Towards the description of livestock mobility in Sahelian Africa: Some results from a survey in Mauritania.

Understanding spatio-temporal patterns of host mobility is a key factor to prevent and control animal and human diseases. This is utterly important in low-income countries, where animal disease epidemics have strong socio-economic impacts. In this article we analyzed a livestock mobility database, whose data have been collected by the Centre National d'Elevage et de Recherches Vétérinaires (CNERV) Mauritania, to describe its patterns and temporal evolution. Data were collected through phone and face-to-face interviews in almost all the regions in Mauritania over a period of roughly two weeks during June 2015. The analysis has shown the existence of two mobility patterns throughout the year: the first related to routine movements from January to August; the second strictly connected to the religious festivity of Tabaski that in 2014 occurred at the beginning of October. These mobility patterns are different in terms of animals involved (fewer cattle and dromedaries are traded around Tabaski), the means of transportation (the volume of animals moved by truck raises around Tabaski) and destinations (most of the animals are traded nationally around Tabaski). Due to the differences between these two periods, public health officers, researchers and other stakeholders should take account of the time of the year when implementing vaccination campaigns or creating surveillance networks.

Bluetongue virus surveillance in the Islamic Republic of Mauritania: Is serotype 26 circulating among cattle and dromedaries?

In March 2013, EDTA-blood and serum samples were collected from 119 cattle and 159 dromedaries at the slaughterhouse of Nouakchott, the capital city of the Islamic Republic of Mauritania. Serum samples were screened for the presence of Bluetongue (BT) antibodies by competitive ELISA (cELISA). Positive samples were then tested by serum-neutralization (SN) to determine BTV serotype. RNA from blood samples was first tested by a genus-specific quantitative RT-PCR assay which is able to detect all 27 existing BTV serotypes (RT-qPCR1-27). Positive samples were further screened by a RT-qPCR assay which, instead, is able to detect the classical 24 BTV serotypes only (RT-qPCR1-24). Of the 278 serum samples tested, 177 (mean=63.7%; 95% CI: 57.9%-69.1%) resulted positive by cELISA. Of these, 69 were from cattle (mean=58.0%; 95% CI: 49.0%-66.5%) and 108 from dromedaries (mean=67.9%; 95% CI: 60.3%-74.7%). BTV-26 neutralizing antibodies were by far the most frequently found as they were detected in 146 animals with titres ranging from 1:10 to 1:80. Out of 278 blood samples, 25 (mean=9.0%; 95% CI: 6.2%-12.9%) were found positive for BTV by RT-qPCR1-27, 20 (mean=16.8%; 95% CI: 11.2%-24.6%) were from cattle and 5 (mean=3.1%; 95% CI: 1.4%-7.1%) from dromedaries. When tested by RT-qPCR1-24 the 25 BTV positive samples were negative. Unfortunately, no genetic information by molecular typing or by next generation sequencing has been obtained as for the very low levels of RNA in the blood samples.