , Obesidad/fisiopatología , Sistema Renina-Angiotensina/fisiología , Sodio/orina , Sistema Nervioso Simpático/fisiología
20.
Clin Exp Hypertens ; 42(7): 614-621, 2020 Oct 02.
Artículo en Inglés | MEDLINE | ID: mdl-32349626

RESUMEN

OBJECTIVE: To investigate if insulin resistance per se or the accompanying hyperinsulinemia induced hypertension and its underlying mechanisms. METHODS: Sprague-Dawley rats were randomized into normal diet-fed group (ND group) and high-fat diet-fed group (HFD group). Then, the HFD group was further randomly divided into the control group (HFD_C group), the PIO group (treated with pioglitazone), the STZ_DM group (to induce diabetes with streptozotocin) and the DM+Ins group (streptozotocin injection followed by insulin treatment). Insulin sensitivity, plasma insulin, endothelin-1, norepinephrine, aldosterone, angiotensinⅡ and 24-h urinary sodium excretion (USE) levels of the groups were measured and analyzed. A multiple stepwise regression analysis method was applied to exam our hypothesis. RESULTS: Compared to HFD_C group, the groups with lower plasma insulin, the PIO group and STZ_DM group, showed higher USE and lower blood pressure. The groups with higher plasma insulin (but same level of insulin resistance), the HFD_C group and DM+Ins group, showed lower USE and higher blood pressure. The 24-h urinary sodium excretion was the most important contributor to the significant changes of blood pressure with an R2 of 25.2% in this animal experiment. CONCLUSIONS: It is the compensatory hyperinsulinemia rather than insulin resistance per se that causes blood pressure elevation. The urinary sodium excretion is the key mediator among the multiple mechanisms. Therapies targeting hyperinsulinemia and restricting salt intake may favor a better control of hypertension associated with insulin resistance.


Asunto(s)
Presión Sanguínea , Diabetes Mellitus Experimental/fisiopatología , Hiperinsulinismo/fisiopatología , Resistencia a la Insulina/fisiología , Insulina/sangre , Sodio/orina , Animales , Glucemia/metabolismo , Presión Sanguínea/efectos de los fármacos , Dieta Alta en Grasa , Hiperinsulinismo/etiología , Hipertensión/complicaciones , Hipoglucemiantes/farmacología , Insulina/farmacología , Masculino , Pioglitazona/farmacología , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley
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