RESUMEN
BACKGROUND: The intrusion of maxillary anterior teeth is often required and there are various intrusion modes with mini-implants in clear aligner treatment. The objective of this study was to evaluate the effectiveness of maxillary anterior teeth intrusion with different intrusion modes, aiming to provide references for precise and safe intrusion movements in clinical practice. METHODS: Cone-beam computed tomography and intraoral optical scanning data of a patient were collected. Finite element models of the maxilla, maxillary dentition, periodontal ligaments (PDLs), clear aligner (CA), attachments, and mini-implants were established. Different intrusion modes of the maxillary anterior teeth were simulated by changing the mini-implant site (between central incisors, between central and lateral incisor, between lateral incisor and canine), loading site (between central incisors, on central incisor, between central and lateral incisor, between lateral incisor and canine), and loading mode (labial loading and labiolingual loading). Ten conditions were generated and intrusive forces of 100 g were applied totally. Then displacement tendency of the maxillary anterior teeth and CA, and stress of the PDLs were analyzed. RESULTS: For the central incisor under condition L14 and for the canine under conditions L11, L13, L23, and L33, the intrusion amount was negative. Under other conditions, the intrusion amount was positive. The labiolingual angulation of maxillary anterior teeth exhibited positive changes under all conditions, with greater changes under linguoincisal loading. The mesiodistal angulation of canine exhibited positive changes under labial loading, while negative changes under linguoincisal loading except for condition L14. CONCLUSIONS: The intrusion amount, labiolingual and mesiodistal angulations of the maxillary anterior teeth were affected by the mini-implant site, loading site, and loading mode. Labial and linguoincisal loading may have opposite effects on the intrusion amount of maxillary anterior teeth and the mesiodistal angulation of canine. The labiolingual angulation of the maxillary incisors would increase under all intrusion modes, with greater increases under linguoincisal loading.
Asunto(s)
Tomografía Computarizada de Haz Cónico , Implantes Dentales , Análisis de Elementos Finitos , Incisivo , Maxilar , Métodos de Anclaje en Ortodoncia , Ligamento Periodontal , Técnicas de Movimiento Dental , Humanos , Técnicas de Movimiento Dental/métodos , Técnicas de Movimiento Dental/instrumentación , Métodos de Anclaje en Ortodoncia/instrumentación , Métodos de Anclaje en Ortodoncia/métodos , Ligamento Periodontal/diagnóstico por imagen , Imagenología Tridimensional/métodos , Diente Canino/diagnóstico por imagen , Diseño de Aparato Ortodóncico , Análisis del Estrés Dental , Fenómenos Biomecánicos , Aparatos Ortodóncicos RemoviblesRESUMEN
OBJECTIVE: To analyse the anterior teeth effects of clear aligners on five different patterns of mandibular molar movement and to define the most effective configuration to be implemented with clear aligners through finite element analysis. METHODS: A three-dimensional mandibular model with a deep overbite in the mandible was constructed using cone beam computerized tomography (CBCT) data. The model included the mandibular dentition, mandibular periodontal ligaments, attachments, and aligners. Five models were created: (1) configuration A: second molar distalization (0.25 mm); (2) configuration B: second molar distalization (0.25 mm), first molar extrusion (0.15 mm); (3) configuration C: second molar distalization (0.25 mmm), first and second premolar extrusion(0.15 mm); (4) configuration D: second molar distalization (0.25 mm), first molar and first/second premolar extrusion(0.15 mm); and (5) configuration E: second molar distalization (0.25 mm), first molar and first/second premolar extrusion (0.15 mm), first molar and first/second premolar expansion (0.15 mm). RESULTS: In all configurations, the anterior teeth exhibited labial tipping and the mandibular central incisor of configuration E showed the highest labial tipping. Configuration E demonstrated a relatively minor impact on mandibular molars distalization compared with configuration A. Configuration A showed the highest distal displacement value, and configuration E produced the lowest displacement value. Configuration E caused the highest periodontal ligament (PDL) pressure of the central and lateral incisors. The differences in the canines between configurations C and D,were not significant, and the stress distribution differed among the five groups. CONCLUSIONS: All patterns utilizing clear aligners facilitated mandibular molar distalization. Extruding the premolars and second molar distalization at the same time had little impact on second molar distalization; When expansion and extrusion were simultaneously performed during the distalization of mandibular molars, our prime consideration was the alveolar bone on the labial side of the anterior teeth to prevent the occurrence of gingival recession, dehiscence, and fenestration. Due to the lack of consideration for periodontal tissues in this study, clinical protocols should be designed based on the periodontal status of the mandibular anterior teeth.
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Diente Molar , Aparatos Ortodóncicos Removibles , Humanos , Análisis de Elementos Finitos , Diente Molar/diagnóstico por imagen , Incisivo/diagnóstico por imagen , Ligamento Periodontal/diagnóstico por imagen , Técnicas de Movimiento DentalRESUMEN
Various staging scales have been proposed for the assessment of the visibility of the periodontal ligament space of mandibular third molars on dental panoramic radiographs (PANs) for forensic age assessment in living individuals. However, up to now, there has been no systematic comparison between these staging scales available. We directly compared the 2010 staging scale proposed by Olze et al. with the 2017 staging by Lucas et al. and the 2020 staging by Guo et al. in a German study population. We evaluated 233 PANs from 115 females and 118 males aged 20.0 to 40.9 years using three independent examiners, with one examiner conducting two assessments. We examined the correlation between age and stage, as well as the inter- and intra-rater reliabilities. While the point estimates for the correlation coefficient and the reliability measures were lowest for the Guo scale and highest for the Olze scale, confidence intervals showed a large overlap, particularly for the scales of Olze et al. and Lucas et al. The correlation coefficients between stage and age were consistently lower in females than in males across all methods. In summary, we showed that the staging scales of Olze et al. and Lucas et al. were very similar. The Olze method showed higher point estimates across all analyses, and because there are more reference data available for this method, we argue that it should be preferred as the method of choice for further studies in the field. However, Guo method could be considered for instances, in which the inter-radicular periodontal ligament is not evaluable.
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Determinación de la Edad por los Dientes , Tercer Molar , Ligamento Periodontal , Radiografía Panorámica , Humanos , Tercer Molar/diagnóstico por imagen , Tercer Molar/crecimiento & desarrollo , Masculino , Femenino , Determinación de la Edad por los Dientes/métodos , Ligamento Periodontal/diagnóstico por imagen , Adulto , Adulto Joven , Reproducibilidad de los Resultados , Mandíbula/diagnóstico por imagen , Mandíbula/anatomía & histologíaRESUMEN
OBJECTIVES: To determine the effect of orthodontic pressure on periodontal ligament (PDL) compression in rats and assess correlation between PDL compression and orthodontically induced root resorption (OIRR). MATERIALS AND METHODS: Eight female Wistar rats aged 10 weeks underwent surgery to place 2 mini-screws at the center of the palatal plate. 25 cN coil springs connecting the maxillary first molars and mini-screws were applied bilaterally to generate mesial force. Maxillary first molars were assigned to undergo either bodily or tipping movements. Micro-computed tomography (µCT) scans were taken on days 0, 3, 7, and 14, and histological sections were taken on day 14. OIRR was measured from histological sections, and the corresponding PDL compression ratio was quantified using µCT images. RESULTS: The PDL was compressed by approximately 76% in tipping movement and 55% in bodily movement after 3 days, and by approximately 47% in bodily and tipping movements after 7 days of orthodontic force application. The extent of OIRR in tipping movement was significantly greater than that in bodily movement. A strong positive correlation between OIRR and PDL compression ratio was observed on day 3; however, no correlation was observed on day 7. CONCLUSIONS: A strong correlation between PDL compression ratio and OIRR was observed at an early stage after the application of orthodontic force regardless of the tooth movement type (bodily or tipping), implying the importance of early stage PDL compression in the induction of OIRR.
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Resorción Radicular , Ratas , Femenino , Animales , Resorción Radicular/diagnóstico por imagen , Resorción Radicular/etiología , Ratas Wistar , Ligamento Periodontal/diagnóstico por imagen , Microtomografía por Rayos X/métodos , Técnicas de Movimiento Dental/efectos adversos , Técnicas de Movimiento Dental/métodosRESUMEN
The structural process of bone and periodontal ligament (PDL) remodeling during long-term orthodontic tooth movement (OTM) has not been satisfactorily described yet. Although the mechanism of bone changes in the directly affected alveolar bone has been deeply investigated, detailed knowledge about specific mechanism of PDL remodeling and its interaction with alveolar bone during OTM is missing. This work aims to provide an accurate and user-independent analysis of the alveolar bone and PDL remodeling following a prolonged OTM treatment in mice. Orthodontic forces were applied using a Ni-Ti coil-spring in a split-mouth mice model. After 5 weeks both sides of maxillae were scanned by high-resolution micro-CT. Following a precise tooth movement estimation, an extensive 3D analysis of the alveolar bone adjacent to the first molar were performed to estimate the morphological and compositional parameters. Additionally, changes of PDL were characterized by using a novel 3D model approach. Bone loss and thinning, higher connectivity as well as lower bone mineral density were found in both studied regions. Also, a non-uniformly widened PDL with increased thickness was observed. The extended and novel methodology in this study provides a comprehensive insight about the alveolar bone and PDL remodeling process after a long-duration OTM.
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Ligamento Periodontal , Técnicas de Movimiento Dental , Ratones , Animales , Técnicas de Movimiento Dental/métodos , Ligamento Periodontal/diagnóstico por imagen , Remodelación ÓseaRESUMEN
BACKGROUND: Having a reliable and feasible method to estimate whether an individual has reached 16 years of age would greatly benefit forensic analysis. The study of age using dental information has matured recently. In addition, machine learning (ML) is gradually being applied for dental age estimation. AIM: The purpose of this study was to evaluate the development of the third molar using the Demirjian method (Demirjian3M), measure the development index of the third molar (I3M) using the method by Cameriere, and assess the periodontal ligament development of the second molar (PL2M). This study aimed to predict whether Chinese adolescents have reached the age of criminal responsibility (16 years) by combining the above measurements with ML techniques. SUBJECTS & METHODS: A total of 665 Chinese adolescents aged between 12 and 20 years were recruited for this study. The development of the second and third molars was evaluated by taking orthopantomographs. ML algorithms, including random forests (RF), decision trees (DT), support vector machines (SVM), K-nearest neighbours (KNN), Bernoulli Naive Bayes (BNB), and logistic regression (LR), were used for training and testing to determine the dental age. This is the first study to combine ML with an evaluation of periodontal ligament and tooth development to predict whether individuals are over 16 years of age. RESULTS AND CONCLUSIONS: The study showed that SVM had the highest Bayesian posterior probability at 0.917 and a Youden index of 0.752. This finding provides an important reference for forensic identification, and the combination of traditional methods and ML is expected to improve the accuracy of age determination for this population, which is of substantial significance for criminal litigation.
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Tercer Molar , Ligamento Periodontal , Adolescente , Humanos , Niño , Adulto Joven , Adulto , Tercer Molar/diagnóstico por imagen , Teorema de Bayes , Ligamento Periodontal/diagnóstico por imagen , Diente Molar/diagnóstico por imagen , Aprendizaje AutomáticoRESUMEN
OBJECTIVES: The purpose of this study was to evaluate the association between clinical manifestations of occlusal trauma of the teeth and maximum signal intensity of periodontal ligament space on MRI. METHODS: 20 subjects (males: 9, females: 11, mean age: 35.9 ± 14.0 years, range: 22-65 years) participated in this study. Subjective symptoms of bruxism, tooth mobility, fremitus, occlusal contact area, occlusal force, widening of the periodontal ligament space, and thickening of the lamina dura were defined as clinical manifestations of occlusal trauma. The total number of clinical manifestations was used to evaluate the degree of clinical occlusal trauma, with a score of 7 indicating the highest degree of occlusal trauma. The maximum signal intensity in the periodontal ligament space was evaluated by a specific T2 weighted MRI sequence: IDEAL image. RESULTS: Spearman's rank correlation between the total clinical occlusal trauma score and maximum signal intensity in the periodontal ligament space was 0.529 for all teeth, 0.517 for anterior teeth, and 0.396 for molar teeth (p < 0.001 for all). CONCLUSIONS: A significant correlation between the degree of occlusal trauma and the signal intensity of the periodontal ligament space suggests a new potential MRI-based method for objectively determining occlusal trauma.
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Oclusión Dental Traumática , Diente , Masculino , Femenino , Humanos , Adulto Joven , Adulto , Persona de Mediana Edad , Ligamento Periodontal/diagnóstico por imagen , Ligamento Periodontal/patología , Oclusión Dental Traumática/complicaciones , Oclusión Dental Traumática/diagnóstico por imagen , Fuerza de la Mordida , Imagen por Resonancia MagnéticaRESUMEN
Obstructive sleep apnea syndrome (OSAS) is recurrent apnoea caused by upper airway obstruction during sleep. In severe cases, OSAS might lead to sudden death. Currently, the mandibular advancement device (MAD) is the preferred product for the treatment of mild to moderate OSAS because of its compliance, portability and low cost. However, many clinical studies have suggested that long-term use of MAD might cause occlusal changes, periodontitis, muscle soreness, and joint damage. In view of the difficulties in the measurement of relevant mechanical factors in vivo, the present work aimed to quantitatively analyze biomechanical mechanisms that might lead to these side effects through computer numerical simulations and a nonhomogeneous alveolar bone model was established to approximate the actual bony features of the jaw in the simulations model. First, a 3D digital model of the teeth, periodontal ligament(PDL), and alveolar bone was created on the basis of computed tomography images and assembled with a 3D model of the MAD. A nonhomogeneous alveolar bone model was created based on computed tomographic images, and the stresses acting on the PDL were computed using the finite element method. The results showed that the nonhomogeneous model could more realistically reflect the mechanical properties of the alveolar bone and obtain the true stresses compared with the homogeneous model, which underestimated the adverse effects of PDL therapy. The numerical simulations in this paper can help doctors make more accurate judgements about MAD treatment from an oral health protection perspective.
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Avance Mandibular , Apnea Obstructiva del Sueño , Humanos , Ligamento Periodontal/diagnóstico por imagen , Ferulas Oclusales , Apnea Obstructiva del Sueño/terapia , Cabeza , Resultado del TratamientoRESUMEN
With a limited alveolar bone position, there is a high risk that mini-screws (MS) implants could cause damage to the adjacent teeth. To reduce this damage, the position and tilt angle of the MS must be optimized. The aim of this study was to assess the effect of MS implantation angle on the stress exerted on adjacent periodontal membrane and roots. A three-dimensional finite element model containing dentition, periodontal ligament, jaw and MS were established based on the CBCT images and MS scanning data. The MS was first inserted perpendicular to the surface of the bone at specific locations and then tilted at an angle of 10° and 20° to the mesial and distal teeth, respectively. The stress distribution in the periodontal tissue of the adjacent teeth was analyzed after MS implantation at different angles.The stress on the adjacent tooth root and periodontal ligament was most uniformly distributed when the MS was inserted vertically. It changed 9.4-97.7% when the axis of MS was tilted at 10-degree and 20-degree angles from the point of vertical insertion. The stresses experienced by the periodontal ligament and the root are similar. When the horizontal angle of the MS insertion was changed, the MS was closer to the adjacent tooth, resulting in greater stress near the PDL and root. It was recommended to insert the MS vertically into the alveolar bone surface to avoid root damage due to excessive stress.
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Tornillos Óseos , Ligamento Periodontal , Análisis del Estrés Dental , Análisis de Elementos Finitos , Ligamento Periodontal/diagnóstico por imagen , Estrés MecánicoRESUMEN
OBJECTIVES: To determine the expansion rebound deformation (ERD) of clear aligners (CAs) and its biomechanical influence. MATERIALS AND METHODS: A four-premolar extraction treatment plan was carried out for a patient with 2 CA companies. Thirty-six digitally scanned clear aligners with the corresponding 36 virtually constructed "ideal" aligners were constructed. The arch width and length between pairs of reference landmarks of the scanned CAs and corresponding dentition models were measured. Cone-beam computed tomography data and digital dental models were used for three-dimensional (3D) finite element analysis (FEA) modeling. Thirty-six scanned CA models with the corresponding 36 ideal CA models were constructed. One-way analysis of variance was used to determine the differences among deviation values at tooth level, and paired t-test was used to compare the displacements of teeth between the two group of CAs. RESULTS: All CAs were wider and shorter than the digital model from which they were constructed. In the scanned CA model group, significant stress was observed in the buccolingual area of the periodontal ligament on posterior teeth, and the corresponding displacements of teeth were also noted. Significantly larger coronal displacements were noted for the lateral incisor, the canine, the second premolar, and the first molar in the scanned CA group (P < .05). CONCLUSIONS: The general trend of ERD of thermoformed CAs was shown. This deformation may cause unforeseen tooth movements and negatively affect treatment outcomes.
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Aparatos Ortodóncicos Removibles , Ligamento Periodontal , Análisis de Elementos Finitos , Ligamento Periodontal/diagnóstico por imagen , Incisivo , Diente Molar , Técnicas de Movimiento DentalRESUMEN
INTRODUCTION: Finite element (FE) method has been used to calculate stress in the periodontal ligament (PDL), which is crucial in orthodontic tooth movement. The stress depends on the PDL material property, which varies significantly in previous studies. This study aimed to determine the effects of different PDL properties on stress in PDL using FE analysis. METHODS: A 3-dimensional FE model was created consisting of a maxillary canine, its surrounding PDL, and alveolar bone obtained from cone-beam computed tomography scans. One Newton of intrusion force was applied vertically to the crown. Then, the hydrostatic stress and the von Mises stress in the PDL were computed using different PDL material properties, including linear elastic, viscoelastic, hyperelastic, and fiber matrix. Young's modulus (E), used previously from 0.01 to 1000 MPa, and 3 Poisson's ratios, 0.28, 0.45, and 0.49, were simulated for the linear elastic model. RESULTS: The FE analyses showed consistent patterns of stress distribution. The high stresses are mostly concentrated at the apical area, except for the linear elastic models with high E (E >15 MPa). However, the magnitude varied significantly from -14.77 to -127.58 kPa among the analyzed patients. The E-stress relationship was not linear. The Poisson's ratio did not affect the stress distribution but significantly influenced the stress value. The hydrostatic stress varied from -14.61 to -95.48 kPa. CONCLUSIONS: Different PDL material properties in the FE modeling of dentition do not alter the stress distributions. However, the magnitudes of the stress significantly differ among the patients with the tested material properties.
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Ligamento Periodontal , Técnicas de Movimiento Dental , Ligamento Periodontal/diagnóstico por imagen , Análisis de Elementos Finitos , Técnicas de Movimiento Dental/métodos , Diente Canino/diagnóstico por imagen , Módulo de Elasticidad , Estrés Mecánico , Modelos Biológicos , Simulación por Computador , Fenómenos BiomecánicosRESUMEN
The accuracy of five failure criterions employed in the study of periodontal ligaments (PDL) during periodontal breakdown under orthodontic movements was assessed. Based on cone-beam computed tomography (CBCT) examinations, nine 3D models of the second lower premolar with intact periodontium were created and individually subjected to various levels of horizontal bone loss. 0.5 N of intrusion, extrusion, rotation, tipping, and translation was applied. A finite Elements Analysis (FEA) was performed, and stresses were quantitatively and qualitatively analyzed. In intact periodontium, Tresca and Von Mises (VM) stresses were lower than maximum physiological hydrostatic pressure (MHP), while maximum principal stress S1, minimum principal stress S3, and pressure were higher. In reduced periodontium, Tresca and VM stresses were lower than MHP for intrusion, extrusion, and the apical third of the periodontal ligament for the other movements. 0.5 N of rotation, translation and tipping induced cervical third stress exceeding MHP. Only Tresca (quantitatively more accurate) and VM are adequate for the study of PDL (resemblance to ductile), being qualitatively similar. A 0.5 N force seems safe in the intact periodontium, and for intrusion and extrusion up to 8 mm bone loss. The amount of force should be reduced to 0.1-0.2 N for rotation, 0.15-0.3 N for translation and 0.2-0.4 N for tipping in 4-8 mm periodontal breakdown. S1, S3, and pressure criteria provided only qualitative results.
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Ligamento Periodontal , Técnicas de Movimiento Dental , Simulación por Computador , Análisis de Elementos Finitos , Modelos Biológicos , Ligamento Periodontal/diagnóstico por imagen , Ligamento Periodontal/fisiología , Periodoncio , Estrés Mecánico , Técnicas de Movimiento Dental/métodosRESUMEN
BACKGROUND AND OBJECTIVE: During orthodontic treatment, the figure-of-eight ligature and the physiological occlusion play an important role in providing anchorage effects. However, their effects on reaction forces of tooth and stress state in periodontal ligament (PDL) have not been quantitatively evaluated yet. In this study, we presented a finite element analysis process for simulating posterior molar ligature and direct occlusion during orthodontics in order to quantitatively assess their anchorage effects. METHODS: A high precision 3D biomechanical model containing upper and lower teeth, PDL, brackets and archwire was generated from the images of computed tomographic scan and sophisticated modelling procedures. The orthodontic treatment of closing the extraction gap was simulated via the finite element method to evaluate the biomechanical response of the molars under the conditions with or without ligation. The simulations were divided into experimental and control groups. In the experimental group, orthodontic force of 1 N was first applied, then direct occlusal forces of 3 and 10 N were applied on each opposite tooth. While in the control group, occlusal forces were applied without orthodontic treatment. The tooth displacement, the stress state in the PDL and the directions of the resultant forces on each tooth were evaluated. RESULTS: In the case of molars ligated, the maximum hydrostatic stress in the molars' PDL decreases by 60%. When an initial tooth displacement of several microns occurs in response to an orthodontic force, the direction of the occlusal force changes simultaneously. Even a moderate occlusal force (3 N per tooth) can almost completely offset the mesial forces on the maxillary teeth, thus to provide effective anchorage effect for the orthodontics. CONCLUSIONS: The proposed method is effective for simulating ligation and direct occlusion. Figure-of-eight ligature can effectively disperse orthodontic forces on the posterior teeth, while a good original occlusal relationship provides considerable anchorage effects in orthodontics.
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Técnicas de Movimiento Dental , Diente , Análisis de Elementos Finitos , Técnicas de Movimiento Dental/métodos , Diente Molar/diagnóstico por imagen , Diente Molar/cirugía , Diente Molar/fisiología , Ligamento Periodontal/diagnóstico por imagen , Fenómenos Biomecánicos , Estrés MecánicoRESUMEN
The periodontal ligament (PDL) provides support, proprioception, nutrition, and protection within the tooth-PDL-bone complex (TPBC). While understanding the mechanical behavior of the PDL is critical, current research has inferred PDL mechanics from finite element models, from experimental measures on complete TPBCs, or through direct measurement of isolated PDL sections. Here, transducers are used in an attempt to quantify ex vivo PDL strain. In-fiber Bragg grating (FBG) sensors are small flexible sensors that can be placed within an intact TPBC and yield repeatable strain measurements from within the PDL space. The objective of this study was to determine: 1) if the FBG strain measured from the PDL space of intact swine premolars ex vivo was equivalent to physical PDL strains estimated through finite element analysis and 2) if a change in FBG strain could be linearly related to a change in finite element strain under variable tooth displacement, applied to an intact swine TPBC. Experimentally, individual TPBCs were subjected to 2 displacements (n = 14). The location of the FBG was determined from representative micro-computed tomography images. From a linear elastic finite element model of a TPBC, the strain magnitudes at the sensor locations were recorded. An experimental ratio (i.e., FBG strain at the first displacement divided by the FBG strain at the second displacement) and a finite element ratio (i.e., finite element strain at the first displacement divided by the finite element strain at the second displacement) were calculated. A linear regression model indicated a statistically significant relationship between the experimental and finite element ratio (P = 0.017) with a correlation coefficient (R2) of 0.448. It was concluded that the FBG sensor could be used as a measure for a change in strain and thus could be implemented in applications where the mechanical properties of an intact PDL are monitored over time.
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Ligamento Periodontal , Porcinos , Animales , Ligamento Periodontal/diagnóstico por imagen , Microtomografía por Rayos X , Fenómenos Biomecánicos , Análisis de Elementos Finitos , Diente PremolarRESUMEN
INTRODUCTION: The functional interplay between cementum of the root and alveolar bone of the socket is tuned by a uniquely positioned 70-80 µm wide fibrous and lubricious ligament in a dentoalveolar joint (DAJ). In this study, structural and biomechanical properties of the DAJ, periodontal ligament space (PDL-space also known as the joint space), alveolar bone of the socket, and cementum of the tooth root that govern the biomechanics of a lipopolysaccharide (LPS)-affected DAJ were mapped both in space and time. METHODS: The hemi-maxillae from 20 rats (4 control at 6 weeks of age, 4 control and 4 LPS-affected at 12 weeks of age, 4 control and 4 LPS-affected at 16 weeks of age) were investigated using a hybrid technique; micro-X-ray computed tomography (5 µm resolution) in combination with biomechanical testing in situ. Temporal variations in bone and cementum volume fractions were evaluated. Trends in mineral apposition rates (MAR) in additional six Sprague Dawley rats (3 controls, 3 LPS-affected) were revealed by transforming spatial fluorochrome signals to functional growth rates (linearity factor - RW) of bone, dentin, and cementum using a fast Fourier transform on fluorochrome signals from 100-µm hemi-maxillae sections. RESULTS: An overall change in LPS-affected DAJ biomechanics (a 2.5-4.5X increase in tooth displacement and 2X tooth rotation at 6 weeks, no increase in displacement and a 7X increase in rotation at 12 weeks; 27% increase in bone effective strain at 6 weeks and 11% at 12 weeks relative to control) was associated with structural changes in the coronal regions of the DAJ (15% increase in PDL-space from 0 to 6 weeks but only 5% from 6 to 12 weeks compared to control). A significant increase (p < 0.05) in PDL-space between ligated and age-matched control was observed. The bone fraction of ligated at 12 weeks was significantly lower than its age-matched control, and no significant differences (p > 0.05) between groups were observed at 6 weeks. Cementum in the apical regions grew faster but nonlinearly (11% and 20% increase in cementum fraction (CF) at 6 and 12 weeks) compared to control. Alveolar bone revealed site-specific nonlinear growth with an overall increase in MAR (108.5 µm/week to 126.7 µm/week after LPS treatment) compared to dentin (28.3 µm/week in control vs. 26.1 µm/week in LPS-affected) and cementum (126.5 µm/week in control vs. 119.9 µm/week in LPS-affected). A significant increase in CF (p < 0.05) in ligated specimens was observed at 6 weeks of age. CONCLUSIONS: Anatomy-specific responses of cementum and bone to the mechano-chemo stimuli, and their collective temporal contribution to observed changes in PDL-space were perpetuated by altered tooth movement. Data highlight the "resilience" of DAJ function through the predominance of nonlinear growth response of cementum, changes in PDL-space, and bone architecture. Despite the significant differences in bone and cementum architectures, data provided insights into the reactionary effects of cementum as a built-in compensatory mechanism to reestablish functional competence of the DAJ. The spatial shifts in architectures of alveolar bone and cementum, and consequently ligament space, highlight adaptations farther away from the site of insult, which also is another novel insight from this study. These adaptations when correlated within the context of joint function (biomechanics) illustrate that they are indeed necessary to sustain DAJ function albeit being pathological.
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Cemento Dental , Lipopolisacáridos , Animales , Maxilar , Ligamento Periodontal/diagnóstico por imagen , Ratas , Ratas Sprague-DawleyRESUMEN
INTRODUCTION: This research aimed to assess qualitatively and quantitatively the overall stress in the periodontal ligament during gradual periodontal breakdown (0-8 mm) under orthodontic movements. Correlations between the applied forces, the level of bone loss, the decrease of force magnitude, and the increase of stress were also assessed. METHODS: On the basis of cone-beam computed tomography examinations (voxel size, 0.075 mm), nine 3-dimensional models of a mandibular second premolar with intact periodontium were created and then individually subjected to various levels of horizontal bone loss. Orthodontic forces (intrusion at 0.2 N; extrusion, rotation, and tipping at 0.6 N; translation at 1.2 N) were applied on the brackets. Finite elements analysis was performed, and von Mises (VM) stresses were quantitatively and qualitatively determined. RESULTS: Rotation and translation induced the highest stress apically and cervically, whereas intrusion determined the lowest. Apical stress was lower than cervical stress. In intact periodontium, VM stress was under maximum hydrostatic pressure (MHP) and maximum tolerable stress (MTS). In reduced periodontium, VM stress was lower apically than MHP, whereas cervically, the rotation, translation, and tipping exceeded MHP. CONCLUSIONS: A force of 1.2 N seemed safe to be used in the intact periodontium. Forces higher than 0.6 N could produce stresses exceeding MHP and MTS endangering the reduced periodontium. VM stress failure criterion (despite its limited use) seemed to be more adequate for accurate quantitative results. An overall correlation between the applied force, VM stress increase, and periodontal breakdown applicable to all 5 movements could not be established. This was possible only for individual movements.
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Ligamento Periodontal , Técnicas de Movimiento Dental , Simulación por Computador , Análisis de Elementos Finitos , Humanos , Modelos Biológicos , Ligamento Periodontal/diagnóstico por imagen , Estrés Mecánico , Técnicas de Movimiento Dental/efectos adversosRESUMEN
Gold nanocomplexes have been proposed as contrast agents for computerized tomography (CT) and cell tracking, which is especially useful in stem cell therapy. However, their potential for long-term in vivo cell detection is still unknown. This study proposes an optimized approach to labeling human periodontal ligament stem cells (hPDLSCs) with gold nanocomplexes to evaluate their detection with micro-CT after transplantation at four different rat tissues. The gold nanocomplexes of 0.05 mg mL-1 do not affect cell viability nor osteogenic differentiation capacity, but render fluorescent and radiopaque hPDLSCs. Excellent linear correlation with the number of labeled cells is shown over a wide range (r = 0.99, P < 0.01), with a detection limit of ≈1.2 × 103 cells/µL. In vivo, strong, and durable detection of transplanted labeled cells within 5 days at all investigated areas is seen by micro-CT and immunohistochemical assay. This approach confirms the potential of gold nanocomplexes in longitudinal in vivo cell tracking, which may facilitate their application in CT image-guided interventions commonly used in oromaxillofacial or systemic applications of stem cell therapy.
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Osteogénesis , Ligamento Periodontal , Animales , Diferenciación Celular , Células Cultivadas , Oro , Humanos , Ligamento Periodontal/diagnóstico por imagen , Ratas , Células Madre , Tomografía Computarizada por Rayos XRESUMEN
INTRODUCTION: Basic research in orthodontics is commonly conducted in rodents. However, experimental studies on orthodontic tooth movement (OTM) lack a standard method to examine OTM and periodontal changes. This study describes a unifying protocol for the analysis of OTM and associated bone microarchitectural changes in mice using microcomputed tomography (µCT). METHODS: Mice (10 animals/group) were divided into control and OTM groups. OTM was generated by anchoring a nickel-titanium closed-coil spring to the upper incisors to pull the upper left first molar. A third group of TNFαâ-/- mice was added since these are known to have slower OTM. Using µCT, we implemented and tested a number of methods to measure OTM distance and examine 3D bone morphometric parameters associated with OTM in mice. RESULTS: In total, we tested five methods to measure the OTM distance in mice. The results indicated that measuring the intermolar diastema, and assessing tooth movement relative to the anterior root of the zygomatic arch, displayed the lowest standard deviation and enabled optimal detection of intergroup differences. We also developed two protocols for µCT analysis of the periradicular bone that yielded no false-positive results. Our results revealed that including the width of the periodontal ligament rather than excluding it from the region of interest in mice detected more statistically significant differences in the morphometric parameters between the OTM and control sides and between WT and TNFαâ-/- mice despite more subtle differences. CONCLUSIONS: We, therefore, propose new guidelines for a standardized µCT-based method to analyse OTM and the extent of the periradicular bone structural changes in mice.
Asunto(s)
Osteoclastos , Técnicas de Movimiento Dental , Animales , Remodelación Ósea , Humanos , Ratones , Ligamento Periodontal/diagnóstico por imagen , Técnicas de Movimiento Dental/métodos , Microtomografía por Rayos XRESUMEN
The objective of the present study was to analyze the radiographic visibility of the periodontal ligament in completed root formation lower third molars in a sample of lower northern Thai population. Digital panoramic images from 800 patients with ages ranging from 16 to 26 years were used in this study. The visibility status of the periodontal ligament of lower third molars with completed root formation including apical closure was assessed. For each stage, the minimum age, maximum age, median, mean, and standard deviation were calculated. The minimum age found in stage 0 was 16.17 years in males and 17.00 years in females. Stage 1 was first achieved at the age of 16.17 years in males and 17.08 years in females. The earliest onset of stage 2 was 17.00 years in males and 18.17 years in females. The incidence of stage 3 was first observed at 19.17 years in males and 18.83 years in females. It may be concluded that the radiographic visibility of the periodontal ligament in lower third molars may be a useful approach in the dental age assessment in a Thai population. In case the periodontal ligament visibility is found to be in stage 2, it may be confirmed that the individual is at least 18 years of age.
Asunto(s)
Determinación de la Edad por los Dientes , Tercer Molar , Adolescente , Adulto , Femenino , Humanos , Masculino , Tercer Molar/diagnóstico por imagen , Ligamento Periodontal/diagnóstico por imagen , Radiografía Panorámica , Tailandia , Adulto JovenRESUMEN
OBJECTIVES: The present study aimed to compare two different models of orthodontic tooth movement (OTM) in rats by evaluating tooth movement efficiency and periodontal tissues remodelling. DESIGN: Fifteen animals were randomly distributed into 3 groups: control group (untreated); ligature appliance (LA) as experimental OTM using a closed coil spring fixed around maxillary first molar by steel ligature; occlusal appliance (OA) as experimental OTM using a closed coil spring attached on the occlusal surface of the maxillary first molar. After 15 days, all animals were euthanized, and the maxilla of each animal was collected for qPCR, micro-computed tomography, and histological analyses. RESULTS: Interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha gene expressions were significantly upregulated in the animals of the LA group as compared to the other groups. No significant difference was observed in tooth displacement between both methods. The LA group presented higher linear bone loss and lower values of bone volume fraction, bone mineral density, trabecular number and increased values of trabecular separation compared to the other groups. The birefringent collagen content in the tension side of the periodontal ligament contained significantly lower collagen content in the LA group than in the control group. Furthermore, on the pressure side, the collagen content was significantly lower in the LA and OA groups than in the control group. CONCLUSIONS: The OA group presented little or no deleterious effect on periodontal tissues compared to the LA group, suggesting its use may be more reliable for OTM induction in rats for 15 days.