RESUMEN
The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin, kanamycin, amikacin, and tobramycin, and related impurities of these antibiotics can be separated by means of micellar electrokinetic chromatography (MEKC). Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for these antibiotics. The background electrolyte was composed of sodium tetraborate (100 mM), sodium deoxycholate (20 mM), and ß-cyclodextrin (15 mM) having a pH value of 10.0. This method is valid for evaluation of gentamicin, kanamycin, and tobramycin. It has to be adopted for amikacin, paromomycin, neomycin, and netilmicin.
Asunto(s)
Aminoglicósidos/aislamiento & purificación , Antibacterianos/aislamiento & purificación , Cromatografía Capilar Electrocinética Micelar/métodos , Amicacina/química , Amicacina/aislamiento & purificación , Aminoglicósidos/química , Antibacterianos/química , Kanamicina/química , Kanamicina/aislamiento & purificación , Micelas , Netilmicina/química , Netilmicina/aislamiento & purificación , Sisomicina/química , Sisomicina/aislamiento & purificación , Tobramicina/química , Tobramicina/aislamiento & purificación , beta-Ciclodextrinas/química , beta-Ciclodextrinas/aislamiento & purificaciónRESUMEN
An efficient and reproducible procedure was developed for extracting aminoglycosides from renal cortical tissue. It involves a double homogenization and two rinsings with trichloroacetic acid. A higher recovery is obtained compared with that of other previously reported methods.
