RESUMEN
Acupuncture reduces pain by activating specific areas called acupoints on the patient's body. When these acupoints are fully activated, sensations of soreness, numbness, fullness, or heaviness called De qi or Te qi are felt by clinicians and patients. There are two kinds of acupuncture, manual acupuncture and electroacupuncture (EA). Compared with non-acupoints, acupoints are easily activated on the basis of their special composition of blood vessels, mast cells, and nerve fibers that mediate the acupuncture signals. In the spinal cord, EA can inhibit glial cell activation by down-regulating the chemokine CX3CL1 and increasing the anti-inflammatory cytokine interleukin-10. This inhibits P38 mitogen-activated protein kinase and extracellular signal-regulated kinase pathways, which are associated with microglial activation of the C-Jun N-terminal kinase signaling pathway and subsequent astrocyte activation. The inactivation of spinal microglia and astrocytes mediates the immediate and long-term analgesic effects of EA, respectively. A variety of pain-related substances released by glial cells such as the proinflammatory cytokines tumor necrosis factor [Formula: see text], interleukin-1[Formula: see text], interleukin-6, and prostaglandins such as prostaglandins E2 can also be reduced. The descending pain modulation system in the brain, including the anterior cingulated cortex, the periaqueductal gray, and the rostral ventromedial medulla, plays an important role in EA analgesia. Multiple transmitters and modulators, including endogenous opioids, cholecystokinin octapeptide, 5-hydroxytryptamine, glutamate, noradrenalin, dopamine, [Formula: see text]-aminobutyric acid, acetylcholine, and orexin A, are involved in acupuncture analgesia. Finally, the "Acupuncture [Formula: see text]" strategy is introduced to help clinicians achieve better analgesic effects, and a newly reported acupuncture method called acupoint catgut embedding, which injects sutures made of absorbable materials at acupoints to achieve long-term effects, is discussed.
Asunto(s)
Analgesia por Acupuntura , Electroacupuntura , Neurotransmisores/fisiología , Analgesia por Acupuntura/métodos , Puntos de Acupuntura , Hormona Adrenocorticotrópica/fisiología , Animales , Encéfalo/irrigación sanguínea , Encéfalo/diagnóstico por imagen , Encéfalo/fisiología , Quimiocina CX3CL1/metabolismo , Citocinas/metabolismo , Dopamina/fisiología , Ácido Glutámico/fisiología , Hemodinámica , Humanos , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Sistema de Señalización de MAP Quinasas/fisiología , Neuroglía/fisiología , Norepinefrina/fisiología , Péptidos Opioides/fisiología , Serotonina/fisiología , Sincalida/fisiología , Médula Espinal/citología , Ácido gamma-Aminobutírico/fisiología , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Recently, we reported that non-sulfated cholecystokinin-8 (NS CCK-8) reduces food intake by cholecystokinin-B receptors (CCK-BR). To examine a possible site of action for this peptide, and based on the fact that both NS CCK-8 and CCK-BR are found centrally and peripherally, in the current study we hypothesized that NS CCK-8 increases Fos-like immunoreactivity (Fos-LI, a neuronal activation marker) in the dorsal vagal complex (DVC) of the hindbrain and the myenteric and submucosal plexuses of the small intestine. We found that intraperitoneal NS CCK-8 (0.5â¯nmol/kg) increases Fos-LI in the DVC, the myenteric and the submucosal plexuses of the duodenum and the myenteric plexus of the jejunum. The findings suggest, but does not prove, a potential role for the DVC and the enteric neurons in the feeding responses evoked by NS CCK-8.
Asunto(s)
Conducta Alimentaria/fisiología , Rombencéfalo/metabolismo , Sincalida/metabolismo , Animales , Encéfalo/metabolismo , Colecistoquinina , Sistema Nervioso Entérico/metabolismo , Intestino Delgado , Masculino , Plexo Mientérico/metabolismo , Neuronas/metabolismo , Fragmentos de Péptidos , Proteínas Proto-Oncogénicas c-fos/inmunología , Proteínas Proto-Oncogénicas c-fos/metabolismo , Ratas , Ratas Sprague-Dawley , Receptor de Colecistoquinina B/metabolismo , Sincalida/fisiología , Plexo Submucoso/metabolismo , Nervio Vago/metabolismoRESUMEN
Sulphated cholecystokinin octapeptide (CCK-8s) is involved in feeding regulation as an anorexigenic neuropeptide in vertebrates. In rodents, i.c.v. administration of CCK-8s has been shown to affect not only feeding behaviour, but also psychomotor activity. However, there is still no information available concerning the psychophysiological effects of CCK-8s in goldfish. Therefore, in the present study, we examined the effect of synthetic goldfish (gf) CCK-8s on psychomotor activity in this species. Intracerebroventricular administration of gfCCK-8s at 0.1, 0.5 and 2.5 pmol g-1 body weight (BW) did not affect swimming distance (locomotor activity). Because goldfish prefer the lower to the upper area of a tank, we used this as a preference test (upper/lower test) to assess anxiety-like behaviour. Intracerebroventricular administration of gfCCK-8s at 2.5 pmol g-1 BW shortened the time spent in the upper area. The action of gfCCK-8s mimicked that of FG-7142 (the central-type benzodiazepine receptor inverse agonist, an anxiogenic agent) at 5 and 10 pmol g-1 BW. The anxiogenic-like effect of gfCCK-8s was abolished by treatment with the CCK receptor antagonist proglumide at 50 pmol g-1 BW. We also investigated the localisation of CCK/gastrin-like immunoreactivity in the goldfish brain. CCK/gastrin-like immunoreactivity was observed in the anxiety-related regions (the nucleus habenularis and the interpeduncular nucleus). These data indicate that gfCCK-8s potently affects psychomotor activity in goldfish, and exerts an anxiogenic-like effect via the CCK receptor-signalling pathway.
Asunto(s)
Ansiedad/fisiopatología , Carpa Dorada/fisiología , Desempeño Psicomotor/fisiología , Sincalida/fisiología , Animales , Ansiedad/inducido químicamente , Carbolinas/administración & dosificación , Femenino , Inyecciones Intraventriculares , Locomoción/efectos de los fármacos , Masculino , Proglumida/administración & dosificación , Desempeño Psicomotor/efectos de los fármacos , Sincalida/administración & dosificaciónRESUMEN
Acute lung injury (ALI) is mainly characterized by diffusive injuries to lung epithelium and increased permeability of alveolar-capillary membranes caused by various factors, which leads to pulmonary edema and pulmonary closure. Lipopolysaccharide (LPS), which is the main component of the cell wall of gram-negative bacteria, is one of the most important factors causing pulmonary infection and ALI. More and more reports have indicated that hydrogen sulfide (H2S) is closely correlated with ALI and has anti-inflammation function, while the specific mechanism needs further investigation. Cholecystokinin-octapeptide (CCK-8), which is an important endogenous functional fragment belonging to CCK family, participates in anti-inflammatory and anti-endotoxic shock (ES). Whether CCK-8 plays important roles in curing ALI also needs further investigation. Herein, we concluded that CCK-8 alleviated the ALI induced by LPS via regulating the catalytic activity of cystathionine γ-lyase (CSE) and the formation of H2S. This work provides new medicine-designed target for clinical doctor to prevent and cure ALI.
Asunto(s)
Lesión Pulmonar Aguda/tratamiento farmacológico , Cistationina gamma-Liasa/fisiología , Sulfuro de Hidrógeno/farmacología , Sincalida/fisiología , Lesión Pulmonar Aguda/etiología , Animales , Antiinflamatorios/farmacología , Cistationina gamma-Liasa/metabolismo , Humanos , Inflamación/tratamiento farmacológico , Lipopolisacáridos/farmacología , Ratas , Choque Séptico/tratamiento farmacológicoRESUMEN
PURPOSE: MicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof. MATERIALS AND METHODS: MicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively. Luciferase reporter assay was used to confirm the target of miR-379. RESULTS: Platelet-derived growth factor-bb was found to promote cell proliferation and suppress miR-379 expression in VSMCs. Functional assays demonstrated that miR-379 inhibited cell proliferation, cell invasion, and migration. Flow cytometry results further showed that miR-379 induced apoptosis in VSMCs. TargetScan analysis and luciferase report assay confirmed that insulin-like growth factor-1 (IGF-1) 3'UTR is a direct target of miR-379, and mRNA and protein levels of miR-379 and IGF-1 were inversely correlated. Rescue experiments showed that enforced expression of IGF-1 sufficiently overcomes the inhibitory effect of miR-379 on cell proliferation, invasion, and migration in VSMCs. CONCLUSION: Our results suggest that miR-379 plays an important role in regulating VSMCs proliferation, invasion, and migration by targeting IGF-1.
Asunto(s)
Movimiento Celular/fisiología , Proliferación Celular/fisiología , Factor I del Crecimiento Similar a la Insulina/fisiología , MicroARNs/fisiología , Músculo Liso Vascular/citología , Proteínas Proto-Oncogénicas c-sis/fisiología , Apoptosis , Becaplermina , Humanos , Insulina , ARN Mensajero/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Sincalida/fisiología , Cicatrización de Heridas/fisiologíaRESUMEN
In order to elucidate the influences of CCK-8 on expressions of apoptosis-related genes, Bax, Bcl-2 and Caspase-3, of prefrontal cortex neurons in morphine-relapse rats, an effective, successful morphine-relapse-rat model using the conditioned place preference (CPP) under CCK-8 (0.01, 0.1 and 1.0µg, i.c.v) intervention was established. The prefrontal cortexes were made into slices with the cellular plasmas immunohistochemically stained. The expressions of Bax, Bcl-2, Caspase-3 of neurons were evaluated through their scores, and each corresponding ratio of Bax and Bcl-2 (Bax/Bcl-2) was also computed. The results showed that the expression of Bcl-2 was very weak and those of Bax and Caspase-3 were hardly seen in group normal saline; the expressions of Bax and Caspase-3 were strong and that of Bcl-2 was weak in group morphine and compared to group normal saline, there were significant differences (P<0.05); the expressions of Bax, Caspase-3 and the ratios of Bax/Bcl-2 have a gradually-decreased trend in the sequence of group 0.01µg, group 0.1µg and group 1.0µg, but the expression of Bcl-2 has an opposite trend in the same sequence, and compared to group morphine, there were significant differences (P<0.05) excluding group 0.01µg. So we draw a conclusion that CCK-8 (0.1 and 1.0µg, i.c.v) could protect neurons of prefrontal cortex through up-regulating the expression of Bcl-2, down-regulating those of Bax and Caspase-3 and reducing Bax/Bcl-2 ratio in the model of morphine-relapse rats.
Asunto(s)
Apoptosis/efectos de los fármacos , Apoptosis/genética , Morfina/administración & dosificación , Neuronas/metabolismo , Corteza Prefrontal/metabolismo , Sincalida/administración & dosificación , Animales , Caspasa 3/metabolismo , Condicionamiento Clásico/efectos de los fármacos , Condicionamiento Clásico/fisiología , Masculino , Neuronas/efectos de los fármacos , Corteza Prefrontal/efectos de los fármacos , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Ratas , Ratas Wistar , Recurrencia , Sincalida/fisiología , Proteína X Asociada a bcl-2/metabolismoRESUMEN
Peripheral anorectic hormones, such as glucagon-like peptide (GLP)-1, cholecystokinin (CCK)-8 and leptin, suppress food intake. The newly-identified anorectic neuropeptide, nesfatin-1, is synthesised in both peripheral tissues and the central nervous system, particularly by various nuclei in the hypothalamus and brainstem. In the present study, we examined the effects of i.p. administration of GLP-1 and CCK-8 and co-administrations of GLP-1 and leptin at subthreshold doses as confirmed by measurement of food intake, on nesfatin-1-immunoreactive (-IR) neurones in the hypothalamus and brainstem of rats by Fos immunohistochemistry. Intraperitoneal administration of GLP-1 (100 µg/kg) caused significant increases in the number of nesfatin-1-IR neurones expressing Fos-immunoreactivity in the supraoptic nucleus (SON), the area postrema (AP) and the nucleus tractus solitarii (NTS) but not in the paraventricular nucleus (PVN), the arcuate nucleus (ARC) or the lateral hypothalamic area (LHA). On the other hand, i.p. administration of CCK-8 (50 µg/kg) resulted in marked increases in the number of nesfatin-1-IR neurones expressing Fos-immunoreactivity in the SON, PVN, AP and NTS but not in the ARC or LHA. No differences in the percentage of nesfatin-1-IR neurones expressing Fos-immunoreactivity in the nuclei of the hypothalamus and brainstem were observed between rats treated with saline, GLP-1 (33 µg/kg) or leptin. However, co-administration of GLP-1 (33 µg/kg) and leptin resulted in significant increases in the number of nesfatin-1-IR neurones expressing Fos-immunoreactivity in the AP and the NTS. Furthermore, decreased food intake induced by GLP-1, CCK-8 and leptin was attenuated significantly by pretreatment with i.c.v. administration of antisense nesfatin-1. These results indicate that nesfatin-1-expressing neurones in the brainstem may play an important role in sensing peripheral levels of GLP-1 and leptin in addition to CCK-8, and also suppress food intake in rats.
Asunto(s)
Tronco Encefálico/metabolismo , Proteínas de Unión al Calcio/metabolismo , Proteínas de Unión al ADN/metabolismo , Conducta Alimentaria , Hormonas Gastrointestinales/fisiología , Hipotálamo/metabolismo , Leptina/fisiología , Proteínas del Tejido Nervioso/metabolismo , Neuronas/metabolismo , Animales , Ingestión de Alimentos , Hormonas Gastrointestinales/administración & dosificación , Péptido 1 Similar al Glucagón/administración & dosificación , Péptido 1 Similar al Glucagón/sangre , Péptido 1 Similar al Glucagón/fisiología , Inyecciones Intraperitoneales , Leptina/administración & dosificación , Leptina/sangre , Masculino , Nucleobindinas , Núcleo Hipotalámico Paraventricular/metabolismo , Ratas Wistar , Sincalida/administración & dosificación , Sincalida/fisiología , Núcleo Supraóptico/metabolismoRESUMEN
The study investigated the share of vagal input at infra- and supra-nodosal level and the contribution of CCK1 and CCK2 receptors to the cardiorespiratory responses produced by an intravenous injection of sulfated cholecystokinin octapeptide (CCK-8) in anesthetized rats. This compound administered intravenously at a dose of 50µg/kg induced short-lived decline in tidal volume and respiratory rate resulting in depression of minute ventilation. Midcervical vagotomy had no effect on CCK-8-evoked ventilatory changes, whereas supranodosal denervation abolished slowing down of breathing. Cardiovascular response to CCK challenge was characterized by a transient decrease followed by an augmentation in the mean blood pressure (MAP) in the intact animals. Vagotomy performed at both levels abrogated the declining phase of MAP. Blood pressure changes were associated with decreased heart rate present in all neural states. All cardiovascular and respiratory effects were antagonized by pre-treatment with devazepide-CCK1 receptors' antagonist, whereas CI988-antagonist of CCK2 receptors was ineffective. In conclusion, our results indicate that CCK-8 modulates slowing down of respiratory rhythm via CCK1 receptors located in the nodose ganglia (NG) and depresses tidal volume via central CCK1 dependent mechanism. CCK-8-evoked decline in blood pressure may be due to activation of vagal afferents, whereas pressor responses seem to be mediated by an activation of CCK1 receptors in the central nervous system. Bradycardia was probably induced by the direct action of CCK-8 on the heart pacemaker cells.
Asunto(s)
Presión Sanguínea , Frecuencia Cardíaca , Ganglio Nudoso/fisiología , Receptores de Colecistoquinina/fisiología , Respiración , Sincalida/fisiología , Administración Intravenosa , Animales , Presión Sanguínea/efectos de los fármacos , Devazepida/administración & dosificación , Frecuencia Cardíaca/efectos de los fármacos , Indoles/administración & dosificación , Masculino , Meglumina/administración & dosificación , Meglumina/análogos & derivados , Ratas , Ratas Wistar , Receptores de Colecistoquinina/antagonistas & inhibidores , Respiración/efectos de los fármacos , Frecuencia Respiratoria/efectos de los fármacos , Sincalida/administración & dosificación , Volumen de Ventilación Pulmonar/efectos de los fármacos , VagotomíaRESUMEN
BACKGROUND: Cholecystokinin (CCK), a gut hormone that is released during feeding, exerts gastrointestinal effects in part through vagal pathway. It is reported to be a potential trigger for increased postprandial visceral sensitivity in healthy subjects and, especially in patients with irritable bowel syndrome. NR2B-containing N-methyl-d-aspartate (NMDA) receptors in the central amygdala (CeA) participate in pain modulation. Systemically administered CCK activates the CeA-innervating neurons. Here, we investigated whether CCK modulation of visceral sensitivity is mediated through CeA NMDA-NR2B receptors and whether this modulation involves vagal pathway. METHODS: We first examined the visceromotor response (VMR) to colorectal distention (CRD) following i.p. injection of CCK octapeptide (CCK-8) in a rat model. Next, the NR2B antagonist ifenprodil and the NR2A antagonist NVP-AAM077 were microinjected into the CeA before systemic CCK injection. NR2B phosphorylation was detected by Western blot. To down-regulate NR2B gene expression, NR2B-specific small interfering RNA (siRNA) was delivered into CeA neurons by electroporation. In addition, the effects of functional deafferentation by perivagal application of capsaicin and pretreatment with the CCK1 receptor antagonist devazepide were investigated. KEY RESULTS: CCK-8 increased VMR to CRD in a dose-dependent manner. This effect was blunted by intra-CeA administration of ifenprodil (but not NVP-AAM077) and was accompanied by phosphorylation of NR2B subunits in the CeA. CCK failed to increase VMR to CRD in NR2B siRNA-treated rats. Perivagal capsaicin application and pretreatment with devazepide prevented CCK-induced pronociception and CeA NR2B phosphorylation. CONCLUSIONS & INFERENCES: The pronociception induced by systemic CCK, which is vagal afferent-dependent, requires activation of CeA NMDA-NR2B receptors.
Asunto(s)
Núcleo Amigdalino Central/fisiología , Nocicepción/fisiología , Receptores de N-Metil-D-Aspartato/fisiología , Sincalida/fisiología , Nervio Vago/fisiología , Dolor Visceral/fisiopatología , Músculos Abdominales/efectos de los fármacos , Músculos Abdominales/fisiopatología , Vías Aferentes/fisiología , Animales , Núcleo Amigdalino Central/efectos de los fármacos , Núcleo Amigdalino Central/metabolismo , Hiperalgesia/fisiopatología , Masculino , Nocicepción/efectos de los fármacos , Dimensión del Dolor , Fosforilación , Piperidinas/farmacología , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/agonistas , Receptores de N-Metil-D-Aspartato/metabolismo , Sincalida/administración & dosificación , Vagotomía , Dolor Visceral/metabolismoRESUMEN
Interstitial cells of Cajal (ICC) are pacemaker cells in the gastrointestinal (GI) tract and loss of ICC is associated with many GI motility disorders. Previous studies have shown that ICC have the capacity to regenerate or restore, and several growth factors are critical to their growth, maintenance or regeneration. The present study aimed to investigate the roles of interleukin-9 (IL-9) in the growth, maintenance and pacemaker functions of cultured ICC. Here, we report that IL-9 promotes proliferation of ICC, and culturing ICC with IL-9 enhances cholecystokinin-8-induced Ca²âº transients, which is probably caused by facilitating maintenance of ICC functions under culture condition. We also show co-localizations of cholecystokinin-1 receptor and IL-9 receptor with c-kit by double-immunohistochemical labeling. In conclusion, IL-9 can promote ICC growth and help maintain ICC functions; IL-9 probably performs its functions via IL-9 receptors on ICC.
Asunto(s)
Señalización del Calcio , Proliferación Celular , Colecistoquinina/fisiología , Interleucina-9/fisiología , Células Intersticiales de Cajal/fisiología , Animales , Anoctamina-1 , Células Cultivadas , Canales de Cloruro/metabolismo , Femenino , Masculino , Ratones Endogámicos BALB C , Antro Pilórico/citología , Receptores de Interleucina-9/metabolismo , Sincalida/fisiologíaRESUMEN
Cholecystokinin, a neuroactive peptide functioning as a neurotransmitter and neuromodulator in the central nervous system, mediates a number of processes and is implicated in neurological and psychiatric disorders such as Parkinson's disease, anxiety and schizophrenia. Striatum is one of the brain structures with the highest concentrations of CCK in the brain, rich in CCK receptors as well. The physiological effect of CCK on cholinergic interneurons, which are the major interneurons in striatum and the modulatory interactions which exist between dopamine, acetylcholine and cholecystokinin in this brain structure are still unclear. We studied the effect of cholecystokinin octapeptide (CCK-8) on the release of acetylcholine (ACh) from striatal slices of the rat brain. CCK-8 (0.01-0.1µM) showed no statistically significant effect on the basal but enhanced dose-dependently the electrically (2Hz)-evoked release of [(3)H]ACh. When slices were preperfused with 100µM sulpiride, a selective dopamine D(2) receptor antagonist, the CCK-8 (0.01µM) effect on electrically stimulated ACh release was increased nearly 2-fold. A similar increase was observed after depletion of endogenous dopamine (DA) from nigro-striatal dopaminergic neurons with 6-hydroxydopamine (6-OHDA) (2× 250µg/animal, i.c.v.). Furthermore in the presence of dopamine (100µM) or apomorphine (10µM), the prototypical DA receptor agonist, CCK-8 (0.01µM) failed to enhance the stimulation-evoked release of [(3)H]ACh. The D(2) receptor agonist quinpirol (1µM) abolished the CCK-8 effect on electrically stimulated ACh release as well. The increase in electrically induced [(3)H]ACh release produced by 0.01µM CCK-8 was antagonized by d,l loxiglumide (CR 1505), 10µM, a non-peptide CCK-A receptor antagonist and by Suc-Tyr-(OSO3)-Met-Gly-Trp-Met-Asp-ß-phenethyl-amide (GE-410), 1µM, a peptide CCK-A receptor antagonist. The antagonistic effect of GE-410 on the CCK-8-potentiated, electrically induced release of [(3)H]ACh was studied in striatum for the first time. CAM 1028 (10µM), a CCK-B receptor antagonist, also prevented the potentiating effect of CCK-8 (0.01µM) on electrically stimulated release of [(3)H]ACh. The presented results indicate that (i) CCK-8 is capable of increasing ACh elicited by field electrical stimulation in striatum; (ii) CCK-8 is more effective in its ACh-stimulating effect when dopaminergic activity in striatum is blocked i.e. CCK-8-facilitated release of electrically induced ACh from cholinergic interneurons in the striatum is under the inhibitory control of the tonic activity of dopamine from the nigrostriatal pathway; (iii) the enhancing effect of CCK-8 on electrically evoked ACh release is mediated through both CCK-A and CCK-B cholecystokinin receptors located most likely on the cell bodies of cholinergic interneurons in striatum.
Asunto(s)
Acetilcolina/metabolismo , Cuerpo Estriado/metabolismo , Neuronas Dopaminérgicas/fisiología , Receptor de Colecistoquinina A/fisiología , Receptor de Colecistoquinina B/fisiología , Sincalida/farmacología , Animales , Cuerpo Estriado/efectos de los fármacos , Dopamina/farmacología , Dopamina/fisiología , Neuronas Dopaminérgicas/efectos de los fármacos , Masculino , Técnicas de Cultivo de Órganos , Ratas , Ratas Wistar , Receptor de Colecistoquinina A/agonistas , Receptor de Colecistoquinina B/agonistas , Sincalida/fisiologíaRESUMEN
In anesthetized pigs gastrin-17 increased coronary blood flow through CCK1/CCK2 receptors and ß(2)-adrenoceptors-related nitric oxide (NO) release. Since the intracellular pathway has not been investigated the purpose of this study was to examine in coronary endothelial cells the CCK1/CCK2 receptors-related signaling involved in the effects of gastrin-17 on NO release. Gastrin-17 caused a concentration-dependent increase of NO production (17.3-62.6%; p<0.05), which was augmented by CCK1/CCK2 receptors agonists (p<0.05). The effect of gastrin-17 was amplified by the adenylyl-cyclase activator and ß(2)-adrenoceptors agonist (p<0.05), abolished by cAMP/PKA and ß(2)-adrenoceptors and CCK1/CCK2 receptors blockers, and reduced by PLC/PKC inhibitor. Finally, Western-blot revealed the preferential involvement of PKA vs. PKC as downstream effectors of CCK1/CCK2 receptors activation leading to Akt, ERK, p38 and endothelial NOS (eNOS) phosphorylation. In conclusion, in coronary endothelial cells, gastrin-17 induced eNOS-dependent NO production through CCK1/CCK2 receptors- and ß(2)-adrenoceptors-related pathway. The intracellular signaling involved a preferential PKA pathway over PKC.
Asunto(s)
Vasos Coronarios/citología , Células Endoteliales/metabolismo , Gastrinas/fisiología , Óxido Nítrico/metabolismo , Receptores de Colecistoquinina/metabolismo , Animales , Células Cultivadas , Células Endoteliales/efectos de los fármacos , Activación Enzimática , Gastrinas/farmacología , Sistema de Señalización de MAP Quinasas , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Donantes de Óxido Nítrico/farmacología , Óxido Nítrico Sintasa de Tipo III/metabolismo , Compuestos Nitrosos/farmacología , Pentagastrina/farmacología , Proteínas Proto-Oncogénicas c-akt/metabolismo , Sincalida/análogos & derivados , Sincalida/farmacología , Sincalida/fisiologíaRESUMEN
Cholecystokinin (CCK) is a potent regulator of visceral functions as a consequence of its actions on vago-vagal reflex circuit elements. This paper addresses three current controversies regarding the role of CCK to control gastric function via vago-vagal reflexes. Specifically: (a) whether CNS vs. peripheral (vagal afferent) receptors are dominant, (b) whether the long (58) vs. short (8) isoform is more potent and (c) whether nutritional status impacts the gain or even the direction of vago-vagal reflexes. Our in vivo recordings of physiologically identified gastric vagal motor neurones (gastric-DMN) involved in the gastric accommodation reflex (GAR) show unequivocally that: (a) receptors in the coeliac-portal circulation are more sensitive in amplifying gastric vagal reflexes; (b) in the periphery, CCK8 is more potent than CCK58; and (c) the nutritional status has a marginal effect on gastric reflex control. While the GAR reflex is more sensitive in the fasted rat, CCK amplifies this sensitivity. Thus, our results are in stark contrast to recent reports which have suggested that vago-vagal reflexes are inverted by the metabolic status of the animal and that this inversion could be mediated by CCK within the CNS.
Asunto(s)
Colecistoquinina/fisiología , Ayuno/fisiología , Neuronas Motoras/fisiología , Sincalida/fisiología , Nervio Vago/fisiología , Animales , Femenino , Masculino , Ratas , Ratas Long-Evans , Reflejo/fisiología , Estómago/inervación , Estómago/fisiologíaRESUMEN
This work was designed in order to gain an insight on the mechanisms by which antioxidants prevent pancreatic disorders. We have examined the properties of cinnamtannin B-1, which belongs to the class of polyphenols, against the effect of hydrogen peroxide (H(2)O(2)) in mouse pancreatic acinar cells. We have studied Ca(2+) mobilization, oxidative state, amylase secretion, and cell viability of cells treated with cinnamtannin B-1 in the presence of various concentrations of H(2)O(2). We found that H(2)O(2) (0.1-100 µM) increased CM-H(2)DCFDA-derived fluorescence, reflecting an increase in oxidation. Cinnamtannin B-1 (10 µM) reduced H(2)O(2)-induced oxidation of CM-H(2)DCFDA. CCK-8 induced oxidation of CM-H(2)DCFDA in a similar way to low micromolar concentrations of H(2)O(2), and cinnamtannin B-1 reduced the oxidant effect of CCK-8. In addition, H(2)O(2) induced a slow and progressive increase in intracellular free Ca(2+) concentration ([Ca(2+)](c)). Cinnamtannin B-1 reduced the effect of H(2)O(2) on [Ca(2+)](c), but only at the lower concentrations of the oxidant. H(2)O(2) inhibited amylase secretion in response to cholecystokinin, and cinnamtannin B-1 reduced the inhibitory action of H(2)O(2) on enzyme secretion. Finally, H(2)O(2) reduced cell viability, and the antioxidant protected acinar cells against H(2)O(2). In conclusion, the beneficial effects of cinnamtannin B-1 appear to be mediated by reducing the intracellular Ca(2+) overload and intracellular accumulation of digestive enzymes evoked by ROS, which is a common pathological precursor that mediates pancreatitis. Our results support the beneficial effect of natural antioxidants in the therapy against oxidative stress-derived deleterious effects on cellular physiology.
Asunto(s)
Células Acinares/efectos de los fármacos , Antioxidantes/farmacología , Peróxido de Hidrógeno/farmacología , Oxidantes/farmacología , Páncreas/citología , Proantocianidinas/farmacología , Sincalida/fisiología , Células Acinares/metabolismo , Amilasas/metabolismo , Animales , Señalización del Calcio/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Fluoresceínas/metabolismo , Colorantes Fluorescentes/metabolismo , Masculino , Ratones , Oxidación-ReducciónRESUMEN
Cholecystokinin octapeptide (CCK-8) is a typical brain-gut peptide that exerts a variety of physiological actions in both the peripheral and central nervous systems. Our laboratory has previously reported that CCK-8 produces immunoregulatory action through activating CCK receptor (CCK1R/CCK2R) expression on immune cell surfaces. In the present study, we investigated the effect of CCK-8 on immunoglobulin G1 (IgG1) production in lipopolysaccharide (LPS)-activated B cells in vitro. CCK-8 inhibited the proliferation and IgG1 mRNA expression of LPS-activated B cells and therefore inhibited IgG1 production. The mechanism may be associated with the regulation of CCK-8 on transcription factors Blimp1, Pax5, Xbp1 and Bcl6. CCK-8 inhibited the expression of Blimp1, while the effect on Pax5, Xbp1 and Bcl6 varied with time, suggesting that CCK-8 acted as a complex regulator of LPS-activated B cells. The inhibitory action of CCK-8 was mainly mediated through the CCK2R pathway. These studies indicate that CCK-8 attenuates humoral immune responses and acts as endogenous immune deactivators in autoimmune diseases.
Asunto(s)
Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Inmunoglobulina G/biosíntesis , Lipopolisacáridos/farmacología , Sincalida/farmacología , Animales , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Ensayo de Inmunoadsorción Enzimática , Femenino , Citometría de Flujo , Inmunoglobulina G/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Receptores de Colecistoquinina/antagonistas & inhibidores , Receptores de Colecistoquinina/fisiología , Sincalida/fisiología , Transcripción Genética/efectos de los fármacosRESUMEN
OBJECTIVE: Dental pulp stem cells (DPSCs) have been receiving more attentions recently as an important biomaterial for tissue engineering. Notch signalling plays a key role in regulating self-renewal and differentiation of a variety of cells. The objective of this study is to investigate the effects of Notch-Delta1 RNA interference (RNAi) on the proliferation and differentiation of human dental pulp stem cells in vitro. DESIGN: In the present study, we performed gene knockdown of Notch ligand Delta1 in DPSCs using lentivirus-mediated Delta1-RNAi. Changes of proliferation in DPSCs/Delta1-RNAi were examined by cell cycle analysis, Cell viability assay (CCK-8) and Western blot analysis of proliferating cell nuclear antigen (PCNA). Cells were cultured in odontoblast differentiation-inducing medium, and the differentiation of cells was detected with Alkaline phosphatase ALP activity assay, Alizarin red S staining, calcium concentration measurement, and Western blot analysis of Dentine sialophosphoprotein (DSPP). RESULTS: Lentivirus-mediated Delta1-RNAi stably knocked-down the expression of Delta1 and Notch signalling, and some of DPSCs/Delta1-RNAi displayed changes in morphology or DSPP expression. The growth rate of Delta1-deficient DPSCs was significantly suppressed as compared with wild type DPSCs and control lentivirus vector transfected DPSCs. Furthermore, the differentiating capability of DPSCs/Delta1-RNAi into odontoblasts is much higher than the two control groups. CONCLUSIONS: Notch signalling plays a crucial role in regulating self-renewal and differentiation in DPSCs. The deficient Notch signalling inhibits the self-renewal capacity of DPSCs and tends to induce DPSCs differentiation under odontoblast differentiation-inducing conditions. These findings suggested that DPSCs/Delta1-RNAi might be applicable to stem cell therapies and tooth tissue engineering.
Asunto(s)
Pulpa Dental/citología , Péptidos y Proteínas de Señalización Intracelular/fisiología , Proteínas de la Membrana/fisiología , Odontoblastos/citología , Receptores Notch/fisiología , Células Madre/fisiología , Fosfatasa Alcalina/metabolismo , Análisis de Varianza , Diferenciación Celular , Proliferación Celular , Supervivencia Celular , Células Cultivadas , Determinación de Punto Final , Proteínas de la Matriz Extracelular/biosíntesis , Técnicas de Silenciamiento del Gen , Humanos , Péptidos y Proteínas de Señalización Intracelular/antagonistas & inhibidores , Proteínas de la Membrana/antagonistas & inhibidores , Fosfoproteínas/biosíntesis , Antígeno Nuclear de Célula en Proliferación/biosíntesis , Interferencia de ARN , Sialoglicoproteínas/biosíntesis , Transducción de Señal , Sincalida/fisiología , Células Madre/citologíaRESUMEN
The purpose of this study was to evaluate the potential therapeutic effect of cholecystokinin octapeptide (CCK-8) on collagen-induced arthritis (CIA), an accepted murine experimental disease model with diverse histopathological features similar to human rheumatoid arthritis (RA). CIA was induced in DBA/1J mice by immunization with chicken collagen type II (CII). CCK-8 at different doses was intraperitoneally administered daily for 1 week. Mice treated with CCK-8 at doses of 5 and 10 nmol but not 1 nmol displayed much delayed onset of CIA and significantly lower incidence and decreased severity of arthritis. CCK-8 treatment significantly reduced the production of cytokines (IL-17, IL-23, IL-6 and TNF-α) and chemokines monocyte chemoattractant protein 1 in the joints of arthritic mice or in synovial cell culture supernatant, and increased the levels of IFN-γ and TGF-ß. T cells from CCK-8 treated mice proliferated much less, produced low level of IL-17 and high levels of IFN-γ and TGF-ß. Moreover, CCK-8 treated mice showed lower levels of CII-specific IgG, particularly that of IgG2a, in sera than those from control mice. These results indicate that CCK-8 is effective in suppressing both inflammatory and Th17 responses in CIA. CCK-8 may represent a new therapeutic modality for rheumatoid arthritis.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Artritis Experimental/patología , Artritis Experimental/prevención & control , Inmunosupresores/farmacología , Mediadores de Inflamación/fisiología , Sincalida/fisiología , Células Th17/inmunología , Animales , Artritis Experimental/inmunología , Células Cultivadas , Modelos Animales de Enfermedad , Femenino , Incidencia , Ratones , Ratones Endogámicos DBA , Índice de Severidad de la Enfermedad , Membrana Sinovial/inmunología , Membrana Sinovial/patología , Células Th17/patologíaRESUMEN
OBJECTIVE: To illustrate the existence of bile regurgitation under stress condition, and explore the possible effects and related mechanism of changes of cholecystokinin octapeptide (CCK-8) on stress-induced bile regurgitation in rats. METHODS: (1) Changes in plasma CCK-8 and gastric bile concentration were measured by using radioimmunoassay while simultaneously calculating gastric ulcer index and intragastric pH; (2) Each isolated gastric strips were suspended in a tissue chamber to record the contractile responses by polyphysiograph; (3) The responsiveness of gastric smooth muscle cells (SMCs) to sulfated cholecystokinin octapeptide (CCK-8S) were examined using fura-2-loaded microfluorimetric measurement of intracellular calcium concentration ([Ca(2+)] i); (4) The current of L-type calcium channels (I(CaL)) of SMCs were recorded by patch-clamp techniques. RESULTS: (1) Compared with the normal control, plasma CCK-8 [from (2.23 +/- 0.88) pmol/L to (10.80 +/- 3.82) pmol/L] and gastric bile concentration [from (37.93 +/- 23.76) micromol/L to (1316.00 +/- 197.36) micromol/L] significantly increased during the stress (P < 0.01) and both simultaneously reached the peak at the time point of 2 h after stress; ulcer index (from 0.62 +/- 0.23 to 32.01 +/- 16.11) and intragastric pH (from 1.06 +/- 1.20 to 5.29 +/- 1.25) apparently increased (P < 0.01); (2) Significant changes to CCK-8S were found in the mean contractile amplitude and frequency of circular muscle and longitudinal muscle of gastric antrum and pylorus; (3) CCK-8S-evoked significant increase in [Ca(2+)] i [from (65.8 +/- 7.4) nmol/L to (472.1 +/- 35.6) nmol/L, P < 0.01] could be suppressed by CCK-A receptor antagonist; whereas a small but significant increases were still elicited by CCK-8S under condition of the removal of extracellular calcium; (4) CCK-8S-intensified calcium current I(Ca-L) [from (-56.42 +/- 6.57) pA to (-88.54 +/- 5.71) pA, P < 0.01)] apparently inhibited by respective administration of nifedipine, Ca(2+)-ATPase inhibitors or calcium dependent chloride channel blocker (P < 0.01). CONCLUSIONS: Gastric mucosal damage induced by bile regurgitation is closely connected with gastric antrum and pylorus dysmotility evoked by CCK-8 during the stress. CCK-8S-evoked [Ca(2+)] i increase in gastric antrum and pylorus SMC depends on the release of intracellular calcium stores which activates L-type voltage-dependent calcium channels through the activation of calcium dependent chloride channels.