Spindle shaped Fe-Ni metal organic frameworks wrapped with f-MWCNTs for the efficacious sensing of tartrazine.

A facile hydrothermal route was employed for the synthesis of iron-nickel bimetal organic frameworks (Fe-Ni bi-MOFs) and composite with an acid functionalized multi-walled carbon nanotubes (Fe-Ni MOF/f-MWCNTs) for electrochemical detection of tartrazine. The as-prepared Fe-Ni MOF/f-MWCNTs was confirmed by the several physicochemical studies. A micro spindle shaped, highly porous, and crystalline Fe-Ni MOF/f-MWCNTs was noticed. The high sensitivity and stability of Fe-Ni MOF/f-MWCNTs/GCE modified electrode was analyzed. Due to its high porosity nature, the analyte molecule effectively gets adsorbed on the modified electrode and undergo electrochemical oxidation effectively. The modified electrode exhibits low limit of detection (LOD) and limit of quantification (LOQ) as 0.04 × 10-6 mol/L and 0.13 × 10-6 mol/L towards tartrazine. These results reveal the potential applications of Fe-Ni MOF/f-MWCNTs/GCE as modified electrode material for sensitive detection of tartrazine along with its robust reproducibility, stability, and effective sensing properties.

Multiplexed colorimetry collaborated with smartphone-based image analysis for simultaneous and fast visualization of dyes in both environmental and food samples.

In this work, a multiplexed colorimetric strategy was initiated for simultaneous and fast visualization of dyes using low-cost and easy-to-prepare indicator papers as sorbents. Response surface methodology (RSM) was employed to model statistically and optimize the process variables for dyes extraction and colorimetric assays. Multiplexed colorimetry was realized by virtue of synchronous color alignments from different dimensions of multiple dyes co-stained colorimetric cards under RSM-optimized conditions, and smartphone-based image analysis was subsequently performed from different modes to double-check the credibility of colorimetric assays. As concept-to-proof trials, simultaneous visualization of dyes in both beverages and simulated dye effluents was experimentally proved with results highly matched to HPLC or spiked amounts at RSM-predicted staining time as short as 50 s ∼3 min, giving LODs as low as 0.97 ± 0.22/0.18 ± 0.08 µg/mL (tartrazine/brilliant blue) for multiplexed colorimetry, which much lower than those obtained by single colorimetry. Since this is the first case to propose such a RSM-guided multiplexed colorimetric concept, it will provide a reference for engineering of other all-in-one devices which can realize synchronous visualization applications within limited experimental steps.

Azo dyes in the food industry: Features, classification, toxicity, alternatives, and regulation.

Azo dyes, including Tartrazine, Sunset Yellow, and Carmoisine, are added to foods to provide color, but they have no value with regard to nutrition, food preservation, or health benefits. Because of their availability, affordability, stability, and low cost, and because they provide intense coloration to the product without contributing unwanted flavors, the food industry often prefers to use synthetic azo dyes rather than natural colorants. Food dyes have been tested by regulatory agencies responsible for guaranteeing consumer safety. Nevertheless, the safety of these colorants remains controversial; they have been associated with adverse effects, particularly due to the reduction and cleavage of the azo bond. Here, we review the features, classification, regulation, toxicity, and alternatives to the use of azo dyes in food.

Tartrazine Modifies the Activity of DNMT and HDAC Genes-Is This a Link between Cancer and Neurological Disorders?

In recent years, artificial additives, especially synthetic food colorants, were found to demonstrate wider properties compared to their natural equivalents; however, their health impact is still not totally mapped. Our study aimed to determine the long-term (30 and 90 days) exposure effect of one of the commonly used artificial food colorants, tartrazine, on NMRI mice. The applied dose of tartrazine referred to the human equivalent dose for acceptable daily intake (ADI). Further, we evaluated its impact on the transcription of a range of epigenetic effectors, members of the DNA methyltransferase (DNMT) as well as histone deacetylase (HDAC) families. Following the exposure, organ biopsies were collected from the lungs, kidneys, liver, and spleen, and the gene expression levels were determined by real-time quantitative reverse transcription PCR (RT-qPCR). Our results demonstrated significant upregulation of genes in the tested organs in various patterns followed by the intake of tartrazine on ADI. Since DNMT and HDAC genes are involved in different steps of carcinogenesis, have roles in the development of neurological disorders and the effect of dose of everyday exposure is rarely studied, further investigation is warranted to study these possible associations.

Solvothermal synthesis of bifunctional carbon dots for tartrazine and Fe(III) detection from chamomile residue by ternary DES pretreatment.

A ternary deep eutectic solvent (DES) consisting of choline chloride, lactic acid, and urea in a molar ratio of 1:2:2 was used to pretreat chamomile residue, followed by carbon dots (CDs) preparation using a one-pot solvothermal method. The CDs prepared under the suitable conditions had a high quantum yield of 47.34% and could be used as a bifunctional fluorescent probe for the detection of tartrazine and Fe(III). The concentration of tartrazine or Fe(III) had a good linear relationship with the fluorescence intensity of CDs that the determination coefficient (R2) was 0.9957 and 0.9943, and the limit of detection (LOD) was 40 nM and 119 nM, respectively. After verifying the different fluorescence quenching mechanisms of CDs by these two substances, a quantitative analysis was performed on real samples with recoveries of 90.70%∼104.29%. Overall, this study provided a promising technology for chemical conversion from low-cost chamomile residue to attractive bifunctional fluorescent probe.

Sub-micro electrochemical recognition of carmoisine, sunset yellow, and tartrazine in fruit juices using P(ß-CD/Arg)/CysA-AuNPs/AuE.

An electrochemical sensor was designed to identify food colorants in juices. A green polymeric nanocomposite (beta-cyclodextrin/arginine) decorated with gold nanoparticles-capped cysteamine was fabricated on the surface of gold electrodes. Field emission-SEM and energy-dispersive X-ray spectroscopy showed the morphology and the presence of all elements related to all stages of the electrode modification. For three azo dyes (carmoisine, sunset yellow, and tartrazine), the analytical linear range was 10-8 to 10-4 M, with a low limit of quantification of about 1 nM. The engineered chemosensor showed suitable selectivity for analyzing candidate dyes in the presence of interfering agents. According to the scan rate results, the mass transport was controlled by diffusion, and the reaction on the chemosensor was electrochemically quasi-reversible. The results for different fruit juices confirmed this method's high potential application in detecting artificial color adulteration in food products.

Facile hydrothermal synthesis and purification of fluorescent carbon dots for food colorant tartrazine detection based on a dual-mode nanosensor.

Colorant tartrazine is widely used in the food industry, but its long-term and excessive consumption is harmful to human health. Therefore, it is necessary to establish a sensitive detection method for tartrazine. Blue fluorescent carbon dots with L-arginine and o-phenylenediamine as precursors, namely L-Arg/oPD-CDs, were prepared via the hydrothermal method. Then, L-Arg/oPD-CDs were further purified by dialysis, thin layer chromatography and column chromatography. A dual-mode nanosensor based on fluorescent and UV absorption was successfully developed. Excellent linear ranges of 0-5 µM and 10-50 µM were obtained with a low detection limit of 42.3 nM based on fluorescence. A good linear range of 0-50 µM was obtained with a low detection limit of 130.15 nM based on UV absorption. The quenching mechanism of tartrazine towards L-Arg/oPD-CDs fluorescence was the inner filter effect. In addition, a dual-mode nanosensor was used for tartrazine determination in millet, maize flour, carbonated drink, and sugar samples. This study provides new insight into the detection of tartrazine by applying a dual-mode nanosensor.

Carbon-paste electrode modified by ß-cyclodextrin as sensor for voltammetric determination of Tartrazine and Carmoisine from one drop.

For food quality control methods, low cost, speed, and simplicity are essential. Electrochemical methods can satisfy all of these requirements. In this paper, we propose a fast and simple voltammetric method using a carbon-paste electrode modified with ß-cyclodestrin for the determination of two common food azo dyes: Tartrazine and Carmoisine. To reduce the amount of sample required for analysis, in this work, we explored the prospect of another methodology similar to adsorption stripping voltammetry. The redox behavior of dyes, the influence of pH and scan rate on oxidation currents were investigated. Based on the results the scheme of oxidation of azo dyes was proposed. The use of the proposed approach in combination with the developed sensor makes it possible to determine Tartrazine and Carmoisine within their concentrations of 314-5024 ng/mL and 167-5340 ng/mL with calculation LOD 101 ng/mL and 60 ng/mL respectively. The proposed sensor was tested during analysis of model solutions and soft drinks and showed good results with high reproducibility.

An Electrochemical Sensor Based on Carbon Paper Modified with Graphite Powder for Sensitive Determination of Sunset Yellow and Tartrazine in Drinks.

The paper describes the development of an electrochemical sensor to be used for the determination of synthetic food colorants such as Sunset Yellow FCF (SY) and Tartrazine (TZ). The sensor is a carbon paper (CP) electrode, manufactured by using hot lamination technology and volume modified with fine-grained graphite powder (GrP). The sensor (GrP/CP) was characterized by scanning electron microscopy, energy dispersive spectrometry, electrochemical impedance analysis, cyclic, linear sweep and differential pulse voltammetry. The mechanism of SY and TZ electrochemical oxidation on GrP/CP was studied. The developed sensor has good electron transfer characteristics and low electron resistance, high sensitivity and selectivity. Applying the differential pulse mode, linear dynamic ranges of 0.005-1.0 µM and 0.02-7.5 µM with limits of detection of 0.78 nM and 8.2 nM for SY and TZ, respectively, were obtained. The sensor was used to detect SY and TZ in non-alcoholic and alcoholic drinks. The results obtained from drink analysis prove good reproducibility (RSD ≤ 0.072) and accuracy (recovery 96-104%).

Selective and sensitive detection of tartrazine in beverages by sulfur quantum dots with high fluorescence quantum yield.

In this work, sulfur quantum dots (TPA-SQDs) protected by terephthalic acid as a stabilizer were synthesized using a one-pot method. When excited at 310 nm, the synthesized TPA-SQDs solution emitted strong blue fluorescence at 428 nm, and the absolute quantum yield was as high as 85.99%. The proposed SQDs can be used as a fluorescent probe to specifically quench tartrazine (TZ), showing a good linear relationship (R2 = 0.996) at TZ concentrations of 0.1-20 µM, with a detection limit of 39 nM. By analysing the fluorescence lifetime, UV-Vis absorption spectrum and zeta potential of the assay system, it can be speculated that the fluorescence quenching mechanism of TZ on TPA-SQDs is the inner filter effect (IFE). The proposed method was applied to the detection of TZ in vitamin water and orange juice, and the results were consistent with the determination results by high-performance liquid chromatography. The recoveries and relative standard deviations were 93.2-102.6% and 1.34-2.88%, respectively, which provided an alternative method for the determination of TZ in beverages or other food samples.

Designing, characterization, and evaluation of chitosan-zinc selenide nanoparticles for visible-light-induced degradation of tartrazine and sunset yellow dyes.

Employing dyes in different industrial sectors has produced a serious threat to the environment and living organisms of water bodies and land. For the decontamination of such toxic dyes, efforts have been made to develop an efficient, feasible, and low maintenance processes. In this context, chitosan-zinc selenide (CS-ZnSe) nanoparticles were prepared through chemical reduction method as the efficient photocatalysts for the decontamination of toxic dyes through photocatalysis. Photocatalyst's synthesis was confirmed with the help of FTIR spectroscopy. XRD indicated the hexagonal crystal structure of the CS-ZnSe with a crystallite size of 12 nm. SEM micrographs showed the average nano photocatalyst size as 25 nm. EDX analysis was employed to determine the elemental composition of the CS-ZnSe. An excellent photocatalytic degradation efficiency for tartrazine and sunset yellow dyes was obtained using CS-ZnSe. The results showed a 98% and 97% degradation efficiency for tartrazine dye and sunset yellow (SY) dye at optimized conditions of time (3 h), pH (5), dye concentration (30 ppm), catalyst dosage (0.09 g and 0.01 g) , and at a temperature of 35 °C. Findings of the photocatalytic degradation process fitted well with first-order kinetics for both the dyes. Rate constant, 'K' value was found to be 0.001362 min-1 and 0.001257 min-1 for tartrazine and SY dyes, respectively. While value for (correlation coefficient, R2) was 0.99307 and 0.99277 for tartrazine and sunset yellow dyes, respectively. Recyclability of the photocatalyst was confirmed using it for consecutive cycles to degrade organic dyes. Results showed that the CH-ZnS possesses excellent efficiency in decontaminating organic dyes from industrial wastewater.

Three-dimensional porous reduced graphene oxide decorated with carbon quantum dots and platinum nanoparticles for highly selective determination of azo dye compound tartrazine.

In this work, an electrochemical sensor for the azo dye compound tartrazine (TRT) determination was proposed. A screen-printed carbon electrode (SPCE) was modified by depositing three-dimensional porous reduced graphene oxide decorated with carbon quantum dots and platinum nanoparticles (Pt/CQDs@rGO/SPCE). The resulting amount of TRT was observed by differential pulse voltammetry. Under optimal conditions, the sensor exhibited two wide linearities ranging from 0.01 to 1.57 µM and 1.57-9.3 µM with the reliability coefficient of determination of 0.991 and 0.992, respectively. The detection limit (LOD) was also estimated to be 7.93 nM. Moreover, the Pt/CQDs@rGO/SPCE suggested high selectivity in the presence of several interfering agents and azo dye compounds that have a similar structure. Additionally, the Pt/CQDs@rGO/SPCE revealed superior recovery values of about 96.5-101.6% for candy, 99.7-103.5% for soft drinks, 96.0-101.2% for jelly powder, and 98.0-103.0% for water samples. Furthermore, the fabricated sensor exhibits excellent selectivity, stability, reproducibility, and repeatability, indicating a great perspective in the monitoring of TRT. Therefore, it can be speculated that the proposed electrode could be effectively applied to determine TRT in food samples.

An electrochemical sensor based on hierarchical nickel oxide nanostructures doped with indium ions for voltammetric simultaneous determination of sunset yellow and tartrazine colorants in soft drink powders.

The current study was designed to develop a single-step and simple approach to effectively fabricate three-dimensional raspberry-like In3+/NiO hierarchical nanostructures (In3+/NiO RLHNSs) as a modifier, which was subsequently characterized by the techniques of X-ray diffraction (XRD), energy dispersive spectrometry (EDS) and field emission scanning electron microscopy (FE-SEM). The new prepared nano-modifier was practically used to co-detect electrochemically sunset yellow and tartrazine dyes. Potent sensitivity and acceptable selectivity were obtained for the produced In3+/NiO RLHNSs to co-detect both the food colorants, thus providing oxidation peaks in differential pulse voltammetry (DPV) with a peak potential separation of ca. 190 mV. The results showed a 5.14-fold and 8.07-fold increase in the electrochemical response of our modified electrode to sunset yellow and tartrazine, respectively, compared to the control (the unmodified electrode). Limits of detection of 2.7 and 3.1 nM were calculated for sunset yellow and tartrazine, respectively. The results from the interaction of common food additives showed satisfactory outcomes for the application of this method in determining sunset yellow and tartrazine in several beverage specimens. Other useful documentation was obtained for the production of portable food additive sensors.

Detection of tartrazine in fake saffron containing products by a sensitive optical nanosensor.

A fluorescent assay for the selective analysis of tartrazine was developed. Tartrazine is a health-threatening food additive commonly used as fake saffron. An optical nanosensor was fabricated based on molecular imprinting technique in which carbon dots (CDs) as fluorophores and tartrazine as a template molecule were embedded in molecularly imprinted polymer (MIP) matrix. The synthesized CDs embedded in MIP (CDs-MIP) was characterized by various methods. The fluorescence intensity of (CDs-MIP) was selectively quenched in the presence of tartrazine in comparison with other similar food color additives. The correlation between the quenching of CD-MIP and the concentration of tartrazine was used as an optical sensing for rapid detection of tartrazine in the range of 3.3-20.0 nM (1.8-10.7 µg L-1) with detection limit of 1.3 nM (0.70 µg L-1). Eventually, the designed nanosensor was successfully applied for tartrazine detection in foodstuffs such as fake saffron, saffron tea and saffron ice cream samples.

Rapid determination of seven synthetic dyes in casual snacks based on packed-fibers solid-phase extraction coupled with HPLC-DAD.

Based on packed-fiber solid-phase extraction and HPLC-DAD, a simple analytical method for the determination of seven synthetic dyes has been successfully developed. Polystyrene/polypyrrole (PS/PPy) fibers were obtained via electro-spinning of polystyrene skeletal nanofibers, followed by the oxidation with FeCl3 to trigger the polymerization of pyrrole and the deposition of polypyrrole coatings on PS fibrous skeleton fibers. The relationship between the extraction performance of the fibers and the electrospinning process at different humidities was investigated based on morphologic study and BET surface area. In the extraction process, purification, concentration, and desorption could be accomplished in one step. The established method exhibited good sensitivity, selectivity, reproducibility, and good efficiency for synthetic dyes in casual snacks (preserved fruit, flavored yogurt, and fruity hard candy) samples. With optimal conditions, the LODs (S/N = 3) were 2.4 to 21.09 ng mL-1, and linearities were acceptable in liquid matrix and solid matrices. The recoveries were 93.9-103.9%.

Development of a highly sensitive fluorescence method for tartrazine determination in food matrices based on carbon dots.

In this work, an ultrasensitive sensing system based on fluorescent carbon dots (CDs) was developed for the tartrazine (Tar) determination. The CDs were prepared via a simple one-pot hydrothermal method with m-phenylenediamine as the only precursor. The physical and chemical properties were in detail characterized by transmission electron microscopy (TEM), MALDI-TOF MS, UV-vis absorption and photoluminescence (PL) spectroscopy, elemental analysis, and Fourier transform infrared spectroscopy (FTIR). Upon exposure to Tar, the fluorescence of CDs was efficiently quenched via the dynamic interaction between CDs and Tar as well as the inner filter effect (IFE). With this information, the CDs were proposed as a fluorescence probe for Tar detection. It was found that CDs had high sensitivity and selectivity for Tar sensing, and the linear relationship was observed in the range of 0.01-25.0 µM with the corresponding detection limit (3σ/k) of 12.4 nM, which is much more sensitive than any of the existed CD-based sensing platform. The investigated sensing system was finally utilized for Tar sensing in various food matrices with a high degree of accuracy. The spiked recoveries were in a range of 96.4-105.2%, and the relative standard deviations (RSDs) were lower than 4.13%. This work highlights the great application prospects of CDs for Tar sensing in a rapid, simple, and sensitive way.

Green Synthesis of Fluorescent Carbon Dots from Elaeagnus angustifolia and its Application as Tartrazine Sensor.

This article has introduced and examined a novel and green approach for the very first time, which had been developed for the synthesis of carbon dots (CDs) and performed through the utilization of Elaeagnus angustifolia (E. A) as a natural carbon source. This straightforward procedure has been based upon a hydrothermal treatment with a quantum yield of 16.8% that had been designed to synthesize water-soluble CDs in one step and result in a satisfying fluorescence. Additionally, we have attempted to assess the sensing system that had been exerted through the usage of CDs for the detection of food colorant tartrazine, since they can function as a fluorescent sensor due to the interplay that occurs among tartrazine and CDs leading to the quenching of their fluorescence. The detection limit has been measured to be equaled to 0.086 µM (86 nM) and the linear range has been observed to be 0.47-234 µM. The proposed highly sensitive and simple method has exhibited an excellent selectivity and proved to be effectively applicable for distinguishing the tartrazine of real samples.

An electrochemical sensor for the determination of tartrazine based on CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode.

A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products. The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs. The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals. The optimum conditions were found at pH 7, 30 s, and 0.3 Vs-1. The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 µA µM-1. The limit of detection (LOD) and quantification obtained were 1.45 and 4.83 mg mL-1, respectively. The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%. The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.

NiFe2O4-rGO/ionic liquid modified carbon paste electrode: An amplified electrochemical sensitive sensor for determination of Sunset Yellow in the presence of Tartrazine and Allura Red.

In this paper, using a carbon paste electrode (CPE) modified with 1-ethyl-3-methylimidazolium chloride as an ionic liquid (IL) and NiFe2O4-rGO nanocomposite (IL/NiFe2O4/rGO/CPE), a sensitive and effective electrochemical sensor is applied to analyze Sunset Yellow. The X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX) and Fourier-transform infrared spectroscopy (FTIR) are employed to characterize the synthesized NiFe2O4-rGO nanocomposite. The oxidation peak currents of colorants were obtained by CV and DPV techniques; and as a result, the two linear ranges from 0.05 to 30 µM (R2 = 0.9939) and 30 to 500 µM (R2 = 0.9953) along with LOD of 0.03 µM for Sunset Yellow is obtained. The proposed sensor is successfully applied to determine the Sunset Yellow, Tartrazine and Allura Red in PBS (pH 3). The IL/NiFe2O4/rGO/CPE displays a high performance for analysis of these dyes in hair shampoo and an orange juice as real samples with acceptable results.

Electropolymerized melamine for simultaneous determination of nitrite and tartrazine.

Poly(melamine) (PMel) was synthesized via the electropolymerization of melamine monomer, which was then characterized by field-emission scanning electron microscopy (FESEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The possible polymerization mechanisms of melamine were also revealed by FT-IR spectroscopy and UV-Vis spectroscopy. Next, the PMel modified GCE (PMel/GCE) was used for the simultaneous determination of nitrite (NO2-) and tartrazine, and the parameters were optimized. The kinetic study showed that the electrochemical oxidation of nitrite and tartrazine at the surface of PMel/GCE is a typical surface-controlled electrode process. Under the optimun conditions, the developed sensor outperformed those previously reported, and it also exhibited high selectivity and reproducibility. Finally, the PMel/GCE was used for the simultaneous determination of nitrite and tartrazine in foodstuffs, and the results indicated that the proposed sensor could be a promising candidate for accurate determination of nitrite and tartrazine in real food samples.