RESUMO
Oral inoculation of newborn mice with the MET strain of human rotavirus produced transient diarrhoeal disease. Light and scanning electron microscopy showed typical rotavirus-induced morphological lesions in the villous epithelium of the small intestine consisting of extensive cytoplasmic vacuolation, villous necrosis and atrophy. Virus recovered from intestinal suspensions of infected mice showed the typical electrophoretic profile of the genome of the inoculated strain. Rotavirus antibody appeared in infected mice 10 to 20 days after inoculation but not in controls or nursing dams. The availability of a small animal model for experimental infection with human rotaviruses should prove useful for virulence and protection studies.
Assuntos
Diarreia/microbiologia , Rotavirus/patogenicidade , Animais , Animais Recém-Nascidos , Diarreia/patologia , Humanos , Intestinos/microbiologia , Camundongos , Microscopia Eletrônica de Varredura , RNA de Cadeia Dupla/análise , RNA Viral/análise , Rotavirus/crescimento & desenvolvimento , Replicação ViralRESUMO
Three women with extrinsic oculo-muscular distrophy were studied. In two patients the symptoms were began at 25 and another one at 56 year-old. Non myogenic features were observed: in case one there was familial otosclerosis. This patient had impossibility to beget children. Her first menstruation was observed at 19 and the last at 40 year-old, like to case 2, which was the only to have electrocardiographic alterations. Romberg's signal and profound hyporeflexia was obtained in case 3, whose family had a lot of member with cataract. Biopsy of the non ocular muscles was made too, in spite of patient's symptoms had been concerning to ocular muscles only. All of non ocular muscles had myogenic features. In electromyogram examination of non ocular muscles the myogenic features were observed too. Therefore, we believe in diffuse myogenic process in spite of ocular manifestation had been the only patient's complaints.
Assuntos
Blefaroptose/genética , Distrofias Musculares/genética , Oftalmoplegia/genética , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Músculos/embriologia , Músculos/patologia , Distrofias Musculares/patologia , Nervo Oculomotor/patologiaRESUMO
A case of metachromatic leucodystrophy in a three years old female patient is reported. Histopathological examination revealed metachromatic inclusions within the cytoplasms of the neurons, which led the authors to comment on the physiopathogenesis of this disease, grouping it in category of the lipidoses.
