RESUMEN
Tape measure (TM) proteins are essential for the formation of long-tailed phages. TM protein assembly into tails requires the action of tail assembly chaperones (TACs). TACs (e.g. gpG and gpT of E. coli phage lambda) are usually produced in a short (TAC-N) and long form (TAC-NC) with the latter comprised of TAC-N with an additional C-terminal domain (TAC-C). TAC-NC is generally synthesized through a ribosomal frameshifting mechanism. TAC encoding genes have never been identified in the intensively studied Escherichia coli phage T4, or any related phages. Here, we have bioinformatically identified putative TAC encoding genes in diverse T4-like phage genomes. The frameshifting mechanism for producing TAC-NC appears to be conserved in several T4-like phage groups. However, the group including phage T4 itself likely employs a different strategy whereby TAC-N and TAC-NC are encoded by separate genes (26 and 51 in phage T4).
Asunto(s)
Bacteriófago T4/genética , Escherichia coli/virología , Genoma Viral , Chaperonas Moleculares/genética , Proteínas de la Cola de los Virus/química , Virión/genética , Secuencia de Aminoácidos , Bacterias/virología , Bacteriófago T4/metabolismo , Bacteriófago T4/ultraestructura , Biología Computacional/métodos , Secuencia Conservada , Sistema de Lectura Ribosómico , Chaperonas Moleculares/clasificación , Chaperonas Moleculares/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Proteínas de la Cola de los Virus/clasificación , Proteínas de la Cola de los Virus/genética , Proteínas de la Cola de los Virus/metabolismo , Virión/metabolismo , Virión/ultraestructura , Ensamble de Virus/genéticaRESUMEN
Here, we report the identification of a novel domain--GG (domain in KIAA1199, FAM3, POMGnT1 and Tmem2 proteins, with two well-conserved glycine residues), present in eukaryotic FAM3 superfamily (FAM3A, FAM3B, FAM3C and FAM3D), POMGnT1 (protein O-linked mannose beta-1,2-N-acetylglucosaminyltransferase), TEM2 proteins as well as phage gp35 proteins. GG domain has been revealed to be implicated in muscle-eye-brain disease and non-syndromic hearing loss. The presence of GG domain in Bacteriophage gp35 hinge connector of long tail fiber might reflect the horizontal gene transfer from organisms. And we proposed that GG domain might function as important structural element in phage LTF.