RESUMO
From Astragalus peregrinus, four cycloartane-type saponins have been isolated and their structures elucidated by spectral means as 20(R),24(S)-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,25-tetrol 3-O-beta-D-glucopyranoside (1), 20(R),24(S)-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,25-tetrol 3-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranoside (2), 20(R),24(S)-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,25-tetrol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside (3) and 20(R),25-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,24(S)-tetrol (24-O-acetyl)- 3-O-alpha-L-rhamnopyranosyl-(1-->2)-(6'-O-acetyl)-beta-D-glucopyranoside (4). Compounds 2 and 3 showed to stimulate the proliferation of mouse splenocytes and were not significantly cytotoxic.
Assuntos
Adjuvantes Imunológicos/farmacologia , Fabaceae , Linfócitos/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Saponinas/farmacologia , Adjuvantes Imunológicos/uso terapêutico , Animais , Feminino , Humanos , Camundongos , Camundongos Endogâmicos , Extratos Vegetais/uso terapêutico , Estruturas Vegetais , Saponinas/uso terapêutico , Baço/citologia , Baço/efeitos dos fármacos , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
Two new antimicrobial isoflavans, 1-[(3R)-7,8-dimethoxybenzopyranyl]-4- hydroxybenzoquinone (astragaluquinone) and (3S)-7,1'-dihydroxy-8,3'-dimethoxyisoflavan (8-methoxyvestitol), and the known 7-hydroxy-2',3',4'-trimethoxyisoflavan were isolated from roots of Astragalus alexandrinus and A. trigonus. The structures of the new isoflavans were established by spectroscopic methods.
Assuntos
Anti-Infecciosos/farmacologia , Antifúngicos/farmacologia , Benzopiranos/farmacologia , Isoflavonas , Plantas Medicinais/química , Quinonas/farmacologia , Antibacterianos , Anti-Infecciosos/química , Antifúngicos/química , Benzopiranos/química , Benzoquinonas , Candida albicans/efeitos dos fármacos , Sequência de Carboidratos , Klebsiella pneumoniae/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Medicina Tradicional Chinesa , Testes de Sensibilidade Microbiana , Micrococcus luteus/efeitos dos fármacos , Dados de Sequência Molecular , Raízes de Plantas/química , Quinonas/química , Saccharomyces cerevisiae/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
Four cycloartane triterpene oligoglycosides were isolated from the n-butanol extract of the aerial parts of Astragalus spinosus Vahl. (Leguminosae). They were identified as astragaloside I (1), isoastragaloside I (2), astragaloside IV (4) and cycloastragenol 6-O-glucoside (5) on the basis of comparing their m.p.'s, 1H NMR and 13C NMR spectra and chromatographic patterns with the data given in the literature. The results of AIDS antiviral and antitumor screening of the major component, astragaloside II (3), are dealt with.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Antivirais/farmacologia , Glucosídeos/farmacologia , Plantas Medicinais/química , Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD4-Positivos/microbiologia , Ensaios de Seleção de Medicamentos Antitumorais , Egito , Glucosídeos/isolamento & purificação , HIV/efeitos dos fármacos , Humanos , Técnicas In Vitro , Espectroscopia de Ressonância MagnéticaRESUMO
A spectrophotometric method is presented for assaying Senna and its preparations, for both total sennosides and total rhein glycosides content. The method ensures complete elimination of other minor non-carboxylic anthracene derivatives, as well as flavonoidal contaminants. The proposed method quantitates the actual total sennosides content, through the elimination of these contaminants, and through correction for the interference due to the coexistence of rhein with sennidins, in the final determinative step. This would eliminate false high figures for total sennosides by earlier procedures and reflects, perspectively, the actual potency of the assayed samples.
Assuntos
Antraquinonas/análise , Glicosídeos/análise , Extrato de Senna/análise , Glucosídeos/análise , Senosídeos , Espectrofotometria/métodos , Comprimidos/análiseRESUMO
A new colorimetric method for glycyrrhizin in licorice and drug preparations is given. The method is based on coupling the acidic genin, glycyrrhetinic acid, with methylene blue and measuring the extinction of the coupled compound solution in chloroform-alcohol.
