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2.
Mol Immunol ; 128: 10-21, 2020 12.
Artículo en Inglés | MEDLINE | ID: mdl-33045539

RESUMEN

The production of specific antibodies able to recognize allergens from different sources or block interactions between allergens and antibodies mediating allergic reactions is crucial for developing successful tools for diagnostics and therapeutics. Panallergens are highly conserved proteins present in widely different species, implicated in relevant cross-reactions. The panallergen latex profilin (Hev b 8) has been associated with the latex-food-pollen syndrome. We generated five monoclonal IgGs and one IgE from murine hybridomas against recombinant Hev b 8 and evaluated their interaction with this allergen using ELISA and biolayer interferometry (BLI). Affinity purified mAbs exhibited high binding affinities towards rHev b 8, with KD1 values ranging from 10-10 M to 10-11 M. Some of these antibodies also recognized the recombinant profilins from maize and tomato (Zea m 12 and Sola l 1), and the ash tree pollen (Fra e 2). Competition ELISA demonstrated that some mAb pairs could bind simultaneously to rHev b 8. Using BLI, we detected competitive, non-competitive, and partial-competition interactions between pairs of mAbs with rHev b 8, suggesting the existence of at least two non-overlapping epitopes on the surface of this allergen. Three-dimensional models of the Fv of 1B4 and 2D10 IgGs and docking simulations of these Fvs with rHev b 8 revealed these epitopes. Furthermore, these two mAbs inhibited the interaction of polyclonal IgE and IgG4 antibodies from profilin-allergic patients with rHev b 8, indicating that the mAbs and the antibodies present in sera from allergic patients bind to overlapping epitopes on the allergen. These mAbs can be useful tools for immune-localization studies, immunoassay development, or standardization of allergenic products.


Asunto(s)
Anticuerpos Monoclonales/inmunología , Antígenos de Plantas/inmunología , Reacciones Cruzadas/inmunología , Epítopos/inmunología , Látex/inmunología , Profilinas/inmunología , Alérgenos/inmunología , Secuencia de Aminoácidos , Animales , Inmunoglobulina E/inmunología , Inmunoglobulina G/inmunología , Hipersensibilidad al Látex/inmunología , Ratones , Ratones Endogámicos BALB C , Proteínas de Plantas/inmunología , Polen/inmunología
3.
Pharmacology ; 105(11-12): 719-722, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32894843

RESUMEN

Adverse reactions to natural rubber latex (NRL) represent a group of clinical manifestations that include non-allergic reactions and immediate-type or delayed-type allergic reactions. NRL sublingual immunotherapy (SLIT) has been demonstrated to be an effective and safe practice for latex clinical manifestations with good patient tolerance. A pediatric case of NRL allergy managed with an effective tailored SLIT is described. This case is compelling because the girl, who did not have an atopic background, suffered from NRL allergy with high reactivity and unique monosensitization. To the best of our knowledge, this is the first case with this characteristic described to date. Moreover, the SLIT follow-up time reported was unusually long, extending from childhood to adulthood. The case described highlights several problems of real-life management, and it demonstrates how the pediatric allergist plays a key role in the management of all these issues in order to succeed in guiding the patient through the immunotherapy process with a personalized approach, in line with the precision medicine principles. However, further long-term clinical studies are needed to better define the natural history of NRL allergy and find new potential biomarkers of response to NRL immunotherapy.


Asunto(s)
Hipersensibilidad al Látex/tratamiento farmacológico , Inmunoterapia Sublingual/métodos , Niño , Desensibilización Inmunológica/efectos adversos , Desensibilización Inmunológica/métodos , Femenino , Estudios de Seguimiento , Humanos , Inmunoglobulina E/sangre , Hipersensibilidad al Látex/sangre , Hipersensibilidad al Látex/inmunología , Medicina de Precisión/métodos , Goma/efectos adversos , Pruebas Cutáneas , Inmunoterapia Sublingual/efectos adversos
4.
Expert Rev Mol Diagn ; 20(4): 367-373, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32056456

RESUMEN

Introduction: IgE-mediated Hevea latex allergy and associated food-allergies constitute a significant health issue with serious consequences of diagnostic error. Hence, there is a need for more reliable confirmatory diagnostics.Areas covered: Here, we summarize the major limitations of conventional tests using native extracts and describe how piecing together the IgE reactivity profile can benefit correct diagnosis in difficult cases in whom conventional tests yield equivocal or negative results. A diagnostic algorithm integrating traditional sIgE and component-resolved diagnosis (CRD) is presented.Expert opinion: Moreover, it is clear that the discoveries in the field of the Hevea latex proteome will contribute to our understandings and accurate approach of sometimes complex cross-reactivity phenomena that extend beyond the 'latex-fruit syndrome.'


Asunto(s)
Alérgenos/inmunología , Hevea/efectos adversos , Inmunoglobulina E/inmunología , Hipersensibilidad al Látex/diagnóstico , Hipersensibilidad al Látex/inmunología , Goma/efectos adversos , Biomarcadores , Reacciones Cruzadas , Susceptibilidad a Enfermedades , Femenino , Humanos , Inmunoglobulina E/sangre , Hipersensibilidad al Látex/metabolismo , Masculino , Proteoma , Proteómica/métodos
5.
Curr Opin Allergy Clin Immunol ; 20(2): 112-116, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-31850921

RESUMEN

PURPOSE OF REVIEW: During the 1980s, natural rubber latex (NRL) emerged as a major cause of occupational allergy and asthma, especially in healthcare workers using powdered NRL gloves. Increased recognition of NRL allergies and transfer of research findings into prevention strategies resulted into an almost elimination of this health problem. Here, the current knowledge on allergens, their use in diagnostics, prevalence data of occupational NRL allergy and the status of prevention strategy are summarized. RECENT FINDINGS: While the current studies conducted in high-income countries show that prevention measures have reduced NRL allergy, this does not yet apply worldwide. For latex allergy diagnosis, it is important that recombinant allergens as reliable tools are available, because in several European countries and the United States, NRL skin prick test extracts and powdered latex gloves for bronchial challenge test are no longer commercially available, leading to a deficit in diagnostic tools. Due to the fact that latex is suitable with good properties as a raw material for gloves, alternatives with significantly lower protein content and without cross-reactive structures to latex proteins are sought. SUMMARY: Awareness of the sensitization risk associated with the use of latex gloves should still be present in all occupational settings.


Asunto(s)
Alérgenos/inmunología , Carga Global de Enfermedades , Hipersensibilidad al Látex/epidemiología , Enfermedades Profesionales/epidemiología , Goma/efectos adversos , Alérgenos/efectos adversos , Reacciones Cruzadas , Guantes Protectores/efectos adversos , Personal de Salud/estadística & datos numéricos , Humanos , Hipersensibilidad al Látex/diagnóstico , Hipersensibilidad al Látex/inmunología , Hipersensibilidad al Látex/prevención & control , Enfermedades Profesionales/diagnóstico , Enfermedades Profesionales/inmunología , Enfermedades Profesionales/prevención & control , Exposición Profesional/efectos adversos , Exposición Profesional/prevención & control , Prevalencia , Pruebas Cutáneas
6.
Mol Immunol ; 112: 347-357, 2019 08.
Artículo en Inglés | MEDLINE | ID: mdl-31254775

RESUMEN

Peach and apricot can cause allergic reactions with symptoms ranging from mild to very severe, including anaphylaxis. Sometimes subjects allergic to fruits of the Prunus genus have been reported to be also allergic to rubber latex products. The objective of this study is the characterization of a newly identified peach and apricot protein showing similarities with the allergens Hev b 5 from rubber latex and Man e 5 from manioc. This protein has been named ENEA on the basis of the single letter amino acid code of the first four N-terminal residues of the isolated molecule. It has been found in very variable amounts in different peach cultivars and batches. ENEA was isolated from peach pulp extracts by chromatographic separations and identified by direct protein sequencing. At that time, the full length sequence was available only for the homologous protein of the taxonomically closely related apricot, which was produced as a recombinant molecule in Escherichia coli. The following availability of the full length sequence of peach ENEA revealed a very high identity (97%) with the apricot homolog. Similarly to Hev b 5 and to Man e 5, the structural characterization indicated that ENEA is an intrinsically disordered protein. The immunological properties, investigated by dot blotting, the ABA system and the FABER test, showed that ENEA is recognized by specific IgE of allergic patients. In a selected population of 31 patients reporting allergic reactions to peach fruit and/or IgE positive to Hev b 5, 28 and 27 subjects resulted co-sensitized to rENEA and Hev b 5 in the ABA and ISAC test, respectively. In a random population of 3305 suspected allergic patients, analyzed with the FABER test, 17 of them were sensitized to rENEA and 10 of them were also positive to Hev b 5. In addition, both the natural molecule from peach and the recombinant protein of apricot partially inhibited the IgE binding to Hev b 5. In conclusion, a new peach and apricot IgE-binding protein, cross-reacting with the major latex allergen Hev b 5, has been identified. Its variable concentration in the fruit might explain some occasionally occurring allergic reactions. The apricot molecule has recently been registered by the WHO/IUIS Allergen Nomenclature Sub-Committee with the allergen name Pru ar 5. The recombinant form of apricot ENEA, now available, will contribute to allergy diagnosis.


Asunto(s)
Antígenos de Plantas/inmunología , Reacciones Cruzadas/inmunología , Hipersensibilidad al Látex/inmunología , Látex/inmunología , Proteínas de Plantas/inmunología , Prunus armeniaca/inmunología , Prunus persica/inmunología , Adulto , Anciano , Alérgenos/inmunología , Niño , Femenino , Galectina 3/inmunología , Humanos , Inmunoglobulina E/inmunología , Masculino , Persona de Mediana Edad , Prunus/inmunología , Proteínas Recombinantes/inmunología , Adulto Joven
7.
Occup Environ Med ; 76(4): 269-278, 2019 04.
Artículo en Inglés | MEDLINE | ID: mdl-30804164

RESUMEN

OBJECTIVES: To determine the test performance parameters for the retrievable range of high-molecular-weight (HMW) and low-molecular-weight (LMW) occupational allergens and to evaluate the impact of allergenic components and the implementation of measures for test validation. METHODS: A protocol with predefined objectives and inclusion criteria was the basis of an electronic literature search of MEDLINE and EMBASE (time period 1967-2016). The specific inhalation challenge and serial peak flow measurements were the reference standards for the specific IgE (sIgE) test parameters. All of the review procedures were reported according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses. RESULTS: Seventy-one studies were selected, and 62 entered meta-analysis. Pooled pairs analysis indicated a sensitivity of 0.74(95% CI 0.66 to 0.80) and specificity of 0.71(95% CI 0.63 to 0.77) for HMW allergens and a sensitivity of 0.28(95% CI 0.18 to 0.40) and specificity of 0.89(95% CI 0.77 to 0.95) for LMW allergens. Component-specific analysis improved the test parameters for some allergens. Test validation was handled heterogeneously among studies. CONCLUSION: sIgE test performance is rather satisfactory for a wide range of HMW allergens with the potential for component-specific approaches, whereas sensitivity for LMW allergens is considerably lower, indicating methodological complications and/or divergent pathomechanisms. A common standard for test validation is needed.


Asunto(s)
Alérgenos/inmunología , Asma Ocupacional/sangre , Asma Ocupacional/diagnóstico , Inmunoglobulina E/análisis , Alérgenos/metabolismo , Animales , Área Bajo la Curva , Biomarcadores/análisis , Bovinos , Grano Comestible/inmunología , Grano Comestible/metabolismo , Humanos , Inmunoglobulina E/sangre , Hipersensibilidad al Látex/diagnóstico , Hipersensibilidad al Látex/inmunología , Curva ROC
8.
Int J Occup Saf Ergon ; 25(3): 423-428, 2019 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28980879

RESUMEN

Purpose. To determine the prevalence of undesirable skin reactions to latex in dental professionals and students of the School of Dental Medicine in Zagreb, Croatia. Methods. Our research included 444 participants, of which 200 agreed to undergo a skin prick test (SPT). All participants answered a questionnaire in which we asked about incidence of skin lesions, duration of occupational exposure to latex, localization of skin lesions and symptoms. Statistical analysis of the questionnaire and test results was then carried out. Results. Of the total 444 participants surveyed, 249 (56.1%) reported lesions on their skin (professionals 64.8%, students 6.1-58.5%). From the questionnaire, 239 (96.0%) respondents reported lesions on the hands and fingers, mostly in the form of erythema (37.0%) and occasional dryness of skin (29.0%). Positive SPT results were found in 14 (7.0%) out of the 200 respondents who underwent the test. Conclusions. While a large number of subjects (56.1%) reported skin lesions when using latex products at their workplace, the SPT test was positive only in 7.0%. The results show that the prevalence of self-reported skin lesions was significantly related to the length of occupational exposure, with a substantial effect size (p < 0.001; V = 0.334).


Asunto(s)
Dermatitis Profesional/epidemiología , Hipersensibilidad al Látex/epidemiología , Látex/inmunología , Exposición Profesional , Croacia/epidemiología , Personal de Odontología , Odontólogos , Dermatitis Profesional/inmunología , Femenino , Humanos , Látex/efectos adversos , Hipersensibilidad al Látex/inmunología , Masculino , Prevalencia , Pruebas Cutáneas , Estudiantes de Odontología , Encuestas y Cuestionarios
9.
Clin Rev Allergy Immunol ; 56(3): 269-277, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28653246

RESUMEN

The exact prevalence of hypersensitivity reactions related to sexual behaviours is not known; however, they heavily impact on the quality of life and of sex life of affected patients. In fact, not only common respiratory and skin allergies, such as asthma, rhinitis, urticaria and atopic dermatitis, but also food and drug allergy have been found to negatively affect the quality of sex life. Allergic diseases impact on the sexual function in both physical and psychological ways, representing one of the main complaints of a considerable proportion of patients. Sexual behaviours may act as the triggers of allergic reactions or as the carriers of allergens. Food and drug allergens can be carried through human organic fluids, like saliva and semen. Latex in condoms and numerous substances in lubricants, spermicides, topical medications and cosmetics can cause allergic reactions or contact dermatitis. Sexual activity itself is also a potential trigger of symptoms in patients affected by respiratory allergies, like honeymoon asthma and rhinitis. In seminal plasma hypersensitivity, seminal fluid proteins are the culprit allergens. The present review aims at summarizing the state of the art about allergy and sexual behaviours. In clinical practice, the influence of common allergic diseases on the sexual quality of life should be taken carefully into account. Sexual behaviours need to be accounted in the differential diagnosis of hypersensitivity reactions, and awareness on those exposure routes should be raised between different specialists and general practitioners.


Asunto(s)
Asma/inmunología , Dermatitis Alérgica por Contacto/inmunología , Dermatitis Atópica/inmunología , Hipersensibilidad a las Drogas/inmunología , Hipersensibilidad a los Alimentos/inmunología , Rinitis Alérgica/inmunología , Conducta Sexual/fisiología , Asma/diagnóstico , Condones/efectos adversos , Reacciones Cruzadas , Dermatitis Alérgica por Contacto/diagnóstico , Dermatitis Atópica/diagnóstico , Diagnóstico Diferencial , Hipersensibilidad a las Drogas/diagnóstico , Femenino , Hipersensibilidad a los Alimentos/diagnóstico , Humanos , Hipersensibilidad al Látex/diagnóstico , Hipersensibilidad al Látex/inmunología , Masculino , Calidad de Vida , Rinitis Alérgica/diagnóstico , Saliva/inmunología , Semen/inmunología
10.
Int Arch Allergy Immunol ; 178(1): 83-88, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30212836

RESUMEN

BACKGROUND: Natural rubber latex (NRL) allergy is commonly diagnosed according to medical history, skin allergy tests, and serological analyses. However, skin tests are increasingly being abandoned because of (i) their time-consuming nature, (ii) latex preparations for skin tests being not commercially available, and (iii) the use of in-house prepared test solutions is becoming ever more difficult due to increasing regulatory hurdles. In this light, we have evaluated differences in the profiles of current and former patients with suspected latex allergy. METHODS: Sera of skin test-positive patients from a historic cohort (1995-2001, n = 149 patients) and currently (2014-2015, n = 48 patients) were simultaneously analyzed for specific IgE to latex by ImmunoCAP. If the serological screening was positive (≥0.35 kU/L), component-resolved diagnostics including profilins and cross-reactive carbohydrate determinants (CCDs) were performed. RESULTS: In contrast to 88% (131/149) of the skin test-positive patients from the 1990s, only 51.1% (24/47) of the current cohort were found positive for specific IgE to latex. While 48.3% (72/149) of the patients had a convincing positive history in the 1990s, current skin test-positive patients rarely reported a relevant medical history (8.5%, 4/47). Specific IgE levels to latex were significantly higher in former patients with suspected latex allergy (p < 0.001) than in former sensitized individuals without allergy. However, this significant difference was lost in current allergic and sensitized patients with positive skin tests. CONCLUSION: Sensitization profiles in patients with latex allergy have changed significantly over the last 2 decades. Discrimination between NRL sensitization and clinical allergy remains a diagnostic challenge. Our data highlight the need for a combination of all 3 criteria, i.e., patient history, skin test, and analysis of specific IgE, for a correct diagnosis of latex allergy.


Asunto(s)
Alérgenos/inmunología , Hipersensibilidad al Látex/epidemiología , Hipersensibilidad al Látex/inmunología , Látex/efectos adversos , Especificidad de Anticuerpos/inmunología , Reacciones Cruzadas/inmunología , Femenino , Humanos , Inmunización , Inmunoglobulina E/inmunología , Hipersensibilidad al Látex/diagnóstico , Masculino , Prevalencia , Pruebas Cutáneas
11.
J Med Invest ; 65(3.4): 292-295, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30282877

RESUMEN

Natural rubber latex (NRL) allergy is one of the most important causes of severe anaphylaxis during medical intervention. We report a pediatric case of latex allergy with multiple surgical histories. A 12-year-old girl developed anaphylactic shock during the pyeloplasty for ureteropelvic junction restenosis. Latex gloves or medications used during the surgery were suspected to be the cause of anaphylactic shock. We diagnosed her latex allergy on the basis of the results that serum latex-specific IgE, skin prick tests of extract from NRL gloves and recombinant Hev b 6.02 solution were positive. Basophil activation test of NRL gloves was also positive, supporting the diagnosis of immediate allergic reactions caused by NRL. It was speculated that a history of multiple surgeries in infancy became a trigger of sensitization to latex in this patient. Reoperation after the diagnosis of NRL allergy was carried out in a latex-free environment and completed without any allergic symptoms. It would be necessary to perform the pre-screening of latex allergy to prevent the onset of latex allergy especially in the patients with multiple surgical histories. J. Med. Invest. 65:292-295, August, 2018.


Asunto(s)
Anafilaxia/etiología , Complicaciones Intraoperatorias/etiología , Hipersensibilidad al Látex/etiología , Niño , Femenino , Humanos , Inmunoglobulina E/sangre , Hipersensibilidad al Látex/diagnóstico , Hipersensibilidad al Látex/inmunología , Nefrotomía/efectos adversos , Pruebas Cutáneas
13.
Int Arch Allergy Immunol ; 177(3): 238-244, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29975942

RESUMEN

BACKGROUND: Dental workers often experience unwanted allergic and nonallergic skin reactions resulting in different contact dermatoses (e.g., contact urticaria, irritant and allergic contact dermatitis) that are often attributed to rubber gloves. OBJECTIVE: To examine allergic and nonallergic contact dermatoses by different methods amongst dental professionals and dental students, more specifically, reactions to natural rubber latex (NRL), rubber additives, and other causative factors. METHODS: In this cross-sectional study we surveyed a total of 444 subjects (dentists, assistants, technicians, and students); 200 agreed to be tested to latex by the standard skin prick test (SPT) and prick-by-prick test, of whom 107 were patch tested to rubber additives (mercapto mix, thiuram mix, carba mix, and N-isopropyl-N-phenyl-4-phenylenediamine [IPPD]). RESULTS: Skin lesions appeared significantly more frequently with longer work experience (p = 0.002; V = 0.181), frequent glove changes (p < 0.001; V = 0.310), and hand washing (p < 0.001; V = 0.263), and in subjects with a history of allergies (atopic dermatitis, allergic rhinitis, allergic conjunctivitis, and others) (p < 0.001; V = 0.183). Positive SPTs to latex occurred in 14/200 subjects (7%), of whom 5/14 subjects (35.7%) were also positive in prick-by-prick tests. Patch tests were positive in 5/104 subjects (4.8%) (mercapto mix 1%, thiuram mix 1.9%, and carba mix 1.9%). CONCLUSION: Only a small number of our subjects were allergic to latex (7%) or rubber additives (4.8%). Thus, self-reported contact dermatoses (during NRL product use) in dental professionals and students are not commonly caused by allergies to latex and rubber additives, as is often assumed, but by other factors.


Asunto(s)
Dermatitis Alérgica por Contacto/epidemiología , Dermatitis Profesional/epidemiología , Guantes Protectores/efectos adversos , Hipersensibilidad al Látex/epidemiología , Látex/inmunología , Estudios Transversales , Odontólogos , Dermatitis Alérgica por Contacto/inmunología , Dermatitis Profesional/inmunología , Ditiocarba , Guanidinas/inmunología , Humanos , Látex/efectos adversos , Hipersensibilidad al Látex/inmunología , Pruebas del Parche , Fenilendiaminas/inmunología , Goma/química , Estudiantes , Compuestos de Sulfhidrilo/inmunología , Tiram/inmunología
14.
Curr Opin Allergy Clin Immunol ; 18(3): 168-176, 2018 06.
Artículo en Inglés | MEDLINE | ID: mdl-29629956

RESUMEN

PURPOSE OF REVIEW: Allergen immunotherapy (AIT) is a well recognized and extensively studied therapeutic intervention for some allergic diseases. Every year new systematic reviews and meta-analysis provide the most powerful source of evidence to orient decision making on prevention or efficacy of AIT. We here discuss systematic reviews and meta-analyses on AIT (published January 2017 to February 2018). RECENT FINDINGS: We identified 4 systematic reviews and 10 meta-analyses. Subcutaneous and sublingual AIT (SCIT/SLIT) significantly reduced the development of asthma in children-adolescents with moderate/severe allergic rhinitis, but evidence for a short-term, preventive effect for new allergic sensitizations was nonconclusive. SCIT/SLIT proved to significantly improve symptom and medication scores versus placebo or active comparators in respiratory allergy, with various effect sizes. Oral immunotherapy for IgE-mediated food allergy resulted in substantial benefit in desensitization rates. Data on venom AIT and latex AIT, although limited in quantity and quality, respectively, suggest a large and discrete beneficial effect. SUMMARY: Although current evidence extracted from meta-analyses and systematic reviews support AIT as an effective, relatively safe, and well tolerated alternative for some allergic diseases, heterogeneity and some methodological inconsistencies represent matters of concern as they may affect the validity and applicability of their results, especially in the context of individual, real-life settings.


Asunto(s)
Alérgenos/administración & dosificación , Asma/prevención & control , Desensibilización Inmunológica/métodos , Hipersensibilidad a los Alimentos/terapia , Hipersensibilidad al Látex/terapia , Rinitis Alérgica/terapia , Administración Oral , Administración Sublingual , Alérgenos/inmunología , Asma/inmunología , Hipersensibilidad a los Alimentos/inmunología , Humanos , Inyecciones Subcutáneas , Hipersensibilidad al Látex/inmunología , Metaanálisis como Asunto , Rinitis Alérgica/inmunología , Revisiones Sistemáticas como Asunto , Resultado del Tratamiento
15.
Mol Immunol ; 100: 21-27, 2018 08.
Artículo en Inglés | MEDLINE | ID: mdl-29650229

RESUMEN

We are now in the epoch of "molecular allergology" and numerous clinically relevant allergenic molecules are available improving the performance of in vitro allergen tests and allergen detection methods. This review is focusing on characterized occupational allergens and their implementation into the in vitro diagnosis for occupational allergy and in allergen detection methods. More than 400 occupational agents are identified and documented as being 'respiratory sensitizers', but currently only a limited number of them are characterized on the molecular level and available for routine diagnosis as native or recombinant allergens. One exception, however, is natural rubber latex (NRL) from Hevea brasiliensis still remaining an important occupational allergen source. Characterization of 15 NRL allergens led to the development of assays for the determination of allergen content of NRL materials and the implementation of component-resolved diagnosis (CRD) for specific IgE antibody measurement. Microarray or singleplex using recombinant or native allergens are reliable tools for NRL allergy diagnosis. In addition, NRL allergy is an excellent model for improving extract-based specific IgE measurement by amplification of NRL extract preparation with stable recombinant major allergen rHev b 5. Despite the many efforts to characterize the occupationally relevant wheat allergens for baker's asthma, the most frequently occurring forms of occupational asthma, the results are highly diverse. Wheat sensitization profiles of bakers showed great interindividual variability and no wheat allergen could be classified as the major allergen. For diagnosis of baker's asthma, a whole wheat extract is still the best option for specific IgE determination. But single wheat allergens might help to discriminate between wheat-induced food allergy, grass pollen allergy and baker's asthma. For workplace-related allergens like coffee, wood, soybean, seafood and moulds allergens are characterized and few of them are available, but their relevance for occupational sensitization routes should be verified in the further studies.


Asunto(s)
Alérgenos/inmunología , Asma Ocupacional/inmunología , Animales , Hipersensibilidad a los Alimentos/metabolismo , Humanos , Inmunoglobulina E/inmunología , Hipersensibilidad al Látex/inmunología , Polen/inmunología
16.
Int Arch Allergy Immunol ; 176(1): 1-7, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29590653

RESUMEN

BACKGROUND: It has been suggested that latex-specific IgE analysis may lead to false-positive results, especially in patients with pollen allergy. In the present study, the reasons underlying clinically irrelevant latex-specific IgE positivity were investigated. METHODS: Thirty patients with latex allergy (group 1), 89 patients sensitised to aeroallergens (group 2a), and 98 healthy individuals without allergy (group 2b) were enrolled. Participants from all 3 groups were subjected to skin prick tests with aeroallergens including latex, latex-specific IgE analysis (ImmunoCAP), and nasal provocation test with latex. All cases demonstrating positive latex-specific IgE also underwent specific IgE tests (ImmunoCAP) with latex profilin, birch pollen profilin, peach lipid transfer protein, and pineapple bromelain as cross-reactive carbohydrate determinants. RESULTS: Comparison of the atopic and healthy control groups showed that the rate of positive latex-specific IgE was significantly higher in group 2a. Latex profilin-, birch pollen profilin-, and bromelain-specific IgE were remarkably higher in group 2a. CONCLUSION: False positivity to latex-specific IgE in ImmunoCAP analysis may be observed in approximately 19% of patients with pollen allergy. Profilins and bromelain are the main contributors to clinically irrelevant positive latex-specific IgE.


Asunto(s)
Alérgenos/inmunología , Antígenos de Plantas/inmunología , Bromelaínas/inmunología , Proteínas Portadoras/inmunología , Hipersensibilidad al Látex/diagnóstico , Proteínas de Plantas/inmunología , Profilinas/inmunología , Rinitis Alérgica Estacional/diagnóstico , Adulto , Ananas/inmunología , Biomarcadores/sangre , Estudios de Casos y Controles , Reacciones Cruzadas , Reacciones Falso Positivas , Femenino , Humanos , Inmunoglobulina E/sangre , Hipersensibilidad al Látex/sangre , Hipersensibilidad al Látex/inmunología , Masculino , Persona de Mediana Edad , Prunus persica/inmunología , Rinitis Alérgica Estacional/sangre , Rinitis Alérgica Estacional/inmunología , Pruebas Cutáneas
18.
Artículo en Inglés | MEDLINE | ID: mdl-28760720

RESUMEN

Profilin is a protein that is present in all eukaryotic cells and is responsible for cross-reactivity between pollen, latex, and plant foods. It has been classically acknowledged as a minor or nearly irrelevant allergen, although recent data are changing this conception. The objective of this manuscript is to provide a comprehensive review of published data on the role of this ubiquitous allergen in pollen, latex, and plant food allergy. The patterns of recognition of this minor allergen follow a north-south gradient. Although present in all pollens and vegetables, profilin is significantly associated with allergy to grass pollen and to Cucurbitaceae fruits. Heb v 8, the latex profilin, is usually a marker of profilin allergy in plant food-allergic patients, although it has no clinical relevance in latex allergy. Sensitization to profilin jeopardizes the diagnosis of pollen allergy and selection of immunotherapy, and although component-resolved diagnosis can identify its impact, there are no tailored treatments available. In recent years, several new publications have shown how profilin should be taken into account and, under certain circumstances, considered a marker of severity, an allergen capable of inducing respiratory symptoms, and, in its natural purified form, a potential candidate for etiological treatment of food allergy. Current data on profilin strongly support the need for a shift in the previously accepted paradigm for this allergen. More research should be done to assess the real clinical impact of sensitization in specific populations and to develop therapeutic strategies.


Asunto(s)
Profilinas/inmunología , Alérgenos/inmunología , Hipersensibilidad a los Alimentos/inmunología , Humanos , Látex/inmunología , Hipersensibilidad al Látex/inmunología , Proteínas de Plantas/inmunología , Polen/inmunología , Rinitis Alérgica Estacional/inmunología
20.
Ann Allergy Asthma Immunol ; 118(5): 603-607, 2017 05.
Artículo en Inglés | MEDLINE | ID: mdl-28477790

RESUMEN

BACKGROUND: Atopic diseases have increased since the second half of the previous century. Atopic workers are at higher risk to be sensitized to latex, and the first years of exposure are supposed to be especially risky. OBJECTIVE: To assess atopic status and rate of latex sensitization in health care students starting their exposure to latex gloves. METHODS: We analyzed medical surveillance data from 1,628 health care students from 2010 to 2016. Students completed a questionnaire focused on their previous and current latex exposure and personal and family histories of allergic diseases; underwent skin prick testing with common allergens and latex extract (and/or total and latex-specific immunoglobulin E in serum); and underwent a medical examination. RESULTS: Skin prick test results for common inhalant allergens showed that 807 of 1,628 students (49.6%) had atopy. Atopy by skin prick testing was associated with male sex (odds ratio 1.49, 95% confidence interval 1.18-1.86), a personal history of oculorhinitis or asthma (odds ratio 10.22, 95% confidence interval 7.4-14.13), and atopic eczema (odds ratio 1.87, 95% confidence interval 1.05-3.36) at multivariate regression analysis. Eleven students (0.7% of total population) were found to be sensitized to latex and all had atopy. CONCLUSION: Despite the high prevalence of atopy in health care students of Trieste, the latex sensitization rate is very low and comparable to general population. This is reasonably due to the low exposure to latex gloves at the time of the evaluation and to low latex release from the gloves currently used in our hospital.


Asunto(s)
Alérgenos/inmunología , Instituciones de Salud , Hipersensibilidad Inmediata/tratamiento farmacológico , Hipersensibilidad Inmediata/inmunología , Hipersensibilidad al Látex/epidemiología , Hipersensibilidad al Látex/inmunología , Látex/efectos adversos , Estudiantes , Adolescente , Adulto , Estudios Transversales , Femenino , Personal de Salud , Humanos , Hipersensibilidad Inmediata/diagnóstico , Hipersensibilidad al Látex/diagnóstico , Masculino , Enfermedades Profesionales , Exposición Profesional , Oportunidad Relativa , Fenotipo , Vigilancia en Salud Pública , Factores de Riesgo , Pruebas Cutáneas , Encuestas y Cuestionarios , Adulto Joven
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