RESUMO
Arabidopsis thaliana (Arabidopsis) is unique among plant model organisms in having a small genome (130-140 Mb), excellent physical and genetic maps, and little repetitive DNA. Here we report the sequence of chromosome 2 from the Columbia ecotype in two gap-free assemblies (contigs) of 3.6 and 16 megabases (Mb). The latter represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date. Chromosome 2 represents 15% of the genome and encodes 4,037 genes, 49% of which have no predicted function. Roughly 250 tandem gene duplications were found in addition to large-scale duplications of about 0.5 and 4.5 Mb between chromosomes 2 and 1 and between chromosomes 2 and 4, respectively. Sequencing of nearly 2 Mb within the genetically defined centromere revealed a low density of recognizable genes, and a high density and diverse range of vestigial and presumably inactive mobile elements. More unexpected is what appears to be a recent insertion of a continuous stretch of 75% of the mitochondrial genome into chromosome 2.
Assuntos
Arabidopsis/genética , Mapeamento Cromossômico , DNA de Plantas , Genes de Plantas , Núcleo Celular/genética , Centrômero , Evolução Molecular , Duplicação Gênica , Genes de Plantas/fisiologia , Mitocôndrias/genética , Dados de Sequência Molecular , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Análise de Sequência de DNAAssuntos
Infarto do Miocárdio/tratamento farmacológico , Terapia Trombolítica/enfermagem , Humanos , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/enfermagem , Avaliação em Enfermagem , Planejamento de Assistência ao Paciente , Terapia Trombolítica/efeitos adversos , Terapia Trombolítica/métodos , Fatores de TempoRESUMO
Recent studies of appropriateness of air medical transport have not specifically examined the need of cardiac patients for specialized services not available in the original community setting. This study examined the subsequent use of specialized services (i.e., coronary bypass surgery, angioplasty) by 796 consecutively aeromedically transported cardiac patients as an indicator of appropriate use of air transport. At least one specialized service was received by 552 (69.3%) patients. Preflight or transport interventions outside the clinical scope of the available ground transport system--a narrow, but objective assessment of appropriateness--were received by 507 patients and were associated with subsequent receipt of posttransport specialized services (p less than 0.0001). We conclude that the use of specialized services by cardiac patients was high and associated with an objective measure of appropriateness of transport. Studies of aeromedical transport should consider the need for specialized services in addition to intensity of care in the determination of appropriate use.
