RESUMO
By screening of a Leishmania infantum expression library with the serum from a dog affected with visceral leishmaniasis, a cDNA clone with sequence homology to the Hsp83 gene family was isolated. From analysis of the genomic distribution of the cDNA sequence, it was estimated that the L. infantum genome contains 7 Hsp83 genes tandemly organized. The full-length coding region of the Hsp83 gene located at the 5'-end of the cluster was determined. The deduced amino acid sequence of the L. infantum Hsp83 shows a high level of sequence identity with members of the Hsp83's protein family from other eukaryotic organisms. The complete protein (LiHsp83) and 4 subfragments (LiA1, LiB1, LiC1 and LiD1) were expressed in Escherichia coli as recombinant proteins and used as target antigens in FAST-ELISA assays against a collection of sera from dogs with visceral leishmaniasis. Ninety percent of the sera recognized the recombinant LiHsp83, indicating that L. infantum Hsp83 is a potent immunogen during canine leishmaniasis. Serological analysis of the recombinant subfragments identified the LiB1 subfragment, from amino acid 156 to 283, as the immunodominant region of the protein. This region, which is the less evolutionary conserved region of the protein, was recognized by 88% of the visceral leishmaniasis sera. The results suggest that L. infantum Hsp83 and particular protein subfragments may be useful in serodiagnostic assays for canine leishmaniasis.
Assuntos
Doenças do Cão/parasitologia , Proteínas de Choque Térmico/imunologia , Leishmania infantum/imunologia , Leishmaniose Visceral/veterinária , Proteínas de Protozoários/imunologia , Animais , Antígenos de Protozoários/genética , Antígenos de Protozoários/imunologia , Western Blotting , Clonagem Molecular , DNA de Protozoário/análise , Doenças do Cão/imunologia , Cães , Ensaio de Imunoadsorção Enzimática , Genes de Protozoários , Proteínas de Choque Térmico/genética , Leishmania infantum/genética , Leishmania infantum/isolamento & purificação , Leishmaniose Visceral/imunologia , Leishmaniose Visceral/parasitologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Proteínas de Protozoários/genética , Homologia de Sequência de AminoácidosRESUMO
The familial occurrence of pars planitis is rare. We have found ten cases reported previously. We describe a new case of pars planitis in a family. The affected members included a mother and two of her four children. The family was tested for HLA antigens in order to establish a comparison with others HLA types by different authors. We have not identified any cause for the familial occurrence of this disease. We discuss the role of genetic and ambiental factors.
Assuntos
Pars Planite/genética , Criança , Feminino , Antígenos HLA/análise , Humanos , Masculino , Pars Planite/etiologia , LinhagemRESUMO
Although no overt injury occurred, a corneal abscess without hypopyon developed in a normal, 25-year-old female, living in a suburban area of Madrid, following a period of intensive garden work. Direct examination of the corneal scrapings on one occasion showed septate, branched hyphal elements. When scrapings were cultured on Sabouraud glucose and brain heart infusion agars a pure culture of Scedosporium apiospermum was repeatedly isolated. The patient responded successfully to treatment with miconazole ointment, although a residual immune ring of the cornea remained and final visual acuity was 6/10.
