RESUMEN
Sonodynamic therapy (SDT) has been extensively studied as a new type of non-invasive treatment for mammary cancer. However, the poor water solubility and defective biocompatibility of sonosensitizers during SDT hinder the sonodynamic efficacy. Herein, a nanoplatform has been developed to achieve high efficient SDT against mammary cancer through the host-guest interaction of ß-cyclodextrin/5-(4-hydroxyphenyl)-10,15,20-triphenylporphyrin (ß-CD-TPP) and ferrocenecarboxylic acid/chitooligosaccharides (FC-COS). Moreover, the glucose oxidase (GOx) was loaded through electrostatic adsorption, which efficiently restricts the energy supply in tumor tissues, thus enhancing the therapeutic efficacy of SDT for tumors. Under optimal conditions, the entire system exhibited favorable water solubility, suitable particle size and viable biocompatibility. This facilitated the integration of the characteristics of starvation therapy and sonodynamic therapy, resulting in efficient inhibition of tumor growth with minimal side effects in vivo. This work may provide new insights into the application of natural oligosaccharides for construct multifunctional nanocarrier systems, which could optimize the design and development of sonodynamic therapy strategies and even combination therapy strategies.
Asunto(s)
Quitosano , Oligosacáridos , Especies Reactivas de Oxígeno , Terapia por Ultrasonido , Oligosacáridos/química , Oligosacáridos/farmacología , Animales , Quitosano/química , Quitosano/farmacología , Femenino , Especies Reactivas de Oxígeno/metabolismo , Ratones , Terapia por Ultrasonido/métodos , beta-Ciclodextrinas/química , beta-Ciclodextrinas/farmacología , Ratones Endogámicos BALB C , Línea Celular Tumoral , Glucosa Oxidasa/metabolismo , Glucosa Oxidasa/química , Nanopartículas/química , Quitina/química , Quitina/análogos & derivados , Quitina/farmacología , Humanos , Antineoplásicos/farmacología , Antineoplásicos/química , Compuestos Ferrosos/química , Compuestos Ferrosos/farmacología , Neoplasias de la Mama/terapia , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/patología , Metalocenos/química , Metalocenos/farmacología , Porfirinas/química , Porfirinas/farmacologíaRESUMEN
Chitosan is a natural polymer with numerous biomedical applications. The cellular activity of chitosan has been studied in various types of cancer, including melanoma, and indicates that these molecules can open new perspectives on antiproliferative action and anticancer therapy. This study analyzes how different chitosan conformations, such as α-chitosan (CH) or ß-oligochitosan (CO), with various degrees of deacetylation (DDA) and molar mass (MM), both in different concentrations and in CH-CO mixtures, influence the cellular processes of SK-MEL-28 melanocytes, to estimate the reactivity of these cells to the applied treatments. The in vitro evaluation was carried out, aiming at the cellular metabolism (MTT assay), cellular morphology, and chitinase-like glycoprotein YKL-40 expression. The in vitro effect of the CH-CO mixture application on melanocytes is obvious at low concentrations of α-chitosan/ß-oligochitosan (1:2 ratio), with the cell's response supporting the hypothesis that ß-oligo-chitosan amplifies the effect. This oligochitosan mixture, favored by the ß conformation and its small size, penetrates faster into the cells, being more reactive when interacting with some cellular components. Morphological effects expressed by the loss of cell adhesion and the depletion of YKL-40 synthesis are significant responses of melanocytes. ß-oligochitosan (1.5 kDa) induces an extension of cytophysiological effects and limits the cell viability compared to α-chitosan (400-900 kDa). Statistical analysis using multivariate techniques showed differences between the CH samples and CH-CO mixtures.
Asunto(s)
Quitina , Proteína 1 Similar a Quitinasa-3 , Quitosano , Melanocitos , Oligosacáridos , Quitosano/química , Quitosano/farmacología , Melanocitos/efectos de los fármacos , Melanocitos/metabolismo , Humanos , Quitina/análogos & derivados , Quitina/farmacología , Quitina/química , Oligosacáridos/farmacología , Proteína 1 Similar a Quitinasa-3/metabolismo , Supervivencia Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Línea Celular Tumoral , Melanoma/tratamiento farmacológico , Melanoma/metabolismo , Melanoma/patologíaRESUMEN
To promote the bioconversion of marine chitin waste into value-added products, we expressed a novel pH-stable Micromonospora aurantiaca-derived chitinase, MaChi1, in Escherichia coli and subsequently purified, characterized, and evaluated it for its chitin-converting capacity. Our results indicated that MaChi1 is of the glycoside hydrolase (GH) family 18 with a molecular weight of approximately 57 kDa, consisting of a GH18 catalytic domain and a cellulose-binding domain. We recorded its optimal activity at pH 5.0 and 55 °C. It exhibited excellent stability in a wide pH range of 3.0-10.0. Mg2+ (5 mM), and dithiothreitol (10 mM) significantly promoted MaChi1 activity. MaChi1 exhibited broad substrate specificity and hydrolyzed chitin, chitosan, cellulose, soluble starch, and N-acetyl chitooligosaccharides with polymerization degrees ranging from three to six. Moreover, MaChi1 exhibited an endo-type cleavage pattern, and it could efficiently convert colloidal chitin into N-acetyl-D-glucosamine (GlcNAc) and (GlcNAc)2 with yields of 227.2 and 505.9 mg/g chitin, respectively. Its high chitin-degrading capacity and exceptional pH tolerance makes it a promising tool with potential applications in chitin waste treatment and bioactive oligosaccharide production.
Asunto(s)
Quitina , Quitinasas , Micromonospora , Quitinasas/metabolismo , Quitinasas/química , Quitinasas/aislamiento & purificación , Quitinasas/genética , Quitina/análogos & derivados , Quitina/metabolismo , Quitina/química , Concentración de Iones de Hidrógeno , Especificidad por Sustrato , Micromonospora/enzimología , Micromonospora/genética , Hidrólisis , Escherichia coli/genética , Quitosano/química , Estabilidad de EnzimasAsunto(s)
Antibacterianos , Antiinflamatorios , Antioxidantes , Quitina , Periimplantitis , Antioxidantes/farmacología , Antioxidantes/química , Antibacterianos/farmacología , Antibacterianos/química , Periimplantitis/tratamiento farmacológico , Antiinflamatorios/farmacología , Antiinflamatorios/química , Quitina/química , Quitina/farmacología , Quitina/análogos & derivados , Humanos , Animales , Pruebas de Sensibilidad MicrobianaRESUMEN
Chitooligosaccharides, the hydrolysis products of chitin, have superior biological activities and application value to those of chitin itself; however, the ordered and highly crystalline structure of chitin renders its degradation by chitinase difficult. Herein, the effects of plasma-activated water (PAW) pre-treatment on the physicochemical properties, crystal structure, and enzymatic hydrolysis of chitin were investigated. The hydrolysis of PAW-pre-treated chitin (PAW activation time of 5 min) using chitinase from Vibrio harveyi (VhChit2) yielded 71 % more reducing sugar, compared with that from untreated chitin, with the degree of chitin hydrolysis increasing from 13 % without pre-treatment to 23 % post-treatment. Moreover, the amount of VhChit2 adsorbed by chitin increased from 41.7 to 58.2 mg/g. Fourier transform infrared spectrometry revealed that PAW could break the ß-1,4-glycosidic bonds of chitin (but had no effects on the hydrogen and amido bonds), thereby decreasing the molecular weight and crystallinity of the polysaccharide, which caused its structural damage and enhanced its enzymatic hydrolysis by chitinase. Consequently, PAW pre-treatment can be considered a simple, effective, and environmentally-friendly method for the biotransformation of chitin as its easier hydrolysis yields high-value products.
Asunto(s)
Quitina , Quitinasas , Peso Molecular , Vibrio , Agua , Quitinasas/química , Quitinasas/metabolismo , Quitina/química , Quitina/metabolismo , Quitina/análogos & derivados , Agua/química , Hidrólisis , Vibrio/enzimologíaRESUMEN
Bioproduction of diverse N-acetyl chitooligosaccharides from chitin is of great value. In the study, a novel GH family 18 bifunctional chitinase gene (PsChi82) from Paenibacillus shirakamiensis was identified, expressed and biochemically characterized. PsChi82 was most active at pH 5.0, and 55 °C, and displayed remarkable pH stability with the broad pH range of 3.0-12.0. It showed high chitosanase activity of 10.6 U mg-1 and diverse hydrolysis products of GlcNAc, (GlcNAc)2, GlcN-GlcNAc and (GlcN)2-GlcNAc, which may facilitate comprehensively understanding of structure-function relationships of N-acetyl COSs. Three engineered variants were then expressed and characterized. Among them, PsChi82-CBM26 possessed specific activity of 25.1 U mg-1 against colloidal chitin, which was 2.1 folds higher than that of PsChi82. The diverse N-acetyl COSs were subsequently produced by PsChi82-CBM26 with a sugar content of 23.2 g L-1. These excellent properties may make PsChi82-CBM26 potentially useful for N-acetyl COSs production in the food and chemical industries.
Asunto(s)
Proteínas Bacterianas , Quitina , Quitinasas , Quitosano , Oligosacáridos , Paenibacillus , Quitinasas/química , Quitinasas/genética , Quitinasas/metabolismo , Oligosacáridos/química , Oligosacáridos/metabolismo , Quitina/química , Quitina/análogos & derivados , Quitina/metabolismo , Quitosano/química , Quitosano/metabolismo , Paenibacillus/enzimología , Paenibacillus/genética , Paenibacillus/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Concentración de Iones de Hidrógeno , Estabilidad de Enzimas , Hidrólisis , Ingeniería de ProteínasRESUMEN
Injectable hydrogels fabricated from natural polymers have attracted increasing attentions for their potential in biomedical application owing to the biocompatibility and biodegradability. A new class of natural polymer based self-healing hydrogel is constructed through dynamic covalent bonds. The injectable self-healing hydrogels are fabricated by introducing alginate aldehyde to form Schiff base bonds with the chitin nanofibers. These hydrogels demonstrate excellent self-healing properties, injectability, and pH-responsive sol-gel transition behaviors. As a result, they can serve as carriers to allow an effective encapsulation of doxorubicin (DOX) for drug delivery. Furthermore, these hydrogels exhibit excellent biocompatibility and degradability in vitro and in vivo. The sustained release of DOX from the hydrogels effectively suppresses tumor growth in animal models without causing significant systemic toxicity, suggesting their potential application in anti-tumor therapies.
Asunto(s)
Alginatos , Antineoplásicos , Quitina , Doxorrubicina , Hidrogeles , Nanofibras , Quitina/química , Quitina/análogos & derivados , Alginatos/química , Nanofibras/química , Animales , Doxorrubicina/química , Doxorrubicina/farmacología , Hidrogeles/química , Antineoplásicos/farmacología , Antineoplásicos/química , Humanos , Ratones , Sistemas de Liberación de Medicamentos , Portadores de Fármacos/química , Liberación de Fármacos , Materiales Biocompatibles/química , Inyecciones , Línea Celular TumoralRESUMEN
A new conjugate, galloyl-oligochitosan nanoparticles (GOCNPs), was fabricated and used as nano-vehicle for effective and controlled delivery of propolis extract (PE) in the form of PE#GOCNPs, targeting improving its pharmaceutical potential. H-bonding interactions between the carboxyl, amino, and hydroxyl groups of the GOCNPs and PE resulted in successful encapsulation, with an entrapment efficacy of 97.3 %. The PE#GOCNPs formulation also exhibited excellent physicochemical stability and time-triggered drug release characteristics under physiological conditions. Furthermore, PE#GOCNPs showed significant activity against MCF-7 and HEPG2 carcinoma cells by scavenging free oxygen radicals and upregulating antioxidant enzymes. Additionally, PE#GOCNPs displayed anti-inflammatory properties by increasing IL10 and reducing pro-inflammatory cytokines more effectively than celecoxib. Furthermore, PE#GOCNPs reduced the expression of epidermal growth factor receptor (EGFR) and survivin genes. Furthermore, the encapsulated PE demonstrated significant activity in suppressing sonic hedgehog protein (SHH). The use of GOCNPs in combination with propolis presents a promising new strategy for chemotherapy with reduced toxicity and enhanced biocompatibility. This novel approach has the potential to revolutionize the field of chemotherapy. Future studies should focus on the application of the encapsulated PE in various cancer cell lines, distinct gene expression factors, and cell cycles.
Asunto(s)
Antioxidantes , Proliferación Celular , Quitina , Quitosano , Nanopartículas , Oligosacáridos , Própolis , Humanos , Própolis/química , Própolis/farmacología , Quitosano/química , Antioxidantes/farmacología , Antioxidantes/química , Nanopartículas/química , Oligosacáridos/química , Oligosacáridos/farmacología , Quitina/análogos & derivados , Quitina/química , Quitina/farmacología , Proliferación Celular/efectos de los fármacos , Células Hep G2 , Células MCF-7 , Liberación de Fármacos , Antineoplásicos/farmacología , Antineoplásicos/química , Portadores de Fármacos/química , Sistemas de Liberación de MedicamentosRESUMEN
Herein, we describe in first the application of squid pens for the preparation of pharmaceutical-grade oligochitosan hydrochloride with the physicochemical characteristics corresponding with the requirements of the European Pharmacopoeia. It is shown that the use of specific properties of squid pens as a source of parent chitosan allows preparing the product with a high yield at relatively moderate process conditions used for squid pens treatments and chitosan depolymerization.
Asunto(s)
Quitina , Quitosano , Decapodiformes , Oligosacáridos , Quitosano/química , Decapodiformes/química , Oligosacáridos/química , Oligosacáridos/síntesis química , Animales , Quitina/química , Quitina/análogos & derivadosRESUMEN
Chitosan is a cationic polysaccharide derived from chitin deacetylation. This polysaccharide and its oligosaccharides have many biological activities and can be used in several fields due to their favorable characteristics, such as biodegradability, biocompatibility, and nontoxicity. This review aims to explore the antifungal potential of chitosan and chitooligosaccharides along with the conditions used for the activity and mechanisms of action they use to kill fungal cells. The sources, chemical properties, and applications of chitosan and chitooligosaccharides are discussed in this review. It also addresses the threat fungi pose to human health and crop production and how these saccharides have proven to be effective against these microorganisms. The cellular processes triggered by chitosan and chitooligosaccharides in fungal cells, and prospects for their use as potential antifungal agents are also examined.
Asunto(s)
Antifúngicos , Quitosano , Hongos , Oligosacáridos , Quitosano/química , Quitosano/farmacología , Antifúngicos/farmacología , Antifúngicos/química , Antifúngicos/síntesis química , Oligosacáridos/química , Oligosacáridos/farmacología , Hongos/efectos de los fármacos , Humanos , Quitina/química , Quitina/farmacología , Quitina/análogos & derivados , Pruebas de Sensibilidad MicrobianaRESUMEN
BACKGROUND: As the major protein (approximately 36%) in rice bran, globulin exhibits excellent foaming and emulsifying properties, endowing its useful application as a foaming and emulsifying agent in the food industry. However, the low water solubility restricts its commercial potential in industrial applications. The present study aimed to improve this protein's processing and functional properties. RESULTS: A novel covalent complex was fabricated by a combination of the Maillard reaction and alkaline oxidation using rice bran globulin (RBG), chitooligosaccharide (C), quercetin (Que) and resveratrol (Res). The Maillard reaction improved the solubility, emulsifying and foaming properties of RBG. The resultant glycosylated protein was covalently bonded with quercetin and resveratrol to form a (RBG-C)-Que-Res complex. (RBG-C)-Que-Res exhibited higher thermal stability and antioxidant ability than the native protein, binary globulin-chitooligosaccharide or ternary globulin-chitooligosaccharide-polyphenol (only containing quercetin or resveratrol) conjugates. (RBG-C)-Que-Res exerted better cytoprotection against the generation of malondialdehyde and reactive oxygen species in HepG2 cells, which was associated with increased activities of antioxidative enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) through upregulated genes SOD1, CAT, GPX1 (i.e. gene for glutathione peroxidase-1), GCLM (i.e. gene for glutamate cysteine ligase modifier subunit), SLC1A11 (i.e. gene for solute carrier family 7, member 11) and SRXN1 (i.e. gene for sulfiredoxin-1). The anti-apoptotic effect of (RBG-C)-Que-Res was confirmed by the downregulation of caspase-3 and p53 and the upregulation of B-cell lymphoma-2 gene expression. CONCLUSION: The present study highlights the potential of (RBG-C)-Que-Res conjugates as functional ingredients in healthy foods. © 2024 Society of Chemical Industry.
Asunto(s)
Antioxidantes , Quitosano , Oligosacáridos , Oryza , Quercetina , Resveratrol , Humanos , Quercetina/química , Quercetina/análogos & derivados , Oryza/química , Oligosacáridos/química , Resveratrol/química , Resveratrol/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Quitosano/química , Células Hep G2 , Quitina/química , Quitina/análogos & derivados , Superóxido Dismutasa/metabolismo , Superóxido Dismutasa/genética , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Reacción de Maillard , Catalasa/metabolismo , Catalasa/genética , Glutatión Peroxidasa/metabolismo , Glutatión Peroxidasa/genéticaRESUMEN
The accumulation of methylglyoxal (MGO) produced in high-temperature processed foods and excessive production in the body contributes to intestinal barrier dysfunction. In this study, we investigated the effects of chitooligosaccharides (COSs) of different molecular weights (<1â¯kDa, 1-3â¯kDa, 3-5â¯kDa, 5-10â¯kDa, and >10â¯kDa) on MGO-induced intestinal barrier dysfunction. We investigated the effect of COSs on inhibiting intracellular MGO accumulation/MGO-derived AGEs production and regulating the receptor for AGE (RAGE)-mediated downstream protein expression, including proteins related to apoptosis and inflammation, intestinal barrier integrity, and paracellular permeability. Pretreatment with COSs ameliorated MGO-induced increased RAGE protein expression, activation of apoptotic cascade/inflammatory response, loss of intestinal epithelial barrier integrity, and increased paracellular permeability, ameliorating intestinal dysfunction through MGO scavenging. 1-3â¯kDa COSs most effectively ameliorated MGO-induced intestinal dysfunction. Our results suggest the potential of COSs in improving intestinal health by ameliorating intestinal barrier dysfunction by acting as an MGO scavenger and highlighting the need for the optimization of the molecular weight of COSs to optimize its protective effects.
Asunto(s)
Quitosano , Productos Finales de Glicación Avanzada , Mucosa Intestinal , Peso Molecular , Oligosacáridos , Piruvaldehído , Receptor para Productos Finales de Glicación Avanzada , Oligosacáridos/farmacología , Oligosacáridos/química , Productos Finales de Glicación Avanzada/metabolismo , Receptor para Productos Finales de Glicación Avanzada/metabolismo , Animales , Quitosano/farmacología , Quitosano/química , Mucosa Intestinal/efectos de los fármacos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Humanos , Intestinos/efectos de los fármacos , Intestinos/patología , Inflamación/tratamiento farmacológico , Inflamación/metabolismo , Inflamación/inducido químicamente , Apoptosis/efectos de los fármacos , Quitina/farmacología , Quitina/análogos & derivados , Quitina/química , Permeabilidad/efectos de los fármacosRESUMEN
Chitin, as the second abundant biopolymers on earth after cellulose, has been used for synthesis of hydrogels in a wide range of applications due to its biocompatibility, biodegradability and nontoxicity. This review aims to provide an overview of the latest developments in the preparation, properties and drug controlled release of chitin-based hydrogels. In the first part, the preparation of hydrogels from native chitin, nano chitin and chitin derivatives via physical or chemical procedures was discussed in detail. The properties of chitin-based hydrogels, including gel strength, swelling degree and smart response characteristics, were described in the second part. This review also introduced how chitin-based hydrogel as a drug controlled release carrier is affected by composition, pH, temperature, magnetic field, electric field and other factors. We hope this review can provide guidelines for the rational design of chitin-based hydrogels in drug delivery systems.
Asunto(s)
Quitina/química , Sistemas de Liberación de Medicamentos/métodos , Liberación de Fármacos , Hidrogeles/química , Animales , Materiales Biocompatibles/química , Celulosa/química , Fenómenos Químicos , Quitina/análogos & derivados , Preparaciones de Acción Retardada , Portadores de Fármacos/química , Concentración de Iones de Hidrógeno , Nanopartículas/química , Temperatura , Resistencia a la TracciónRESUMEN
As a natural polymer, chitin has excellent biological properties such as biodegradability and immunological, antibacterial, and wound-healing activities and has numerous applications in cosmetics, drug delivery, and pharmaceuticals. Organic polymer monoliths have also drawn significant attention, owing to their high permeability, large surface area, and high mechanical strength. They are usually applied to separation, ion exchange, catalysis, and chromatography. We have previously prepared cellulose monoliths using biopolymers; however, because chitin possesses amide groups on its side chain, it is superior to cellulose for further chemical modification and applications. However, the utilization of chitin is restricted by its insolubility in water and common organic solvents. In this study, for the first time, a monolith was prepared by chemical modification of chitin using a thermally induced phase separation (TIPS) method. First, we prepared dibutyrylchitin (DBC) as a starting polymer that is soluble in organic solvents. To prepare the monolith, DBC was dissolved completely in dimethyl sulfoxide (DMSO) while heating, and deionized water was added to the solution. It was then cooled at 20⯰C to form a monolith via phase separation. The porous morphology of the DBC monolith was altered by regulating the DBC concentration, DMSO/H2O ratio, and aging temperature. The DBC monolith was converted to a chitin monolith by the alkaline hydrolysis of butyryl ester. The successful hydrolysis of butyryl ester was confirmed by the disappearance of the peak at 1735â¯cm-1 in the FT-IR spectra, which is related to the ester moiety of DBC. The chitin monolith has the potential to be utilized under water flow for catalysis, metal capture from wastewater, dye sorption, and drug delivery systems.
Asunto(s)
Quitina/análogos & derivados , Quitina/química , Catálisis , Sistemas de Liberación de Medicamentos/métodos , Ésteres/química , Hidrólisis , Polímeros/química , Porosidad , Solubilidad , Solventes/química , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Temperatura , Aguas Residuales/química , Agua/químicaRESUMEN
Microbial colonization of catheter surfaces is responsible for most healthcare-associated infections. Quaternized chitin and chitosan have excellent antimicrobial and biocompatible properties and can be used to provide safe and prolonged protection for biomedical catheters. Herein, we prepared quaternized ß-chitin derivative (QC)- and quaternized chitosan derivative (QCS)-based antimicrobial surfaces. The quaternized polysaccharides modified TPU surfaces exhibited hydrophilicity, good biocompatibility. Among these, QCS2-modified TPU exhibited excellent antibacterial properties against Gram-positive and Gram-negative bacteria, and prevented the adherence of bacteria compared with pristine TPU. The antibacterial activity of QCS2-modified surfaces maintained for 8 weeks under the condition of immersion in serum. An in vivo subcutaneous implantation experiment revealed 99.87% reduction of bacteria and reduced expression of inflammation-related factors in the surrounding tissue five days after implantation with QCS2-modified TPU. Therefore, quaternized polysaccharide-modified surfaces have promising potential in preventing medical catheter-associated infections.
Asunto(s)
Antibacterianos/farmacología , Infecciones Relacionadas con Catéteres/prevención & control , Quitina/química , Quitina/farmacología , Animales , Adhesión Bacteriana/efectos de los fármacos , Materiales Biocompatibles/química , Catéteres/microbiología , Quitina/análogos & derivados , Quitosano/química , Quitosano/farmacología , Escherichia coli/efectos de los fármacos , Femenino , Interacciones Hidrofóbicas e Hidrofílicas , Ratones , Pruebas de Sensibilidad Microbiana/métodos , Poliuretanos/química , Staphylococcus aureus/efectos de los fármacosRESUMEN
Rapid and effective hemorrhage control is essential to reduce mortality following traumatic injuries. Herein we developed an organic solvent-free process to prepare carboxymethyl chitin microsphere (CMCHm) in an aqueous two-phase system through heating and freeze-drying. To further enhance the hemostatic performance of CMCHm, we loaded calcium ions and in-situ polymerized dopamine to get modified hemostatic microspheres CMCHm-Ca2+ and CMCHm-PDA, respectively. The size of these microspheres was mainly distributed between 50 µm and 150 µm, and the porous microstructure was observed by SEM. The data of in vitro degradation, cell cytotoxicity, and hemolysis test indicated good biocompatibility of these microspheres. Importantly, CMCHm-Ca2+ and CMCHm-PDA displayed better hemostatic performance compared with CMCHm and the positive controls Yunnan baiyao® and Quickclean®. Especially, the bleeding time was reduced to 59 s (CMCHm-Ca2+) and 45 s (CMCHm-PDA) in the femoral artery/vein cut model, respectively. All these demonstrate CMCHm-Ca2+ and CMCHm-PDA hold great potential for rapid hemostasis.
Asunto(s)
Quitina/análogos & derivados , Hemorragia/tratamiento farmacológico , Hemostasis/efectos de los fármacos , Hemostáticos/química , Microesferas , Animales , Coagulación Sanguínea/efectos de los fármacos , Línea Celular , Quitina/química , Quitina/farmacología , Dopamina/química , Dopamina/farmacología , Hemorragia/metabolismo , Hemostáticos/farmacología , Ratones , Porosidad , Ratas , Solventes/químicaRESUMEN
This study focuses on a commercial plant elicitor based on chitooligosaccharides (BIG®), which aids in rice plant growth and disease resistance to bacterial leaf blight (BLB). When the pathogen (Xoo) vigorously attacks rice that has suffered yield losses, it can cause damage in up to 20% of the plant. Furthermore, Xoo is a seed-borne pathogen that can survive in rice seeds for an extended period. In this study, when rice seeds were soaked and sprayed with BIG®, there was a significant increase in shoot and root length, as well as plant biomass. Furthermore, BIG®-treated rice plants showed a significant reduction in BLB severity of more than 33%. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) analysis was used to characterize BIG®'s mechanism in the chemical structure of rice leaves. The SR-FTIR results at 1650, 1735, and 1114 cm-1 indicated changes in biochemical components such as pectins, lignins, proteins, and celluloses. These findings demonstrated that commercial BIG® not only increased rice growth but also induced resistance to BLB. The drug's target enzyme, Xoo 1075 from Xanthomonas oryzae (PDB ID: 5CY8), was analyzed for its interactions with polymer ingredients, specifically chitooligosaccharides, to gain molecular insights down to the atomic level. The results are intriguing, with a strong binding of the chitooligosaccharide polymer with the drug target, revealing 10 hydrogen bonds between the protein and polymer. Overall, the computational analysis supported the experimentally demonstrated strong binding of chitooligosaccharides to the drug target.
Asunto(s)
Quitina/análogos & derivados , Resistencia a la Enfermedad/efectos de los fármacos , Oryza/microbiología , Enfermedades de las Plantas/microbiología , Xanthomonas/crecimiento & desarrollo , Quitina/química , Quitina/farmacología , Quitosano , OligosacáridosRESUMEN
Plant-parasitic nematodes cause severe economic losses annually which has been a persistent problem worldwide. As current nematicides are highly toxic, prone to drug resistance, and have poor stability, there is an urgent need to develop safe, efficient, and green strategies. Natural active polysaccharides such as chitin and chitosan with good biocompatibility and biodegradability and inducing plant disease resistance have attracted much attention, but their application is limited due to their poor solubility. Here, we prepared 6-oxychitin with good water solubility by introducing carboxylic acid groups based on retaining the original skeleton of chitin and evaluated its potential for nematode control. The results showed that 6-oxychitin is a better promoter of the nematicidal potential of Purpureocillium lilacinum than other water-soluble chitin derivatives. After treatment, the movement of J2s and egg hatching were obviously inhibited. Further plant experiments found that it can destroy the accumulation and invasion of nematodes, and has a growth-promoting effect. Therefore, 6-oxychitin has great application potential in the nematode control area.
Asunto(s)
Antinematodos/farmacología , Quitina/análogos & derivados , Hypocreales/química , Tylenchoidea/efectos de los fármacos , Animales , Antinematodos/química , Cucumis sativus/parasitología , Locomoción , Reproducción , Tylenchoidea/patogenicidad , Tylenchoidea/fisiologíaRESUMEN
Some food components can regulate the intestinal barrier function. Herein, the effect of transglutaminase-type oligochitosan glycation on caseinate hydrolysate for its ability to maintain intestinal epithelial integrity and the tight junction (TJ) structure was investigated by assessing and comparing the bioactivities of glycated caseinate hydrolysate and caseinate hydrolysate against the lipopolysaccharide-induced barrier damage in the model cells (rat intestinal epithelial IEC-6 cells). The results from liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis demonstrated that oligochitosan glycation occurred at the Gln residues of α-S1-casein and α-S2-casein. The two hydrolysates retarded the lipopolysaccharide cytotoxicity toward IEC-6 cells and enhanced the barrier integrity by increasing the transepithelial electrical resistance or decreasing the paracellular permeability. In addition, these two hydrolysates could upregulate both mRNA and protein expression of three TJ proteins in IEC-6 cells. More importantly, the glycated caseinate hydrolysate had higher potential than caseinate hydrolysate to protect IEC-6 cells against the lipopolysaccharide-induced barrier damage, suggesting that the transglutaminase-mediated oligochitosan glycation of proteins is a useful approach to enforce protein biofunctions in the intestine.
Asunto(s)
Caseínas , Mucosa Intestinal , Lipopolisacáridos , Transglutaminasas , Animales , Quitina/análogos & derivados , Quitosano , Cromatografía Liquida , Células Epiteliales , Oligosacáridos , Permeabilidad , Ratas , Espectrometría de Masas en Tándem , Uniones Estrechas , Transglutaminasas/genéticaRESUMEN
Current challenge of using cytokines is its poor distribution and systemic side effects. To avoid this issue, we prepared the tumor-targeted and microenvironment-responsive nanocarriers (TRN), which were consisted of α-tocopheryl succinate (α-TOS) loaded mesoporous silica nanoparticles as cores, and surface-modified by thioketal-linkage, electrostatically coated with carboxymethyl chitin, and further anchored glucose-regulated protein 78-binding peptide as shells for encapsulating IL-12. TRN showed a size of 260 nm after encapsulated IL-12 and α-TOS with loading content of 0.0206% and 7.21%, respectively, and exhibited good biocompatibility to 4 T1 cells and macrophages. Moreover, IL-12/α-TOS loaded TRN displayed obvious anti-tumor efficacy on BALB/c nude mice bearing 4 T1 tumors, which was derived from promoted targeting to tumor tissue, endocytosed by macrophages and locally release IL-12 to subsequently repolarize tumor-associated macrophages into tumoricidal M1 phenotype with reduced side effects. The nanosystem exhibited as a promising strategy with functional conversion of macrophages in tumor microenvironment for anti-tumor therapy.