Nitrofurantoin removal by the photo-Fenton process: degradation, mineralization, and biological inactivation.

Antibiotics may induce super-resistant bacteria if they are available in the environment. Therefore, the removal of aqueous nitrofurantoin (NFT), and more importantly, the removal of the remaining antimicrobial activity after treatment, by the photo-Fenton process, was herein studied. Degradation experiments were performed according to an experimental design (0.5% error; factors: concentrations of NFT, Fe3+, and H2O2). Degradation conditions were: 20 mg NFT L-1, 10 mg Fe3+ L-1, and 170 mg H2O2 L-1. Fixed parameters were: 100 mL of the NFT solution, pH 2.5, 15-min stirring, and 25.0 ± 0.5°C. The initial rate constant (k0) and the maximum oxidation capacity (MOC) of the system were 0.61 min-1 and 100%, respectively (R2 = 0.986). 97% of the NFT and 93% of the organic carbon initially present were removed. Five degradation products (DPs) were detected by HPLC-MS and their endpoints estimated by the ECOSAR (ECOlogical Structure-Activity Relationships) 2.0 software. NFT and its DPs presented no toxicity towards Lactuca sativa. The antimicrobial activity (Escherichia coli) of NFT and/or DPs was completely removed in 15 min. Structures were proposed for the detected DPs. In short, the tested advanced oxidation technology (AOP), besides being capable of removing and mineralizing aqueous NFT in a short time, 15 min, also rendered the treated water biologically inactive (no ecotoxicity, no antimicrobial activity).

Modified QuEChERS Method for Extracting Thiamethoxam and Imidacloprid from Stingless Bees: Development, Application, and Green Metrics.

In the present study, a method for the determination of residues of the neonicotinoid insecticides thiamethoxam and imidacloprid in the stingless bee Melipona scutellaris Latreille (1811) was optimized through a factorial design, tested using green metrics, and then applied to exposed bees. It combines the extraction with a modified quick, easy, cheap, effective, rugged, and safe method and the determination by liquid chromatography-tandem mass spectrometry analysis. Different parameters such as the mass of the sample, dispersive sorbents, and elution solvents were assessed. Method validation parameters were checked and include sensitivity, specificity, and linearity. The limit of quantification of 0.0025 µg g-1 was obtained for both insecticides, where accuracy was 94%-100% with satisfactory intraday and interday precisions (relative standard deviation <10%). The qualified method was applied to orally and topically exposed bee samples, and the results indicated that it is suitable for the determination and quantification of neonicotinoid pesticide residues in this species. Moreover, green analytical metrics like the National Environmental Methods Index, Eco Scale score, high-performance liquid chromatography with an environmental assessment tool (HPLC-EAT), waste generation, and amount of sample were compared with methods described in the literature involving neonicotinoid analysis in honeybees. As a result, the present study displayed the highest Eco Scale score and HPLC-EAT score and the second smallest amount of sample and waste generated. Thus, the method meets green analytical metrics more than other methods. In this sense, besides the application, the multicriteria decision analysis tool employed suggests that this is a good option as a green analytical method. Environ Toxicol Chem 2022;41:2365-2374. © 2022 SETAC.

Establishing simultaneous nitrification and denitrification under continuous aeration for the treatment of multi-electrolytes saline wastewater.

Simultaneous nitrification and denitrification (SND) was established under continuous aeration (6 mgO2 L-1) aiming at achieving a feasible and simple operational strategy for treating multi-electrolyte saline wastewaters. Two Structured Fixed-Bed Reactors (SFBR) were used to assess SND performance with (Saline Reactor, SR) and without (Control Reactor, CR) salinity interference. Salinity was gradually increased (from 1.7 to 9 atm) based on the composition of water supplied in arid regions of Brazil. At 1.7 atm, N-NH4+ oxidation and Total Nitrogen (TN) removal efficiencies of 95.9 ±â€¯2.8 and 65.76 ±â€¯7.5%, respectively, were obtained. At osmotic pressure (OP) of 9 atm, the system was severely affected by specific salt toxicity and OP. High chemical oxygen demand (COD) removal efficiency was achieved at all operational conditions (97.2 ±â€¯1.6 to 78.5 ±â€¯4.6%). Salinity did not affect microbial diversity, although it modified microbial structure. Halotolerant genera were identified (Prosthecobacter, Chlamydia, Microbacterium, and Paenibacillus).

The effect of cations (Na+, Mg2+, and Ca2+) on the activity and structure of nitrifying and denitrifying bacterial communities.

Wastewaters generated in regions with water scarcity usually have high alkalinity, hardness, and elevated osmotic pressure (OP). Those characteristics should be considered when using biological systems for wastewater treatment along with the salinity heterogeneity. The interaction of different salts in mixed electrolyte solutions may cause inhibition, antagonism, synergism, and stimulation effects on microbial communities. Little is known about those effects on microbial activity and community structure of nitrifying and denitrifying bacteria. In this work, factorial design was used to evaluate the effects of NaCl, MgCl2 and CaCl2 on nitrifying and denitrifying communities. Antagonistic relationships between all salts were observed and they had greater magnitude on the nitrifying community. Stimulus and synernism were more evident on the nitrifying and denitrifying experiments, respectively. For this reason, the highest nitrification and denitrification specific rates were 1.1 × 10-1 mgN-NH4+ gSSV-1 min-1 for condition 01 and 6.5 × 10-2 mgN-NO3- gSSV-1 min-1 for control condition, respectively. The toxicity of the salts followed the order of NaCl > MgCl2 > CaCl2 and the antagonism between MgCl2 and NaCl was the most significant. PCR/DGGE analyses showed that Mg2+ may be the element that expresses the least influence in the differentiation of microbial structure even though it significantly affects the activity of the autotrophic microorganisms. The same behavior was observed for Ca2+ on denitrifying microorganism. In addition, microbial diversity and richness was not negatively affected by different salinities. Genetic sequencing suggested that the genus Aeromonas, Alishewanella, Azospirillum, Pseudoalteromonas, and Thioalkalivibrio were outstanding on ammonium and nitrate removal under saline conditions. The specific toxicity of each salt and the interactions among them are the major effects on microbial activity in biological wastewater treatments rather than the osmotic pressure caused by the final salinity.

Experimental-design-guided approach for the removal of atrazine by sono-electrochemical-UV-chlorine techniques.

The aim of the present study was to investigate the electrochemical formation of free chlorine species (HOCl/ClO-) and their subsequent use for the degradation of the pesticide atrazine. Initially, the process of electrochemical-free chlorine production was investigated using a bench-scale electrochemical flow-cell. The most significant variables (electrolyte concentration ([NaCl]) and inter-electrode gap) of the process were obtained using a 23 factorial design and the optimum process conditions (1.73 mol L-1 and 0.56 cm) were determined by a central composite design. Following optimization of free chlorine production, three degradation techniques were investigated, individually and in combination, for atrazine degradation: electrochemical, photochemical and sonochemical. The method using the techniques in combination was denominated sono-photo-assisted electrochemical degradation. Constant current assays were performed and the sono-photo-assisted electrochemical process promoted more efficient removal of atrazine, achieving total organic carbon removal of ∼98% and removal of atrazine to levels below the detection limit (>99%) in under 30 min of treatment. Furthermore, the combination of three techniques displayed lower energy consumption, and phytotoxicity tests (Lactuca sativa) showed that there was no increase in toxicity.

Microwave-enhanced UV/H2O2 degradation of an azo dye (tartrazine): optimization, colour removal, mineralization and ecotoxicity.

This study optimizes two factors, pH and initial [H2O2], in the ultraviolet (UV)/H2O2/microwave (MW) process through experimental design and assesses the effect of MWs on the colour removal of an azo-dye (tartrazine) solution that was favoured by an acidic pH. The estimated optimal conditions were: initial [H2O2] = 2.0 mmol L(-1) and pH = 2.6, at 30 +/- 2 degrees C. We obtained colour removals of approximately 92% in 24 min of irradiation (EDL, 244.2 W), following zero order kinetics: k = (3.9 +/- 0.52) x 10(-2) a.u. min(-1) and R2 = 0.989. Chemical and biological oxygen demand were significantly removed. On the other hand, the carbon content, biodegradability and ecotoxicity (Lactuca sativa) remained approximately the same. The UV/H2O2/MW process was shown to be eight times faster than other tested processes (MW, H2O2, H2O2/MW, and UV/MW).

Lumped kinetics and acute toxicity of intermediates in the ozonation of phenol in saline media.

This work investigates the feasibility of ozonation for destroying phenol and removing organic matter in saline media. The reaction lumped kinetics was followed using the GLKM (General Lumped Kinetic Model). The main intermediate compounds were: catechol, hydroquinone, 4,4'-dihydroxybiphenyl, and 4-bromophenol. It could be noted no significant differences in phenol degradation, mineralization rates, and toxicity removal up to 2 g L(-1) of salt. So, ozonation appears to be a technology that can be used in low salinity media, which is characteristic of waters destined to reuse and recycling programs inside industries.