RESUMEN
An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent.
Asunto(s)
Virus Fúngicos/genética , Fusarium/virología , Virus Fúngicos/clasificación , Virus Fúngicos/ultraestructura , Fusarium/aislamiento & purificación , Genoma Viral/genética , Pakistán , Filogenia , Enfermedades de las Plantas/microbiología , Plantas/microbiología , Virus ARN/clasificación , Virus ARN/genética , Virus ARN/ultraestructura , ARN Viral/genética , Proteínas Virales/genética , Viroma/genéticaRESUMEN
The family Marnaviridae comprises small non-enveloped viruses with positive-sense RNA genomes of 8.6-9.6 kb. Isolates infect marine single-celled eukaryotes (protists) that come from diverse lineages. Some members are known from metagenomic studies of ocean virioplankton, with additional unclassified viruses described from metagenomic datasets derived from marine and freshwater environments. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Marnaviridae, which is available at ictv.global/report/marnaviridae.
Asunto(s)
Genoma Viral , Filogenia , Virus ARN/clasificación , Virus ARN/genética , Animales , Proteínas de la Cápside , Eucariontes , Especificidad del Huésped , Hidrobiología , Metagenómica , Infecciones por Virus ARN/virología , Virus ARN/ultraestructura , ARN Viral , Virión/clasificación , Virión/genética , Virión/ultraestructura , Replicación ViralRESUMEN
Drosophila suzukii (Ds) is an invasive pest insect that infests ripening fruit, causing severe economic losses. Control measures based on chemical pesticides are inefficient and undesirable, so biological alternatives have been considered, including native Ds viruses. We previously isolated a strain of La Jolla virus (LJV-Ds-OS20) from Ds in Germany as a candidate biopesticide. Here we characterized the new strain in detail, focusing on the processing of its capsid proteins. We tested LJV growth during Ds development to optimize virus production, and established a laboratory production system using adult flies. This system was suitable for the preparation of virions for detailed analysis. The LJV-Ds-OS20 isolate was cloned by limiting dilution and the complete nucleotide sequence was determined as a basis for protein analysis. The terminal segments of the virus genome were completed by RACE-PCR. LJV virions were also purified by CsCl gradient centrifugation and analyzed by SDS-PAGE and electron microscopy. The capsid proteins of purified LJV virions were resolved by two-dimensional SDS-PAGE for N-terminal sequencing and peptide mass fingerprinting. The N-terminal sequences of VP1 and VP2, together with MS data representing several capsid proteins, allowed us to develop a model for the organization of the LJV structural protein region. This may facilitate the development of new viral strains as biopesticides.
Asunto(s)
Drosophila/virología , Especies Introducidas , Virus ARN/genética , Proteínas Estructurales Virales/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Femenino , Secuenciación de Nucleótidos de Alto Rendimiento , Filogenia , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , Virus ARN/ultraestructura , ARN Viral , Análisis de Secuencia de ARN , Carga Viral , Proteínas Estructurales Virales/químicaRESUMEN
Trichomonas vaginalis, the causative pathogen for the most common nonviral sexually transmitted infection worldwide, is itself frequently infected with one or more of the four types of small double-stranded RNA (dsRNA) Trichomonas vaginalis viruses (TVV1 to 4, genus Trichomonasvirus, family Totiviridae). Each TVV encloses a nonsegmented genome within a single-layered capsid and replicates entirely intracellularly, like many dsRNA viruses, and unlike those in the Reoviridae family. Here, we have determined the structure of TVV2 by cryo-electron microscopy (cryoEM) at 3.6 Å resolution and derived an atomic model of its capsid. TVV2 has an icosahedral, T = 2*, capsid comprised of 60 copies of the icosahedral asymmetric unit (a dimer of the two capsid shell protein [CSP] conformers, CSP-A and CSP-B), typical of icosahedral dsRNA virus capsids. However, unlike the robust CSP-interlocking interactions such as the use of auxiliary "clamping" proteins among Reoviridae, only lateral CSP interactions are observed in TVV2, consistent with an assembly strategy optimized for TVVs' intracellular-only replication cycles within their protozoan host. The atomic model reveals both a mostly negatively charged capsid interior, which is conducive to movement of the loosely packed genome, and channels at the 5-fold vertices, which we suggest as routes of mRNA release during transcription. Structural comparison of TVV2 to the Saccharomyces cerevisiae L-A virus reveals a conserved helix-rich fold within the CSP and putative guanylyltransferase domain along the capsid exterior, suggesting conserved mRNA maintenance strategies among Totiviridae This first atomic structure of a TVV provides a framework to guide future biochemical investigations into the interplay between Trichomonas vaginalis and its viruses.IMPORTANCETrichomonas vaginalis viruses (TVVs) are double-stranded RNA (dsRNA) viruses that cohabitate in Trichomonas vaginalis, the causative pathogen of trichomoniasis, the most common nonviral sexually transmitted disease worldwide. Featuring an unsegmented dsRNA genome encoding a single capsid shell protein (CSP), TVVs contrast with multisegmented dsRNA viruses, such as the diarrhea-causing rotavirus, whose larger genome is split into 10 dsRNA segments encoding 5 unique capsid proteins. To determine how TVVs incorporate the requisite functionalities for viral replication into their limited proteome, we derived the atomic model of TVV2, a first for TVVs. Our results reveal the intersubunit interactions driving CSP association for capsid assembly and the properties that govern organization and maintenance of the viral genome. Structural comparison between TVV2 capsids and those of distantly related dsRNA viruses indicates conserved strategies of nascent RNA release and a putative viral guanylyltransferase domain implicated in the cytoplasmic maintenance of viral messenger and genomic RNA.
Asunto(s)
Virus ARN/ultraestructura , ARN Bicatenario/química , Totiviridae/ultraestructura , Trichomonas vaginalis/virología , Cápside/química , Cápside/metabolismo , Proteínas de la Cápside/química , Proteínas de la Cápside/genética , Proteínas de la Cápside/metabolismo , Microscopía por Crioelectrón , Genoma Viral , Conformación Proteica en Hélice alfa , Virus ARN/clasificación , Virus ARN/genética , Virus ARN/aislamiento & purificación , ARN Bicatenario/genética , ARN Bicatenario/metabolismo , ARN Viral/química , ARN Viral/genética , ARN Viral/metabolismo , Totiviridae/clasificación , Totiviridae/genética , Totiviridae/aislamiento & purificaciónRESUMEN
Pseudoviridae is a family of reverse-transcribing viruses with long terminal repeats (LTRs) belonging to the order Ortervirales. Pseudoviruses are commonly found integrated in the genomes of diverse plants, fungi and animals and are broadly known as Ty1/Copia LTR retrotransposons. Inside the cell, they form icosahedral virus particles, but unlike most other viruses, do not have an extracellular phase. This is a summary of the ICTV Report on the family Pseudoviridae, which is available at ictv.global/report/pseudoviridae.
Asunto(s)
Virus ARN/clasificación , Virus ARN/genética , Retroelementos , Genoma Viral , Virus ARN/fisiología , Virus ARN/ultraestructura , ARN Viral/genética , Secuencias Repetidas Terminales , Replicación ViralRESUMEN
Ribonucleic acids (RNAs) play essential roles in living cells. Many of them fold into defined three-dimensional (3D) structures to perform functions. Recent advances in single-particle cryo-electron microscopy (cryo-EM) have enabled structure determinations of RNA to atomic resolutions. However, most RNA molecules are structurally flexible, limiting the resolution of their structures solved by cryo-EM. In modeling these molecules, several computational methods are limited by the requirement of massive computational resources and/or the low efficiency in exploring large-scale structural variations. Here we use hierarchical natural move Monte Carlo (HNMMC), which takes advantage of collective motions for groups of nucleic acid residues, to refine RNA structures into their cryo-EM maps, preserving atomic details in the models. After validating the method on a simulated density map of tRNA, we applied it to objectively obtain the model of the folding intermediate for the specificity domain of ribonuclease P from Bacillus subtilis and refine a flexible ribosomal RNA (rRNA) expansion segment from the Mycobacterium tuberculosis (Mtb) ribosome in different conformational states. Finally, we used HNMMC to model atomic details and flexibility for two distinct conformations of the complete genomic RNA (gRNA) inside MS2, a single-stranded RNA virus, revealing multiple pathways for its capsid assembly.
Asunto(s)
Método de Montecarlo , Virus ARN/ultraestructura , ARN Ribosómico/ultraestructura , ARN de Transferencia/ultraestructura , ARN/ultraestructura , Ribosomas/ultraestructura , Bacillus subtilis/enzimología , Proteínas de la Cápside/genética , Proteínas de la Cápside/ultraestructura , Modelos Moleculares , ARN/genética , Virus ARN/genética , ARN Ribosómico/genética , ARN de Transferencia/genética , Ribonucleasa P/genética , Ribonucleasa P/ultraestructura , Ribosomas/genéticaRESUMEN
The genomes of RNA viruses contain regulatory elements of varying complexity. Many plus-strand RNA viruses employ largescale intra-genomic RNA-RNA interactions as a means to control viral processes. Here, we describe an elaborate RNA structure formed by multiple distant regions in a tombusvirus genome that activates transcription of a viral subgenomic mRNA. The initial step in assembly of this intramolecular RNA complex involves the folding of a large viral RNA domain, which generates a discontinuous binding pocket. Next, a distally-located protracted stem-loop RNA structure docks, via base-pairing, into the binding site and acts as a linchpin that stabilizes the RNA complex and activates transcription. A multi-step RNA folding pathway is proposed in which rate-limiting steps contribute to a delay in transcription of the capsid protein-encoding viral subgenomic mRNA. This study provides an exceptional example of the complexity of genome-scale viral regulation and offers new insights into the assembly schemes utilized by large intra-genomic RNA structures.
Asunto(s)
Genoma Viral/genética , Conformación de Ácido Nucleico , Virus ARN/ultraestructura , Proteínas Virales/genética , Emparejamiento Base , Virus ARN/genética , ARN Viral/genética , ARN Viral/ultraestructura , Tombusvirus/genética , Tombusvirus/ultraestructura , Transcripción Genética , Proteínas Virales/ultraestructura , Replicación Viral/genéticaRESUMEN
RNA-dependent RNA polymerases (RdRps) catalyze RNA synthesis of RNA viruses. During initiation of RNA synthesis, the RdRp catalyzes the formation of the first dinucleotide, acting as primer for subsequent processive RNA elongation. Here, we present the structure of the RdRp complexes in the dinucleotide primed state in situ within a transcribing cypovirus under near physiological conditions using cryo-electron microscopy. The 3' end of RNA templates, paired RNA dinucleotide primer, incoming nucleotide, and catalytic divalent cations in the RdRp were resolved at 3.8 Å resolution. The end of the RNA template and the dinucleotide is buttressed by the aromatic tyrosine in a loop from the RdRp bracelet domain. Our structure reveals the interactions between the nucleotide substrates and the conserved residues during the RdRp initiation, and the coordinated structural changes preceding the elongation stage. In addition, it provides the direct evidence for existence of the slow step of the dinucleotide primed state in the viral RdRp transcription.
Asunto(s)
Virus ARN/ultraestructura , ARN Polimerasa Dependiente del ARN/ultraestructura , ARN/biosíntesis , Reoviridae/ultraestructura , Microscopía por Crioelectrón , Complejos Multiproteicos , Conformación Proteica , ARN/química , ARN/genética , Virus ARN/enzimología , ARN Polimerasa Dependiente del ARN/química , ARN Polimerasa Dependiente del ARN/genética , Reoviridae/química , Reoviridae/genética , Transcripción GenéticaRESUMEN
This report describes and characterizes three novel RNA viruses isolated from dead birds collected during West Nile virus surveillance in Harris County, TX, USA (the Houston metropolitan area). The novel viruses are identified as members of the families Nyamaviridae, Orthomyxoviridae, and Peribunyaviridae and have been designated as San Jacinto virus, Mason Creek virus, and Buffalo Bayou virus, respectively. Their potential public health and/or veterinary importance are still unknown.
Asunto(s)
Aves/virología , Orthomyxoviridae , Virus ARN , Animales , Enfermedades de las Aves/virología , Ratones , Orthomyxoviridae/clasificación , Orthomyxoviridae/genética , Orthomyxoviridae/aislamiento & purificación , Filogenia , Virus ARN/clasificación , Virus ARN/genética , Virus ARN/aislamiento & purificación , Virus ARN/ultraestructura , ARN Viral , TexasRESUMEN
Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.
Asunto(s)
Microscopía por Crioelectrón/métodos , Virus ADN/ultraestructura , Virus ARN/ultraestructura , Virión/ultraestructuraRESUMEN
Members of the family Mymonaviridae produce filamentous, enveloped virions containing a single molecule of linear, negative-sense RNA of ≈10 kb. The family currently includes a single genus, Sclerotimonavirus. Mymonaviruses usually infect filamentous fungi, and one virus, Sclerotinia sclerotiorum negative-stranded RNA virus 1, induces hypovirulence in the fungal host. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Mymonaviridae, which is available at ictv.global/report/mymonaviridae.
Asunto(s)
Virus Fúngicos/clasificación , Hongos/virología , Virus ARN/clasificación , Virus Fúngicos/genética , Virus Fúngicos/aislamiento & purificación , Virus Fúngicos/ultraestructura , Genoma Viral , Filogenia , Virus ARN/genética , Virus ARN/aislamiento & purificación , Virus ARN/ultraestructura , Virión/clasificación , Virión/genética , Virión/aislamiento & purificación , Virión/ultraestructuraRESUMEN
The family Artoviridae was created in 2018 for the established monospecific genus Peropuvirus and six new species of invertebrate viruses that had all been discovered by high-throughput sequencing. Artoviruses have negative-sense RNA genomes of about 12 kb and produce enveloped, spherical particles that are 100-130 nm in diameter. Hosts include parasitoid wasps, barnacles, pillworms, woodlice, copepods and odonates. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Artoviridae, which is available at www.ictv.global/report/artoviridae.
Asunto(s)
Virus ARN/clasificación , Animales , Genoma Viral , Secuenciación de Nucleótidos de Alto Rendimiento , Especificidad del Huésped , Invertebrados/virología , Virus ARN/genética , Virus ARN/fisiología , Virus ARN/ultraestructura , Avispas/virologíaRESUMEN
Polycipiviridae is a family of picorna-like viruses with non-segmented, linear, positive-sense RNA genomes of approximately 10-12 kb. Unusually for viruses within the order Picornavirales, their genomes are polycistronic, with four (or more) consecutive 5'-proximal open reading frames (ORFs) encoding structural (and possibly other) proteins and a long 3' ORF encoding the replication polyprotein. Members of species within the family have all been detected in ants or via arthropod transcriptomic datasets. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the Polycipiviridae, which is available at www.ictv.global/report/polycipiviridae.
Asunto(s)
Virus ARN/clasificación , Animales , Hormigas/virología , Genoma Viral , Sistemas de Lectura Abierta , Filogenia , Virus ARN/genética , Virus ARN/aislamiento & purificación , Virus ARN/ultraestructura , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Double-stranded (ds) RNA is produced as a replicative intermediate during RNA virus infection. Recognition of dsRNA by host pattern recognition receptors (PRRs) such as the retinoic acid (RIG-I) like receptors (RLRs) RIG-I and melanoma differentiation-associated protein 5 (MDA-5) leads to the induction of the innate immune response. The formation and intracellular distribution of dsRNA in positive-sense RNA virus infection has been well characterized by microscopy. Many negative-sense RNA viruses, including some arenaviruses, trigger the innate immune response during infection. However, negative-sense RNA viruses were thought to produce low levels of dsRNA, which hinders the imaging study of PRR recognition of viral dsRNA. Additionally, infection experiments with highly pathogenic arenaviruses must be performed in high containment biosafety level facilities (BSL-4). The interaction between viral RNA and PRRs for highly pathogenic RNA virus is largely unknown due to the additional technical challenges that researchers need to face in the BSL-4 facilities. Recently, a monoclonal antibody (Mab) (clone 9D5) originally used for pan-enterovirus detection has been found to specifically detect dsRNA with a higher sensitivity than the traditional J2 or K1 anti-dsRNA antibodies. Herein, by utilizing the 9D5 antibody, we describe a confocal microscopy protocol that has been used successfully to visualize dsRNA, viral protein and PRR simultaneously in individual cells infected by arenavirus. The protocol is also suitable for imaging studies of dsRNA and PRR distribution in pathogenic arenavirus infected cells in BSL4 facilities.
Asunto(s)
Inmunidad Innata/inmunología , Microscopía Confocal/métodos , Infecciones por Virus ARN/metabolismo , Virus ARN/fisiología , ARN Bicatenario/metabolismo , ARN Viral/metabolismo , Receptores de Reconocimiento de Patrones/metabolismo , Humanos , Infecciones por Virus ARN/inmunología , Infecciones por Virus ARN/virología , Virus ARN/inmunología , Virus ARN/ultraestructura , ARN Bicatenario/ultraestructura , ARN Viral/análisis , Receptores de Reconocimiento de Patrones/análisis , Proteínas Virales/genética , Proteínas Virales/inmunologíaRESUMEN
Mycoviruses ubiquitously infect a wide range of fungal hosts in the world. The current study reports a novel double stranded RNA (dsRNA) virus, termed Alternaria alternata botybirnavirus 1 (AaBbV1), infecting a Pakistani strain, 4a, of a phytopathogenic ascomycetous fungus Alternaria alternata. A combined approach of next generation and conventional terminal end sequencing of the viral genome revealed that the virus is a distinct member of the genus Botybirnavirus. This virus comprised of two segments (dsRNA1 and dsRNA2) of sizes 6127 bp and 5860 bp respectively. The dsRNA1-encoded protein carrying the RNA-dependent RNA polymerase domain showed 61% identity to the counterpart of Botrytis porri botybirnavirus 1 and lower levels of amino acid similarity with those of other putative botybirnaviruses and the fungal dsRNA viruses such as members of the families Totiviridae, Chrysoviridae and Megabirnaviridae. The dsRNA2-encoded protein showed resemblance with corresponding proteins of botybirnaviruses. Electron microscopy showed AaBbV1 to form spherical particles of 40 nm in diameter. Biochemical analyses showed that two structural proteins encoded by dsRNA1 and dsRNA2 underwent processing to some extent during particle purification, resulting in the appearance of multiple smaller products. Phylogenetic analyses of structural proteins suggested that their coding region might have been duplicated once and maintained without recombination. Protoplast fusion technique allowed for the introduction of AaBbV1 into a virus free Japanese strain of A. alternata and demonstrated its symptomless infection by the virus. Interesting similarities and dissimilarities between AaBbV1 and other previously reported botybirnaviruses are also discussed.
Asunto(s)
Alternaria/virología , Virus Fúngicos/clasificación , Virus Fúngicos/aislamiento & purificación , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , Análisis por Conglomerados , Virus Fúngicos/genética , Virus Fúngicos/ultraestructura , Genoma Viral , Microscopía Electrónica , Pakistán , Filogenia , Procesamiento Proteico-Postraduccional , Virus ARN/genética , Virus ARN/ultraestructura , ARN Bicatenario/genética , ARN Viral/genética , ARN Polimerasa Dependiente del ARN/genética , Análisis de Secuencia de ADN , Homología de Secuencia , Proteínas Estructurales Virales/metabolismo , Virión/ultraestructuraRESUMEN
The family Sarthroviridae includes a single genus, Macronovirus, which in turn includes a single species, Macrobrachium satellite virus 1. Members of this species, named extra small virus, are satellite viruses of Macrobrachium rosenbergii nodavirus, an unclassified virus related to members of the family Nodaviridae. Both viruses have isometric, spherical virions, infect giant freshwater prawns and together cause white tail disease, which is responsible for mass mortalities and severe economic losses in hatcheries and farms. Infection is caused by both vertical and horizontal transmission of virus. Aquatic insects act as a carrier to transmit the disease in prawns. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Sarthroviridae, which is available at www.ictv.global/report/sarthroviridae.
Asunto(s)
Nodaviridae/crecimiento & desarrollo , Virus ARN/clasificación , Virus ARN/genética , Virus Satélites/clasificación , Virus Satélites/genética , Animales , Transmisión de Enfermedad Infecciosa , Transmisión Vertical de Enfermedad Infecciosa , Insectos Vectores/virología , Nodaviridae/ultraestructura , Palaemonidae/virología , Infecciones por Virus ARN/transmisión , Infecciones por Virus ARN/veterinaria , Infecciones por Virus ARN/virología , Virus ARN/aislamiento & purificación , Virus ARN/ultraestructura , Virus Satélites/aislamiento & purificación , Virus Satélites/ultraestructura , Virión/ultraestructuraRESUMEN
Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). These capsids show structural variations of the same framework, with 60 asymmetric or symmetric homodimers for ScV-L-A and PsV-F, respectively, monomers with a duplicated similar domain for PcV, and heterodimers of two different proteins for RnQV1. Mycovirus capsid proteins (CP) share a conserved α-helical domain, although the latter may carry different peptides inserted at preferential hotspots. Insertions in the CP outer surface are likely associated with enzymatic activities. Within the capsid, fungal dsRNA viruses show a low degree of genome compaction compared to reoviruses, and contain one to two copies of the RNA-polymerase complex per virion.
Asunto(s)
Cápside/ultraestructura , Virus Fúngicos/ultraestructura , Virus ARN/ultraestructura , Proteínas de la Cápside/química , Penicillium chrysogenum/virología , Conformación Proteica , Saccharomyces cerevisiae/virología , Xylariales/virologíaRESUMEN
Here we describe a novel (-)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (-)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.
Asunto(s)
Virus Fúngicos/clasificación , Virus Fúngicos/aislamiento & purificación , Fusarium/virología , Virus ARN/clasificación , Virus ARN/aislamiento & purificación , Virus Fúngicos/genética , Virus Fúngicos/ultraestructura , Genoma Viral , Sistemas de Lectura Abierta , Filogenia , Plantas/microbiología , Virus ARN/genética , Virus ARN/ultraestructura , ARN Viral/genética , Virión/ultraestructuraRESUMEN
The family Ampullaviridae includes viruses with linear dsDNA genomes that replicate in hyperthermophilic archaea from the genus Acidianus. The virions have a unique champagne bottle-shaped morphology and consist of a nucleoprotein filament condensed into a cone-shaped core, which is encased by an envelope, with the base of the 'bottle' decorated with a ring of 20 filaments. Genome replication is presumably carried out by the virus-encoded protein-primed family B DNA polymerase. The bottle-shaped morphology is unprecedented among viruses of bacteria and eukaryotes and represents a group of archaea-specific virion morphotypes. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Ampullaviridae, which is available at www.ictv.global/report/ampullaviridae.
Asunto(s)
Archaea/virología , Bacteriófagos/clasificación , Virus ARN/clasificación , Bacteriófagos/genética , Bacteriófagos/aislamiento & purificación , Bacteriófagos/ultraestructura , Genoma Viral , Filogenia , Virus ARN/genética , Virus ARN/fisiología , Virus ARN/ultraestructura , Virión/clasificación , Virión/genética , Virión/fisiología , Virión/ultraestructura , Replicación ViralRESUMEN
The Chrysoviridae is a family of small, isometric, non-enveloped viruses (40 nm in diameter) with segmented dsRNA genomes (typically four segments). The genome segments are individually encapsidated and together comprise 11.5-12.8 kbp. The single genus Chrysovirus includes nine species. Chrysoviruses lack an extracellular phase to their life cycle; they are transmitted via intracellular routes within an individual during hyphal growth, in asexual or sexual spores, or between individuals via hyphal anastomosis. There are no known natural vectors for chrysoviruses. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Chrysoviridae, which is available at www.ictv.global/report/chrysoviridae.