RESUMO
Neurodegenerative diseases are characterized by a decline in neuronal function and structure, leading to neuronal death. Understanding the molecular mechanisms of neuronal death is crucial for developing therapeutics. MiRs are small noncoding RNAs that regulate gene expression by degrading target mRNAs or inhibiting translation. MiR dysregulation has been linked to many neurodegenerative diseases, but the underlying mechanisms are not well understood. As mitochondrial dysfunction is one of the common molecular mechanisms leading to neuronal death in many neurodegenerative diseases, here we studied miRs that modulate neuronal death caused by 1-methyl-4-phenylpyridinium (MPP+), an inhibitor of complex I in mitochondria. We identified miR-593-5p, levels of which were increased in SH-SY5Y human neuronal cells, after exposure to MPP+. We found that intracellular Ca2+, but not of reactive oxygen species, mediated this miR-593-5p increase. Furthermore, we found the increase in miR-593-5p was due to enhanced stability, not increased transcription or miR processing. Importantly, we show the increase in miR-593-5p contributed to MPP+-induced cell death. Our data revealed that miR-593-5p inhibits a signaling pathway involving PTEN-induced putative kinase 1 (PINK1) and Parkin, two proteins responsible for the removal of damaged mitochondria from cells, by targeting the coding sequence of PINK1 mRNA. Our findings suggest that miR-593-5p contributes to neuronal death resulting from MPP+ toxicity, in part, by impeding the PINK1/Parkin-mediated pathway that facilitates the clearance of damaged mitochondria. Taken together, our observations highlight the potential significance of inhibiting miR-593-5p as a therapeutic approach for neurodegenerative diseases.
Assuntos
MicroRNAs , Neuroblastoma , Proteínas Quinases , Humanos , 1-Metil-4-fenilpiridínio/toxicidade , Apoptose , Morte Celular , Linhagem Celular Tumoral , MicroRNAs/genética , MicroRNAs/metabolismo , Neuroblastoma/metabolismo , Proteínas Quinases/genética , Proteínas Quinases/metabolismo , Ubiquitina-Proteína Ligases/genéticaRESUMO
Alpha-Synuclein (α-Syn) is an important protein in the pathogenesis of Parkinson disease (PD) as it accumulates as fibrillar inclusions in affected brain regions including dopaminergic neurons in the substantia nigra. Elevated levels of α-Syn seem to be crucial in mediating its toxicity. Thus, detailed information regarding the regulatory mechanism of α-Syn expression in several layers such as transcription, post-transcription and post-translation is needed in order to devise therapeutic interventions for PD. Previously, we reported that expression of α-Syn is repressed by microRNA-7 (miR-7) through its effect on the 3'-untranslated region (UTR) of α-Syn mRNA. Here, we show that miR-7 also accelerates the clearance of α-Syn and its aggregates by promoting autophagy in differentiated ReNcell VM cells. Further, miR-7 facilitates the degradation of pre-formed fibrils of α-Syn transported from outside the cells. This additional mechanism for reducing α-Syn levels show miR-7 to be an important molecular target for PD and other alpha-synucleinopathies.
Assuntos
Autofagia , MicroRNAs/metabolismo , Agregação Patológica de Proteínas , Proteólise , alfa-Sinucleína/metabolismo , Células Cultivadas , Neurônios Dopaminérgicos/metabolismo , Células HEK293 , Humanos , Células-Tronco Neurais/metabolismoRESUMO
In this paper a novel filtering scheme combined with a lighting method is proposed for defect detection in steel surfaces. A steel surface has non-uniform brightness and various shaped defects, which cause difficulties in defect detection. To solve this problem we propose a sub-optimal filtering that is combined with a switching-lighting method. First, dual-light switching lighting (DLSL) is explained, which decreases the effect of non-uniformity of surface brightness and improves the detection accuracy. By using the DLSL method, defects are represented as alternated black and white patterns regardless of the size, shape, or orientation of defects. Therefore, defects can be detected by finding alternated black and white patterns. Second, we propose a scheme for detecting defects in steel-surface images acquired using the DLSL method. The presence of scales strongly affects the optical properties of the surface. Moreover, the textures of steel-plate images vary greatly because of the temperature and grade of steel. Therefore, conventional filter-design methods are not effective for different image textures. A sub-optimal scheme based on an optimized general-finite impulse-response filter is also proposed. Finally, experimental results conducted on steel-surface images from an actual steel-production line show the effectiveness of the proposed algorithm.
RESUMO
Mitochondrial dysfunction is one of the major contributors to neurodegenerative disorders including Parkinson disease. The mitochondrial permeability transition pore is a protein complex located on the mitochondrial membrane. Under cellular stress, the pore opens, increasing the release of pro-apoptotic proteins, and ultimately resulting in cell death. MicroRNA-7 (miR-7) is a small non-coding RNA that has been found to exhibit a protective role in the cellular models of Parkinson disease. In the present study, miR-7 was predicted to regulate the function of mitochondria, according to gene ontology analysis of proteins that are down-regulated by miR-7. Indeed, miR-7 overexpression inhibited mitochondrial fragmentation, mitochondrial depolarization, cytochrome c release, reactive oxygen species generation, and release of mitochondrial calcium in response to 1-methyl-4-phenylpyridinium (MPP(+)) in human neuroblastoma SH-SY5Y cells. In addition, several of these findings were confirmed in mouse primary neurons. Among the mitochondrial proteins identified by gene ontology analysis, the expression of voltage-dependent anion channel 1 (VDAC1), a constituent of the mitochondrial permeability transition pore, was down-regulated by miR-7 through targeting 3'-untranslated region of VDAC1 mRNA. Similar to miR-7 overexpression, knockdown of VDAC1 also led to a decrease in intracellular reactive oxygen species generation and subsequent cellular protection against MPP(+). Notably, overexpression of VDAC1 without the 3'-UTR significantly abolished the protective effects of miR-7 against MPP(+)-induced cytotoxicity and mitochondrial dysfunction, suggesting that the protective effect of miR-7 is partly exerted through promoting mitochondrial function by targeting VDAC1 expression. These findings point to a novel mechanism by which miR-7 accomplishes neuroprotection by improving mitochondrial health.
Assuntos
MicroRNAs/genética , Interferência de RNA , Canal de Ânion 1 Dependente de Voltagem/metabolismo , Regiões 3' não Traduzidas , Animais , Sequência de Bases , Sítios de Ligação , Linhagem Celular Tumoral , Expressão Gênica , Ontologia Genética , Humanos , Potencial da Membrana Mitocondrial , Camundongos Endogâmicos C57BL , Proteínas de Transporte da Membrana Mitocondrial/metabolismo , Membranas Mitocondriais/metabolismo , Poro de Transição de Permeabilidade Mitocondrial , Tamanho Mitocondrial , Espécies Reativas de Oxigênio/metabolismo , Canal de Ânion 1 Dependente de Voltagem/genéticaRESUMO
Nuclear factor E2-related factor 2 (Nrf2) is a key transcription factor that regulates the expression of a number of antioxidant and detoxifying genes that provide cellular protection against various stressors including reactive oxygen species (ROS). Nrf2 activity is tightly regulated by a cytoplasmic inhibitory protein called Kelch-like ECH-associated protein 1 (Keap1). The mechanism that controls Keap1 expression, however, remains poorly understood. In the present study, we demonstrate that microRNA-7 (miR-7), which is highly expressed in the brain, represses Keap1 expression by targeting the 3'-untranslated region (UTR) of its mRNA in human neuroblastoma cells, SH-SY5Y. Subsequently, this event results in an increased Nrf2 activity, as evidenced by an increase in the expression of its transcriptional targets, heme oxygenase 1 (HO-1) and glutamate-cysteine ligase modifier subunit (GCLM), and an enhanced nuclear localization of Nrf2. In addition, miR-7 decreases the intracellular hydroperoxides level and increases the level of reduced form of glutathione, indicative of oxidative stress relief. We also demonstrate that targeted repression of Keap1 and activation of Nrf2 pathway, in part, underlies the protective effects of miR-7 against 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity in SH-SY5Y and differentiated human neural progenitor cells, ReNcell VM. These findings point to a new mechanism by which miR-7 exerts cytoprotective effects by regulating the Nrf2 pathway.
Assuntos
Regulação da Expressão Gênica/fisiologia , Peptídeos e Proteínas de Sinalização Intracelular/biossíntese , MicroRNAs/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo/fisiologia , Western Blotting , Linhagem Celular , Cromatografia Líquida , Humanos , Proteína 1 Associada a ECH Semelhante a Kelch , Reação em Cadeia da Polimerase , Transdução de Sinais/fisiologia , Espectrometria de Massas em Tandem , TransfecçãoRESUMO
Parkinson disease is associated with decreased activity of the mitochondrial electron transport chain. This defect can be recapitulated in vitro by challenging dopaminergic cells with 1-methyl-4-phenylpyridinium (MPP(+)), a neurotoxin that inhibits complex I of electron transport chain. Consequently, oxidative phosphorylation is blocked, and cells become dependent on glycolysis for ATP production. Therefore, increasing the rate of glycolysis might help cells to produce more ATP to meet their energy demands. In the present study, we show that microRNA-7, a non-coding RNA that protects dopaminergic neuronal cells against MPP(+)-induced cell death, promotes glycolysis in dopaminergic SH-SY5Y and differentiated human neural progenitor ReNcell VM cells, as evidenced by increased ATP production, glucose consumption, and lactic acid production. Through a series of experiments, we demonstrate that targeted repression of RelA by microRNA-7, as well as subsequent increase in the neuronal glucose transporter 3 (Glut3), underlies this glycolysis-promoting effect. Consistently, silencing Glut3 expression diminishes the protective effect of microRNA-7 against MPP(+). Further, microRNA-7 fails to prevent MPP(+)-induced cell death when SH-SY5Y cells are cultured in a low glucose medium, as well as when differentiated ReNcell VM cells or primary mouse neurons are treated with the hexokinase inhibitor, 2-deoxy-d-glucose, indicating that a functional glycolytic pathway is required for this protective effect. In conclusion, microRNA-7, by down-regulating RelA, augments Glut3 expression, promotes glycolysis, and subsequently prevents MPP(+)-induced cell death. This protective effect of microRNA-7 could be exploited to correct the defects in oxidative phosphorylation in Parkinson disease.
Assuntos
1-Metil-4-fenilpiridínio/química , Morte Celular , MicroRNAs/metabolismo , Fator de Transcrição RelA/metabolismo , Trifosfato de Adenosina/química , Animais , Diferenciação Celular , Linhagem Celular Tumoral , Sobrevivência Celular , Desoxiglucose/química , Glucose/química , Transportador de Glucose Tipo 3/metabolismo , Glicólise , Células HEK293 , Humanos , Ácido Láctico/química , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/metabolismo , Neurônios/metabolismo , Fosforilação Oxidativa , Oxigênio/química , Doença de Parkinson/metabolismo , FosforilaçãoRESUMO
Mounting evidence suggests that microRNA (miR) dysregulation contributes to neurodegenerative disorders including Parkinson's disease (PD). MiR-34b and miR-34c have been previously shown to be down-regulated in the brains of patients with PD. Here, we demonstrate that miR-34b and miR-34c repress the expression of α-synuclein (α-syn), a key protein in PD pathogenesis. Inhibition of miR-34b and miR-34c expression in human dopaminergic SH-SY5Y cells increased α-syn levels and stimulated aggregate formation. Additionally, a single nucleotide polymorphism (SNP) in the 3'-UTR of α-syn was found to lower the miR-34b-mediated repression of the protein. Our results suggest that down-regulation of miR-34b and miR-34c in the brain, as well as an SNP in the 3'-UTR of α-syn can increase α-syn expression, possibly contributing to PD pathogenesis.
Assuntos
MicroRNAs/genética , Doença de Parkinson/genética , alfa-Sinucleína/biossíntese , Regiões 3' não Traduzidas , Encéfalo/metabolismo , Encéfalo/patologia , Linhagem Celular , Neurônios Dopaminérgicos/metabolismo , Neurônios Dopaminérgicos/patologia , Regulação da Expressão Gênica , Humanos , MicroRNAs/antagonistas & inibidores , Doença de Parkinson/patologia , Polimorfismo de Nucleotídeo Único , alfa-Sinucleína/genéticaRESUMO
Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons in the substantia nigra. Mitochondrial complex I impairment in PD is modeled in vitro by the susceptibility of dopaminergic neurons to the complex I inhibitor 1-methyl-4-phenylpyridinium (MPP+). In the present study, we demonstrate that microRNA-7 (miR-7), which is expressed in tyrosine hydroxylase-positive nigral neurons in mice and humans, protects cells from MPP+-induced toxicity in dopaminergic SH-SY5Y cells, differentiated human neural progenitor ReNcell VM cells, and primary mouse neurons. RelA, a component of nuclear factor-κB (NF-κB), was identified to be downregulated by miR-7 using quantitative proteomic analysis. Through a series of validation experiments, it was confirmed that RelA mRNA is a target of miR-7 and is required for cell death following MPP+ exposure. Further, RelA mediates MPP+-induced suppression of NF-κB activity, which is essential for MPP+-induced cell death. Accordingly, the protective effect of miR-7 is exerted through relieving NF-κB suppression by reducing RelA expression. These findings provide a novel mechanism by which NF-κB suppression, rather than activation, underlies the cell death mechanism following MPP+ toxicity, have implications for the pathogenesis of PD, and suggest miR-7 as a therapeutic target for this disease.
Assuntos
1-Metil-4-fenilpiridínio/antagonistas & inibidores , MicroRNAs/fisiologia , Fármacos Neuroprotetores/metabolismo , Doença de Parkinson Secundária/prevenção & controle , Fator de Transcrição RelA/biossíntese , 1-Metil-4-fenilpiridínio/toxicidade , Animais , Morte Celular/efeitos dos fármacos , Morte Celular/fisiologia , Células Cultivadas , Neurônios Dopaminérgicos/efeitos dos fármacos , Neurônios Dopaminérgicos/fisiologia , Regulação para Baixo , Humanos , Camundongos , MicroRNAs/biossíntese , MicroRNAs/genética , NF-kappa B/biossíntese , Neurônios/efeitos dos fármacos , Doença de Parkinson Secundária/induzido quimicamente , Substância Negra/metabolismo , Fator de Transcrição RelA/genética , Transfecção , alfa-Sinucleína/genéticaRESUMO
Presently, product inspection based on vision systems is an important part of the steel-manufacturing industry. In this work, we focus on the detection of seam cracks in the edge region of steel plates. Seam cracks are generated in the vertical direction, and their width range is 0.2-0.6 mm. Moreover, the gray values of seam cracks are only 20-30 gray levels lower than those of the neighboring surface. Owing to these characteristics, we propose a new algorithm for detecting seam cracks using a Gabor filter combination method. To enhance the performance, we extracted features of seam cracks and employed a support vector machine classifier. The experimental results show that the proposed algorithm is suitable for detecting seam cracks.
RESUMO
Presently, automatic inspection algorithms are widely used to ensure high-quality products and achieve high productivity in the steelmaking industry. In this paper, we propose a vision-based method for detecting corner cracks on the surface of steel billets. Because of the presence of scales composed of oxidized substances, the billet surfaces are not uniform and vary considerably with the lighting conditions. To minimize the influence of scales and improve the accuracy of detection, a detection method based on a visual inspection algorithm is proposed. Wavelet reconstruction is used to reduce the effect of scales. Texture and morphological features are used to identify the corner cracks among the defective candidates. Finally, the experimental results show that the proposed algorithm is effective in detecting corner cracks on the surfaces of the steel billets.
RESUMO
We propose a new defect detection algorithm for scale-covered steel wire rods. The algorithm incorporates an adaptive wavelet filter that is designed on the basis of lattice parameterization of orthogonal wavelet bases. This approach offers the opportunity to design orthogonal wavelet filters via optimization methods. To improve the performance and the flexibility of wavelet design, we propose the use of the undecimated discrete wavelet transform, and separate design of column and row wavelet filters but with a common cost function. The coefficients of the wavelet filters are optimized by the so-called univariate dynamic encoding algorithm for searches (uDEAS), which searches the minimum value of a cost function designed to maximize the energy difference between defects and background noise. Moreover, for improved detection accuracy, we propose an enhanced double-threshold method. Experimental results for steel wire rod surface images obtained from actual steel production lines show that the proposed algorithm is effective.
RESUMO
Presently, product inspection for quality control is becoming an important part in the steel manufacturing industry. In this paper, we propose a vision-based method for detection of pinholes in the surface of scarfed slabs. The pinhole is a very tiny defect that is 1-5 mm in diameter. Because the brightness in the surface of a scarfed slab is not uniform and the size of a pinhole is small, it is difficult to detect pinholes. To overcome the above-mentioned difficulties, we propose a new defect detection algorithm using a Gabor filter and morphological features. The Gabor filter was used to extract defective candidates. The morphological features are used to identify the pinholes among the defective candidates. Finally, the experimental results show that the proposed algorithm is effective to detect pinholes in the surface of the scarfed slab.