RESUMEN
PURPOSE: In the present study, we focused on the accessory middle colic artery and aimed to increase the safety and curative value of colorectal cancer surgery by investigating the artery course and branching patterns. METHODS: We included 143 cases (mean age, 70.4 ± 11.2 years; 86 males) that had undergone surgery for neoplastic large intestinal lesions at the First Department of Surgery at Yamagata University Hospital between August 2015 and July 2018. We constructed three-dimensional (3D) computed tomography (CT) angiograms and fused them with reconstructions of the large intestines. We investigated the prevalence of the accessory middle colic artery, the variability of its origin, and the prevalence and anatomy of the arteries accompanying the inferior mesenteric vein at the same level as the origin of the inferior mesenteric artery. RESULTS: Accessory middle colic artery was observed in 48.9% (70/143) cases. This arose from the superior mesenteric artery in 47, from the inferior mesenteric artery in 21, and from the celiac artery in two cases. In 78.2% (112/143) cases, an artery accompanying the inferior mesenteric vein was present at the same level as the origin of the inferior mesenteric artery; this artery was the left colic artery in 92, the accessory middle colic artery in 11, and it divided and became the left colic artery and the accessory middle colic artery in 10 cases. CONCLUSION: 3D CT angiograms are useful for preoperative evaluation. Accessory middle colic arteries exist and were observed in 14.9% of cases.
Asunto(s)
Variación Anatómica , Intestino Grueso/irrigación sanguínea , Anciano , Anciano de 80 o más Años , Arterias/anatomía & histología , Neoplasias Colorrectales/cirugía , Angiografía por Tomografía Computarizada , Femenino , Humanos , Imagenología Tridimensional , Intestino Grueso/cirugía , Masculino , Venas Mesentéricas/anatomía & histología , Persona de Mediana EdadRESUMEN
BACKGROUND: Leakage of the anastomosis after colorectal surgery is a severe complication, and one of the most important causes is poor vascular supply. However, a microvascular deficit is often not detectable during surgery under white light. Near-infrared indocyanine green (ICG)-enhanced fluorescence may be useful for assessing microvascular deficits and conceivably preventing anastomotic leakage. OBJECTIVES: This paper presents a preliminary retrospective case series on robotic colorectal surgery. The aim is to evaluate the feasibility, safety and role of near-infrared ICG-enhanced ?uorescence for the intraoperative assessment of peri-anastomotic tissue vascular perfusion. MATERIALS AND METHODS: From among more than 164 robotic colorectal cases performed, we retrospectively analyzed 28 that were all performed by the same surgeon (PCG) using near-infrared ICG-enhanced fluorescence technology: 16 left colectomies (57.1%), 8 rectal resections (28.6%), 3 right colectomies (10.8%) and 1 pancolectomy (3.6%). RESULTS: The rates of conversion, intraoperative complications, dye allergic reaction and mortality were all 0%. In two cases (7.1%)-1 left and 1 right colectomy-the level of the anastomosis was changed intraoperatively after ICG showed ischemic tissues. Despite the application of ICG, one anastomotic leak (after left colectomy for a chronic recurrent sigmoid diverticulitis with pericolic abscess) was observed. CONCLUSIONS: ICG technology may help to determine when to intraoperatively change the anastomotic level to a safer location. In our case series, ICG results led to a change in the level of the anastomosis in 7.1% of the cases. Despite the use of ICG, we observed one leak. This may have been related to vascularization-independent causes (e.g., infection in this case) or may reflect a need for better standardization of this ICG technology. In particular, we need a way to objectively assess the ICG signal and the related risk of leakage. More randomized, prospective, well-powered trials are needed to unveil the full potential of this innovative surgical technology.
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Anastomosis Quirúrgica/efectos adversos , Fuga Anastomótica/diagnóstico , Cirugía Colorrectal/efectos adversos , Colorantes , Verde de Indocianina , Rayos Infrarrojos , Fuga Anastomótica/etiología , Fuga Anastomótica/prevención & control , Cirugía Colorrectal/métodos , Fluorescencia , Intestino Grueso/irrigación sanguínea , Intestino Grueso/cirugía , Cuidados Intraoperatorios , Microvasos/diagnóstico por imagen , Estudios Retrospectivos , Procedimientos Quirúrgicos Robotizados/efectos adversosRESUMEN
Most intractable tissue-degenerative disorders share a common pathogenic condition, so-called proteinopathy. Amyloid-related disorders are the most common proteinopathies and are characterized by amyloid fibril deposits in the brain or other organs. Aging is generally associated with the development of these amyloid-related disorders, but we still do not fully understand how functional proteins become pathogenic amyloid deposits during the human aging process. We identified a novel amyloidogenic protein, named epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1), in massive venous amyloid deposits in specimens that we obtained from an autopsied patient who died of gastrointestinal bleeding. Our postmortem analyses of additional patients indicate that EFEMP1 amyloid deposits frequently developed in systemic venous walls of elderly people. EFEMP1 was highly expressed in veins, and aging enhanced venous EFEMP1 expression. In addition, biochemical analyses indicated that these venous amyloid deposits consisted of C-terminal regions of EFEMP1. In vitro studies showed that C-terminal regions formed amyloid fibrils, which inhibited venous tube formation and cell viability. EFEMP1 thus caused a novel age-related venous amyloid-related disorder frequently found in the elderly population. Understanding EFEMP1 amyloid formation provides new insights into amyloid-related disorders occurring during the aging process. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Asunto(s)
Amiloidosis/etiología , Proteínas de la Matriz Extracelular/metabolismo , Enfermedades Vasculares/etiología , Anciano de 80 o más Años , Biomarcadores/metabolismo , Proteínas de Unión al Calcio/metabolismo , Relación Dosis-Respuesta a Droga , Factor de Crecimiento Epidérmico/metabolismo , Proteínas de la Matriz Extracelular/fisiología , Femenino , Hemorragia Gastrointestinal/etiología , Células Endoteliales de la Vena Umbilical Humana , Humanos , Intestino Grueso/irrigación sanguínea , Venas/metabolismoRESUMEN
OBJECTIVE: Sensitivity of CT of the abdomen for detection of acute bowel ischemia (ABI) can be as low as 60%. In this study, we assessed the diagnostic performance of iodine-map and monoenergetic (40-keV) images in patients with suspected ABI. MATERIALS AND METHODS: This retrospective study included 60 consecutive patients with suspected ABI who underwent a standardized imaging protocol dual-source dual-energy CT (DECT) over a 26-month period. Clinical and operative outcomes were recorded as the reference standard. Two abdominal radiologists who were unaware of clinical and surgical information independently assessed conventional CT images for ABI. After a time interval designed to reduce recall, readers reassessed conventional images with supplementary iodine-map and 40-keV postprocessed images. Quantitative ROI analysis was also performed. RESULTS: ABI was observed in 11 patients. For reader A, sensitivity was 63.6% (95% CI, 30.8-89.1%) when interpreting conventional images alone. Sensitivity for detection of ABI increased to 81.8% (95% CI, 48.2-97.7%) and 100% (95% CI, 71.5-100%) when iodine-map and 40-keV images were assessed, respectively. For reader B, no change in sensitivity was seen with either technique, but the number of false-positives were reduced with supplementary iodine-map and 40-keV postprocessed images. Interobserver agreement was fair with conventional images (κ = 0.29) but improved to moderate (κ = 0.45) and substantial (κ = 0.63) with iodine-map and 40-keV images, respectively. Quantitative assessment found significant differences in iodine uptake (1.01 ± 0.55 mg/mL vs 3.04 ± 1.19 mg/mL) and mean attenuation (75.2 ± 38.4 HU vs 163.5 ± 48.9 HU) between nonischemic and ischemic segments, respectively. CONCLUSION: Iodine-map and 40-keV monoenergetic images increase conspicuity of ABI, resulting in improved diagnostic accuracy compared with review of conventional CT images alone.
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Medios de Contraste , Intestino Grueso/irrigación sanguínea , Intestino Delgado/irrigación sanguínea , Yohexol , Isquemia/diagnóstico por imagen , Tomografía Computarizada por Rayos X , Enfermedad Aguda , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Procesamiento de Imagen Asistido por Computador , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Sensibilidad y EspecificidadRESUMEN
BACKGROUND: Pneumatosis intestinalis (PI) is known as a sign of a life-threatening bowel ischemia. We aimed to evaluate the utility of intestinal fatty acid-binding protein (I-FABP) in the diagnosis of pathologic PI. METHODS: All consecutive patients who presented to our emergency department with PI were prospectively enrolled. The diagnostic performance of I-FABP for pathologic PI was compared with that of other traditional biomarkers and various parameters. RESULTS: Seventy patients with PI were enrolled. Pathologic PI was diagnosed in 27 patients (39%). The levels of most biomarkers were significantly higher in patients with pathologic PI than those with nonpathologic PI (P < .05). Receiver operator characteristic analysis revealed that the area under the curve (AUC) was highest for I-FABP (area under the curve = .82) in the diagnosis of pathologic PI. CONCLUSIONS: High I-FABP value, in combination with other parameters, might be clinically useful for pathologic PI.
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Proteínas de Unión a Ácidos Grasos/metabolismo , Intestino Grueso/irrigación sanguínea , Intestino Delgado/irrigación sanguínea , Isquemia/patología , Adulto , Anciano , Área Bajo la Curva , Biomarcadores/metabolismo , Estudios de Cohortes , Servicio de Urgencia en Hospital , Femenino , Humanos , Enfermedades Intestinales/mortalidad , Enfermedades Intestinales/patología , Enfermedades Intestinales/cirugía , Intestino Grueso/patología , Intestino Delgado/patología , Isquemia/mortalidad , Isquemia/cirugía , Modelos Logísticos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Valor Predictivo de las Pruebas , Pronóstico , Sistema de Registros , Estudios Retrospectivos , Medición de Riesgo , Tasa de Supervivencia , Resultado del TratamientoAsunto(s)
Malformaciones Arteriovenosas/diagnóstico por imagen , Malformaciones Arteriovenosas/patología , Várices/diagnóstico por imagen , Várices/patología , Adulto , Colon Sigmoide/irrigación sanguínea , Angiografía por Tomografía Computarizada , Humanos , Mucosa Intestinal/irrigación sanguínea , Intestino Grueso/irrigación sanguínea , Masculino , Mesenterio/irrigación sanguínea , Recto/irrigación sanguíneaRESUMEN
Constipation is a common symptom of patients presenting to the emergency department. If poorly managed, it can have significant consequences. A 50-year-old man was admitted to the emergency department following cardiac arrest. His medical history included a diagnosis of cerebral palsy and multiple admissions to hospital with constipation. Clinical examination revealed a grossly distended and tense abdomen. Circulatory supply to the lower limbs was acutely impaired. CT scan revealed massive dilation of the large bowel due to faecal loading with compromised circulation to the lower limbs. Despite aggressive attempts at resuscitation, the patient continued to deteriorate and passed away. On reflection, a diagnosis of abdominal compartment syndrome secondary to severe constipation was made. This case highlights the vigilance required when managing what is often felt to be a trivial complaint and the considerable consequences when there is a failure to do so.
Asunto(s)
Abdomen/patología , Estreñimiento/complicaciones , Paro Cardíaco/etiología , Intestino Grueso/patología , Hipertensión Intraabdominal/diagnóstico , Abdomen/irrigación sanguínea , Resultado Fatal , Humanos , Intestino Grueso/irrigación sanguínea , Hipertensión Intraabdominal/etiología , Masculino , Persona de Mediana Edad , Presión , Flujo Sanguíneo Regional , Resucitación , Vena Cava InferiorRESUMEN
Hernias are routine general surgical problems that may present in any age group, regardless of the patient's socioeconomic status. We present a rare case of a complicated ventral hernia leading to short bowel. This is an unusual case and is very rarely reported in the literature. This current case report describes a 54-year-old gentleman who presented to the hospital with a giant strangulated ventral hernia causing massive bowel ischemia and resulting in a short bowel. The literature on large abdominal wall hernias leading to short bowel is reviewed, and a discussion on short bowel syndrome is also presented.
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Hernia Ventral/complicaciones , Hernia Ventral/cirugía , Intestino Grueso/irrigación sanguínea , Intestino Delgado/irrigación sanguínea , Isquemia/etiología , Isquemia/cirugía , Síndrome del Intestino Corto/etiología , Anastomosis Quirúrgica , Gangrena , Hernia Ventral/patología , Humanos , Intestino Grueso/patología , Intestino Grueso/cirugía , Intestino Delgado/patología , Intestino Delgado/cirugía , Isquemia/patología , Masculino , Persona de Mediana Edad , Resultado del TratamientoAsunto(s)
Isquemia/diagnóstico por imagen , Isquemia/patología , Isquemia Mesentérica/diagnóstico por imagen , Isquemia Mesentérica/patología , Estómago/irrigación sanguínea , Estómago/diagnóstico por imagen , Reanimación Cardiopulmonar/efectos adversos , Progresión de la Enfermedad , Resultado Fatal , Femenino , Humanos , Intestino Grueso/irrigación sanguínea , Intestino Grueso/patología , Intestino Delgado/irrigación sanguínea , Intestino Delgado/patología , Isquemia/cirugía , Isquemia Mesentérica/cirugía , Persona de Mediana Edad , Infarto del Miocardio/terapia , Necrosis , Estómago/patología , Rotura Gástrica/diagnóstico por imagen , Rotura Gástrica/patología , Rotura Gástrica/cirugía , Tomografía Computarizada por Rayos X , Privación de TratamientoRESUMEN
Experimental comparative morphological investigation of hemomicrocirculation bed (HMCB) of the small and large bowel wall was performed in dynamics of an acute serous peritonitis. Spreaded aseptic peritonitis was simulated using injection of 5 ml of gamma-caraginen (Sigma, USA) in 1 ml of isotonic solution of sodium chloride. On the early stage of peritonitis (in 12 h from beginning of the experiment) in mucosa of small bowel nonsignificant venuls dilatation and the capillary lumen reduction were observed. In 1 day (reactive stage of peritonitis) in mucosa the quantity of capillars have had reduced significantly, comparing with such observed previously. On the 2-nd day (toxic stage of peritonitis) some capillary dilatation in intestinal villi and crypts coexistant with the blood rheology disorders in a form of stasis, change in permeability of the vessels walls, predominantly of the venous, was noted. On the 3-d day (late stage) the arteriol's spasm have had reduced, capillary paralytic dilatation was revealed. The staged course of experimental peritonitis with the HMCB changes, characteristic for every stage, was confirmed, basing on analysis of the investigation result.
Asunto(s)
Intestino Grueso/irrigación sanguínea , Intestino Delgado/irrigación sanguínea , Microcirculación/fisiología , Microvasos/fisiopatología , Peritonitis/fisiopatología , Enfermedad Aguda , Animales , Modelos Animales de Enfermedad , Mucosa Intestinal/irrigación sanguínea , Mucosa Intestinal/fisiopatología , Intestino Grueso/fisiopatología , Intestino Delgado/fisiopatología , Microvasos/patología , Peritonitis/patología , Ratas , Ratas Wistar , Factores de Tiempo , Vasodilatación/fisiologíaRESUMEN
During anatomical dissection of the abdominal cavity of an adult male corpse, a changing pattern of vascular supply of pancreas and of the transverse colon was observed. The inferior pancreatic artery and the middle colic artery arose from a common trunk of the superior mesenteric artery. Moreover, the lienal artery did not direct a dorsal branch to the neck of the pancreas, but rather sent two greater pancreatic arteries (proximal and distal) to the body of the gland and one caudal artery to the terminal portion of the pancreas. We also observed a large communicating branch between the proximal portion of the middle colic artery and the ascending branch of the left colic artery, previously described as artery of Moskowitz. Based on these data, clinical and surgical implications of these variations were highlighted.
Durante la disección anatómica de la cavidad abdominal del cadáver de un hombre adulto, se observó un patrón cambiante en el suministro vascular del páncreas y del colon transverso. La arteria pancreática inferior y la arteria cólica media surgían de un tronco común desde la arteria mesentérica superior. Además, la arteria esplénica no daba origen a la rama dorsal hasta el cuello del páncreas, más bien contaba con dos arterias pancreáticas mayores (una proximal y una distal) hasta la glándula, y una arteria caudal. También se observó una conexión entre la parte proximal de la arteria cólica media y el ramo ascendente de la arteria cólica izquierda lo que fue previamente definido como la arteria de «Moskowitz¼. Las implicaciones quirúrgicas de estas variaciones fueron destacadas en base a estos datos.
Asunto(s)
Humanos , Masculino , Adulto , Páncreas/irrigación sanguínea , Intestino Grueso/irrigación sanguínea , Cadáver , Colon Transverso/irrigación sanguínea , Variación AnatómicaRESUMEN
The blood supply in the large intestine of seven specimens of the lesser anteater, Tamandua tetradactyla, studied. The method included preparation of the macroscopic collection report, perfusion of the arterial network with water, injection of colored latex, fixation in formaldehyde, and preservation in ethanol. For our description and analyses, we performed dissections under mesoscopic light and made photo documentation of our observations. The large intestine of T. tetradactyla is irrigated by the caudal mesenteric artery (rectum, left colic fold, descending colon and transverse colon) and cranial mesenteric artery (right colic fold, cecal pouch). We observed that the large intestine in these animals is implied in the abdominal wall without becoming affixed to the wall, or developing adhesions on individual segments. The caudal mesenteric artery feeds the straight collateral branches (primary, secondary, and tertiary) and a few juxtacolic arched branches (first and second order). The straight branches emerge from the arched branches, bifurcate, and embrace the intestinal loop to irrigate it. The presence of anastomoses between the CaMA and the CrMA apparently ensures a relatively stable flow in the event of failure of either. This is very important, as the peritoneum in this species is completely dependent on blood from these two arteries. The model of vascularization and fixation of the large intestine into the abdominal wall of T. tetradactyla is different from that in other vertebrates.
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Arterias/anatomía & histología , Intestino Grueso/irrigación sanguínea , Xenarthra/anatomía & histología , Animales , Femenino , Intestino Grueso/anatomía & histología , MasculinoRESUMEN
Symptomatic chronic mesenteric ischemia results from intestinal hypoperfusion and is classically thought to result from involvement of two or more mesenteric arteries. The celiac artery and superior mesenteric artery are most frequently implicated in this disease process, and their involvement usually results in symptoms of small intestinal ischemia. Symptomatic chronic mesenteric ischemia resulting predominantly from inferior mesenteric artery involvement has largely been overlooked but does gives rise to its own, unique clinical presentation with symptoms resulting from large intestinal ischemia. We present four patients with atherosclerotic inferior mesenteric artery stenosis with symptomatic chronic mesenteric ischemia that have unique clinical presentations consistent with large intestinal ischemia that resolved following percutaneous endovascular treatment of the inferior mesenteric artery stenosis. These cases represent a novel approach to the diagnosis and management of this disease process and may warrant a further subclassification of chronic mesenteric ischemia into chronic small intestinal ischemia and chronic large intestinal ischemia.
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Aterosclerosis/complicaciones , Intestino Grueso/irrigación sanguínea , Isquemia/etiología , Oclusión Vascular Mesentérica/etiología , Circulación Esplácnica , Enfermedades Vasculares/etiología , Anciano , Anciano de 80 o más Años , Angioplastia de Balón/instrumentación , Aterosclerosis/diagnóstico por imagen , Aterosclerosis/fisiopatología , Aterosclerosis/terapia , Constricción Patológica , Femenino , Humanos , Isquemia/diagnóstico por imagen , Isquemia/fisiopatología , Isquemia/terapia , Arteria Mesentérica Inferior/diagnóstico por imagen , Arteria Mesentérica Inferior/fisiopatología , Isquemia Mesentérica , Oclusión Vascular Mesentérica/diagnóstico por imagen , Oclusión Vascular Mesentérica/fisiopatología , Oclusión Vascular Mesentérica/terapia , Persona de Mediana Edad , Radiografía , Flujo Sanguíneo Regional , Factores de Riesgo , Stents , Resultado del Tratamiento , Enfermedades Vasculares/diagnóstico por imagen , Enfermedades Vasculares/fisiopatología , Enfermedades Vasculares/terapiaRESUMEN
OBJECTIVES: The potential to predict, and therefore avoid, anastomotic failure has eluded generations of colon and rectal surgeons to date. A reliable, reproducible method of assessing bowel blood flow therefore would be of enormous potential clinical relevance. To our knowledge, intraoperative contrast-enhanced sonography of the bowel has not been performed previously. We present our study assessing the feasibility of using contrast-enhanced sonography to study bowel perfusion intraoperatively. METHODS: We studied 8 patients (4 male and 4 female) with an age range of 52 to 81 years who underwent colorectal surgery (right hemicolectomies, n = 3; Hartmann procedure, n = 1; anterior resections, n = 2; and bowel resections with ileocolic anastomoses, n = 2). A 5-mL bolus of a sulfur hexafluoride contrast agent solution was injected before and after vascular ligation with simultaneous noncompression ultrasound scanning directly over the large bowel. The patients were followed clinically to assess for leaks. Contrast-enhanced sonographic time-intensity curves were generated for the time to peak and maximum amplitude. RESULTS: Moderate interobserver agreement was shown for the time to peak (κ = 0.50) and maximum amplitude (κ = 0.42), and moderate intraobserver agreement was shown for the time to peak (κ= 0.53) and maximum amplitude (κ= 0.53). No significant differences were shown between the time to peak (P = .28) and maximum amplitude (P = .49) for the preligation and postligation scans. CONCLUSIONS: To our knowledge, intraoperative contrast-enhanced sonography of the bowel has not been performed previously. We have shown the technique to be feasible with good intraobserver and interobserver agreement. Further work is ongoing to optimize the technique and assess its use in predicting anastomotic breakdown.
Asunto(s)
Medios de Contraste , Aumento de la Imagen/métodos , Intestino Grueso/irrigación sanguínea , Intestino Grueso/diagnóstico por imagen , Complicaciones Intraoperatorias/diagnóstico por imagen , Monitoreo Intraoperatorio/métodos , Anciano , Anciano de 80 o más Años , Anastomosis Quirúrgica , Colon/irrigación sanguínea , Colon/diagnóstico por imagen , Colon/cirugía , Estudios de Factibilidad , Femenino , Humanos , Intestino Grueso/cirugía , Masculino , Persona de Mediana Edad , Variaciones Dependientes del Observador , Fosfolípidos , Estudios Prospectivos , Recto/irrigación sanguínea , Recto/diagnóstico por imagen , Recto/cirugía , Reproducibilidad de los Resultados , Hexafluoruro de Azufre , UltrasonografíaRESUMEN
Basing on statistical analysis of 17 prognostic risk factors for occurrence of the sutures insufficiency in a large bowel anastomoses (SILBA) in 237 patients there was established a trustworthy correlational connection for 7 factors. The results of a regression analysis have had confirmed the influence of the anastomosis formation method on the complication rate, demonstrated by an active inflammation in the anastomosis formation area, technical faults while the wall mobilization and rude formation of sutures, intramural disorder of the blood circulation, the blood circulation general disorders, local infectioning and hypoproteinemia. Application of analysis of the tables of conjunction and comparison in accordance with the numeral and interval predictors, using nonparametric analysis (the method of a binary logistic regression), have permitted to delineate the most prognostically significant risk factors of the SILBA occurrence.
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Fuga Anastomótica/etiología , Colostomía/métodos , Intestino Grueso/cirugía , Procedimientos de Cirugía Plástica/efectos adversos , Técnicas de Sutura/efectos adversos , Suturas , Anastomosis Quirúrgica , Humanos , Intestino Grueso/irrigación sanguínea , Pronóstico , Flujo Sanguíneo Regional , Análisis de Regresión , Factores de RiesgoRESUMEN
BACKGROUND: Intestinal damage after ischemia followed by revascularization, referred to as "ischemia-reperfusion (I/R) injury," is a devastating complication that can occur after acute superior mesenteric obstruction, or after both elective and emergent abdominal aortic surgery. Once an entire layer of intestine is involved in severe ischemia, the mortality rate reaches 90%; no effective medical treatment has been reported to date. Here, we demonstrate that a somatostatin analogue, octreotide, but not a free-radical scavenger, MCI-186, prevented death due to surgically induced intestinal I/R injury in rats. METHODS: Superior mesenteric artery (SMA) of Male Sprague-Dawley rats, that received MCI-186 or octreotide, was surgically clamped, and then the clips were removed and SMA blood flow restored. Survival was assessed, and blood and small intestine were subjected to cell count, enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunohistochemistry. RESULTS: Of interest, pretreatment with octreotide, but not with MCI-186, just before induced intestinal ischemia prompted the early expression of heme oxygenase-1 (HO-1) protein-associated accumulation of CD68-positive cells, a possible cellular source of HO-1. Inversely, the administration of tin protoporphyrin IX (SnPPN), a specific inhibitor of HO-1, completely abolished the therapeutic effects of octreotide, indicating that the favorable effects of octreotide against intestinal I/R injury is predominantly dependent on the early induction of HO-1. CONCLUSIONS: These results suggest that a somatostatin analogue may be useful in leading to an improvement of the prognosis of patients with intestinal I/R injury in the clinical setting.
Asunto(s)
Hemo-Oxigenasa 1/metabolismo , Intestino Grueso/irrigación sanguínea , Intestino Grueso/metabolismo , Octreótido/uso terapéutico , Daño por Reperfusión/prevención & control , Somatostatina/análogos & derivados , Animales , Antipirina/análogos & derivados , Antipirina/uso terapéutico , Edaravona , Inhibidores Enzimáticos/farmacología , Depuradores de Radicales Libres/uso terapéutico , Hemo-Oxigenasa 1/antagonistas & inhibidores , Masculino , Metaloporfirinas/farmacología , Modelos Animales , Protoporfirinas/farmacología , Ratas , Ratas Sprague-Dawley , Flujo Sanguíneo Regional/fisiologíaRESUMEN
A variety of factors are critical for the success of bowel anastomoses. The most crucial patient factor is adequate vascularity of the bowel ends which are to be anastomosed. Currently, intraoperative features such as healthy looking bleeding edges of the bowel are considered to be signs of adequate vascular supply. However, once the anastomosis is performed, external appearances may not be reliable. In order to improve evaluation of the bowel as well as the anastomosis, our group has adopted the routine use of post-anastomosis intraoperative colonoscopy. Intraoperative colonoscopy provides vital information regarding the integrity of the anastomosis (leak testing) and also visualizes the mucosa of the bowel. Narrow Band Imaging (NBI) is a novel real-time imaging technique which is an integral component of many modern colonoscopes. We hypothesize that NBI assessment of vascularity at the time of intestinal anastomosis can improve safety and reduce the risks of anastomotic complications following surgery.
Asunto(s)
Anastomosis Quirúrgica/métodos , Colonoscopía/métodos , Intestino Grueso/irrigación sanguínea , Intestino Grueso/cirugía , Monitoreo Intraoperatorio/métodos , HumanosRESUMEN
BACKGROUND: The continuous monitoring of splanchnic organ oxygen saturation could make the early detection of inadequate tissue oxygenation feasible, reducing the risk of hypoperfusion, severe ischemia, multiple organ failure, and, ultimately, death. Current methods for assessing splanchnic perfusion have not been widely accepted for use in the clinical care environment. In an attempt to overcome the limitations of the current techniques, a new fiberoptic photoplethysmographic (PPG)/pulse oximetry sensor was developed as a means of assessing splanchnic organ perfusion during surgery in humans. METHODS: A new fiberoptic splanchnic pulse oximeter and an optically identical fiberoptic finger pulse oximeter have been developed. Simultaneous PPG signals and preliminary estimates of arterial oxygen saturation from the bowel (small and large) and finger were obtained in 17 patients (3 men and 14 women) undergoing open laparotomy. RESULTS: Good quality PPG signals were obtained from the small and large bowel and from the finger in all patients (lower 95% confidence limit for the proportion was 0.64). Comparisons of blood oxygen saturation values acquired when using the splanchnic and the finger fiberoptic sensors and a commercial finger pulse oximeter indicated that there was no statistically significant difference between them (all P>0.454). A Bland and Altman plot of the difference between blood oxygen saturation values from the bowel fiberoptic pulse oximeter and the fiberoptic finger pulse oximeter against their mean showed that the limits of agreement between the 2 pulse oximeters were -3.8% and 4.2% for small bowel measurements, and -3.4% and 4.3% for large bowel measurements. The 95% prediction interval for the difference between the 2 devices was between -4.2% and 4.7%. CONCLUSION: This study demonstrated that good quality PPG signals can be obtained from the bowel using a new fiberoptic sensor. Further evaluation is required to determine whether fiberoptic pulse oximetry of the bowel may provide a suitable method for monitoring splanchnic perfusion.