RESUMEN
Plant laccases have been proposed to participate in lignin biosynthesis. However, there is no direct evidence that individual laccases in Populus can polymerize lignin monomers and alter cell wall structure. Here, a Populus laccase, PtrLAC16, was expressed and purified in a eukaryotic system. Enzymatic analysis of PtrLAC16 showed that it could polymerize lignin monomers in vitro. PtrLAC16 preferred sinapyl alcohol, and this preference is associated with an altered S/G ratio in transgenic Populus lines. PtrLAC16 was localized exclusively in the cell walls of stem vascular tissue, and a reduction in PtrLAC16 expression led to a significant decrease in lignin content and altered cell wall structure. There was a direct correlation between the inhibition of PtrLAC16 expression and structural changes in the stem cell wall of Populus. This study provides direct evidence that PtrLAC16 plays a key role in the polymerization of lignin monomers, especially for sinapyl lignin, and affects the formation of xylem cell walls in Populus.
Asunto(s)
Biocatálisis , Pared Celular/enzimología , Lacasa/metabolismo , Lignina/metabolismo , Proteínas de Plantas/metabolismo , Polimerizacion , Populus/enzimología , Xilema/enzimología , Regulación de la Expresión Génica de las Plantas , Cinética , Lacasa/aislamiento & purificación , Especificidad de Órganos , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/aislamiento & purificación , Haz Vascular de Plantas/metabolismo , Plantas Modificadas Genéticamente , Populus/genética , Transporte de Proteínas , Espectrometría Raman , Fracciones Subcelulares/metabolismo , Nicotiana , Xilema/ultraestructuraRESUMEN
Cucurbit phloem is complex, with large sieve tubes on both sides of the xylem (bicollateral phloem), and extrafascicular elements that form an intricate web linking the rest of the vasculature. Little is known of the physical interconnections between these networks or their functional specialization, largely because the extrafascicular phloem strands branch and turn at irregular angles. Here, export in the phloem from specific regions of the lamina of cucumber (Cucumis sativus L.) was mapped using carboxyfluorescein and 14 C as mobile tracers. We also mapped vascular architecture by conventional microscopy and X-ray computed tomography using optimized whole-tissue staining procedures. Differential gene expression in the internal (IP) and external phloem (EP) was analyzed by laser-capture microdissection followed by RNA-sequencing. The vascular bundles of the lamina form a nexus at the petiole junction, emerging in a predictable pattern, each bundle conducting photoassimilate from a specific region of the blade. The vascular bundles of the stem interconnect at the node, facilitating lateral transport around the stem. Elements of the extrafascicular phloem traverse the stem and petiole obliquely, joining the IP and EP of adjacent bundles. Using pairwise comparisons and weighted gene coexpression network analysis, we found differences in gene expression patterns between the petiole and stem and between IP and EP, and we identified hub genes of tissue-specific modules. Genes related to transport were expressed primarily in the EP while those involved in cell differentiation and development as well as amino acid transport and metabolism were expressed mainly in the IP.
Asunto(s)
Cucumis sativus/ultraestructura , Cucumis sativus/genética , Cucumis sativus/metabolismo , Floema/genética , Floema/metabolismo , Floema/ultraestructura , Brotes de la Planta/genética , Brotes de la Planta/metabolismo , Brotes de la Planta/ultraestructura , Xilema/genética , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
KEY MESSAGE: Resistance conferred by the Cre8 locus of wheat prevents cereal cyst nematode feeding sites from reaching and invading root metaxylem vessels. Cyst nematodes develop syncytial feeding sites within plant roots. The success of these sites is affected by host plant resistance. In wheat (Triticum aestivum L.), 'Cre' loci affect resistance against the cereal cyst nematode (CCN) Heterodera avenae. To investigate how one of these loci (Cre8, on chromosome 6B) confers resistance, CCN-infected root tissue from susceptible (-Cre8) and resistant (+Cre8) wheat plants was examined using confocal microscopy and laser ablation tomography. Confocal analysis of transverse sections showed that feeding sites in the roots of -Cre8 plants were always adjacent to metaxylem vessels, contained many intricate 'web-like' cell walls, and sometimes 'invaded' metaxylem vessels. In contrast, feeding sites in the roots of +Cre8 plants were usually not directly adjacent to metaxylem vessels, had few inner cell walls and did not 'invade' metaxylem vessels. Models based on data from laser ablation tomography confirmed these observations. Confocal analysis of longitudinal sections revealed that CCN-induced xylem modification that had previously been reported for susceptible (-Cre8) wheat plants is less extreme in resistant (+Cre8) plants. Application of a lignin-specific stain revealed that secondary thickening around xylem vessels in CCN-infected roots was greater in +Cre8 plants than in -Cre8 plants. Collectively, these results indicate that Cre8 resistance in wheat acts by preventing cyst nematode feeding sites from reaching and invading root metaxylem vessels.
Asunto(s)
Resistencia a la Enfermedad/genética , Enfermedades de las Plantas/parasitología , Proteínas de Plantas/metabolismo , Triticum/parasitología , Tylenchida/fisiología , Animales , Pared Celular/parasitología , Pared Celular/ultraestructura , Susceptibilidad a Enfermedades , Sitios Genéticos , Imagenología Tridimensional , Enfermedades de las Plantas/prevención & control , Proteínas de Plantas/genética , Raíces de Plantas/genética , Raíces de Plantas/parasitología , Raíces de Plantas/ultraestructura , Triticum/genética , Triticum/ultraestructura , Xilema/genética , Xilema/parasitología , Xilema/ultraestructuraRESUMEN
Understanding tree physiological responses to fire is needed to accurately model post-fire carbon processes and inform management decisions. Given trees can die immediately or at extended time periods after fire, we combined two experiments to assess the short- (one-day) and long-term (21-months) fire effects on Pinus ponderosa sapling water transport. Native percentage loss of conductivity (nPLC), vulnerability to cavitation and xylem anatomy were assessed in unburned and burned saplings at lethal and non-lethal fire intensities. Fire did not cause any impact on nPLC and xylem cell wall structure in either experiment. However, surviving saplings evaluated 21-months post-fire were more vulnerable to cavitation. Our anatomical analysis in the long-term experiment showed that new xylem growth adjacent to fire scars had irregular-shaped tracheids and many parenchyma cells. Given conduit cell wall deformation was not observed in the long-term experiment, we suggest that the irregularity of newly grown xylem cells nearby fire wounds may be responsible for decreasing resistance to embolism in burned plants. Our findings suggest that hydraulic failure is not the main short-term physiological driver of mortality for Pinus ponderosa saplings. However, the decrease in embolism resistance in fire-wounded saplings could contribute to sapling mortality in the years following fire.
Asunto(s)
Incendios , Pinus ponderosa/fisiología , Tallos de la Planta/fisiología , Pinus ponderosa/metabolismo , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Plantones/fisiología , Agua/metabolismo , Xilema/metabolismo , Xilema/fisiología , Xilema/ultraestructuraRESUMEN
Lipids have been observed attached to lumen-facing surfaces of mature xylem conduits of several plant species, but there has been little research on their functions or effects on water transport, and only one lipidomic study of the xylem apoplast. Therefore, we conducted lipidomic analyses of xylem sap from woody stems of seven plants representing six major angiosperm clades, including basal magnoliids, monocots and eudicots, to characterize and quantify phospholipids, galactolipids and sulfolipids in sap using mass spectrometry. Locations of lipids in vessels of Laurus nobilis were imaged using transmission electron microscopy and confocal microscopy. Xylem sap contained the galactolipids di- and monogalactosyldiacylglycerol, as well as all common plant phospholipids, but only traces of sulfolipids, with total lipid concentrations in extracted sap ranging from 0.18 to 0.63 nmol ml-1 across all seven species. Contamination of extracted sap from lipids in cut living cells was found to be negligible. Lipid composition of sap was compared with wood in two species and was largely similar, suggesting that sap lipids, including galactolipids, originate from cell content of living vessels. Seasonal changes in lipid composition of sap were observed for one species. Lipid layers coated all lumen-facing vessel surfaces of L. nobilis, and lipids were highly concentrated in inter-vessel pits. The findings suggest that apoplastic, amphiphilic xylem lipids are a universal feature of angiosperms. The findings require a reinterpretation of the cohesion-tension theory of water transport to account for the effects of apoplastic lipids on dynamic surface tension and hydraulic conductance in xylem.
Asunto(s)
Lípidos/análisis , Magnoliopsida/química , Xilema/química , Galactolípidos/análisis , Galactolípidos/metabolismo , Lipidómica , Magnoliopsida/genética , Magnoliopsida/metabolismo , Microscopía Confocal , Microscopía Electrónica de Transmisión , Fosfolípidos/análisis , Fosfolípidos/metabolismo , Filogenia , Estaciones del Año , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
Lignin causes lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Engineered low-lignin plants have reduced recalcitrance but often exhibit yield penalties, offsetting their gains in fermentable sugar yield. Here, CRISPR/Cas9-generated CCR2(-/*) line 12 poplars have one knockout CCR2 allele while the other contains a 3-bp deletion, resulting in a 114I115A-to-114T conversion in the corresponding protein. Despite having 10% less lignin, CCR2(-/*) line 12 grows normally. On a plant basis, the saccharification efficiency of CCR2(-/*) line 12 is increased by 25-41%, depending on the pretreatment. Analysis of monoallelic CCR2 knockout lines shows that the reduced lignin amount in CCR2(-/*) line 12 is due to the combination of a null and the specific haploinsufficient CCR2 allele. Analysis of another CCR2(-/*) line shows that depending on the specific CCR2 amino-acid change, lignin amount and growth can be affected to different extents. Our findings open up new possibilities for stably fine-tuning residual gene function in planta.
Asunto(s)
Aldehído Oxidorreductasas/genética , Lignina/metabolismo , Populus/genética , Populus/metabolismo , Aldehído Oxidorreductasas/metabolismo , Alelos , Técnicas de Inactivación de Genes , Haploinsuficiencia , Lignina/genética , Mutación , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Populus/crecimiento & desarrollo , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
Pit membranes between xylem vessels play a major role in angiosperm water transport. Yet, their three-dimensional (3D) structure as fibrous porous media remains unknown, largely due to technical challenges and sample preparation artefacts. Here, we applied a modelling approach based on thickness measurements of fresh and fully shrunken pit membranes of seven species. Pore constrictions were also investigated visually by perfusing fresh material with colloidal gold particles of known sizes. Based on a shrinkage model, fresh pit membranes showed tiny pore constrictions of ca. 20 nm, but a very high porosity (i.e. pore volume fraction) of on average 0.81. Perfusion experiments showed similar pore constrictions in fresh samples, well below 50 nm based on transmission electron microscopy. Drying caused a 50% shrinkage of pit membranes, resulting in much smaller pore constrictions. These findings suggest that pit membranes represent a mesoporous medium, with the pore space characterized by multiple constrictions. Constrictions are much smaller than previously assumed, but the pore volume is large and highly interconnected. Pores do not form highly tortuous, bent, or zigzagging pathways. These insights provide a novel view on pit membranes, which is essential to develop a mechanistic, 3D understanding of air-seeding through this porous medium.
Asunto(s)
Magnoliopsida/ultraestructura , Xilema/ultraestructura , Acer/química , Transporte Biológico , Cinnamomum camphora/química , Constricción , Corylus/química , Fagus/química , Oro Coloide/química , Liriodendron/química , Microscopía Electrónica de Transmisión , Persea/química , Populus/química , Porosidad , Agua/fisiologíaRESUMEN
An in vitro induction system for xylem vessel formation is a useful tool for visualizing the differentiation of xylem vessel cells. A procedure for inducing xylem vessel cell differentiation in hypocotyls of Arabidopsis thaliana is described here. Metaxylem vessel elements form ectopically in excised hypocotyl tissue following treatment with bikinin. This enables high-resolution imaging of living metaxylem vessel cells. The wide range of resources available for Arabidopsis allows for the visualization of diverse cellular structures, including microtubules and secondary cell walls, in different genetic backgrounds. Use of this system will contribute to the further understanding of the processes by which xylem vessel elements form.
Asunto(s)
Arabidopsis/ultraestructura , Hipocótilo/ultraestructura , Microscopía Fluorescente/métodos , Microscopía de Interferencia/métodos , Xilema/ultraestructura , Arabidopsis/citología , Diferenciación Celular , Pared Celular/ultraestructura , Hipocótilo/citología , Microscopía Confocal/métodos , Microtúbulos , Imagen Óptica/métodos , Coloración y Etiquetado/métodos , Xilema/citologíaRESUMEN
The tracheary system of plant leaves is composed of a cellulose skeleton with diverse hierarchical structures. It is built of polygonally bent helical microfilaments of cellulose-based nanostructures coated by different layers, which provide them high compression resistance, elasticity, and roughness. Their function includes the transport of water and nutrients from the roots to the leaves. Unveiling details about local interactions of tracheary elements with surrounding material, which varies between plants due to adaptation to different environments, is crucial for understanding ascending fluid transport and for tracheary mechanical strength relevant to potential applications. Here we show that plant tracheary microfilaments, collected from Agapanthus africanus and Ornithogalum thyrsoides leaves, have different surface morphologies, revealed by nematic liquid crystal droplets. This results in diverse interactions among microfilaments and with the environment; the differences translate to diverse mechanical properties of entangled microfilaments and their potential applications. The presented study also introduces routes for accurate characterization of plants' microfilaments.
Asunto(s)
Citoesqueleto de Actina/ultraestructura , Plantas/ultraestructura , Citoesqueleto de Actina/fisiología , Amaryllidaceae/ultraestructura , Fenómenos Biomecánicos , Nanoestructuras/ultraestructura , Ornithogalum/ultraestructura , Hojas de la Planta/ultraestructura , Xilema/ultraestructuraRESUMEN
Wood fibers form thick secondary cell wall (SCW) in xylem tissues to give mechanical support to trees. NAC SECONDARY WALL THICKENING PROMOTING FACTOR3/SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN 1 (NST3/SND1) and NST1 were identified as master regulators of SCW formation in xylem fiber cells in the model plant Arabidopsis thaliana. In Populus species, four NST/SND orthologs have been conserved and coordinately control SCW formation in wood fibers and phloem fibers. However, it remains to be elucidated whether SCW formation in other xylem cells, such as ray parenchyma cells and vessel elements, is regulated by NST/SND orthologs in poplar. We knocked out all NST/SND genes in hybrid aspen using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) system and investigated the detailed histological appearance of stem tissues in the knockout mutants. Observation by light microscopy and transmission electron microscopy showed that SCW was severely suppressed in wood fibers, phloem fibers and xylem ray parenchyma cells in the knockout mutants. Although almost all wood fibers lacked SCW, some fiber cells formed thick cell walls. The irregularly cell wall-forming fibers retained primary wall and SCW, and were mainly located in the vicinity of vessel elements. Field emission-scanning electron microscope observation showed that there were no apparent differences in the structural features of pits such as the shape and size between irregularly SCW-forming wood fibers in the knockout mutants and normal wood fibers in wild-type. Cell wall components such as cellulose, hemicellulose and lignin were deposited in the cell wall of irregularly SCW-forming wood fibers in quadruple mutants. Our results indicate that four NST/SND orthologs are master switches for SCW formation in wood fibers, xylem ray parenchyma cells and phloem fibers in poplar, while SCW is still formed in limited wood fibers, which are located at the region adjacent to vessel elements in the knockout mutants.
Asunto(s)
Proteínas de Plantas/metabolismo , Populus/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Pared Celular/metabolismo , Pared Celular/ultraestructura , Celulosa/metabolismo , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Lignina/metabolismo , Floema/genética , Floema/fisiología , Floema/ultraestructura , Proteínas de Plantas/genética , Polisacáridos/metabolismo , Populus/fisiología , Populus/ultraestructura , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Madera/genética , Madera/fisiología , Madera/ultraestructura , Xilema/genética , Xilema/fisiología , Xilema/ultraestructuraRESUMEN
The Aurora kinases are serine/threonine kinases with conserved functions in mitotic cell division in eukaryotes. In Arabidopsis, Aurora kinases play important roles in primary meristem maintenance, but their functions in vascular development are still elusive. We report a dominant xdi-d mutant showing the xylem development inhibition (XDI) phenotype. Gene identification and transgenic overexpression experiments indicated that the activation of the Arabidopsis Aurora 2 (AtAUR2) gene is responsible for the XDI phenotype. In contrast, the aur1-2 aur2-2 double mutant plants showed enhanced differentiation of phloem and xylem cells, indicating that the Aurora kinases negatively affect xylem differentiation. The transcript levels of key regulatory genes in vascular cell differentiation, i.e. ALTERED PHLOEM DEVELOPMENT (APL), VASCULAR-RELATED NAC-DOMAIN 6 (VND6) and VND7, were higher in the aur1-2 aur2-2 double mutant and lower in xdi-d mutants compared with the wild-type plants, further supporting the functions of α-Aurora kinases in vascular development. Gene mutagenesis and transgenic studies showed that protein phosphorylation and substrate binding, but not protein dimerization and ubiquitination, are critical for the biological function of AtAUR2. These results indicate that α-Aurora kinases play key roles in vascular cell differentiation in Arabidopsis.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimología , Arabidopsis/crecimiento & desarrollo , Aurora Quinasas/metabolismo , Haz Vascular de Plantas/crecimiento & desarrollo , Proteínas Serina-Treonina Quinasas/metabolismo , Arabidopsis/genética , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Genes Reguladores , Prueba de Complementación Genética , Mutación/genética , Fenotipo , Floema/crecimiento & desarrollo , Floema/metabolismo , Xilema/crecimiento & desarrollo , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
The phloem of the Cucurbitaceae has long been a subject of interest due to its complex nature and the economic importance of the family. As in a limited number of other families, cucurbit phloem is bicollateral, i.e. with sieve tubes on both sides of the xylem. To date little is known about the specialized functions of the internal phloem (IP) and external phloem (EP). Here, a combination of microscopy, fluorescent dye transport analysis, micro-computed tomography, laser capture microdissection and RNA-sequencing (RNA-Seq) were used to study the functions of IP and EP in the vascular bundles (VBs) of cucumber fruit. There is one type of VB in the peduncle, but four in the fruit: peripheral (PeVB), main (MVB), carpel (CVB) and placental (PlVB). The VBs are bicollateral, except for the CVB and PlVB. Phloem mobile tracers and 14 C applied to leaves are transported primarily in the EP, and to a lesser extent in the IP. RNA-Seq data indicate preferential gene transcription in the IP related to differentiation/development, hormone transport, RNA or protein modification/processing/transport, and nitrogen compound metabolism and transport. The EP preferentially expresses genes for stimulus/stress, defense, ion transport and secondary metabolite biosynthesis. The MVB phloem is preferentially involved in photoassimilate transport, unloading and long-distance signaling, while the PeVB plays a more substantial role in morphogenesis and/or development and defense response. CVB and PlVB transcripts are biased toward development of reproductive organs. These findings provide an integrated view of the differentiated structure and function of the vascular tissue in cucumber fruit.
Asunto(s)
Cucumis sativus/metabolismo , Frutas/metabolismo , Floema/metabolismo , Cucumis sativus/crecimiento & desarrollo , Cucumis sativus/ultraestructura , Frutas/crecimiento & desarrollo , Frutas/ultraestructura , Perfilación de la Expresión Génica , Microscopía Confocal , Floema/crecimiento & desarrollo , Floema/ultraestructura , Tallos de la Planta/crecimiento & desarrollo , Tallos de la Planta/metabolismo , Tallos de la Planta/ultraestructura , Microtomografía por Rayos X , Xilema/crecimiento & desarrollo , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
Lycopsids are a minor component of current terrestrial herbaceous floras. However, lycopsid fossil diversity shows a great diversity and disparity including heterosporous woody plants, e.g. the giant isoetaleans that populated the extensive Pennsylvanian wetlands. The earliest known isoetaleans come from late Devonian localities from China. Here, we describe Lilingostrobus chaloneri gen. et sp. nov., a new isoetalean lycopsid from the Upper Devonian (Famennian) Xikuangshan Formation of China (Hunan Province, South China), which adds to the already impressive diversity of the Devonian lycopsids from China. Lilingostrobus shows an unusual combination of characters. This new plant is pseudoherbaceous, with a possible tufted habit, and consists of narrow axes with rare isotomies. The stem includes small quantities of secondary xylem. Each fertile axis bears one terminal strobilus comprising sporophylls ending in a very long upturned lamina. Microspores and putative megaspores have been found, but whether the plant has mono- or bisporangiate strobili is unknown. Importantly, our cladistic analysis identifies Lilingostrobus as a direct precursor of Isoetales, which provides new insights into the early evolution of lycopsids.
Asunto(s)
Lycopodiaceae/anatomía & histología , Filogenia , Tallos de la Planta/anatomía & histología , Xilema/anatomía & histología , Evolución Biológica , China , Extinción Biológica , Fósiles/historia , Historia Antigua , Lycopodiaceae/clasificación , Lycopodiaceae/fisiología , Tallos de la Planta/clasificación , Tallos de la Planta/fisiología , Humedales , Xilema/fisiología , Xilema/ultraestructuraRESUMEN
The popularity of X-ray based imaging methods has continued to increase in research domains. In wood research, X-ray micro-computed tomography (XµCT) is useful for structural studies examining the three-dimensional and complex xylem tissue of trees qualitatively and quantitatively. In this study, XµCT made it possible to visualize and quantify the spatial xylem organization of the angiosperm species Fraxinus excelsior L. on the microscopic level. Through image analysis, it was possible to determine morphological characteristics of the cellular axial tissue (vessel elements, fibers, and axial parenchyma cells) three-dimensionally. X-ray imaging at high resolutions provides very distinct visual insight into the xylem structure. Numerical analyses performed through semi-automatic procedures made it possible to quickly quantify cell characteristics (length, diameter, and volume of cells). Use of various spatial resolutions (0.87-5⯵m) revealed boundaries users should be aware of. Nevertheless, our findings, both qualitative and quantitative, demonstrate XµCT to be a valuable tool for studying the spatial cell morphology of F. excelsior.
Asunto(s)
Fraxinus/ultraestructura , Xilema/ultraestructura , Imagenología Tridimensional , Microtomografía por Rayos XRESUMEN
Ophiognomonia clavigignenti-juglandacearum endangers the survival of butternut (Juglans cinerea) throughout its native range. While screening for disease resistance, we found that artificial inoculations of 48 butternut seedlings with O. clavigignenti-juglandacearum induced the expression of external symptoms, but only after a period of dormancy. Before dormancy, compartmentalized tissues such as necrophylactic periderms (NPs) and xylem reaction zones (RZs) contributed to limiting pathogen invasion. Phenols were regularly detected in RZs, often in continuity with NPs during wound closure, and confocal microscopy revealed their presence in parenchyma cells, vessel plugs and cell walls. Vessels were blocked with tyloses and gels, particularly those present in RZs. Suberin was also detected in cells formed over the affected xylem by the callus at the inoculation point, in a few tylosis walls, and in longitudinal tubes that formed near NPs. Following dormancy, in all inoculated seedlings but one, defensive barriers were breached by O. clavigignenti-juglandacearum and then additional ones were produced in response to this new invasion. The results of this histopathological study indicate that trees inoculated in selection programs to test butternut canker resistance should go through at least one period of dormancy and that asymptomatic individuals should be dissected to better assess how they defend themselves against O. clavigignenti-juglandacearum.
Asunto(s)
Ascomicetos/fisiología , Resistencia a la Enfermedad , Juglans/inmunología , Enfermedades de las Plantas/inmunología , Pared Celular/ultraestructura , Celulosa/análogos & derivados , Celulosa/metabolismo , Juglans/metabolismo , Juglans/microbiología , Juglans/ultraestructura , Fenoles/metabolismo , Enfermedades de las Plantas/microbiología , Latencia en las Plantas , Plantones/inmunología , Plantones/microbiología , Plantones/ultraestructura , Xilema/inmunología , Xilema/microbiología , Xilema/ultraestructuraRESUMEN
Some temperate tree species mitigate the negative impacts of frost-induced xylem cavitation by restoring impaired hydraulic function via positive pressures, and may therefore be more resistant to frost fatigue (the phenomenon that post-freezing xylem becomes more susceptible to hydraulic dysfunction) than nonpressure-generating species. We test this hypothesis and investigate underlying anatomical/physiological mechanisms. Using a common garden experiment, we studied key hydraulic traits and detailed xylem anatomical characteristics of 18 sympatric tree species. These species belong to three functional groups, that is, one generating both root and stem pressures (RSP), one generating only root pressure (RP), and one unable to generate such pressures (NP). The three functional groups diverged substantially in hydraulic efficiency, resistance to drought-induced cavitation, and frost fatigue resistance. Most notably, RSP and RP were more resistant to frost fatigue than NP, but this was at the cost of reduced hydraulic conductivity for RSP and reduced resistance to drought-induced cavitation for RP. Our results show that, in environments with strong frost stress: these groups diverge in hydraulic functioning following multiple trade-offs between hydraulic efficiency, resistance to drought and resistance to frost fatigue; and how differences in anatomical characteristics drive such divergence across species.
Asunto(s)
Congelación , Raíces de Plantas/fisiología , Tallos de la Planta/fisiología , Presión , Árboles/fisiología , Agua/fisiología , Análisis de Componente Principal , Carácter Cuantitativo Heredable , Especificidad de la Especie , Madera/fisiología , Xilema/fisiología , Xilema/ultraestructuraRESUMEN
The phytohormone ethylene impacts secondary stem growth in plants by stimulating cambial activity, xylem development and fiber over vessel formation. We report the effect of ethylene on secondary cell wall formation and the molecular connection between ethylene signaling and wood formation. We applied exogenous ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC) to wild-type and ethylene-insensitive hybrid aspen trees (Populus tremula × tremuloides) and studied secondary cell wall anatomy, chemistry and ultrastructure. We furthermore analyzed the transcriptome (RNA Seq) after ACC application to wild-type and ethylene-insensitive trees. We demonstrate that ACC and ethylene induce gelatinous layers (G-layers) and alter the fiber cell wall cellulose microfibril angle. G-layers are tertiary wall layers rich in cellulose, typically found in tension wood of aspen trees. A vast majority of transcripts affected by ACC are downstream of ethylene perception and include a large number of transcription factors (TFs). Motif-analyses reveal potential connections between ethylene TFs (Ethylene Response Factors (ERFs), ETHYLENE INSENSITIVE 3/ETHYLENE INSENSITIVE3-LIKE1 (EIN3/EIL1)) and wood formation. G-layer formation upon ethylene application suggests that the increase in ethylene biosynthesis observed during tension wood formation is important for its formation. Ethylene-regulated TFs of the ERF and EIN3/EIL1 type could transmit the ethylene signal.
Asunto(s)
Etilenos/metabolismo , Hibridación Genética , Populus/metabolismo , Transducción de Señal , Madera/metabolismo , Aminoácidos Cíclicos/farmacología , Pared Celular/efectos de los fármacos , Pared Celular/metabolismo , Pared Celular/ultraestructura , Celulosa/metabolismo , Simulación por Computador , Genes de Plantas , Populus/genética , Populus/ultraestructura , Análisis de Componente Principal , Regiones Promotoras Genéticas/genética , Espectroscopía Infrarroja por Transformada de Fourier , Agua/farmacología , Madera/efectos de los fármacos , Madera/crecimiento & desarrollo , Madera/ultraestructura , Xilema/efectos de los fármacos , Xilema/metabolismo , Xilema/ultraestructuraRESUMEN
BACKGROUND: Vessel-associated cells (VACs) are highly specialized, living parenchyma cells that are in direct contact with water-conducting, dead vessels. The contact may be sparse or in large tight groups of parenchyma that completely surrounds vessels. VACs differ from vessel distant parenchyma in physiology, anatomy, and function and have half-bordered pits at the vessel-parenchyma juncture. The distinct anatomy of VACs is related to the exchange of substances to and from the water-transport system, with the cells long thought to be involved in water transport in woody angiosperms, but where direct experimental evidence is lacking. SCOPE: This review focuses on our current knowledge of VACs regarding anatomy and function, including hydraulic capacitance, storage of nonstructural carbohydrates, symplastic and apoplastic interactions, defense against pathogens and frost, osmoregulation, and the novel hypothesis of surfactant production. Based on microscopy, we visually represent how VACs vary in dimensions and general appearance between species, with special attention to the protoplast, amorphous layer, and the vessel-parenchyma pit membrane. CONCLUSIONS: An understanding of the relationship between VACs and vessels is crucial to tackling questions related to how water is transported over long distances in xylem, as well as defense against pathogens. New avenues of research show how parenchyma-vessel contact is related to vessel diameter and a new hypothesis may explain how surfactants arising from VAC can allow water to travel under negative pressure. We also reinforce the message of connectivity between VAC and other cells between xylem and phloem.
Asunto(s)
Magnoliopsida/citología , Xilema/citología , Magnoliopsida/fisiología , Magnoliopsida/ultraestructura , Agua/metabolismo , Xilema/fisiología , Xilema/ultraestructuraRESUMEN
PREMISE OF THE STUDY: Xylem sap in angiosperms moves under negative pressure in conduits and cell wall pores that are nanometers to micrometers in diameter, so sap is always very close to surfaces. Surfaces matter for water transport because hydrophobic ones favor nucleation of bubbles, and surface chemistry can have strong effects on flow. Vessel walls contain cellulose, hemicellulose, lignin, pectins, proteins, and possibly lipids, but what is the nature of the inner, lumen-facing surface that is in contact with sap? METHODS: Vessel lumen surfaces of five angiosperms from different lineages were examined via transmission electron microscopy and confocal and fluorescence microscopy, using fluorophores and autofluorescence to detect cell wall components. Elemental composition was studied by energy-dispersive X-ray spectroscopy, and treatments with phospholipase C (PLC) were used to test for phospholipids. KEY RESULTS: Vessel surfaces consisted mainly of lignin, with strong cellulose signals confined to pit membranes. Proteins were found mainly in inter-vessel pits and pectins only on outer rims of pit membranes and in vessel-parenchyma pits. Continuous layers of lipids were detected on most vessel surfaces and on most pit membranes and were shown by PLC treatment to consist at least partly of phospholipids. CONCLUSIONS: Vessel surfaces appear to be wettable because lignin is not strongly hydrophobic and a coating with amphiphilic lipids would render any surface hydrophilic. New questions arise about these lipids and their possible origins from living xylem cells, especially about their effects on surface tension, surface bubble nucleation, and pit membrane function.
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Magnoliopsida/ultraestructura , Xilema/ultraestructura , Pared Celular/fisiología , Pared Celular/ultraestructura , Lignina/metabolismo , Magnoliopsida/fisiología , Lípidos de la Membrana/metabolismo , Microscopía Confocal , Microscopía Electrónica de Transmisión , Microscopía Fluorescente , Propiedades de Superficie , Agua/metabolismo , Xilema/fisiologíaRESUMEN
Plants close their stomata during drought to avoid excessive water loss, but species differ in respect to the drought severity at which stomata close. The stomatal closure point is related to xylem anatomy and vulnerability to embolism, but it also has implications for phloem transport and possibly phloem anatomy to allow sugar transport at low water potentials. Desiccation-tolerant plants that close their stomata at severe drought should have smaller xylem conduits and/or fewer and smaller interconduit pits to reduce vulnerability to embolism but more phloem tissue and larger phloem conduits compared with plants that avoid desiccation. These anatomical differences could be expected to increase in response to long-term reduction in precipitation. To test these hypotheses, we used tridimensional synchroton X-ray microtomograph and light microscope imaging of combined xylem and phloem tissues of 2 coniferous species: one-seed juniper (Juniperus monosperma) and piñon pine (Pinus edulis) subjected to precipitation manipulation treatments. These species show different xylem vulnerability to embolism, contrasting desiccation tolerance, and stomatal closure points. Our results support the hypothesis that desiccation tolerant plants require higher phloem transport capacity than desiccation avoiding plants, but this can be gained through various anatomical adaptations in addition to changing conduit or tissue size.