Cyanobacteria: A Promising Source of Antifungal Metabolites.

Cyanobacteria are a rich source of secondary metabolites, and they have received a great deal of attention due to their applicability in different industrial sectors. Some of these substances are known for their notorious ability to inhibit fungal growth. Such metabolites are very chemically and biologically diverse. They can belong to different chemical classes, including peptides, fatty acids, alkaloids, polyketides, and macrolides. Moreover, they can also target different cell components. Filamentous cyanobacteria have been the main source of these compounds. This review aims to identify the key features of these antifungal agents, as well as the sources from which they are obtained, their major targets, and the environmental factors involved when they are being produced. For the preparation of this work, a total of 642 documents dating from 1980 to 2022 were consulted, including patents, original research, review articles, and theses.

Anabaenopeptins: What We Know So Far.

Cyanobacteria are microorganisms with photosynthetic mechanisms capable of colonizing several distinct environments worldwide. They can produce a vast spectrum of bioactive compounds with different properties, resulting in an improved adaptative capacity. Their richness in secondary metabolites is related to their unique and diverse metabolic apparatus, such as Non-Ribosomal Peptide Synthetases (NRPSs). One important class of peptides produced by the non-ribosomal pathway is anabaenopeptins. These cyclic hexapeptides demonstrated inhibitory activity towards phosphatases and proteases, which could be related to their toxicity and adaptiveness against zooplankters and crustaceans. Thus, this review aims to identify key features related to anabaenopeptins, including the diversity of their structure, occurrence, the biosynthetic steps for their production, ecological roles, and biotechnological applications.

Impacts of Microcystins on Morphological and Physiological Parameters of Agricultural Plants: A Review.

Cyanobacteria are a group of photosynthetic prokaryotes that pose a great concern in the aquatic environments related to contamination and poisoning of wild life and humans. Some species of cyanobacteria produce potent toxins such as microcystins (MCs), which are extremely aggressive to several organisms, including animals and humans. In order to protect human health and prevent human exposure to this type of organisms and toxins, regulatory limits for MCs in drinking water have been established in most countries. In this regard, the World Health Organization (WHO) proposed 1 µg MCs/L as the highest acceptable concentration in drinking water. However, regulatory limits were not defined in waters used in other applications/activities, constituting a potential threat to the environment and to human health. Indeed, water contaminated with MCs or other cyanotoxins is recurrently used in agriculture and for crop and food production. Several deleterious effects of MCs including a decrease in growth, tissue necrosis, inhibition of photosynthesis and metabolic changes have been reported in plants leading to the impairment of crop productivity and economic loss. Studies have also revealed significant accumulation of MCs in edible tissues and plant organs, which raise concerns related to food safety. This work aims to systematize and analyze the information generated by previous scientific studies, namely on the phytotoxicity and the impact of MCs especially on growth, photosynthesis and productivity of agricultural plants. Morphological and physiological parameters of agronomic interest are overviewed in detail in this work, with the aim to evaluate the putative impact of MCs under field conditions. Finally, concentration-dependent effects are highlighted, as these can assist in future guidelines for irrigation waters and establish regulatory limits for MCs.

Current Knowledge on Microviridin from Cyanobacteria.

Cyanobacteria are a rich source of secondary metabolites with a vast biotechnological potential. These compounds have intrigued the scientific community due their uniqueness and diversity, which is guaranteed by a rich enzymatic apparatus. The ribosomally synthesized and post-translationally modified peptides (RiPPs) are among the most promising metabolite groups derived from cyanobacteria. They are interested in numerous biological and ecological processes, many of which are entirely unknown. Microviridins are among the most recognized class of ribosomal peptides formed by cyanobacteria. These oligopeptides are potent inhibitors of protease; thus, they can be used for drug development and the control of mosquitoes. They also play a key ecological role in the defense of cyanobacteria against microcrustaceans. The purpose of this review is to systematically identify the key characteristics of microviridins, including its chemical structure and biosynthesis, as well as its biotechnological and ecological significance.

Determination of Volatile Organic Compounds and Antibacterial Activity of the Amazonian Cyanobacterium Synechococcus sp. Strain GFB01.

Cyanobacteria exhibit great biotechnological potential due to their capacity to produce compounds with various applicability. Volatile organic compounds (VOCs) possess low molecular weight and high vapor pressure. Many volatiles produced by microorganisms have biotechnological potential, including antimicrobial activity. This study aimed to investigate the VOCs synthesized by cyanobacterium Synechococcus sp. strain GFB01, and the influence of nitrate and phosphate on its antibacterial potential. The strain was isolated from the surface of the freshwater lagoon Lagoa dos Índios, Amapá state, in Northern Brazil. After cultivation, the VOCs were extracted by a simultaneous distillation-extraction process, using a Likens-Nickerson apparatus (2 h), and then identified by GC-MS. The extracts did not display inhibitory activity against the Gram-positive bacteria tested by the disk-diffusion agar method. However, the anti-Salmonella property in both extracts (methanol and aqueous) was detected. The main VOCs identified were heptadecane (81.32%) and octadecyl acetate (11.71%). To the best of our knowledge, this is the first study of VOCs emitted by a cyanobacterium from the Amazon that reports the occurrence of 6-pentadecanol and octadecyl acetate in cyanobacteria.