RESUMO
Growth of Saccharomyces cerevisiae in minimal salts-glucose-SO4(2-) medium with varying concentrations of pantothenate (0-1000 microgram/L) produced changes in the cellular lipid content and in the ratio of saturated to unsaturated fatty acids. Substantial differences in SO4(2-) diffusion were also observed with changes in pantothenate concentration. During sulfate reduction, the delta 34S value of the evolved sulfide varied with the pantothenate concentration ranging from -31% in the absence of pantothenate to 0% at 400-1000 microgram/L pantothenate. The isotope selectivity is related to the effect of pantothenate concentration on cellular metabolism.
Assuntos
Ácido Pantotênico/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Sulfatos/metabolismo , Ácidos Graxos/biossíntese , Ácidos Graxos Insaturados/biossíntese , Lipídeos/biossíntese , Metais/farmacologia , Saccharomyces cerevisiae/metabolismo , Sulfetos/metabolismo , Isótopos de EnxofreAssuntos
Saccharomyces cerevisiae/metabolismo , Sulfatos/metabolismo , Transporte Biológico Ativo , Radioisótopos de Carbono , Cromo/farmacologia , Glucose/metabolismo , Glucose/farmacologia , Cinética , Metionina/metabolismo , Molibdênio/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Selênio/farmacologia , Espectrofotometria , Sulfitos/farmacologia , Radioisótopos de Enxofre , Telúrio/farmacologia , Temperatura , Tiossulfatos/farmacologiaRESUMO
The ribosome content was markedly reduced when illumination (at 28 C) was repeatedly interrupted; an opposite trend was observed with continuous light as the temperature was decreased.
