Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 338
Filtrar
1.
J Infect Dev Ctries ; 18(6): 900-908, 2024 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-38990990

RESUMEN

INTRODUCTION: Vibrio parahaemolyticus is a common pathogen that can cause seafood-borne gastroenteritis in humans. We determined the prevalence and characteristics of V. parahaemolyticus isolated from clinical specimens and oysters in Thailand. METHODOLOGY: Isolates of V. parahaemolyticus from clinical specimens (n = 77) and oysters (n = 224) were identified by biochemical testing, polymerase chain reaction (PCR) assays, and serotyping. The toxin genes, antimicrobial resistance, and ß-lactamase production were determined. RESULTS: A total of 301 isolates were confirmed as V. parahaemolyticus by PCR using specific primers for the toxR gene. The majority of clinical isolates carried the tdh+/trh- genotype (82.1%), and one of each isolate was tdh-/trh+ and tdh+/trh+ genotypes. One isolate from oyster contained the tdh gene and another had the trh gene. Twenty-six serotypes were characterized among these isolates, and O3:K6 was the most common (37.7%), followed by OUT:KUT, and O4:K9. In 2010, most clinical and oyster isolates were susceptible to antibiotics, with the exception of ampicillin. In 2012, clinical isolates were not susceptible to cephalothin (52.4%), streptomycin (95.2%), amikacin (66.6%), kanamycin (61.9%), and erythromycin (95.2%), significantly more frequently than in 2010. More than 95% of isolates that were not susceptible to ampicillin produced ß-lactamase enzymes. CONCLUSIONS: We found toxin genes in two oyster isolates, and the clinical isolates that were initially determined to be resistant to several antibiotics. Toxin genes and antimicrobial susceptibility profiles of V. parahaemolyticus from seafood and environment should be continually monitored to determine the spread of toxin and antimicrobial resistance genes.


Asunto(s)
Ostreidae , Vibriosis , Vibrio parahaemolyticus , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/aislamiento & purificación , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/clasificación , Tailandia/epidemiología , Ostreidae/microbiología , Humanos , Animales , Vibriosis/microbiología , Vibriosis/epidemiología , beta-Lactamasas/genética , Antibacterianos/farmacología , Pruebas de Sensibilidad Microbiana , Serotipificación , Reacción en Cadena de la Polimerasa , Prevalencia , Genotipo , Farmacorresistencia Bacteriana/genética , Toxinas Bacterianas/genética , Masculino , Adulto , Femenino , Persona de Mediana Edad
2.
Microbiol Spectr ; 12(7): e0017524, 2024 Jul 02.
Artículo en Inglés | MEDLINE | ID: mdl-38832768

RESUMEN

Vibrio parahaemolyticus is a threat to human health and one of the leading bacterial causes of seafood-borne infection worldwide. This pathogen is autochtonous in the marine environment and is able to acquire antimicrobial resistance (AMR) mechanisms, which is a global concern. However, the emergence of AMR V. parahaemolyticus strains in seafood is still understudied, as interpretation criteria for this species for antimicrobial susceptibility tests are limited in the literature. In this study, we investigated the susceptibility profiles to clinically important antibiotics and the associated genetic determinants of V. parahaemolyticus isolates cultured from imported shrimps. Based on the analysis of the resistance phenotypes of 304 V. parahaemolyticus isolates, we have defined experimental epidemiological cutoff values (COWT) for 14/15 antibiotics tested. We observed that 19.1% of the bacterial isolates had acquired resistance to at least one antibiotic class. The highest number of resistance was associated with tetracycline (14.5% of the strains) and trimethoprim-sulfamethoxazole (3.6%). Moreover, seven strains were multidrug-resistant (MDR, resistant to at least three antibiotic classes). The most frequently identified genes in these strains were aph(3″)-Ib/aph(6)-Id (aminoglycoside resistance), sul2 (sulfonamide), tet(59) (tetracycline), and floR (chloramphenicol). The SXT/R391 family ICE and class 1 integron-integrase genes were detected by PCR in three and one MDR V. parahaemolyticus strains, respectively. Consequently, V. parahaemolyticus in seafood can act as a reservoir of AMR, constituting a health risk for the consumer.IMPORTANCEOur study on "Antimicrobial Resistance Profiles and Genetic Determinants of Vibrio parahaemolyticus Isolates from Imported Shrimps" addresses a critical gap in understanding the emergence of antimicrobial resistance (AMR) in this seafood-associated pathogen. Vibrio parahaemolyticus is a major cause of global seafood-borne infections, and our research reveals that 19.1% of isolates from imported shrimps display resistance to at least one antibiotic class, with multidrug resistance observed in seven strains. Importantly, we establish experimental epidemiological cutoff values for antibiotic susceptibility, providing valuable criteria specific to V. parahaemolyticus. Our findings underscore the potential risk to consumers, emphasizing the need for vigilant monitoring and intervention strategies. This study significantly contributes to the comprehension of AMR dynamics in V. parahaemolyticus, offering crucial insights for global public health. The dissemination of our research through Microbiology Spectrum ensures broad accessibility and impact within the scientific community and beyond.


Asunto(s)
Antibacterianos , Pruebas de Sensibilidad Microbiana , Alimentos Marinos , Vibrio parahaemolyticus , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/aislamiento & purificación , Vibrio parahaemolyticus/clasificación , Animales , Antibacterianos/farmacología , Alimentos Marinos/microbiología , Farmacorresistencia Bacteriana Múltiple/genética , Vibriosis/microbiología , Vibriosis/epidemiología , Penaeidae/microbiología , Humanos , Farmacorresistencia Bacteriana/genética
3.
J Agric Food Chem ; 72(25): 14337-14348, 2024 Jun 26.
Artículo en Inglés | MEDLINE | ID: mdl-38867141

RESUMEN

Thymol has efficient bactericidal activity against a variety of pathogenic bacteria, but the bactericidal mechanism against Vibrio parahemolyticus (V. parahemolyticus) has rarely been reported. In the current study, we investigated the bactericidal mechanism of thymol against V. parahemolyticus. The Results revealed that 150 µg/mL of thymol had 99.9% bactericidal activity on V. parahemolyticus. Intracellular bursts of reactive oxygen species (ROS), Fe2+accumulation, lipid peroxidation, and DNA breakage were checked by cell staining. The exogenous addition of H2O2 and catalase promoted and alleviated thymol-induced cell death to a certain extent, respectively, and the addition of the ferroptosis inhibitor Liproxstatin-1 also alleviated thymol-induced cell death, confirming that thymol induced Fenton-reaction-dependent ferroptosis in V. parahemolyticus. Proteomic analysis revealed that relevant proteins involved in ROS production, lipid peroxidation accumulation, and DNA repair were significantly upregulated after thymol treatment. Molecular docking revealed two potential binding sites (amino acids 46H and 42F) between thymol and ferritin, and thymol could promote the release of Fe2+ from ferritin proteins through in vitro interactions analyzed. Therefore, we hypothesized that ferritin as a potential target may mediate thymol-induced ferroptosis in V. parahemolyticus. This study provides new ideas for the development of natural inhibitors for controlling V. parahemolyticus in aquatic products.


Asunto(s)
Antibacterianos , Ferroptosis , Peróxido de Hidrógeno , Especies Reactivas de Oxígeno , Timol , Vibrio parahaemolyticus , Ferroptosis/efectos de los fármacos , Timol/farmacología , Timol/química , Especies Reactivas de Oxígeno/metabolismo , Vibrio parahaemolyticus/efectos de los fármacos , Peróxido de Hidrógeno/metabolismo , Antibacterianos/farmacología , Antibacterianos/química , Peroxidación de Lípido/efectos de los fármacos , Hierro/metabolismo , Simulación del Acoplamiento Molecular , Ferritinas/genética , Ferritinas/metabolismo , Proteínas Bacterianas/metabolismo , Proteínas Bacterianas/genética
4.
Appl Environ Microbiol ; 90(6): e0053924, 2024 Jun 18.
Artículo en Inglés | MEDLINE | ID: mdl-38809043

RESUMEN

Antibiotics are often used to treat severe Vibrio infections, with third-generation cephalosporins and tetracyclines combined or fluoroquinolones alone being recommended by the US Centers for Disease Control and Prevention. Increases in antibiotic resistance of both environmental and clinical vibrios are of concern; however, limited longitudinal data have been generated among environmental isolates to inform how resistance patterns may be changing over time. Hence, we evaluated long-term trends in antibiotic resistance of vibrios isolated from Chesapeake Bay waters (Maryland) across two 3-year sampling periods (2009-2012 and 2019-2022). Vibrio parahaemolyticus (n = 134) and Vibrio vulnificus (n = 94) toxR-confirmed isolates were randomly selected from both sampling periods and tested for antimicrobial susceptibility against eight antibiotics using the Kirby-Bauer disk diffusion method. A high percentage (94%-96%) of V. parahaemolyticus isolates from both sampling periods were resistant to ampicillin and only 2%-6% of these isolates expressed intermediate resistance or resistance to third-generation cephalosporins, amikacin, tetracycline, and trimethoprim-sulfamethoxazole. Even lower percentages of resistant V. vulnificus isolates were observed and those were mostly recovered from 2009 to 2012, however, the presence of multiple virulence factors was observed. The frequency of multi-drug resistance was relatively low (6%-8%) but included resistance against antibiotics used to treat severe vibriosis in adults and children. All isolates were susceptible to ciprofloxacin, a fluoroquinolone, indicating its sustained efficacy as a first-line agent in the treatment of severe vibriosis. Overall, our data indicate that antibiotic resistance patterns among V. parahaemolyticus and V. vulnificus recovered from the lower Chesapeake Bay have remained relatively stable since 2009.IMPORTANCEVibrio spp. have historically been susceptible to most clinically relevant antibiotics; however, resistance and intermediate-resistance have been increasingly recorded in both environmental and clinical isolates. Our data showed that while the percentage of multi-drug resistance and resistance to antibiotics was relatively low and stable across time, some Vibrio isolates displayed resistance and intermediate resistance to antibiotics typically used to treat severe vibriosis (e.g., third-generation cephalosporins, tetracyclines, sulfamethoxazole-trimethoprim, and aminoglycosides). Also, given the high case fatality rates observed with Vibrio vulnificus infections, the presence of multiple virulence factors in the tested isolates is concerning. Nevertheless, the continued susceptibility of all tested isolates against ciprofloxacin, a fluoroquinolone, is indicative of its use as an effective first-line treatment of severe Vibrio spp. infections stemming from exposure to Chesapeake Bay waters or contaminated seafood ingestion.


Asunto(s)
Antibacterianos , Bahías , Vibrio parahaemolyticus , Vibrio vulnificus , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/aislamiento & purificación , Vibrio vulnificus/efectos de los fármacos , Vibrio vulnificus/aislamiento & purificación , Vibrio vulnificus/crecimiento & desarrollo , Bahías/microbiología , Antibacterianos/farmacología , Estudios Longitudinales , Maryland , Pruebas de Sensibilidad Microbiana , Farmacorresistencia Bacteriana , Vibriosis/microbiología , Humanos
5.
Aquat Toxicol ; 272: 106959, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38768528

RESUMEN

As one of the main components of marine pollution, microplastics (MPs) inevitably enter the mussel aquaculture environment. At the same time, pathogenic bacteria, especially pathogens such as Vibrio, can cause illness outbreaks, leading to large-scale death of mussels. The potential harm of MPs and pathogenic bacteria to bivalve remains unclear. This study designed two experiments (1) mussels (Mytilus galloprovincialis) were exposed to 100 particles/L or 1,000 particles/L polymethyl methacrylate (PMMA, 17.01 ± 6.74 µm) MPs and 1 × 107 CFU/mL Vibrio parahaemolyticus at the same time (14 days), and (2) mussels were exposed to 100 particles/L or 1,000 particles/L MPs for a long time (30 days) and then exposed to 1 × 107 CFU/mL V. parahaemolyticus to explore the effects of these two stresses on the mussel immune system. The results showed that after the combined exposure of V. parahaemolyticus and MPs, the lysosomal membrane stability of hemocytes decreased, lysozyme activity was inhibited, and hemocytes were induced to produce more lectins and defensins to fight pathogenic invasion. Long-term exposure to MPs caused a large amount of energy consumption in mussels, inhibited most of the functions of humoral immunity, increased the risk of mussel infection with pathogenic bacteria, and negatively affected mussel condition factor, the number of hemocytes, and the number of byssuses. Mussels may allocate more energy to deal with MPs and pathogenic bacterial infections rather than for growth. Above all, MPs exposure can affect mussel immune function or reduce its stress resistance, which in turn has an impact on mollusk farming.


Asunto(s)
Hemocitos , Microplásticos , Mytilus , Vibrio parahaemolyticus , Contaminantes Químicos del Agua , Animales , Mytilus/microbiología , Mytilus/efectos de los fármacos , Mytilus/inmunología , Microplásticos/toxicidad , Vibrio parahaemolyticus/fisiología , Vibrio parahaemolyticus/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Hemocitos/efectos de los fármacos , Hemocitos/inmunología , Muramidasa/metabolismo , Sistema Inmunológico/efectos de los fármacos
6.
J Invertebr Pathol ; 205: 108142, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38788921

RESUMEN

This study aims to investigate the use of pond apple (Annona glabra) compounds as a novel strategy to prevent and treat acute hepatopancreatic necrosis disease (AHPND) as well as to better understand the mechanism of health improvement in shrimp. The A. glabra leaf extracts were extracted using various solvents and examined for in vitro and in vivo activity against Vibrio parahaemolyticus strains. In comparison with ethanol and water extracts, methanol extract showed the strongest bactericidal effect (MBC/MIC ratio of 2.50 ± 1.00), with minimal inhibitory concentration (MIC) of 0.023 ± 0.012 mg ml-1 and minimum bactericidal concentration (MBC) of 0.065 ± 0.062 mg ml-1. White leg shrimp (P. vannamei, body weight 10.37 ± 0.27 g) fed A. glabra methanol extracts-containing diets (AMEDs) at 1 %, 1.5 %, and 2.0 % demonstrated no deleterious effects on survival and were significantly increased in length and weight after 30 days of feeding. The level of total haemocyte, hyaline haemocyte on day 15 and granulocyte on day 30 remarkably increased (p < 0.05) in shrimps fed AMEDs groups compared to those in the control group. The finding demonstrates that granulocyte was induced time dependently. In particular, the survival rate of V. parahaemolyticus challenged shrimps under medication with AMEDs at 1.5 % and 2.0 % was significantly higher (p < 0.05) than that of the control group. The decrease in bacterial load of Vibrio spp. and V. parahaemolyticus was obviously recorded in hepatopancreas shrimp given AMEDs 1.5 % and 2.0 % and may be linked to herb characteristics such as antibacterial activity, enhancing innate immunity, and its potential to maintain the integrity of hepatopancreatic tissue. Our findings suggest that A. glabra extract might be used as a health enhancer in commercial farmed shrimp.


Asunto(s)
Annona , Hepatopáncreas , Penaeidae , Extractos Vegetales , Vibrio parahaemolyticus , Animales , Penaeidae/microbiología , Penaeidae/efectos de los fármacos , Extractos Vegetales/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Annona/química , Hepatopáncreas/efectos de los fármacos , Hepatopáncreas/patología , Antibacterianos/farmacología
7.
Sci Rep ; 14(1): 11584, 2024 05 21.
Artículo en Inglés | MEDLINE | ID: mdl-38773245

RESUMEN

Climate change and disease threaten shrimp farming. Here, we studied the beneficial properties of a phytogenic formulation, Shrimp Best (SB), in whiteleg shrimp. Functional studies showed that SB dose-dependently increased shrimp body weight and decreased feed conversion ratio. We found that SB protected against Vibrio parahaemolyticus as evidenced by survival rate, bacterial load, and hepatopancreatic pathology in shrimp. Finally, we explored the likely mechanism by which SB affects growth performance and vibriosis in shrimp. The 16S rRNA sequencing data showed that SB increased 6 probiotic genera and decreased 6 genera of pathogenic bacteria in shrimp. Among these, SB increased the proportion of Lactobacillus johnsonii and decreased that of V. parahaemolyticus in shrimp guts. To dissect the relationship among SB, Lactobacillus and Vibrio, we investigated the in vitro regulation of Lactobacillus and Vibrio by SB. SB at ≥ 0.25 µg/mL promoted L. johnsonii growth. Additionally, L. johnsonii and its supernatant could inhibit V. parahaemolyticus. Furthermore, SB could up-regulate five anti-Vibrio metabolites of L. johnsonii, which caused bacterial membrane destruction. In parallel, we identified 3 fatty acids as active compounds from SB. Overall, this work demonstrated that SB improved growth performance and vibriosis protection in shrimp via the regulation of gut microbiota.


Asunto(s)
Penaeidae , Vibrio parahaemolyticus , Animales , Penaeidae/microbiología , Penaeidae/crecimiento & desarrollo , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/crecimiento & desarrollo , Vibrio parahaemolyticus/patogenicidad , Vibriosis/prevención & control , Vibriosis/veterinaria , Vibriosis/microbiología , Lactobacillus/crecimiento & desarrollo , ARN Ribosómico 16S/genética , Vibrio/efectos de los fármacos , Vibrio/patogenicidad , Probióticos
8.
Fish Shellfish Immunol ; 149: 109615, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38719095

RESUMEN

Curcumin (Cur) exhibits diverse natural pharmacological activities, despite its limited water solubility (hydrophobicity) and low bioavailability. In this investigation, a valine-curcumin conjugate (Val-Cur) was synthesized through amino acid side chain modification, and its solubility increased to 1.78 mg/mL. In vitro experimental findings demonstrated that the antibacterial activity of Val-Cur against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, and Vibrio parahaemolyticus was significantly superior to that of Cur. The inhibition rate of Val-Cur against HepG2 (human hepatocellular carcinoma) cells was higher than that of Cur at low concentrations (below 25 µmol/L), although the IC50 value of Val-Cur did not differ significantly from that of Cur. In vivo biological effects of Val-Cur were assessed by adding it into the feed (150 mg/kg) of American eels (Anguilla rostrata). Val-Cur significantly improved the growth performance (↑weight gain rate, ↑specific growth rate, and ↓feed conversion rate) and activities of intestinal digestive enzymes (amylase and lipase) and antioxidant enzymes (superoxide dismutase) in American eels. Additionally, Val-Cur significantly improved serum biochemical indices (↑high-density lipoprotein cholesterol, ↓low-density lipoprotein cholesterol, ↓aspartate and alanine aminotransferases). Furthermore, Val-Cur increased intestinal microbial diversity, reduced the abundance of potentially pathogenic bacteria (Spiroplasma, Clostridium, and Pseudomonas), and elevated the abundance of beneficial digestion-promoting bacteria (Romboutsia, Phyllobacterium, Romboutsia sedimentorum, and Clostridium butyricum) conducive to glucose metabolism (P < 0.05). To the best of our knowledge, this study is the first to explore water-soluble curcumin in aquaculture, and the findings will lay the groundwork for the potential application of water-soluble curcumin in the field of aquaculture.


Asunto(s)
Anguilla , Antibacterianos , Antineoplásicos , Curcumina , Animales , Curcumina/farmacología , Curcumina/química , Curcumina/administración & dosificación , Antibacterianos/farmacología , Antibacterianos/química , Antineoplásicos/farmacología , Antineoplásicos/química , Valina/farmacología , Valina/química , Alimentación Animal/análisis , Dieta/veterinaria , Humanos , Suplementos Dietéticos/análisis , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/fisiología , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/fisiología , Células Hep G2 , Aeromonas hydrophila/fisiología , Aeromonas hydrophila/efectos de los fármacos
9.
Int J Food Microbiol ; 418: 110737, 2024 Jun 16.
Artículo en Inglés | MEDLINE | ID: mdl-38749264

RESUMEN

Prevalent in marine, estuarine and coastal environments, Vibrio parahaemolyticus is one of the major foodborne pathogens which can cause acute gastroenteritis through consumption of contaminated food. This study encompassed antimicrobial resistance, molecular characteristics and phylogenetic relationships of 163 V. parahaemolyticus isolated from aquatic foods across 15 provinces in China. The isolates showed high resistance rates against ampicillin (90.80 %, 148/163) and cefazolin (72.39 %, 118/163). Only 5 isolates demonstrated multi-drug resistance (MDR) phenotypes. A total of 37 different antibiotic resistance genes (ARGs) in correlation with seven antimicrobial categories were identified. tet(34) and tet(35) were present in all 163 isolates. Other most prevalent ARGs were those conferring resistance to ß-lactams, with prevalence rate around 18.40 % (30/163). The virulence genes tdh and trh were found in 17 (10.43 %) and 9 (5.52 %) isolates, respectively. Totally 121 sequence types (STs) were identified through whole genome analysis, among which 60 were novel. The most prevalent sequence type was ST3 (9.20 %, 15/163), which shared the same genotype profile of trh_, tdh+ and blaCARB-22+. Most of the tdh+V. parahaemolyticus isolates was clustered into a distinctive clade by the phylogenetic analysis. Our study showed that the antimicrobial resistance of V. parahaemolyticus in aquatic foods in China was moderate. However, the emerging of MDR isolates implicate strengthened monitoring is needed for the better treatment of human V. parahaemolyticus infections. High genetic diversity and virulence potential of the isolates analyzed in this study help better understanding and evaluating the risk of V. parahaemolyticus posed to public health.


Asunto(s)
Antibacterianos , Pruebas de Sensibilidad Microbiana , Filogenia , Vibrio parahaemolyticus , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/aislamiento & purificación , Vibrio parahaemolyticus/patogenicidad , Vibrio parahaemolyticus/clasificación , China/epidemiología , Antibacterianos/farmacología , Microbiología de Alimentos , Alimentos Marinos/microbiología , Farmacorresistencia Bacteriana Múltiple/genética , Genoma Bacteriano , Factores de Virulencia/genética , Humanos , Genotipo
10.
Sci Rep ; 14(1): 9160, 2024 04 22.
Artículo en Inglés | MEDLINE | ID: mdl-38644387

RESUMEN

Food-related illnesses have become a growing public concern due to their considerable socioeconomic and medical impacts. Vibrio parahaemolyticus and Staphylococcus aureus have been implicated as causative organisms of food-related infections and poisoning, and both can form biofilms which confer antibiotic resistance. Hence, the need for continuous search for compounds with antibiofilm and antivirulence properties. In this study, 22 iodinated hydrocarbons were screened for their antibiofilm activity, and of these, iodopropynyl butylcarbamate (IPBC) was found to effectively control biofilm formation of both pathogens with a MIC of 50 µg/mL which was bactericidal to V. parahaemolyticus and S. aureus. Microscopic studies confirmed IPBC inhibits biofilm formation of both bacteria and also disrupted their mixed biofilm formation. Furthermore, IPBC suppressed virulence activities such as motility and hemolytic activity of V. parahaemolyticus and the cell surface hydrophobicity of S. aureus. It exhibited a preservative potential against both pathogens in a shrimp model. IPBC disrupted the cell membrane of S. aureus and V. parahaemolyticus and differentially affected gene expressions related to biofilm formation and virulence. Additionally, it displayed broad-spectrum antibiofilm activities against other clinically relevant pathogens. These findings indicate IPBC offers a potential means of controlling infections mediated by Vibrio and Staphylococcus biofilms.


Asunto(s)
Antibacterianos , Biopelículas , Pruebas de Sensibilidad Microbiana , Staphylococcus aureus , Vibrio parahaemolyticus , Biopelículas/efectos de los fármacos , Vibrio parahaemolyticus/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/fisiología , Antibacterianos/farmacología , Antibacterianos/química , Animales , Virulencia/efectos de los fármacos
11.
BMC Microbiol ; 24(1): 145, 2024 Apr 26.
Artículo en Inglés | MEDLINE | ID: mdl-38671363

RESUMEN

BACKGROUND: Vibrio parahaemolyticus is the predominant etiological agent of seafood-associated foodborne illnesses on a global scale. It is essential to elucidate the mechanisms by which this pathogen disseminates. Given the existing research predominantly concentrates on localized outbreaks, there is a pressing necessity for a comprehensive investigation to capture strains of V. parahaemolyticus cross borders. RESULTS: This study examined the frequency and genetic attributes of imported V. parahaemolyticus strains among travelers entering Shanghai Port, China, between 2017 and 2019.Through the collection of 21 strains from diverse countries and regions, Southeast Asia was pinpointed as a significant source for the emergence of V. parahaemolyticus. Phylogenetic analysis revealed clear delineation between strains originating from human and environmental sources, emphasizing that underlying genome data of foodborne pathogens is essential for environmental monitoring, food safety and early diagnosis of diseases. Furthermore, our study identified the presence of virulence genes (tdh and tlh) and approximately 120 antibiotic resistance-related genes in the majority of isolates, highlighting their crucial involvement in the pathogenesis of V. parahaemolyticus. CONCLUSIONS: This research enhanced our comprehension of the worldwide transmission of V. parahaemolyticus and its antimicrobial resistance patterns. The findings have important implications for public health interventions and antimicrobial stewardship strategies, underscoring the necessity for epidemiological surveillance of pathogen at international travel hubs.


Asunto(s)
Enfermedades Transmitidas por los Alimentos , Filogenia , Vibriosis , Vibrio parahaemolyticus , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/aislamiento & purificación , Vibrio parahaemolyticus/clasificación , Vibrio parahaemolyticus/patogenicidad , Vibrio parahaemolyticus/efectos de los fármacos , Humanos , China/epidemiología , Vibriosis/microbiología , Vibriosis/epidemiología , Enfermedades Transmitidas por los Alimentos/microbiología , Enfermedades Transmitidas por los Alimentos/epidemiología , Genoma Bacteriano/genética , Viaje , Factores de Virulencia/genética , Genómica , Farmacorresistencia Bacteriana/genética , Antibacterianos/farmacología , Alimentos Marinos/microbiología
12.
J Fish Dis ; 47(6): e13924, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38300462

RESUMEN

Vibrio harveyi and Vibrio parahaemolyticus are species of the Vibrio genus that often cause disease and mass mortality in crustaceans. If not handled quickly and appropriately, these diseases can cause considerable losses to farmers. Therefore, it is necessary to find a solution with safe and environmentally friendly disease prevention technology using natural ingredients, among others from plants, namely oil palm. Some parts of oil palm, namely leaves, fronds, fibres and oil palm pulp, which are palm waste, contain antibacterial compounds. This study aimed to assess the antibacterial activity of palm waste extracts, namely pulp, leaves, fronds and fibres using n-hexane, ethyl acetate, chloroform, ethanol and water maceration solvents against pathogenic bacteria V. harveyi and V. parahaemolyticus, and identify active compounds contained in palm waste. The results of the research are expected to produce innovative and sustainable solutions to control diseases in shrimp farming, contribute to the development of a sustainable fishing industry and open up the potential for utilizing palm waste as a value-added resource in the field of aquatic health. The results of observations on antibacterial activity tests and identifying the content of palm waste extract compounds were analysed descriptively displayed in the form of figures, tables and graphs. The results showed that palm waste extracts (pulp, leaves, fronds and fibres) with ethyl acetate and ethanol maceration solvents had very strong antibacterial potential, namely 20.14 ± 0.31 mm-25.52 ± 1.42 mm on V. harveyi bacteria and 20.41 ± 0.55 mm-25.00 ± 0.51 mm on V. parahaemolyticus bacteria. Palm extracts with n-hexane (>20 mm) and chloroform solvents generally have strong category antibacterial potential (10-20 mm), and palm extracts in water solvents have medium category potential (5-10 mm) against V. harveyi and V. parahemolyticus bacteria. The results of phytochemical tests on palm waste extracts with ethyl acetate and ethanol maceration solvents contain bioactive compounds of flavonoids, saponins, polyphenols and alkaloid tannins, steroids and triterpenoids. Palm extracts with n-hexane and chloroform solvents generally contain saponins, alkaloids, steroids and triterpenoids, while palm waste extracts with water solvents contain saponins.


Asunto(s)
Antibacterianos , Extractos Vegetales , Vibrio parahaemolyticus , Vibrio , Antibacterianos/farmacología , Antibacterianos/química , Vibrio/efectos de los fármacos , Vibrio parahaemolyticus/efectos de los fármacos , Extractos Vegetales/farmacología , Extractos Vegetales/química , Arecaceae/química , Fitoquímicos/farmacología , Fitoquímicos/química , Fitoquímicos/análisis
14.
J Fish Dis ; 46(5): 477-486, 2023 May.
Artículo en Inglés | MEDLINE | ID: mdl-36656658

RESUMEN

Vibrios belonging to the Harveyi clade (including closely related species such as Vibrio campbellii, Vibrio harveyi and Vibrio parahaemolyticus) are important pathogens of aquatic organisms. In this study, we investigated the use of indole-3-acetic acid to control disease caused by Harveyi clade vibrios. Indole-3-acetic acid, which can be produced by various seaweeds and microalgae, was added to the rearing water of brine shrimp larvae challenged with 12 different Harveyi clade Vibrio strains. Indole-3-acetic acid significantly decreased the virulence of 10 of the strains without any effect on their growth. The latter is important as it will minimize the selective pressure for resistance development. The survival rate of brine shrimp larvae increased from 1.2-fold to 4.8-fold upon treatment with 400 µM indole-3-acetic acid. Additionally, indole-3-acetic acid significantly decreased the swimming motility in 10 of the strains and biofilm formation in eight of the strains. The mRNA levels of the pirA and pirB toxin genes were decreased to 46% and 42% by indole-3-acetic acid in the AHPND-causing strain V. parahaemolyticus M0904. Hence, our data demonstrate that indole-3-acetic acid has the potential to be an effective virulence inhibitor to control infections in aquaculture.


Asunto(s)
Artemia , Ácidos Indolacéticos , Vibrio parahaemolyticus , Animales , Artemia/microbiología , Ácidos Indolacéticos/farmacología , Larva , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/fisiología
15.
Int J Mol Sci ; 22(23)2021 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-34884815

RESUMEN

BACKGROUND: New strategies are needed to combat multidrug-resistant bacteria. The restriction of iron uptake by bacteria is a promising way to inhibit their growth. We aimed to suppress the growth of Vibrio bacterial species by inhibiting their ferric ion-binding protein (FbpA) using food components. METHODS: Twenty spices were selected for the screening of FbpA inhibitors. The candidate was applied to antibacterial tests, and the mechanism was further studied. RESULTS: An active compound, rosmarinic acid (RA), was screened out. RA binds competitively and more tightly than Fe3+ to VmFbpA, the FbpA from V. metschnikovii, with apparent KD values of 8 µM vs. 17 µM. Moreover, RA can inhibit the growth of V. metschnikovii to one-third of the control at 1000 µM. Interestingly, sodium citrate (SC) enhances the growth inhibition effect of RA, although SC only does not inhibit the growth. The combination of RA/SC completely inhibits the growth of not only V. metschnikovii at 100/100 µM but also the vibriosis-causative pathogens V. vulnificus and V. parahaemolyticus, at 100/100 and 1000/100 µM, respectively. However, RA/SC does not affect the growth of Escherichia coli. CONCLUSIONS: RA/SC is a potential bacteriostatic agent against Vibrio species while causing little damage to indigenous gastrointestinal bacteria.


Asunto(s)
Cinamatos/farmacología , Depsidos/farmacología , Hierro/metabolismo , Citrato de Sodio/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Sitios de Unión , Cinamatos/química , Cinamatos/metabolismo , Depsidos/química , Depsidos/metabolismo , Sinergismo Farmacológico , Proteínas de Unión a Hierro/química , Proteínas de Unión a Hierro/metabolismo , Simulación del Acoplamiento Molecular , Extractos Vegetales/química , Unión Proteica , Vibrio parahaemolyticus/metabolismo , Ácido Rosmarínico
16.
J Nanobiotechnology ; 19(1): 448, 2021 Dec 24.
Artículo en Inglés | MEDLINE | ID: mdl-34952588

RESUMEN

BACKGROUND: Shrimp aquaculture has suffered huge economic losses over the past decade due to the outbreak of acute hepatopancreatic necrosis disease (AHPND), which is mainly caused by the bacteria Vibrio parahaemolyticus (V. parahaemolyticus) with the virulence pVA1 plasmid, which encodes a secretory photorhabdus insect-related (Pir) toxin composed of PirA and PirB proteins. The Pir toxin mainly attacks the hepatopancreas, a major metabolic organ in shrimp, thereby causing necrosis and loss of function. The pandemic of antibiotic-resistant strains makes the impact worse. METHODS: Mild pyrolysis of a mixture of polysaccharide dextran 70 and the crosslinker 1,8-diaminooctane at 180 â„ƒ for 3 h to form carbonized nanogels (DAO/DEX-CNGs) through controlled cross-linking and carbonization. The multifunctional therapeutic CNGs inherit nanogel-like structures and functional groups from their precursor molecules. RESULTS: DAO/DEX-CNGs manifest broad-spectrum antibacterial activity against Vibrio parahaemolyticus responsible for AHPND and even multiple drug-resistant strains. The polymer-like structures and functional groups on graphitic-carbon within the CNGs exhibit multiple treatment effects, including disruption of bacterial membranes, elevating bacterial oxidative stress, and neutralization of PirAB toxins. The inhibition of Vibrio in the midgut of infected shrimp, protection of hepatopancreas tissue from Pir toxin, and suppressing overstimulation of the immune system in severe V. parahaemolyticus infection, revealing that CNGs can effectively guard shrimp from Vibrio invasion. Moreover, shrimps fed with DAO/DEX-CNGs were carefully examined, such as the expression of the immune-related genes, hepatopancreas biopsy, and intestinal microbiota. Few adverse effects on shrimps were observed. CONCLUSION: Our work proposes brand-new applications of multifunctional carbon-based nanomaterials as efficient anti-Vibrio agents in the aquatic industry that hold great potential as feed additives to reduce antibiotic overuse in aquaculture.


Asunto(s)
Antiinfecciosos/uso terapéutico , Nanogeles/uso terapéutico , Vibriosis/tratamiento farmacológico , Animales , Antiinfecciosos/química , Antiinfecciosos/metabolismo , Antiinfecciosos/farmacología , Artemia/microbiología , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Materiales Biocompatibles/uso terapéutico , Carbono/química , Dextranos/química , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Hepatopáncreas/patología , Nanogeles/química , Nanogeles/toxicidad , Toxinas Biológicas/química , Toxinas Biológicas/metabolismo , Vibriosis/prevención & control , Vibriosis/veterinaria , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/patogenicidad
17.
J Nat Prod ; 84(12): 3011-3019, 2021 12 24.
Artículo en Inglés | MEDLINE | ID: mdl-34842422

RESUMEN

Six new nonadride derivatives, named talarodrides A-F (1-6), were isolated from the Antarctic sponge-derived fungus Talaromyces sp. HDN1820200. All structures including the absolute configurations were deduced by extensive spectroscopic analysis and computational ECD calculations. Compounds 1-4 share a rare caged bicyclo[4.3.1]-deca-1,6-diene with a bridgehead olefin and maleic anhydride core skeleton, while compounds 5 and 6 possess the first case of a naturally occurring 5/7/6 methanocyclonona[c]furan skeleton. Talarodride A (1) and talarodride B (2) showed selective inhibitory effects against Proteus mirabilis and Vibrio parahemolyticus with MICs of 3.13-12.5 µM.


Asunto(s)
Anhídridos/aislamiento & purificación , Poríferos/microbiología , Talaromyces/química , Anhídridos/química , Anhídridos/farmacología , Animales , Regiones Antárticas , Pruebas de Sensibilidad Microbiana , Proteus mirabilis/efectos de los fármacos , Vibrio parahaemolyticus/efectos de los fármacos
18.
Microb Pathog ; 161(Pt A): 105260, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34688850

RESUMEN

Vibrio parahaemolyticus is responsible for infection diseases of people who consume the contaminated seafood, but its metabolic regulation profile in response to colistin, the last treatment option for multidrug-resistant Gram-negative bacteria, remains unclear. In this study, the metabolic regulation profile of V. parahaemolyticus ATCC33846 under polymyxin B stimulation has been investigated. V. parahaemolyticus exposed to polymyxin B resulted in 4597 differentially transcribed genes, including 673 significantly up-regulated genes and 569 significantly down-regulated genes. In V. parahaemolyticus under polymyxin B stimulation, the cellular antioxidant systems to prevent bacteria from oxidant stress was activated, the synthesis of some nonessential macromolecules was reduced, and the assembly and modification of lipopolysaccharide and peptidoglycan to resist the attack from other antibiotics were promoted. These findings provide new insights into polymyxin B-related stress response in V. parahaemolyticus which should be useful for developing novel drugs for infection.


Asunto(s)
Antibacterianos , Polimixina B , Vibrio parahaemolyticus , Antibacterianos/farmacología , Farmacorresistencia Bacteriana Múltiple , Polimixina B/farmacología , Vibrio parahaemolyticus/efectos de los fármacos , Vibrio parahaemolyticus/genética
19.
Int J Biol Macromol ; 191: 71-78, 2021 Nov 30.
Artículo en Inglés | MEDLINE | ID: mdl-34534580

RESUMEN

In this study, cellulose nanocrystals (CNC) as functional cross-linker and Pickering emulsifier was used to stabilize Lysozyme (Lys) encapsulated in quaternary ammonium chitosan nanoparticles (QC NPs) via ionic gelation method. Physicochemical, structural, and antibacterial properties of the CNC stabilized Lys loaded QC NPs were also evaluated. Particle size, particle size distribution, Zeta potential (ZP), and spectroscopic analyses showed the successful encapsulation of Lys. Antibacterial activity of NPs against Staphylococcus aureus and Vibrio parahaemolyticus was investigated on the basis of inhibition zone (IZ), minimum inhibitory concentration (MIC), and minimum bacterial concentration (MBC). MIC and MBC of CNC stabilized Lys loaded HQC NPs against S. aureus were 0.094 and 0.188 while corresponding values for CNC stabilized Lys loaded LQC NPs V. parahaemolyticus were 0.156 and 0.312 mg/mL, respectively. Therefore, CNC stabilized Lys loaded QC NPs have potential implications in the food industry for food preservation and packaging.


Asunto(s)
Antibacterianos/administración & dosificación , Celulosa/análogos & derivados , Muramidasa/administración & dosificación , Nanopartículas/química , Compuestos de Amonio Cuaternario/química , Antibacterianos/farmacología , Muramidasa/farmacología , Staphylococcus aureus/efectos de los fármacos , Vibrio parahaemolyticus/efectos de los fármacos
20.
World J Microbiol Biotechnol ; 37(8): 145, 2021 Aug 05.
Artículo en Inglés | MEDLINE | ID: mdl-34351514

RESUMEN

Vibrio parahaemolyticus is one of the leading causes of diarrhoea and gastroenteritis in human on consumption of raw or insufficiently cooked seafood. This study was aimed at isolating and characterizing the pathogenic and pandemic V. parahaemolyticus from oysters (n = 90) in coastal parts of West Bengal, India; their antibiotic resistance and potential for involvement in the food chain. During bacteriological culture, typical V. parahaemolyticus colony was recovered in 88.9% samples followed by presumptive identification in 71 (78.9%) samples by characteristic biochemical (K/A) test. All the presumptive isolates (n = 71) were confirmed by species specific Vp-toxR PCR assay. Of these, 10 (14.08%) were tdh+ and none for the trh. Further, 5 (50%) of these tdh+ isolates were found to carry the pandemic potential gene in PGS-PCR assay; however, none in GS-PCR. Majority (80%) of these pathogenic (tdh+) isolates belonged to pandemic serovars (OUT: KUT; OUT: K24; O1: KUT; O1:K25; O10: KUT) and only 20% to non-pandemic serovars (OUT: K15; O9:K17). All the isolates (100%) exhibited resistance to cefpodoxime followed by ampicillin and cefotaxime (90%), ceftizoxime (60%), tetracycline (50%), ceftriaxone (40%), ciprofloxacin and nalidixic acid (10% each). Overall, the study findings suggested that 11.1% (10/90) of commonly marketed oysters in this area were harbouring pathogenic V. parahaemolyticus. Moreover, 5.5% (5/90) of the oyster population were harbouring pandemic strains of this pathogen. Besides, the pathogenic isolates from oysters were exhibiting a considerable genetic relatedness (53 to 70%) to human clinical isolates in PFGE analysis that relates to a substantial public health risk. Further, their multidrug resistance added gravity to the antimicrobial resistance (AMR), a globally growing public health threat and this is a critical area of concern especially during the treatment of foodborne gastroenteritis.


Asunto(s)
Contaminación de Alimentos/análisis , Enfermedades Transmitidas por los Alimentos/microbiología , Ostreidae/microbiología , Mariscos/microbiología , Vibriosis/microbiología , Vibrio parahaemolyticus/efectos de los fármacos , Animales , Antibacterianos/farmacología , Farmacorresistencia Bacteriana , Cadena Alimentaria , Humanos , India , Reacción en Cadena de la Polimerasa , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/crecimiento & desarrollo , Vibrio parahaemolyticus/aislamiento & purificación
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...