RESUMO
Studies have suggested a possible link between breast cancer pathogenesis and human papillomavirus (HPV) infection. This study in Iraq used in situ hybridization to detect the frequency and genotyping of HPV in tissue specimens from 129 patients diagnosed with malignant breast cancer, 24 with benign breast tumours and 20 healthy controls. In the breast cancer group, cocktail HPV genotypes were detected in 60 (46.5%) archived tissue blocks. Of these, genotypes 16 (55.5%), 18 (58.4%), 31 (65.0%) and 33 (26.6%) were detected. Mixed HPV genotypes 16 + 18, 16 + 18 + 31, 16 + 18 + 33, 18 + 33, 16 + 31 and 18 + 31 were found in 5.0%, 25.0%, 8.3%, 7.7%, 10.0% and 13.3% of cancer cases respectively. Only 3 benign breast tumour tissues (12.5%) and none of the healthy breast tissue specimens were HPV-DNA-positive. The detection of high-oncogenic HPV genotypes in patients with breast cancer supports the hypothesis of an etiologic role for the virus in breast cancer development.
Assuntos
Neoplasias da Mama/virologia , Papillomaviridae/genética , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Genótipo , Humanos , Hibridização In Situ , Iraque , Pessoa de Meia-Idade , Papillomaviridae/isolamento & purificação , Estudos RetrospectivosRESUMO
We studied the DNA content, DNA index and cell cycle parameters that are reliable markers for assessing the proliferative activity and aggressiveness of malignancies. Cytometric DNA analysis was performed on formalin-fixed paraffin embedded sections from 36 Iraqi patients with oral squamous cell carcinoma. The results showed that 20 of 36 cases (55.5%) were diploid, while 15 cases (41.7%) were aneuploid. Significantly higher S-phase fractions and higher DNA indices characterized aneuploid tumours. Nuclear DNA analysis as part of the evaluation of oral squamous cell carcinoma will influence the selection of appropriate treatment strategies.
Assuntos
Carcinoma de Células Escamosas/patologia , DNA de Neoplasias , Citometria de Fluxo/métodos , Neoplasias Bucais/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Aneuploidia , Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/cirurgia , Estudos de Casos e Controles , DNA de Neoplasias/análise , DNA de Neoplasias/genética , Diploide , Feminino , Citometria de Fluxo/normas , Humanos , Iraque/epidemiologia , Modelos Lineares , Metástase Linfática/patologia , Masculino , Pessoa de Meia-Idade , Índice Mitótico , Neoplasias Bucais/epidemiologia , Neoplasias Bucais/cirurgia , Estadiamento de Neoplasias , Seleção de Pacientes , Prognóstico , Fase S/genéticaRESUMO
A cohort of 77 women referred for routine screening or investigation of Pap test abnormality underwent colposcopic examination. Pap-stained liquid-based preparations were diagnosed and categorized according to the Bethesda system. Residual material on the sampling device was used to detect high-risk oncogenic human papillomavirus DNA. Although the colposcopic failure rate was higher than that of cytology, no lesion was missed when both methods were used together. High-risk types were recorded in 24% of patients with atypical squamous cells of undetermined significance, 45% with low-grade squamous intraepithelial lesions and 79% with high-grade squamous intraepithelial lesions--indicating that the efficacy of cytological screening can be improved by papillomavirus detection.
Assuntos
Colposcopia/normas , Condiloma Acuminado/complicações , Condiloma Acuminado/diagnóstico , Programas de Rastreamento/métodos , Doenças do Colo do Útero/complicações , Doenças do Colo do Útero/diagnóstico , Displasia do Colo do Útero/complicações , Displasia do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/virologia , Esfregaço Vaginal/normas , Adulto , Idoso , Feminino , Humanos , Programas de Rastreamento/normas , Pessoa de Meia-Idade , Fatores de Risco , Sensibilidade e EspecificidadeRESUMO
This study estimated nuclear DNA ploidy and DNA proliferative indices (PI) in mammary ductal carcinomas from 120 Iraqi female patients. Of the examined specimens, 82.7% were aneuploid. DNA ploidy correlated significantly with histological grade and estrogen receptor content of the primary neoplasm. In aneuploid carcinomas, high PI showed a clearer association than aneuploidy with menopausal status and progesterone receptor content of the tumour. PI and percentage aneuploidy were higher in larger tumours; nodal status showed no association with these cytometric findings. Using PI, patients classified as having Auer aneuploid carcinomas can be divided into subsets with different tumour characteristics, thus improving the selection of those whose high risk, node-negative presentation makes them candidates for adjuvant systemic therapy.
Assuntos
Aneuploidia , Biomarcadores Tumorais/genética , Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/patologia , DNA de Neoplasias/genética , Índice Mitótico , Ploidias , Biópsia por Agulha , Neoplasias da Mama/química , Neoplasias da Mama/classificação , Neoplasias da Mama/terapia , Carcinoma Ductal de Mama/química , Carcinoma Ductal de Mama/classificação , Carcinoma Ductal de Mama/terapia , Feminino , Humanos , Iraque , Menopausa , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Seleção de Pacientes , Prognóstico , Estudos Prospectivos , Receptores de Estrogênio/análise , Receptores de Progesterona/análise , Fatores de RiscoAssuntos
Calcinose/diagnóstico , Litíase/diagnóstico , Pneumopatias/diagnóstico , Alvéolos Pulmonares , Adulto , Biópsia , Líquido da Lavagem Broncoalveolar/química , Líquido da Lavagem Broncoalveolar/citologia , Calcinose/epidemiologia , Calcinose/etiologia , Diagnóstico Diferencial , Progressão da Doença , Dispneia/etiologia , Feminino , Humanos , Iraque/epidemiologia , Litíase/epidemiologia , Litíase/etiologia , Pneumopatias/epidemiologia , Pneumopatias/etiologia , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios XRESUMO
Eighty-eight women presenting with locally advanced or metastatic breast cancer were treated with tamoxifen alone. Estrogen and progesterone receptors (ER and PR) were immunocytochemically analysed in mammary tumour cells obtained by fine needle sampling from 73 patients. Of the breast carcinomas, 34.2% were ER+/PR+ and 43.8% were ER-/PR-. The ER+ content increased with age in postmenopausal women. After tamoxifen treatment objective remission occurred in 39.7% of the women. The overall response rate was 53.3% in the ER+/PR- group and 73.1% in the ER+/PR+ group. However, the response elicited in a case of the ER-/PR- phenotype justified the randomized use of tamoxifen among patients in Iraq where the necessary requirements for hormone receptor assessment are almost unavailable.
