1.
J Anat
; 230(4): 601-606, 2017 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28054714
RESUMO
Confocal laser-scanning microscopy is a useful tool for visualizing neurons and glia in transparent preparations of brain tissue from laboratory animals. Currently, imaging capillaries and venules in transparent brain tissues requires the use of fluorescent proteins. Here, we show that vessels can be imaged by confocal laser-scanning microscopy in transparent cortical, hippocampal and cerebellar preparations after clarification of China ink-injected specimens by the Spalteholz method. This method may be suitable for global, three-dimensional, quantitative analyses of vessels, including stereological estimations of total volume and length and of surface area of vessels, which constitute indirect approaches to investigate angiogenesis.
Assuntos
Química Encefálica , Encéfalo/irrigação sanguínea , Circulação Cerebrovascular , Tinta , Coloração e Rotulagem/métodos , Animais , Encéfalo/fisiologia , Química Encefálica/fisiologia , Circulação Cerebrovascular/fisiologia , Masculino , Microscopia Confocal/métodos , Ratos , Ratos Wistar
2.
Ann N Y Acad Sci
; 919: 304-6, 2000.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11083121