RESUMEN
Molecular adjuvants are vaccine delivery vehicle to increase specific antigens effectiveness. Herein, we concentrated on IgG Fc, an effective molecular adjuvant, to develop novel pseudorabies virus (PRV) subunit vaccines. Two major protective antigen genes of PRV were constructed and linked into the mouse IgG Fc fragment. The gD, gD-IgG2aFc, gB and gB-IgG2aFc proteins were expressed using a baculovirus system. Mice intranasally immunized with gD-IgG2aFc or gB-IgG2aFc subunit vaccine exhibited significantly higher PRV-specific antibodies, neutralizing antibodies and intracellular cytokines than the mice intranasally immunized with gD or gB subunit vaccine. Moreover, no histopathological lesions were observed in mice immunized with gB-IgG2aFc subunit vaccine via histopathology examination. Further, the gB-IgG2aFc subunit vaccine was efficient for PRV infection compared with live attenuated vaccine. Overall, these results suggest that IgG2a Fc fragment, as a potential molecular adjuvant, fused with PRV antigen might be a promising and efficient PRV vaccine candidate.
Asunto(s)
Herpesvirus Suido 1/efectos de los fármacos , Fragmentos Fc de Inmunoglobulinas/biosíntesis , Vacunas contra la Seudorrabia/biosíntesis , Seudorrabia/prevención & control , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas del Envoltorio Viral/biosíntesis , Adyuvantes Inmunológicos/administración & dosificación , Adyuvantes Inmunológicos/biosíntesis , Adyuvantes Inmunológicos/genética , Animales , Anticuerpos Antivirales/biosíntesis , Baculoviridae/genética , Baculoviridae/metabolismo , Citocinas/genética , Citocinas/inmunología , Células Epiteliales/patología , Células Epiteliales/virología , Femenino , Glicoproteínas/administración & dosificación , Glicoproteínas/biosíntesis , Glicoproteínas/genética , Herpesvirus Suido 1/genética , Herpesvirus Suido 1/inmunología , Herpesvirus Suido 1/patogenicidad , Inmunización , Fragmentos Fc de Inmunoglobulinas/administración & dosificación , Fragmentos Fc de Inmunoglobulinas/genética , Inmunoglobulina G/administración & dosificación , Inmunoglobulina G/biosíntesis , Inmunoglobulina G/genética , Riñón/patología , Riñón/virología , Ratones , Ratones Endogámicos BALB C , Seudorrabia/inmunología , Seudorrabia/mortalidad , Seudorrabia/virología , Vacunas contra la Seudorrabia/administración & dosificación , Vacunas contra la Seudorrabia/genética , Proteínas Recombinantes de Fusión/administración & dosificación , Proteínas Recombinantes de Fusión/genética , Análisis de Supervivencia , Porcinos , Vacunas de Subunidad , Proteínas del Envoltorio Viral/administración & dosificación , Proteínas del Envoltorio Viral/genéticaRESUMEN
Pseudorabies (PR), foot-and-mouth disease (FMD), and porcine parvovirus disease are three important infectious diseases in swine worldwide. The gene-deleted pseudorabies virus (PRV) has been used as a live-viral vector to develop multivalent genetic engineering vaccine. In this study, a recombinant PRV, which could co-express protein precursor P1-2A of FMDV and VP2 protein of PPV, was constructed using PRV TK(-)/gE(-)/LacZ(+) mutant as the vector. After homologous recombination and plaque purification, recombinant virus PRV TK(-)/gE(-)/P1-2A-VP2 was acquired and identified. Immunogenicity, safety of the recombinant PRV and its protection against PRV were confirmed in a mouse model by indirect ELISA and serum neutralization test. The results show that the recombinant PRV is a candidate vaccine strain to develop a novel trivalent vaccine against PRV, FMDV and PPV in swine.
