RESUMO
The X-linked RP3 gene codes for the ciliary protein RPGR and accounts for over 10% of inherited retinal degenerations. The critical RPGR-ORF15 splice variant contains a highly repetitive purine-rich linker region that renders it unstable and difficult to adapt for gene therapy. To test the hypothesis that the precise length of the linker region is not critical for function, we evaluated whether adeno-associated virus-mediated replacement gene therapy with a human ORF15 variant containing in-frame shortening of the linker region could reconstitute RPGR function in vivo. We delivered human RPGR-ORF15 replacement genes with deletion of most (314 codons, 'short form') or 1/3 (126 codons, 'long form') of the linker region to Rpgr null mice. Human RPGR-ORF15 expression was detected post treatment with both forms of ORF15 transgenes. However, only the long form correctly localized to the connecting cilia and led to significant functional and morphological rescue of rods and cones. Thus the highly repetitive region of RPGR is functionally important but that moderate shortening of its length, which confers the advantage of added stability, preserves its function. These findings provide a theoretical basis for optimizing replacement gene design in clinical trials for X-linked RP3.
Assuntos
Dependovirus/genética , Proteínas do Olho/genética , Terapia Genética , Retinose Pigmentar/terapia , Processamento Alternativo , Animais , Modelos Animais de Doenças , Receptor Quinase 1 Acoplada a Proteína G/genética , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Regiões Promotoras Genéticas , Células Fotorreceptoras Retinianas Cones/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Retinose Pigmentar/genéticaRESUMO
Aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) is required for the biosynthesis of photoreceptor phosphodiesterase (PDE). Gene defects in AIPL1 cause a heterogeneous set of conditions ranging from Leber's congenital amaurosis (LCA), the severest form of early-onset retinal degeneration, to milder forms such as retinitis pigmentosa (RP) and cone-rod dystrophy. In mice, null and hypomorphic alleles cause retinal degeneration similar to human LCA and RP, respectively. Thus these mouse models represent two ends of the disease spectrum associated with AIPL1 gene defects in humans. We evaluated whether adeno-associated virus (AAV)-mediated gene replacement therapy in these models could restore PDE biosynthesis in rods and cones and thereby improve photoreceptor survival. We validated the efficacy of human AIPL1 (isoform 1) replacement gene controlled by a promoter derived from the human rhodopsin kinase (RK) gene, which is active in both rods and cones. We found substantial and long-term rescue of the disease phenotype as a result of transgene expression. This is the first gene therapy study in which both rods and cones were targeted successfully with a single photoreceptor-specific promoter. We propose that the vector and construct design used in this study could serve as a prototype for a human clinical trial.
Assuntos
Proteínas de Transporte/genética , Receptor Quinase 1 Acoplada a Proteína G/genética , Terapia Genética , Atrofia Óptica Hereditária de Leber/terapia , Degeneração Retiniana/terapia , Células Fotorreceptoras Retinianas Bastonetes/metabolismo , Proteínas Adaptadoras de Transdução de Sinal , Animais , Proteínas do Olho , Técnicas de Transferência de Genes , Humanos , Camundongos , Diester Fosfórico Hidrolases/metabolismo , Células Fotorreceptoras Retinianas Cones/metabolismoRESUMO
The crystal structure of three mutants of Escherichia coli alkaline phosphatase with catalytic activity (k(cat)) enhancement as compare to the wild-type enzyme is described in different states. The biological aspects of this study have been reported elsewhere. The structure of the first mutant, D330N, which is threefold more active than the wild-type enzyme, was determined with phosphate in the active site, or with aluminium fluoride, which mimics the transition state. These structures reveal, in particular, that this first mutation does not alter the active site. The second mutant, D153H-D330N, is 17-fold more active than the wild-type enzyme and activated by magnesium, but its activity drops after few days. The structure of this mutant was solved under four different conditions. The phosphate-free enzyme was studied in an inactivated form with zinc at site M3, or after activation by magnesium. The comparison of these two forms free of phosphate illustrates the mechanism of the magnesium activation of the catalytic serine residue. In the presence of magnesium, the structure was determined with phosphate, or aluminium fluoride. The drop in activity of the mutant D153H-D330N could be explained by the instability of the metal ion at M3. The analysis of this mutant helped in the design of the third mutant, D153G-D330N. This mutant is up to 40-fold more active than the wild-type enzyme, with a restored robustness of the enzyme stability. The structure is presented here with covalently bound phosphate in the active site, representing the first phosphoseryl intermediate of a highly active alkaline phosphatase. This study shows how structural analysis may help to progress in the improvement of an enzyme catalytic activity (k(cat)), and explains the structural events associated with this artificial evolution.
Assuntos
Fosfatase Alcalina/química , Fosfatase Alcalina/metabolismo , Evolução Molecular Direcionada , Escherichia coli/enzimologia , Mutação/genética , Fosfatase Alcalina/genética , Sítios de Ligação , Catálise/efeitos dos fármacos , Cristalografia por Raios X , Ativação Enzimática/efeitos dos fármacos , Estabilidade Enzimática , Escherichia coli/genética , Cinética , Magnésio/farmacologia , Modelos Moleculares , Conformação Proteica , Zinco/metabolismoRESUMO
A full-length human androgen receptor (hAR) cDNA was used to produce recombinant baculovirus. Spodoptera frugiperda (Sf9) cells infected with this virus expressed protein with an N-terminal hexahistidine tag (His(6)-hAR) in soluble and insoluble forms. The soluble cytosolic His(6)-hAR demonstrated similar association and dissociation half-times for mibolerone, similar binding affinity for mibolerone, and similar steroid specificity as bona fide AR. Under native conditions, the soluble cytosolic His(6)-hAR was purified to apparent homogeneity in the presence of dihydrotestosterone, using metal ion affinity chromatography. The insoluble pellet fraction was solubilized with strong denaturant 6 M guanidine HCl, and His(6)-hAR was purified from it in the presence of 6 M guanidine HCl. Both the solubilized crude pellet fraction and the solubilized/purified His(6)-hAR could be renatured to bind mibolerone. The baculovirus system will therefore provide an efficient means for producing hAR for ligand-binding assays, as well as purifying hAR for detailed molecular analyses.
Assuntos
Receptores Androgênicos/isolamento & purificação , Animais , Baculoviridae/genética , Células Cultivadas , Cromatografia de Afinidade , Histidina/genética , Humanos , Cinética , Nandrolona/análogos & derivados , Nandrolona/metabolismo , Desnaturação Proteica , Receptores Androgênicos/genética , Receptores Androgênicos/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Spodoptera/genética , Esteroides/metabolismo , Frações Subcelulares/metabolismo , Congêneres da Testosterona/metabolismo , TransfecçãoRESUMO
Hybridoma lines producing anti-idiotypic monoclonal antibodies (AImAbs) were prepared by fusing splenocytes of mice immunized with alpha-latrotoxin (LT) and P3-X63Ag8.653 myeloma line cells. AImAbs (1) bind to the rat brain synaptosomes, (2) do not affect the LT binding to the high-affinity receptor, and (3) do not react with LT in solution. The effect of AImAbs on the LT-induced 45Ca2+ influx into rat brain synaptosomes was studied. Some antibodies (6.6D11 and 11.7B7) were found to strongly inhibit this process. The result obtained indicate that the presynaptic membrane contains unidentified components interacting with LT. The distortion of the interaction of LT with these unknown components affects the LT-induced calcium influx into synaptosomes.
Assuntos
Anticorpos Anti-Idiotípicos/metabolismo , Anticorpos Monoclonais/imunologia , Encéfalo/imunologia , Cálcio/metabolismo , Venenos de Aranha/imunologia , Sinaptossomos/imunologia , Animais , Sítios de Ligação de Anticorpos , Encéfalo/ultraestrutura , Feminino , Hibridomas , Transporte de Íons , Camundongos , Camundongos Endogâmicos BALB C , Proteínas do Tecido Nervoso/metabolismo , Ratos , Receptores de Peptídeos/metabolismo , Venenos de Aranha/metabolismo , Venenos de Aranha/farmacologia , Sinaptossomos/efeitos dos fármacos , Sinaptossomos/metabolismoRESUMO
The N-terminal amino acid sequence of alpha-latroinsectotoxin from the venom of Latrodectus mactans tredecimguttatus was determined. Then the toxin was digested by trypsin and total or partial amino acid sequences of twenty-six tryptic peptides were established. This resulted in the structural information needed for the construction of probes followed by the cloning of the alpha-latroinsectotoxin structural gene.
Assuntos
Viúva Negra/metabolismo , Venenos de Aranha/genética , Sequência de Aminoácidos , Animais , Cromatografia Líquida de Alta Pressão , Clonagem Molecular , Genes , Dados de Sequência Molecular , Mapeamento de Peptídeos , TripsinaRESUMO
A panel of monoclonal antibodies (mAb) against alpha-latrotoxin (LT) has been produced and their main characteristics have been determined. The influence of mAb on the functional effects of LT in synaptosomes from rat brain and on the channel formation in bilayer lipid membrane has been investigated. These mAbs do not inhibit binding of LT to rat synaptosomes but modify LT-receptor interaction in terms of LT's channel-forming and secretogenic effects. Antibodies A6 and A24 block these effects, whereas A4 partially preserves the secretogenic action of LT and completely blocks its channel-forming action. Only antibodies A15 affect the LT ability to form cationic channels in BLM, inducing considerable decrease in the frequency of the channel formation. These data and their analysis allow to identify several functional (and, probably, structural) domains of LT responsible for: 1) toxin-receptor interaction; 2) channel-forming and related calcium-dependent secretogenic effects; 3) calcium-independent secretogenic effects; 4) formation of cationic channels in the artificial lipid bilayer.
Assuntos
Venenos de Aranha/metabolismo , Animais , Anticorpos Monoclonais , Encéfalo/metabolismo , Cálcio/metabolismo , Cátions Bivalentes , Imunoquímica , Canais Iônicos/metabolismo , Bicamadas Lipídicas , Ratos , Sinaptossomos/metabolismo , Ácido gama-Aminobutírico/metabolismoRESUMO
A homogenous protein of 120,000 mol. wt isolated from black widow spider (Lactrodectus mactans tredecimguttatus) venom and referred to as alpha-latroinsectotoxin was highly potent (4 nM) in the induction of an increase of the frequency of miniature excitatory postsynaptic potentials in blowfly (Calliphora vicina) larvae neuromuscular preparations. In the frog nerve ending, however, even 50 nM alpha-latroinsectotoxin failed to affect transmitter release. Pretreatment of insect preparations with alpha-latrotoxin or frog preparations with alpha-latroinsectotoxin did not prevent the specific effect of consequent applications of alpha-latroinsectotoxin (insect) and alpha-latrotoxin (frog), respectively. The binding of labelled [125I]alpha-latroinsectotoxin to insect and [125I]alpha-latrotoxin to bovine membrane preparations was saturable and highly specific. The presynaptic effect, but not the binding of alpha-latroinsectotoxin, was dependent on the presence of divalent cations in the external medium. Mg2+ could readily substitute for Ca2+ and increase of transmitter release induced by alpha-latroinsectotoxin also occurred in Ca(2+)-free solutions. Pretreatment of preparations with 300 micrograms/ml concanavalin A completely abolished both the presynaptic effect of alpha-latroinsectotoxin and its binding to insect membrane preparations. Thus, the phenomenology of alpha-latroinsectotoxin action on insects resembles in general that described for the action of alpha-latrotoxin on vertebrates. The selectivity of alpha-latrotoxin and alpha-latroinsectotoxin seems to be due to differences in the structure of neurotoxin receptors in nerve endings of vertebrates and insects, although the mode of presynaptic action has a great deal in common.
Assuntos
Viúva Negra/metabolismo , Venenos de Aranha/isolamento & purificação , Animais , Anuros , Encéfalo/metabolismo , Cálcio/farmacologia , Bovinos , Concanavalina A/farmacologia , Dípteros , Gryllidae , Immunoblotting , Técnicas In Vitro , Placa Motora/metabolismo , Junção Neuromuscular/efeitos dos fármacos , Venenos de Aranha/farmacocinética , Venenos de Aranha/farmacologia , Sinapses/efeitos dos fármacosRESUMO
The crude venom of spider Latrodectus mactans tredecimguttatus was fractionated by the combination of anion exchange and hydrophobic chromatography. The biological activity of fraction was tested by means of: 1) estimation of toxicity for housefly larva; 2) intracellular recording of miniature excitatory potentials (MEPSPs) in blowfly larvae muscle fibres. As a result of sequential procedures of chromatography separation a homogeneous protein of 120 kilodalton molecular weight was obtained. This protein referred to alpha-latroinsectotoxin produced: 1) a great increase of the frequency of MEPSPs in the dose of 4.2.10(-10) M and its paralytic dose for fly larva was approximately 20 ng/species; 2) no influence of the MEPSPs after application in the dose of 1.2.10(-7) M to the neuromuscular junction of the frog.
Assuntos
Venenos de Aranha/isolamento & purificação , Aminoácidos/química , Animais , Anuros , Viúva Negra , Cromatografia por Troca Iônica , Dípteros , Eletroforese em Gel de Poliacrilamida , Potenciais Evocados/efeitos dos fármacos , Técnicas In Vitro , Venenos de Aranha/química , Venenos de Aranha/toxicidadeRESUMO
A method of alpha-latrotoxin (LT) isolation from the venom of Latrodectus mactans tredecimguttatus by means of immunoaffinity chromatography on sepharose conjugated with monoclonal antibodies against LT has been developed. This one-step, high-yield, relatively simple and rapid procedure yields active LT for structural and functional studies of its receptor.