Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 20
Filtrar
Más filtros












Base de datos
Intervalo de año de publicación
1.
J Cell Physiol ; 236(2): 1295-1308, 2021 02.
Artículo en Inglés | MEDLINE | ID: mdl-32662079

RESUMEN

Adipose-derived stromal cells (ADSCs) showed excellent capacity in regeneration and tissue protection. Low tidal volume ventilation (LVT) strategy demonstrates a therapeutic benefit on the treatment of acute lung injury/acute respiratory distress syndrome (ALI/ARDS). This study, therefore, aimed to undertaken determine whether the combined LVT and ADSCs treatment exerts additional protection against lipopolysaccharide (LPS)-induced ALI in rats. The animals were randomized into seven groups: Group I (control), Group II (instillation of LPS at 10 mg/kg intratracheally), Group III (LPS+LVT 6 ml/kg), Group IV (LPS+intravenous autologous 5 × 106 ADSCs which were pretreated with a scrambled small interfering RNA [siRNA] of keratinocyte growth factor [KGF] negative control), Group V (LPS+ADSCs which were pretreated with a scrambled siRNA of KGF, Group VI (LPS+LVT and ADSCs as in the Group IV), and Group VII (LPS+LVT and ADSCs as in the Group V). We found that levels of tumor necrosis factor-α, transforming growth factor-ß1, and interleukin (IL)-1ß and IL-6, the proinflammatory cytokines, were remarkably increased in LPS rats. Moreover, the expressions of ENaC, activity of Na, K-ATPase, and alveolar fluid clearance (AFC) were obviously reduced by LPS-induced ALI. The rats treated by ADSCs showed improved effects in all these changes of ALI and further enhanced by ADSCs combined with LVT treatment. Importantly, the treatment of ADSCs with siRNA-mediated knockdown of KGF partially eliminated the therapeutic effects. In conclusion, combined treatment with ADSCs and LVT not only is superior to either ADSCs or LVT therapy alone in the prevention of ALI. Evidence of the beneficial effect may be partly due to improving AFC by paracrine or systemic production of KGF and anti-inflammatory properties.


Asunto(s)
Lesión Pulmonar Aguda/terapia , Trasplante de Células Madre Mesenquimatosas , Trasplante Autólogo , Lesión Pulmonar Aguda/inducido químicamente , Lesión Pulmonar Aguda/patología , Tejido Adiposo/citología , Tejido Adiposo/trasplante , Animales , Modelos Animales de Enfermedad , Humanos , Lipopolisacáridos/toxicidad , Pulmón/patología , Células Madre Mesenquimatosas , Ratas , Respiración , Volumen de Ventilación Pulmonar/genética , Ventilación/normas
2.
Sci Rep ; 10(1): 5118, 2020 03 20.
Artículo en Inglés | MEDLINE | ID: mdl-32198404

RESUMEN

Tenascin-C (TNC) is an extracellular matrix protein expressed at high levels during lung organogenesis. Later, TNC is only transiently de novo expressed to orchestrate tissue repair in pathological situations. We previously showed that TNC inactivation affects lung development and thus evaluated here the implications on lung function in newborn/adult mice. Respiratory function parameters were measured in anesthetized and mechanically ventilated wild-type (WT) and TNC-deficient mice at 5 (P5) and 90 (P90) days of age under basal conditions, as well as following high tidal volume (HTV) ventilation. At P5, TNC-deficient mice showed an increased static compliance (Cst) and inspiratory capacity (IC) relative to WT at baseline and throughout HTV. At P90, however, Cst and IC were only elevated at baseline. Control non-ventilated newborn and adult TNC-deficient mice showed similar lung morphology, but less alpha smooth muscle actin (α-SMA) around small airways. SMA + cells were decreased by 50% in adult TNC-deficient lungs and collagen layer thickened around small airways. Increased surfactant protein C (SP-C) and altered TGFß and TLR4 signaling pathways were also detected. Thus, TNC inactivation-related defects during organogenesis led to persisting functional impairment in adulthood. This might be of interest in the context of pulmonary diseases with thickened airway smooth muscle layer or ventilation heterogeneity, like asthma and COPD.


Asunto(s)
Pulmón/crecimiento & desarrollo , Pulmón/patología , Organogénesis/fisiología , Tenascina/metabolismo , Volumen de Ventilación Pulmonar/fisiología , Actinas/metabolismo , Animales , Proliferación Celular , Colágeno/metabolismo , Matriz Extracelular/metabolismo , Pulmón/anatomía & histología , Ratones , Ratones Noqueados , Organogénesis/genética , Proteína C Asociada a Surfactante Pulmonar/metabolismo , Respiración Artificial , Transducción de Señal/fisiología , Tenascina/genética , Volumen de Ventilación Pulmonar/genética , Receptor Toll-Like 4/metabolismo , Factor de Crecimiento Transformador beta/metabolismo
3.
Artículo en Inglés | MEDLINE | ID: mdl-31195123

RESUMEN

The "jet stream" model predicts an expired flow within the dorsal part of the buccal cavity with small air mixing during buccal pump ventilation, and has been suggested for some anuran amphibians but no other species of air breathing animal using a buccal force pump has been investigated. The presence of a two-stroke buccal pump in lungfish, i.e. expiration followed by inspiration, was described previously, but no quantitative data are available for the dead-space of their respiratory system and neither a detailed description of airflow throughout a breathing cycle. The present study aimed to assess the degree of mixing of fresh air and expired gas during the breathing cycle of Lepidosiren paradoxa and to verify the possible presence of a jet stream during expiration in this species. To do so, simultaneous measurements of buccal pressure and ventilatory airflows were carried out. Buccal and lung gases (PCO2 and PO2) were also measured. The effective ventilation was calculated and the dead space estimated using Bohr equations. The results confirmed that the two-stroke buccal pump is present in lungfish, as it is in anuran amphibians. The present approaches were coherent with a small dead space, with a very small buccal-lung PCO2 difference. In the South American lungfish the dead space (VD) as a percentage of tidal volume (VT) (VD / VT) ranged from 4.1 to 12.5%. Our data support the presence of a jet stream and indicate a small degree of air mixing in the buccal cavity. Comparisons with the literature indicate that these data are similar to previous data reported for the toad Rhinella schneideri.


Asunto(s)
Mejilla/fisiología , Pulmón/fisiología , Perciformes/fisiología , Intercambio Gaseoso Pulmonar/fisiología , Animales , Dióxido de Carbono/metabolismo , Oxígeno/metabolismo , Perciformes/genética , Intercambio Gaseoso Pulmonar/genética , Respiración , Volumen de Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/fisiología
4.
Am J Respir Cell Mol Biol ; 60(5): 569-577, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-30428271

RESUMEN

The aim of this study was to assess the association between regional tidal volume (Vt), regional functional residual capacity (FRC), and the expression of genes linked with ventilator-induced lung injury. Two groups of BALB/c mice (n = 8 per group) were ventilated for 2 hours using a protective or injurious ventilation strategy, with free-breathing mice used as control animals. Regional Vt and FRC of the ventilated mice was determined by analysis of high-resolution four-dimensional computed tomographic images taken at baseline and after 2 hours of ventilation and corrected for the volume of the region (i.e., specific [s]Vt and specific [s]FRC). RNA concentrations of 21 genes in 10 different lung regions were quantified using a quantitative PCR array. sFRC at baseline varied regionally, independent of ventilation strategy, whereas sVt varied regionally depending on ventilation strategy. The expression of IL-6 (P = 0.04), Ccl2 (P < 0.01), and Ang-2 (P < 0.05) was associated with sVt but not sFRC. The expression of seven other genes varied regionally (IL-1ß and RAGE [receptor for advanced glycation end products]) or depended on ventilation strategy (Nfe2l2 [nuclear factor erythroid-derived 2 factor 2], c-fos, and Wnt1) or both (TNF-α and Cxcl2), but it was not associated with regional sFRC or sVt. These observations suggest that regional inflammatory responses to mechanical ventilation are driven primarily by tidal stretch.


Asunto(s)
Fenómenos Biomecánicos/inmunología , Regulación de la Expresión Génica/inmunología , Pulmón/inmunología , Respiración Artificial/métodos , Lesión Pulmonar Inducida por Ventilación Mecánica/genética , Animales , Líquido del Lavado Bronquioalveolar/química , Líquido del Lavado Bronquioalveolar/inmunología , Quimiocina CCL2/genética , Quimiocina CCL2/inmunología , Quimiocina CXCL2/genética , Quimiocina CXCL2/inmunología , Tomografía Computarizada Cuatridimensional , Interpretación de Imagen Asistida por Computador , Interleucina-1beta/genética , Interleucina-1beta/inmunología , Interleucina-6/genética , Interleucina-6/inmunología , Pulmón/diagnóstico por imagen , Pulmón/fisiopatología , Masculino , Ratones , Ratones Endogámicos BALB C , Factor 2 Relacionado con NF-E2/genética , Factor 2 Relacionado con NF-E2/inmunología , Proteínas Proto-Oncogénicas c-fos/genética , Proteínas Proto-Oncogénicas c-fos/inmunología , Receptor para Productos Finales de Glicación Avanzada/genética , Receptor para Productos Finales de Glicación Avanzada/inmunología , Ribonucleasa Pancreática/genética , Ribonucleasa Pancreática/inmunología , Transducción de Señal , Volumen de Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/inmunología , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/inmunología , Lesión Pulmonar Inducida por Ventilación Mecánica/diagnóstico por imagen , Lesión Pulmonar Inducida por Ventilación Mecánica/inmunología , Lesión Pulmonar Inducida por Ventilación Mecánica/fisiopatología , Proteína Wnt1/genética , Proteína Wnt1/inmunología
5.
Respir Physiol Neurobiol ; 248: 48-54, 2018 01.
Artículo en Inglés | MEDLINE | ID: mdl-29208534

RESUMEN

Cyclin-dependent kinase-like 5 (CDKL5) is an X-linked gene encoding a serine-threonine kinase that is highly expressed in the central nervous system. Mutations in CDKL5 cause neurological and psychiatric symptoms, including early-onset seizures, motor dysfunction, autistic features and sleep breathing abnormalities in patients. It remains to be addressed whether loss of CDKL5 causes respiratory dysfunction in mice. Here, we examined the respiratory pattern of male Cdkl5-/y mice at 1-3 months of age during resting breathing and respiratory challenge (i.e., hypoxia and hypercapnia) via whole body plethysmography. The results demonstrated that the resting respiratory frequency and tidal volume of Cdkl5-/y mice was unaltered compared to that of WT mice at 1 month of age. However, these mutant mice exhibit transient reduction in tidal volume during respiratory challenge even the reduction was restored at 2 months of age. Notably, the sigh-breathing pattern was changed in Cdkl5-/y mice, showing a transient reduction in sigh volume at 1-2 month of age and long-term attenuation of peak expiratory airflow from 1 to 3 month of age. Therefore, loss of CDKL5 causes breathing deficiency, supporting a CDKL5-mediated regulation of respiratory function in mice.


Asunto(s)
Proteínas Serina-Treonina Quinasas/deficiencia , Trastornos Respiratorios/genética , Factores de Edad , Animales , Peso Corporal/genética , Genotipo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Pletismografía , Proteínas Serina-Treonina Quinasas/genética , Ventilación Pulmonar/genética , Trastornos Respiratorios/fisiopatología , Volumen de Ventilación Pulmonar/genética
6.
Acta Neuropathol Commun ; 4: 23, 2016 Mar 02.
Artículo en Inglés | MEDLINE | ID: mdl-26936605

RESUMEN

INTRODUCTION: Guillain-Barré syndrome (GBS) is an autoimmune disease that results in acute paralysis through inflammatory attack on peripheral nerves, and currently has limited, non-specific treatment options. The pathogenesis of the acute motor axonal neuropathy (AMAN) variant is mediated by complement-fixing anti-ganglioside antibodies that directly bind and injure the axon at sites of vulnerability such as nodes of Ranvier and nerve terminals. Consequently, the complement cascade is an attractive target to reduce disease severity. Recently, C5 complement component inhibitors that block the formation of the membrane attack complex and subsequent downstream injury have been shown to be efficacious in an in vivo anti-GQ1b antibody-mediated mouse model of the GBS variant Miller Fisher syndrome (MFS). However, since gangliosides are widely expressed in neurons and glial cells, injury in this model was not targeted exclusively to the axon and there are currently no pure mouse models for AMAN. Additionally, C5 inhibition does not prevent the production of early complement fragments such as C3a and C3b that can be deleterious via their known role in immune cell and macrophage recruitment to sites of neuronal damage. RESULTS AND CONCLUSIONS: In this study, we first developed a new in vivo transgenic mouse model of AMAN using mice that express complex gangliosides exclusively in neurons, thereby enabling specific targeting of axons with anti-ganglioside antibodies. Secondly, we have evaluated the efficacy of a novel anti-C1q antibody (M1) that blocks initiation of the classical complement cascade, in both the newly developed anti-GM1 antibody-mediated AMAN model and our established MFS model in vivo. Anti-C1q monoclonal antibody treatment attenuated complement cascade activation and deposition, reduced immune cell recruitment and axonal injury, in both mouse models of GBS, along with improvement in respiratory function. These results demonstrate that neutralising C1q function attenuates injury with a consequent neuroprotective effect in acute GBS models and promises to be a useful new target for human therapy.


Asunto(s)
Complemento C1q/metabolismo , Vía Clásica del Complemento/fisiología , Gangliósidos/metabolismo , Enfermedades del Sistema Nervioso Periférico/patología , Animales , Anticuerpos/farmacología , Anticuerpos/uso terapéutico , Complemento C1q/genética , Vía Clásica del Complemento/genética , Diafragma/metabolismo , Diafragma/patología , Transportadores de Ácidos Dicarboxílicos/genética , Modelos Animales de Enfermedad , Gangliósidos/clasificación , Gangliósidos/inmunología , Síndrome de Guillain-Barré/metabolismo , Síndrome de Guillain-Barré/patología , Humanos , Infiltración Leucémica , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Transgénicos , Actividad Motora/efectos de los fármacos , Actividad Motora/genética , Enfermedades del Sistema Nervioso Periférico/tratamiento farmacológico , Enfermedades del Sistema Nervioso Periférico/genética , Enfermedades del Sistema Nervioso Periférico/fisiopatología , Receptores Nicotínicos/metabolismo , Respiración/efectos de los fármacos , Respiración/genética , Especificidad de la Especie , Simportadores/genética , Volumen de Ventilación Pulmonar/efectos de los fármacos , Volumen de Ventilación Pulmonar/genética
7.
Respir Physiol Neurobiol ; 208: 29-36, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25596543

RESUMEN

Prenatal nicotine exposure alters breathing and ventilatory responses to stress through stimulation of nicotine acetylcholine receptors (nAChRs). We tested the hypothesis that α4-containing nAChRs are involved in mediating the effects of prenatal nicotine exposure on ventilatory and metabolic responses to intermittent mild asphyxia (MA). Using open-flow plethysmography, we measured ventilation (V̇(E)) and rate of O2 consumption ( V̇(O2)) of wild-type (WT) and α4-knock-out (KO) mice, at postnatal (P) days 1-2 and 7-8, with and without prenatal nicotine exposure (6 mg kg(-1) day(-1) beginning on embryonic day 14). Mice were exposed to seven 2 min cycles of mild asphyxia (10% O2 and 5% CO2), each interspersed with 2 min of air. Compared to WT, α4 KO mice had increased air V̇(E) and V̇(O2) at P7-8, but not P1-2. Irrespective of age, genotype had no effect on the hyperventilatory response (increase in V̇(E)/V̇(O2)) to MA. At P1-2, nicotine suppressed air V̇(E) and V̇(O2) in both genotypes but did not affect the hyperventilatory response to MA. At P7-8 nicotine suppressed air V̇(E) and V̇(O2) of only α4 KO's but also significantly enhanced V̇(E) during MA (nearly double that of WT; p<0.001). This study has revealed complex effects of α4 nAChR deficiency and prenatal nicotine exposure on ventilatory and metabolic interactions and responses to stress.


Asunto(s)
Asfixia Neonatal/fisiopatología , Hiperventilación/inducido químicamente , Hiperventilación/genética , Efectos Tardíos de la Exposición Prenatal/genética , Efectos Tardíos de la Exposición Prenatal/fisiopatología , Receptores Nicotínicos/deficiencia , Factores de Edad , Análisis de Varianza , Animales , Animales Recién Nacidos , Femenino , Recién Nacido de Bajo Peso/fisiología , Masculino , Ratones , Ratones Noqueados , Consumo de Oxígeno/efectos de los fármacos , Consumo de Oxígeno/genética , Embarazo , Receptores Nicotínicos/genética , Respiración/efectos de los fármacos , Respiración/genética , Volumen de Ventilación Pulmonar/efectos de los fármacos , Volumen de Ventilación Pulmonar/genética , Factores de Tiempo
8.
Neuroscience ; 267: 166-76, 2014 May 16.
Artículo en Inglés | MEDLINE | ID: mdl-24626160

RESUMEN

Respiratory disturbances are a primary phenotype of the neurological disorder, Rett syndrome (RTT), caused by mutations in the X-linked gene encoding methyl-CpG-binding protein 2 (MeCP2). Mouse models generated with null mutations in Mecp2 mimic respiratory abnormalities in RTT girls. Large deletions, however, are seen in only ∼10% of affected human individuals. Here we characterized respiration in heterozygous females from two mouse models that genetically mimic common RTT point mutations, a missense mutation T158A (Mecp2(T158A/)(+)) or a nonsense mutation R168X (Mecp2(R168X/+)). MeCP2 T158A shows decreased binding to methylated DNA, while MeCP2 R168X retains the capacity to bind methylated DNA but lacks the ability to recruit complexes required for transcriptional repression. We found that both Mecp2(T158A/+) and Mecp2(R168X/+) heterozygotes display augmented hypoxic ventilatory responses and depressed hypercapnic responses, compared to wild-type controls. Interestingly, the incidence of apnea was much greater in Mecp2(R168X/+) heterozygotes, 189 per hour, than Mecp2(T158A/+) heterozygotes, 41 per hour. These results demonstrate that different RTT mutations lead to distinct respiratory phenotypes, suggesting that characterization of the respiratory phenotype may reveal functional differences between MeCP2 mutations and provide insights into the pathophysiology of RTT.


Asunto(s)
Proteína 2 de Unión a Metil-CpG/genética , Mutación/genética , Trastornos Respiratorios/etiología , Síndrome de Rett/complicaciones , Síndrome de Rett/genética , Factores de Edad , Animales , Dióxido de Carbono/farmacología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Femenino , Hipoxia/fisiopatología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Pletismografía , Trastornos Respiratorios/genética , Volumen de Ventilación Pulmonar/genética
9.
Curr Opin Crit Care ; 18(1): 16-22, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22157252

RESUMEN

PURPOSE OF REVIEW: Ventilator-induced lung injury (VILI) is a ubiquitous iatrogenic clinical problem in critical care. Aside from avoiding large tidal volumes, little progress has been made in identifying effective clinical strategies to minimize this injury. With recent rapid development in bioinformatics and high-throughput molecular technology, the genetic basis of lung injury has been intensively investigated. This review will describe recent insights and potential therapies developed in the field. RECENT FINDINGS: Much progress has been made in delineating the possible genes and gene products involved in VILI through various mechanisms such as early induced genes, capillary leak, apoptosis, fibrin deposition, inflammatory cytokines, oxidative stress, disrupted angiogenesis, and neutrophil infiltration. Some studies have translated bench findings to the bedside in an attempt to identify clinically important genetic susceptibility, which could aid in the identification of at-risk individuals who might benefit from careful titration of mechanical ventilation. Genetic insights also provide candidate pharmaceutical approaches that may ameliorate VILI in the future. SUMMARY: Much relevant information exists for investigators and clinicians interested in VILI. Future research will interlink evolving data to provide a more integrated picture of the molecular mechanisms involved in VILI enabling translation of the most promising candidate therapies.


Asunto(s)
Activación Transcripcional , Lesión Pulmonar Inducida por Ventilación Mecánica/genética , Ventiladores Mecánicos/efectos adversos , Animales , Expresión Génica , Predisposición Genética a la Enfermedad/genética , Humanos , ARN Mensajero/genética , Volumen de Ventilación Pulmonar/genética , Lesión Pulmonar Inducida por Ventilación Mecánica/prevención & control
10.
Respir Physiol Neurobiol ; 170(2): 173-82, 2010 Feb 28.
Artículo en Inglés | MEDLINE | ID: mdl-20040383

RESUMEN

Rett syndrome is a neurodevelopmental disease accompanied by complex, disabling symptoms, including breathing symptoms. Because Rett syndrome is caused by mutations in the transcriptional repressor methyl-CpG-binding protein 2 (MeCP2), Mecp2-deficient mice have been generated as experimental model. Males of Mecp2-deficient mice (Mecp2(-/y)) breathe normally at birth but show abnormal respiratory responses to hypoxia and hypercapnia from postnatal day 25 (P25). After P30, Mecp2(-/y) mice develop breathing symptoms reminiscent of Rett syndrome, aggravating until premature death at around P60. Using plethysmography, we analyzed the sighs and the post-sigh breathing pattern of unrestrained wild type male mice (WT) and Mecp2(-/y) mice from P15 to P60. Sighs are spontaneous large inspirations known to prevent lung atelectasis and to improve alveolar oxygenation. However, Mecp2(-/y) mice show early abnormalities of post-sigh breathing, with long-lasting post-sigh apnoeas, reduced tidal volume when eupnoea resumes and lack of post-sigh bradypnoea which develop from P15, aggravate with age and possibly contribute to breathing symptoms to come.


Asunto(s)
Hipercalcemia/complicaciones , Hipoxia/complicaciones , Proteína 2 de Unión a Metil-CpG/deficiencia , Anomalías del Sistema Respiratorio/fisiopatología , Síndrome de Rett/fisiopatología , Factores de Edad , Animales , Animales Recién Nacidos , Apnea/genética , Apnea/fisiopatología , Modelos Animales de Enfermedad , Hipercalcemia/genética , Hipoxia/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Pletismografía , Respiración/genética , Mecánica Respiratoria/genética , Anomalías del Sistema Respiratorio/genética , Síndrome de Rett/genética , Volumen de Ventilación Pulmonar/genética
11.
Respir Physiol Neurobiol ; 165(1): 40-8, 2009 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-18977317

RESUMEN

A mouse strain with a deleted acetylcholinesterase (AChE) gene (AChE knockout) shows a decreased inspiration time and increased tidal volume and ventilation .To investigate the respective roles of AChE in brain and muscle, we recorded respiration by means of whole-body plethysmography in knockout mice with tissue selective deletions in AChE expression. A mouse strain with the anchoring domains of AChE deleted (del E5+6 knockout mice) has very low activity in the brain and neuromuscular junction, but increased monomeric AChE in serum. A mouse strain with deletion of the muscle specific region of AChE (del i1RR knockout mice) exhibits no expression in muscle, but unaltered expression in the central nervous system. Neither strain exhibits the pronounced phenotypic traits observed in the complete AChE knockout strain. A third strain lacking the anchor molecule PRiMA, has no functional AChE and butyrylcholinesterase (BChE) in brain and an unaltered respiratory function. BChE inhibition by bambuterol decreases tidal volume and body temperature in del E5+6 and i1RR knockout strains, but not in PRiMA deletion or wild-type controls. We find that: (1) deletion of the full AChE gene is required for a pronounced alteration in respiratory phenotype, (2) BChE is involved in respiratory muscles contraction and temperature control in del E5+6 and i1RR knockout mice, and (3) AChE expression requiring a gene product splice to either exons 5 and 6 or regulated by intron1 influences temperature control.


Asunto(s)
Acetilcolinesterasa/metabolismo , Encéfalo/enzimología , Regulación de la Expresión Génica/fisiología , Músculos/enzimología , Respiración/genética , Acetilcolinesterasa/deficiencia , Análisis de Varianza , Animales , Temperatura Corporal/genética , Encéfalo/efectos de los fármacos , Broncodilatadores/farmacología , Butirilcolinesterasa/metabolismo , Exones/genética , Femenino , Regulación de la Expresión Génica/genética , Hipercapnia/genética , Hipercapnia/fisiopatología , Hipoxia/genética , Hipoxia/fisiopatología , Masculino , Ratones , Ratones Noqueados , Músculos/efectos de los fármacos , Pletismografía Total/métodos , Ventilación Pulmonar/genética , Respiración/efectos de los fármacos , Eliminación de Secuencia/genética , Terbutalina/análogos & derivados , Terbutalina/farmacología , Volumen de Ventilación Pulmonar/genética
12.
Am J Physiol Heart Circ Physiol ; 295(1): H59-68, 2008 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-18456734

RESUMEN

Recent studies have suggested a genetic component to heart rate (HR) and HR variability (HRV). However, a systematic examination of the genetic contribution to the variation in HR and HRV has not been performed. This study investigated the genetic contribution to HR and HRV using a wide range of inbred and recombinant inbred (RI) mouse strains. Electrocardiogram data were recorded from 30 strains of inbred mice and 29 RI strains. Significant differences in mean HR and total power (TP) HRV were identified between inbred strains and RI strains. Multiple significant differences within the strain sets in mean low-frequency (LF) and high-frequency (HF) power were also found. No statistically significant concordance was found between strain distribution patterns for HR and HRV phenotypes. Genomewide interval mapping identified a significant quantitative trait locus (QTL) for HR [LOD (likelihood of the odds) score = 3.763] on chromosome 6 [peak at 53.69 megabases (Mb); designated HR 1 (Hr1)]. Suggestive QTLs for TP were found on chromosomes 2, 4, 5, 6, and 14. A suggestive QTL for LF was found on chromosome 16; for HF, we found one significant QTL on chromosome 5 (LOD score = 3.107) [peak at 53.56 Mb; designated HRV-high-frequency 1 (Hrvhf1)] and three suggestive QTLs on chromosomes 2, 11 and 15. In conclusion, the results demonstrate a strong genetic component in the regulation of resting HR and HRV evidenced by the significant differences between strains. A lack of correlation between HR and HRV phenotypes in some inbred strains suggests that different sets of genes control the phenotypes. Furthermore, QTLs were found that will provide important insight to the genetic regulation of HR and HRV at rest.


Asunto(s)
Frecuencia Cardíaca/genética , Animales , Mapeo Cromosómico , Electrocardiografía Ambulatoria , Genotipo , Escala de Lod , Masculino , Ratones , Ratones Endogámicos , Fenotipo , Ventilación Pulmonar/genética , Sitios de Carácter Cuantitativo , Mecánica Respiratoria/genética , Especificidad de la Especie , Telemetría , Volumen de Ventilación Pulmonar/genética
13.
Am J Physiol Regul Integr Comp Physiol ; 290(6): R1691-6, 2006 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-16410396

RESUMEN

Heterozygous mutations of the transcription factor PHOX2B have been found in most patients with central congenital hypoventilation syndrome, a rare disease characterized by sleep-related hypoventilation and impaired chemosensitivity to sustained hypercapnia and sustained hypoxia. PHOX2B is a master regulator of autonomic reflex pathways, including peripheral chemosensitive pathways. In the present study, we used hyperoxic tests to assess the strength of the peripheral chemoreceptor tonic drive in Phox2b+/-newborn mice. We exposed 69 wild-type and 67 mutant mice to two hyperoxic tests (12-min air followed by 3-min 100% O2) 2 days after birth. Breathing variables were measured noninvasively using whole body flow plethysmography. The initial minute ventilation decrease was larger in mutant pups than in wild-type pups: -37% (SD 13) and -25% (SD 18), respectively, P<0.0001. Furthermore, minute ventilation remained depressed throughout O2 exposure in mutants, possibly because of their previously reported impaired CO2 chemosensitivity, whereas it returned rapidly to the normoxic level in wild-type pups. Hyperoxia considerably increased total apnea duration in mutant compared with wild-type pups (P=0.0001). A complementary experiment established that body temperature was not influenced by hyperoxia in either genotype group and, therefore, did not account for genotype-related differences in the hyperoxic ventilatory response. Thus partial loss of Phox2b function by heterozygosity did not diminish the tonic drive from peripheral chemoreceptors.


Asunto(s)
Proteínas de Homeodominio/genética , Hiperoxia/fisiopatología , Ventilación Pulmonar/fisiología , Factores de Transcripción/genética , Animales , Animales Recién Nacidos , Apnea/genética , Apnea/fisiopatología , Temperatura Corporal/genética , Temperatura Corporal/fisiología , Femenino , Heterocigoto , Masculino , Ratones , Ratones Mutantes , Pletismografía Total , Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/fisiología
14.
Respir Physiol Neurobiol ; 152(1): 92-9, 2006 May.
Artículo en Inglés | MEDLINE | ID: mdl-16095981

RESUMEN

Opioids inhibit breathing in mammals, especially in newborns, and are also implicated in the control of hypoxic anapyrexia. We measured breathing patterns and metabolic responses to 12% oxygen in six adult male wildtype C57B/6J mice and six preproenkephalin knockout (PPNK-/-) mice in a flow-through respirometer and barometric plethysmograph with ambient temperature maintained in the thermoneutral zone. Breathing air, there was no significant difference between the two groups of mice in ventilation ((.)V), oxygen consumption ((.)V(O(2)), convection requirement ((.)V/(.)V(O(2)), tidal volume (V(t)), frequency (f), or inspiratory time (T(i)); however, PPNK-/- mice had a significantly shorter expiratory time (T(e)). The breathing pattern response to 5% CO(2) was the same between wildtype and PPNK-/- in terms of absolute values, but the % change in V(t) was greater in the wildtype. Breathing 12% O(2), there was no significant difference in V , V(t), f, T(i), T(e) or body temperature between groups, but there was a significant difference in (.)V(O(2) (PPNK-/- 1.24+/-0.05 ml O(2)min(-1) versus 0.91+/-0.05 for wildtype, P<0.001) and % change in (.)V(O(2), (2.3+/-6.6% for PPNK-/- versus -28+/-3.8% for wildtype); in ((.)V/(.)V(O(2)), (54+/-4 versus 78+/-10, P<0.05) and the % change in (.)V/(.)V(O(2), (37+/-9 versus 131+/-28, P<0.01). These data implicate enkephalin as a signaling molecule in the control of hypoxic depression of metabolism in mice.


Asunto(s)
Encefalinas/deficiencia , Hipoxia/metabolismo , Hipoxia/fisiopatología , Precursores de Proteínas/deficiencia , Respiración/genética , Animales , Análisis de los Gases de la Sangre/métodos , Temperatura Corporal/genética , Hipoxia/genética , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Consumo de Oxígeno/fisiología , Pletismografía/métodos , Intercambio Gaseoso Pulmonar/genética , Volumen de Ventilación Pulmonar/genética
15.
Respir Physiol Neurobiol ; 145(2-3): 143-51, 2005 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-15705530

RESUMEN

In order to determine the role of Kir2.2 in the hypercapnic ventilatory response (HCVR) during postnatal development, we measured the response of the Kir2.2-knockout (Kir2.2-/-) mouse in an unanesthetized unrestrained state by means of pressure plethysmography on postnatal days 9-10, 14-15 and 18, and compared the response with that in its wild counterpart, the FVB mouse. We also examined developmental changes in m-RNA expression of Kir2.2 in the brainstem of the FVB mouse using quantitative real-time PCR assay. Kir2.2-/- exhibited a smaller increase in tidal volume and minute ventilation volume than the FVB mouse in response to hypercapnic challenge on days 14-15. Meanwhile, the FVB mouse showed a transient increase in m-RNA expression of Kir2.2 in the brainstem on days 14-15. These findings suggest that Kir2.2 in the brainstem plays a transient role in HCVR, possibly through central ventilatory chemosensitivity, during postnatal development.


Asunto(s)
Animales Recién Nacidos/crecimiento & desarrollo , Hipercapnia/fisiopatología , Canales de Potasio de Rectificación Interna/fisiología , Respiración , Factores de Edad , Análisis de Varianza , Animales , Southern Blotting/métodos , Tronco Encefálico/crecimiento & desarrollo , Tronco Encefálico/metabolismo , Regulación del Desarrollo de la Expresión Génica/fisiología , Genotipo , Hipercapnia/genética , Ratones , Ratones Noqueados , Fenotipo , Pletismografía/métodos , Canales de Potasio de Rectificación Interna/deficiencia , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Volumen de Ventilación Pulmonar/genética , Vigilia/fisiología
16.
Respir Physiol Neurobiol ; 139(3): 237-45, 2004 Feb 25.
Artículo en Inglés | MEDLINE | ID: mdl-15122990

RESUMEN

Acetylcholine (ACh) acting through muscarinic receptors is thought to be involved in the control of breathing, notably in central and peripheral chemosensory afferents and in regulations related to sleep-wake states. By using whole-body plethysmography, we compared baseline breathing at rest and ventilatory responses to acute exposure (5 min) to moderate hypoxia (10% O(2)) and hypercapnia (3 and 5% CO(2)) in mice lacking either the M(1) or the M(3) muscarinic receptor, and in wild-type matched controls. M(1) knockout mice showed normal minute ventilation (V(E)) but elevated tidal volume (V(T)) at rest, and normal chemosensory ventilatory responses to hypoxia and hypercapnia. M(3) knockout mice had elevated V(E) and V(T) at rest, a reduced V(T) response slope to hypercapnia, and blunted V(E) and frequency responses to hypoxia. The results suggest that M(1) and M(3) muscarinic receptors play significant roles in the regulation of tidal volume at rest and that the afferent pathway originating from peripheral chemoreceptors involves M(3) receptors.


Asunto(s)
Hipercapnia/fisiopatología , Hipoxia/fisiopatología , Ventilación Pulmonar/fisiología , Receptor Muscarínico M1/fisiología , Receptor Muscarínico M3/fisiología , Respiración/genética , Animales , Temperatura Corporal/genética , Temperatura Corporal/fisiología , Peso Corporal/genética , Peso Corporal/fisiología , Dióxido de Carbono/farmacología , Relación Dosis-Respuesta a Droga , Ratones , Ratones Endogámicos , Ratones Noqueados , Oxígeno/farmacología , Pletismografía Total/métodos , Ventilación Pulmonar/efectos de los fármacos , Receptor Muscarínico M1/genética , Receptor Muscarínico M3/genética , Respiración/efectos de los fármacos , Volumen de Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/fisiología
17.
Respir Physiol Neurobiol ; 130(1): 33-42, 2002 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-12380014

RESUMEN

The effects of a deficiency of glycinergic inhibition deriving from mutations of the glycine-receptor gene Glra1 on the breathing pattern of oscillator mice were studied. We compared the development of breathing frequency, tidal volume and minute ventilation from control mice (wild type- and heterozygous oscillator mice) with those of homozygous oscillator mice during early postnatal periods from p9 until p21. The changes of ventilation were correlated with body-weight and changes in blood-pH. During the second to third weeks of postnatal development, breathing frequency increased from 310 to 445.4 mm-1 in control mice. Oscillator mice reached a maximal value of 313.3 min-1 at p18 followed by a fast decrease to 233.0 min-1. This decrease is caused by a prolongation of expiratory duration. Tidal volume showed a steady increase from 6.6 to 15.1 microliters in control animals. In comparison, oscillator mice showed significant lower values after p14. After p15, minute ventilation of oscillator mice declined as compared with control animals leading to respiratory acidosis at p20.


Asunto(s)
Relojes Biológicos/genética , Receptores de Glicina/deficiencia , Receptores de Glicina/genética , Respiración/genética , Animales , Animales Recién Nacidos/genética , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Isoformas de Proteínas/deficiencia , Isoformas de Proteínas/genética , Ventilación Pulmonar/genética , Receptores de Glicina/fisiología , Volumen de Ventilación Pulmonar/genética
18.
J Neurosci ; 21(14): 5212-21, 2001 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-11438596

RESUMEN

The abnormal metabolism of serotonin during the perinatal period alters respiratory network maturation at birth as revealed by comparing the monoamine oxidase A-deficient transgenic (Tg8) with the control (C3H) mice (Bou-Flores et al., 2000). To know whether these alterations occur only transiently or induce persistent respiratory dysfunction during adulthood, we studied the respiratory activity and regulations in adult C3H and Tg8 mice. First, plethysmographic and pneumotachographic analyses of breathing patterns revealed weaker tidal volumes and shorter inspiratory durations in Tg8 than in C3H mice. Second, electrophysiological studies showed that the firing activity of inspiratory medullary neurons and phrenic motoneurons is higher in Tg8 mice and that of the intercostal motoneurons in C3H mice. Third, histological studies indicated abnormally large cell bodies of Tg8 intercostal but not phrenic motoneurons. Finally, respiratory responses to hypoxia and lung inflation are weaker in Tg8 than in C3H mice. dl-p-chlorophenyl-alanine treatments applied to Tg8 mice depress the high serotonin level present during adulthood; the treated mice recover normal respiratory responses to both hypoxia and lung inflation, but their breathing parameters are not significantly affected. Therefore in Tg8 mice the high serotonin level occurring during the perinatal period alters respiratory network maturation and produces a permanent respiratory dysfunction, whereas the high serotonin level present in adults alters the respiratory regulatory processes. In conclusion, the metabolism of serotonin plays a crucial role in the maturation of the respiratory network and in both the respiratory activity and the respiratory regulations.


Asunto(s)
Monoaminooxidasa/deficiencia , Trastornos Respiratorios/fisiopatología , Animales , Tamaño de la Célula/genética , Electrofisiología , Fenclonina/farmacología , Hipoxia/fisiopatología , Nervios Intercostales , Bulbo Raquídeo/efectos de los fármacos , Bulbo Raquídeo/patología , Bulbo Raquídeo/fisiopatología , Ratones , Ratones Endogámicos C3H , Ratones Transgénicos , Monoaminooxidasa/genética , Monoaminooxidasa/metabolismo , Neuronas Motoras/citología , Neuronas Motoras/efectos de los fármacos , Neuronas Motoras/metabolismo , Red Nerviosa/efectos de los fármacos , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Neuronas/patología , Nervio Frénico , Pletismografía , Reflejo/efectos de los fármacos , Reflejo/genética , Respiración/efectos de los fármacos , Respiración/genética , Trastornos Respiratorios/tratamiento farmacológico , Trastornos Respiratorios/genética , Serotonina/metabolismo , Volumen de Ventilación Pulmonar/genética
19.
J Appl Physiol (1985) ; 89(2): 413-21, 2000 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10926621

RESUMEN

Researchers investigating the genetic component of various disease states rely increasingly on murine models. We have developed a ventilator to simplify respiratory research in small animals down to murine size. The new ventilator provides constant-flow inflation and tidal volume delivery independent of respiratory parameter changes. The inclusion of end-inspiratory and end-expiratory pauses simplifies the measurement of airway resistance and compliance and allows the detection of dynamic hyperinflation (auto-positive end-expiratory pressure). After bench testing, we performed intravenous methacholine challenge on two strains of mice (A/J and C57bl/bj) known to differ in their responses by using the new ventilator. Dynamic hyperinflation and a decrease in compliance developed during methacholine challenge whenever respiratory rates of 60-120 breaths/min were employed. In contrast, if dynamic hyperinflation was prevented by lengthening expiratory time, (respiratory rate = 20 breaths/min), static compliance remained constant. More importantly, the coefficient of variation of the results decreased when lung volume shifts were prevented. In conclusion, airway challenge studies have greater precision when dynamic hyperinflation is prevented.


Asunto(s)
Pulmón/fisiología , Pruebas de Función Respiratoria/instrumentación , Mecánica Respiratoria/fisiología , Ventiladores Mecánicos , Presión del Aire , Resistencia de las Vías Respiratorias/genética , Resistencia de las Vías Respiratorias/fisiología , Animales , Hiperreactividad Bronquial/fisiopatología , Pulmón/efectos de los fármacos , Rendimiento Pulmonar/efectos de los fármacos , Rendimiento Pulmonar/genética , Rendimiento Pulmonar/fisiología , Cloruro de Metacolina , Ratones , Ratones Endogámicos A , Ratones Endogámicos C57BL , Mecánica Respiratoria/efectos de los fármacos , Mecánica Respiratoria/genética , Especificidad de la Especie , Volumen de Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/fisiología
20.
Adv Exp Med Biol ; 475: 75-85, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-10849650

RESUMEN

Inbred mice are routinely used as genetic models in lung biology. Among many phenotypic differences in lung function and structure, C3H/HeJ (C3) and C57BL/6J (B6) inbred mice also demonstrate a significantly different ventilatory pattern during acute hypoxic challenge. The present study rejects the hypothesis that a genomic basis for differential hypoxic ventilatory responses (HVR) is linked to loci which determine differential breathing pattern at baseline, while proposing an alternative genetic model for HVR variation. Twelve BXH recombinant inbred (RI) strains derived from C3 and B6 progenitors were examined to enumerate the genes regulating differential HVR. In each of 134 mice, HVR was assessed using whole-body plethysmography to measure tidal volume (VT) and breathing frequency (f). With respect to f during hypoxia, three distinct and reproducible phenotypes are evident in the BXH RI strain distribution pattern (SDP). The SDP for hypoxic f is consistent with the hypothesis that parental strain differences are regulated by two genes. Cosegregation analysis suggest that the genetic control of f during hypoxia differs from the genes which control differential baseline f. Although the genetic control of VT appears more complex, differences in the minute ventilation (VE) during hypoxia is determined by VT. Therefore, this study suggests that the phenotypic variation in HVR between C3 and B6 parental strains, especially related to f during hypoxia, is regulated by as few as two major genetic determinants.


Asunto(s)
Hipoxia/genética , Hipoxia/fisiopatología , Modelos Genéticos , Respiración/genética , Animales , Humanos , Masculino , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Fenotipo , Recombinación Genética , Especificidad de la Especie , Volumen de Ventilación Pulmonar/genética , Volumen de Ventilación Pulmonar/fisiología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...