Radiographic and computed tomographic characteristics of intraluminal tracheal adenoma in a cat.

A 13-year-old spayed female Persian cat presented with dyspnea and nasal discharge. Thoracic radiography revealed a dome-shaped soft-tissue opacity in the carina. Computed tomography confirmed a soft tissue-attenuating mass in the carina and the left and right proximal main bronchi that appeared to arise from the tracheal wall. Tracheoscopy revealed an intraluminal broad-based mass with multilobulated borders at the same location. Histopathological evaluation revealed a benign neoplastic process of the glandular epithelial lineage, which was considered an adenoma. Tracheal adenomas should be included in the differential diagnosis of tracheal masses.

Computed tomographic measurements of pancreatic thickness in clinically normal dogs.

Pancreatic thickness is an indicator for evaluating pancreatic diseases. The transverse and cross-sectional pancreatic thickness observed on computed tomography (CT) may differ. This study aimed to provide a normal reference range for pancreatic thickness on the transverse plane based on body weight (BW) and assess pancreatic thickness to aorta (P/Ao) ratio. In addition, we aimed to establish the normal short and long dimensions of the pancreas based on cross-sectional image through the long axis of the pancreas using multiplanar reconstruction (MPR). The short dimension to aorta (S/Ao) and long dimension to aorta (L/Ao) ratios were also established in clinically normal dogs. The pancreatic thickness was measured using CT results of 205 clinically normal dogs. The pancreatic thickness on the transverse plane and the short and long dimensions in the cross-sectional image of the pancreas were measured using MPR. The diameter of the Ao was measured on the transverse plane and the P/Ao, S/Ao, and L/Ao ratios were calculated. Our study showed that the mean normal pancreatic thicknesses (mean ± standard deviation [SD]) of the pancreatic body, left and right lobe in the transverse plane were 10.92 ± 2.54 mm, 8.92 ± 2.26 mm and 9.96 ± 2.24 mm, respectively. The P/Ao ratios of the pancreatic body, left and right lobes were 1.85 ± 0.33, 1.50 ± 0.27 and 1.68 ± 0.29, respectively. The mean short dimension (mean ± SD) in the cross-sectional image of the pancreatic body, left and right lobe were 8.98 ± 1.97 mm, 7.99 ± 1.89 mm and 8.76 ± 2.03 mm, respectively. In conclusion, pancreatic thickness increased with BW, while the P/Ao, S/Ao, and L/Ao ratios could be used regardless of BW.

Real-time gene delivery vector tracking in the endo-lysosomal pathway of live cells.

Using live-cell confocal microscopy and particle tracking technology, the simultaneous transport of intracellular vesicles of the endo-lysosomal pathway and nonviral polyethylenimine (PEI)/DNA nanocomplexes was investigated. Due to potential problems associated with the use of acid-sensitive probes in combination with a gene vector that is hypothesized to buffer the pH of intracellular vesicles, the biological location of PEI/DNA gene vectors was revealed by probing their trafficking in cells expressing fluorescent versions of either early endosome antigen 1, a protein that localizes to early endosomes, or Niemann Pick C1, a protein that localizes to late endosomes and lysosomes. Studies directly show that PEI/DNA nanoparticles are actively transported within both early and late endosomes, and display similar overall transport rates in each. Additionally, gene vector transfer between endosomes is observed. Over time post-transfection, gene vectors accumulate in late endosomes/lysosomes; however, real-time escape of vectors from membrane-bound vesicles is not observed.