RESUMO
The effects of 24R, 25-dihydroxyvitamin D3 (24, 25 (OH)2 D3) on alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT) and acid phosphatase (ACP) activities were investigated on renal cortex of hypophysectomized (Hx) rats. ALP activity was increased by +27, +56 and +60% as compared to controls respectively 3, 6 and 12 h after intraperitoneal administration of the secosteroid (10 pmoles/100 g body weight). Stimulations of GGT activity began only after 6 h (+30%) and 12 h (+ 46%). ACP activity was not modified. In vivo, the two enzymatic inductions in kidneys of Hx rats were higher and longer than those obtained in vitro.
Assuntos
24,25-Di-Hidroxivitamina D 3/farmacologia , Fosfatase Alcalina/efeitos dos fármacos , Rim/efeitos dos fármacos , Hipófise/fisiologia , gama-Glutamiltransferase/efeitos dos fármacos , Animais , Hipofisectomia , Rim/enzimologia , Ratos , Ratos Sprague-DawleyRESUMO
The effects of 24R,25-dihydroxyvitamin D3 (24,25-(OH)2D3 on alkaline phosphatase (PAL), gamma-glutamyltransferase (GGT) and acid phosphatase (PAC) activities were investigated on renal cortex slices of hypophysectomized rats. Indeed after hypophysectomy renal 24,25-(OH)2D3 production was increased and renal PAL and GGT activities were decreased. After 5h incubation with physiological concentrations (0.1-10 nM) of 24,25-(OH)2D3 significant increases of PAL and GGT activities were produced. The maximum stimulation obtained with 1 nM was +23% for PAL and +26% for GGT as compared to controls. PAC was not modified. The time course of these effects was studied from 45 min to 8 h. In the presence of 24,25-(OH)2D3 (1 nM), delayed (3h) stimulation of PAL and GGT appeared. It reached the maximal value after 6h, +37% for PAL and +30% for GGT and persisted again at 8h. Cycloheximide added to incubation medium with steroid inhibited the stimulating effect on PAL only. Actinomycin D suppressed the induction of both enzymes, indicating that the observed actions of 24,25-(OH)2D3 depend on protein synthesis whose responsible mechanisms were different. These protein synthesis inhibitors did not modified enzymatic activities. Physiological significance of these renal effects is to be clarified.
Assuntos
24,25-Di-Hidroxivitamina D 3/farmacologia , Fosfatase Alcalina/metabolismo , Hipofisectomia , Córtex Renal/enzimologia , gama-Glutamiltransferase/metabolismo , Fosfatase Ácida/metabolismo , Animais , Cicloeximida/farmacologia , Dactinomicina/farmacologia , Feminino , Córtex Renal/efeitos dos fármacos , Cinética , Ratos , Ratos EndogâmicosRESUMO
Effects in vitro of 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) on alkaline phosphatase (PAL), gamma-glutamyltransferase (gamma-GT) and acid phosphatase (PAC) activities were investigated on renal cortex from hypophysectomized rats. In these animals the biosynthesis of 1,25-(OH)2D3 and the specific activities of kidney PAL and gamma-GT were decreased. The course of these effects was determined from 45 min to 8 h. In the presence of 1,25-(OH)2D3 (2 x 10(-6) M) a delayed (5h) but simultaneous stimulation of the three enzymes was observed. It reached a maximum at 6h and disappeared at 8h. The dose-response relation was studied at 6h. In the presence of 1,25-(OH)2D3 (5 x 10(-7) M), the three enzymes were activated. The effect was maximal at 10(-6) M; it was +22% for PAL, +17% and +15% respectively for gamma-GT and PAC compared with controls. Cycloheximide suppressed the induction of PAL but not of gamma-GT activity. The effects of the secosteroid on renal enzymes seems to be a pharmacological more than a physiological one.