X-ray microanalysis of elements in the masticatory muscle after paresis of the right masseter.

Muscle activity and function appear to be related to ionic concentrations in the muscle. We investigated whether muscle paresis induced by injection of Botulinum toxin A (Botox) in 16-week-old pigs over a 56-day period is associated with ionic changes in the affected muscles. Tissue samples were taken from the masseter, temporalis, medial pterygoid, and geniohyoid muscles by a standardized method and used for energy-dispersive x-ray microanalysis in an environmental scanning electron microscope. The largest increase in Na(+) was measured in the right and left sides of the masseter muscle in treated animals. Additionally, a significant elevation of Na(+) was measured in the anterior part of the temporalis muscle and in the pterygoid muscle (P < 0.05). In temporalis and pterygoid muscles, an increase in sulfur in both sides of treated pigs' heads was observed. Botox((R)) has an indirect impact on ion concentrations, resulting in changes in muscle functional capacity and adaptive compensation of paretic muscle function by other muscles.

Localization of keratan sulphate and chondroitin-6-sulphate on the anteriorly displaced human temporomandibular joint disc--histological and immunohistochemical analysis.

The aetiology of temporomandibular joint (TMJ) disc displacement has been investigated using human post-mortem material. Twenty TMJ specimens were used for a histological procedure with haematoxylin and eosin staining and orcein staining. Immunohistological analysis with chondroitin-6-sulphate and keratan sulphate was also carried out on the same specimens. Immunohistochemically, the specific localization of chondroitin-6-sulphate and keratan sulphate in the antero-medial parts of the anteriorly displaced deformed TMJ disc, which may be load-bearing areas, was observed. Orcein staining revealed that specific elastic fibres pulling the anterior band and stretching the disc continuously, may be an important factor in disc displacement. On the basis of these results, we were able to propose a significant new hypothesis for the aetiology of disc displacement.

Intermuscular and intramuscular differences in myosin heavy chain composition of the human masticatory muscles.

Among and within the human masticatory muscles a large number of anatomical differences exists indicating that different muscles and muscle portions are specialized for certain functions. In the present study we investigated whether such a specialization is also reflected by intermuscular and intramuscular differences in fibre type composition and fibre cross-sectional area. Fibre type compositions and fibre cross-sectional areas of masticatory muscles were determined in eight cadavers using monoclonal antibodies against myosin heavy chain (MyHC). The temporalis, masseter and pterygoid muscles could be characterized by a relatively large number of fibres containing more than one MyHC isoform (hybrid fibres). In these muscles a large number of fibres expressed MyHC-I, MyHC-fetal and MyHC-cardiac alpha. Furthermore, in these muscles type I fibres had larger cross-sectional areas than type II fibres. In contrast, the mylohyoid, geniohyoid and digastric muscle were characterized by less hybrid fibres, and by less fibres expressing MyHC-I, MyHC-fetal, and MyHC-cardiac alpha, and by more fibres expressing MyHC-IIA; the cross-sectional areas of type I and type II fibres in these muscles did not differ significantly. Compared to the masseter and pterygoid muscles, the temporalis had significantly larger fibres and a notably different fibre type composition. The mylohyoid, geniohyoid, and digastric muscles did not differ significantly in their MyHC composition and fibre cross-sectional areas. Also intramuscular differences in fibre type composition were present, i.e., a regionally higher proportion of MyHC type I fibres was found in the anterior temporalis, the deep masseter, and the anterior medial pterygoid muscle portions; furthermore, significant differences were found between the bellies of the digastric.

Myosin isoform composition of the human medial and lateral pterygoid muscles.

The medial and lateral pterygoid muscles are different in structure as well as in function. The medial pterygoid muscle is concentrically active during jaw closing, and the superior head of the lateral pterygoid muscle is eccentrically active during jaw closing, while its inferior head is concentrically active during jaw opening. Architecturally, the medial pterygoid can deliver higher forces than the lateral pterygoid. We investigated whether these differences are reflected in the myosin heavy-chain (MyHC) composition and the fiber cross-sectional area (f-csa) of these muscles. The pterygoid muscles from eight cadavers were investigated by means of monoclonal antibodies against different isoforms of MyHC. The proportions of pure MyHC type I fibers did not differ significantly among the muscles (32% in medial pterygoid, 34% in superior head, and 36% in the inferior head of the lateral pterygoid), nor did the total proportions of pure MyHC type IIA and IIX fibers (16% in medial pterygoid, 26% in the superior head, and 19% in the inferior head of the lateral pterygoid). The mean f-csa of type I fibers was 1315 microm2, which did not differ significantly among the muscles, and was significantly larger than the f-csa of type IIA fibers. The relative proportions of hybrid fibers, which expressed more than one MyHC isoform, were 52% in the medial pterygoid, 40% in the superior head, and 45% in the inferior head of the lateral pterygoid and did not differ significantly among the muscles. The most abundant hybrid fiber types found were fibers expressing MyHCs-cardiac alpha+IIA and MyHCs-cardiac alpha+I+IIA. Significant regional differences were found in the proportions of MyHC type I fibers in the medial pterygoid and in the inferior head of the lateral pterygoid. Although the form and function of the muscles are different, we conclude that this is not reflected in their myosin isoform composition.

Histamine-induced internalization of substance P receptors in myoepithelial cells of the guinea pig nasal glands.

Numerous substance P (SP) immunoreactive nerve fibers were located around submucosal glands in the guinea pig nasal mucosa. Since these SP positive nerve fibers were also positive for vasoactive intestinal polypeptide, and to a lessor extent for neuropeptide Y, they were presumed to be parasympathetic fibers. SP receptor positive structures were observed exclusively on the membrane of myoepithelial cells in normal nasal mucosa, suggesting that myoepithelial cells are targets of SP positive fibers. SP receptor-like immunoreactivity was observed associated with intracellular organella of myoepithelial cells 5 min after intranasal histamine challenge, which may indicate the molecular basis for histamine-induced nasal discharge.

A morphometric investigation of myotube formation in rabbit embryo medial pterygoid muscle.

To determine the times of the appearance of myoblasts, early myotubes, late myotubes, and myofibers, we studied a region between two aponeuroses of the medial pterygoid masticatory muscle in embryos of two strains of rabbits, without disturbing the normal innervation. The objectives of this study were to define the quantitative relations among these cells and to determine their kinetics statistically. We used Fauve de Bourgogne and New Zealand rabbit embryos on day 17, day 17 plus 12 hours, day 18, day 18 plus 12 hours, and days 20, 22, and 28 of gestation. Cell proliferation was studied with a light microscope, by means of counting methods. Similar development was observed in the two strains of rabbits. The numbers of myoblasts decreased as follows: (i) a marked decrease; (ii) a sudden cessation of the decrease, marked by a rebound at 18 days, and lasting less than 24 hours; and (iii) a plateau between embryonic days 22 and 28. The onset of reduction in the number of early myotubes coincided with the rebound of myoblasts. The number of late myotubes increased at the time of maximal early myotube density and during rebound of the myoblasts. Myofiber densities were similar to late myotube densities on day 22. We suggest that early myotubes are formed very gradually by fusion of myoblasts, and that the significant increase in the numbers of myoblasts corresponds to the second generation of myoblasts necessary for differentiation of late myotubes.